Understanding the Coupling Effect between Lignin and Polybutadiene Elastomer

: From an environmental and economic viewpoint, it is a win–win strategy to use materials obtained from renewable resources for the production of high-performance elastomer composites. Lignin, being a renewable biomass, was employed as a functional ﬁller material to obtain an elastomer composite with a higher degree of mechanical performance. In the presence of a suitable coupling agent, an elevated temperature was preferred for the reactive mixing of lignin with polybutadiene rubber (BR). It is quite fascinating that the mechanical performance of this composite was comparable with carbon black-ﬁlled composites. The extraordinary reinforcing behavior of lignin in the BR matrix was understood by an available model of rubber reinforcement. In rubber composite preparation, the interfacial interaction between polybutadiene rubber and lignin in the presence of a coupling agent enabled the efﬁcient dispersion of lignin into the rubber matrix, which is responsible for the excellent mechanical properties of the rubber composites. The rubber composites thus obtained may lead to the development of a sustainable and cost-effective end product with reliable performance. This novel approach could be implemented in other type of elastomeric materials, enabling a genuine pathway toward a sustainable globe.


Introduction
In recent decades, the usage of fossil fuel became a global concern, mainly due its impact on the environment [1]. For a solution, several ways have been implemented by researchers, where the replacement of petroleum-derived products with renewable alternatives is the crucial one. Furthermore, the reusability of solid wastes obtained from several industries is also considered as a pathway toward sustainability [2]. However, it is a challenging task to reuse waste materials and convert it into a high-end product. As a result, the techniques such as incineration and landfilling have been adopted worldwide to dispose solid wastes. A pollution problem has originated from these activities that is quite threatening from an environmental aspect [3]. To address this issue, the transformation of existing waste materials into an alternative renewable material is the prime objective. 2 of 15 Lignin, a major constituent of renewable biomass and a co-product of the paper and pulp-making industry, might be a potential feedstock that can overcome all the obstacles on the way toward sustainability [4]. Generally, lignin is considered as a low-cost waste material obtained from the paper and pulp-making industry, and it is mostly thrown away or sometimes used as a burning fuel. It has been reported that a huge amount (≈75 million tons per year) of lignin is produced by paper-making industries, but a very minute quantity (≈2%) is utilized to develop value-added product [5]. However, recent study reveals that lignin was utilized in a wide range of conventional and smart applications. Lignin was introduced into the polymer matrix to improve the thermal stability [6] and flame retardancy [7] of the composites. On the other hand, lignin was utilized to develop shape memory polymer [8], self-healing elastomer [9], and renewable skin with programmable and switchable electrical conductivity [10]. Furthermore, lignin was used in energy storage [11] and 3D printing applications [12]. Lignin was also used as a precursor to produce high-performance carbon fibers [13]. It is also to be noted that a homogeneous dispersion of lignin into the polymer matrix is mandatory to get the desired properties. Indeed, the dispersion of lignin in a soft elastomar matrix is a challenging task, as lignin is a crosslinked biopolymer having a hyperbranched structure. A lot of recent studies ensured that a chemical modification of the lignin surface could solve the issue. In a recent study, a suitable coupling agent was used to improve the interaction of lignin with elastomer, which provides excellent mechanical properties [14]. Various methods were introduced in this regard, which include esterification of the available -OH groups on the lignin surface [15], the allylation of lignin moeity [16], development of phenolated lignin-based materials [17], and polymer grafting on the lignin surface [18].
In this study, we are mainly focusing on the reinforcing characteristics of lignin-based elastomer composites. In the field of elastomer technology, carbon black is considered to have a dominating role as a reinforcing filler material. However, carbon black is a nonrenewable material, as it is produced by the incomplete combustion of petroleum-derived product [19]. Therefore, the exploration of a suitable alternative of carbon black is of great significance. Considering this fact, in a few studies, lignin was used as an alternative filler material in various forms as functional additive [20], reinforcing biofiller [21], and nano-biochar [22].
Some of the research works also reported a hybrid filler system, where lignin was used along with carbon black [23], silica [24], and layered double hydroxides [25]. However, still, the application of lignin as a potential reinforcing filler is a matter of debate, as it was difficult to achieve a comparable reinforcing efficacy with respect to carbon black and other conventional fillers.
With this background, a new target has been set, where the replacement of reinforcing filler material such as carbon black by lignin is the main goal. In this study, a unique reactive mixing technique was developed, where the interaction of lignin with rubber matrix could be realized by using a chemical coupling agent. Furthermore, the impact of different lignin concentration, the quantification of rubber to lignin interaction by swelling and dynamic mechanical experiments, the alteration of the overall crosslinking densities, etc. are reported. Finally, this study inspects the results in the light of existing mathematical models regarding rubber-filler interaction, network density, and so on. It is interesting to mention in this context that this newly developed technique might open a new window in the field of tire technology in a sustainable way.

Fabrication of the Composites
A multi-step compounding strategy was adopted to develop BR-lignin composites. The recipe of all the composites is given in Table 1. A detailed description of each individual step is given below.
Step 1: In this step, BR was masticated in a laboratory scale two-roll mill (Polymix 110 L, size: 203 × 102 mm 2 , Servitech GmbH, Wustermark, Germany) for 1 min. Then, zinc oxide, stearic acid, and lignin were added one by one, and the mixing was continued for 10 min to get a homogeneous mixture. The mixing was carried out at room temperature with a friction ratio of 1:1.4.
Step 2: The obtained mix was incorporated into the chamber of an internal mixer (Haake TM Rheomix, Thermo Electron GmbH, Karlsruhe, Germany) and run for 10 min at 155 °C with a rotor speed of 50 rpm. The mixing temperature (155 °C) was chosen, which is above the glass transition temperature of lignin ( = 153 °C).
Step 3: In this step, the mix obtained from step 2 was further incorporated into the internal mixer along with TESPT and mixed at 120 °C for 10 min with a rotor speed of 50 rpm.
Step 4: Finally, the compound obtained from step 3 was mixed with CBS, DPG, and sulfur (curatives) in a two-roll mill at room temperature for 10 min with a friction ration of 1:1.4.
To study the rheometric characteristics, the mixed compounds were subjected to a moving die rheometer (Scarabaeus SIS-V50). Around 5 g of sample was placed into the machine, and the measurement was done at 160 °C for 1 h with a frequency of 1.67 Hz.
Optimum curing time ( ) was obtained from the rheometric curve (torque vs. time). Utilizing this time ( ), the mixed compounds were cured using a hot press (Fontijne Holland, Model TP400, Delft, The Netherlands) at 160 °C with 150 KN force. After curing, the cured sheet (dimension = 110 × 100 × 2 mm 3 ) was placed at ambient temperature for 24 h before performing any tests.

Fabrication of the Composites
A multi-step compounding strategy was adopted to develop BR-lignin composites. The recipe of all the composites is given in Table 1. A detailed description of each individual step is given below.
Step 1: In this step, BR was masticated in a laboratory scale two-roll mill (Polymix 110 L, size: 203 × 102 mm 2 , Servitech GmbH, Wustermark, Germany) for 1 min. Then, zinc oxide, stearic acid, and lignin were added one by one, and the mixing was continued for 10 min to get a homogeneous mixture. The mixing was carried out at room temperature with a friction ratio of 1:1.4.
Step 2: The obtained mix was incorporated into the chamber of an internal mixer (Haake TM Rheomix, Thermo Electron GmbH, Karlsruhe, Germany) and run for 10 min at 155 • C with a rotor speed of 50 rpm. The mixing temperature (155 • C) was chosen, which is above the glass transition temperature of lignin (T g = 153 • C).
Step 3: In this step, the mix obtained from step 2 was further incorporated into the internal mixer along with TESPT and mixed at 120 • C for 10 min with a rotor speed of 50 rpm.
Step 4: Finally, the compound obtained from step 3 was mixed with CBS, DPG, and sulfur (curatives) in a two-roll mill at room temperature for 10 min with a friction ration of 1:1.4.
To study the rheometric characteristics, the mixed compounds were subjected to a moving die rheometer (Scarabaeus SIS-V50). Around 5 g of sample was placed into the machine, and the measurement was done at 160 • C for 1 h with a frequency of 1.67 Hz.
Optimum curing time (t 90 ) was obtained from the rheometric curve (torque vs. time). Utilizing this time (t 90 ), the mixed compounds were cured using a hot press (Fontijne Holland, Model TP400, Delft, The Netherlands) at 160 • C with 150 KN force. After curing, the cured sheet (dimension = 110 × 100 × 2 mm 3 ) was placed at ambient temperature for 24 h before performing any tests. 100 --50 1 Each ingredient was taken in phr (parts per hundred parts of rubber). Except for the above ingredients, each composite contains 5 phr zinc oxide, 2 phr stearic acid, 1.5 phr CBS, 1.8 phr DPG, and 1.5 phr sulfur. The optimum amount of TESPT was investigated in our previous study, and it is maintained in this study accordingly. It is to be noted that the composite containing carbon black was mixed at room temperature, unlike other lignin-rubber composites.

Characterizations
The uniaxial tensile tests were carried out using dumbbell-shaped specimens (as per DIN S2). A universal testing machine (UTM, Zwick/Roell-Z010, Ulm, Germany) was used for the test according to DIN 53504. All the measurements were executed at ambient temperature, and a cross-head speed of 200 mm/min was used. Information such as tensile strength, percentage elongation at break, 50% modulus, and 100% modulus were obtained from this measurement.
Dynamic mechanical properties of the compound were studied using a dynamic mechanical analyzer (GABO Qualimeter, Eplexor 150N, Ahlden, Germany). A rectangularshaped sample with dimensions of 32 × 10 × 2 mm 3 was taken for this purpose. The measurement was carried out under tension mode with a varying temperature between −120 and 60 • C with a heating rate of 2 K/min at a frequency of 10 Hz under 1% static and 0.2% dynamic strain. A strain sweep measurement was also performed with a dynamic mechanical analyzer (GABO Qualimeter, Eplexor 2000N) to investigate the dynamic mechanical properties with varying strain at constant temperature. A specimen with similar dimensions (32 × 10 × 2 mm 3 ) was taken, and the test was carried out under 60% static strain at a constant frequency of 10 Hz. A wide range of variation of dynamic strain (0.01-30%) was given to the specimen.
Equilibrium swelling experiments were carried out to study the crosslink density of the composites. A small piece (≈0.5 g) of specimen was swelled in toluene for 72 h (ASTM D 6814-02). A fresh solvent was used within each 24 h interval. Then, the specimen was taken out from toluene, and using a filter paper, the excess solvent onto the surface was wiped off. After taking the weight of the specimen (considered as swollen weight), it was allowed to dry at room temperature for 24 h. Once it achieved a constant weight, the dry weight of the specimen was taken. Then, by using the Flory-Rehner equation [26], the crosslink density, υ, of the sample was calculated. V r is the volume fraction of rubber in swollen mass, χ is the solvent-rubber interaction parameter and the value 0.30 [27] was taken for this study, and V s is the molar volume of the toluene.
The surface morphology of the specimens was investigated utilizing a scanning electron microscope (SEM; Zeiss Ultra Plus, Carl Zeiss Microscopy GmbH, Jena, Germany).
The specimen was cut using an ultramicrotome at −180 • C, and then sputter coating with platinum was carried out before performing the experiment.
A differential scanning calorimetry (DSC) study was done for all specimens using Q100 (TA Instruments, New Castle, DE, USA) coupled with an auto-sampler in the temperature range of −100 • C to +180 • C at the scanning rate of 10 K/min in nitrogen atmosphere.

Results and Discussion
The rheometric torque profile of different BR compounds is shown in Figure 2a, and the extent of curing or ultimate rheometric torque (R ∞ = M H − M L ) was represented as a bar diagram in Figure 2b. The values of scorch time (t 2 ), optimum cure time (t 90 ), maximum torque (M H ), minimum torque (M L ), and cure rate index (CRI) were summarized in Table 2. CRI was calculated using the following equation It can be observed from Table 2 and Figure 2b that the values of minimum torque, maximum torque, and the extent of cure were found to be gradually enhanced with the increasing concentration of lignin. This was due to the enhancement of viscosity in the presence of rigid filler particles. It was found that 30 phr lignin in BR showed a lower ultimate rheometric torque value as compared to a gum compound without any filler. On the other hand, lignin-containing composites from 50 to 90 phr displayed the expected trend by increasing the value of extent of cure. Germany). The specimen was cut using an ultramicrotome at −180 °C, and then sputter coating with platinum was carried out before performing the experiment. A differential scanning calorimetry (DSC) study was done for all specimens using Q100 (TA Instruments, New Castle, DE, USA) coupled with an auto-sampler in the temperature range of -100 °C to +180 °C at the scanning rate of 10 K/min in nitrogen atmosphere.

Results and Discussion
The rheometric torque profile of different BR compounds is shown in Figure 2a, and the extent of curing or ultimate rheometric torque ( = − ) was represented as a bar diagram in Figure 2b. The values of scorch time ( ), optimum cure time ( ), maximum torque ( ) , minimum torque ( ) , and cure rate index (CRI) were summarized in Table 2. CRI was calculated using the following equation ( It can be observed from Table 2 and Figure 2b that the values of minimum torque, maximum torque, and the extent of cure were found to be gradually enhanced with the increasing concentration of lignin. This was due to the enhancement of viscosity in the presence of rigid filler particles. It was found that 30 phr lignin in BR showed a lower ultimate rheometric torque value as compared to a gum compound without any filler. On the other hand, lignin-containing composites from 50 to 90 phr displayed the expected trend by increasing the value of extent of cure. Now, considering the rate of vulcanization, it was evident that the scorch time ( ) showed a marginal increment with increasing concentration of lignin. However, in case of the composite with 90 phr loading, a sudden enhancement of was noticed. The optimum cure time ( ) showed an interesting trend. The values were increasing with increasing concentration of lignin, except for the composite containing 30 phr of lignin, which showed the highest value among the other lignin-filled composites. It is believed that sulfur vulcanization reaction is facilitated in basic medium, and here, the lignin has a pH value of 12.1. So, if this basic nature of lignin is considered, it could facilitate the sulfur vulcanization reaction rate. In reality, there is no such clear trend in the rheometric curves that can be explained by the pH factor of the filler. Since these rubber composites are multi-component systems with many chemical functional groups, a clear understanding of the vulcanization or rheometric profile is difficult at least in this stage. Nevertheless, the higher the value of CRI, the higher the rate of vulcanization will be, and therefore, 50 phr lignin-containing composite showed the highest value of CRI. Now, considering the rate of vulcanization, it was evident that the scorch time (t 2 ) showed a marginal increment with increasing concentration of lignin. However, in case of the composite with 90 phr loading, a sudden enhancement of t 2 was noticed. The optimum cure time (t 90 ) showed an interesting trend. The t 90 values were increasing with increasing concentration of lignin, except for the composite containing 30 phr of lignin, which showed the highest t 90 value among the other lignin-filled composites. It is believed that sulfur vulcanization reaction is facilitated in basic medium, and here, the lignin has a pH value of 12.1. So, if this basic nature of lignin is considered, it could facilitate the sulfur vulcanization reaction rate. In reality, there is no such clear trend in the rheometric curves that can be explained by the pH factor of the filler. Since these rubber composites are multi-component systems with many chemical functional groups, a clear understanding of the vulcanization or rheometric profile is difficult at least in this stage. Nevertheless, the higher the value of CRI, the higher the rate of vulcanization will be, and therefore, 50 phr lignin-containing composite showed the highest value of CRI.
The stress-strain curves and other parameters obtained from tensile stress-strain measurements of all the composites are presented in Figure 3. It was found that the mechanical properties of the composite with 30 phr loading were more or less similar to those of the gum compound. On the other hand, in the case of the composite with a higher amount of lignin-for example, 50 phr loading, a remarkable improvement in tensile strength and elongation at break was noticed as compared to reference gum compound. As expected for the composites with further higher loading, a marginal change in tensile strength and a substantial reduction in percentage elongation at break were observed. Although TESPT improves the dispersion of lignin into the elastomer matrix, but at a higher loading of lignin, the coupling efficiency of TESPT might have reduced. Due to the strong filler-filler interaction, the materials become rigid, which inhibits the stress transmission and subsequently decreases the elongation at break. However, the stress values at 100% elongation for both of the composites (70 phr and 90 phr) were significantly increased (Figure 3c). Subsequently, a comparative study was also done with reinforcing carbon black (CB)-filled composites. Surprisingly, it is found that the mechanical properties of the 50 phr lignin-containing composite are comparable with CB-filled composite at similar loading in terms of tensile strength, percentage elongation at break, and stress at 100% break.
To get more insight about rubber-filler interaction and filler reinforcement, the stressstrain behavior was reanalyzed by using a phenomenological equation proposed by Mooney and Rivlin [28,29], which is as follows, where σ is the nominal stress, λ is the extension ratio, and C 1 , C 2 are the Mooney-Rivlin constants.
Equation (3) can be used directly for an unfilled elastomer compound. In case of filled composites, a correction factor, namely the strain amplification factor (x) was introduced, using the following equation, x = 1 + 2.5φ + 7.6φ 2 , where φ is the volume fraction of filler. The extension ratio is modified by λ= 1 + εx, where ε is the strain. Here, pre-conditioning of the sample was done by several cyclic stress-strain operations to eliminate the Mullin's effect.
The Mooney-Rivlin plots of all the composites were obtained in Figure 3d. C 1 and C 2 values were obtained from the intercept and slope of the reduced stress ( σ λ−λ −2 ) vs. 1/λ plot, respectively. Crosslink density (ν) can be calculated from C 1 by using the expression 2C 1 = νkT where ν is the crosslinking density, k is the Boltzmann constant, and T is the temperature. However, the present system is a filled crosslinked rubber, so direct extraction of the crosslinking densities is not a suitable approach. However, for the comparison purpose, this approach was used to calculate apparent crosslinking densities. The values of C1, C2, and ν were depicted in Table 3. The crosslink densities were found to be increased with increasing concentration of lignin.
In this context, to understand the chemical interaction of the rubber chain with lignin particles, the variation of dynamic modulus with strain amplitude will be interesting to focus. This phenomenon is also known as the "Payne effect" [30][31][32]. The results obtained from strain sweep experiments are depicted in Figure 4. As expected, the gum rubber sample showed almost no strain dependency in comparison with the filled sample. In case of a filled composite, a reduction in storage modulus (E') was observed after a critical strain, which was due to the breakdown of the filler-filler network. It can be mentioned here that the magnitude or the intensity of the Payne effect is more or less similar with all filled composites irrespective of the loading amount of lignin. This might be due to the strong chemical interaction between lignin and rubber molecules in the presence of TESPT, which is very similar to the silane-silica system.  However, the present system is a filled crosslinked rubber, so direct extraction of the crosslinking densities is not a suitable approach. However, for the comparison purpose, this approach was used to calculate apparent crosslinking densities. The values of C 1 , C 2 , and ν were depicted in Table 3. The crosslink densities were found to be increased with increasing concentration of lignin.
In this context, to understand the chemical interaction of the rubber chain with lignin particles, the variation of dynamic modulus with strain amplitude will be interesting to focus. This phenomenon is also known as the "Payne effect" [30][31][32]. The results obtained from strain sweep experiments are depicted in Figure 4. As expected, the gum rubber sample showed almost no strain dependency in comparison with the filled sample. In case of a filled composite, a reduction in storage modulus (E') was observed after a critical strain, which was due to the breakdown of the filler-filler network. It can be mentioned here that the magnitude or the intensity of the Payne effect is more or less similar with all filled composites irrespective of the loading amount of lignin. This might be due to the strong chemical interaction between lignin and rubber molecules in the presence of TESPT, which is very similar to the silane-silica system. To further elucidate the rubber-lignin r interactions, extension of this study has been done on the basis of agglomeration-deagglomeration phenomenon developed by Kraus [33]. According to this concept, the filler-filler network was produced by agglomeration and de-agglomeration mechanisms under dynamic strain. The equation related to this study is as follows where E 0 and E ∞ are the initial storage modulus and storage modulus at large strain amplitudes, respectively. E (γ) is the modulus at a particular strain amplitude γ, E (γ) becomes E ∞ at larger strain; E (γ) becomes E 0 at lower strain γ 0 ; γ c is considered as critical strain, where the value of (E 0 − E ∞ ) becomes half. It is strongly dependent on the type and character of the fillers, rubbers, and the dispersion level of filler into the rubber matrix; m is a constant, which depends upon the structure of fractal agglomerate of filler materials [34].
Using the data obtained from the strain sweep experiment and Equation (4), the value of critical strain was calculated, which is shown in Figure 4b. It is observed that with an increasing concentration of lignin, the value of γ c was increasing. This indicates that at a relatively lower concentration of lignin, the filler-filler network was comparatively weak compared with the composites with a higher loading of lignin. To further elucidate the rubber-lignin r interactions, extension of this study has been done on the basis of agglomeration-deagglomeration phenomenon developed by Kraus [33]. According to this concept, the filler-filler network was produced by agglomeration and de-agglomeration mechanisms under dynamic strain. The equation related to this study is as follows where and are the initial storage modulus and storage modulus at large strain amplitudes, respectively. ( ) is the modulus at a particular strain amplitude , ' ( ) becomes ' at larger strain; ´( ) becomes ´ at lower strain ; is considered as critical strain, where the value of ( − ) becomes half. It is strongly dependent on the type and character of the fillers, rubbers, and the dispersion level of filler into the rubber matrix; m is a constant, which depends upon the structure of fractal agglomerate of filler materials [34]. Using the data obtained from the strain sweep experiment and Equation (4), the value of critical strain was calculated, which is shown in Figure 4b. It is observed that with an increasing concentration of lignin, the value of was increasing. This indicates that at a relatively lower concentration of lignin, the filler-filler network was comparatively weak compared with the composites with a higher loading of lignin. The equilibrium swelling experiment was further carried out to get more information regarding the influence of rubber-filler interaction on the network density of the composites. Figure 5a shows the variation of volume fraction of rubber in swollen gel. It was found that the value of increases with increasing lignin content. This means that the availability of free space was reduced and further solvent penetration was prevented. This observation corresponds to an improved rubber-filler interaction, which further corroborates with the previous results. The Cunneen-Russel equation [35] (Equation (5)) was utilized to assess the rubber-filler interaction.
where and are the volume fraction of the gum and filled compound respectively, z is the weight fraction of the filler, and a and b are constants. A plot of versus The equilibrium swelling experiment was further carried out to get more information regarding the influence of rubber-filler interaction on the network density of the composites. Figure 5a shows the variation of volume fraction V r f of rubber in swollen gel. It was found that the value of V r f increases with increasing lignin content. This means that the availability of free space was reduced and further solvent penetration was prevented. This observation corresponds to an improved rubber-filler interaction, which further corroborates with the previous results. The Cunneen-Russel equation [35] (Equation (5)) was utilized to assess the rubber-filler interaction.
where V r 0 and V r f are the volume fraction of the gum and filled compound respectively, z is the weight fraction of the filler, and a and b are constants. A plot of Furthermore, the extent of reinforcement was obtained by using the Lorenz-Park equation [36] Q where Q f and Q g represents the amount of solvent entrapped per unit weight of gum and filled compound, respectively. This could be calculated from the swelling data by the following expression for filled (Q f ) and gum (Q g ) compound. z is the weight fraction of filler; a and b are constants. A lower value of Accordingly, from Figure 5b, it was clearly seen that the value of was represented in Figure 5b. A straight line having a positive slope (higher value of a and lower value of b indicates the improved rubber-filler interaction. In addition, the value of less than 1 for all the composites defines the reinforcing nature of kraft lignin. Furthermore, the extent of reinforcement was obtained by using the Lorenz-Park equation [36] = + where Qf and Qg represents the amount of solvent entrapped per unit weight of gum and filled compound, respectively. This could be calculated from the swelling data by the following expression for filled (Qf) and gum (Qg) compound. z is the weight fraction of filler; a and b are constants. A lower value of indicates better rubber-filler interaction.
Accordingly, from Figure   The measurement of crosslink density (shown in Figure 5c as a bar diagram) further explains the reinforcing behavior of lignin in the presence of TESPT. An enhancement in crosslink density was noticed with increasing concentration of lignin and reached a maximum value at highest loading (90 phr) of lignin. This refers to the significant contribution of lignin as a filler material into the formation of network structure.
The swelling index, which was a direct measure of resistance to swelling, could be obtained by using the following expression.
where M sw , M dw , and M iw are the swollen weight, dried weight, and initial weight of the elastomer composites, respectively. The swelling index of all the composites was shown in Figure 5d as a bar chart. The swelling index signifies the availability of free space in the composite for the solvent to penetrate. If less space was available due to the formation of the dense network structure, the solvent could not be imbibed into the matrix, resulting in the value of the swelling index going down. It was shown in Figure 5d that the swelling index value was going to decrease with the increasing lignin content. It means that a relatively denser network structure was formed with increasing lignin concentration, and consequently, a resistance was offered toward swelling. So, these findings were in good agreement with the results obtained from rheometric analysis and tensile experiments. Dynamic mechanical analysis (temperature sweep) was performed to study the viscoelastic properties of the elastomer composite over a wide range of temperature. The storage modulus vs. temperature curve is shown in Figure 6a for all the composites with different lignin content. The storage moduli in the rubbery plateau region were gradually increased with the lignin loading, which was expected due to the hydrodynamic reinforcement. However, an unusual behavior of storage modulus at lower temperature (after glass transition temperature) can be observed. This phenomenon can be correlated with the cold crystallization behavior of BR. At the beginning of the measurement, when the sample was heated slowly from quenched state above its glass transition temperature, the crystallites were melted, which causes a significant reduction in E [37]. Further heating triggered growth of the crystal nucleus by the thermal activation of elastomer chains, and a rapid increment in E was noticed. In the case of the gum rubber without any filler as well as the composites with low amounts of lignin, the effect is very strong. The effect was minimized for 50 phr lignin-containing composites, and the same disappeared at higher loading. The process of cold crystallization might be hindered at a higher concentration of lignin due to the formation of a large number of additional crosslinks in the presence of TESPT. On the other hand, a limited number of additional crosslinks were formed at lower concentration of lignin. As a consequence, a few elastomer chains were available to take part in the cold crystallization process. In Figure 6b, the tan δ vs. temperature curve is shown for the BR/kraft lignin/TESPT composites with varying concentration of lignin in presence of TESPT. The reduction of tan δ peak height and also the shift of glass transition temperature (T g ) toward higher temperature was observed. The reduction of tan δ peak height is associated with the improved rubber filler interaction [38]. Even at higher loading, lignin was dispersed well in the presence of TESPT. A ≈6 • C shift in T g toward higher temperature was observed for the composite with high loading of lignin (90 phr) compared to the gum compound. This is an indication of good rubber-filler interaction even at higher filler loading [39]. In Figure 6b, the tan δ vs. temperature curve is shown for the BR/kraft lignin/TESPT composites with varying concentration of lignin in presence of TESPT. The reduction of tan δ peak height and also the shift of glass transition temperature (Tg) toward higher temperature was observed. The reduction of tan δ peak height is associated with the improved rubber filler interaction [38]. Even at higher loading, lignin was dispersed well in the presence of TESPT. A ≈6 °C shift in Tg toward higher temperature was observed for the composite with high loading of lignin (90 phr) compared to the gum compound. This is an indication of good rubber-filler interaction even at higher filler loading [39].
A relation between the loss factor of filled composite and gum vulcanizate can be expressed as [40,41] = 1 + 1.5 (9) where B is phenomenological interaction parameter, and the higher value of 'B' corresponds to enhanced rubber-filler interaction, and is the volume fraction of the filler. and are the values of the peaks of filled and unfilled elastomer composites.
It can be seen from Table 4 that the value of 'B' was larger in the case of 90 phr lignincontaining composites, which reveals a significant rubber-filler interaction at higher A relation between the loss factor of filled composite and gum vulcanizate can be expressed as [40,41] tan δ = tan δ m 1 + 1.5Bφ (9) where B is phenomenological interaction parameter, and the higher value of 'B' corresponds to enhanced rubber-filler interaction, and φ is the volume fraction of the filler. tanδ and tanδ m are the values of the peaks of filled and unfilled elastomer composites. It can be seen from Table 4 that the value of 'B' was larger in the case of 90 phr lignin-containing composites, which reveals a significant rubber-filler interaction at higher loading of lignin in the presence of TESPT. It was observed that the cold crystallization was more effective in case of the composites of 30 and 50 phr loading. As a result, an unexpected trend in 'B' value (not shown in Table 4) was found. Similarly, an unusual nature in tanδ vs. temperature curve for 30 phr loading was also witnessed. A further analysis was carried out by using scanning electron microscope (SEM) to assess the surface morphology of the composites. The information regarding the degree of lignin dispersion into the rubber matrix was also obtained. SEM micrographs of all the lignin-containing composites are given in Figure 7. The micrographs clearly demonstrate a good dispersion of lignin into the BR matrix in the presence of TESPT. A few agglomerates were found in case of lower concentration of lignin. However, in other cases, a uniform level of dispersion was witnessed. Particles were present at the submicron level, and no trace of agglomeration was observed. The higher degree of dispersion is another reason behind the mechanical reinforcement.
loading of lignin in the presence of TESPT. It was observed that the cold crystallization was more effective in case of the composites of 30 and 50 phr loading. As a result, an unexpected trend in 'B' value (not shown in Table 4) was found. Similarly, an unusual nature in vs. temperature curve for 30 phr loading was also witnessed. A further analysis was carried out by using scanning electron microscope (SEM) to assess the surface morphology of the composites. The information regarding the degree of lignin dispersion into the rubber matrix was also obtained. SEM micrographs of all the lignin-containing composites are given in Figure 7. The micrographs clearly demonstrate a good dispersion of lignin into the BR matrix in the presence of TESPT. A few agglomerates were found in case of lower concentration of lignin. However, in other cases, a uniform level of dispersion was witnessed. Particles were present at the submicron level, and no trace of agglomeration was observed. The higher degree of dispersion is another reason behind the mechanical reinforcement.  Table 5 summarizes the parameters obtained and calculated from differential scanning calorimetric (DSC) analysis of BR/kraft lignin/TESPT composites with different  Table 5 summarizes the parameters obtained and calculated from differential scanning calorimetric (DSC) analysis of BR/kraft lignin/TESPT composites with different concentrations of lignin. In the DSC experiment, the glass transition temperature can be detected due to changes in the heat capacity (∆Cp) of the glassy state and rubbery state. Other important information could be obtained from DSC analysis, which is the fraction of the immobilized elastomer chain (x im ) and can be quantified using the following expression: [42] x im = 1 − ∆Cp ∆Cp 0 (1 − w) where ∆Cp 0 was the change in heat capacity of gum rubber compound and w was the weight fraction of lignin. In Table 5, the effect of different concentrations of lignin on the heat capacity and fraction of an immobilized elastomer chain is shown. It was clear that with an increasing concentration of lignin, the ∆Cp value was decreasing. The decrease of the heat capacity of a rubbery phase was only possible if the polymers were immobilized, and this would be possible if lignin particles interfere with the degrees of freedom of the elastomer chain, indicating a strong chemically bound rubber on the surface of the lignin. As a result, the extent of the immobilized rubber fraction was also increasing with lignin concentration.

Conclusions
Considering certain environmental issues, an effort was given to utilize lignin as a reinforcing filler component in polybutadiene rubber. The high temperature-driven melt mixing process enables developing strongly reinforced BR composites. A variation of the lignin loading explores the optimum loading of lignin, and excellent mechanical properties were obtained with 50 phr lignin-containing composites, which is even better than the carbon black filler BR composites with similar loading. A comparable property was noticed with increasing concentration of lignin (70 and 90 phr), but the 100% modulus value showed a significant enhancement compared to the other composites. It indicates that a higher amount of lignin would have an impact on the crosslink network density. This result is in good agreement with the results obtained from equilibrium swelling experiments. Dynamical mechanical analysis provides a clear idea regarding the rubber-filler interaction. A distinct knowledge regarding the degree of dispersion was gained from scanning electron microscopic measurements. The effect of lignin particles on the crosslink network structure of the composites was also analyzed in terms of the fraction of the immobilized rubber chain, which was calculated from DSC analysis. Therefore, from this study, it is concluded that lignin could be considered as a potential reinforcing filler (obtained from renewable resources) as an alternative of existing petroleum-derived filler materials.