Discovery of X-rays—Its Impact in India and History of X-ray Research in Colonial India

: India holds a respectable position globally in X-ray research, particularly in X-ray crystallography. X-ray research in India is as old as the discovery of X-rays and the history of X-ray research in colonial India is fascinating. The purpose of this paper is to present how India participated in X-ray research and how X-ray research initiated by C.V. Raman, the only Indian Nobel Laureate in physics, at the Indian Association for the Cultivation of Science (IACS) paved the way to proliferate X-ray research in all parts of India and acted as the foundation stone of modern X-ray research in India. With limited resources under the British rule (India became independent in 1947), readers will ﬁnd that the research work performed by Indians is commendable. This article is neither comprehensive nor detailed but will give the readers a ﬂavour of the high-quality X-ray research that was performed in India in the early years after the discovery of X-rays.


Introduction
India entered the international mainstream of scientific research in the late nineteenth and early twentieth century. It was Sir Jagadish Chandra Bose (1858Bose ( -1937 who first showed the world that it is possible to do science at par with Western standards in colonial India when he demonstrated his millimetre wave experiment with equipment made in India with limited resources before the Royal Society of London during his first scientific mission to Europe (1896-1897). Serious research began in universities in the 1920s and 1930s in Allahabad, Dhaka (now in Bangladesh), Punjab, Delhi, Mysore, Andhra Pradesh, Banaras, etc., pursuing the idea of teaching-cum-research institutions in contrast to the purely teaching universities set up by the British [1].
So far as X-ray research in India is concerned, it started immediately after the discovery of X-rays first by excitation to reproduce X-ray photographs indigenously and then to pursue research more seriously [2,3]. C.V. Raman started his scientific research as an amateur at Indian Association in Calcutta and later turned into a serious researcher cum teacher and earned the Nobel Prize in physics in 1930. After his return from England in 1921, C.V. Raman started serious scientific research using X-rays with a host of talented students which paved the way for building X-ray crystallography research centres across India.

Discovery of X-rays
Wilhelm Conrad Roentgen (1845-1923) has been credited with the discovery of X-rays and was awarded the first Nobel Prize in Physics in 1901 for this discovery. Roentgen's discovery is known to be the result of an accident while he was experimenting with a discharge tube. The objective of the current article is to present a historical account of X-ray discoveries, such as that X-rays were produced experimentally much before Roentgen's discovery in 1895 as has been found in literature and to study the influence of this discovery on the scientific community of India.
The twenty-eighth of December 1895 is considered as the date of discovery of X-ray, the date on which Roentgen submitted his first "provisorial" communication Uebereineneue The twenty-eighth of December 1895 is considered as the date of discovery of X-ray, the date on which Roentgen submitted his first "provisorial" communication Uebereineneue Art von Strahlen (On a New Kind of Rays) which was published in the Proceedings of the Würzburg Physico-Medical Society (Sitzungber der WürzburgerPhysik.- MedikGesellschaft). First oral presentation of the discovery was made before the same Würzburg Society on 23 January 1896. The meeting was chaired by the famous anatomist Albert Rudolf von Kolliker (1817Kolliker ( -1905 as reported in the Münchener Medicinische Wochenschrift of 29 January 1896. After the lecture Roentgen produced an X-ray picture of Kolliker's hand in a glass plate (Figure 1). It was Dr. Kolliker who proposed that the new rays henceforth be known as Roentgen rays [4]. Roentgen's discovery was shrouded with mystery, stories, and controversies which sometimes reached a stage of humiliation to the great scientist. It is believed that fluorescence was first noticed by Roentgen's laboratory assistant Ludwig Zhender in 1890, who, while working with the discharge tube covered with black cloth, observed the fluorescence in the fluorescent screen placed nearby [5]. Zhender was loyal to Roentgen throughout his life and had not claimed any recognition, but this story was severely distorted to humiliate Roentgen and he was accused of stealing his Diener's discovery [4]. It is to be remembered in this connection that X-ray tubes that we are talking about were known as cold discharge tubes in which ionization of gas by high voltage was the source of electrons in contrast to modern X-ray tubes which contain a heated filament for producing electrons.
According to Roentgen's will, all his diaries and notes were destroyed after his death which resulted in a great loss of vital information for historians, such as the exact date when Roentgen realized the importance of the penetrating power of this new ray. From a letter written by his wife Anna Bertha Ludwig in March 1896 to one of his cousins Mrs. L.R. Grauel of Indianapolis, it was found that Roentgen first noticed this new radiation some time in November 1895 [4]. As Bertha mentioned in her letter, on an evening of November 1895 she became angry with her husband for the quality of food. In order to soothe Roentgen's discovery was shrouded with mystery, stories, and controversies which sometimes reached a stage of humiliation to the great scientist. It is believed that fluorescence was first noticed by Roentgen's laboratory assistant Ludwig Zhender in 1890, who, while working with the discharge tube covered with black cloth, observed the fluorescence in the fluorescent screen placed nearby [5]. Zhender was loyal to Roentgen throughout his life and had not claimed any recognition, but this story was severely distorted to humiliate Roentgen and he was accused of stealing his Diener's discovery [4]. It is to be remembered in this connection that X-ray tubes that we are talking about were known as cold discharge tubes in which ionization of gas by high voltage was the source of electrons in contrast to modern X-ray tubes which contain a heated filament for producing electrons.
According to Roentgen's will, all his diaries and notes were destroyed after his death which resulted in a great loss of vital information for historians, such as the exact date when Roentgen realized the importance of the penetrating power of this new ray. From a letter written by his wife Anna Bertha Ludwig in March 1896 to one of his cousins Mrs. L.R. Grauel of Indianapolis, it was found that Roentgen first noticed this new radiation some time in November 1895 [4]. As Bertha mentioned in her letter, on an evening of November 1895 she became angry with her husband for the quality of food. In order to soothe her, Roentgen took her downstairs to his laboratory and introduced her to the mysterious new rays. However, it was not mentioned whether her hand with a ring was exposed to this ray ( Figure 2) on the same day. According to Edgar Ashworth Underwood, Director of her, Roentgen took her downstairs to his laboratory and introduced her to the mysterious new rays. However, it was not mentioned whether her hand with a ring was exposed to this ray ( Figure 2) on the same day. According to Edgar Ashworth Underwood, Director of Wellcome Historical Museum, the picture of his wife's hand was taken on November 8, 1895. This date was accepted by Konrad Weiss, the historiographer of Austrian Roentgen Society and is accepted as the date of the discovery. Like Jagadish Chandra Bose (1858-1937) who refused to take patent of his discovery of microwave (the discovery for which Guglielimo Marconi was awarded the Nobel Prize) [6], on the ground that knowledge is for all and should be freely available, Roentgen also refused to take patent of any part of his discovery and rejected all commercial proposals connected to this discovery. However, unlike J. C. Bose, he lost all his savings due to post war inflation and suffered financial and other difficulties in the last years of his life. Roentgen died in 1923 from colorectal cancer.

Production of X-rays before Roentgen
There is evidence that X rays had been produced experimentally before Roentgen's date in 1895. This is not surprising because the basic physical process to generate X rays is the passage of electricity through gases, the study of which had been started in eighteenth century. Francis Hauksbee (estimated to have died in 1713) who was the Curator of Experiments to the Royal Society, London, described seeing "the shape and figure of all parts of his hand" while working with electricity and vacuum [5]. Interest in the study of discharge in gases was revived in the middle of nineteenth century after Faraday's experiments with "radiant matter" and many scientists were involved in producing different improvised discharge tubes to study discharge of gases. Heinrich Geissler, Johann Wilhelm Hittroff, and Sir William Crookes were some of the people who worked with discharge tubes and they were unknowingly exposed to X-rays while working.
In India, Father Lafont (1837-1908), a Jesuit missionary of St. Xavier's College, Calcutta brought from Europe a Crooke's tube at a time (1878-1879) when vigorous research was in progress in Europe using Crooke's tube. Father Lafont delivered a lecture in 1880 Like Jagadish Chandra Bose (1858-1937) who refused to take patent of his discovery of microwave (the discovery for which Guglielimo Marconi was awarded the Nobel Prize) [6], on the ground that knowledge is for all and should be freely available, Roentgen also refused to take patent of any part of his discovery and rejected all commercial proposals connected to this discovery. However, unlike J. C. Bose, he lost all his savings due to post war inflation and suffered financial and other difficulties in the last years of his life. Roentgen died in 1923 from colorectal cancer.

Production of X-rays before Roentgen
There is evidence that X-rays had been produced experimentally before Roentgen's date in 1895. This is not surprising because the basic physical process to generate X-rays is the passage of electricity through gases, the study of which had been started in eighteenth century. Francis Hauksbee (estimated to have died in 1713) who was the Curator of Experiments to the Royal Society, London, described seeing "the shape and figure of all parts of his hand" while working with electricity and vacuum [5]. Interest in the study of discharge in gases was revived in the middle of nineteenth century after Faraday's experiments with "radiant matter" and many scientists were involved in producing different improvised discharge tubes to study discharge of gases. Heinrich Geissler, Johann Wilhelm Hittroff, and Sir William Crookes were some of the people who worked with discharge tubes and they were unknowingly exposed to X-rays while working.
In India, Father Lafont (1837-1908), a Jesuit missionary of St. Xavier's College, Calcutta brought from Europe a Crooke's tube at a time (1878-1879) when vigorous research was in progress in Europe using Crooke's tube. Father Lafont delivered a lecture in 1880 titled "Crookes on Radiant Energy" in the Science Association [7]. Lord Lytton, the then Viceroy of India, invited Dr. Mahendralal Sircar (1833-1904), the founder of Indian Association for the Cultivation of Science (IACS), to demonstrate the actions of Crookes tube. A contemporary report described: "It is not possible for any individual to forget the evening. Dr. Sircar had such a wonderful mastery over the subject that he very easily explained the amazing behaviour of one millionth of atmosphere to the entire satisfaction to His excellency".
It has been said that Jagadish Chandra Bose built an X-ray apparatus (presumably used a discharge tube) a few years before Roentgen and demonstrated "X-rays passing through the hand" [7]. As reported by A.K. Biswas [8] "we have reports of Jagadish setting up his own X-ray apparatus in 1887, quite a few years before such a machine was imported to India". Therefore, we see that X-ray research in India had its root before the formal announcement of the discovery of X-rays.
The first recorded scholarly evidence to produce Roentgen rays was about hundred years before Roentgen by William Morgan (1750-1833), the Welsh mathematician and Chief Actuary of British Equitable Assurance Society. Morgan [9] reported in the Philosophical Transactions that based on the length of time for which mercury was boiled in vacuum-the 'electric' light turned violet, purple, then beautiful green, and finally the light became invisible! Morgan presented this discovery to the Royal Society in 1784 which was witnessed by Richard Price and one of Richard's friends, an American 'electrician', the famous Benjamin Franklin (1706-1790).
It is believed that Philipp Lenard (1862-1947), another contemporary of Roentgen, who worked on cathode rays came very close to the discovery of X-rays. In fact, both Lenard and Roentgen were nominated for the Nobel Prize in Physics for the year 1901, and the Committee recommended that the prize should be divided equally between Roentgen and Lenard. However, the Royal Academy of Science did not follow this recommendation and decided to award the prize to Roentgen alone. On 12 October 1892, Lenard had been able to show for the first time that cathode rays can penetrate the aluminium window and can travel a few inches in air by observing the fluorescence produced on granules of potassium phosphate placed outside the tube [10]. Four years later, in 1905, the Nobel Committee decided to award the Nobel Prize to Lenard for his ingenious work on cathode rays. Lenard, however, considered himself to be "the mother of X-rays" while Roentgen was "the midwife who happened to deliver the child". There are other scientists who are believed to have observed X-rays before Roentgen and we are not going into the details of those stories.

The First X-ray Photograph: Claims and Counter-Claims
The very first terms used for pictures produced by X-rays were the unabbreviated and easily understandable combination of words such as 'X-ray photograph' or 'shadow photograph'. There were a lot of debates, controversies, personal choices, and etymological discussions in choosing the name of the pictures taken by X-rays [3].
Who first produced the first X-ray photograph after the discovery of X-rays is replete with claims and counter-claims. It is said that the first shadow graph or Roentgenograph of metal objects [4] was obtained by accident in 1892 by a Philadelphia experimentalist Arthur Willis Goodspeed (1860-1943). The story goes that Bill Jennings, a friend of Goodspeed, was counting his coins after the Bill Jennings' Carfare on the Woodland Avenue trolley when Goodspeed asked Jennings to help in photographing the spark gap of a Ruhmkorff coil. Jennings put his coins on the top of a plate while assisting in the positioning of the electrodes. The plate was later found to have black patches. Rounded shadows shown in Figure 3 are the images of coins. They paid little or no attention to those circular shadows till the announcement of Roentgen's discovery. They then repeated the experiment and understood the reason. However, Goodspeed expressly and repeatedly denied any claims to priority because at that time he had failed to interpret these shadows. Quantum Beam Sci. 2022, 6, x FOR PEER REVIEW 5 of 11 The most legitimate claim was that of a Scottish engineer Alan Archibald Campbell Swinton (1863-1930) who tried to replicate X-ray photos in the beginning of 1896 immediately after the discovery [4]. He conducted his experiment using a homemade tube and his plates were first reproduced in Nature and subsequently in Industries and Iron and the Electrical Review of London the next day [11]. From the reconstructed timetable that he maintained, the following were captured: first (poor) roentgenogram on Tuesday January 7; first satisfactory "metallic" (a razor in its paperboard casing) roentgenogram on January 8; first roentgenogram of a hand on January 13 (shown to the Prince of Wales); and first exhibit of those two plates on 16 January 1896.
Michael Idvorsky Pupin (1858-1935) of USA, an immigrant from former Serbia, claimed that he produced the first roentgenogram on 2 January 1896 after Roentgen. In 1924, he wrote: "I obtained the first X-ray photograph in America on 2 January 1896, two weeks after the discovery was announced in Germany". With the aid of a phosphorescent screen, supplied by his friend Thomas A Edison, superimposed on a photographic plate, he was able to produce a good X-ray picture in a few seconds of exposure [12]. Literature search shows that Pupin's first paper on Roentgen rays (dated Saturday, 1 February 1896) was published on February 5 in Electricity of New York which was essentially a summary of data from European sources [4]. His first roentgen plate, as mentioned in his article, was published in Science around February 14.
Thomas Edison (1847-1931) who is famous for his invention of electric bulb, realized the importance of the Roentgen rays as soon as the news of the discovery reached the USA. According to a statement made by his secretary William Henry Meadowcraft, "Mr. Edison was the first to recognize the importance of the cable announcement of Dr. Roentgen's discovery. The same day he started to make the apparatus and had it finished the next day. Three of the metropolitan dailies heard of it and for three weeks more than twenty newspaper reporters were stationed at the Laboratory". The first interview of Edison was made on 7 February 1896 and published in Times on February 8. Edison strongly believed that some practical (commercial) application would emerge from Roentgen's "purely scientific" discovery, and he employed his staff to find out the most favourable conditions for taking roentgen photograph. A sketch of the design of one of his earliest apparatuses using Hittrof tube is presented in Figure 4. The most legitimate claim was that of a Scottish engineer Alan Archibald Campbell Swinton (1863-1930) who tried to replicate X-ray photos in the beginning of 1896 immediately after the discovery [4]. He conducted his experiment using a homemade tube and his plates were first reproduced in Nature and subsequently in Industries and Iron and the Electrical Review of London the next day [11]. From the reconstructed timetable that he maintained, the following were captured: first (poor) roentgenogram on Tuesday January 7; first satisfactory "metallic" (a razor in its paperboard casing) roentgenogram on January 8; first roentgenogram of a hand on January 13 (shown to the Prince of Wales); and first exhibit of those two plates on 16 January 1896.
Michael Idvorsky Pupin (1858-1935) of USA, an immigrant from former Serbia, claimed that he produced the first roentgenogram on 2 January 1896 after Roentgen. In 1924, he wrote: "I obtained the first X-ray photograph in America on 2 January 1896, two weeks after the discovery was announced in Germany". With the aid of a phosphorescent screen, supplied by his friend Thomas A Edison, superimposed on a photographic plate, he was able to produce a good X-ray picture in a few seconds of exposure [12]. Literature search shows that Pupin's first paper on Roentgen rays (dated Saturday, 1 February 1896) was published on February 5 in Electricity of New York which was essentially a summary of data from European sources [4]. His first roentgen plate, as mentioned in his article, was published in Science around February 14.
Thomas Edison (1847-1931) who is famous for his invention of electric bulb, realized the importance of the Roentgen rays as soon as the news of the discovery reached the USA. According to a statement made by his secretary William Henry Meadowcraft, "Mr. Edison was the first to recognize the importance of the cable announcement of Dr. Roentgen's discovery. The same day he started to make the apparatus and had it finished the next day. Three of the metropolitan dailies heard of it and for three weeks more than twenty newspaper reporters were stationed at the Laboratory". The first interview of Edison was made on 7 February 1896 and published in Times on 8 February. Edison strongly believed that some practical (commercial) application would emerge from Roentgen's "purely scientific" discovery, and he employed his staff to find out the most favourable conditions for taking roentgen photograph. A sketch of the design of one of his earliest apparatuses using Hittrof tube is presented in Figure 4. Quantum Beam Sci. 2022, 6, x FOR PEER REVIEW 6 of 11

X-ray Discovery and Its Impact in India
It is not known exactly when and how the news of X-ray discovery reached Calcutta (now Kolkata), but it is fascinating to note that it had produced huge interest among the science lovers of colonial India. Within a few months of the discovery of X-rays, Dr. Mahendralal Sircar (MLS) ordered a Roentgen tube from Ducretet Company in Europe and received it in June 1896. In his diary dated 11 June 1896 we find the following "The cases (3 in number) from Ducretet containing apparatus for experimenting with Roentgen Rays were brought from the Customs House to the Association today. When Amrita (son of Mahendralal Sircar) opened them, he found that they have omitted to send the Flourescent Screen. The cathode disc was slightly bent. The cells were too big" [8].
The first experiment using X-rays in India was carried out by Mahendralal Sircar (MLS) on 20 June 1896 by taking the photograph of a hand using the procured Roentgen's apparatus and it was noted in his diary that he did not obtain a good picture in his first attempt, probably due to over exposure. As noted in his diary, he repeated his experiment to obtain a better photograph and was successful on 23 June. Therefore, according to his diary, the first successful X-ray photograph was produced in India on 23 June 1896 in Calcutta by Mahendralal Sircar [13].
Experiments with X-rays were continued at the IACS by Amrita Lal Sircar under the guidance of MLS with blocks of wood and books of different thickness, with sheets of iron, tin foil and zinc foil as has been recorded in the diary dated 13 December 1899 [4]. This was the beginning of X-ray research in India after the discovery of X-rays and thus IACS has the distinction of introducing X-ray research in India.
A spurt of activities continued in India when people were trained in X-ray photography from England and there is evidence of obtaining an excellent photograph of the right hand of Earl of Elgin, the then Viceroy of India, wearing rings [13].

J. C. Bose and His X-ray Apparatus
Acharya Jagadish Chandra Bose (1858-1937) ( Figure 5) is known for his discovery of microwaves [14] and his epoch-making work on plant physiology demonstrating that plants respond to external stimuli in a way very similar to animals [15][16][17]. However, what is less known is that he was the first person in India who built an improved Roentgen's apparatus on his return from England in 1897. Acharya Bose visited Europe in 1896 at a time when the excitement of the discovery of Roentgen rays in Europe was at its peak. It is possible that Jagadish Chandra, being a physicist and an exceptional experimentalist,

X-ray Discovery and Its Impact in India
It is not known exactly when and how the news of X-ray discovery reached Calcutta (now Kolkata), but it is fascinating to note that it had produced huge interest among the science lovers of colonial India. Within a few months of the discovery of X-rays, Dr. Mahendralal Sircar (MLS) ordered a Roentgen tube from Ducretet Company in Europe and received it in June 1896. In his diary dated 11 June 1896 we find the following "The cases (3 in number) from Ducretet containing apparatus for experimenting with Roentgen Rays were brought from the Customs House to the Association today. When Amrita (son of Mahendralal Sircar) opened them, he found that they have omitted to send the Flourescent Screen. The cathode disc was slightly bent. The cells were too big" [8].
The first experiment using X-rays in India was carried out by Mahendralal Sircar (MLS) on 20 June 1896 by taking the photograph of a hand using the procured Roentgen's apparatus and it was noted in his diary that he did not obtain a good picture in his first attempt, probably due to over exposure. As noted in his diary, he repeated his experiment to obtain a better photograph and was successful on 23 June. Therefore, according to his diary, the first successful X-ray photograph was produced in India on 23 June 1896 in Calcutta by Mahendralal Sircar [13].
Experiments with X-rays were continued at the IACS by Amrita Lal Sircar under the guidance of MLS with blocks of wood and books of different thickness, with sheets of iron, tin foil and zinc foil as has been recorded in the diary dated 13 December 1899 [4]. This was the beginning of X-ray research in India after the discovery of X-rays and thus IACS has the distinction of introducing X-ray research in India.
A spurt of activities continued in India when people were trained in X-ray photography from England and there is evidence of obtaining an excellent photograph of the right hand of Earl of Elgin, the then Viceroy of India, wearing rings [13].

J. C. Bose and His X-ray Apparatus
Acharya Jagadish Chandra Bose (1858-1937) ( Figure 5) is known for his discovery of microwaves [14] and his epoch-making work on plant physiology demonstrating that plants respond to external stimuli in a way very similar to animals [15][16][17]. However, what is less known is that he was the first person in India who built an improved Roentgen's apparatus on his return from England in 1897. Acharya Bose visited Europe in 1896 at a time when the excitement of the discovery of Roentgen rays in Europe was at its peak. It is possible that Jagadish Chandra, being a physicist and an exceptional experimentalist, was attracted to this discovery and studied in detail about the production of Roentgen rays. was attracted to this discovery and studied in detail about the production of Roentgen rays. Jagadish Chandra Bose returned to India in April 1897 and started building his apparatus [3]. D M Bose, his nephew, and former Director of Bose Institute, remarked in one of his articles that after 'reading a newspaper account of Roentgen's discovery' he built an X-ray apparatus in Presidency College, Calcutta [18].
The exact date of his building the apparatus is not known but it appears to have been built sometime around 1897-1898. We found the first mention of his X-ray apparatus in a letter written to Rabindranath Tagore, the first Asian to receive the Nobel Prize in literature, in February 1898 [19]. Unfortunately, neither the apparatus nor any sketch of his apparatus is available either at Presidency College, Calcutta, the place where he built the apparatus or at Bose Institute, Calcutta, the institute that he built later, to understand the mechanism of the apparatus. Fortunately, we have been able to find a Press Report published on the 5th of May 1898 edition of the Amrita Bazar Patrika, an erstwhile reputed English daily of India, under the title 'Professor Bose and the New Light' which describes the X-ray apparatus built by him and its demonstration.
Roentgen used Ruhmkorff's coil as a source of transient high voltage in discharging the gas in Crooke's tube. Ruhmkorff's coil produces high voltage pulse in the secondary coil by electromagnetic induction every time when a dc supply in the primary is interrupted by mechanical means. The secondary is connected with the discharge tube (X-ray tube) and the high voltage pulse was enough to produce X-rays. By coupling a Tesla coil with the secondary of Ruhmkorff's coil (which then acts as the primary of the tesla transformer).
Jagadish Chandra had been able to produce a higher voltage many times more than that can be produced using a single Ruhmkorff's coil. On the basis of the published information, we have presented a schematic diagram ( Figure 6) to represent the apparatus he had probably used. The newspaper reported "We were shown a photograph of human palm taken by the Professor with the new light, and the ghastly sight will long be vividly imprinted in our memory, for there, in the photograph, instead of the ordinary fleshy palm is seen depicted a long range of bones presenting a skeleton-like appearance." Using Barium Platinocyanide screen prepared by himself with his assistants in the Presidency College, he took X-ray photographs of different objects. Jagadish Chandra Bose returned to India in April 1897 and started building his apparatus [3]. D M Bose, his nephew, and former Director of Bose Institute, remarked in one of his articles that after 'reading a newspaper account of Roentgen's discovery' he built an X-ray apparatus in Presidency College, Calcutta [18].
The exact date of his building the apparatus is not known but it appears to have been built sometime around 1897-1898. We found the first mention of his X-ray apparatus in a letter written to Rabindranath Tagore, the first Asian to receive the Nobel Prize in literature, in February 1898 [19]. Unfortunately, neither the apparatus nor any sketch of his apparatus is available either at Presidency College, Calcutta, the place where he built the apparatus or at Bose Institute, Calcutta, the institute that he built later, to understand the mechanism of the apparatus. Fortunately, we have been able to find a Press Report published on the 5th of May 1898 edition of the Amrita Bazar Patrika, an erstwhile reputed English daily of India, under the title 'Professor Bose and the New Light' which describes the X-ray apparatus built by him and its demonstration.
Roentgen used Ruhmkorff's coil as a source of transient high voltage in discharging the gas in Crooke's tube. Ruhmkorff's coil produces high voltage pulse in the secondary coil by electromagnetic induction every time when a dc supply in the primary is interrupted by mechanical means. The secondary is connected with the discharge tube (X-ray tube) and the high voltage pulse was enough to produce X-rays. By coupling a Tesla coil with the secondary of Ruhmkorff's coil (which then acts as the primary of the tesla transformer).
Jagadish Chandra had been able to produce a higher voltage many times more than that can be produced using a single Ruhmkorff's coil. On the basis of the published information, we have presented a schematic diagram ( Figure 6) to represent the apparatus he had probably used. The newspaper reported "We were shown a photograph of human palm taken by the Professor with the new light, and the ghastly sight will long be vividly imprinted in our memory, for there, in the photograph, instead of the ordinary fleshy palm is seen depicted a long range of bones presenting a skeleton-like appearance." Using Barium Platinocyanide screen prepared by himself with his assistants in the Presidency College, he took X-ray photographs of different objects. Although use of X-rays in India was started within a few months of X-ray discovery by importing X-ray tube from abroad, commercial production of indigenous machine made in India started only a few years before India's independence in 1947 [3].

Medical Uses of X-rays
As per available literature, the evidence of first clinical uses of X-rays in India, at least in an informal setting, was started by Jagadish Chandra Bose in 1898. In a letter (Sen, 1994) written possibly in February 1898 to Rabindranath Tagore, he expressed his inability to meet Rabindranath at a time when he will be busy examining a patient with a broken back using Roentgen kal (machine in Bengali) [19]. The history of application of X-rays by professionals for clinical purposes in India is not very clearly known. As reported [20], Dr. Kartick Bose (1873Bose ( -1955, an eminent doctor, medical scholar and industrialist was "the first clinician in India to start clinical and X-ray laboratories". Installation of the first X-ray machine in hospitals for public use is full of claims, counter-claims and conflicting reports so much so that it is very difficult to arrive at a definite conclusion [13]. It was reported by K P Mody in one of his editorials published in the Indian Journal of Radiology & Imaging that "the first X-ray machine was imported by a chemist in 1902 into India; that was only 7 years after the discovery" [21]. Madras Medical College website (www.mmc.ac.in (accessed on 17 June 2021)) claimed that the "first X-ray outfit was obtained for the general hospital in the year 1900 almost five years after the discovery of X-rays, the first in South East Asia". This might be true considering that it is a very old medical institution started first as a Government General Hospital on 16 November 1664 to treat sick soldiers of the East India Company.
Use of X-rays for therapeutical purposes was also carried out at the beginning of twentieth century. Successful treatment of leukaemia using X-rays was reported in Calcutta Medical Journal [22] within five years of the first ever treatment of leukaemia, as reported in a paper published in the Journal of the American Medical Association in 1902.
Excitement of using X-rays in medical science waned with time and was replaced by Xray research in physical sciences.

C.V. Raman and Initiation of X-ray Research in India
In July 1921, C.V. Raman (Figure 7) on his voyage to England as a delegate of Calcutta and Banaras University to attend University Congress, was attracted to the "mystery of the blue colour of the sea". On his return to India, he undertook a comprehensive programme of research on molecular scattering of light by solid, liquid and gases which led to the discovery of a new light effect. The new light effect was later known as 'Raman Although use of X-rays in India was started within a few months of X-ray discovery by importing X-ray tube from abroad, commercial production of indigenous machine made in India started only a few years before India's independence in 1947 [3].

Medical Uses of X-rays
As per available literature, the evidence of first clinical uses of X-rays in India, at least in an informal setting, was started by Jagadish Chandra Bose in 1898. In a letter (Sen, 1994) written possibly in February 1898 to Rabindranath Tagore, he expressed his inability to meet Rabindranath at a time when he will be busy examining a patient with a broken back using Roentgen kal (machine in Bengali) [19]. The history of application of X-rays by professionals for clinical purposes in India is not very clearly known. As reported [20], Dr. Kartick Bose (1873Bose ( -1955, an eminent doctor, medical scholar and industrialist was "the first clinician in India to start clinical and X-ray laboratories". Installation of the first X-ray machine in hospitals for public use is full of claims, counter-claims and conflicting reports so much so that it is very difficult to arrive at a definite conclusion [13]. It was reported by K P Mody in one of his editorials published in the Indian Journal of Radiology & Imaging that "the first X-ray machine was imported by a chemist in 1902 into India; that was only 7 years after the discovery" [21]. Madras Medical College website (www.mmc.ac.in (accessed on 17 June 2021)) claimed that the "first X-ray outfit was obtained for the general hospital in the year 1900 almost five years after the discovery of X-rays, the first in South East Asia". This might be true considering that it is a very old medical institution started first as a Government General Hospital on 16 November 1664 to treat sick soldiers of the East India Company.
Use of X-rays for therapeutical purposes was also carried out at the beginning of twentieth century. Successful treatment of leukaemia using X-rays was reported in Calcutta Medical Journal [22] within five years of the first ever treatment of leukaemia, as reported in a paper published in the Journal of the American Medical Association in 1902.
Excitement of using X-rays in medical science waned with time and was replaced by Xray research in physical sciences.

C.V. Raman and Initiation of X-ray Research in India
In July 1921, C.V. Raman (Figure 7) on his voyage to England as a delegate of Calcutta and Banaras University to attend University Congress, was attracted to the "mystery of the blue colour of the sea". On his return to India, he undertook a comprehensive programme of research on molecular scattering of light by solid, liquid and gases which led to the discovery of a new light effect. The new light effect was later known as 'Raman effect' and earned him the Nobel Prize in physics in 1930. During this time Raman and his students effect' and earned him the Nobel Prize in physics in 1930. During this time Raman and his students were interested in the study of optical anisotropy of molecules from scattering of light. Raman and Ramanathan [23] extended the optical theory of scattering to X-ray diffraction by liquids. Experiments were also carried out to compare the theory with experimental results. Sogani [24] recorded the X-ray diffraction patterns of 22 aliphatic and aromatic liquids and estimated the average molecular distance from the position of the maxima of the diffraction haloes. It became clear from these experiments that the shape of the molecules and the intermolecular forces had profound influence on the diffraction patterns. Krishnamurti [25], another student of Raman, initiated small angle X-ray scattering which enabled us to understand the size and distribution of the particles in a sample. This is reportedly the first small angle X-ray scattering experiment performed in India [26]. Raman had an encounter with William Bragg and he was of the opinion that Bragg's first structure of naphthalene was not consistent with the birefringence, while the second one was. In order to confirm this, he and his students started extensive studies on the optical and magnetic anisotropy of organic crystals to understand the arrangements of molecules in crystalline state. The work on crystal magnetism was initiated by one of his successful students K.S. Krishnan. In many cases the orientation of the molecules in the unit cell could be calculated with a high degree of precision from purely magnetic data, which helped in refining X-ray analysis of the crystal structure [27,28].
Bidhubhusan Ray, another student of Raman, made significant contribution to X-ray spectroscopy, although he remained unknown to most physicists until a book was published by Rajinder Singh [29]. Bidhubhushan Ray (B. B. Ray) worked with Manne Siegbahn, a Nobel Laureate in Physics, at Uppsala and on his return to India established Xray laboratory at Calcutta University. Satyendranath Bose who visited Europe during 1924-1926 met de Broglie brothers and was attracted to X-ray crystal structure. His experience was utilized both at Dhaka University and later at the Calcutta University where he took charge of the X-ray laboratory after the premature death of B. B. Ray and built an X-ray diffraction apparatus in the laboratory (Figure 8). Raman had an encounter with William Bragg and he was of the opinion that Bragg's first structure of naphthalene was not consistent with the birefringence, while the second one was. In order to confirm this, he and his students started extensive studies on the optical and magnetic anisotropy of organic crystals to understand the arrangements of molecules in crystalline state. The work on crystal magnetism was initiated by one of his successful students K.S. Krishnan. In many cases the orientation of the molecules in the unit cell could be calculated with a high degree of precision from purely magnetic data, which helped in refining X-ray analysis of the crystal structure [27,28].
Bidhubhusan Ray, another student of Raman, made significant contribution to X-ray spectroscopy, although he remained unknown to most physicists until a book was published by Rajinder Singh [29]. Bidhubhushan Ray (B. B. Ray) worked with Manne Siegbahn, a Nobel Laureate in Physics, at Uppsala and on his return to India established X-ray laboratory at Calcutta University. Satyendranath Bose who visited Europe during 1924-1926 met de Broglie brothers and was attracted to X-ray crystal structure. His experience was utilized both at Dhaka University and later at the Calcutta University where he took charge of the X-ray laboratory after the premature death of B. B. Ray and built an X-ray diffraction apparatus in the laboratory (Figure 8). Quantum Beam Sci. 2022, 6, x FOR PEER REVIEW 10 of 11 Historically the first crystallographic activity in India started with the determination of crystal structures of napthaelene and anthracene by Kedareswar Banerjee (KB) at IACS [30,31]. In 1930, in response to KB's article in "Nature" on the structure of naphthalene, J.M. Robertson of Michigan University, Ann Arbor, U.S.A., wrote: "I believe Dr. Banerjee's structure to be essentially correct. It has been clear to me for some time that the last two sections of my paper to which Dr. Banerjee refers must be amended as regards the distribution of the scattering centres in the a and b directions". His further research on crystallography paved the way for a 'complete crystallography structure analysis' and for understanding statistical relationships in the amplitudes of diffracted waves. Further details of his life and work are available in a recently published book [32].
With Raman having good contact with the giants in the field of X-ray crystallography, he was able to send many of his students abroad to let them work with leading scientists of the field to gain contemporary knowledge of the subject. This made IACS the nucleus of X-ray research in India.
After independence, there has been a great surge of scientific research in India through establishment of laboratories by the Govt. of India. X-ray crystallography also flourished and expanded into areas of biological sciences. Contributions of Kedareswar Banerjee were followed by equally profound works by other researchers in India such as G. N. Ramachandran (University of Madras and Indian Institute of Science, Bangalore), A. R. Verma (Banaras Hindu University), S. Ramashesan (National Aeronautical Laboratory, Bangalore), VR. Chidambaram (Bhabha Atomic Research Centre, Mumbai) and others [33].
G.N. Ramachandran, a student of C.V. Raman, and most distinguished crystallographer of independent India worked on fibrous proteins and was recognized for the advancement of triple helix model of the structure of collagen [34] and the Ramachandran plot for validation of protein structure.

Conclusions
India has a legacy of X-ray research since the discovery of X-rays in 1895. Research in physical sciences using X-rays was started in India by C V Raman at the IACS and made significant contribution in colonial India with limited resources available at that time. By virtue of its scientific activity India made a respectable position in X-ray research globally. IACS acted as the nucleus of X-ray research in India from where it proliferated to different laboratories such as the Benares Hindu University, Allahabad University, Madras University, and the Indian Institute of Sciences, Bangalore. Historically the first crystallographic activity in India started with the determination of crystal structures of napthaelene and anthracene by Kedareswar Banerjee (KB) at IACS [30,31]. In 1930, in response to KB's article in "Nature" on the structure of naphthalene, J.M. Robertson of Michigan University, Ann Arbor, U.S.A., wrote: "I believe Dr. Banerjee's structure to be essentially correct. It has been clear to me for some time that the last two sections of my paper to which Dr. Banerjee refers must be amended as regards the distribution of the scattering centres in the a and b directions". His further research on crystallography paved the way for a 'complete crystallography structure analysis' and for understanding statistical relationships in the amplitudes of diffracted waves. Further details of his life and work are available in a recently published book [32].
With Raman having good contact with the giants in the field of X-ray crystallography, he was able to send many of his students abroad to let them work with leading scientists of the field to gain contemporary knowledge of the subject. This made IACS the nucleus of X-ray research in India.
After independence, there has been a great surge of scientific research in India through establishment of laboratories by the Govt. of India. X-ray crystallography also flourished and expanded into areas of biological sciences. Contributions of Kedareswar Banerjee were followed by equally profound works by other researchers in India such as G. N. Ramachandran (University of Madras and Indian Institute of Science, Bangalore), A. R. Verma (Banaras Hindu University), S. Ramashesan (National Aeronautical Laboratory, Bangalore), VR. Chidambaram (Bhabha Atomic Research Centre, Mumbai) and others [33].
G.N. Ramachandran, a student of C.V. Raman, and most distinguished crystallographer of independent India worked on fibrous proteins and was recognized for the advancement of triple helix model of the structure of collagen [34] and the Ramachandran plot for validation of protein structure.

Conclusions
India has a legacy of X-ray research since the discovery of X-rays in 1895. Research in physical sciences using X-rays was started in India by C V Raman at the IACS and made significant contribution in colonial India with limited resources available at that time. By virtue of its scientific activity India made a respectable position in X-ray research globally. IACS acted as the nucleus of X-ray research in India from where it proliferated to different laboratories such as the Benares Hindu University, Allahabad University, Madras University, and the Indian Institute of Sciences, Bangalore.