Effect of Broccoli Residue and Wheat Straw Addition on Nitrous Oxide Emissions in Silt Loam Soil

: Nitrous oxide (N 2 O) is one of the main greenhouse gases and its emissions from vegetable production systems have brought a sustainability challenge. The objective of this study was to evaluate the potential of reducing N 2 O emissions from silt loam soil by mixing nitrogen (N)-rich broccoli ( Brassica oleracea var. italica ) residue with wheat straw or water-washed wheat straw. An experiment was conducted in randomized complete block design with ﬁve treatments; unamended or control (BS), wheat straw (+S), broccoli residue (+CR), broccoli residue and wheat straw (+CR+S) and broccoli residue and washed wheat straw (+CR+Sw) and was replicated four times. The +CR and +S were added at the rate of 3.5 kg and 2.0 kg fresh matter m − 2 and their mixtures, +CR+S and +CR+Sw, were incorporated in 3.5 kg of silt loam soil at 60% water-ﬁlled pore space (WFPS) and packed in soil microcosms. Nitrous oxide emissions were measured once a day during the 14-day of study period. Daily ﬂuxes of N 2 O were found to be reduced on +CR+W and +CR+Sw when compared to single-amended +CR treatment. Similarly, N 2 O ﬂuxes on +CR+Sw (2772 µ g N m − 2 h − 1 ) were signiﬁcantly lower than +CR+S (3606 µ g N m − 2 h − 1 ) soon after the amendment but did not vary signiﬁcantly thereafter. Moreover, the amendment mixture, +CR+S and +Cr+Sw, resulted in lower net N 2 O emissions by 73.3% and 74.2%, respectively, relative to +CR treatment. While the results clearly suggest that the +CR+S or +CR+Sw reduced N 2 O emissions, it necessitated further studies, possibly by increasing the frequency of sampling to clarify if washed wheat straw would further mitigate N 2 O emissions from the vegetable


Introduction
Nitrous oxide is the third most important contributor of the current radiative forcing (after CO 2 and CH 4 ) [1]. Apart from being a potent greenhouse gas, it is also one of the most important ozone-depleting substances in the stratosphere [2]. Out of an annual estimated figure of 6.3 Tg N 2 O-N emissions from agricultural land use [3], direct emissions from agricultural soils contribute a one-third share, i.e., 2.1 Tg N 2 O-N y −1 , and crop residuespecific emissions accounted for 0.4 Tg N 2 O-N y −1 [4], but it has so far been neglected from the management perspective [5].
All vegetable crops, particularly cole crops, are harvested before they attain physiological maturity; they need a considerable amount of nitrogenous fertilizer even at the time of harvest [6]. The recommended minimum soil mineral nitrogen (SMN) in vegetable production at harvest is 40 kg N ha −1 above the expected N uptake by vegetables [6], clearly promoting SMN content after harvest [7]. An additional source of SMN in the vegetable cropping system is the easily mineralizable N content in its residue biomass, owing to its typically low C/N ratio [8]. Enhanced SMN has climate relevance as it triggers soil microbial processes of nitrification and denitrification, leading to N 2 O emissions as their intermediate product [9]. While nitrification is most likely at ≤60% WFPS [10], the probability of denitrification will be more at higher amounts of easily available organic C inputs [11], as the denitrifiers consume O 2 with subsequent CO 2 release in the process of C substrate utilization [12]. Whatever the process and the reason, sustainable management techniques in vegetable cropping systems are needed to reduce gaseous N loss into the atmosphere and to maintain a closed N cycle within the system.
Michell et al. [13] reported a reduction in N leaching and denitrification by delayed incorporation (at the end of winter) of autumn harvested crop residue. Conversely, de Ruijter et al. [14] conducted a similar study using leek residue, but still found substantial N loss. Additionally, loss of N from undisturbed residues through other modes, for example via ammonia volatilization, could be high [15]. Introduction of catch crops after the harvest of the main crop is another frequently used option to reduce N loss but may not work for vegetable crops that are harvested in late autumn (for example, cole crops). Maximum N uptake from catch crops with subsequent N loss reduction in winter occurs only when they are sown in summer [16], and the efficiency of catch crops drastically reduces when sown later than September [17].
Similarly, few researchers [5,18,19] have documented lower N mineralization rates and consequently N 2 O emissions by amending crop residues with high C/N ratios. However, C/N ratio alone is not an accurate indicator that determines immobilization and/or mineralization potential of the residue as it does not state how microbes utilize different C and N forms [20]. Moreover, Seiz et al. [21] noticed a rapid increment of N 2 O fluxes soon after the wheat straw amendment despite a high C/N ratio, which might be attributed to the presence of readily available C fractions of straw and their utilization by the denitrifers [22]. Furthermore, an earlier study by Reinertsen et al. [23] reported that intermediately available C is also metabolized simultaneously with readily available C-compounds of straw, and cold-water washing of straw only removes readily available plant fractions.
The objective of the current study is to compare N 2 O emissions from broccoli single applications to those from the mixture of broccoli residue and wheat straw or (hot water) washed wheat straw. We assumed that washing straw with hot water would leach out both liable and intermediately liable C fractions of straw. We hypothesized that the combined residue amendment would reduce N 2 O emissions when compared to broccoli residue-only application and the amendment mixture of broccoli residue and hot-water washed straw would further intensify N immobilization.

Nitrous Oxide Emissions
Nitrous oxide fluxes varied widely among the treatments and they changed over time in the 14-day study period (Figure 1). While treatments +CR, +CR+S and +CR+Sw showed higher emissions for the first few sampling days after crop residue amendment, N 2 O fluxes on soil microcosms with BS and +S treatments were close to zero throughout the experimental period. Nitrous oxide fluxes on +CR treatment reached a peak (12,479 µg N m −2 h −1 ) on the first day and plummeted after the second day of the amendment, but surprisingly a little peak reappeared after the 11th day. Nitrous oxide fluxes were lower in +CR+S and +CR+Sw than in the +CR treatment. Peak N 2 O fluxes in +CR+S (3606 µg N m −2 h −1 ) and +CR+Sw (2772 µg N m −2 h −1 ) occurred soon after amendment and then gradually decreased over time. Treatment +CR+S maintained higher fluxes than +CR+Sw until the second day of the amendment, but there was no coherent increment of fluxes afterward. Nitrous oxide fluxes significantly reduced under +CR+S and +CR+Sw treatments when compared to the broccoli single amendment until the fifth day of soil incubation (Table 1), which followed a sudden drop of N2O fluxes in +CR treatment. There were no significant differences among the treatments in most of the sampling days afterward. Lower N2O flux rate on +CR+Sw was evident until the third day when compared to +CR+S with significantly lower fluxes on the first sampling day. Daily N2O fluxes were statistically lower in BS and +S than in other treatments. Cumulative N2O emissions during the incubation period (14 days) were significantly higher (7.7 kg N ha −1 ) in the broccoli residue-amended soil ( Figure 2). Mixing broccoli residue with straw (+CR+S) or hot water-washed straw (+CR+Sw) significantly lowered emissions when compared to +CR, however there was no significant difference among them. Moreover, the reduction potential of wheat straw and washed wheat straw (relative to broccoli single amendment) was 73.3% and 74.2%, respectively ( Table 2). Nitrous oxide fluxes significantly reduced under +CR+S and +CR+Sw treatments when compared to the broccoli single amendment until the fifth day of soil incubation (Table 1), which followed a sudden drop of N 2 O fluxes in +CR treatment. There were no significant differences among the treatments in most of the sampling days afterward. Lower N 2 O flux rate on +CR+Sw was evident until the third day when compared to +CR+S with significantly lower fluxes on the first sampling day. Daily N 2 O fluxes were statistically lower in BS and +S than in other treatments. Cumulative N 2 O emissions during the incubation period (14 days) were significantly higher (7.7 kg N ha −1 ) in the broccoli residue-amended soil ( Figure 2). Mixing broccoli residue with straw (+CR+S) or hot water-washed straw (+CR+Sw) significantly lowered emissions when compared to +CR, however there was no significant difference among them. Moreover, the reduction potential of wheat straw and washed wheat straw (relative to broccoli single amendment) was 73.3% and 74.2%, respectively ( Table 2).

Carbon Dioxide Emissions
The magnitude of average CO2 flux over the entire experimental period was in the order of +CR+S > +CR+Sw > +CR > +S > BS ( Figure 3). While the peak CO2 fluxes on +CR+S (5114 mg CO2-C m −2 h −1 ), +CR+Sw (3247 mg CO2-C m −2 h −1 ) and +CR (3597 mg CO2-C m −2 h −1 ) occurred on the third day, CO2 fluxes on BS (117 mg CO2-C m −2 h −1 ) and +S (895 mg CO2-C m −2 h −1 ) attained a slight noticeable peak only on the eighth day of amendment. In contrast to +CR+S and +CR+Sw where the elevated CO2 fluxes noticeably decreased over time with distinct small intermediate peaks in between, CO2 peaks on +CR plummeted sharply after the fifth day of amendment with no intermediate peaks after.

Carbon Dioxide Emissions
The magnitude of average CO 2 flux over the entire experimental period was in the order of +CR+S > +CR+Sw > +CR > +S > BS (Figure 3). While the peak CO 2 fluxes on +CR+S (5114 mg CO 2 -C m −2 h −1 ), +CR+Sw (3247 mg CO 2 -C m −2 h −1 ) and +CR (3597 mg CO 2 -C m −2 h −1 ) occurred on the third day, CO 2 fluxes on BS (117 mg CO 2 -C m −2 h −1 ) and +S (895 mg CO 2 -C m −2 h −1 ) attained a slight noticeable peak only on the eighth day of amendment. In contrast to +CR+S and +CR+Sw where the elevated CO 2 fluxes noticeably decreased over time with distinct small intermediate peaks in between, CO 2 peaks on +CR plummeted sharply after the fifth day of amendment with no intermediate peaks after.

Effect of Broccoli Residue on N2O Emissions
A high but ephemeral flux peak of nitrous oxide that occurred soon after the broccoli residue single amendment was obvious and consistent with the N2O emissions pattern of previous similar incubation studies [25,26]. The cumulative N2O emissions of 7.7 kg N ha −1 from broccoli residue corresponds to 3.0% of added (broccoli) residue N, which is in line with the emission factor (EF) value (>1.5%) for N rich vegetable crop residues suggested by Veltholf et al. [27] but is higher than the default value (1.25% of residue N) set by the Intergovernmental Panel of Climate Change (IPPC). Thus, our results on EF gives a guideline for IPPC methodology to devise more residue-specific emission factors. Surprisingly little N2O peaks appeared in the later stage of soil incubation and may be the result of slow but successive increase in moisture content inside soil microcosms due to high water holding capacity of silt loam soil that impedes complete percolation, leading to high denitrification rates. According to Groffman and Tiedje [28], the presence of a large proportion of micropores in fine textured soil may cause greater water retention, thus despite continuously running percolation systems, WFPS might have reached between 60% to above 70%, favoring denitrification [29,30] until the final stage of the incubation study.

Effect of Straw Addition to Soil
Wheat straw, as a sole application (+S) or in combination with broccoli residue (+CR+S or +CR+Sw), decreased daily N2O fluxes as well as net flux when compared to +CR treatment. Calculated immobilization potential of wheat straw (73.3%) was higher than what Chevas et al. [31] (60%) obtained, however that was a short-term incubation study (50 h) which might have failed to realize full immobilization potential of the straw. Nevertheless, the immobilization potential of the immobilizers has been found to be higher during laboratory incubation because of the incorporation of finely chopped and thoroughly mixed residue particles (crop residue and immobilizer) that allow better contact with immobilizing microorganisms which leads to enhanced immobilization potential of the immobilizer rather than the real field condition [32]. The decrease in N2O emissions might be attributed to a high C/N ratio of wheat straw, due to soil microbes assimilating/immobilizing mineralizable N into their biomass and making it temporarily available [33]. According to Parton et al. [33], net immobilization takes place when decomposing material has a low N content, as decomposers seek more N from other substrates, such as broccoli residue N in this case. Thus, in +CR+S and +CR+Sw, the N content of broccoli residue might have been utilized by microbes to decompose straw which otherwise could

Effect of Broccoli Residue on N 2 O Emissions
A high but ephemeral flux peak of nitrous oxide that occurred soon after the broccoli residue single amendment was obvious and consistent with the N 2 O emissions pattern of previous similar incubation studies [24,25]. The cumulative N 2 O emissions of 7.7 kg N ha −1 from broccoli residue corresponds to 3.0% of added (broccoli) residue N, which is in line with the emission factor (EF) value (>1.5%) for N rich vegetable crop residues suggested by Veltholf et al. [26] but is higher than the default value (1.25% of residue N) set by the Intergovernmental Panel of Climate Change (IPPC). Thus, our results on EF gives a guideline for IPPC methodology to devise more residue-specific emission factors. Surprisingly little N 2 O peaks appeared in the later stage of soil incubation and may be the result of slow but successive increase in moisture content inside soil microcosms due to high water holding capacity of silt loam soil that impedes complete percolation, leading to high denitrification rates. According to Groffman and Tiedje [27], the presence of a large proportion of micropores in fine textured soil may cause greater water retention, thus despite continuously running percolation systems, WFPS might have reached between 60% to above 70%, favoring denitrification [28,29] until the final stage of the incubation study.

Effect of Straw Addition to Soil
Wheat straw, as a sole application (+S) or in combination with broccoli residue (+CR+S or +CR+Sw), decreased daily N 2 O fluxes as well as net flux when compared to +CR treatment. Calculated immobilization potential of wheat straw (73.3%) was higher than what Chevas et al. [30] (60%) obtained, however that was a short-term incubation study (50 h) which might have failed to realize full immobilization potential of the straw. Nevertheless, the immobilization potential of the immobilizers has been found to be higher during laboratory incubation because of the incorporation of finely chopped and thoroughly mixed residue particles (crop residue and immobilizer) that allow better contact with immobilizing microorganisms which leads to enhanced immobilization potential of the immobilizer rather than the real field condition [31]. The decrease in N 2 O emissions might be attributed to a high C/N ratio of wheat straw, due to soil microbes assimilating/immobilizing mineralizable N into their biomass and making it temporarily available [32]. According to Parton et al. [32], net immobilization takes place when decomposing material has a low N content, as decomposers seek more N from other substrates, such as broccoli residue N in this case. Thus, in +CR+S and +CR+Sw, the N content of broccoli residue might have been utilized by microbes to decompose straw which otherwise could have been lost through denitrification or other processes. Furthermore, high lignin content (43.9%) in wheat straw [33] could also be responsible for reduced N 2 O emissions in the straw-amended soil [34]. A negative relationship between lignin content and the mineralization rate has been well-documented in several past research studies [35][36][37]. A possible reason for the retardation of N mineralization is the production of low molecular weight polyphenol following lignin degradation, which has tendency to combine with organic N compounds to form a resistant complex [38].
Nitrous oxide flux from +CR+Sw was significantly lower than +CR+S only on the first sampling day or 24 h after incubation and showed a general lower trend of emissions for the next two sampling days. This indicated that washing steps might have intensified the immobilization potential of wheat straw in the early stages of the incubation study by removing hot water-leachable C and N from the straw surface, as soil microbes might have assimilated more N mineralized from broccoli residue and from inherent SMN. However, +CR+Sw treatment did not lower cumulative N 2 O emission significantly when compared to +CR+S treatment. To truly understand the fate of washed straw on reducing N 2 O emissions in early stages of decomposition and its role on maximizing overall N immobilization, a short-term incubation trial with more frequent sampling, as previously suggested by Chaves et al. [30], would be needed to clearly elucidate the potential of washed straw on reducing N 2 O emissions in the early stages of incubation.

Effect of Residue Application on CO 2 Emissions
Carbon dioxide emissions peaked within the first few days of crop residue amendment. Elevated CO 2 indicated that heterotrophic denitrification was a source of N 2 O emissions in this study. During microbial degradation of plant residue, soil microbes consume O 2 and respire CO 2 , thereby creating an anoxic environment in the soil system favorable for denitrification [39]. The overall decomposition of residue-amended soil followed a biphasic model used by several researchers [40][41][42]. This model assumes rapid decomposition of an easily degradable fraction followed by the start of the slow decomposition phase [43]. Initially high CO 2 peak on +CR, +CR+S and +CR+Sw treatments (but not in +S) would confirm a large amount of easily available C pool on +CR [44]. While the decline in CO 2 emissions without any intermediate peaks on +CR could be related to substrate exhaustion, small intermediate CO 2 peaks on +CR+S, +CR+Sw and +S which occurred throughout the incubation period could be related to successive decomposition of compounds that were previously resistant to decomposition. Residue fraction such as fats, waxes, resins and oils are utilized once readily soluble fractions including sugars, starch, proteins and organic acids are used up [20,45]. Additionally, the slow nature of the microorganism [46] involved in the later stage could be the cause for a lower maxima of CO 2 evolution and the time lag between two maxima.

Experimental Soil
The soil used in this study was silt loam (haplic luvisol derived from periglacial loess) and collected from Heidfeldhof, an experimental farm of the University of Hohenheim, Germany (48 • 43 00" N latitude and 9 • 11 40" E). The stone-free topsoil contained 2% clay, 68% silt and 30% sand with an initial pH (10 −2 M CaCl 2 ) of 6.5.

Broccoli Residue and Straw Preparation
Shredded broccoli residues were dried and cracked into small pieces before application. Wheat straw (chopped into 2-3 cm length) was immersed in hot (70 • C) distilled water and shaken vigorously, then the leachate was drained. A similar process was repeated for seven times, preserving the leachate sample each time for laboratory analysis. The final leachate contained <32 mg C L −1 and <1 mg N L −1 , which represents 6% less C and 71% less N than the first leachate sample. Chemical constituents of broccoli crop residue and wheat straw used as amendment materials are shown in Table 3.

Soil Microcosm and Experiment Set-Up
The soil microcosm system comprised a cylindrical Plexiglas core (inner diameter = 13 cm, height = 20 cm), a thick broad bottom plate with central hole to provide outlet of percolate water and a Plexiglas lid with an irrigation nozzle on the top and fittings for inlet and outlet for gas exchange on the opposite side ( Figure 4). Circular rubber seals were installed in between the bottom plate, the Plexiglas lid connected three parts and the screw running through made the system airtight.
Nitrogen 2021, 2, 7 Table 3. Chemical constituents of crop residue used in this incubation study.

Soil Microcosm and Experiment Set-Up
The soil microcosm system comprised a cylindrical Plexiglas core (inner diameter = 13 cm, height = 20 cm), a thick broad bottom plate with central hole to provide outlet of percolate water and a Plexiglas lid with an irrigation nozzle on the top and fittings for inlet and outlet for gas exchange on the opposite side ( Figure 4). Circular rubber seals were installed in between the bottom plate, the Plexiglas lid connected three parts and the screw running through made the system airtight. During packing of soil into microcosms, the surface of the bottom plate was covered with cellulose acetate filter paper (pore size 0.45 µm, Sartorius, Göttingen, Germany) that separated the percolate water from the soil system. Individual microcosms were packed up to 15 cm with 2703 g soil and irrigated to adjust to 60% WFPS with a bulk density of 1.2 g cm −3 . Soil microcosms were incubated in the climate chamber at a constant temperature of 20 °C. Each microcosm was irrigated at 25 mL per day through an irrigation nozzle connected to a pressure cylinder filled with irrigation solution (0.01 M CaCl2). The partial pressure of 10 kPa maintained at the bottom ensured continuous percolation. The microcosms system was flushed with ambient air supply on the headspace at a flow rate of 15 mL min −1 for the whole experimental period. The flow rate was adjusted by a high precision digital flow meter (Thermo Scientific, Langenselbold, Germany).
Soil microcosms were pre-incubated to stabilize their respiration and gas fluxes while feeding them with irrigation solution until soil nitrate contents reached comparable low levels. A quick test was performed to estimate nitrate content of percolate every two days by using a reflectometer Nitracheck (Hermann Wolf Ltd., Wuppertal, Germany). After a During packing of soil into microcosms, the surface of the bottom plate was covered with cellulose acetate filter paper (pore size 0.45 µm, Sartorius, Göttingen, Germany) that separated the percolate water from the soil system. Individual microcosms were packed up to 15 cm with 2703 g soil and irrigated to adjust to 60% WFPS with a bulk density of 1.2 g cm −3 . Soil microcosms were incubated in the climate chamber at a constant temperature of 20 • C. Each microcosm was irrigated at 25 mL per day through an irrigation nozzle connected to a pressure cylinder filled with irrigation solution (0.01 M CaCl 2 ). The partial pressure of 10 kPa maintained at the bottom ensured continuous percolation. The microcosms system was flushed with ambient air supply on the headspace at a flow rate of 15 mL min −1 for the whole experimental period. The flow rate was adjusted by a high precision digital flow meter (Thermo Scientific, Langenselbold, Germany).
Soil microcosms were pre-incubated to stabilize their respiration and gas fluxes while feeding them with irrigation solution until soil nitrate contents reached comparable low levels. A quick test was performed to estimate nitrate content of percolate every two days by using a reflectometer Nitracheck (Hermann Wolf Ltd., Wuppertal, Germany). After a quick test revealed the removal of a considerable amount of nitrate from the soil, microcosms were fertigated with 0.01 M CaCl 2 and 4.1 mg (NH 4 ) 2 NO 3 L −1 solution. This soil nitrate content represents a typical N amount (50 kg N ha −1 ) after broccoli crop harvest.

Experimental Design
The experiment was carried out as a randomized complete block design with five treatments and four replications in July/August 2015. The treatments used were unamended or control (BS), wheat straw (+S), broccoli residue (+CR), broccoli residue and wheat straw (+CR+S) and broccoli residue and washed wheat straw (+CR+Sw). The schematic diagram of the experimental design is shown in Figure 5.

Experimental Design
The experiment was carried out as a randomized complete block design with five treatments and four replications in July/August 2015. The treatments used were unamended or control (BS), wheat straw (+S), broccoli residue (+CR), broccoli residue and wheat straw (+CR+S) and broccoli residue and washed wheat straw (+CR+Sw). The schematic diagram of the experimental design is shown in Figure 5. During the residue amendments into the microcosms, soil was scooped out from the microcosm up to a depth of 7 cm and mixed with respective treatments. Broccoli residue and wheat straw were incorporated at 3.5 kg fresh matter m −2 and 2 kg fresh matter m −2 , respectively. The mixture was sieved (4 mm) to maintain soil aggregation and it was finally placed back in the climate chamber and incubated for 14 days with respected tube fittings.

Gas Measurement
Gas samples from the microcosms were obtained every 12 h by inserting a vial (ambient pressure) to the respective outlet tubing system, which was fed by the headspace gas. Glass vials (22.4 mL) equipped with crimp lock and isobutyl plugs were used for all gas samplings. Gas samples thus collected from individual microcosms were analyzed for their N2O and CO2 concentration using gas chromatography (GC 456 greenhouse gas analyzer, Bruker Daltonic) with a 63 Ni electron capture detector. Raw gas flux measurements thus obtained from gas chromatograph were expressed in μg m −2 h −1 with following equation:
During the residue amendments into the microcosms, soil was scooped out from the microcosm up to a depth of 7 cm and mixed with respective treatments. Broccoli residue and wheat straw were incorporated at 3.5 kg fresh matter m −2 and 2 kg fresh matter m −2 , respectively. The mixture was sieved (4 mm) to maintain soil aggregation and it was finally placed back in the climate chamber and incubated for 14 days with respected tube fittings.

Gas Measurement
Gas samples from the microcosms were obtained every 12 h by inserting a vial (ambient pressure) to the respective outlet tubing system, which was fed by the headspace gas. Glass vials (22.4 mL) equipped with crimp lock and isobutyl plugs were used for all gas samplings. Gas samples thus collected from individual microcosms were analyzed for their N 2 O and CO 2 concentration using gas chromatography (GC 456 greenhouse gas analyzer, Bruker Daltonic) with a 63 Ni electron capture detector. Raw gas flux measurements thus obtained from gas chromatograph were expressed in µg m −2 h −1 with following equation:

Statistical Analysis
The statistical analysis was performed using SAS (SAS 9.4). The N 2 O emissions data were, respectively, y = sqrt(y + 1) and y = log(y + 1), transformed (back-transformed at the end) to achieve normal distribution for statistical analysis. Statistical significance was evaluated at p < 0.05 unless otherwise stated. Percentage reduction in cumulative emissions from +CR+S and +CR+Sw treatments relative to emissions in broccoli was calculated by subtracting cumulative N 2 O emissions of broccoli from +CR+S and +CR+Sw treatments, respectively, and dividing this by N 2 O emissions from the +CR treatment. Similarly, N 2 O emission factor (EF) was calculated according to Velthof

Conclusions
In the current study, +CR amendment showed higher N 2 O emissions than the other treatments, and the effect of simultaneous incorporation of broccoli residue with wheat straw or washed wheat straw on reducing N 2 O emissions relative to broccoli residue single amendment was also clear. When compared to +CR treatment, N 2 O emission in +CR+S and +CR+Sw was reduced by 73.3% and 74.2%, respectively. However, the effect of washed wheat straw with broccoli residue mixture amendment on reducing N 2 O fluxes (when compared to broccoli residue and unwashed straw) was evident only for the first few sampling days and the trend was rather elusive. Therefore, future field studies in vegetable production should also focus on the effect of N 2 O reduction through straw application over the long-term, as well as on environmental hazards through the release of the transient immobilized mineral N.

Data Availability Statement:
The data presented in this study are available on request from the corresponding author.