Lomefloxacin—Occurrence in the German River Erft, Its Photo-Induced Elimination, and Assessment of Ecotoxicity

Pharmaceuticals in waters represent a worldwide problem of today. Advanced oxidation processes (AOPs) are being researched for elimination of the ecological hazard. Among the substances, the fluoroquinolone antibiotic lomefloxacin was selected for investigation in this study. Lomefloxacin (LOM) was found in the German river Erft. Near and far ultraviolet (UVA, UVC) radiation were used as AOPs and compared for efficiency depending on pH, water matrix, and catalysts. Chemical kinetics description revealed that UVC at pH 8–9 led to the fastest degradation of LOM. The catalysts hydrogen peroxide and titanium dioxide had only limited influence on the degradation rate. Seven novel transformation products were structurally identified by high-resolution higher-order mass spectrometry. Ecotoxicity of the novel and known compounds was assessed by quantitative structure-activity relationship (QSAR) analysis. In addition, irradiation time dependent minimal, and half-maximal inhibitory concentrations (MIC, IC50) of LOM solutions were determined and suggested as ecotoxicological hazard indicators. From MIC and kinetic rate constants, the irradiation time required for compound and activity removal could be predicted.


Introduction
Numerous recent studies have confirmed the presence of various anthropogenic micropollutants in water bodies [1][2][3]. These micropollutants include pharmaceuticals, personal care products, steroid hormones, and pesticides. The main entry route focuses on wastewater treatment plants (WWTPs), which often fail to completely eliminate these substances through the conventional purification processes. Hence, they enter the aqueous environment where they become ecotoxicologically hazardous. The regular observation of micropollutants during environmental monitoring is often associated with an increasing bacterial resistance formation [1][2][3][4]. Observed concentrations range from a few ng L −1 to several μg L −1 . Among the pharmaceuticals, fluoroquinolones are the third largest group of antibiotics in worldwide sales administered to humans and animals and are excreted without metabolization [4,5]. Nakata et al. (2005) reported fluoroquinolone concentrations up to 49 ng L −1 both in wastewater treatment effluents and in surface waters and 19 ng L −1 in river water [6].
Advanced purification stages for the elimination of these anthropogenic micropollutants are currently being tested worldwide, including chemical catalysts and UV irradiation. Some of these methods, such as UV irradiation, produce OH radicals, which act as strong oxidants with an oxidation potential of 2.8 V [7]. Several fluoroquinolones follow a degradation process according to pseudo-first order kinetics due to OH radical formation [8]. As a disadvantage, these types of advanced oxidation processes (AOPs) may produce degradation or transformation products (TPs) of potentially higher toxicity than the educt. Thus, recent research on the elimination of pharmaceuticals by AOPs extends to toxicity assessment. As suitable measures, quantitative structure-activity relation (QSAR) analysis as well as minimum inhibitory concentration (MIC) and half maximum inhibitory concentration (IC50) have been discussed [9][10][11]. Both methods were compared in this study.
The fluoroquinolone lomefloxacin served as a model compound since this substance was found in various waters in previous studies and also in the nearby River Erft in the course of this study [12,13]. Its irradiation-induced transformation has been described earlier [14,15]. Yet, systematic comparative investigation of near and far ultraviolet (UVA and UVC) irradiation in the presence and absence of hydrogen peroxide and titanium dioxide has not been undertaken. Both reagents were chosen due to their accelerating effect on degradation [16,17]. Time-dependent degradation and/or transformation of educt and known and novel transformation products were monitored using highperformance liquid chromatography and high-resolution mass spectrometry. In parallel, total organic carbon (TOC) was monitored for chemical effectiveness. To consider various aquatic conditions, model water at different pH values, river water, and effluent were used as matrices. As transformation products have often been assumed more ecotoxic than their initial drug substance, irradiation-time dependent MIC and/ or IC50 and QSAR analyses were performed to assess the ecotoxicological hazard [18][19][20]. Mostly, these tools were used to determine the toxicity of new pharmaceuticals, among them fluoroquinolones [21,22]. Only one study could be found concerning photodegradation of fluoroquinolones in combination with QSAR [23]. Based on MIC and chemical kinetics, irradiation times depending on aquatic conditions could be predicted.
Surface water samples were taken from the German river Erft (Neuss) in summer 2018 downstream of a WWTP and from the river Rhein near Krefeld Uerdingen, see Figure 1. Both samples were checked for the presence of LOM using solid-phase extraction and analyzed with HPLC-ESI-Q-TOF-MS.

Solid-Phase Extraction of River Water Samples
Using solid-phase extraction (SPE), 1 L of a filtered water sample was concentrated to 1 mL. Oasis HLB (Waters, Milford, CT, USA) SPE cartridges were first conditioned with 3 mL of methanol (Merck, Darmstadt, Germany), quenched with 3 mL of MilliQ water, loaded with 1 L of river water, washed with 3 mL of MillliQ water, and then eluted with 3 mL methanol. The substances dissolved in methanol were concentrated to dryness using the rotary evaporator and then taken up again with 1 mL MilliQ water. The sample was cleaned with a syringe filter and then transferred to the HPLC-MS system.

Photodegradation Experiments
Photoinduced degradation experiments were carried out in a 1 L batch reactor (Peschl Ultraviolet GmbH, Mainz, Germany). For irradiation, a UVA light emitting medium pressure mercury lamp (Heraeus, TQ 150, 150 W, Hanau, Germany) was operated with cooling to prevent power associated temperature increase. For UVC irradiation a low-pressure mercury lamp (Heraeus TNN 15/32, 15 W, Hanau, Germany) was operated without need for cooling. The reaction temperature throughout the reactor was 22 ± 2 °C, checked during degradation by means of a thermometer. The lamps were arranged centrically in the reactor. The reactor was filled with 750 and 800 mL aqueous solution with the UVA and UVC lamp inserted. The concentration of LOM was 20 mg L −1 . The pH was adjusted by adding hydrochloric acid (HCl, 30% Suprapur, Merck KGaA, Darmstadt, Germany) or ammoniacal solution (NH3, approximately 25% Riedel-de Haen, Seelze, Germany). A magnetic stirrer (500 rpm) was used. Irradiation times were 30 min and 10 min for UVA and UVC irradiation, respectively. In previous studies, this exposure time has proven to ensure sufficient degradation [11,[24][25][26].
The warm-up time of the UVA lamp was 2 min vs. 0 min for the UVC lamp. Both lamps emitted polychromatic light with maximum intensities at 313, 365, 405, 437, 547, 578, and 580 nm. The UVC lamp additionally emitted at 185 and 254 nm. The total photon flux in the range between 200 and 500 nm was determined to 3.50 mmol•min −1 •L −1 for the UVA lamp and 2.03 mmol•min −1 •L −1 for the UVC lamp by means of ferrioxalate actinometry according to IUPAC [27,28].
The occurrence of OH radicals on irradiation of water was proven by Electron Spin Resonance (ESR) spectroscopy using spin traps (data not shown) following Sun et al. (1996) and Kochany et al. (1991Kochany et al. ( , 1992 [29][30][31]. The photocatalytic degradation of LOM was also carried out in the presence of hydrogen peroxide (30% stabilized, Carl Roth, Karlsruhe, Germany) and titanium dioxide (P25 Aeroxide ® , Arcos Organics, Geel, Belgium). Hydrogen peroxide was added to the solutions leading to final concentrations of 10 mg L −1 and 30 mg L −1 in the mixture. Concentrations were determined with Merckoquant test strips before and after irradiation. Further, titanium dioxide was used as a photocatalyst. Addition prior to irradiation led to concentrations of 50 and 100 mg L −1 titanium dioxide. The resulting milky solutions were irradiated with UV light.
In addition, degradation experiments were carried out with the effluent of a local WWTP (Krefeld) and from the river Erft (Neuss). The color of the wastewater was yellowish in both cases, the pH values of the two samples were determined to 8. Each sample was spiked with 20 mg L −1 LOM. This concentration was chosen to obtain results comparable with those of LOM in MilliQ water.

HPLC-ESI-Q-TOF-MS and HPLC-ESI-IT-MS
For the identification of transformation products at high sensitivity and high reliability, mass spectrometry was used as a detection system after chromatographic separation. Here, time-of-flight (TOF) MS was used for highly accurate m/z determination. Multiple fragmentation for structure elucidation and verification was achieved through ion-trap MS. Samples were analyzed using a highperformance liquid chromatography system (HPLC, Agilent 1100) combined with an ion-trap mass spectrometer (IT-MS, Thermo Finnigan LXQ, Waltham, MA, USA) to yield MS n . A reversed-phase column 3-Amides C-18, 150 mm × 2.00 mm, 3 μm (Polaris Agilent) was used for chromatographic separation. Temperature was kept at 40 °C and chromatography was performed isocratically within 15 min using MilliQ water (90%) and methanol (10%) with 0.1% formic acid each (Merck KGaA, EMSURE ® ACS, Darmstadt, Germany, 98-100%) as eluents. The flow rate was set to 0.3 mL/min for all measurements. An electrospray ionization (ESI) source was attached to the IT-MS. For all experiments, the positive mode was used. The gas flow was adjusted to 15 L min −1 , the capillary temperature was set to 300 °C and the capillary voltage amounted to 47 V. The mass range was scanned from 100 to 1000 m/z. Tandem or higher MS experiments were initiated using the software's auto MS n mode using a threshold trigger. Mass spectrometer and HPLC system were controlled via XCalibur 2.0 running on a personal computer under Windows XP. Chromatograms and mass spectra were processed using the same software. Accurate masses were obtained using an ESI-Q-TOF mass spectrometer (Agilent 6530). A dual jet stream (ESI) source was used in positive mode. The collision gas flow was set to 8 L min −1 , the gas temperature was 300 °C and the fragmentor voltage 175 V. The instruments were controlled via MassHunter Workstation B.06.00 running on a personal computer under Windows 7 Professional. Data were processed using the same software.

TOC
The total organic carbon (TOC) was determined using a TOC analyzer (TOC-V CSN Series Shimadzu, Kyoto, Japan). Calibration was performed between 2 and 20 mg L −1 carbon using solutions of sucrose. The pH of all samples was adjusted to 1-3 using concentrated sulfuric acid. Subsequently, potentially resulting gases in the samples were removed by ultrasonification for 15 min. Samples were taken before irradiation and during irradiation after 15, 30, 45, and 60 min and every hour for a total of 7 h. Injection volume was 150 μL for each sample. The device was controlled and results evaluated using TOC-Control software (Shimadzu, Kyoto, Japan). Each sample was measured in triplicates. Mean values yielded the concentrations. Prior to measurements, the TOC analyzer was equilibrated with the new sample to remove residual carbon. In addition, between each LOM sample acidified and degassed MilliQ water was measured.

Kinetic Analysis and Determination of Quantum Efficiency of the Photodegradation
Kinetic analysis of degradation profiles followed first-order or pseudo first-order kinetic models, consecutive and subsequent follow-up reaction models, cf. Equation (1), according to previous studies and theory [26,[32][33][34][35][36][37]. The complete kinetic description is represented in the Supplementary Information.
Substance A reacts with the reaction rate constant k1 yielding an unknown number of products. The product can continue to react. The sequence can be understood as a consecutive or subsequent follow-up reaction. Rigorous mathematical treatment returns the time-dependent concentration c of educt A, with the actual and the initial concentration of A, and the time t, cf. Equation (2).
From mass area-under-the-curve versus irradiation time graphs, degradation curves were obtained. The software MatLab R2017a (MathWorks, Natick, MA, USA) containing the curve fitting toolbox was used to compute the kinetic profiles of the degradation intermediates and products according to Equations (2)-(4) (see also Supplementary Information).

Determination of Quantum Efficiency
The quantum efficiency may be defined as the degradation rate divided by the number of photons absorbed [29]. The quantum efficiency at 254 nm was calculated using Equation (3) where k (min −1 ) is the reaction rate constant of the degradation A→B, l is the reactor width of 3 cm, ε254 is the molar extinction coefficient in L mol −1 cm −1 and I0,254 the photon fluence rate in mmol min −1 L −1 .

Assessment of Ecotoxicology
The assay method for the determination of MIC values of LOM followed the protocol by Wiegand, Hilpert, and Hancock [39]. The assay protocol is also given in ISO 20776-1:2007. As ubiquitous bacteria, Pseudomonas fluorescens (DSM-No. 50090) and Bacillus subtilis (DSM-No. 10) were selected. Bacterial growth was monitored before and after UV irradiation [40]. For more details see Supplemental Information. The calculation of IC50 was performed on the basis of the plot of bacterial growth versus compound concentration.
The time tact according to Equation (4) represents the decline in activity an antibiotic possesses against bacteria. It can be calculated from kinetic parameters obtained from degradation experiments, i.e., the and the rate constants k and the initial compound concentration cA0, and the MIC values (see above) [36].
ln MIC The software distributions T.E.S.T. and OECD QSAR Toolbox were applied for QSAR analyses [41,42]. Within T.E.S.T, the module QSAR was selected. Compound structures were drawn using ACD/ChemSketch 2016.1.1 (ACDLabs, Inc., Toronto, ON, Canada) and imported into the software T.E.S.T and QSAR toolbox. As relevant target organisms, Daphnia magna LC50 (48 h) in mg L −1 , fathead minnow LC50 (96 h), 'Photoinduced Toxicity on D. magna', and 'Mortality LC50 (48 h) of branchiopoda' for non-specified test organisms were chosen due to their presence in the aquatic environment.
In neutral solutions, fluoroquinolones occur as zwitterions, as can be recognized from their pKa values. This property exercises influence on the photoinduced degradation through directing the chemical transformation and through the UV absorption efficiency, which depends on the pH as well, see Figure 2a. The spectral range of the irradiation also affects the quantum efficiencies. Hence, UVC or UVA lamps used in this study may lead to different transformation pathways and products. In order to predict conditions for efficient degradation of LOM, absorption spectra were recorded at different pH values and compared with the emission spectra of the lamps, see Figure 2b.

Occurrence of Lomefloxacin in Surface Water
The presence of LOM is a worldwide problem, and thus, also in Germany. A sample from the River Erft showed the occurrence of LOM, whereby no LOM was observed in the sample from the River Rhine in summer 2018. The findings were confirmed through comparison of chromatographic retention times and the MS/MS fragmentation pattern with a LOM reference sample, see Figure 3. The accurate mass observed agreed also perfectly with the theoretical monoisotopic mass, cf. Table  1. Further substances were found in the river, such as the b1 receptor blocker metoprolol. The remaining substances found in both river water samples were not considered in this study. Samples containing LOM were taken from the River Erft immediately after a sewage treatment plant, while in samples collected upstream of the plant showed no presence of LOM. In agreement with previous studies, WWTPs seem to concentrate LOM and act as entry path into the aquatic environment [43,44]. Findings as this underline the need for a fourth purification stage based on AOPs or at least advanced filtering systems. In this respect, the photo-induced degradation of LOM is investigated.

Photoinduced Degradation of Lomefloxacin-Kinetic of Degradation
The influence of UVA and UVC radiation, pH values, and model and surface water matrices on the photoinduced degradation were analyzed. Examples are given in Figure 4. Photoinduced transformation and degradation were monitored using HPLC-MS techniques and plotted as concentration or area-under-the-curve mass signals versus time (c-t). The c-t curves obtained could be best described by first or pseudo-first order kinetics in agreement with previous studies [25,26,36]. The results are presented in Table 2 as kinetic rate constants and quantum efficiencies. Radiation range, pH, and matrix were found to influence the degradation rate. While the degradation of LOM in acidic milieu was slowest, it could be quadrupled by increasing the pH. The water matrix also affects the degradation rate. In MilliQ water, the degradation is faster compared to effluent and river water. In environmental water matrices, a large variety of compounds are present and decrease the amount of light reaching LOM which induces its transformation or degradation. The effect of pH on the rate constants may be twofold. Firstly, LOM itself may be degraded faster in its zwitterionic or anionic state, like in neutral and alkaline milieu. Secondly, the photoinduced formation of hydroxyl radicals contributes to the degradation and is pH dependent. The presence of hydroxide hampers hydroxyl radical induced degradation, hence would decrease the part of the rate constant originating from this mechanism. Yet, hydroxide can act as a reagent itself and would lead to an increase of the rate constant. As can be seen from Table 2, quantum efficiencies exceeding 1 suggest more than one mechanism in action. In conclusion, the obviously best conditions for the removal of LOM were UVC irradiation and a pH between neutral and slightly alkaline.
Among AOPs, photocatalysts, such as titanium dioxide in combination with UVA irradiation or hydrogen peroxide in combination with UVC irradiation, were often used to accelerate the degradation of many anthropogenic micropollutants [7,[45][46][47]]. Yet, no significant differences were observed between presence and absence of these photocatalysts in this study, see Table 3. The sole exception occurred when the degradation was accelerated in the presence of hydrogen peroxide under UVC irradiation at pH 3-4. The lack of efficiency of hydrogen peroxide was traced back to saturation effects [7,48], while the presence of titanium dioxide was assumed to cause light scattering hence reducing the amount of light for direct degradation [49]. Since effluents most often show pH values in the neutral or weak alkaline range, no need for the application of hydrogen peroxide or titanium dioxide was indicated. To this point, only the induced degradation of LOM was considered. Since organic decomposition products were formed, it was also interesting to monitor TOC with irradiation time. Figure 5 shows the irradiation time dependent TOC of LOM in river Erft water and MilliQ water at an initial concentration of 20 mg L −1 and under UVC irradiation. The initial TOC in river water was about 6.5 times higher than that of MilliQ water containing 20 mg L −1 of LOM, since the river water contained a variety of organic substances. After 7 h of UVC irradiation, the TOC in both samples was reduced by 10 mg L −1 . As the initial TOC of the MilliQ water sample amounted to 10 mg L −1 , complete mineralization was achieved. In contrast, the irradiation time of the river Erft water sample would have had to be significantly extended for complete mineralization. It is obvious that TOC is no suitable measure for the removal of LOM or other pharmaceuticals as surface waters or effluents carry innumerable organic compounds that react differently to UV exposure. Nevertheless, it is reasonable to assume that LOM degradation proceeded via transformation products that were analyzed and identified using HPLC-ESI-Q-TOF-MS and HPLC-ESI-IT-MS as described in the following section.

Photoinduced Degradation Products of Lomefloxacin
During the degradation experiments, both known and unknown compounds were identified by HPLC-ESI-IT-MS and/or HPLC-ESI-Q-TOF-MS. An overview of the observed substances is shown in Table 4. Some of the photodegradates shown in Table 4 were preferably formed on UVA irradiation, others exclusively on UVC irradiation. The latter gave rise to many more transformation products. In particular, the effect of hydroxyl group substitution was recognized when using different radiation sources. Alkaline pH and UVC irradiation yielded relatively many photodegradates with hydroxyl substituents, whereas these products could not be identified on UVA irradiation, see green marks in Figure 6. Photoinduced deconstruction of the piperazine moiety seemed independent of pH, see orange marks in Figure 6. The same observation was made for reactions of the ethyl substituent on the quinolone nitrogen, cf. purple marks in Figure 6. Although it cannot be ruled out that different mechanisms might lead to identical transformation products, it could be concluded that pH independent UVA and UVC photoinduced chemistry led to the fragmentation of the piperazine ring. The ethyl substituent reactions might stem from hydroxyl radical and hydroxide chemistry depending on pH, as hydroxide acts as radical scavenger for hydroxyl radicals. At last, fluorine substitution by and addition of hydroxyl groups might also be due to both mechanisms, depending on pH. An overview of the observed transformation products is given in Figure 6. As indicated in Table 4, seven not previously reported products were proposed based on MS 2 and MS 3 experiments.
Following kinetic analysis of the c-t diagrams, see Figure 4, most of the transformation products could be described as intermediate products. While only the intermediate with m/z = 332 due to UVA irradiation possessed a life-time well above 30 min, all photoproducts due to UVC irradiation were eliminated within 15 min, cf. Figure 4. Under suitable neutral and slightly alkaline conditions, UVC irradiation led to faster elimination of LOM and its photoproducts than UVA irradiation.

Assessment of Ecotoxicity
To assess the ecotoxicity of the resulting products and intermediates of the photoinduced elimination of LOM, IC50, and MIC values were determined. The MIC value of LOM against B. subtilis was found 0.17 μg•mL −1 , and 0.92 μg•mL −1 against P. fluorescens. The IC50 value of LOM against B. subtilis was 0.09 μg•mL −1 and 0.22 μg•mL −1 against P. fluorescens. As discussed in previous studies, IC50 values can be determined more accurately than MIC values due to their graphical representation [9]. Yet, an error of a factor of 2 is negligible due to the dilution assay format. A factor of 4 between IC50 and MIC in the case of P. fluorescens is rather large. Example curves for IC50 determination are given in Figure 7. As expected, the longer the LOM solution was irradiated with UVA light, the higher the IC50 grew and the least activity remained. The comparison between MIC and IC50 values depending on time of irradiation by UVA and UVC is shown in Table 5. In general, activity decreased with irradiation times where UVC irradiation appeared more effective than UVA, as can be seen from the higher MIC values at equal irradiation times. Since both bacteria represent Gram-positive and Gram-negative species ubiquitous in the aquatic environment, the lack of activity of a compound or compound mixture has been proposed as means to assess the ecotoxicity [11]. The advantage of the assay is its ease of use. While QSAR analyses often rely on the knowledge of the exact structures of the transformation products after AOP application, MIC or IC50 values represent sum parameters and therefore reflect the activity in total, hence answering indirectly to an assumption that transformation products might be more ecotoxic than the initial drug itself.
To address the issue whether transformation products are less or more toxic than their parent drug, QSAR analyses represent a common approach, since the synthesis of the suggested products is usually expensive, and no corresponding standards were available in this case. QSAR analysis was carried out on the basis of the chemical structures of the known and newly identified products of LOM, see Table 4. The results for selected species are presented in Table 6. Table 6. QSAR analysis of the phototransformation products of LOM with less (green) and more hazardous (red) photoproducts as compared to the parent compound. The photoproducts are indicated by their m/z values. The activities against the organisms are given as concentration. Most of the photoproducts observed in this work were predicted less toxic by the QSAR methods. The result was expected since the groups known to be responsible for the efficacy of fluoroquinolones were altered or deconstructed through irradiation or photoinduced chemistry. Differences in predictions against the same organisms were due to different programs or databases contained therein. Values for Daphnia magna were not obtained from the QSAR toolbox. A direct comparison could be achieved for fathead minnow. Here, the values differed by several orders of magnitude, and the toxicity was predicted differently for several product structures, cf. Table 6. This can be traced back to different databases and calibration models contained in the two programs. The prediction and computation of t.e.s.t. software is based on the database of EUCAST, while the QSAR toolbox calculation is based on the data of the European Chemicals Bureau. A literature search for reference data remained unsuccessful. Despite the different absolute values, both predictions were consistent with reference to the parent drug. Photoproducts were mostly predicted potentially less toxic. An exception was the product with m/z = 324, which strongly resembled the educt after elimination of the ethyl substituent. Yet, QSAR analyses do not take concentrations into account, hence do not reflect the ecotoxicological activity of a mixture. In this respect, MIC and IC50 values provide total activity information, although the extent of relevance of the model bacteria to the aquatic environment has not been proven yet.

Daphnia
When irradiation is considered for removal of pharmaceuticals before entering the environment, degradation rate constants in combination with MIC-or IC50values allow to determine the irradiation time according to Equation (4). The equation relates activity against bacteria with compound transformation or degradation. Hence, the time to remove potential ecotoxicological hazard from a sample containing LOM could be estimated. The values obtained for LOM samples in MilliQ water and effluent exposed to UVC irradiation are shown in Table 7. An irradiation of 10 min was calculated sufficient to remove efficacy against the investigated microorganisms P. fluorescens and B. subtilis regardless of pH and water matrix. In acid milieu, tact is highest. The comparison between different water matrices showed that a longer irradiation time was necessary to compensate for the presence of other compounds and light absorbing substances and to remove the risk of ecotoxicity in effluent water having pH 8.
The use of UV radiation for large-scale compound removal in WWTPs would lead to rather high energy consumption. Cost of goods for sufficiently large UVA and UVC lamps may also significantly increase treatment costs. Nonetheless, UV treatment is applied for microbiological treatment of drinking water. As UV irradiation proved a rather efficient measure for pharmaceutics elimination, UV treatment could be performed on demand. This might be achieved through effluent monitoring and switching on irradiation at need.

Conclusions
The investigation of water samples from the German river Erft showed the presence of the fluoroquinolone LOM. Photoinduced removal of LOM was investigated in model water and effluent at acidic, neutral, and basic milieu und UVA and UVC irradiation using HPLC-MS n techniques. As AOP catalysts, hydrogen peroxide and titanium dioxide were used. First order kinetic models applied to concentration-time plots of LOM and its phototransformation products showed that LOM degraded fastest at pH 8 in the absence of hydrogen peroxide and titanium dioxide. Products were eliminated within 10 min of UVX irradiation. Known and novel transformation products of LOM were identified using MS n . Both MIC/IC50 determination against P. fluorescens and B. subtilis and QSAR analyses suggested the removal of ecotoxicological hazard after sufficiently long irradiation. Matrix effects of effluent or surface water required prolonged irradiation in comparison to model water. With the help of degradation rate constants and activity parameters such as MIC and irradiation time until absence of activity, the potential ecotoxicity might be predicted using Equation (4). This investigation of LOM as an example might support the search for suitable AOPs as fourth purification steps in WWTPs and the prediction of treatment time.