Optimizing N Fertilization for Increasing Yield and Profits of Rainfed Maize Grown under Sandy Loam Soil

The optimum dose of fertilizers for crops varies with soil, agro-ecology, and crop management practices. Optimizing application dose is critical to reduce nutrient loss to the environment and increase nitrogen use efficiency (NUE), crop yields, and economic return to farmers. An experiment was conducted to determine the optimum N dose for increasing maize (Zea mays L. cv, Manakamana-3) yield, NUE, and farm profits under rainfed conditions. Five levels of N (0, 60, 120, 180, and 240 kg ha−1), and a non-fertilized treatment were tested in a randomized complete block design with three replications. Effects of each treatment on yield and yield attributing traits, plant lodging and Sterility (plants with no cob or grain formation), NUE, and stay green trait of maize were recorded. Application of N above 120 kg ha−1 (N120) did not have any significant effects on yield and yield components. Nitrogen, at N120 and above, produced highly fertile plants (though sterility slightly increased at N180 and N240), higher N uptake, and lower dead leaf area (18–27%). N120 produced the highest agronomic; yield increase per unit of N application (AEN—26.89 kg grain kg−1 N) and physiological efficiency of N (PEN—42.67 kg grain kg−1 N uptake), and net benefit (USD 500.43). Considering agronomic, economic, and NUE factors, an N dose of 120 kg ha−1 was found optimum for the cultivation of rainfed maize (Manakamana-3) under sandy loam soil.


Introduction
Maize is a major cereal crop for livestock feed, fuel, fodder, and human nutrition across the world. Globally, 1148.49 million tons of maize is produced from 197.20 million hectares (ha) area with a productivity of 5.82 t ha −1 and consumed mostly as feed (61%), food (17%), and industrial raw material (22%) [1]. In Nepal, current maize production is 2.71 million tons from a 0.96 million hectare area with an average productivity of 2.84 t ha −1 [2]. Besides being a staple crop of the mid-hill region, maize is the chief ingredient of livestock feeds, especially for rapidly growing poultry [3,4]. Despite the cultivation of several new maize hybrids and improved varieties, national production fails to fulfill total demand, and the country imports maize in large quantities every year [5,6]. Maize productivity is low compared to other South Asian countries (Pakistan-5.12 t ha −1 , Bangladesh-8.02 t ha −1 , Sri Lanka-3.87 t ha −1 ) [1]. Low productivity is associated with several edaphoclimatic constraints. One of the major reasons for low productivity is the inefficient use of fertilizers, thus a large yield gap between farmer's practice and attainable yield is prevalent [7][8][9].
Among the essential plant nutrients, nitrogen (N) is the most limiting one [10][11][12]. However, both excess and deprived application could be detrimental to plants. Nitrogen shortage during the vegetative stage directly affects root development, stem elongation, (Koppen-Geiger climatic class: temperate climate with dry winter and hot summer). The daily variation in rainfall (mm), maximum-minimum temperatures ( • C), and humidity (%) during the study period are depicted in Figure 1.

Experimental Site and Setup
A field trial was established at Directorate of Agricultural Research, Dasharathpur, Surkhet (28°30′ northern latitude, 81°47′ eastern longitude, and 490 m above mean sea level) from June to October 2020 during the summer season under rainfed conditions (Koppen-Geiger climatic class: temperate climate with dry winter and hot summer). The daily variation in rainfall (mm), maximum-minimum temperatures (°C), and humidity (%) during the study period are depicted in Figure 1. Six treatments including five doses of N fertilizer (0, 60, 120, 180, and 240 kg ha −1 ) and control-CK (without any fertilizer) were deployed in a randomized complete block design with three replications (Table 1). Replications (blocks) were one meter apart, while the distance between two plots within replication was 0.5 m. The size of each plot was 15 m 2 (5 m × 3 m) comprising four lines (20 plants in each line at 25 cm distance) at an inter-row distance of 75 cm. Two seeds per hill were sown 4-5 cm below the soil surface and later maintained as a single stand by thinning at 26 days after sowing. During field preparation, deep ploughing was performed twice followed by harrowing, and levelling. For each treatment plot, the fertilizer amount was calculated and applied uniformly to the whole plot area. Phosphorous and potassium were applied in each plot, while nitrogen (N) was applied as per treatment. Urea (46% N) was top-dressed at 28 and 46 days after sowing in two equal splits in all N applied plots. In N omission plots (N0), single superphosphate (SSP) was applied to ensure no external N application. Diammonium phosphate-DAP (18% N and 46% P2O5), muriate of potash-MOP (60% K2O), single superphosphate-SSP (16% P2O5), and other nutrients mix were applied once (basal application) before sowing at final land preparation. The other nutrients mix consisted of secondary nutrients; Magnesium (2.5%), Calcium (3%), and Sulphur (3%), and micronutrients, including Zinc (8%), Iron (0.5%), Boron (1%), Copper (0.5%), Manganese (0.5%), and Molybdenum (0.03%). Six treatments including five doses of N fertilizer (0, 60, 120, 180, and 240 kg ha −1 ) and control-CK (without any fertilizer) were deployed in a randomized complete block design with three replications (Table 1). Replications (blocks) were one meter apart, while the distance between two plots within replication was 0.5 m. The size of each plot was 15 m 2 (5 m × 3 m) comprising four lines (20 plants in each line at 25 cm distance) at an inter-row distance of 75 cm. Two seeds per hill were sown 4-5 cm below the soil surface and later maintained as a single stand by thinning at 26 days after sowing. During field preparation, deep ploughing was performed twice followed by harrowing, and levelling. For each treatment plot, the fertilizer amount was calculated and applied uniformly to the whole plot area. Phosphorous and potassium were applied in each plot, while nitrogen (N) was applied as per treatment. Urea (46% N) was top-dressed at 28 and 46 days after sowing in two equal splits in all N applied plots. In N omission plots (N0), single superphosphate (SSP) was applied to ensure no external N application. Di-ammonium phosphate-DAP (18% N and 46% P 2 O 5 ), muriate of potash-MOP (60% K 2 O), single superphosphate-SSP (16% P 2 O 5 ), and other nutrients mix were applied once (basal application) before sowing at final land preparation. The other nutrients mix consisted of secondary nutrients; Magnesium (2.5%), Calcium (3%), and Sulphur (3%), and micronutrients, including Zinc (8%), Iron (0.5%), Boron (1%), Copper (0.5%), Manganese (0.5%), and Molybdenum (0.03%).

Crop Management
Manakamana-3, a popular variety of the mid-hill region, was used for the experiment, which has a yield potential of 5.5 t ha −1 and was released in 2001 [35]. The preceding crop in the experimental site was wheat (Triticum aestivum L.). Agronomic management practices such as weeding, earthing up, and pest/disease management, etc.; were performed as required following standard protocols from the Nepal Agricultural Research Council (NARC). The crop was cultivated under rainfed conditions.

Soil, Plant and Grain Analysis
Soil samples were collected from different portions of each plot (0-20 cm depth), pooled into one composite sample for each treatment, and analyzed for their physicochemical properties (pH, OM, total N, available P and K, soil texture) at the National Soil Science Research Centre, Khumaltar, Lalitpur. Soil samples were oven-dried and passed through a 2 mm sieve before analysis. The hydrometer method was used for soil texture [36], potentiometric 1:2 for soil pH [37], Walkley and Black for organic matter [38], Kjeldahl for total N [39], Olsen's for available P 2 O 5 [40], and ammonium acetate for available K 2 O analysis [41]. The soil was characterized as a textured sandy loam (sand: 65%, silt: 35.4%, and clay: 15.4%) with slightly acidic in nature (pH: 6.45), medium level of organic matter (2.02%), total N (0.10%) and available K 2 O (125.80 mgkg −1 ), and higher amount of available P 2 O 5 (96.12 mgkg −1 ). From different literatures, it is disseminated that Zinc and Boron deficiencies are widespread in almost all agro-ecology of Nepal [42][43][44]. Terai being more prone to it, for practicing rice based cropping system [42].
After harvesting, stover and grain samples were collected from each treatment plot for determining N content and total N uptake by plants. Plant samples were oven-dried at 65 • C for 72 h [39]. The dried samples (both grain and plant) were grounded and digested with sulphuric acid before plant N analysis. Nitrogen content was determined using the Kjeldahl digestion-distillation method. The N content (percentage) in grain and plant samples were later used for the determination of different components of nitrogen use efficiencies (NUEs).

Agro-Morphological Traits Recording
Plant height and ear heights (length between ground level to the base point of the uppermost ear in maize plant) were recorded based on observations of five sample plants from central two rows of the plots, whereas whole plot observation was carried out for plant lodging and sterility, and later expressed in percentage. Plants without cobs or cobs without kernels were considered sterile. Manual harvesting of the plants was performed 5 cm above the ground in each plot; 5 m length from two central rows (5 m × 1.5 m = 7.5 m 2 ) excluding border rows. Stover yield (kg plot −1 ) was recorded after cobs were removed, oven-dried, and moisture corrected based on oven-dried weight [45]. The total number of cobs recovered were counted to record the number of ears harvested in each treatment.
Grain yield (kg plot −1 ), and hundred-grain weight (g) were recorded after proper drying and shelling of the cobs. The moisture percentage in the grain was estimated with a moisture tester (Wile 55, Farm comp Oy, Finland). The biological yield was estimated by adding grain and stover yield. After about 50% tasseling of the plants, leaf senescence scoring in each plot was performed by observing the central two rows of the plot. Four readings were taken at one-week intervals. We used a scale of 0 to 10 by dividing the estimated area of the dead leaf by 10; scale 1 indicates 10% dead leaf area, 2 indicates 20% dead leaf area, etc. The standard data recording protocol of Zaman-Allah et al. [46] was followed for all the studied parameters presented in this paper. Conversion of grain and stover yield (kg plot −1 ) to yield (t ha −1 ) were performed using the following formulas [22], Grain yield t ha −1 = Plot yield (Kg) Net harvested area (m 2 ) × (100 − recorded moisture) (100 − adjusted moisture) × 10,000 1000 (1) Stover yield t ha −1 = Plot yield (Kg) Net harvested area (m 2 ) × 10,000 1000 (2) Stover yield (kg plot −1 ) was recorded after oven drying of samples and correcting the moisture based on oven-dried weight [45]. Grain moisture was adjusted to 12.5%, and the net harvested area was in m 2 . In both the equations, multiplication factor 10,000 was used for converting area (m 2 ) into hectare and 1000 −1 for converting yield (kg) into a metric ton.

Partial Economic Analysis
Partial budgeting of N and control treatments were performed considering cultural and fertilizer related costs for the cultivation of maize in a hectare of land referring to Badu-Apraku et al. [48]. Maize threshing cost using hand sheller was used as suggested by Amare et al. [49]. Grain and stover yields were adjusted by reducing 10% from actual experimental yields to synchronize farmer's crop management practices [48]. We fixed input and labor cost, grain, and crop residues price by local market survey (Dasharathpur, and Birendranagar, Surkhet). The B:C ratio was calculated using the following formula [22]. B : C ratio = Total revenue Total cos t of cultivation (12)

Data Analysis
Data analysis was performed using Microsoft excel 2016, and ADEL-R (Analysis and Design of Experiments with R for windows) software [50]. Linear model (Equation (13)) was used to generate a one-way analysis of variance (ANOVA) to test the statistical significance of employed treatments on dependent variables (agronomic and yield attributing traits, plant lodging and Sterility, grain yield, and nitrogen use efficiency). (13) where, Y ij is the ith observation in the jth block, µ is the grand mean, T i is the effect of the treatment i (i = 1, 2 . . . 6) such that the average of each treatment level is T i = µ + T i , β j is the effect of the block j (j = 1, 2, 3) such that average of each block is B j = µ + β j , and E ij the residuals; deviation of each observation from their expected values. Significant differences between treatment means of yield attributing traits, plant lodging, sterility, grain yield, N uptake and NUEs were evaluated through post hoc Fisher least significant difference test (LSD, p ≤ 0.05).

Agronomic and Yield Attributing Traits
Increasing nitrogen doses influenced the majority of agronomic and yield-attributing traits except for the number of ears harvested at maturity (EHARV). N120 produced the tallest plant (PHT) and ear height (EHT) (224 cm and 116 cm, respectively). N dose above 120 kg ha −1 had no significant effect on plant and ear height ( Table 2). The total biomass (BY) was maximum (13.9 t ha −1 ) at 180 kg ha −1 , however, found statistically on par with the 120 kg ha −1 N dose. Dry matter partitioning in the grain increased with respective increases in N doses from N0-N240 (Table 2). Similarly, significant differences were observed for the harvest index and recorded the highest (0.46) at 60 kg N ha −1 , but at N60-N240, the effect was non-significant. The majority of agronomic and yield-attributing traits were not improved above 120 kg ha −1 and exceptionally at very lower rates in some traits; HGW, BY, and STOY (Table 2).

Leaf Senescence
Leaf senescence gradually increased with growth stages from tasseling to maturity in all treatment plots. However, N stressed plots (lower N applied plots) recorded higher scores comparatively even at the time of first scoring (66 DAS) and progressed in a similar fashion following the last scoring time (87 DAS). It was evident that dead leaf tissues were more prominent in low N plots than in higher N plots (Figure 3). At the final scoring (87 DAS), the proportion of dead leaf area in N stressed plots was 47-52%. In contrast,

Leaf Senescence
Leaf senescence gradually increased with growth stages from tasseling to maturity in all treatment plots. However, N stressed plots (lower N applied plots) recorded higher scores comparatively even at the time of first scoring (66 DAS) and progressed in a similar fashion following the last scoring time (87 DAS). It was evident that dead leaf tissues were more prominent in low N plots than in higher N plots (Figure 3). At the final scoring (87 DAS), the proportion of dead leaf area in N stressed plots was 47-52%. In contrast, N fertilized plots (at and above N120) recorded comparatively lower dead leaf area (18-27%) (Figure 3).

Grain Yield
The yield response was positively correlated with applied N doses from N0 to N240. However, after N120 yield increment rate was not significant (Figure 4). The highest gain (1.74 tons) in grain yield was achieved with N60 (N0-N60), and later 1.48 tons while increasing N dose to N120 (N60-N120). Yield advantages of 1.49, 1.44, and 1.39 t ha −1 were observed in N240, N180, and N120 treatments over N0, respectively ( Figure 5).

Grain Yield
The yield response was positively correlated with applied N doses from N0 to N240. However, after N120 yield increment rate was not significant (Figure 4). The highest gain (1.74 tons) in grain yield was achieved with N60 (N0-N60), and later 1.48 tons while increasing N dose to N120 (N60-N120). Yield advantages of 1.49, 1.44, and 1.39 t ha −1 were observed in N240, N180, and N120 treatments over N0, respectively ( Figure 5).

Grain Yield
The yield response was positively correlated with applied N doses from N0 to N240. However, after N120 yield increment rate was not significant (Figure 4). The highest gain (1.74 tons) in grain yield was achieved with N60 (N0-N60), and later 1.48 tons while increasing N dose to N120 (N60-N120). Yield advantages of 1.49, 1.44, and 1.39 t ha −1 were observed in N240, N180, and N120 treatments over N0, respectively ( Figure 5).

Nitrogen Uptake and Use Efficiency
Statistically, N uptake above N180 was not significantly different ( Figure 6). From the pattern of N uptake in different treatments, it was evident that above N180, applied fertilizer was more lost to the environment than its actual utilization by maize despite the fact that N application synchronized to crop needs (through di-ammonium phosphate-DAP at final land preparation, 50% at V6 and remaining 50% at V12 leaf stage through urea). It can be inferred from the result that the application of N above N180 is physiologically less desirable.
As expected, nitrogen use efficiency (NUE) decreased with increased N levels (Figures 6 and 7). The highest agronomic efficiency of N (AEN) was recorded at N60 (29.05 kg grain kg −1 N), which was on par with N120 (26.89 kg grain kg −1 N). The highest physiological efficiency of N (PEN) was observed at N120 (42.67 kg grain kg −1 N uptake) which was followed by N60 with 39.86 kg grain kg −1 N uptake. For total N uptake and the majority of NUE components, N180 and N240 produced similar AEN, PFPN, PEN, and IEN. Likewise, N60 and N120 had similar effects on AEN, IEN, and UEN (Figures 6 and  7). Overall, nitrogen application at 120 kg ha −1 was found to be optimum and increasing the N dose beyond that had no significant effect on any of the response variables.

Nitrogen Uptake and Use Efficiency
Statistically, N uptake above N180 was not significantly different ( Figure 6). From the pattern of N uptake in different treatments, it was evident that above N180, applied fertilizer was more lost to the environment than its actual utilization by maize despite the fact that N application synchronized to crop needs (through di-ammonium phosphate-DAP at final land preparation, 50% at V6 and remaining 50% at V12 leaf stage through urea). It can be inferred from the result that the application of N above N180 is physiologically less desirable.
As expected, nitrogen use efficiency (NUE) decreased with increased N levels (Figures 6 and 7). The highest agronomic efficiency of N (AEN) was recorded at N60 (29.05 kg grain kg −1 N), which was on par with N120 (26.89 kg grain kg −1 N). The highest physiological efficiency of N (PEN) was observed at N120 (42.67 kg grain kg −1 N uptake) which was followed by N60 with 39.86 kg grain kg −1 N uptake. For total N uptake and the majority of NUE components, N180 and N240 produced similar AEN, PFPN, PEN, and IEN. Likewise, N60 and N120 had similar effects on AEN, IEN, and UEN (Figures 6 and 7). Overall, nitrogen application at 120 kg ha −1 was found to be optimum and increasing the N dose beyond that had no significant effect on any of the response variables.

Partial Economic Analysis
Based on partial budgeting, the higher net benefit (USD 500.43) and B:C ratio was observed at N120 (Table 3). 180 kg ha −1 N (N180) is the second-best alternative producing a net benefit of USD 494.97 and a 1.51 benefit-cost ratio. Table 3 depicts the detail of total variable costs, net benefits, and benefit-cost ratios recorded in different N treatments.

Partial Economic Analysis
Based on partial budgeting, the higher net benefit (USD 500.43) and B:C ratio was observed at N120 (Table 3). 180 kg ha −1 N (N180) is the second-best alternative producing a net benefit of USD 494.97 and a 1.51 benefit-cost ratio. Table 3 depicts the detail of total variable costs, net benefits, and benefit-cost ratios recorded in different N treatments.

Effect of Nitrogen in Agronomic and Yield Attributing Traits
Nitrogen doses did not have any effects on the number of ears plant −1 as this is genetically controlled rather than by management factors. In line with our findings, Sharma et al. [51] and Ngosong et al. [52] also reported non-significant effects of N doses (0-240 kg ha −1 ) on the number of ears plant −1 . In our study, N doses above 120 kg ha −1 did not show any effects on most agronomic traits. An increment in stover yield, biomass, plant, and ear height while increasing the N dose from N0-N120 might have been attributed to increased photosynthesis, stem elongation, and overall vegetative growth [13,29]. An increase in hundred-grain weight (HGW) in response to the corresponding increase in N dose was because of continuous dry matter deposition into the grain for a longer duration.
Lower Leaf senescence with increasing N doses could be due to prolonged green color photosynthetic tissues (Figure 3). Shi et al. [53] reported that plant and ear height were similar when an N dose of 120 kg ha −1 or above was applied. Ding et al. [54] found that dry matter production in N deficient plants was significantly lower than that of N supplied plants, particularly after flowering. Cheetham et al. [55] reported maximum dry matter deposition in grain (as 200 grain-weight) when N was applied at 125 kg ha −1 .
Adhikari et al. [56] reported improved yield attributing traits of maize in response to an increase in N dose and found some improved varieties of maize (Manakamana-4, Rampur composite) performing well at 180:90:60 kg ha −1 NPK doses. Likewise, the highest biomass yield of maize at 115 kg ha −1 N was also reported from the evaluation of 0-115 kg ha −1 N doses [57]. In addition, researchers reported reduced plant height, leaf area, chlorophyll content, stay green, total dry matter, and thousand-grain weight under N deprived conditions while evaluating open-pollinated and hybrid maize varieties at 0-180 kg ha −1 N [58]. These studies sufficiently corroborate the findings of our experiment.

Plant Lodging and Sterility
Nitrogen application increases plant height and biomass production, but plants become susceptible when N applied at higher doses [19]. In addition to a higher dose of N, late-season rainfall (105 days after sowing) in combination with stormy winds might have accelerated heavy lodging in our experiment (Figure 1). A reduced supply of N results in reduced pollen shedding, poor fertilization rate, and grain filling thereby increasing more Sterility in plants [13][14][15][16]. Andrade et al. [15] pointed out that the physiological condition of maize close to the silking stage is critical to determine fertility in the cob at the final stage. Thus, in our experiment, the reason for the high Sterility recorded in N stressed plots was due to a poor supply of N at vegetative to later (silking and further) stages. The low supply of N was evident as higher leaf senescence scores were recorded from such plots (Figure 3). A decrease in plant lodging from an increasing N dose might be due to an increase in the activity of the key enzymes regulating the lignin biosynthesis, lignin content, and stalk diameter [53,59]. Moreover, the thickness of the rind number and thickness of the vascular bundle is negatively correlated to stalk lodging [53]. As in our study, significant low stalk lodging was reported in maize at 120 kg ha −1 N and a similar plant density [53]. Additionally, they reported, N application increased stem characteristics such as rind thickness, number, and thickness of vascular bundle and found a similar effect at 120 kg ha −1 N and 180 kg ha −1 N.

Leaf Senescence
Leaf senescence has a close relationship with the amount of N supply to plants throughout the crop cycle [46]. N uptake is maximum at mid-vegetative growth. Following anthesis, poor N supply from soil results in accelerated N remobilization to grains as the development of grains requires more N than maintaining vegetative tissues. Continued deficiency results in early senescence of older leaves as N is mobile in the plant system [16][17][18]60,61]. The longer the leaves stay green, chlorophyll in the leaf is maintained for a longer duration due to less N remobilization from vegetative tissues, thus increases more dry matter partitioning and can increase grain yield by 1012% [62,63]. Our findings corroborate with the results of Ding et al. [54], who reported accelerated leaf senescence in maize after anthesis in N deficient plots due to decreased chlorophyll and soluble protein contents. Similarly, reports on N deprivation at early vegetative growth resulting in a large percentage of senescent leaf area was also disseminated [64]. From the multi-season experiment, the lowest percentage of leaf senescence was reported at 120 kg ha −1 N dose while evaluating several varieties at 0 to 120 kg ha −1 N doses [65]. Likewise, Paponov and Engels [61] also reported a reduction in chlorophyll content and green leaf area due to low N supply in maize.

Grain Yield
Grain yield is a consequence of the overall genetic potential of the variety and its growing environment. We observed that N deficiency (in lower doses CK, N0, and N60) resulted in overall inferior agronomic and yield-attributing traits, a higher percentage of dead leaf tissues, lodging due to poor stalk strength, higher plant Sterility, and poor cob characteristics (Table 2, Figures 2, 3 and 8). Due to these reasons, N stressed treatments might have underperformed in comparison to their genetic potential. N stressed plants show stunted growth, yellowing of leaves, decreased green leaf tissues and photosynthesis rate, less biomass and dry matter deposition in grain, and higher sterility [13][14][15][16]25,64]. Several researchers reported increased grain yield in response to an increased application of N [51,56,[65][66][67] up to 180 kg ha −1 N, a further increase in the N level resulted in a decreased grain yield [51,55,68]. From different studies at the national and international level, a grain yield of 3.91-6.06 t ha −1 was reported at 120 kg ha −1 N application in maize during different seasons [29,56,65,68]. Thus, application of N fertilizer at 120 kg ha −1 was found optimum for the Manakamana-3 variety of maize compared with maize yield potentials reported by different studies.

Nitrogen Uptake and Use Efficiency
Our study suggests that N60 is not sufficient to supply the overall N need of the crop. Total uptake of N (48.60% more) at this dose indicates additional N uptake from Particularly in N omission plot (N0), there was little effect of P 2 O 5 and K 2 O on increasing grain yield (yielded similar to control-CK). Higher soil P 2 O 5 content (96.12 mg kg −1 ) in combination with external application might have affected in soil N availability as demonstrated by Liu et al. [69]. From long term studies, it was also disseminated that, soil application of Olsen-P above critical level (10.9-21.4 mg Kg −1 ), is less effective on increasing crop yield [70]. Potassium content in the experimental soil was medium (125.80 mg kg −1 ) and within the critical range-109-340 mg kg −1 [71], however it effects well to grain yield when integrated with nitrogenous fertilizers [72]. In Nepal, Zinc deficiency is widespread in agricultural soils, and more prominent in terai region where rice based cropping system is dominant [42]. In our study, we could not quantify the contribution of micronutrients in the grain yield of maize. However, previous study in the same location disseminated that Zinc application in maize measured up almost 3% in grain yield when applied at 20 kg ha −1 dose [22]. In addition, several studies reported, application of micronutrients in combination to macronutrients, increased yield and grain quality in maize [73][74][75]. Thus, studies on effect of Zinc and other micronutrients in maize, should be extensively done in Nepal to quantify the actual impact.

Nitrogen Uptake and Use Efficiency
Our study suggests that N60 is not sufficient to supply the overall N need of the crop. Total uptake of N (48.60% more) at this dose indicates additional N uptake from soil indigenous supply in spite of external N application. Synchronizing crop needs, N supply from the soil system is necessary to fulfill crop needs but not above the genetic and physiological potential. Any supply above the physiological needs only increases the total cost of production, not the grain yield. In our experiment, N uptake was exponential up to N180, and above that, N uptake increased at a decreasing rate. The reduction in N uptake at higher N doses might have been attributed to increments in N loss to the environment. Additionally, this might possibly be due to the less physiological needs of the crop. An increase in N uptake, as in our findings, in response to increased N doses, were reported by several studies in maize [58,[76][77][78].
Nitrogen uptake by maize from the soil, and later its assimilation and remobilization into the grain, determines the overall efficiency of applied fertilizer. Hence, uptake efficiency and utilization efficiency are two important components and increasing uptake and utilization efficiency increases the overall NUE in crops [10,25,26]. Higher values of all NUE components in lower doses of N in our study was the result of higher utilization of N uptake in developing grains and lower N loss to the environment due to the synchronization of N application time and crop needs. According to several reports, synchronizing N supply with crop demand throughout the crop period is a great strategy of reducing N loss and increasing NUE [16,79,80]. A higher dose of N application results in low NUE due to elevated N loss from ammonia volatilization, denitrification, surface runoff, and leaching [10,81]. Beatty and Wong [26] also disseminated that reducing nutrient loss could simultaneously increase nutrient efficiency through the application of optimum dose; the minimum dose that produces maximum biomass and grain yield. In accord with our findings, several previous studies reported higher NUEs at lower N levels while evaluating improved and hybrids varieties of maize at 0-280 kg ha −1 N doses [52,58,[76][77][78]80,[82][83][84]. The range of yield increment kg −1 N application was 22.01-38.16 kg at N doses of 45-150 kg ha −1 [76,80,82,84]. Results from our study are in close agreement with these studies.

Economic Return
Economic return from cropping is largely dependent on the efficiency of applied inputs including fertilizers. Inefficient application of these inputs directly increases the cost of production, leading to low economic gain from farming. Among the key factors, manure and fertilizer boast the highest share [85,86], resulting in great economic loss in the case of inefficient applications. Nepalese households rarely practice balanced fertilization to synchronize crop needs. Thus, incurring an extra 63% maize production cost, on average, compared to the firms with good agricultural practices having the same output and production technology [85]. Thus, we also performed an economic analysis in our experiment. Among the different doses, 120 kg ha −1 N was found efficient with the highest net income (USD 500.43) in comparison to other N doses. The efficiency might have been attributed to more utilization and less loss of nutrients to the soil and the environment. Few studies, from similar soil and weather conditions, have reported a net benefit of USD 881.2 from the application of recommended N dose (120 kg ha −1 ) in hybrid maize. The study also disseminated that deep placement (4-6 cm away from the plant and 5-7 cm below the soil surface) of urea in briquette form (a compressed form of prilled urea with larger granule size) could increase productivity and the economic return of maize [22]. Our preliminary study found that increasing the N dose above the recommended dose (120 kg ha −1 ) did not increase productivity and profitability, but using innovative fertilizers and application technologies might be the next alternatives. Innovative fertilizers and application technologies are emerging concepts for reducing nutrient loss to the environment and increasing NUE. The Nepal seed and fertilizer project introduced polymer-coated urea, blended fertilizers, and briquette urea in Nepal and tested their effectiveness in increasing fertilizer efficiency. The researchers reported a 25-40% reduction in N input by using polymer-coated urea and deep placement of briquette urea in maize. In addition, they reported an increase in agronomic efficiency of N from 17 kg grain per kg N to 24-28 kg of grain per kg N by using these innovative fertilizers and application technologies in maize [22,87].

Conclusions
Our preliminary results suggest that there would be no further improvement in the majority of agronomic and yield-attributing traits by increasing the N dose from the existing recommended dose (i.e., 120 kg N ha −1 ). Similarly, fertilizer use and economic efficiencies were also highest at the recommended dose. The results indicate that the current fertilizer dose is still effective and maize productivity could be increased with balanced fertilization synchronizing crop needs, which Nepalese farms are lacking [88][89][90]. However, further studies are needed to conduct across different agro-ecological zones, cropping systems and management practice to confirm this result.

Data Availability Statement:
The data presented in this study are available on request from the corresponding author.