Catalase Like-Activity of Metal NPs–Enzyme Biohybrids

: In this work, an efﬁcient synthesis of bionanohybrids as artiﬁcial metalloenzymes (Cu, Pd, Ag, Mn) based on the application of an enzyme as a scaffold was described. Here we evaluated the effect of changing the metal, pH of the medium, and the amount of enzyme in the synthesis of these artiﬁcial metalloenzymes, where changes in the metal species and the size of the nanoparticles occur. These nanozymes were applied in the degradation of hydrogen peroxide for their evaluation as mimetics of catalase activity, the best being the Mn@CALB-H 2 O , which presented MnO 2 nanostructures, with three-fold improved activity compared to Cu 2 O CuNPs@CALB-P free


Introduction
Hydrogen peroxide (H 2 O 2 ) is a by-product produced within the cells of living organisms and has detrimental effects on them [1][2][3][4]. Catalases are enzymes used by cells to remove excess cytoplasmic hydrogen peroxide by converting them to water and molecular oxygen. However, if the concentration of H 2 O 2 increases due to disease, radiation exposure, and certain chemicals and medications that exceed the capacity of catalase, H 2 O 2 begins to accumulate and cause cell damage [5][6][7][8]. On the other hand, the activity of the catalase enzyme can be affected by different environmental conditions, such as ionic strength, pH, and temperature (T), or due to the presence of compounds that inhibit its activity [9,10].
The rapid development of nanotechnology in recent decades has led to the creation of numerous catalytically active nanomaterials [11]. Currently, nanomaterials of different natures are widely used in biology, medicine, and biotechnology. This category includes nanomaterials with enzyme-mimetic properties, such as metallic and non-metallic nanoparticles, their oxides, magnetic nanoparticles, liposomes, and polymeric and carbon-based materials [12][13][14]. The ability of these nanomaterials to replace specific enzymes may offer new opportunities for enzyme-based applications such as immunoassays, biosensors, pharmaceutical processes, oncotherapy, the food industry, ecology, etc. [15][16][17][18]. This demonstrates the great importance of and commercial interest in the use of nanomaterials as enzyme mimetics. Compared with natural enzymes, nanozymes with inherent enzyme activities have recently attracted considerable attention due to their easy preparation, storage, and separation, as well as low cost [18,19].
One of the most used metals in the formation of artificial metalloenzymes is copper, due to its abundance, low cost, and low toxicity. Although most of the activities described in the literature are for Cu(II) materials, in fact, recently, in our group, Cu(II)-nanozymes with catalase activity were developed [20].
In this work, we describe the synthesis of highly stable heterogeneous metal (Cu, Mn, Pd, and Ag) nanoparticle catalysts (metal NPs-enzyme biohybrids), where the NPs were created in situ from an aqueous solution of the metal salt ( Figure 1A) [21,22]. In addition, we have shown that these bionanohybrids of different natures, for example, other copper species and different metals, behaviors, and sizes, have the ability to completely mimic the particular enzymatic activity of catalase, even improving its activity ( Figure 1B).

Synthesis of CuNPs@CALB Bionanohybrids
A total of 3.6 mL of commercial lipase B from Candida antarctica solution (CALB; 10 mg/mL) was added to 60 mL buffer 0.1 M (sodium phosphate pH 7.0) in a 250 mL glass bottle containing a small magnetic bar stirrer. Then, 600 mg of Cu2SO4 × 5H2O (10 mg/mL) was added to the protein solution, and it was maintained for 16 h. After 16 h, 6 mL of NaBH4 (300 mg) aqueous solution (1.2 M) was added to the cloudy solution (in two amounts of 3 mL). The solution turned rapidly black, and the mixture was reduced for 30 min. After the incubation, in all cases, the mixture was centrifuged at 8000 rpm for 5 min

Synthesis of CuNPs@CALB Bionanohybrids
A total of 3.6 mL of commercial lipase B from Candida antarctica solution (CALB; 10 mg/mL) was added to 60 mL buffer 0.1 M (sodium phosphate pH 7.0) in a 250 mL glass bottle containing a small magnetic bar stirrer. Then, 600 mg of Cu 2 SO 4 × 5H 2 O (10 mg/mL) was added to the protein solution, and it was maintained for 16 h. After 16 h, 6 mL of NaBH 4 (300 mg) aqueous solution (1.2 M) was added to the cloudy solution (in two amounts of 3 mL). The solution turned rapidly black, and the mixture was reduced for 30 min. After the incubation, in all cases, the mixture was centrifuged at 8000 rpm for 5 min (10 mL per falcon-type tube of 15 mL). The generated pellet was re-suspended in 15 mL of distilled water. It was centrifuged again at 8000 rpm for 5 min at RT, and the supernatant removed. The process was repeated twice more. Finally, the supernatant was removed, and the pellet was re-suspended in 2 mL of distilled water, transferred to a cryotube, frozen with liquid nitrogen, and lyophilized overnight. After that, approximately 150 mg of black solid was obtained. The hybrid was called CuNPs@CALB-P. Another variation of the protocol was avoiding the lyophilization step, conserving the catalyst as liquid suspension. This was called CuNPs@CALB-P-NL.
Other hybrids were synthesized following the previously established protocols under different conditions of pH, drying, and reduction (Table S1) which were tested in our work [23]. These bionanohybrids were called CuNPs@CALB-P*, CuNPs@CALB-B, and CuNPs@CALB-B*.

Synthesis of MnNPs@CALB Bionanohybrids
A total of 3 mL of commercial CALB solution (10 mg/mL) was added to 100 mL sodium phosphate buffer 0.1 M pH 7.0 in a 250 mL glass bottle containing a small magnetic bar stirrer. Then, 70 mg of MnSO 4 × 5H 2 O (0.7 mg/mL) was added to the protein solution and maintained for 16 h. After 16 h, 6 mL of NaBH 4 (300 mg) aqueous solution (1.2 M) was added to the cloudy solution (in two amounts of 3 mL) as a reducing agent. The mixture was reduced for 30 min. After the incubation, the mixture was centrifuged at 8000 rpm for 5 min at RT (10 mL per falcon type tube of 15 mL). The generated pellet was re-suspended in 15 mL of distilled water. It was centrifuged again at 8000 rpm for 5 min, and the supernatant was removed. The process was repeated twice more. Finally, the supernatant was removed, and the pellet of each falcon was re-suspended in 2 mL of distilled water; all solutions were collected in a round-bottom flask, frozen with liquid nitrogen, and lyophilized for 16 h. After that, 47 mg of the MnNPs@CALB-P was obtained.
Another variation of the protocol was the omission of the reduction step using sodium phosphate buffer 25 mM at pH 8.5. After that, 58 mg of the MnNPs@CALB-P-NR was obtained.

Method 2
KMnO 4 (500 mg) was dissolved in 10 mL of DMF or distilled water. This solution was added to 40 mL of a distilled water solution containing 2 mL of CALB (10 mg/mL). The final solution was kept under gentle magnetic stirring for 24 h at room temperature (RT). After that, the resulting suspension was separated by centrifugation (10,000 rpm; 4 • C; 15 min). The recovered pellet was washed once with 10 mL of distilled water containing 20% (v/v) of the corresponding co-solvent and twice with distilled water (2 × 10 mL). After this, the suspension was directly lyophilized to obtain the catalyst as a powder for later use. After that, approx. 350 mg of each bionanohybrid was obtained, called MnNPs@CALB-H 2 O and MnNPs@CALB-DMF, respectively.

Synthesis of MeNPs@CATb Bionanohybrids
AgNO 3 (20 mg) or Na 2 PdCl 4 (10 mg) were dissolved in 5 mL of distilled water solution containing 7.5 mg of bovine catalase (CATb). The solution was kept under gentle magnetic stirring for 24 h at RT. After that, the resulting suspension was separated by centrifugation (8000 rpm; 4 • C; 15 min). The recovered pellet was washed three times with 10 mL of distilled water. Afterwards, the suspension was directly lyophilized. The bionanohybrids were called AgNPs@CATb and PdNPs@CATb.
Characterization of the different metal bionanohybrids was performed by XRD, ICP-OES, TEM, HR-TEM, and SEM analysis.

Catalase-like Activity of Metal Bionanohybrids
Hydrogen peroxide (H 2 O 2 ) (33% (w/w)) solution was prepared by adding 52 µL of hydrogen peroxide to 9.8 mL of 100 mM or 5 mM phosphate buffer (pH 6.0, pH 7.0, and pH 8.0), 5 mM tris base buffer pH 9, 5 mM MES buffer pH 5, or distilled water in order to obtaining a final concentration of 50 mM. The solution pH was adjusted using HCl or NaOH 1 M. To start the reaction, different amounts of MeNPs hybrids (2 mg or 4.5 mg) or 50 µL of Catazyme ® 25 L (31 mg/mL) were added to 3 or 10 mL of the 50 mM solution at RT. The reaction was followed by measuring the degradation of hydrogen peroxide recording the decrease of absorbance by spectrophotometry at 240 nm in quartz cuvettes of 1 cm path length by adding 2 mL of this solution at different times. Experiments were performed in triplicate.
In order to determine the catalase activity for each catalyst, the ∆Abs/min value was calculated using the linear portion of the curve (∆Abs s ). The specific activity (U/mg) was calculated using the following equation: where the molar extinction coefficient (ε) used was 43.6 M −1 cm −1 , and mg catalyst refers to mg of enzyme or hybrid metal.

Synthesis and Characterization of MeNPs@Enzyme Biohybrids
The synthesis of MeNPs@Enzyme biohybrids was performed in an aqueous medium by using a commercial solution of lipase B from Candida antarctica (CAL-B) or bovine catalase (CATb). This enzyme was previously dissolved in distilled water, and metal salt was previously dissolved in co-solvent (20% (v/v)) or added directly to the aqueous solution at RT and under gentle stirring. After 30 min, the initial clear solution turned to a slight cloudy suspension, which was completely cloudy after 16-24 h incubation. A solid was easily obtained after centrifugation, which was washed with the same reaction solvent first and distilled water after and finally frozen in liquid nitrogen and lyophilized, yielding the MeNPs@CALB hybrid. In some cases, it was necessary to add a reduction step using NaBH 4 after incubation, since although the CALB enzyme used as scaffold induces metal reduction, in metals with low reduction potential such as transition metals such as Cu, Fe, Mn, Zn, Co, etc., an additional reduction step is needed to achieve species from lower valence states.
Firstly, CuNPs@CALB biohybrids were analyzed by XRD, demonstrating differences in species, depending on the hybrids (Figure 2A). When the bionanohybrids presented 0.6 mg/mL of enzyme, CuNPs@CALB-P and CuNPs@CALB-B were obtained. In these catalysts, almost unique copper species, Cu 2 O (matched well with JCPDS card no. 05-0667) or Cu(0) (matched well with JCPDS card no. 04-0836), respectively, could be seen. However, when the amount of enzyme was lower (0.3 mg/mL), hybrids synthesized by these protocols, CuNPs@CALB-P* and CuNPs@CALB-B*, contained both copper species, Cu 2 O and Cu(0). In the case of CuNPs@CALB-P*, it presented 40% Cu(0) (Figure 2A), and in the case of CuNPs@CALB-B*, it presented 30% Cu(I) species. These differences could be due to the effect that the enzyme performs in the reduction step, since the protein environment slows down the reduction process. The TEM analysis demonstrated the formation of nanoparticles with a different diameter size (Figures 2 and S1), in a range between 6 and 15 nm (Table S1), the smallest ones being in CuNPs@CALB-B ( Figure S1D).
Another modification of the synthesis protocol was the elimination of the lyophilization step, resulting in the production of CuNPs@CALB-P-NL. Analyzing the XRD pattern, the non-freezing of the enzyme structure could be observed; the species of this bionanohybrid presented a mixture of copper species [Cu 2 O and Cu(0) (75:25 Cu 2 O:Cu(0))]. TEM analysis demonstrated that the diameter size of the nanoparticles was the same as CuNPs@CALB-P, namely, 10 nm (Table S1).
The Cu amount on the solid was determined by ICP-OES analyses. The percentages of copper in the different CuNPs@CALB hybrids were 45% to 93% (Table S1). Appl. Nano 2022, 3, FOR PEER REVIEW 5 Another modification of the synthesis protocol was the elimination of the lyophilization step, resulting in the production of CuNPs@CALB-P-NL. Analyzing the XRD pattern, the non-freezing of the enzyme structure could be observed; the species of this bionanohybrid presented a mixture of copper species [Cu2O and Cu(0) (75:25 Cu2O:Cu(0))]. TEM analysis demonstrated that the diameter size of the nanoparticles was the same as CuNPs@CALB-P, namely, 10 nm (Table S1).
The Cu amount on the solid was determined by ICP-OES analyses. The percentages of copper in the different CuNPs@CALB hybrids were 45% to 93% (Table S1).
Subsequently, a manganese salt (MnSO4 salt) in sodium phosphate buffer was used. In this case, MnNPs@CALB-P or MnNPs@CALB-P-NR were obtained, respectively. The XRD pattern showed the same manganese species in both cases, namely, Mn3(PO4)2·3H2O ( Figure 3A) [24]. SEM analysis showed similar mesoporous structures ( Figure 3B,C). This shows that the reduction step did not have any consequence. Subsequently, a manganese salt (MnSO 4 salt) in sodium phosphate buffer was used. In this case, MnNPs@CALB-P or MnNPs@CALB-P-NR were obtained, respectively. The XRD pattern showed the same manganese species in both cases, namely, Mn 3 (PO 4 ) 2 ·3H 2 O ( Figure 3A) [24]. SEM analysis showed similar mesoporous structures ( Figure 3B,C). This shows that the reduction step did not have any consequence. In addition, bionanohybrids were also prepared using KMnO4 directly in distilled water or in the presence of DMF (20% v/v), obtaining MnNPs@CALB-H2O and MnNPs@CALB-DMF, respectively. The XRD spectrum revealed the presence of MnO2 (JCPDS card no 18-0802) in both catalysts ( Figure 4A) [25]. SEM images showed amorphous chunks, and no crystallographic plains were observed in TEM analysis ( Figure  4B,C). In addition, bionanohybrids were also prepared using KMnO 4 directly in distilled water or in the presence of DMF (20% v/v), obtaining MnNPs@CALB-H 2 O and MnNPs@CALB-DMF, respectively. The XRD spectrum revealed the presence of MnO 2 (JCPDS card no 18-0802) in both catalysts ( Figure 4A) [25]. SEM images showed amorphous chunks, and no crystallographic plains were observed in TEM analysis ( Figure 4B,C).
The amount of Mn in the solid in each case was around 25%, determined by ICP-OES analysis (Table S1).
In addition to the metal change, another variation of the system was the introduction of other enzymes as scaffold, bovine catalase (CATb). As other metals, silver (AgNO 3 ) and palladium (Na 2 PdCl 4 ) were used, to obtain AgNPs@CATb and PdNPs@CATb, respectively.
For the synthesis of the AgNPs@CATb hybrid, different Ag species were formed, namely, Ag(0), Ag 2 O, and AgO, as observed in the XRD spectrum ( Figure 5A(I)). SEM analysis demonstrated an aggregate with a mesoporous amorphous superstructure, and TEM images showed nanoparticles with a size between 10 and 20 nm due to the different species formed (Figures 5A(II,III) and S2). The amount of Ag was 34%, determined with ICP-OES analysis (Table S1). The amount of Mn in the solid in each case was around 25%, determined by ICP-OES analysis (Table S1).
In addition to the metal change, another variation of the system was the introduction of other enzymes as scaffold, bovine catalase (CATb). As other metals, silver (AgNO3) and palladium (Na2PdCl4) were used, to obtain AgNPs@CATb and PdNPs@CATb, respectively.
For the synthesis of the AgNPs@CATb hybrid, different Ag species were formed, namely, Ag(0), Ag2O, and AgO, as observed in the XRD spectrum ( Figure 5(AI)). SEM analysis demonstrated an aggregate with a mesoporous amorphous superstructure, and TEM images showed nanoparticles with a size between 10 and 20 nm due to the different species formed (Figure 5(AII,III) and S2). The amount of Ag was 34%, determined with ICP-OES analysis (Table S1). On the other hand, PdNPs@CATb was synthesized with bovine catalase as the scaffold. The XRD spectrum evidenced the existence of palladium chloride nanoparticles ( Figure 5B(I)) [26]. SEM analysis demonstrated mesoporous structures, and TEM images showed the formation of small spherical nanoparticles with an average diameter of 10 nm (Figures 5B(II,III) and S3). The amount of Pd was 27%, as determined by ICP-OES analysis (Table S1). Appl. Nano 2022, 3, FOR PEER REVIEW 8 On the other hand, PdNPs@CATb was synthesized with bovine catalase as the scaffold. The XRD spectrum evidenced the existence of palladium chloride nanoparticles (Figure 5(BI)) [26]. SEM analysis demonstrated mesoporous structures, and TEM images showed the formation of small spherical nanoparticles with an average diameter of 10 nm ( Figure 5(BII,III) and S3). The amount of Pd was 27%, as determined by ICP-OES analysis (Table S1).

Catalase-like Activity of Different MeNPs Biohybrids
Firstly, the Cu hybrids were evaluated for H2O2 degradation in distilled water ( Figure  6A). In general, all the copper catalysts demonstrated catalase activity, where CuNPs@CALB-P was the most efficient biocatalyst with specific activity of 2.5 U/mg. This was due to the presence of a unique species of copper, namely, Cu2O. Comparing this result with the rest of the copper catalysts, CuNPs@CALB-P* showed 5.5 times less activity. In the case of the catalyst synthesized at pH 10.0, it can be observed that the activity of CuNPs@CALB-B*, which had a greater presence of oxidized species, was only two times lower, while CuNPs@CALB-B, which had mostly Cu(0), presented half the activity of CuNPs@CALB-B*. The obtained results clearly demonstrated that the decomposition of hydrogen peroxide was more efficient for Cu(I) species.

Catalase-like Activity of Different MeNPs Biohybrids
Firstly, the Cu hybrids were evaluated for H 2 O 2 degradation in distilled water ( Figure 6A). In general, all the copper catalysts demonstrated catalase activity, where CuNPs@CALB-P was the most efficient biocatalyst with specific activity of 2.5 U/mg. This was due to the presence of a unique species of copper, namely, Cu 2 O. Comparing this result with the rest of the copper catalysts, CuNPs@CALB-P* showed 5.5 times less activity. In the case of the catalyst synthesized at pH 10.0, it can be observed that the activity of CuNPs@CALB-B*, which had a greater presence of oxidized species, was only two times lower, while CuNPs@CALB-B, which had mostly Cu(0), presented half the activity of CuNPs@CALB-B*. The obtained results clearly demonstrated that the decomposition of hydrogen peroxide was more efficient for Cu(I) species.
On the other hand, other tests were carried out in buffer solutions with low or high ionic strength (5 mM and 100 mM) in a pH range of 6.0 to 8.0 ( Figure 6B,C). In these cases, a general decrease in the activity of all catalysts was observed, even at very low buffer amounts. Specifically, at a low ionic strength, when the pH was higher, they presented higher catalase activity, although in the case of CuNPs@CALB-P, at any pH, it presented activity at least six times lower than in distilled water. When the medium had a high ionic strength, the results were worse for all cases.
These effect of inhibition of phosphate ions in the degradation of hydrogen peroxide by the copper nanoparticles could be explained considering some kind of binding of a phosphate group to the copper, generating intermediate species and reducing the reactivity of the catalyst. Indeed, this can be supported by the results obtained when a hybrid containing copper phosphate species [23] was used. In this case the catalase activity of this hybrid was more than 20 times lower in distilled water than the Cu hybrids present in this work (data not shown).
Therefore, for the conditions of greatest activity in CuNPs@CALB-P (distilled water), the catalase activity of the same hybrid was evaluated but without lyophilization treatment ( Figure 6D) (CuNPs@CALB-P-NL), where a 5.5-fold lower activity was observed, demonstrating the effect of the presence of Cu(0) species. Finally, the activity of CuNPs@CALB-P was compared with the activity of free catalase ( Figure 6D), where an activity of 90% could be observed.
After that, we evaluated the MnNPs@CALB hybrids under the best conditions previously studied for copper, that is, in distilled water (Figure 7). Catalysts MnNPs@CALB-P and MnNPs@CALB-P-NR, which have a manganese phosphate species, had no activity. On the other hand, other tests were carried out in buffer solutions with low or high ionic strength (5 mM and 100 mM) in a pH range of 6.0 to 8.0 ( Figure 6B,C). In these cases, a general decrease in the activity of all catalysts was observed, even at very low buffer amounts. Specifically, at a low ionic strength, when the pH was higher, they presented higher catalase activity, although in the case of CuNPs@CALB-P, at any pH, it presented activity at least six times lower than in distilled water. When the medium had a high ionic strength, the results were worse for all cases.
These effect of inhibition of phosphate ions in the degradation of hydrogen peroxide by the copper nanoparticles could be explained considering some kind of binding of a phosphate group to the copper, generating intermediate species and reducing the reactivity of the catalyst. Indeed, this can be supported by the results obtained when a hybrid containing copper phosphate species [23] was used. In this case the catalase activity of this hybrid was more than 20 times lower in distilled water than the Cu hybrids present in this work (data not shown).
Therefore, for the conditions of greatest activity in CuNPs@CALB-P (distilled water), the catalase activity of the same hybrid was evaluated but without lyophilization treatment ( Figure 6D) (CuNPs@CALB-P-NL), where a 5.5-fold lower activity was observed, demonstrating the effect of the presence of Cu(0) species. Finally, the activity of CuNPs@CALB-P was compared with the activity of free catalase ( Figure 6D), where an activity of 90% could be observed.
After that, we evaluated the MnNPs@CALB hybrids under the best conditions previously studied for copper, that is, in distilled water (Figure 7). Catalysts MnNPs@CALB-P and MnNPs@CALB-P-NR, which have a manganese phosphate species, had no activity. On the other hand, when the species were MnO2 nanostructures, MnN H2O presented the highest activity, being 2.2 times higher than MnNPs@CA Furthermore, these catalysts were evaluated in other reaction media such as base buffer pH 9 and 5 mM MES buffer pH 5 ( Figure S4). In these cases, the neg generated by the presence of ionic strength could be observed, as in the case mentioned above, where the activity in both catalysts at a pH similar to that water, such as that of the MES buffer, was 45-fold lower for MnNPs@CALB-H fold lower for MnNPs@CALB-DMF. In the case of a buffer with a higher pH MnNPs@CALB-DMF it presented similar activity, while for MnNPs@CALBhalf ( Figure S4).
Finally, the MnNPs@CALB-H2O biohybrid exhibited activity three times h that of natural catalase at RT in distilled water (Figure 7). On the other hand, when the species were MnO 2 nanostructures, MnNPs@CALB-H 2 O presented the highest activity, being 2.2 times higher than MnNPs@CALB-DMF. Furthermore, these catalysts were evaluated in other reaction media such as 5 mM tris base buffer pH 9 and 5 mM MES buffer pH 5 ( Figure S4). In these cases, the negative effect generated by the presence of ionic strength could be observed, as in the case of copper mentioned above, where the activity in both catalysts at a pH similar to that of distilled water, such as that of the MES buffer, was 45-fold lower for MnNPs@CALB-H 2 O and 4.5-fold lower for MnNPs@CALB-DMF. In the case of a buffer with a higher pH (pH 9), for MnNPs@CALB-DMF it presented similar activity, while for MnNPs@CALB-H 2 O it was half ( Figure S4).
Finally, the MnNPs@CALB-H 2 O biohybrid exhibited activity three times higher than that of natural catalase at RT in distilled water (Figure 7).
Finally, two biohybrids of precious metals (Ag and Pd) were synthesized using bovine catalase as a scaffold (AgNPs@CATb and PdNPs@CATb) to evaluate a potential synergistic effect between catalase and metal nanoparticles. However, the specific activity of both catalysts was very low (more than 100 times lower than the Mn hybrid) (data not shown). These could be due to the type of metal species formed in each case, namely, PdCl 2 for the Pd hybrid, and a mixture of different silver oxides for the Ag hybrid.
In the literature, there are different methodologies for the degradation of H 2 O 2 as a mimetic of catalase activity. Some of them use complexes of precious metals, in some cases supported, or tubular structures, where it is necessary to synthesize systems under drastic and toxic conditions [27][28][29][30]. Therefore, the methodology proposed in our work has an advantage regarding simplicity and sustainability in the creation of artificial metalloenzymes capable of degrading H 2 O 2 in aqueous medium at RT.

Conclusions
In conclusion, in this study we have presented a simple but versatile strategy for preparation of different catalase-like activity artificial metalloenzymes (MeNPs@Enzyme) based on the application of an enzyme that induces the in situ formation of metal nanoparticles on the protein network.
Different bionanohybrids of different metals (Cu, Mn, Pd, Ag) were synthesized and evaluated in terms of their ability to decompose hydrogen peroxide at RT in aqueous media. MnNPs@CALB-H 2 O, formed by MnO 2 nanostructures, presented excellent mimetic activity, being even three times higher than natural catalase. Therefore, these results open the potential application of these novel bionanohybrids in combination with other enzymatic systems for biological and biomedical processes.