Unravelling the Potency of Activated Carbon Powder Derived from Cultivated Marine Microalgae as a Promising Filler in Mixed Matrix Membranes

Activated carbon-filled mixed matrix membranes were commonly used to enhance the separation performance of liquid or gas separation processes. Activated carbon is traditionally derived from agricultural crops such as coconut shells or wood biomass. Marine microalgae however have a great potential to produce powdered activated carbon. In this study, marine microalgae Chlorella vulgaris have been evaluated for their carbon content, and the 16.09% carbon content has potential to be employed as a raw material in manufacturing activated carbon powder. Dry microalgae were carbonized at a temperature of 500 ◦C for 30 min, at a constant increment rate of temperature of 10 ◦C per minute to produce microalgae charcoal. Chemically-based activation treatments using H3PO4 and ZnCl2 with concentrations of 10%, 30%, and 50%, respectively, assisted by microwave irradiation, have been used to prepare activated carbon. The properties of activated carbon powder were analyzed including yields, ash content, volatile substances, pure activated carbon content, absorption of iodine solution, surface area, and imaging of activated carbon using SEM-EDX. The best treatment characteristics were obtained using H3PO4 at a concentration of 50% with characteristics of 19.47% yield, 11.19% ash content, 31.92% volatile content, 56.89% pure activated carbon, 325.17 mg g−1 iodine absorption, and 109.273 m2 g−1 surface area based on the Brunauer–Emmett–Teller (BET) method, as well as a 5.5-nm average pore diameter. The SEM-EDX imaging results showed the formation of micropores on the surface of activated carbon, with carbon content reaching 72.31%; however, impurities could decrease the surface area and reduce the absorption performance of microalgae activated carbon.


Introduction
Biomass, especially from agricultural and aquacultural products, has become an important source for manufacturing biofuels, bioproducts, and biomaterials were are used for various industrial purposes [1][2][3].Microalgae, among others, have received much attention because these microscopic photosynthetic organisms are promising sources of biomass and have several advantages over land-based crops [4].Microalgae produced higher yields of lipids as raw material for biodiesel [5].Microalgae are the fastest growing photosynthetic organisms.Besides consuming CO 2 compounds and N elements from the atmosphere, microalgae are also one of the most important producers of oxygen on Earth [6].When compared with terrestrial plants, microalgae have higher photosynthetic activity, faster growth rates, and better CO 2 fixation efficiency, which is around 10-50-times [7].Microalgae contain almost 50% of carbon on a dry weight basis, and theoretically, every 1 kg of dry algal biomass can absorb about 1.83 kg CO 2 [8].
Referring to the microalgae biomass potential, which contains many important elements and can be cultivated rapidly, microalgae become an important source of food ingredients, health care products, biofuels, animal or aquaculture feeds, pigments, and cosmetics [9,10].Moreover, recent studies concerning the utilization of microalgae biomass also focused on the utilization of microalgae biomass for biohydrogen production, biogas, bio-crude, and biochar by fermentation, anaerobic processes, liquefaction, and pyrolysis [11][12][13][14][15][16].Yet, there is a great potential of microalgae that is not widely evaluated, which is the use of microalgae as an activated carbon source.Research on the production of microalgae-based activated carbon is barely found in the literature, despite its immense potential.
Activated carbon is a porous carbon material, commonly used as an absorber for various industrial purposes, from chemicals production, to automotive manufacture, to food industries.In separation processes, activated carbon could be used to enhance membrane separation performance.Membrane filtration is effectively replacing conventional, complicated, and costly separation processes.However, membrane processes also possess serious hindrances such as fouling [17,18].Activated carbon impregnated in membrane materials could improve mechanical strength, enhance rejection, and minimize fouling [19].The aforementioned concept is commonly defined as composite or mixed matrix membranes [20].As a membrane filler, activated carbon promoted the enhancement of the membrane surface hydrophilicity in a polyethersulfone (PES) nanofiltration membrane for sulfate and copper removal [21] and a nanofiber polyvinylidene fluoride-co-hexafluoropropylene (PVDF-HFP) membrane for direct contact membrane distillation [22].Activated carbon-embedded membranes also helped with reducing fouling in polyvinylidene fluoride (PVDF) ultrafiltration for water treatment [23] and for treatment of dairy effluent [24].Not only applicable to liquid separation, but a mixed matrix membrane with activated carbon filler also improved the selectivity of CO 2 /CH 4 separation [25], C 3 H 8 /CH 4 separation [26], CO 2 /CH 4 , H 2 /CH 4 , O 2 /N 2 separation [27], as well as fuel desulfuration by pervaporation [28].
Obviously, activated carbon enhances membrane filtration performance.The biomass source of activated carbon, however, is still limited to terrestrial crop biomass, such as coconut shell, palm shell, wood, peat, and even bituminous coal [29].Therefore, the evaluation of activated carbon derived from non-crop biomass is essential.This study evaluated the synthesis of activated carbon from microalgae Chlorella vulgaris biomass.Following the carbonization stage, chemical activation methods assisted by microwave irradiation were used to optimize the formation of activated carbon pores with a shorter time compared to activation using conventional heating [30].This study is aimed at the utilization of microalgae-based activated carbon powder to improve separation performance of polymer membrane, especially for liquid and gas separation.

Materials
Microalgae Chlorella vulgaris used as raw material for activated carbon synthesis in this study was cultivated and harvested at the Brackish Aquaculture and Aquaculture Center, Situbondo, Indonesia.Microalgae biomass was cultivated for 7 days in an open pond with the addition of Walne fertilizer with a mixture of KNO 3 , NaH 2 PO 4 , Na 2 EDTA, and FeCl 3 to provide adequate nutrients for microalgae growth.Following the harvesting stage using the flocculation method, microalgae biomass was dried using an oven at 60 • C for 8 h, and the drying continued with sunlight to obtain microalgae with a 4.47% moisture content for Chlorella vulgaris powder.Carbonization of dry microalgae biomass was carried out at 500 • C with a heating rate of 10 • C.min −1 and a holding time of 30 min.Microalgae charcoal size produced from the carbonization process was equal to 149 µm as measured by a wire mesh.Then, 8 g of microalgae charcoal samples were immersed in 30 mL of H 3 PO 4 and ZnCl 2 solution with concentrations of 10%, 30%, and 50%, respectively.Soaking was carried out for 24 h, followed by 600-W microwave irradiation for 8 min (equivalent to an estimated temperature of 150-180 • C).
Following the activation process, the activated carbon produced was cleaned by washing using 1 M heated HCl and subsequently washed using distilled water until the pH of the activated carbon was nearly 7.0.The washed activated carbon obtained was then dried using an oven at 105 • C for 24 h until dry activated carbon was obtained.Each series of experiments in the study was repeated three times.The evaluated parameters included ash content, volatile substances, pure activated carbon content, iodine solution absorption, surface area, pore structure, and element composition.

Ash Content
The ash content measurement was done according to the standard ASTM D 2866-70.Samples of activated carbon of 1 g were taken from each treatment, then put into a porcelain crucible.Samples and the porcelain crucible were put into a furnace with a temperature of 750 • C for 1 h.The porcelain crucible was taken and cooled, then the final sample mass was weighed.The heating process with a furnace was repeated until a constant ash weight was obtained.The value of ash content was calculated by Equation (1) as follows: where A c is the ash content (%); m 0 is the initial mass (g); m 1 is the final mass (g).

Volatile Matter Content
The volatile matter content measurement was done based on ISO 562-1981.Samples of activated carbon of 1 g were taken from each treatment, then put into a porcelain crucible.Samples and the porcelain crucible were heated at 950 • C for 10 min using a furnace.The porcelain crucible was taken and cooled, then the final mass of the sample was weighed.The value of the vaporized substance was calculated using Equation (2) as follows: where V D is volatile matter content (%); a is the initial mass of the sample before heating (g); b is the final sample mass after heating (g).

Pure Activated Carbon Content
The calculation of bound carbon levels or levels of pure activated carbon was the amount of carbon resulting from the combustion process without the presence of other substances contained in carbon, such as ash and other substances that are still attached to the surface of activated carbon.Pure activated carbon levels can be calculated using Equation (3) as follows: where C PAC is pure activated carbon content (%).

Iodine Absorption
The iodine absorption measurement were done based on American Water Works Association standard method AWWA B 600-78.Samples of activated carbon of 0.25 g were weighed and then put into an Erlenmeyer tube.A standard Iodine solution of 0.1 N was added to 25 mL.The mixture was stirred for 15 min, then filtered.As much as 10 mL of this filtrate were put into another Erlenmeyer tube, then the filtrate was titrated with 0.1 N sodium thiosulfate until turning pale yellow.An indicator of 1% starch solution was added, and the titration continued until the filtrate became clear.The amount of sodium thiosulfate solution used must be recorded, to calculate the value of iodine absorption, by using Equation (4) below: where IAN is the iodine number (mg/g); B is the volume of the total sodium thiosulfate used during titration (mL); C is the normality of sodium thiosulfate (N); D is the normality of iodine (N); W is the mass of activated carbon (g); 12.693 is the amount of iodine that corresponds to 1 mL of 0.1 N sodium thiosulfate solution.

Surface Area
Surface area was measured based on the Brunauer-Emmett-Teller (BET) method by using the Nova 1200e Surface Area Analyzer (Quantachrome Instruments).External or non-microporous area and micropore volume (Vp) were calculated based on nitrogen gas adsorption.Before measuring its surface area, the sample mass was weighed first so that the surface area could be known every 1 g of sample.Details of the measurement were described elsewhere [31].

Pore Imaging and Elemental Composition
Investigation of the morphology of activated carbon and elemental composition was carried out using the SEM-EDX Inspect 24 (FEI Ltd., Hillsboro, OR, USA).SEM testing was carried out to determine the surface and pore patterns of activated carbon formed, while EDX testing was carried out to analyze the composition of activated carbon elements.Surface functional groups of activated carbon were analyzed using an FTIR spectrophotometer (8400S/Shimadzu, Kyoto, Japan).

Characteristics of Biomass Microalgae Chlorella vulgaris Raw
The composition of raw materials is an important factor in the manufacture of activated carbon, because it can affect the pore arrangement and the characteristics of the activated carbon produced.As shown in Table 1, the biomass composition of Chlorella vulgaris microalgae consisted of various elements; the presence of carbon content detected in the Chlorella vulgaris biomass indicates that this biomass has the potential to be used as raw material for activated carbon even though the amount was relatively small.Other elements were also detected in the microalgae Chlorella vulgaris sample, namely Na and Cl elements; these elements are a salt-forming element, which is generally contained in brackish water used as a medium for the cultivation of microalgae.In addition, the presence of other elements contained in microalgae biomass in small amounts such as Mg, P, S, Ca, and Si are thought to originate from the standard fertilizer composition used to nourish the growth of microalgae containing KNO 3 , NaH 2 PO 4 , Na 2 EDTA, and FeCl 3 and direct contact between microalgae culture media with the external environment, because microalgae were developed using the open pond method, not by using a photobioreactor, so that contamination from the outside environment was possible.

Characteristics of Microalgae Chlorella vulgaris Charcoal
Charcoal produced from the carbonate process was dark black, had a brittle structure, did not smell, and did not dissolve in water.The yield obtained from the carbonization process of dried microalgae powder to become microalgae charcoal was 57.27%, indicating that carbonization had a large influence on the yield of charcoal obtained.This condition was due to some volatile substances disappearing along with the carbonization process.Further oxidation also occurred optimally without the absence of an inert gas that functions as an inhibitor of the oxidation reaction during the carbonization process.Pyrolysis of biomass Chlorella vulgaris will undergo three stages: heating at room temperature up to 180 • C; at this stage, steam will be released from the carbon matrix and will experience a mass loss of 6.87%.In the second stage at a temperature of 185-596 • C, devolatilization and pyrolysis occurred; at this stage, the weight loss was clearly seen compared to the first stage, and the reaction was obtained by increasing the speed with the maximum temperature as 307 • C; at this stage, the biomass will lose 55%.In the third stage with a temperature of 600-800 • C, solid charcoal material occurred slowly with constant degradation after the devolatilization process was complete [32].
The results of SEM-EDX testing showed that the elemental components contained in the microalgae charcoal were not much different from the components of the microalgae dry powder, as shown in Table 1, which still contained elements of C, O, Na, Mg, Cl, and other elements in very small amounts.The existence of this carbonization process caused an increase in the average weight of carbon elements in carbonized charcoal compared to dry microalgae powder, which rose to 27.81% from the original 16.09%.The increase in elemental weight also occurred in elements O, Mg, Ca, and Zr; the increase in these element indicates that the elements did not undergo decomposition during the carbonization process; on the other hand, if there was a decrease in the weight of elements such as Na, P, Cl, and Ca, this would show that the element decomposed during the carbonization process.Materials containing cellulose, hemicellulose, and lignin in the carbonization process using 500 • C temperatures can decompose and produce various products including tar, water vapor, carbon, and some others, such as CO, CO 2 , CH 2 , and H 2 gases.

Activated Carbon Yield
The yield of the carbonization process was generally charcoal with porosity and small surface area because it was still covered with hydrocarbon deposits.It had a less effective absorption capacity; therefore, the charcoal obtained after the carbonization process will be upgraded through the activation process.Among the important parameters during the active transfer of microalgae charcoal until the activated charcoal was activated charcoal yield.As shown in Figure 1, the yield value obtained from the largest was 19.28-66.51%.The lowest yield was obtained by using an H 3 PO 4 activator agent with a composition of 50% and the highest yield obtained by using a ZnCl 2 activator agent with a composition of 50%.The use of H 3 PO 4 activator agents with increasing concentration tended to reduce the yield of activated carbon produced; this is because the composition possessed by microalgae allows for a more effective reaction with acid.In contrast to the use of activating agent ZnCl 2 , it was found that the increasing concentration of activating agents tended to increase the yield produced.This is due to the composition possessed by microalgae charcoal, which does not react effectively with activating agent ZnCl 2 , so that it still leaves Zn and Cl residue on activated carbon, which also gives a mass increase in microalgae activated carbon.

Ash Content
Analysis of ash content in activated carbon aimed to determine the content of metal oxides in activated carbon.The ash content was indicated as mineral residue that was still left behind during the carbonization process, because the microalgae biomass used as the raw material for activated carbon did not only contain carbon, but also mineral elements.Some minerals were lost during the carbonization and chemical activation process, but others were thought to remain in the activated carbon component.The ash content of activated charcoal was very influential on the activated carbon obtained.The presence of ash as a result of oxidation volatile components contained in activated carbon can cause clogging of activated carbon pores and will affect the surface area of activated carbon.As shown in Figure 2, the ash content from activation using H 3 PO 4 activator agent ranged from 12.98-38.44%,and activation using ZnCl 2 ranged from 47.38-48.79%.The highest ash content was obtained by using ZnCl 2 activating agent at a concentration of 50%, and the lowest ash content was obtained by treatment using the H 3 PO 4 activating agent at a concentration of 30%.Moreover, the value of the activated carbon ash content results of activation were still smaller than the non-treatment sample activation, which reached a value of 56.87%.
From the results obtained, it was shown that the ZnCl 2 activator agent produced more ash content compared to using acid activator.This condition occurred because during the activation process, the components other than carbon contained in the microalgae charcoal reacted with activating agents used in this study, thus reducing the ash content of activated carbon, where ash content was accumulated mineral salts and metal elements that were still found in microalgae charcoal after the carbonization process.The ash content, which was relatively large, was thought to be due to the initial composition of the raw material and direct contact with the air, so that the carbonization process did not run perfectly, resulting in the formation of larger amounts of ash.The ash content of the activated carbon was produced from the oxidation reaction, which caused the adsorption to decrease, because the activated charcoal pore was covered by various minerals such as Na, Ca, K, and Mg, which were still attached to the surface of the activated charcoal.In a study conducted by [33], it was also known that the largest components found in activated carbon ash were Ca, Al, Si, Fe, and S.

Volatile Matter Content
The levels of volatile matter content in activated carbon indicated evaporation of elements and non-carbon compounds.The level of substances that evaporated was the result of decomposition of constituent elements of activated carbon due to the heating process during the carbonization and activation process.Figure 3 shows the mean levels of the volatile matter of activated carbon of microalgae Chlorella vulgaris.The level of the vaporized substance of activated carbon as a result of activation using the H 3 PO 4 activator agent ranged from 27.10-30.36%,and activation using ZnCl 2 ranged from 19.79-23.21%.The highest levels of volatile substances were obtained by treatment using the H 3 PO 4 activator agent at a concentration of 30%, and the lowest levels of the evaporated substance were obtained by using the ZnCl 2 activator agent at a concentration of 10%.Vapor content values obtained from all treatments were generally greater than those of microalgae without activation treatment because after the activation process, micropores formed on the surface of activated carbon, and the resulting micropore was occupied by volatiles, which made the level of volatile substances from activated carbon activation results increasingly increase.

Pure Activated Carbon Content
The level of pure activated carbon was carbon that bound in the activated carbon other than the volatile substance fraction and ash content.Figure 4 shows the average levels of pure activated carbon of microalgae Chlorella vulgaris.The level of pure activated carbon from activation using the H 3 PO 4 activating agent ranged from 34.46-56.66%,and activation using ZnCl 2 ranged from 27.99-32.24%.The highest levels of pure activated carbon were obtained by treatment using the H 3 PO 4 activator agent at a concentration of 50%, and the lowest level of pure activated carbon was obtained by treatment using activator agent ZnCl 2 at a concentration of 30%.From the data obtained, it was known that in general, the activation carried out was able to increase the content of pure activated carbon compared with microalgae charcoal without treatment, except in the activation process using ZnCl 2 at concentrations of 30 and 50% obtained by the level of smaller pure activated carbon due to the content ZnCl 2 , which covered the pores of activated carbon.The content of pure activated carbon was influenced by the ash content, the level of the substance vaporized, and the presence of hydrocarbon compounds that were still attached to the activated carbon surface.The high level of bound carbon showed that the carbon fraction bound in activated carbon resulting from the activation process was getting higher.

Iodine Absorption
The iodine absorption test aimed to determine the ability of activated carbon to absorb colored solutions with a molecular size of less than 10 Å or 1 nm. Figure 5 shows the average absorption of iodine by activated carbon microalgae Chlorella vulgaris.Absorption of the iodine solution of activated carbon as a result of activation using the H 3 PO 4 activating agent ranged from 256.15-330.39mg g −1 , and activation using ZnCl 2 ranged from 192.09-230.31mg g −1 .The activation that had been done was able to increase the absorption of microalgae activated carbon against iodine solution.The results of the largest absorption of iodine solution were obtained by treatment using the H 3 PO 4 activator agent at a concentration of 50%.
High and low of iodine absorption refers to the number of micropores formed during the activation process.The greater iodine absorption indicates a greater number of micropores formed, or in other words, a greater surface of the activated carbon produced.The active absorption of charcoal was generally influenced by the nature of activated charcoal, the nature of the solution or components to be absorbed, and the contact system of the activated charcoal surface.The low absorption of iodine was generally caused by damage to or erosion of the pore surface of activated carbon, but the low absorption of iodine in this study was thought to be due to the large amount of impurities remaining after activation, which cover the pores of activated carbon and affect the ability of activated carbon to absorb iodine.

FTIR Analysis
The FTIR spectra of non-activated Chlorella vulgaris dry raw mass powder and activated carbon prepared from 50% H 3 PO 4 and 10% ZnCl 2 are presented in the following Figure 6.From the spectra obtained, it was known that biomass Chlorella vulgaris had a more complex bond than the activated carbon microalgae Chlorella vulgaris with activation of 50% H 3 PO 4 and 10% ZnCl 2 .It is known that the activation process is able to decompose the complex bond in dry microalgae raw materials.The complete compounds of Chlorella vulgaris dry raw mass powder and activated carbon prepared from 50% H 3 PO 4 and 10% ZnCl 2 are listed in Table 2.The results revealed that a large amount of chemical functional groups C≡C, C=C, =CH, ≡CH, C-O, C=O and -COOH were preserved and generated on the surface of activated carbon during the activation stage, which might enhance its adsorptive properties [34].In a study conducted by [33], it was known that wavelengths between 1200 and 400 cm −1 in the FTIR spectrum are activated carbon ash, which indicate most mineral components.
3.9.Surface Area and Pore Properties BET surface area testing was carried out on the sample with the best iodine absorption from each activator agent given, namely the ZnCl 2 10% and H 3 PO 4 50% treatment.Based on the N 2 adsorption-desorption isotherm as shown in Figure 7, it can be known in general for activated carbon products that have a size smaller than 20 Å, which indicates that micropores were present in this adsorbent.The form of the isotherm in the activated product also showed a significant increase in the adsorption process at relative pressure because of the interaction of the high adsorbates, then reached the peak of the compilation, which showed the relative increase in activated carbon, which was fully filled.This type of isotherm is suitable for micropore adsorbents with pores no wider than 20 Å.This finding is consistent with the high ratio of the micropore volume of the micropore area and the BET surface area and total pore volume [35].The pore size of activated carbon was obtained using the BJH method using the carbon pore volume in the measurement of the N 2 desorption isotherm.From the results of testing using the surface area analyzer, it was known that the BET surface area of the two sample samples was 115.352 m 2 g −1 and 109.273m 2 g −1 , respectively; while the average pore diameter was 1.985 Å and 0.55 Å, respectively.The largest surface area will be obtained when the pore diameter is smaller in size and they are greater in number.The depiction of the average pore diameter with the pore surface area and pore volume formed is shown in Figures 8 and 9.

Pore Structure and Elemental Composition
The pore structure is very closely related to surface area, where the more active carbon pores will have an impact on the surface area of activated carbon and the greater the speed of adsorption.SEM imaging was focused on the sample with the highest iodine absorption from each activator agent, namely activated carbon from activation with ZnCl 2 10% and 50% H 3 PO 4 compared with dry microalgae powder as raw material, microalgae charcoal, and commercial activated carbon.The pore structure results of imaging using SEM are presented in Figure 10.
Figure 10 shows that the raw material of dry microalgae powder and charcoal from carbonization results showed different shapes due to decomposition during the carbonization process using a temperature of 500 • C, but on the surface, neither showed micropore formation.In contrast to activated charcoal that has gone through the activation process, in Figure 10b-d have addressed the formation of micropores, and this shows that the activation process using ZnCl 2 and H 3 PO 4 activator agents was able to create microporous surfaces of almost a uniform size so they can expand its surface.
As shown in Table 1, there was a change in the elemental composition of each step carried out: the carbonization process had increased the carbon, but also still contained elements in addition to carbon in sufficient quantities.The carbon results of activation using ZnCl 2 showed a smaller carbon yield compared to the charcoal from activation, and this is presumably because the content of elements other than carbon remaining after the activation process covered the carbon surface, resulting in fewer readings of carbon elements in SEM-EDX analysis.The highest carbon content was obtained from the treatment using the H 3 PO 4 activator agent with an elemental weight of 72.31%.On activation by using H 3 PO 4 , Na, P, Cl, Ca, and Zr elements were found to decrease in number or even disappear; thus, it was known that acid activators were more able to react with microalgae charcoal.If this is related to the discussion of iodine absorption, it can be known that the absorption of iodine produced in this study was relatively lower compared to commercial activated carbon because the activated carbon surface was activated by an element other than carbon, so that the absorption capacity decreased.The results of this study are comparable to the reported results in [36], which for making activated carbon using chemical activation, a good activator used for lignocellulose material was to use an acid-type activator.This is because the lignocellulose material has a high oxygen content and causes the acid activator to react with a functional group containing oxygen, while the base activator tends to react with carbon, making it suitable for high carbon-containing materials.

Future Direction of Research
Although this study presented the potential of the production of activated carbon from microalgae, especially from Chlorella vulgaris, further studies are required.Thermal analysis of TG-DTG is needed to quantify the decomposition of biomass during heating.The analysis will give more insight into the elemental composition of activated carbon.Ash content for instance could be reduced when the optimum carbonization condition is known properly.Further application of activated carbon produced in this study, with hybridization in membrane materials, is also important to be done.By embedding the activated carbon as a filler in mixed matrix membrane, the real potency of microalgae-based activated carbon could be revealed more clearly.

Conclusions
Activation using H 3 PO 4 and ZnCl 2 with different concentrations accompanied by microwave irradiation had an effect on ash content, vaporized substance level, and pure activated carbon content of microalgae Chlorella vulgaris.Activation carried out was able to reduce the content of ash compared to microalgae charcoal without treatment, and the level of volatile substances tended to be higher because the pores of carbon were occupied by many volatile substances, while carbon content was generally higher than the charcoal without treatment, with the best results using acid activator.Activation using different H 3 PO 4 , and ZnCl 2 with different concentrations was able to increase the absorption capacity of the iodine solution and enlarge the surface area due to micropore formation.Chlorella vulgaris microalgae can be used as a potential source for making activated carbon, with activation using 50% H 3 PO 4 and with microwave irradiation capable of increasing the carbon content to reach 72.31%.Moreover, the powder activated carbon could be utilized as an absorber filling in a mixed matrix membrane for gas or liquid separation.

Figure 1 .
Figure 1.The yield of microalgae Chlorella vulgaris activated carbon.

Figure 3 .
Figure 3. Volatile matter content of microalgae Chlorella vulgaris activated carbon.

Figure 4 .
Figure 4. Pure activated carbon content of microalgae Chlorella vulgaris activated carbon.

Figure 8 .
Figure 8. Pore diameter and pore surface area distribution of microalgae-based activated carbons.

Figure 9 .
Figure 9. Pore diameter and pore volume distribution of microalgae-based activated carbons.

Table 1 .
Comparison of the elemental composition of microalgae Chlorella vulgaris dry raw mass powder, charcoal, and activated carbon.

Table 2 .
Comparison of the elemental composition of microalgae Chlorella vulgaris dry raw mass powder (non-activated) and activated carbon prepared from 50% H 3 PO 4 and 10% ZnCl 2 .