Spermidine Binding to the Acetinobacter baumannii Efﬂux Protein AceI Observed by Near-UV Synchrotron Radiation Circular Dichroism Spectroscopy

Simple Summary: The aim of this work was to use highly intense light produced by a synchrotron facility to detect binding of the polyamine compound spermidine to a protein called AceI from the pathogenic bacterium Acinetobacter baumannii , which is a leading cause of hospital-acquired infections. AceI is an efﬂux pump in the cell membrane of A. baumannii that extrudes the antiseptic compound chlorhexidine from the cell. Efﬂux pumps are one of the major mechanisms that bacteria use for resistance against antimicrobial agents such as chlorhexidine. Because chlorhexidine is an industrially produced compound, it is not a natural substrate of AceI. This work was the start of our investigation into whether polyamine compounds such as spermidine are naturally occurring substrates of AceI. Abstract: The aim of this work was to test polyamines as potential natural substrates of the Acinetobacter baumannii chlorhexidine efﬂux protein AceI using near-UV synchrotron radiation circular dichroism (SRCD) spectroscopy. The Gram-negative bacterium A. Baumannii is a leading cause of hospital-acquired infections and an important foodborne pathogen. A. Baumannii strains are becoming increasingly resistant to antimicrobial agents, including the synthetic antiseptic chlorhexidine. AceI (144-residues) was the founding member of the recently recognised PACE family of bacterial multidrug efﬂux proteins. Using the plasmid construct pTTQ18-aceI(His 6 ) containing the A. baumannii aceI gene directly upstream from a His 6 -tag coding sequence, expression of AceI(His 6 ) was ampliﬁed in E. coli BL21(DE3) cells. Near-UV (250–340 nm) SRCD measurements were performed on detergent-solubilised and puriﬁed AceI(His 6 ) at 20 ◦ C. Sample and SRCD experimental conditions were identiﬁed that detected binding of the triamine spermidine to AceI(His 6 ). In a titration with spermidine (0–10 mM), this binding was saturable and ﬁtting of the curve for the change in signal intensity produced an apparent binding afﬁnity ( K D ) of 3.97 ± 0.45 mM. These SRCD results were the ﬁrst experimental evidence obtained for polyamines as natural substrates of PACE proteins.

The 144-residue AceI protein (Acinetobacter chlorhexidine efflux protein I) (https: //www.uniprot.org/uniprot/P0DUT9, accessed on 20 March 2022) was the founding member of the PACE (proteobacterial antimicrobial compound efflux) family of bacterial multidrug efflux proteins [5,6]. PACE proteins contain~150 amino acids and sequence analysis suggests that they form four transmembrane-spanning α-helices arranged as two tandem bacterial transmembrane pair (BTP) domains [7]. The relatively small size of PACE proteins means that they are likely to function as an oligomer. A mass spectrometry study demonstrated that AceI exists in a monomer-dimer equilibrium in solution, where AceI forms dimers at high pH and binding to chlorhexidine facilitates the functional form of the protein [8]. A high-resolution three-dimensional structure of a PACE protein is yet to be elucidated.
Because chlorhexidine is a synthetic biocide used only since the 1950s, chlorhexidine efflux will not be the physiological function of AceI, so the resistance to chlorhexidine that PACE proteins provide is fortuitous. Possible natural substrates of AceI that we investigated included polyamines such as spermidine, spermine, putrescine and cadaverine that have some chemical and structural similarity to chlorhexidine. Polyamines are primordial compounds found abundantly in eukaryotes, bacteria and archaea [9], and they have multiple roles in bacterial pathogens [10,11]. Polyamines may exist at high (millimolar) concentrations in cells, and they can inhibit cell growth when they are in excess, so it makes sense for cells to have detoxification mechanisms for polyamines such as active efflux.
One of the first types of biophysical experimental evidence that we obtained for chlorhexidine binding to AceI was performed through near-UV synchrotron radiation circular dichroism (SRCD) spectroscopy using methods developed with and applied to other membrane proteins [12][13][14][15][16]. By this method, chlorhexidine binding to detergentsolubilised and purified AceI had an apparent binding affinity (K D ) of 5.8 µM [4]. We therefore used near-UV SRCD spectroscopy to test for polyamines binding to AceI, and here we report the first experimental evidence obtained for a polyamine binding to a PACE protein.

Near-UV SRCD Spectroscopy
Measurements were performed using a nitrogen-flushed CD instrument on Beamline B23 at the Diamond Light Source Ltd. (Oxfordshire, UK) [18,19]. Samples contained purified AceI(His 6 ) (20 µM or 40 µM) in potassium phosphate buffer (10 mM, pH 7.6) plus 5% glycerol and 0.05% DDM. The sample cell had a path length of 1 cm and aperture 2 mm (minimum volume 60 µL) and used a 1 mm collimated beam. Spectra in the wavelength range 250-340 nm were obtained at 20 • C using slit widths of 1 mm, increment 0.5 nm and integration time of 1 s. All spectra were an average of 10 scans acquired over approximately 1 h. Spectra were zeroed at a wavelength of 335 nm and corrected for any signals coming from buffer or from the added polyamines themselves by subtraction of the relevant spectra acquired on these sample components alone, as appropriate. Spectra are presented in units of mean residue ellipticity ([θ]MRE, deg.cm 2 .dmol −1 ) and all measurements had PMT values below 600 V. For quantifying spermidine binding, the polyamine was titrated with AceI up to a concentration of 10 mM. At each titration point, the sample cuvette was removed from the instrument, an appropriate volume of a higher concentration spermidine solution was added, followed by gentle mixing with a pipette before returning to the instrument and recording the next spectrum. The change in [θ]MRE at each titration point was measured at six different wavelengths in the region 255-260 nm. Average values for the change in [θ]MRE were fitted to the Michaelis-Menten equation using GraphPad Prism 6 software to produce the apparent dissociation constant. Stability measurements confirmed that there was no change in spectra of the protein alone when continuously measured over a period of 12 h, which is longer than that of an entire titration with ligand.

Results and Discussion
Using a trial and error approach, sample and near-UV SRCD experimental conditions were identified and optimised for observing the triamine spermidine binding to DDMsolubilised and purified AceI(His 6 ). At concentrations of 20 µM and 40 µM, AceI(His 6 ) the binding of 5 mM spermidine was detected in spectra recorded in the near-UV region (250-340 nm), with the most prominent effects on the spectra at 255-295 nm. Under the same conditions, binding of the longer-chain tetraamine spermine was not detected (Figure 1). SRCD spectra of proteins in the near-UV region represent and are sensitive to changes in their tertiary structure, which can be influenced by their conformational state, mobility and environment. During our extensive experience in developing and performing SRCD experiments on various membrane proteins, we have noticed that separate preparations of the same protein under the same sample conditions can produce starting near-UV spectra with different shapes. In these types of experiments, it is therefore important to monitor any changes in the shape and magnitude of spectra in the same sample over time or on addition of ligands, rather than comparing spectra coming from different preparations.
For protein samples, SRCD spectra in the near-UV region especially report information from the aromatic groups of phenylalanine (255-270 nm), tyrosine (275-282 nm) and tryptophan (290-305 nm) residues, with fine structure originating from vibronic transitions [20]. The 144 residues of AceI contain 12 (8.3%) phenylalanine, 6 (4.2%) tyrosine and 3 (2.1%) tryptophan residues. The contents of phenylalanine and tyrosine residues are notably high when compared with their average contents in secondary transport proteins from Escherichia coli (6.1% and 2.7%, respectively) [21]. The high content of phenylalanines in AceI gives them a greater chance of undergoing conformational changes on the binding of a ligand that can be detected in the spectra. In order to quantify the binding of spermidine, a titration of 40 µM AceI(His 6 ) with spermidine up to 10 mM was performed (Figure 1). Stability measurements confirmed that there was no change in spectra of the protein alone when continuously measured over a period of 12 h, which is longer than the entire titration with ligand. was performed (Figure 1). Stability measurements confirmed that there was no change in spectra of the protein alone when continuously measured over a period of 12 h, which is longer than the entire titration with ligand. All spectra are an average of 10 scans acquired over approximately 1 h. Spectra were corrected for any signals coming from buffer or from the polyamines themselves by subtraction of the relevant spectra acquired on these sample components alone, as appropriate.
A saturable change in signal intensity on titration with spermidine was measured in the region 255-260 nm, which corresponds to the wavelength range for phenylalanine residues. Fitting a mean of the change in signal intensity in this region gave an apparent binding affinity (KD) of 3.97 ± 0.45 mM (Figure 2). Whilst the binding of spermidine to AceI has a three-fold lower affinity than the binding of chlorhexidine, a lower binding affinity for a natural substrate of a transporter is expected. The SRCD results presented here were the first experimental evidence obtained for a polyamine binding to a PACE protein. All spectra are an average of 10 scans acquired over approximately 1 h. Spectra were corrected for any signals coming from buffer or from the polyamines themselves by subtraction of the relevant spectra acquired on these sample components alone, as appropriate.
A saturable change in signal intensity on titration with spermidine was measured in the region 255-260 nm, which corresponds to the wavelength range for phenylalanine residues. Fitting a mean of the change in signal intensity in this region gave an apparent binding affinity (K D ) of 3.97 ± 0.45 mM (Figure 2). Whilst the binding of spermidine to AceI has a three-fold lower affinity than the binding of chlorhexidine, a lower binding affinity for a natural substrate of a transporter is expected. The SRCD results presented here were the first experimental evidence obtained for a polyamine binding to a PACE protein. . Stability measurements confirmed that there was no significant change in spectra of the protein alone when continuously measured over a period of 12 h, which was longer than that of an entire titration with spermidine.

Conclusions
CD measurements on proteins in the near-UV region that report on tertiary structure can be used to detect ligand binding, which may not be possible by using CD in the far-UV region or by other biophysical techniques (e.g., fluorimetry). Due to insufficient sensitivity, such CD measurements in the near-UV region are not possible using a benchtop instrument, so a longer pathlength of 1 cm and the intense light produced by synchrotron radiation are required to detect the near-UV spectra of membrane proteins. The results presented in this paper initiated further study on polyamines as potential natural substrates of AceI and other PACE proteins. Future work on the relatively uncharacterised PACE family should include gene cloning, recombinant expression, purification, reconstitution and stability screening of further PACE proteins for application of a multitude of chemical, biochemical, biophysical and computational techniques to elucidate high-resolution structures and to investigate their oligomeric state, physiological function, ligand interactions and substrate specificity, molecular mechanism and dynamics. This may provide information to assist the development of agents or strategies to block the biocide efflux function of PACE proteins.

Data Availability Statement:
The data that support this study are presented in the manuscript or available from the corresponding author upon reasonable request. . Stability measurements confirmed that there was no significant change in spectra of the protein alone when continuously measured over a period of 12 h, which was longer than that of an entire titration with spermidine.

Conclusions
CD measurements on proteins in the near-UV region that report on tertiary structure can be used to detect ligand binding, which may not be possible by using CD in the far-UV region or by other biophysical techniques (e.g., fluorimetry). Due to insufficient sensitivity, such CD measurements in the near-UV region are not possible using a benchtop instrument, so a longer pathlength of 1 cm and the intense light produced by synchrotron radiation are required to detect the near-UV spectra of membrane proteins. The results presented in this paper initiated further study on polyamines as potential natural substrates of AceI and other PACE proteins. Future work on the relatively uncharacterised PACE family should include gene cloning, recombinant expression, purification, reconstitution and stability screening of further PACE proteins for application of a multitude of chemical, biochemical, biophysical and computational techniques to elucidate high-resolution structures and to investigate their oligomeric state, physiological function, ligand interactions and substrate specificity, molecular mechanism and dynamics. This may provide information to assist the development of agents or strategies to block the biocide efflux function of PACE proteins.