Prevalence and Risk of Anisakid Larvae in Fresh Fish Frequently Consumed in Spain : An Overview

Anisakidosis is a fish-borne zoonosis caused by parasitic nematodes of the family Anisakidae, of which the species belonging to Anisakis simplex complex are the most representative. It is considered an emerging disease in Spain. The objective of this study is to analyse the presence of larvae in fish frequently consumed in Spanish supermarkets, inferring the risk of infection. In total 1,786 specimens of 9 different fish species, from two geographical origins (Atlantic and Mediterranean), acquired fresh and not eviscerated were examined for anisakid nematodes. Analysis showed that 33.7% of the samples were parasitized by Anisakis larvae. The horse mackerel (Trachurus trachurus) presented the highest total prevalence (66.0%), followed by the silver hake (Merluccius bilinearis) (59.5%), the mackerel (Scomber scombrus) (58.4%), the blue whiting (Micromesistius poutassou) (53.9%) and the European hake (Merlucius merlucius) (45.0%). In general, the prevalence was higher in Atlantic than in Mediterranean fish. In all the species analysed, a higher presence of the parasite was detected in the viscera than in the flesh, although in the most parasitized species a noteworthy prevalence and abundance was observed in the flesh. In conclusion, risk factors, like fish species and origin, should be considered by consumers, in addition of following the recommendations established by Commission Regulation (EU) No1276/2011 and the Spanish Royal Decree 1420/2006.


Introduction
Anisakidosis is a fish-borne zoonosis caused by the ingestion of larval parasitic nematodes of the family Anisakidae, of which species from the Anisakis simplex complex are the most frequent.Humans, who are not suitable hosts for these parasites, acquire the infection accidentally by eating mainly raw, salted marinated or undercooked fish or cephalopods [1].Apart from being the causal agent of a number of gastric, intestinal or ectopic form of pathology [2], Anisakis species are considered to be the only parasites in fishery products that are implicated in allergic reactions [3].Once sensitization has occurred, response to parasite allergens can be acute and generate severe allergic symptoms [4].On account of above mentioned anisakidosis is considered an emerging public health risk for consumers [5,6].
Anisakis spp.larvae can be found parasitizing a wide range of marine teleost species inhabiting the Atlantic and the Mediterranean, as well as the Pacific to the Antarctic area, affecting the fish product Fishes 2019, 4, 13 2 of 16 quality [7].In spite of the large number of studies focusing on the molecular and genetic identification of the parasite [8] and the potential differences in pathogenicity between sibling species [9], there is a scarce information concerning the spatial distribution of anisakid larvae in some economically important fish species [10], which makes it very difficult to ascertain the actual epidemiological status of the parasite and the repercussions on consumers.
The main source of infection are marine fish species as the main ingredient of raw dishes such as sushi, ceviche, sashimi and other similar foods [1].Between 2000 and 2017 a total of 236 not imported cases was reported in the European Union.Spain, in particular, has the highest reported incidence in Europe and the second worldwide [11], with marinated anchovies in vinegar, a typical and very popular Spanish dish, being the main food vehicle of infection [6].
In order to prevent infection, the most effective measure is, according to the recommendations of the Commission Regulation (EU) No1276/2011 [12] and the Spanish Royal Decree 1420/2006 [13], avoiding the consumption of raw or undercooked fish or applying some treatments to inactivate the larvae.These treatments consist of: (1) cooking fish at an internal temperature of 60 • C for at least 1 min.or at 65 • for at least 30 s; (2) freezing at −20 • C or below for at least 24 h, although other authors recommend freezing at −35 • C for at least 15 h or at −15 • C for at least 96 h, at the core of the fishery products [3].Nevertheless, domestic freezers do not always provide a low homogenous temperature and are, therefore, not able to inactivate larvae.The freezing tolerance of these nematodes constitutes a safety risk when domestic freezers operating at low cooling capacity are used for the inactivation of these larvae, as temperatures are not sufficiently low or homogenous [14].Particularly in the case of anchovies in vinegar, some experiments suggest that the consumption of pieces infected with A. simplex may be a potential hazard when consumed by sensitized individuals, even if the larvae are killed by freezing [15].Consequently, the importance of minimizing the infection risk gains importance, which requires the establishment of preventive measures for consumers at the point of purchasing.
The present study analyses the presence of anisakid larvae in nine fish species sold at nationwide supermarkets in the metropolitan area of València, Spain.All these species are frequently consumed in Spain; some of them as part of typical dishes based upon marinated or raw fish as the main ingredient.The specific aims of this work are to assess the epidemiological status of anisakid larvae in fresh fish purchased in nationwide supermarkets, in order to determinate the potential risk of human anisakiosis through the consumption of these species.In addition, to study the influence of extrinsic (origin and season of capture) and intrinsic (weight and length of the fish) factors on the prevalence and abundance of the larvae.The results will shed light on the most important species that may be a source of infection, as well as the factors that affect the presence of the parasite.This knowledge will make it possible to propose measures consumers can apply in order to minimize the risk of diseases.

Results
Considering all the samples as a whole, a total of 663 specimens (40.33%) were parasitized by L 3 larvae of nematodes.After morphological identification, these larvae were classified as Anisakis type I (Anisakidae) and Hysterothylacium spp.(Raphidascaridae), detected in 601 (33.65%) and 207 (11.59%) specimens, respectively.In addition, very few samples of silver hake were parasitized by Contracaecum spp.(Anisakidae).
The results obtained from the nine fish species are given below, while details on prevalence, mean abundance and parasitation range for each fish species related to the variables analysed are presented in Tables 1-4.The comparative results of prevalence and mean abundance in the fish flesh are shown in Figures 1 and 2, respectively.
The mean abundance for the total blue whiting sample was 4.51 helminths per host, being higher for Anisakis type I (3.87) than in Hysterothylacium spp.(0.64).Focusing on Anisakis type I larvae, a larger number of larvae per host was found in the Atlantic fish in respect to the Mediterranean (U = 3375; P < 0.0001; df = 1).A higher abundance was also found in the viscera (3.15) than in the flesh (0.72) (Z = −7.646;P < 0.0001; df = 1).In respect to the season, results were very similar in autumn (4.35) and spring (3.43), without significant difference.
Prevalence was influenced by three factors, that is, fish length, origin and season, when the total sample was considered (χ 2 = 133.103;P = 0.0001; df = 3).Moreover, the number of days that passed between fishing and the analysis influenced the presence of larvae in the flesh (χ 2 = 5.119; P = 0.24; df = 8).The Spearman test showed that weight (Rho = 0.251; P = 0.0001; df = 1) and length (Rho = 0.382; P = 0.0001; df = 1) influenced abundance when all the samples were analysed but not when only Atlantic fish were considered, due to the high prevalence found in both, large and small specimens.
Fish from the Atlantic was more parasitized than Mediterranean fish (χ 2 = 119.78;P < 0.0001; df = 1).Larvae were found more frequently in the viscera (35.00%) than in the flesh (29.50%), although this difference was not significant.Concerning the season of capture, a significant difference was detected (χ 2 = 26.547;P < 0.0001; df = 1), with a higher parasite presence in spring (54.40) than in autumn (7.50).
Considering all the specimens, a mean abundance of 10.22 was found, although the number of larvae per host was higher in the case of Anisakis type I (10.20) than in Hysterothylacium spp.(0.01).The Atlantic sample showed a higher mean abundance (20.34) than the Mediterranean (0.05) (U = 940; P < 0.0001; df = 1).The mean abundance in the viscera (6.50) was higher than in the flesh (3.70), although this difference was not significant.The number of helminths per host also depended on the season (U = 1620; P < 0.0001; df = 1), with a higher mean abundance in spring (12.81) than in autumn (0.36).
Binary logistic regression (BLR) analyses showed that the origin and the length of the fish influenced the prevalence when the entire sample is considered (χ 2 = 157.453;P = 0.0001; df = 2).In addition, the number of days that passed between fishing and the analysis influenced the presence of larvae in the flesh (χ 2 = 16.381;P < 0.0001; df = 1), also when the entire sample is considered.Fish size also influenced on the mean abundance, since the Spearman test showed that both weight (Rho = 0.280; P = 0.0001; df = 1) and length (Rho = 0.319; P = 0.0001; df = 1) influence abundance when the entire sample was analysed, suggesting that larger specimens had a higher number of larvae.

Silver Hake (Merluccius bilinearis)
All the silver hake specimens included in this study were caught in the North-West Atlantic Ocean.Consequently, the origin was not considered as a variable of study in this case.In total 69 specimens (59.48%) were found parasitized by Anisakis type I larvae.No larvae belonging to the genus Hysterothylacium spp.were identified in this fish species.However, 4 specimens were parasitized by larvae belonging to the genus Contracaecum (3.44%).
BLR analyses showed that prevalence was not influenced by any factor.Considering only the parasitized fish, no influence of the number of days that passed between fishing and the analysis was detected.Moreover, length (Rho = 0.259; P = 0.005; df = 1) and weight (Rho = 0.258; P = 0.005; df = 1) also had a positive influence on abundance.

Red Mullet (Mullus barbatus)
The origin was not taken into account as a variable of this study, as the specimens available originated only from the Mediterranean Sea.A total of 43 specimens (27.74%) were parasitized by helminth larvae.In this case both prevalence and abundance of Hysterothylacium spp.(25.16%; 0.58) were higher than those of Anisakis type I (3.23%; 0.05).
No Anisakis larvae were found in the flesh.Anisakis type I prevalence and abundance were lower in spring (1.20%; 0.01) than in autumn (5.30%; 0.08), although this difference was not significant.
Due to the low number of parasitized hosts, it was not possible to carry out a detailed helminthological study as well as an analysis of the influence of extrinsic and intrinsic factors on the infection by Anisakis type I.
Significant differences were found concerning fish origin (χ 2 = 47.347;P < 0.0001; df = 1), showing a higher prevalence in the fish originating from the Atlantic Ocean (76.43%) than in the fish from the Mediterranean Sea (30.77%).The statistical analysis showed a higher percentage of parasitation in the viscera (55.85%) than in the flesh (25.98%) (χ 2 = 42.630;P < 0.0001; df = 1).In contrast, no statistically significant differences were observed between season of capture, with a prevalence of 62.35% in autumn and a prevalence of 56.16% in spring.
A mean abundance of 5.68 Anisakis type I larvae per host was observed, in contrast to Hysterothylacium spp. that had the mean abundance of 0.10 helminths/host only.The mean abundance of Anisakis type I larvae differed in respect to the origin, being higher in the Atlantic (7.29) in comparison to the Mediterranean (3.20) (U = 3647; P < 0.0001; df = 1).Just as in the case of prevalence, a higher mean abundance was detected in the viscera (4.65) than in the flesh (1.03) (Z = 8318; P < 0.0001; df = 1).Significant differences were not observed according to the season of capture, showing a mean abundance of 4.47 in spring and 7.75 in autumn.

Chub Mackerel (Scomber japonicus)
The only helminth larvae found in this fish species was identified as Anisakis type I.A total of 19 specimens (17.59%) was parasitized, with a mean abundance of 1.11.
Fish from the Atlantic Ocean was more parasitized (19.18%) than the Mediterranean fish (14.29%), although this difference was not significant.A higher presence of larvae was found in the viscera (16.70%) than in the flesh (2.80%) (χ 2 = 10.338;P = 0.0009; df = 1).Concerning the season of capture, in spring the prevalence was higher (22.00%) than in autumn (19.20%), although the difference was not significant.
Just as in the case of prevalence, the mean abundance was higher in the Atlantic Ocean (1.84) than in the Mediterranean Sea (0.17), although the difference was not significant.The mean abundance was higher in the viscera (1.07) than in the flesh (0.04) but without statistical significance.However, the mean abundance was greater in fish captured in autumn (1.84) than in fish captured in spring (0.26) but without statistical differences.BLR analysis showed that the prevalence of the entire sample was not influenced by any factor.Likewise, the number of days that passed between fishing and the analysis did not have any influence on the prevalence.Moreover, the statistical analysis also showed that none of the extrinsic and intrinsic factors considered influenced the mean abundance.
All specimens originating from the Atlantic were found parasitized by Anisakis type I larvae and only 16 (32.00%) of those from the Mediterranean (χ 2 = 48.529;P < 0.0001; df = 1).A higher presence of the parasite was found in the viscera (60.00%) compared to the flesh (28.00%), although this difference was not statistically significant.
BLR analysis showed that none of the factors considered, influenced the prevalence.Concerning the presence of parasites in the flesh and the number of days that passed between the catch and the analysis of the specimens, no relationship was found.
The mean abundance of the total batch was 2.94, with a very low number of Anisakis type I (0.20) and a higher value of Hysterothylacium spp.(2.75).The total larval burden was higher in the Atlantic fish (0.34) than in that from the Mediterranean (0.05) (U = 8950; P = 0.002; df = 1).The total mean abundance was higher in the viscera (0.17) than in the flesh (<0.01) (Z = −6379; P < 0.0001; df = 1), in spite of the low number of larvae found.No differences were detected concerning the seasons.

Discussion
The present study provides epidemiological data on the presence of Anisakis type I and other anisakid L 3 larvae in fish frequently consumed in Spain, in order to assess the risk of anisakidosis through fresh fish purchased in the Spanish supermarkets.Moreover, the presence of larvae of other nematode species (Hysterothylacium spp.), resembling anisakids to a non-expert, parasitizing these fish species is reported, posing additional issues associated to the quality of the product.It is important to highlight, as the supermarkets included in the study all belong to nationwide chains that sell fish originating from almost the same fishery areas, that the results obtained in this study can be extrapolated to all Spanish supermarkets.
Results revealed that all the fish species were parasitized by Anisakis type I larvae with a global prevalence of 33.65%.Anisakis species have been reported in a wide range of marine pelagic fish and cephalopods [16][17][18], most of them encompassing an important part of the human diet.As fish is considered a staple of the healthy diet, particularly in Spain [19], the results reported herein pinpoint the fish species with a high risk of infection.
Nevertheless, there are considerable differences in the infection risk depending on the fish species analysed: i) "high-risk" species, with a high rate of infection; ii) "intermediate or low-risk" species, with a low rate of infection or with a scarce presence of larvae in the edible parts.
Five of the studied species can be classified as "high risk" species: the blue whiting, the European hake, the silver hake, the mackerel and the horse mackerel.Considering the prevalence and abundance registered, all of them could represent a high risk of infection when consumed raw, marinated or poorly cooked, if not being adequately frozen beforehand.In general, this observation is in accordance with other results previously reported [20][21][22][23][24][25][26][27][28][29][30].
Results concerning the blue whiting, assessed previously in depth by Madrid et al. [31], are in agreement with other studies, confirming this as one of the most parasitized fish species [20][21][22].The blue whiting is one of the main fin fish species consumed in Spain [32], due to its low cost and its easy mode of preparation.The same goes for the European hake, another of the fin fish analysed in which a significant prevalence was detected, also reported by other authors [23][24][25].The silver hake, of which there are scarcely similar studies in the literature [23], had a high prevalence, although the abundance was lower in comparison to the other "high risk" species.In this fish species, the finding of Contracaeum spp.L 3 stands out, since this was the only species in which this anisakid was found.Nevertheless, in spite of the reports of anisakidosis cases caused by Contracaecum spp.larvae [33], their potential pathogenicity in humans is under discussion [34].
The mackerel and the horse mackerel, the two "high risk" pelagic fish species, consumed frequently marinated or pickled in Spain, were two of the most infected species.Other authors have also reported high values of parasitation both in the mackerel [10,35] and the horse mackerel [26][27][28], suggesting that these species may involve a serious source of infection if safety measures are not correctly applied.A more extensive study concerning anisakid larvae in the mackerel was carried out by Madrid et al. [29].
In respect to the number of larvae per host, a total mean abundance of 3.99 was observed, with large differences depending on the fish species.The horse mackerel was the species with the highest mean abundance in the present study, being well above the rest.The European hake, the mackerel and the blue whiting also presented a high mean abundance.The helminth burden was in accordance with previous studies evidencing a high mean abundance in these "high risk" fish species.However, in the case of the horse mackerel, the mean abundance was greater than in some previous reports [27,28,30].
The other fish species studied (the red mullet, the chub mackerel, the sardine and the anchovy) can a priori be considered "intermediate or low-risk" species, due to the lower values of prevalence and abundance in comparison to the others.In the case of the red mullet, there are only few works focusing on the presence of anisakids, although all of them reported a low prevalence of Anisakis spp.[36,37].A more extensive study on helminth parasites of the red mullet was carried out by Debenedetti et al. [38].
The chub mackerel is a pelagic fish species frequently consumed in Japan, where a very high prevalence was reported [39].In Spain, this fish species is less frequent, often being confused with the mackerel due to their similar appearance.In the present study, a relatively high prevalence was found, lower than the prevalence recorded by other authors for the same fishing areas [40], although the lower presence of larvae in the flesh makes it possible to consider this species an "intermediate-risk" fish.Consequently, it is important for the consumer to differentiate between the mackerel and the chub mackerel, given their different risk level for anisakidosis, high for the former and intermediate for the latter.
The sardine is generally considered a fish with a low risk of anisakiosis, since many authors have either reported the absence of Anisakis spp.larvae [35,41] or a very low prevalence [42,43].In the present study, a low prevalence and abundance was found, although the values are higher in comparison to the previous studies.However, some cases of anisakiosis due to the consumption of raw sardines have been reported in Spain [44,45].
The anchovy is one of the most popular fish consumed in Spain, mainly marinated in vinegar, usually for 24 h, known as "boquerones in vinegar."Therefore, it is also considered the main source of anisakidosis in this country through the consumption of this home-made dish [6,45].In the present study, a prevalence of 14.47% was observed, which is apparently low but may still involve a serious risk of infection.Other authors reported a higher prevalence and abundance in this fish from central areas of the Mediterranean, such as the NE part of the Mediterranean Sea and the Atlantic waters, showing percentages of parasitation and abundances in general higher than those reported herein [10,46,47].However, some studies reported the absence of anisakids or a prevalence below 10%, suggesting that anchovies are rarely infected [35,[48][49][50].Nevertheless, the prevalence of larvae in the flesh is relatively high in comparison to the other "low-risk" fish.Therefore, taking into account that this fish species is usually consumed raw and marinated in vinegar, the potential presence of larvae in the flesh has to be considered.In addition to this, some experiments suggest that the consumption of anchovies in vinegar parasitized with Anisakis sp.larvae may pose a risk to sensitized individuals, even if the larvae are killed by freezing [15,50].Consequently, the anchovy should be included in the "high-risk" species group.
Given the origin of the fish species analysed, specimens originating from the Atlantic, when compared to the Mediterranean, were more parasitized, usually with statistically significant differences.In total 52.90% of the fish captured in the Atlantic were parasitized by Anisakis type I larvae, whereas the percentage was only 11.92 from the Mediterranean.This finding is in accordance with previous studies carry out in various teleost species [11,21,26,42,[51][52][53]. Therefore, the origin of the fish should be taken into consideration by consumers in order to minimize the infection risk.
The host size (length and weight) was an influent factor for parasitation in almost all the fish species analysed, suggesting that larger specimens may involve a higher risk of infection.Only in the case of the horse mackerel, this factor was not significant, although such lack of significance is mainly due to the high prevalence observed and the differences in size depending on the origin.This made it impossible to analyse the entire sample as a whole.There are numerous works that reported the influence of this factor on some fish species [46,48,51,[54][55][56][57].The host size is directly related to the age of the host and therefore, the high level of parasitation in older specimens may be due to the accumulation of larvae along time and the higher probabilities of infection, leading to a higher level of abundance and prevalence [57].
The prevalence observed in spring was, in general, higher than in autumn, although this factor does not seem to play an important role, since the European hake and the anchovy were the only species in which seasonal differences were significant.These differences are probably related to the seasonal patterns of migration of these fish species, as well as to the effect of the season on the presence of euphausiid crustaceans in the environment, which encompass an important item in the food chain of these hosts [23].Several studies have reported seasonal differences of parasitation [27,48,57,58].It is noteworthy that in some of the species analysed the prevalence was higher in one season, while the abundance was higher in the other one, as other authors also reported [59], probably due to a regulation mechanism of the parasite in order to close its life cycle.
In all the species analysed, a higher presence of the parasite was detected in the viscera than in the flesh, although in the most parasitized species (the blue whiting, the European hake, the silver hake, the mackerel and the horse mackerel), the prevalence in the flesh was remarkable, within a range of 25.98%-29.50%.The mean abundance also was noteworthy, particularly in the case of the mackerel and the horse mackerel, with a range of 1-3.70 larvae per host.Considering the life cycle of anisakids [60], the preferred parasitation site of larvae is the viscera and other body cavities, although it is known that they are able to migrate and to spiralise in the flesh of the host [7].Considering that the flesh is the main edible part, the presence of larvae in the flesh involves a higher risk for consumers.Nevertheless, the presence of the larvae in the viscera should not be ignored, since the flesh can easily be contaminated when the specimen is eviscerated and handled.
Furthermore, a significant effect of the fish storage on the presence of larvae in the flesh was found in the blue whiting, the European hake and the mackerel, being suggestive of the migratory capability of these larvae evaluated through the number of days that passed after the catch.Other authors have also reported that Anisakis spp.larvae are able to migrate from the visceral organs to the flesh after the death of the host, suggesting that this movement may be facilitated by the cold storage or processing of uneviscerated fish [53].In contrast, other experiments suggest that keeping the fish at low temperature could reduce larval migration [61].Particularly, in the present study, the species in which this correlation was significant were those with the highest prevalence and abundance values.The accumulation of larvae in the viscera could promote their migration to the flesh.In the case of the horse mackerel this effect was not found, which can be explained by the large number of larvae detected in both sites, making it impossible to establish the correlation through the statistical analysis.Therefore, the capability of migration in this fish species should not be ruled out.
The finding of other nematode species cannot be overlooked, not only because of their potential capacity of causing pathology in humans but also because of their deleterious effect at a commercial level.The high percentage of Hysterotylacium spp.larvae observed in some fish species must be seriously considered.Their high prevalence and abundance, particularly in the horse mackerel, are higher than in previous studies [26,54].In contrast, other fish species, also highly parasitized by Anisakis type I, showed a lower prevalence (i.e., the blue whiting, the mackerel and the European hake) or were not parasitized at all (i.e., the silver hake and the chub mackerel) by Hysterotylacium spp.larvae.Moreover, in the fish species in which a low or very low prevalence and abundance of Anisakis type I larvae were found (i.e. the red mullet, the sardine and the anchovy), a higher presence of Hysterotylacium spp.larvae was detected, with a prevalence range of 18-25%.These data are in accordance with previous studies, which in general also recorded a high presence of this parasite in particular fish species [37,41,49].It is true that this nematode is considered not potentially harmful for human health, with only isolated cases reported in the literature related to its presence in the human gut [62,63].However, they do seem to produce a significant pressure on the host visceral organs [64], causing a pathogenic effect in live fish as well as an economic loss given the devaluation of the commercial product.Consequently, consumers must be warned about the presence and the potential effect of the parasite, avoiding the possible confusion with Anisakis spp.larvae.

Samples
A total of 1.786 specimens belonging to nine fish species was selected for this survey, all of them species frequently consumed in Spain.The species included in the survey were the blue whiting (Micromesistius poutassou), the European hake (Merluccius merluccius), the silver hake (M.bilinearis), the red mullet (Mullus barbatus), the mackerel (Scomber scombrus), the chub mackerel (S. japonicus), the horse mackerel (Trachurus trachurus), the sardine (Sardina pilchardus) and the anchovy (Engraulis encrasicolus).These species and the number of specimens are shown in Table 5.All the specimens were purchased in branches of four nationwide Spanish supermarket chains located in València and its metropolitan area.The fish analysed were obtained in the period from 2006 to 2015, as part of a project on the potential risk of human anisakidosis through the consumption of fresh fish in Spain, conducted by the Parasites and Health Research Group of the Department of Pharmacy and Pharmaceutical Technology and Parasitology, at University of València (Spain).Some of these results have been previously published [29,31,38].
The area of origin, Atlantic, Food and Agriculture Organization of the United Nations [FAO] zones 21 and 27 (subareas IV, VIII and IX) or Mediterranean Sea, FAO zone 37.1, division 1.1, as well as the date of capture were taken from the fish label at the time of purchase.The samples were chosen at random, fresh and non-eviscerated and in the laboratory were kept in a fridge at 4 • C until dissection.Each sample was weighed and the total length was measured.Finally, specimens were also grouped according to season of capture, distinguishing two periods: spring and autumn.This variable was not studied in the case of the horse mackerel, as the sample was obtained in spring only.The number of specimens analysed with respect to the origin and the season of capture is shown in Table 5.Table 6 presents data of the weight and length range, as well as the number of days that passed between capture and analysis.

Fish Inspection
Each specimen was analysed for the presence of anisakid larvae, after conventional dissection.The viscera and flesh of each specimen were placed in two different Petri dishes and examined separately.The viscera were dissected under a stereoscopic microscope and the flesh, after a previous visual inspection, underwent artificial enzymatic digestion [65].Resulting product from the digestion was also examined under a stereoscopic microscope.

Morphological Identification
All the anisakids found were identified according to the morphological characters described in the literature [66][67][68].The main characteristics considered for these classifications were the position of the excretory pore, the arrangement and separation of the digestive tract into oesophagus, ventricle and the presence/absence of structures such as intestinal caeca and oesophageal appendix, as well as the shape of the tail.

Statistical Analysis
The number of infected hosts, range of parasitation, prevalence and mean abundance were analysed according to Bush et al. [69] for the total of anisakids found, as well as for each of the genera identified.Moreover, these parameters were also calculated for the site of parasitation (viscera and flesh), the season and origin of capture.In the case of the mean abundance, the confidence interval (95% CI), using the bootstrap method, was also calculated [70,71].
The study was carried out solely for the isolation and identification of Anisakis spp.larvae, as it is the only species that survives artificial digestion and consequently poses a risk of human infection.The analysis comprised the comparison of prevalence (χ 2 test) and abundances (Mann-Whitney (U) test) and sites of parasitation (Wilcoxon (Z) test).The potential risk of human anisakidosis was assessed analysing the influence of intrinsic (weight and length) and extrinsic (origin and season) factors on the prevalence and abundance of Anisakis spp.larvae, by binary logistic regression (BLR) for prevalence and the Spearman test (Rho) for abundance.Furthermore, the potential influence of the number of days after capture (which corresponds to the theoretical day of consumption, 1-8 days) on the presence of larvae in the flesh was also analysed by BLR, considering parasitized hosts only.Statistical significance was established at P<0.05.Statistical analyses were carried out using StatView 5.0 (SAS Institute Inc., Cary, North Carolina, USA) and SPSS 19.0 for Windows (SPSS Inc., Chicago, Illinois, USA) software packages.

Conclusions
The present study presents an overview on the prevalence and the risk of anisakid larvae in fresh fish frequently consumed in Spain.As the fish was purchased in selected supermarket chains present in the entire Spanish territory, the results obtained in the study area can be extrapolated to the entire country.Global prevalence and abundance of parasitation by Anisakis larvae were found to be high, with the blue whiting, the European hake, the silver hake, the mackerel, the horse mackerel and the anchovy presenting a serious risk of Anisakis spp.infection.
The fish originating from the Atlantic and specimens with a larger size present the highest risk, not only because these groups showed the highest values of infection but also prevalence and abundance higher in the fish flesh.In addition to this, the capacity of larval migration after prolonged storage in some of these "high-risk" species was observed, suggesting that immediate evisceration after capture recommendable as an effective measure to prevent parasite presence.
To minimize the risk of anisakiosis, consumers have to follow the preventive measures recommended by Commission Regulation (EU) No1276/2011 [12] and by Spanish Royal Decree 1420/2006 [13]: freezing fish at -20 • C, for at least 24 h or cooking the fish until reaching a core temperature of 60-70ºC for 5 to 10 min..The infection risk can be minimized if traceability data

Figure 1 .
Figure 1.Classification of the fish species based on the prevalence (%) of Anisakis type I larvae.

Figure 1 .
Figure 1.Classification of the fish species based on the prevalence (%) of Anisakis type I larvae.

Figure 1 .
Figure 1.Classification of the fish species based on the prevalence (%) of Anisakis type I larvae.

Figure 2 .
Figure 2. Classification of the fish species based on the mean abundance (helminths/host) of Anisakis type I larvae observed in the fish flesh.

Table 1 .
Prevalence (%), mean abundance and 95% confidence intervals, intensity range (minimum-maximum) and number of Anisakis type I larvae in the total sample.

Table 2 .
Prevalence (%) and mean abundance and 95% confidence intervals of Anisakis type I larvae in the fish species analysed with respect to their geographic origin.

Table 3 .
Prevalence (%) and mean abundance and 95% confidence intervals of Anisakis type I larvae in the fish species analysed with respect to the microhabitat of parasitation.

Table 4 .
Prevalence (%) and mean abundance and 95% confidence intervals of Anisakis type I larvae in the fish species analysed with respect to season of capture.

Table 5 .
Origin and season of capture of the sample.

Table 6 .
Weight, length and days passed between capture and analysis of the sample.