The Same Magnocellular Neurons Send Axon Collaterals to the Posterior Pituitary and Retina or to the Posterior Pituitary and Autonomic Preganglionic Centers of the Eye in Rats

: In rats, some parvocellular paraventricular neurons project to spinal autonomic centers. Using the virus tracing technique, we have demonstrated that some magnocellular paraventricular neurons, but not supraoptic neurons, also project to autonomic preganglionic centers of the mammary gland, gingiva, or lip. A part of these neurons has shown oxytocin immunoreactivity. In the present experiment, we have examined whether the same magnocellular neuron that sends ﬁbers to the retina or autonomic preganglionic centers of the eye also projects to the posterior pituitary. Double neurotropic viral labeling and oxytocin immunohistochemistry were used. After inoculation of the posterior pituitary and the eye with viruses, spreading in a retrograde direction and expressing different ﬂuorescence proteins, we looked for double-labeled neurons in paraventricular and supraoptic nuclei. Double-labeled neurons were observed in non-sympathectomized and cervical-sympathectomized animals. Some double-labeled neurons contained oxytocin. After the optic nerve was cut, the labeling did not appear in the supraoptic nucleus; however, it could still be observed in the paraventricular nucleus. In the paraventricular nucleus, the double-labeled cells may be the origin of centrifugal visual ﬁbers or autonomic premotor neurons. In the supraoptic nucleus, all double-labeled neurons are cells of origin of centrifugal visual ﬁbers.


Introduction
Two retinofugal systems have long been known by researchers. One of them is the classical visual system [1], and the other one is the retinohypothalamic tract [2,3]. In 1893, Ramon Y Cajal [4] discovered fibers in the retina, which, according to his opinion, might originate from the central nervous system. These fibers were named centrifugal visual fibers. Repérant and coworkers [5] overviewed the data concerning the centrifugal visual system. It was found that "the centrifugal visual fibers do not form a homogenous system, rather they form at least eight subsystems". Vereczki and her coworkers [6] injected biotinylated dextran amine (BDA) into the vitreous body of the eye of rats. They found BDA-containing neurons in supraoptic (SON) and paraventricular (PVN) nuclei, besides several forebrain structures. It was concluded that these labeled neurons might be the cells of origin of centrifugal visual fibers.
Ample evidence indicates that hypothalamic nuclei are involved in the control of autonomic innervation of various organs. First, nontranssynaptic tract-tracing techniques were used; it was found that some parvocellular PVN (pPVN) neurons project to the spinal cord. Secretory granules were not found in these neurons [7]. Swanson and Kuypers [8] different chemical natures, and they can exert the fine-tuning of neuronal networks that are involved in social recognition, pair-bonding, stress, food intake, fear, trust, empathy, and xonophobia.
No convincing data are available in the literature about whether the same posterior pituitary projecting neurons (PPPNs) send axon collaterals to the preganglionic autonomic neurons of the eye, located in the Edinger-Westfal (E-W) nucleus or the IML of the spinal cord. Nothing is known about whether the PPPNs also give rise to centrifugal visual fibers.
In our present experiment, the above-mentioned possibilities were investigated using the dual virus track tracing method. After inoculation of the PP with a virus and the inoculation of the eye with viruses expressing different fluorescence proteins (green: Ka-gEI-memGFP-RV; red: Ka-Vhs-memCherry-A-RV or Ka-Vhs-CHR-memTomato-A-RV), we looked for double-labeled neurons in the PVN and SON. With the use of immunohistochemistry, we also examined whether the double-labeled neurons contained OT. The reason for this experiment is that OT has been described in the extracellular matrix between the retinal cones and OT receptors have been demonstrated on the retinal pigment epithelium [21,22], but the origin of OT has not been identified. We wanted to find out whether there are enough OT neurons, which project to the retina, and whether they could explain the source of this peptide. As it has clearly been shown that AVP has an intrinsic system in the retina [23], we did not examine AVP immunoreactivity in the double-labeled neurons.

Viruses Used for Inoculation
Three pseudorabies virus strains were used (constructed by the coauthors Zsolt Boldogkői and Dóra Tombácz). Ka-gEI-memGFP-RV could spread only in a retrograde direction and expressed green fluorescence protein (GFP). Ka-Vhs-memCherry-A-RV or Ka-Vhs-CHR-memTomato-A-RV could be transported in both ante-and retrograde directions and expressed red fluorescence protein (Cherry or Tomato). The viruses were kept on Medusa cells in a DMEM solution (Gibco/Thermo Fisher Scientific, Waltham, MA USA), supplemented with 5% fetal bovine serum (Gibco/Thermo Fisher Scientific, Waltham, MA, USA) and 80 µg/mL gentamycin (8 × 10 8 PFU/mL). Ka-gEI-memGFP-RV was injected into the vitreous body of the eye at the corneo-scleral junction or into the pituitary. Ka-Vhs-memCherry-A-RV or Ka-Vhs-CHR-memTomato-A-RV was injected into the pituitary. Csáki and coworkers [24] confirmed the only retrograde or ante-retrograde transportation of our virus strains.

Inoculation of the Pituitary Gland
Under general anesthesia (chloral hydrate 35mg/100g bw), the animals were placed in a special stereotaxic instrument (commonly used for transauricular hypophysectomy). A hollow ear bar with a trocar inside was inserted into the right external auditory canal and pushed through the middle and inner ear toward the sella turcica. Through a glass tube that was inserted into the ear bar instead of the trocar, the animals received 2 µL of one of the viruses into the pituitary gland. From the PP, the virus could have been transported only in a retrograde manner because, in this organ, neuronal cell bodies are not present. Nerve terminals picked up the virus and carried it toward the hypothalamus and along the sympathetic nerve fibers, which were also observed in the PP [25,26]. The anterior pituitary itself does not contain nerve endings coming from the hypothalamus. Only the brains of those animals were further processed, where the sellar dura and the median eminence (ME) were not injured (this fact was confirmed at autopsy).

Sympathectomy
To prevent labeling through sympathetic fibers of the PP or sellar dura, we have also used cervical sympathectomized rats. Under general anesthesia, both superior cervical ganglion (SCG) were excised. The success of the surgery was proven by the appearance of ptosis, myosis, enophthalmos, and the lack of labeling in the IML of the corresponding spinal thoracic segment.

Optic Nerve Cut
Under general anesthesia, the animals were placed in a stereotaxic instrument in a nose-down position (3.5 mm). A piece of razor blade was fixed onto the holder. Its width was 2 mm. The blade was lowered into the brain through a horizontal fissure drilled in the skull. When the blade reached the inner surface of the base of the skull, the right optic nerve was interrupted; then, the blade was removed. The coordinate was A10.4mm in front of the interaural line (IA), according to "The Rat Brain in Stereotaxic Coordinates" by Paxinos and Watson [27]. The rostral part of the hypothalamus, between A7mm and A11mm, was sectioned on the horizontal plain to check the location of the nerve injury.

Experimental Groups
Intact (non-sympathectomized) and sympathectomized animals were used. Labeling was sought in the hypothalamus and autonomic centers. The following groups were included in the experiments:  (No = 7; sympathetic, parasympathetic, and centrifugal visual fibers remain intact). • Group 6: Ka-gEI-memGFP-RV injection into the vitreous body of the eye of sympathectomized rats and Ka-Vhs-CHR-memTomato-A-RV injection into the pituitary (No = 7; parasympathetic and centrifugal visual fibers remain intact). • Group 7: Ka-gEI-memGFP-RV injection into the vitreous body of eye of rats bearing the right optic nerve cut (No = 3; all autonomic fibers remain intact).

Preparing Tissues for Investigations
Three days after inoculation, the animals were anesthetized and perfused with 4% paraformaldehyde (PFA) in potassium phosphate buffer (KPB; 0.05Mol, pH7.2; Sigma, St. Louis, MO, USA). The brain, spinal cord, SCG, and ciliary ganglion (CilG) were removed, postfixed in PFA, then washed in KPB. The tissue blocks were treated with sucrose solution (Merck, Darmstadt, Germany) for cryoprotection and embedded in Tissue-Tek (Sakura Finetek Europe B. V., Rijn, the Netherlands). Frozen sections were made with a cryotome (Thermo Shandon, Pittsburg, PA, USA). The slides were examined under a confocal fluorescence microscope (Radiance 2100 Rainbow Multiphoton Imaging System, Bio-Rad Laboratories, Hercules, CA, USA) using Zen 2012 software.

Immunohistochemistry
Sections of the hypothalamus, where double virus labeling was observed, were stained for OT immunoreactivity. First, the sections were washed in KPB and treated with 1% Triton X-100 (Reanal, Budapest, Hungary) for better penetration of the antibody. Then, the sections were treated with 1% serum bovine albumin (Merck, Darmstadt, Germany) to diminish the background staining. After washing the slides three times in KPB OT-N, the monoclonal antibody, raised in mice and characterized by Ben-Barak and coworkers [28], was used in a dilution of 1:100. The sections were incubated in the antibody solution for 48 h at room temperature, and, after washing the slides three times in KPB, they were further incubated in goat anti-mouse biotinylated antibody for an hour (Vector, Burlingame, CA, USA). The final reaction product was visualized with aminomethylcoumarin acetate (AMCA; Jackson ImmunoResearch, Baltimore, MD, USA; 2-h incubation) to test the specificity of the OT-N antibody, the primary, and, in the other case, the biotinylated second antibody; alternatively, both were left out.

Specificity of Labeling
The specificity of the only retrograde or ante-retrograde spreading of our virus strains was tested in a previous paper [24]. Injection of the virus into blood resulted in no labeling anywhere in the central nervous system; this was also demonstrated in the same previous paper. By leaving the primary antibody out in the OT-N immunostaining, labeling was not observed. As a positive control, OT-N antibody characteristically stained neurons in the magnocellular PVN and SON.

Evaluation of the Number of PVN and SON Neurons Labeled by Virus from the Eye
From the hypothalamus of all experimental groups, starting at 7 mm in front of the IA, two parallel series of 20 µm thick frontal sections were prepared. In every series, we had 15 sections (one from each 40 µm thick tissue). The sections were mounted on gelatinized slidesin four to eight sections of the first series Ka-gEI-memGFP-RV labeled cells were counted, and then the double-virus-labeled cells were counted. As there was no difference in the data between male and female rats their data were analysed together. Data of intact and sympathectomized rats were compared. The data were subjected to one-way analysis of variance (ANOVA). Tukey's test was used as a post-test. Statistical significance was defined as p < 0.05. The graphs were created by the GraphPad Prism 7 program. All values in the figures are expressed as mean ± SEM.

Labeling Induced by Pituitary Inoculation
Pituitary inoculation of intact and sympathectomized rats with Ka-gEI-memGFP-RV (Group 1 and 2) resulted in the labeling of the SON ( Figure 1A) and PVN ( Figure 1B) on both sides. In intact rats, labeling was observed mainly in the lateral part of the PVN (mPVN). As the pituitary gland and the sellar dura are innervated by sympathetic fibers; via these fibers, the virus is transported to the spinal autonomic center, the IML (T1-3 segments of the spinal cord; Figure 1C); then, from there, the virus might also reach some neurons in the PVN in a retrograde manner ( Figure 1B). Sympathectomy did not considerably influence the labeling in the SON ( Figure 1D) and PVN ( Figure 1E); however, it did prevent labeling in the IML ( Figure 1F).
Pituitary inoculation of intact and sympathectomized rats with Ka-Vhs-memCherry-A-RV or Ka-Vhs-CHR-memTomato-A-RV (Groups 3 and 4) also induced labeling in the magnocellular system on both sides ( Figure 1G,H). Labeling was not observed in the pPVN or any other parvocellular region of the hypothalamus ( Figure 1I). The virus reached the second-order autonomic cell groups: the E-W nucleus (E-W; Figure 1J) and IML ( Figure 1K). Pituitary inoculation of sympathectomized rats also resulted in the labeling of the SON ( Figure 1L), mPVN ( Figure 1M), and E-W nucleus ( Figure 1N) but did not appear in the IML ( Figure 1O).
(mPVN). As the pituitary gland and the sellar dura are innervated by sympatheti via these fibers, the virus is transported to the spinal autonomic center, the IM segments of the spinal cord; Figure 1C); then, from there, the virus might als some neurons in the PVN in a retrograde manner ( Figure 1B). Sympathectomy considerably influence the labeling in the SON ( Figure 1D) and PVN ( Figure 1E ever, it did prevent labeling in the IML ( Figure 1F).

Labeling, Focused on PVN, after Pituitary and Eye Inoculations (Groups 5 and 6)
In intact rats (Group 5), Ka-gEI-memGFP-RV from the eye was transported in a retrograde manner to the postganglionic SCG ( Figure 2A) and CilG ( Figure 2B), then to the preganglionic neurons of IML (T2; Figure 2C) and E-W nucleus ( Figure 2D). Finally, the virus reached the premotor neurons located in the hypothalamic PVN on both sides ( Figure 2E). In sympathectomized rats (Group 6) after eye inoculation with Ka-gEI-memGFP-RV, labeling was observed in the optic nerve ( Figure 3A) and the parasympathetic CilG (Figure 3B). The virus was further transported via the oculomotor nerve ( Figure 3C) to the E-W nucleus ( Figure 3D); however, sympathectomy prevented the labeling in the sympathetic preganglionic neurons of the IML ( Figure 3E). In the PVN ( Figure 3F) and SON ( Figure 3G), labeling was always found. The number of Ka-gEI-memGFP-RV-labeled cells   Figure 2Fb demonstrates the histological appearance of the injection site. Through the anterior lobe, the glass capillary reached the PP, where the virus was taken up by the fibers and was transported through the pituitary stalk to the hypothalamus. In the mPVN, double-labeled neurons were observed ( Figure 2G-I). Figure 2J shows a highly magnified detail of Figure 2I. Figure 2K shows the hypothalamic level according to Paxinos and Watson's brain stereotaxic atlas, where double labeling is demonstrated in the mPVN. Figure 2L-N shows other double-labeled cells in the mPVN; Figure 2O shows a highly magnified detail of Figure 2N.
In sympathectomized rats (Group 6) after eye inoculation with Ka-gEI-memGFP-RV, labeling was observed in the optic nerve ( Figure 3A) and the parasympathetic CilG ( Figure 3B). The virus was further transported via the oculomotor nerve ( Figure 3C) to the E-W nucleus ( Figure 3D); however, sympathectomy prevented the labeling in the sympathetic preganglionic neurons of the IML ( Figure 3E). In the PVN ( Figure 3F) and SON ( Figure 3G), labeling was always found. The number of Ka-gEI-memGFP-RV-labeled cells in the PVN was lower in sympathectomized rats then in intact rats. The difference was statistically significant. However, in the SON, there was no difference in the number of GFP-labeled cells between the two groups ( Figure 3H). In the SON, the number of GFP-labeled neurons was significantly lower than that in the PVN of either the intact or sympathectomized rats.
As was expected, the Ka-Vhs-CHR-memTomato-A-RV virus signal was also observed in the SON ( Figure 4A) and mPVN ( Figure 4B). Labeling descended from the mPVN to the E-W nucleus in an anterograde manner ( Figure 4C). As anterograde transport is slower than the retrograde, Ka-Vhs-CHR-memTomato-A-RV did not reach CilG until three days after the inoculation. Sympathectomy prevented the labeling in the IML (not shown). In the mPVN of sympathectomized rats, similar to those in intact rats, double-labeled neurons were also found. Figure 4 shows three examples of double labeling (Figure 4D-F,G-I,J-L). The Ka-gEI-memGFP-RV injected into the eye produced labeling in the SON in both intact ( Figure 5A) and sympathectomized rats ( Figure 5B); however, interruption of the optic nerve ( Figure 5C,D) prevented SON labeling ( Figure 5E) in spite of the fact that the inoculation of the eye was successful. In these latter animals, labeling appeared in the SCG, IML ( Figure 5F), CilG, and E-W ( Figure 5G). Finally, the labeling reached the PVN as well ( Figure 5H). In the case of simultaneous inoculation with Ka-Vhs-CHR-memTomato-A-RV, double labeling was observed in the SON of intact ( Figure 5I-L,M) and sympathectomized rats ( Figure 5N-Q,R-U).
in the PVN was lower in sympathectomized rats then in intact rats. The difference was statistically significant. However, in the SON, there was no difference in the number of GFP-labeled cells between the two groups ( Figure 3H). In the SON, the number of GFPlabeled neurons was significantly lower than that in the PVN of either the intact or sympathectomized rats. As was expected, the Ka-Vhs-CHR-memTomato-A-RV virus signal was also observed in the SON ( Figure 4A) and mPVN ( Figure 4B). Labeling descended from the mPVN to the E-W nucleus in an anterograde manner ( Figure 4C). As anterograde transport is slower than the retrograde, Ka-Vhs-CHR-memTomato-A-RV did not reach CilG until three days after the inoculation. Sympathectomy prevented the labeling in the IML (not     The number of double-labeled neurons was always lower gEI-memGFP-RV-labeled neurons ( Figure 6). About one-quarter were projected to the PP as well.

Chemical Characterization of Double-Labeled Neurons in the mPV
Injection of Ka-gEI-memGFP-RV into the eye and Ka-Vhs-m PP of intact rats (Group 6) resulted in double-virus-labeled cells Immunostaining revealed that some double-virus-labeled neur immunoreactivity in both nuclei. Figure 7A-H show triple labeli of intact rats. Figure 7I-P show triple-labeled neurons in the PV thectomized rats. The number of triple-labeled neurons is much of double-labeled neurons.

Chemical Characterization of Double-Labeled Neurons in the mPVN and SON
Injection of Ka-gEI-memGFP-RV into the eye and Ka-Vhs-memCherry-A-RV into the PP of intact rats (Group 6) resulted in double-virus-labeled cells in the mPVN and SON. Immunostaining revealed that some double-virus-labeled neurons also showed OT-N immunoreactivity in both nuclei. Figure 7A-H show triple labeling in the PVN and SON of intact rats. Figure 7I-P show triple-labeled neurons in the PVN and SON of sympathectomized rats. The number of triple-labeled neurons is much lower than the number of double-labeled neurons.

Discussion
It is generally accepted that pPVN neurons project to the IML of the spinal cord. These neurons are collectively termed SPANs. SPAN cell bodies are found most densely in the mediocellular [8,9] or, according to Pyner and Coote [10], in the mid-rostral subdi-

Discussion
It is generally accepted that pPVN neurons project to the IML of the spinal cord. These neurons are collectively termed SPANs. SPAN cell bodies are found most densely in the mediocellular [8,9] or, according to Pyner and Coote [10], in the mid-rostral subdivision of the PVN. Their function is a subject of debate. Most likely, SPANs may be involved in physiological stress responses, cardiovascular control, glucose regulation, thermoregulation, and circadian rhythms [29]. The OT neurons are mainly located in the mPVN. Some pPVN neurons also show OT immunoreactivity. Althammer and Grinevich [17] have supposed that the parvocellular OT neurons do not project to the PP; however, through multiple axon collaterals, these neurons project to the hindbrain and spinal cord regions, mainly to the posterior horn, where they modulate pain perception [30]. The role of OT in social behavior, cognition, and autonomic cardiac control in psychiatric disorders of social dysfunction has been thoroughly studied, and its impact in these conditions is generally accepted [31].
In our previous experiments, we have found virus-labeled neurons not only in the pPVN but in the mPVN after injection of a retrograde traveling virus into some peripheral organs such as the mammary gland [13], lower gingiva, or lower lip of rats [14]. There is also considerable evidence that axons of the PVN neurons branch and innervate neurons that are located in at least two different places [15,16]. It was also demonstrated that oxytocinergic mPVN neurons also innervated neurons in the central amygdala, where the local release of OT mediated the unfreezing response via GABAergic interneurons [17]. In our material, only a few double-virus-labeled cells showed OT-N immunoreactivity. This fact raises the possibility that these cells operate with neuropeptides or neurotransmitters other than OT. The question arises as to what the role of the small number of oxytocinerg mPVN neurons that project to the E-W nucleus and the IML. Morphologically, a bidirectional connection exists between the PVN and the E-W nucleus. Dos Santo and coworkers [32] injected BDA into the E-W nucleus; they have found labeling in the PVN. In our experiment, Ka-Vhs-CHR-memTomato-A-RV, expressing red fluorescence protein, from the PVN descended to the E-W nucleus in an anterograde manner. As we have found double-labeled oxytocinerg neurons in sympathectomized rats, we suppose that this labeling has to belong to the centrifugal visual or premotor parasympathetic neurons, which may project to the retina or the E-W nucleus. Buijs [33] described oxytocinergic fibers in the E-W nucleus. This observation supports our hypothesis.
Our present experiment provides further insight into the anatomy of the premotor autonomic innervation of the eye. We have shown that there are neurons not only in pPVN but also the mPVN, the axons of which branch and send collaterals into the PP and simultaneously to autonomic neurons located in the E-W and IML of the spinal cord. It means that the same magnocellular neurons may function as neuroendocrine cells and sympathetic and parasympathetic premotor neurons. We named these neurons autonomic premotor PPPNs.
In the PVN and SON, we would have liked to know how many percent of virus-labeled neurons received a virus from the sympathetic nervous system (IML) after eye inoculation. When we injected the virus into the vitreous body of the eye, not only the retinal ganglion cells but the sympathetic and parasympathetic fibers innervating the pupillary and ciliary muscles also picked up and transported the virus. SCG and CilG were always labeled in Group 5. From here, the virus, in a retrograde manner, was transported to the IML and the E-W nucleus. It is also clear that significantly more signals hit the eye from the PVN than from the SON. The chart ( Figure 6) shows that about 45% of PVN fibers descend to parasympathetic centers or the centrifugal visual fibers, and this difference is statistically significant ( Figure 3H). In the SON, there is no difference between the sympathectomized and non-sympathectomized groups. Significantly more PVN neurons are labeled from the eye than SON neurons. This difference is due to the fact that the SON does not directly connect with the autonomic nervous system.
In another rodent (prairy vole), Eliava and coworkers [34], using tract-tracing and electrophysiological methods, identified pPVN OT neurons that project to the spinal cord and simultaneously to the PP or to other mPVN OT or SON OT neurons; in turn, these OT neurons project to the PP. They also identified pPVN OT neurons with axon collateral to the PP and another to the spinal dorsal horn. These identified connections, existing between the PVN, PP, and the dorsal horn, are involved in pain perception. It is not known whether the above observation would be true for rat ciliary autonomic centers as well as for the PVN, PP, E-W nucleus, and T2 IML. Not oxytocin fibers but oxytocin receptors were also demonstrated in another rodent. Wrobel and coworkers [35], using OT-receptor-reporter mice, in which a fluorescent protein (Venus) was expressed, found OT receptors in about 5% of preganglionic IML neurons.
After eye inoculation, we have found viral-labeled neurons in the SON. This observation correlates well with our previous data. After injection of the vitreous body with BDA, retrograde-labeled neuronal cell bodies appeared in the SON and PVN. It was concluded that the labeled neurons sent centrifugal visual fibers to the retina [6]. There are no data in the literature that indicate that SON does directly send premotor fibers to the autonomic centers. We have never observed labeling in this nucleus after the gingiva, lip [14], and adrenal inoculations (unpublished data). The finding that an interruption of the right optic nerve (the retrograde virus was injected into the right eye) prevented the labeling in the SON, but not in the PVN, strongly supports the above-mentioned observation. In these animals, the tracer, injected into the eye, could reach the PVN through the CilG and E-W or through SCG and IML, but not through the optic nerve. In our sympathectomized rats, the persisting retrograde fiber labeling in the optic nerve also suggests that these fibers belong to the centrifugal visual system. These phenomena also support our hypothesis that the virus-labeled neurons in the SON are the cells of origin of the centrifugal visual fibers. The labeled neurons in the PVN of intact animals may belong to sympathetic and parasympathetic premotor systems, and these cells may be the origin of the centrifugal visual system.
There is information on the roles of OT and AVP in the control of the eye autonomic nervous system. It is well established the PVN sends information to the E-W nucleus and the corresponding IML; the E-W nucleus innervates the CilG; the IML innervates the SCG. In turn, the CilG and SCG are responsible for pupil mimicry and lens accommodation and influence choroidal blood flow. A research group [36] observed that when two people talk to one another at close range, they tend to trust one another more if their pupils are dilated or less if there is constriction. In males, the application of intranasal OT enhanced trust; in females, OT depressed trust. In rabbits, intravitreal administration of a very low dose (10 −10 M) of AVP induced pupillary constriction [37].
The role of OT in the retina was investigated by Halbach and coworkers [21]. They have described that OT is present in the cone photoreceptor extracellular matrix and the OT receptor is present in the adjacent retinal pigment epithelium. OT is a paracrine signaling molecule and induces an increase in the retinal pigment epithelium [Ca2+]i level via the activation of the OT receptor. These results do not explain without doubt the origin of OT in the retina. We have found several OT immunoreactive double-labeled cells in the SON. These cells may be the source of OT in the retina. Althammer and coworkers [19] claimed that only a few molecules might have functional consequences because the OT receptors exhibit a high affinity for their ligand. Another nonapeptide, AVP, was also found in the retina. Its level is very low, but a special technique, using AVP-eGFP transgenic rats, was able to demonstrate AVP immunoreactive cells in the ganglion cell layer and the inner nuclear layer. AVP mRNA in these cells was also shown. V1a and V1b receptors were also found in the retina. The authors concluded that AVP-synthesizing and AVP-receiving mechanisms can operate in a paracrine manner [23]. Figure 8 illustrates our hypothesis.

Conclusion
(1) The same mPVN neurons that project to the PP may be the cell of trifugal visual fibers or autonomic premotor neurons. (2) The double-la neurons are not only sympathetic but parasympathetic as well because sim tions were also made in intact and sympathectomized rats.