An Overview of Fruit Allergens: Structural, Functional, Phylogenetical, and Clinical Aspects

: Most of the allergenic proteins from fruits identiﬁed so far belong to different families of pathogenesis-related (PR) proteins. These PR proteins have been classiﬁed in different families of structurally and functionally unrelated proteins, but the majority of all fruit allergens belong to three groups, in particular PR-5 thaumatin-like proteins (TLP), PR-10 Bet v 1-like proteins, and PR-14 non-speciﬁc lipid transfer proteins (nsTLP). Some allergenic proteins from fruits can also be found among PR-protein families of PR-2 β 1,3-glucanase proteins, PR-3 chitinases I, II, IV–VII, and PR-8 chitinases III. In addition, other important fruit allergens occur in protein families unrelated to the PR-protein families, such as the proﬁlins and the newly emerging group of gibberellin-regulated proteins (GBRP). Finally, proteins that belong to seed storage proteins from higher plants, including 2S albumins, 7S globulins (vicilin), and 11S globulins (legumin), must be retained as possible potential fruit allergens resulting from the unintended consumption of the seeds. Here, we present an overview of the structural organization, functional properties, and phylogenetical relationships among these different groups of fruit allergens, supporting the occurrence of cross-reactivity and cross-allergenicity often described between fruit allergens, and the corresponding allergens from vegetables and pollens.


Introduction
Allergies to edible fruits have grown dramatically during the last years, due to increased fruit consumption and the introduction of many exotic fruits into our eating habits.Edible fruits, especially fleshy fruits, contain allergenic proteins that are responsible for various allergic manifestations, ranging from a simple oral syndrome (OAS) to a more severe anaphylactic shock.In this respect, fruits from the Rosaceae family, and kiwi fruits from the Actinidiaceae family, have become a worrying source of food allergies largely distributed in many countries.
Botanically, fruits are derived from the transformation and development of the ovary from flower parts after pollination.Depending on the fruits, the transport of the pollen to the pistil is either self-pollinated, or carried out by insects (entomophilous plants) or wind (anemophilous plants).In fleshy fruits from the Rosaceae family, the edible part of the fruit consists of the mesocarp and the exocarp, which develop from the ripened ovary or carpels and the floral envelopes, respectively.The exocarp is limited by the skin or epicarp.The seeds, derived from the pollinated ovules, are located in the endocarp (Figure 1).In many fruits from the Rosaceae family, such as peach (Prunus persica), apricot (Prunus armeniaca), or plum (Prunus dulcis), the mesocarp and endocarp are sclerified and form a kernel.A similar organization occur in other fruits from the Solanaceae (tomato), Cucurbitaceae (pumpkin), Musaceae (banana) or Lythraceae (pomegranate) families.Besides the commonly consumed fleshy fruits, e.g., fruits from t a large diversity of edible fruits has been described, including the cary (maize, wheat, rice) which are, however, essentially made up of the fore considered more like seeds, or false fruits like strawberries (Frag from the development of the calyx accrescent from the flowers covere of achenes containing tiny seeds.Accordingly, defining edible fruits because, in common language, some genuine fruits (tomato, bell pep often considered as vegetables and, vice versa, some so-called fruits (r ily correspond to genuine vegetables [1]. Most of the allergenic proteins from fruits identified so far belong of PR-protein [2].PR proteins correspond to a disparate set of prote their structures and properties, and are all involved in various proces against phytopathogens and, more generally, in all the stress situati to face.PR proteins have been classified in seventeen distinct familie functionally unrelated proteins [3], but the majority of all fruit allerg families, in particular PR-5 thaumatin-like proteins (TLP), PR-10 Bet v PR-14 non-specific lipid transfer proteins (nsTLP) (Figure 2).Other such as PR-2 β1,3-glucanase proteins, PR-3 chitinases I, II, IV-VII, and also contain some allergenic proteins from fruits.However, other imp are not related to the PR-protein families, such as the profilins and group of gibberellin-regulated proteins (GBR-protein).Although no the small knottin-folded defensins occur as food allergens susceptib with their well-identified pollen allergen counterparts.In addition, cluding Cuc m 3 of the PR-1 family, bromelain (Ana c 2) and kiwelin single fruit or a restricted number of fruits.Finally, proteins that belon of seed storage proteins from higher plants, 2S albumins, 7S globuli Besides the commonly consumed fleshy fruits, e.g., fruits from the Rosaceae family, a large diversity of edible fruits has been described, including the caryopses from Poaceae (maize, wheat, rice) which are, however, essentially made up of the seeds and are therefore considered more like seeds, or false fruits like strawberries (Fragaria vesca), resulting from the development of the calyx accrescent from the flowers covered with an aggregate of achenes containing tiny seeds.Accordingly, defining edible fruits is not an easy task because, in common language, some genuine fruits (tomato, bell pepper, cucumber) are often considered as vegetables and, vice versa, some so-called fruits (rhubarb stalks) readily correspond to genuine vegetables [1].
Most of the allergenic proteins from fruits identified so far belong to different families of PR-protein [2].PR proteins correspond to a disparate set of proteins, which differ in their structures and properties, and are all involved in various processes of plant defense against phytopathogens and, more generally, in all the stress situations that plants have to face.PR proteins have been classified in seventeen distinct families of structurally and functionally unrelated proteins [3], but the majority of all fruit allergens belong to three families, in particular PR-5 thaumatin-like proteins (TLP), PR-10 Bet v 1-like proteins, and PR-14 non-specific lipid transfer proteins (nsTLP) (Figure 2).Other PR-protein families, such as PR-2 β1,3-glucanase proteins, PR-3 chitinases I, II, IV-VII, and PR-8 chitinases III, also contain some allergenic proteins from fruits.However, other important fruit allergens are not related to the PR-protein families, such as the profilins and the newly emerging group of gibberellin-regulated proteins (GBR-protein).Although not restricted to fruits, the small knottin-folded defensins occur as food allergens susceptible to cross-reaction with their well-identified pollen allergen counterparts.In addition, a few allergens, including Cuc m 3 of the PR-1 family, bromelain (Ana c 2) and kiwelin (Act c 5), belong to a single fruit or a restricted number of fruits.Finally, proteins that belong to the three classes of seed storage proteins from higher plants, 2S albumins, 7S globulins (vicilin), and 11S globulins (legumin) [4], should also be retained as possible potential fruit allergens resulting from the unintended consumption of the seeds of kernels [5].Many of these fruit allergens represent widely distributed pan-allergens, which are responsible for IgE-binding cross-reactivity often associated with some cross-allergenicity.Here we present a review on the molecular structures and properties of the main categories of fruit allergen families, with some insights into the clinical aspects of the corresponding food allergies.

Repertoire of the Main Fruit Allergen Families 2.1. PR-5 Thaumatin-like Proteins (TLP)
The group of PR-5 thaumatin-like proteins (TLP) is characterized by an extreme homogeneity of both the molecular structure and the endo-β1,4-glucanase properties associated with their structural fold.All TLPs possess a structure similar to that found in thaumatin, the sweet-tasting protein isolated from the arils of Thaumatococcus daniellii [6], made of three covalently associated domains: a central domain I with a β-sandwich structure, flanked on both sides by a predominantly α-helical domain II, and a small domain III which consists of a short β-hairpin (Figure 3A) [7].Up to 16 extremely conserved cysteine residues form 8 disulfide bridges that confer to this tightly structured fold a good resistance to digestive proteases and heat denaturation, both properties susceptible to enhance the allergenicity of protein allergens [8].A highly electronegative cleft with an endo-β1,4-glucanase activity, occurs between domains I and II (Figure 3B,C).
III which consists of a short β-hairpin (Figure 3A) [7].Up to 16 extremely con teine residues form 8 disulfide bridges that confer to this tightly structured resistance to digestive proteases and heat denaturation, both properties susce hance the allergenicity of protein allergens [8].A highly electronegative cleft w β1,4-glucanase activity, occurs between domains I and II (Figure 3B,C).The allergenicity of fruit TLPs has been scarcely investigated and, thus insights are available on the IgE-binding epitopic regions of fruit TLPs.An proach allowed the prediction of four IgE-binding epitopic areas correspon amino acid sequence stretches 36-48, 51-63, 58-70, and 73-85 on the surface dilla (Manilkara zapota) fruit, that are essentially located around the electron on the front face of the allergen (Figure 4A) [9].Previously, some overlap h tected between the predicted IgE-binding epitopes from Mus a 4, the banan minata) TLP, and the IgE-binding epitopes identified in the mountain cedar Jun a 3 [7].These B-cell epitopes occur in the α-helical domain II of the TLP.M three IgE-binding epitopes from Cup s 3, the green cypress (Cupressus sempe allergen, and TLPs from other phylogenetically related Cupressaceae species, teracted with all the tested allergic patient's sera, using the Pepscan technique [10].The presence of common epitopes offers a molecular basis for the cross-re crossed allergenicity observed between fruit and pollen TLP allergens [11].The allergenicity of fruit TLPs has been scarcely investigated and, thus, only a few insights are available on the IgE-binding epitopic regions of fruit TLPs.An in silico approach allowed the prediction of four IgE-binding epitopic areas corresponding to the amino acid sequence stretches 36-48, 51-63, 58-70, and 73-85 on the surface of the sapodilla (Manilkara zapota) fruit, that are essentially located around the electronegative cleft on the front face of the allergen (Figure 4a) [9].Previously, some overlap had been detected between the predicted IgE-binding epitopes from Mus a 4, the banana (Musa acuminata) TLP, and the IgE-binding epitopes identified in the mountain cedar pollen TLP Jun a 3 [7].These B-cell epitopes occur in the α-helical domain II of the TLP.More recently, three IgE-binding epitopes from Cup s 3, the green cypress (Cupressus sempervirens) TLP allergen, and TLPs from other phylogenetically related Cupressaceae species, strongly interacted with all the tested allergic patient's sera, using the Pepscan technique (Figure 4b) [10].The presence of common epitopes offers a molecular basis for the cross-reactivity and crossed allergenicity observed between fruit and pollen TLP allergens [11].The multiple amino acid sequence alignments of TLP allergens show a high degree of conservation that corresponds to a very conserved structural organization (Figure 5).However, TLPs from the Rosaceae family slightly differ from TLPs of other families by two additional insertions in regions corresponding to domain I (1st insertion) and III (2nd insertion), respectively.
ferently colored.The electronegative catalytic cleft is highlighted with a yellow line.
The multiple amino acid sequence alignments of TLP allergens show a high degree of conservation that corresponds to a very conserved structural organization (Figure 5).However, TLPs from the Rosaceae family slightly differ from TLPs of other families by two additional insertions in regions corresponding to domain I (1st insertion) and III (2nd insertion), respectively.The TLPs of Rosaceae form a very homogeneous group of closely related proteins, whereas TLPs from other families are clustered in a rather distant group within the phylogenetic tree of TLPs built from the amino acid sequence comparison.TLPs from the Cupressaceae pollen form an intermediate cluster equidistant from the other two fruit clusters (Figure 6).Depending on these phylogenetic affinities, the IgE-binding cross-reactivities observed among the fruit TLPs, e.g., between Mal d 2, Pru av 2 and Mus a 4, are so The TLPs of Rosaceae form a very homogeneous group of closely related proteins, whereas TLPs from other families are clustered in a rather distant group within the phylogenetic tree of TLPs built from the amino acid sequence comparison.TLPs from the Cupressaceae pollen form an intermediate cluster equidistant from the other two fruit clusters (Figure 6).Depending on these phylogenetic affinities, the IgE-binding crossreactivities observed among the fruit TLPs, e.g., between Mal d 2, Pru av 2 and Mus a 4, are so frequent than those observed between fruit and pollen TLPs, e.g., between Mal d 2, Pru av 2 and Cup a 3 [12].virginiana Jun v 3, Thuya occidentalis Thu oc TLP) of higher plants.TLP allergens of fruits and pollen are highlighted in red and green, respectively.TLP allergens located in vegetative organs of plants are in bold letters.

PR-10 Bet v 1-like Proteins
Bet v 1, the major pollen allergen of birch (Betula verrucosa), has been identified for a long time as the essential component of the birch pollen allergy [13].Bet v 1 and other closely related PR-10 Bet v 1-like allergens consist of a homogeneous group of polypeptide chains of about 150 amino-acid residues, typically folded as an αβ protein made of a large curved β-sheet associated with a long α-helix (Figure 7A), exhibiting an overall cylindrical shape (Figure 7B,C) [14].These loosely structured proteins exhibit a weak resistance to digestive proteases and heat denaturation, so they are quickly inactivated upon the cooking of PR-10 Bet v 1 like protein-containing foods and food products [15].

PR-10 Bet v 1-like Proteins
Bet v 1, the major pollen allergen of birch (Betula verrucosa), has been identified long time as the essential component of the birch pollen allergy [13].Bet v 1 and o closely related PR-10 Bet v 1-like allergens consist of a homogeneous group of polypep chains of about 150 amino-acid residues, typically folded as an αβ protein made of a l curved β-sheet associated with a long α-helix (Figure 7A), exhibiting an overall cylind shape (Figure 7B,C) [14].These loosely structured proteins exhibit a weak resistanc digestive proteases and heat denaturation, so they are quickly inactivated upon the c ing of PR-10 Bet v 1 like protein-containing foods and food products [15].Structural and mutational analyses of Pru av 1 showed that the amino acid sequence stretch 44LEGDGGPGT52, forming the so-called P-loop of Pru av 1 and other PR10 Bet v 1-like protein allergens, played a key role in the IgE-binding capacity of Pru av 1, together with two other residues N28 and P108, located in other parts of the protein (Figure 8) [16,17].In addition, the residue stretch 142-156 of Bet v 1 was identified as the dominant T-cell epitope of the major birch pollen allergen [18].All these key residues participating in the discontinuous IgE-binding epitopes and T-cell epitopes should be responsible for the cross-reactivity between food and pollen Bet v 1-like proteins [19].
Allergies 2023, 3, FOR PEER REVIEW 1-like protein allergens, played a key role in the IgE-binding capacity of Pru av 1, togeth with two other residues N28 and P108, located in other parts of the protein (Figure [16,17].In addition, the residue stretch 142-156 of Bet v 1 was identified as the domin T-cell epitope of the major birch pollen allergen [18].All these key residues participati in the discontinuous IgE-binding epitopes and T-cell epitopes should be responsible the cross-reactivity between food and pollen Bet v 1-like proteins [19].The multiple amino acid sequence alignment of PR10-Bet v 1-like allergens show high degree of conservation (Figure 9), which results in a very conserved structural ganization for all the members of the PR10 family (Figure 8).The multiple amino acid sequence alignment of PR10-Bet v 1-like allergens show a high degree of conservation (Figure 9), which results in a very conserved structural organization for all the members of the PR10 family (Figure 8).The multiple amino acid sequence alignment of PR10-Bet v 1-like allergens show a high degree of conservation (Figure 9), which results in a very conserved structural or ganization for all the members of the PR10 family (Figure 8).Bet v 1 allergens from the Fagaceae pollen form a homogeneous group of proteins sharing conserved amino acid sequences and closely-related three-dimensional structures (Figure 10) [20].However, Bet v 1-like allergens exhibiting very close amino acid sequences and structural scaffolds also occur in various fruits from the Rosaceae family (Fra a 1, Mal d 1, Pru p 1, Pru ar 1, Pru av 1), seeds (Gly m 4, Jug r 5), and vegetables (Api g 1, Dau c 1), that account for possible cross-reactivities and cross-allergies with these food products.
Bet v 1 allergens from the Fagaceae pollen form a homogeneous group of proteins sharing conserved amino acid sequences and closely-related three-dimensional structures (Figure 10) [20].However, Bet v 1-like allergens exhibiting very close amino acid sequences and structural scaffolds also occur in various fruits from the Rosaceae family (Fra a 1, Mal d 1, Pru p 1, Pru ar 1, Pru av 1), seeds (Gly m 4, Jug r 5), and vegetables (Api g 1, Dau c 1), that account for possible cross-reactivities and cross-allergies with these food products.

PR14 Non-Specific Lipid Transfer Proteins (nsLTP)
Non-specific lipid transfer proteins (nsLTPs) are small polypeptides of about 90 amino acids, which consist of a tightly packed core of four α-helices (α1-α4) extended by a C-terminal tail, which adopt the so-called "saxophone-like" conformation (Figure 11) [21].Four disulfide bridges occurring between 8 conserved cysteine residues contribute to creating a compact structure extremely resistant to digestive proteases and heat denaturation that probably accounts for their high allergenic potential [22,23].In fleshy fruits like

PR14 Non-Specific Lipid Transfer Proteins (nsLTP)
Non-specific lipid transfer proteins (nsLTPs) are small polypeptides of about 90 amino acids, which consist of a tightly packed core of four α-helices (α1-α4) extended by a Cterminal tail, which adopt the so-called "saxophone-like" conformation (Figure 11) [21].Four disulfide bridges occurring between 8 conserved cysteine residues contribute to creating a compact structure extremely resistant to digestive proteases and heat denaturation that probably accounts for their high allergenic potential [22,23].In fleshy fruits like peach, apple or apricot, once synthesized in the pulp cells, nsLTPs migrate into the epidermic cells to accumulate in the fuzzy covering the peach or the outer cuticular cells of the apple [24].As a practical consequence of this surface localization, peeling the skin off the peach and apple allows the removal of most of the allergens contained in these fruits.
Allergies 2023, 3, FOR PEER REVIEW 10 peach, apple or apricot, once synthesized in the pulp cells, nsLTPs migrate into the epidermic cells to accumulate in the fuzzy covering the peach or the outer cuticular cells of the apple [24].As a practical consequence of this surface localization, peeling the skin off the peach and apple allows the removal of most of the allergens contained in these fruits.Three-major IgE-binding epitopic regions have been identified on the surface of the Three-major IgE-binding epitopic regions have been identified on the surface of the Rosaceae nsLTPs, Pru p 3 [25] and Mal d 3 [26], probing synthetic peptides spanning the nsLTP sequences with specific IgE-containing sera from allergic patients.Although few in number, these IgE-binding epitopic regions cover an extended area of the molecular surface, which could explain the enhanced allergenicity of both allergens (Figure 12).In addition, most of these IgE-binding regions are well conserved in nsLTPs from other fruits and other sources, like seeds and pollen [27].Three-major IgE-binding epitopic regions have been identified on Rosaceae nsLTPs, Pru p 3 [25] and Mal d 3 [26], probing synthetic pep nsLTP sequences with specific IgE-containing sera from allergic patien number, these IgE-binding epitopic regions cover an extended area of face, which could explain the enhanced allergenicity of both allergens dition, most of these IgE-binding regions are well conserved in nsLTP and other sources, like seeds and pollen [27].A high degree of conservation of their amino acid sequences char nsLTP allergens, as shown in the amino acid sequence alignment (Figu A high degree of conservation of their amino acid sequences characterizes the nsLTP allergens, as shown in the amino acid sequence alignment (Figure 13).
In this respect, the phylogenetic tree built for nsLTPs of different origins shows some rather close relationships of fruit nsLTPs with other seeds and pollen nsLTPs, irrespective of their phylogenetic relationships (Figure 14).In this regard, nsLTP allergens from the Rosaceae family form a very homogeneous group with a high degree of cross-reactivity among the members, e.g., strong cross-reactivity of Pru p 3 with Mal d 3, Pru av 3, and Pru ar 3, and lower cross-reactivity with nsLTP allergens from other families, e.g., low crossreactivity of Pru p 3 with Jug r 3 (Juglandaceae), Ara h 9 (Fabaceae), Cor a 8 (Betulaceae), Zea m 14 (Poaceae), Cit s 3 (Rutaceae), and Art v 3 (Asteraceae) [27].These IgE-binding cross-reactivities depend on very similar three-dimensional conformations and exposed molecular surfaces.In this respect, the phylogenetic tree built for nsLTPs of different origins shows some rather close relationships of fruit nsLTPs with other seeds and pollen nsLTPs, irrespective of their phylogenetic relationships (Figure 14).In this regard, nsLTP allergens from the Rosaceae family form a very homogeneous group with a high degree of cross-reactivity among the members, e.g., strong cross-reactivity of Pru p 3 with Mal d 3, Pru av 3, and Pru ar 3, and lower cross-reactivity with nsLTP allergens from other families, e.g., low cross-reactivity of Pru p 3 with Jug r 3 (Juglandaceae), Ara h 9 (Fabaceae), Cor a 8 (Betulaceae), Zea m 14 (Poaceae), Cit s 3 (Rutaceae), and Art v 3 (Asteraceae) [27].These IgEbinding cross-reactivities depend on very similar three-dimensional conformations and exposed molecular surfaces.The nsLTP allergens and PR10 Bet v 1-like protein allergens possess internal cavities, which usually accommodate a set of small molecules ligands including unsaturated fatty acids and other lipidic compounds (nsLTPs), polyphenols, and alkaloids (PR10 Bet v 1-like proteins) (Figure 15) [28].In addition to enhancing the stability and eventually modifying the allergenicity of nsLTPs and PR10 Bet v 1-like protein allergens [29][30][31], these small molecule ligands have an adjuvant effect, and participate in the activation and regulation of the allergic and inflammatory responses via the activation of signaling pathways common to innate immunity and allergic responses [32][33][34].

Profilins
Profilins are typical pollen allergens that also occur in the vegetative or and fruits.They correspond to small polypeptides of ⁓130 amino-acid resid a canonical structural organization made of a central β-sheet flanked on b few α-helices (Figure 16A) that gives a rather flattened shape to these mol 16B,C) [35].

Profilins
Profilins are typical pollen allergens that also occur in the vegetative organs of plants and fruits.They correspond to small polypeptides of ~130 amino-acid residues exhibiting a canonical structural organization made of a central β-sheet flanked on both sides by a few α-helices (Figure 16A) that gives a rather flattened shape to these molecules (Figure 16B,C) [35].

Profilins
Profilins are typical pollen allergens that also occur in the vegetative organs of plants and fruits.They correspond to small polypeptides of ⁓ 130 amino-acid residues exhibiting a canonical structural organization made of a central β-sheet flanked on both sides by a few α-helices (Figure 16A) that gives a rather flattened shape to these molecules (Figure 16B,C) [35].This rather loose structural organization, not sustained by disulfide bridges, accounts for a low resistance to thermal denaturation, that explains why profilins lose their allergenic potency after cooking [36].However, despite the cooking of profilin-containing foods destroying their IgE-binding epitopes, the remaining continuous epitopes keep some cross-reactive T cell activity in vitro [37].
Using synthetic peptides spanning the full Cuc m 2 amino acid sequence, the IgEbinding epitopes of the muskmelon profilin, Cuc m 2, were identified to stretches 66-75 + 81-93 (E1) and 95-99 + 121-131 (E2) of the amino acid sequence, which collapses in two patches (E1 and E2) well exposed on the molecular surface of the profilin (Figure 17).An additional stretch corresponding to residues 2-10 + 35-45 forms another discontinuous epitope E3 on the molecular surface of Cuc m 2 [38].A discontinuous mimotope was fur ther identified on the Cuc m 2 surface, corresponding to the sequence stretch 2SW + 5AY + 9DH + 111TPGQ + 116NM + 121RL, which plays a role in the cross-reactivity of Cuc m 2 with pollen profilins [39].This rather loose structural organization, not sustained by disulfide bridges, accounts for a low resistance to thermal denaturation, that explains why profilins lose their allergenic potency after cooking [36].However, despite the cooking of profilin-containing foods destroying their IgE-binding epitopes, the remaining continuous epitopes keep some crossreactive T cell activity in vitro [37].
Using synthetic peptides spanning the full Cuc m 2 amino acid sequence, the IgEbinding epitopes of the muskmelon profilin, Cuc m 2, were identified to stretches 66-75 + 81-93 (E1) and 95-99 + 121-131 (E2) of the amino acid sequence, which collapses in two patches (E1 and E2) well exposed on the molecular surface of the profilin (Figure 17).An additional stretch corresponding to residues 2-10 + 35-45 forms another discontinuous epitope E3 on the molecular surface of Cuc m 2 [38].A discontinuous mimotope was further identified on the Cuc m 2 surface, corresponding to the sequence stretch 2SW + 5AY + 9DH + 111TPGQ + 116NM + 121RL, which plays a role in the cross-reactivity of Cuc m 2 with pollen profilins [39].Similarly, the IgE-binding activity of a recombinant soybean profilin, rGly m 3, is mediated by extended conformational epitopes, since three overlapping profilin fragments comprising amino acid residues 1-65, 38-88, and 50-131 displayed no significant IgE-binding activity towards the sera from soybean allergic patients [40].In addition, plant food profilins and pollen profilins exhibited an equivalent IgE-binding reactivity, which suggests that food and pollen profilins share most of their IgE-binding epitopes [41].
Recently, the distribution of negative charges on the molecular surface of various profilins, including Amb a 4 and Amb a 8 from Ambrosia artemisiifolia, Art v 4 from Artemisia vulgaris, Bet v 2 from Betula verrucosa, Fra e 2 from Fraxinus excelsior, Hev b 8 from Hevea brasiliensis, Phl p 2 from Phleum pratense, and Zea m 12 from Zea mays, was relevant for the recognition of profilins by monoclonal antibodies, and should participate in their IgE-binding cross-reactivity [42].
The alignment of their amino acid sequences shows a high degree of conservation for Similarly, the IgE-binding activity of a recombinant soybean profilin, rGly m 3, is mediated by extended conformational epitopes, since three overlapping profilin fragments comprising amino acid residues 1-65, 38-88, and 50-131 displayed no significant IgEbinding activity towards the sera from soybean allergic patients [40].In addition, plant food profilins and pollen profilins exhibited an equivalent IgE-binding reactivity, which suggests that food and pollen profilins share most of their IgE-binding epitopes [41].
Recently, the distribution of negative charges on the molecular surface of various profilins, including Amb a 4 and Amb a 8 from Ambrosia artemisiifolia, Art v 4 from Artemisia vulgaris, Bet v 2 from Betula verrucosa, Fra e 2 from Fraxinus excelsior, Hev b 8 from Hevea brasiliensis, Phl p 2 from Phleum pratense, and Zea m 12 from Zea mays, was relevant for the recognition of profilins by monoclonal antibodies, and should participate in their IgE-binding cross-reactivity [42].
The alignment of their amino acid sequences shows a high degree of conservation for the profilin allergens, independent of their botanical origin (Figure 18).Accordingly, all these allergens exhibit a very conserved three-dimensional structure.As widely distributed among fruits, seeds and pollens, profilins constitute a very homogenous family of phylogenetically related pan-allergens.The close phylogenetic relationships clearly appear in the phylogenetic tree built from the amino acid sequences of profilins (Figure 19).Consequently, IgE-binding cross-reactivity commonly occurs between apparently distantly related plant sources, e.g., between melon (Cuc m 2) and mugwort (Art v 4), between celery (Api g 4) and chestnut (Cor a 2), between peach (Pru p 4) and birch (Bet v 2), etc. [43].However, due to their weak resistance to heat denaturation (cooking), profilins usually behave as mild allergens.2.5.Gibberellin-Regulated Proteins (GRPs) The GRPs (also known as snakins) sequenced so far share a superposable canonical structural organization made of three short α-helices α1, α2, and α3, tightly linked by two series of three disulfide bonds interconnecting helices α1-α2 and helices α2-α3, respectively (Figure 20A).The resulting three-dimensional structure consists of a dome-shaped molecule, crossed by a deep cleft located at its bottom.Depending on the GRPs, the cleft is enlarged, e.g., in the peach peamaclein Pru p 7 (Figure 20B), or narrower, e.g., in the potato snakin-1.The distribution of electronegatively charged residues Asp and Glu are primarily located at the top of the dome, while other parts of the GRPs, especially the cleft, exhibit an electropositive character, due to the high concentration of electropositive Arg and Lys residues on the molecular surface (Figure 20C).Accordingly, GRPs behave as predominantly basic proteins, displaying a high pI value (calculated pI of 8.52 for Pru p 7).
Allergies 2023, 3, FOR PEER REVIEW primarily located at the top of the dome, while other parts of the GRPs, especial exhibit an electropositive character, due to the high concentration of electrop and Lys residues on the molecular surface (Figure 20C).Accordingly, GRPs predominantly basic proteins, displaying a high pI value (calculated pI of 8.5 7).By virtue of their extremely tightly packed structure, sustained by a high conserved disulfide bridges, peamaclein (Pru p 7) and other GRPs from frui consist of small proteins resistant to digestive proteases and heat denaturat regard, Pru p 7 was shown to become sensitive to the digestive proteolytic d only after heat denaturation [44].
Although no data on the allergenicity of GRPs are available, a hydropho analysis (HCA) of GRPs, together with a surface analysis of the modeled prote one to predict the presence of exposed areas rich in hydrophilic and electropo dues [45,46] that are susceptible to participate in the IgE-binding activity of gens.The three predicted potential IgE-binding epitopic regions are located on of the dome-shaped GRPs, essentially around the cleft, and roughly coincide w positively charged areas (Figure 21).In contrast, no potential IgE-binding area dicted to occur on the dome face opposite to the cleft.In addition, all of the GR share a very similar distribution of these predicted potential IgE-binding ar could account for the cross-reactivity observed between GRP allergens from pollens.By virtue of their extremely tightly packed structure, sustained by a high number of conserved disulfide bridges, peamaclein (Pru p 7) and other GRPs from fruit or pollen consist of small proteins resistant to digestive proteases and heat denaturation.In this regard, Pru p 7 was shown to become sensitive to the digestive proteolytic degradation only after heat denaturation [44].
Although no data on the allergenicity of GRPs are available, a hydrophobic cluster analysis (HCA) of GRPs, together with a surface analysis of the modeled proteins, allows one to predict the presence of exposed areas rich in hydrophilic and electropositive residues [45,46] that are susceptible to participate in the IgE-binding activity of these allergens.The three predicted potential IgE-binding epitopic regions are located on the bottom of the dome-shaped GRPs, essentially around the cleft, and roughly coincide with electropositively charged areas (Figure 21).In contrast, no potential IgE-binding areas were predicted to occur on the dome face opposite to the cleft.In addition, all of the GRP allergens share a very similar distribution of these predicted potential IgE-binding areas, which could account for the cross-reactivity observed between GRP allergens from fruits and pollens.
positively charged areas (Figure 21).In contrast, no potential IgE-binding areas dicted to occur on the dome face opposite to the cleft.In addition, all of the GRP share a very similar distribution of these predicted potential IgE-binding are could account for the cross-reactivity observed between GRP allergens from pollens.The alignment of amino acid sequences of GRP allergens shows a high degree of conservation of GRPs from fruits and pollens (Figure 22).
Allergies 2023, 3, FOR PEER REVIEW 18 The alignment of amino acid sequences of GRP allergens shows a high degree of conservation of GRPs from fruits and pollens (Figure 22).Fruit GRPs constitute a homogeneous group of phylogenetically related proteins, except for GRPs from ananas (Ana co GRP), banana (Mus a GRP) and Japanese plum (Pru m 7), which are less closely related to the fruit clade (Figure 23).In addition, GRP from the Cupressaceae pollen, Cry j 7, Cup s 7 and Jun a 7, are phylogenetically close to the fruit GRPs.Accordingly, it is not surprising that GRPs are highly immunochemically crossreactive allergens from fruit (Pru p 7, Pun g 7) and pollen (Cup s 7, Cry j 7) sources [47,48].Fruit GRPs constitute a homogeneous group of phylogenetically related proteins, except for GRPs from ananas (Ana co GRP), banana (Mus a GRP) and Japanese plum (Pru m 7), which are less closely related to the fruit clade (Figure 23).In addition, GRP from the Cupressaceae pollen, Cry j 7, Cup s 7 and Jun a 7, are phylogenetically close to the fruit GRPs.Accordingly, it is not surprising that GRPs are highly immunochemically crossreactive allergens from fruit (Pru p 7, Pun g 7) and pollen (Cup s 7, Cry j 7) sources [47,48].
Fruit GRPs constitute a homogeneous group of phylogenetically related proteins, except for GRPs from ananas (Ana co GRP), banana (Mus a GRP) and Japanese plum (Pru m 7), which are less closely related to the fruit clade (Figure 23).In addition, GRP from the Cupressaceae pollen, Cry j 7, Cup s 7 and Jun a 7, are phylogenetically close to the fruit GRPs.Accordingly, it is not surprising that GRPs are highly immunochemically crossreactive allergens from fruit (Pru p 7, Pun g 7) and pollen (Cup s 7, Cry j 7) sources [47,48].

Defensins
The plant defensin PR-12 family comprises small acidic molecules of about 110 amino acid residues, exhibiting the canonical knottin fold, a structural αβ motif made of an α-

Defensins
The plant defensin PR-12 family comprises small acidic molecules of about 110 amino acid residues, exhibiting the canonical knottin fold, a structural αβ motif made of an α-helix linked to an antiparallel three-standed β-sheet, stabilized by 3 or 4 disulfide bridges which adopt a disposition known as the so-called "disulfide through disulfide knot" (Figure 24) [49].Genuine defensins, e.g., brazzein from the Pentadiplandra brazzeana, are restricted to the knottin domain, whereas the closely related defensin-like proteins, e.g., Art v 1 from mugwort (Artemisia vulgaris) and Amb a 4 from ragweed (Ambrosia artemisiifolia), possess a knottin domain extended at the C-terminus by a long prolin-rich tail (Figure 25).With the exception of brazzein, a sweet-tasting and taste-modifying protein of fruits from the African shrub Pentadiplandra brazzaeana [50,51], whose the sweetening power is 500 to 2000 times greater than that of sucrose [52], other defensins essentially occur in the pollen (Art v 1, Amb a 4), vegetative parts (defensins from pea (Pisum sativum), and fenugreek (Trigonella foenum-graecum) of the plants.
Allergies 2023, 3, FOR PEER REVIEW 19 helix linked to an antiparallel three-standed β-sheet, stabilized by 3 or 4 disulfide bridges which adopt a disposition known as the so-called "disulfide through disulfide knot" (Figure 24) [49].Genuine defensins, e.g., brazzein from the Pentadiplandra brazzeana, are restricted to the knottin domain, whereas the closely related defensin-like proteins, e.g., Art v 1 from mugwort (Artemisia vulgaris) and Amb a 4 from ragweed (Ambrosia artemisiifolia), possess a knottin domain extended at the C-terminus by a long prolin-rich tail (Figure 25).With the exception of brazzein, a sweet-tasting and taste-modifying protein of fruits from the African shrub Pentadiplandra brazzaeana [50,51], whose the sweetening power is 500 to 2000 times greater than that of sucrose [52], other defensins essentially occur in the pollen (Art v 1, Amb a 4), vegetative parts (defensins from pea (Pisum sativum), and fenugreek (Trigonella foenum-graecum) of the plants.The IgE-binding activity of the pollen defensin allergens A Amb a 4 (Ambrosia artemisiifolia), and Par h 1 (Parthenium hystero of extended defensin-like proteins comprising a N-terminal def amino acid residues associated with a C-terminal proline-rich d residues, was investigated using sera from Austrian, Canadian tients [53].Both domains participated in the defensin allergenici IgE-binding cross-reactivity (proline-rich domain).In fact, stru like proteins are still capable of IgE-binding capacity, suggesting in the defensin domain should be responsible for the IgE-bindin teraction of Art v 1 with the corresponding IgE allowed identific posed amino acid residues (S3, K4, K8, S14, R40, E41, E45, S46, K binding of antibodies (Figure 26) [49].Cross-inhibition experime line-rich domain is also involved in the IgE-binding cross-reactiv The IgE-binding activity of the pollen defensin allergens Art v 1 (Artemisia vulgaris), Amb a 4 (Ambrosia artemisiifolia), and Par h 1 (Parthenium hysterophorus), which all consist of extended defensin-like proteins comprising a N-terminal defensin domain of about 50 amino acid residues associated with a C-terminal proline-rich domain of >60 amino acid residues, was investigated using sera from Austrian, Canadian and Korean allergic patients [53].Both domains participated in the defensin allergenicity (defensin domain) and IgE-binding cross-reactivity (proline-rich domain).In fact, structurally altered defensin-like proteins are still capable of IgE-binding capacity, suggesting that continuous epitopes in the defensin domain should be responsible for the IgE-binding activity (Figure 25).Interaction of Art v 1 with the corresponding IgE allowed identification of some surface exposed amino acid residues (S3, K4, K8, S14, R40, E41, E45, S46, K55, A63) involved in the binding of antibodies (Figure 26) [49].Cross-inhibition experiments showed that the proline-rich domain is also involved in the IgE-binding cross-reactivity, but different degrees of crossreactivity depend on the origin of sera with a high, medium, and low cross-reactivity for sera from Austrian, Canadian and Korean allergic patients, respectively.s 2023, 3, FOR PEER REVIEW of cross-reactivity depend on the origin of sera with a high, me tivity for sera from Austrian, Canadian and Korean allergic pat The alignment of defensin allergens shows a dichotomy amino acid sequences (Figure 27).In addition, the highly cons The alignment of defensin allergens shows a dichotomy between short and long amino acid sequences (Figure 27).In addition, the highly conserved defensins from pollens differ from the rather less conserved defensins from fruits.The alignment of defensin allergens shows a dichotomy between short and amino acid sequences (Figure 27).In addition, the highly conserved defensins from lens differ from the rather less conserved defensins from fruits.The phylogenetic tree built up from the amino acid sequences of plant defensins suggests that possible cross-reactivities and cross-allergies could occur between defensins of pollens, fruits, seeds, and vegetables (Figure 28) [54].
Allergies 2023, 3, FOR PEER REVIEW The phylogenetic tree built up from the amino acid sequences of plant defensins gests that possible cross-reactivities and cross-allergies could occur between defensin pollens, fruits, seeds, and vegetables (Figure 28) [54].According to the discrepancies observed in the amino acid sequences of the diff classes of chitinases, class-I, -III, and -IV chitinases cluster in three distinct groups phylogenetic tree built from the amino acid sequence alignment (Figure 32).Depen on these phylogenetic relationships, some cross-reactivity should be predicted amon chitinases from class-I group, whereas no cross-reactivity should occur between chiti from the class-I and class-III groups.In this respect, class-I chitinases, which are c related to Hev b 11, should participate in the latex-fruit syndrome [58].According to the discrepancies observed in the amino acid sequences of the different classes of chitinases, class-I, -III, and -IV chitinases cluster in three distinct groups in the phylogenetic tree built from the amino acid sequence alignment (Figure 32).Depending on these phylogenetic relationships, some cross-reactivity should be predicted among the chitinases from class-I group, whereas no cross-reactivity should occur between chitinases from the class-I and class-III groups.In this respect, class-I chitinases, which are closely related to Hev b 11, should participate in the latex-fruit syndrome [58].

PR2 Glucanases
The β1,4-endoglucanases from higher plants all exhibit the α8β 8 β-barrel organization, made of an internal crown of eight β-strands connected by loops to a periphera crown of eight α-helices.A short additional β-hairpin completes the three-dimensiona structure (Figure 33).A long catalytic groove centered on the β-strand crown contains the active site responsible for the cleavage of the β1,4-linked glucan chains by plant glucanases.This three-dimensional organization pattern is conserved in all the β1,3-endoglucanases from higher plants, especially in the glucanase allergens identified so far, e.g.Mus a 5 from banana [7], Pru av 2 cherry [59], VVTL-1 from grape [60], and Hev b 2 from the latex of rubber tree [61].Chitinases from fruits, seeds, pollen, and latex are highlighted in red, yellow, green, and white, respectively.

PR2 Glucanases
The β1,4-endoglucanases from higher plants all exhibit the α 8 β 8 β-barrel organization, made of an internal crown of eight β-strands connected by loops to a peripheral crown of eight α-helices.A short additional β-hairpin completes the three-dimensional structure (Figure 33).A long catalytic groove centered on the β-strand crown contains the active site responsible for the cleavage of the β1,4-linked glucan chains by plant glucanases.This three-dimensional organization pattern is conserved in all the β1,3-endoglucanases from higher plants, especially in the glucanase allergens identified so far, e.g., Mus a 5 from banana [7], Pru av 2 cherry [59], VVTL-1 from grape [60], and Hev b 2 from the latex of rubber tree [61].Chitinases from fruits, seeds, pollen, and latex are highlighted in red, yellow green, and white, respectively.

PR2 Glucanases
The β1,4-endoglucanases from higher plants all exhibit the α8β 8 β-barrel organiza tion, made of an internal crown of eight β-strands connected by loops to a periphera crown of eight α-helices.A short additional β-hairpin completes the three-dimensiona structure (Figure 33).A long catalytic groove centered on the β-strand crown contains the active site responsible for the cleavage of the β1,4-linked glucan chains by plant glu canases.This three-dimensional organization pattern is conserved in all the β1,3-endoglu canases from higher plants, especially in the glucanase allergens identified so far, e.g.Mus a 5 from banana [7], Pru av 2 cherry [59], VVTL-1 from grape [60], and Hev b 2 from the latex of rubber tree [61].Probing synthetic peptides spanning the amino acid sequence of Hev b 2 with specific IgE-containing sera from allergic patients allowed identification of up to eight continuous IgE-binding epitopes.They correspond to different stretches of amino acid sequences arrayed on the molecular surface, essentially located on the back face opposite to the catalytic groove [62] (Figure 34A,B).Compared to Hev b 2, the continuous IgE-binding epitopic regions identified in the banana glucanase Mus a 5, are similarly located on the back face of the allergen, and exhibit a very similar conformation on the molecular surface [7] (Figure 34C,D).The similar distribution and conformation of the epitopic regions in both allergens suggests the occurrence of a rather high degree of cross-allergenicity between these fruit and latex allergens that should participate in the latex-fruit syndrome [58].
, 3, FOR PEER REVIEW 25 back face of the allergen, and exhibit a very similar conformation on the molecular surface [7] (Figure 34C,D).The similar distribution and conformation of the epitopic regions in both allergens suggests the occurrence of a rather high degree of cross-allergenicity between these fruit and latex allergens that should participate in the latex-fruit syndrome [58].The glucanase allergens exhibit a high degree of identity in the multiple alignments of their amino acid sequences (Figure 35).With the exception of Ole e 9 [63], the olive tree allergen, which differs from other fruit and seed glucanases by a quite different amino acid sequence and an additional long C-terminal expansion, other fruit allergens display closely related amino acid sequences.Accordingly, they are distributed in two closely related clusters; the first cluster groups Hev b 2, Rosaceae and other fruit allergens, and the second cluster contains most of the seed glucanase allergens.Another heterogeneous cluster contains Ole e 9, Mus 5, and other Solanaceae glucanases (Figure 36).The close vicinity of these different clusters points out the phylogenetic relationships that should occur among the fruit and seed allergens, and between Hev b 2 and fruit glucanase allergens (latex-fruit syndrome).The glucanase allergens exhibit a high degree of identity in the multiple alignments of their amino acid sequences (Figure 35).With the exception of Ole e 9 [63], the olive tree allergen, which differs from other fruit and seed glucanases by a quite different amino acid sequence and an additional long C-terminal expansion, other fruit allergens display closely related amino acid sequences.Accordingly, they are distributed in two closely related clusters; the first cluster groups Hev b 2, Rosaceae and other fruit allergens, and the second cluster contains most of the seed glucanase allergens.Another heterogeneous cluster contains Ole e 9, Mus 5, and other Solanaceae glucanases (Figure 36).The close vicinity of these different clusters points out the phylogenetic relationships that should occur among the fruit and seed allergens, and between Hev b 2 and fruit glucanase allergens (latex-fruit syndrome).

Seed Storage Proteins from Fruit Kernels
Botanically, fruits from higher plants consist of seed-bearing structures derived from the gynecium of the flowers.Accordingly, mature edible fruits contain seeds that correspond to the modified ovules of the flowers.Depending on the size of the seeds distributed in the flesh, additional allergic manifestations could result from seed allergens different from the pericarp allergens, when seeds are consumed together with the pericarp, e.g., in the case of small sized seed-containing fruits like kiwi, tomato, grape, or the Japanese lantern cherry (Physalis alkekengi) [64].In addition, seeds or seed fragments of fruits from the Rosaceae (apple, pear) or the Rutaceae (lemon, orange) families can be accidentally consumed, and cause unexpected allergenic manifestations [5].
Seed cupin allergens possess the canonical homotrimeric organization of their monomers, containing two cupin motifs in a flat-shaped structure (Figure 37A,B).A single homotrimer builds the 7S globulins, whereas two homotrimers associate face to face to build the 11S globulins or legumin allergens.The non-covalent association of two ho-motrimers mainly results from electrostatic interactions occurring between the opposite electropositive and electronegative charges of both faces of each homotrimers (Figure 37C).
Seed cupin allergens possess the canonical homotrimeric organization of th omers, containing two cupin motifs in a flat-shaped structure (Figure 37A,B).A s motrimer builds the 7S globulins, whereas two homotrimers associate face to face the 11S globulins or legumin allergens.The non-covalent association of two hom mainly results from electrostatic interactions occurring between the opposite ele tive and electronegative charges of both faces of each homotrimers (Figure 37C).Due to the conservation of both the amino acid sequences and three-dim structures of the 11S globulin allergens, a few continuous IgE-binding epitopic have been identified as common IgE-binding epitopes shared by the seed 11S allergens [68].These well-exposed, common IgE-binding epitopic regions also occ 11S globulin allergens Act d 12 from kiwi fruit and Fra v-Leg from strawberry vesca) (Figure 38), and could thus account for some allergic response in sensitize [69,70].The alignment of amino acid sequences of 11S globulin/legumin allergens e high degree of conservation with, however, some discrepancies, due to insertions events that have occurred in the medium part of the sequences (Figure 39).These s homologies are in agreement with the very conserved three-dimensional structur seed storage proteins.The phylogenetic tree built from the amino acid sequence a of 11S globulins shows that the clustering of fruit and seed 11S globulins roug cides with their distribution in the phylogenetically related families of higher pla ure 40).Due to the conservation of both the amino acid sequences and three-dimensional structures of the 11S globulin allergens, a few continuous IgE-binding epitopic regions have been identified as common IgE-binding epitopes shared by the seed 11S globulin allergens [68].These well-exposed, common IgE-binding epitopic regions also occur in the 11S globulin allergens Act d 12 from kiwi fruit and Fra v-Leg from strawberry (Fragaria vesca) (Figure 38), and could thus account for some allergic response in sensitized people [69,70].
omers, containing two cupin motifs in a flat-shaped structure (Figure 37A,B).A s motrimer builds the 7S globulins, whereas two homotrimers associate face to face the 11S globulins or legumin allergens.The non-covalent association of two hom mainly results from electrostatic interactions occurring between the opposite ele tive and electronegative charges of both faces of each homotrimers (Figure 37C).Due to the conservation of both the amino acid sequences and three-dim structures of the 11S globulin allergens, a few continuous IgE-binding epitopi have been identified as common IgE-binding epitopes shared by the seed 11S allergens [68].These well-exposed, common IgE-binding epitopic regions also oc 11S globulin allergens Act d 12 from kiwi fruit and Fra v-Leg from strawberry vesca) (Figure 38), and could thus account for some allergic response in sensitize [69,70].The alignment of amino acid sequences of 11S globulin/legumin allergens e high degree of conservation with, however, some discrepancies, due to insertions events that have occurred in the medium part of the sequences (Figure 39).These homologies are in agreement with the very conserved three-dimensional structur seed storage proteins.The phylogenetic tree built from the amino acid sequence a of 11S globulins shows that the clustering of fruit and seed 11S globulins roug cides with their distribution in the phylogenetically related families of higher pla ure 40).The alignment of amino acid sequences of 11S globulin/legumin allergens exhibits a high degree of conservation with, however, some discrepancies, due to insertions/deletion events that have occurred in the medium part of the sequences (Figure 39).These sequence homologies are in agreement with the very conserved three-dimensional structure of these seed storage proteins.The phylogenetic tree built from the amino acid sequence alignment of 11S globulins shows that the clustering of fruit and seed 11S globulins roughly coincides with their distribution in the phylogenetically related families of higher plants (Figure 40).The germin and germin-like allergens possess a single homotrimeric similar to that of vicilins, but differ from typical vicilins by their monomers, a single cupin motif.Accordingly, the homotrimeric organization of germ two superposerd dimers of homotrimers (Figure 41A,B).Like in other cupin faces of the homotrimeric germins and germin-like allergens exhibit a com tion of electrostatic charges (Figure 41C) [71].The germin and germin-like allergens possess a single homotrimeric organization similar to that of vicilins, but differ from typical vicilins by their monomers, which contain a single cupin motif.Accordingly, the homotrimeric organization of germins consists of two superposerd dimers of homotrimers (Figure 41A,B).Like in other cupin proteins, both faces of the homotrimeric germins and germin-like allergens exhibit a complex distribution of electrostatic charges (Figure 41C) [71].To date, with the exception of Cis s 1, the allergenic potential of germin-like proteins has not been clearly demonstrated.In addition, the localization of IgE-binding epitopes of germin-like protein allergens has not been investigated but IgE-binding experiments, ELISA inhibition assays and in vivo skin prick tests (SPT) performed with sera from allergic patients showed that previous deglycosylation of Cis s 1 resulted in an important loss of IgE-binding reactivity [66,72].These results suggest that N-glycans play a prominent role in the IgE-binding capacity of Cit s 1.In fact, the Cit s 1 allergen corresponds to a heavily N-glycosylated allergen, which contains several putative N-glycosylation sites.Assuming these N-glycans are occupied by N-glycans of the complex type allowed us to build a heavily glycosylated Cit s 1 allergen, using the GlyProt server facilities (Figure 42).These predictions agree with the IgE-binding results observed with Cit s 1 [72].The comparison of amino acid sequences of germin-like proteins shows a rather moderate degree of conservation (Figure 43), in spite of a very conserved three-dimensional conformation.According to this amino acid sequence heterogeneity, the phylogenetic tree of germins and germin-like proteins built from the amino acid sequence alignment, shows a rather marked dispersion of the allergens, even though fruit and seed germin-like allergens exhibit a tendency to clustering in well-individualized phylogenetically related groups (Figure 44).To date, with the exception of Cis s 1, the allergenic potential of germin-like proteins has not been clearly demonstrated.In addition, the localization of IgE-binding epitopes of germin-like protein allergens has not been investigated but IgE-binding experiments, ELISA inhibition assays and in vivo skin prick tests (SPT) performed with sera from allergic patients showed that previous deglycosylation of Cis s 1 resulted in an important loss of IgE-binding reactivity [66,72].These results suggest that N-glycans play a prominent role in the IgE-binding capacity of Cit s 1.In fact, the Cit s 1 allergen corresponds to a heavily N-glycosylated allergen, which contains several putative N-glycosylation sites.Assuming these N-glycans are occupied by N-glycans of the complex type allowed us to build a heavily glycosylated Cit s 1 allergen, using the GlyProt server facilities (Figure 42).These predictions agree with the IgE-binding results observed with Cit s 1 [72].To date, with the exception of Cis s 1, the allergenic potential of germin-like p has not been clearly demonstrated.In addition, the localization of IgE-binding epit germin-like protein allergens has not been investigated but IgE-binding exper ELISA inhibition assays and in vivo skin prick tests (SPT) performed with sera from gic patients showed that previous deglycosylation of Cis s 1 resulted in an import of IgE-binding reactivity [66,72].These results suggest that N-glycans play a pro role in the IgE-binding capacity of Cit s 1.In fact, the Cit s 1 allergen correspon heavily N-glycosylated allergen, which contains several putative N-glycosylatio Assuming these N-glycans are occupied by N-glycans of the complex type allowe build a heavily glycosylated Cit s 1 allergen, using the GlyProt server facilities (Fig These predictions agree with the IgE-binding results observed with Cit s 1 [72].The comparison of amino acid sequences of germin-like proteins shows a rathe erate degree of conservation (Figure 43), in spite of a very conserved three-dime conformation.According to this amino acid sequence heterogeneity, the phylogene of germins and germin-like proteins built from the amino acid sequence alignment a rather marked dispersion of the allergens, even though fruit and seed germin-lik gens exhibit a tendency to clustering in well-individualized phylogenetically groups (Figure 44).The comparison of amino acid sequences of germin-like proteins shows a rather moderate degree of conservation (Figure 43), in spite of a very conserved three-dimensional conformation.According to this amino acid sequence heterogeneity, the phylogenetic tree of germins and germin-like proteins built from the amino acid sequence alignment, shows a rather marked dispersion of the allergens, even though fruit and seed germin-like allergens exhibit a tendency to clustering in well-individualized phylogenetically related groups (Figure 44).The extreme diversity of allergens in some fruits like kiwi and tomato (Figure 45) illustrates another aspect often observed in fruit allergies.A great number of fruit allergens consist of pan-allergens widely distributed in different plant organs, including fruits, seeds, pollen and other vegetative parts of plants.According to such a distribution, a high degree of cross-reactivity often occurs between fruits, seeds, and pollen from apparently distantly related plant species.The extreme diversity of allergens in some fruits like kiwi and tomato (Figure 45) illustrates another aspect often observed in fruit allergies.A great number of fruit allergens consist of pan-allergens widely distributed in different plant organs, including fruits, seeds, pollen and other vegetative parts of plants.According to such a distribution, a high degree of cross-reactivity often occurs between fruits, seeds, and pollen from apparently distantly related plant species.In addition to proteins belonging to the different PR-protein families, such as the P 10 Bet v 1 like proteins or the PR-14 LTPs, which have become prominent fruit allerge in recent years, other fruit allergens, less known because they are less investigated, su as the PR-5 TLPs [11,73], defensins and defensin-like proteins with a knottin fold [74], an GRPs [45], are now considered as important emerging fruit allergens.

Cross-Reactivity of Fruit Allergens
Most of the fruit allergens consist of pan-allergens widely distributed in differe plant organs, which are thus responsible for cross-reactivities associated or not to cros allergenicities between fruits, vegetables, and pollens.All these fruit allergens exhib closely related amino acid sequences and quasi-identical folds.Fruit and pollen profili display a high degree of cross-reactivity (Figure 46A) [43].Depending on the relatedne between their amino acid sequences, their overall fold, and their IgE-binding epitope com munity, nsLTP allergens from Rosaceae fruits, especially Pru p 3, display pronounce cross-reactivities with other nsLTPs from vegetable, seed and pollen sources (Figure 46 [11,15,27].Due to the widespread distribution of Bet v 1-like proteins closely related to th white birch pollen Bet v 1 allergen in various tree pollens, vegetables, and seeds, extensi IgE-binding cross-reactivity between the pollen of various trees and foods are frequent observed (Figure 46D) [19].Although less investigated as compared to profilins and PR Bet v 1-like proteins, the recently identified GRP allergens fall apparently into the sam category of highly cross-reactive allergens (Figure 46C).In addition to proteins belonging to the different PR-protein families, such as the PR-10 Bet v 1 like proteins or the PR-14 LTPs, which have become prominent fruit allergens in recent years, other fruit allergens, less known because they are less investigated, such as the PR-5 TLPs [11,73], defensins and defensin-like proteins with a knottin fold [74], and GRPs [45], are now considered as important emerging fruit allergens.

Cross-Reactivity of Fruit Allergens
Most of the fruit allergens consist of pan-allergens widely distributed in different plant organs, which are thus responsible for cross-reactivities associated or not to cross-allergenicities between fruits, vegetables, and pollens.All these fruit allergens exhibit closely related amino acid sequences and quasi-identical folds.Fruit and pollen profilins display a high degree of cross-reactivity (Figure 46A) [43].Depending on the relatedness between their amino acid sequences, their overall fold, and their IgE-binding epitope community, nsLTP allergens from Rosaceae fruits, especially Pru p 3, display pronounced cross-reactivities with other nsLTPs from vegetable, seed and pollen sources (Figure 46B) [11,15,27].Due to the widespread distribution of Bet v 1-like proteins closely related to the white birch pollen Bet v 1 allergen in various tree pollens, vegetables, and seeds, extensive IgE-binding cross-reactivity between the pollen of various trees and foods are frequently observed (Figure 46D) [19].Although less investigated as compared to profilins and PR10 Bet v 1-like proteins, the recently identified GRP allergens fall apparently into the same category of highly cross-reactive allergens (Figure 46C).The cross-reactivities between fruit allergens and homologous proteins from other vegetable, seed, and pollen sources account for different food-pollen syndromes.The allergens responsible for these food-pollen syndromes essentially consist of the pan-allergens nsLTPs, profilins, GRPs, β-1,3-glucanases, but also the seed storage proteins [4].Most often these pollen-food syndromes result in a benign oral allergy syndrome (OAS) but can, less frequently, lead to a more severe systemic reaction, especially with the peach nsLTP Pru p 3 [27,75].
However, it should be noted that many IgE-binding cross-reactivities measured in vitro by IgE-binding activity tests, inhibition tests of the IgE-binding activity and, more rarely, by the direct activation of previously sensitized basophil cells (BAT), have apparently no clinical significance, and the consumption of fruit or vegetable allergens does not induce any reaction in sensitized individuals [75].

Brief Clinical Aspects
Most of the fruit pan-allergens, like profilins, PR10 Bet v 1-like proteins, nsLTP, and GRPs, are non-sensitizing allergens, which trigger allergic reactions if a previous contact with a sensitizing homologous allergen has occurred.As an example, people sensitized by inhalation of the pollen Bet v 1 allergen can trigger an allergic reaction after eating fruits containing a cross-reactive Bet v 1 homologous allergen, e.g., peach (Pru p 1) or apple (Mal d 1).In this case, the cross-reactivity between pollen allergens and the homologous fruit or vegetable allergens has a clinical expression, which consists of various OAS syndromes induced by fruits and vegetables containing allergens as varied as profilins, PR10 Bet v 1-like proteins, PR14 nsLTPs, GRPs, PR2 β1,3-glucanases, cysteine-proteases, and seed storage proteins [27,75,76].
However, in addition this non-sensitizing character, some fruit allergens, such as the nsLTPs from peach (Pru p 3) [27], maize (Zea m 14) [77], and seed storage proteins like 2S albumins (Cor a 14) and 11S globulin (Cor a 9) [76], can act as sensitizers and trigger more or severe systemic reactions.These discrepancies in the behavior of fruit allergens depend on various factors, including: - The structural characteristics of the allergens (allergens exhibiting tightly packed conformation vs. allergens loosely structured) and some structure-associated properties, like the resistance to digestive proteases and heat (cooking) denaturation [36].
In this respect, fruit nsLTPs, like Pru p 3 and Mal d 3, possess a tightly packed three-dimensional conformation stabilized by four disulfide bridges, the so-called "saxophone-like conformation", that contributes to the extreme resistance of these protein to the degradation by digestive proteases and to heat denaturation [22].Similarly, the tightly packed GRPs offer an enhanced resistance to protease and heat denaturation [44].Conversely, profilins, such as Pru p 4 and Mal d 4, are more loosely structured proteins and offer a weak resistance to both enzymatic digestion and heat denaturation [36]; - The localization and accessibility of the allergens.In this regard, the surface localization of Pru p 3 in the fuzzy covering the peel of the peach [78], and the localization of Mal d 3 in the external cell layers forming the peel of the apple [24], favors the contact of the allergens with the body.Accordingly, removing the peel from peaches and apples is sufficient to eliminate most of the allergens from the fruits; - The amounts of allergens present in fruits and vegetables.This is an important point to consider, because many fruit allergens consist of PR proteins which interfere with the defense of plants against phytopathogenic fungi, bacteria, and viruses [79].In addition, their synthesis can vary considerably, depending on the response of the plant to abiotic stresses, for example water stress or heat stress [80].Moreover, large variations in the allergen content of fruits were measured in different varieties or cultivars of peach and apple [81][82][83].Large variations were also measured in the allergen content of fruits, depending on the cultivation conditions [84,85], the degree of ripening of the harvested fruits, and the shelf life of the postharvest fruit storage [86][87][88][89][90].Other factors can influence the sensitizing propensity of the allergens, such as the route of exposure [76] and the processing of fruits and fruit products before consumption [91].
In addition, some geographical discrepancies have been identified concerning, for example, severe systemic reactions to Pru p 3 in people from Mediterranean countries, compared to people from northern countries [92,93].However, the risk for a severe allergic response to Pru p 3, was also reported for people living outside the Mediterranean countries [15,94].The high resistance of Pru p 3 to both the digestive and thermal degradation largely accounts for the dangerous character of this nsLTP [22].
In general, most of the allergic symptoms following fruit consumption consist of oropharyngeal symptoms, the so-called OAS, which usually occurs very quickly after fruit consumption.The OAS includes various symptoms, but broadly consists of itch, tearing, sneezing, lips swelling and, more rarely, difficulty swallowing, hives, and urticaria.However, severe systemic symptoms can occur after consumption of nsLTP-containing fruits, namely peach Pru p 3, which can require adrenaline injection(s) and emergency hospital care [27,75].A more detailed description of the clinical aspects of allergy to profilins, nsLTPs, PR10 Bet v 1-like proteins, GRPs, and other allergens from fruits and vegetables, is available in the recently published second edition of the Molecular Allergy User's Guide (MAUG2) [95].Clinical aspects of Rosaceae fruit allergies and defensinrelated food allergies are presented in some recently published reviews [12,54].

Comparative Prevalence and Harmful Properties of Fruit Allergens
Fruit allergens responsible for the most frequent allergic manifestations correspond to a rather limited number of structurally well-identified protein families, including profilins, GRPs, defensins, and various PR-protein families such as nsLTPs (PR14), Betv v 1-like PR10, TLPs (PR5), chitinases (PR3) and endoβ1,3-glucanases (PR2) [3].The relationship between the structure and harmfulness of fruit allergens is a complex matter, which apparently depends on both intrinsic and extrinsic factors.
The availability of fruit allergens for the sensitization and subsequent elicitation of an allergic reaction in sensitized people requires both their solubilization and structural preservation during the digestion process.Depending on their structure and physicochemical properties, fruit allergens can readily differ in this respect [96].This ability to resist protease denaturation is also valid in case of dermal sensitization because all tissues contain trypsin-like proteases that can degrade allergens.Furthermore, even in case of proteolytic degradation, the allergens most resistant to denaturation can release peptide fragments capable of retaining some of the allergenicity and harmfulness of native allergens [76].Moreover, the cross-reactivity between fruit allergens and pollen homologous allergens that explains many cases of prior inhalation sensitization observed in allergies to peach and apple could also participate in their harmful character [97].Recently, the ability of Pru p 3 to interact with its natural lipidic ligand camptothecin, associated with phytosphingosine, has been characterized as an adjuvant for promoting the sensitization to Pru p 3 [97].Previously, the interaction of Pru p 3 with free fatty acids had been shown to enhance the Pru p 3 IgE-binding activity [29].
Among the extrinsic factors, the localization and abundancy of allergens in the fruits is another important factor for their availability.As an example, the localization and abundancy of Pru p 3 in the fuzzy covering the peach, and Mal d 3 in the cuticular cell layers of apple [97,98], favor the allergenicity and harmful properties of Rosaceae fruits [98].Other relevant factors, such as the existence of a marked atopic terrain or ethnic predispositions, are to be considered.
Depending on all the factors that contribute to the allergenic potential and harmful potential of fruit allergens, the prevalence and severity of fruit allergies in Europe, in particular, can vary widely between countries.According to [99], the overall prevalence of fruit allergies varies between 0.1% and 4.3%.The most frequent fruit sensitizations in Europe concern peach (7.9%), apple (6.5%), and kiwi fruit (5.2%) [100,101].In northern countries and Italy, apple allergy is frequently mild and depends on prior sensitization to birch pollen PR10 Bet v 1-like protein allergen homologous to Mal d 1, whereas in Spain, sensitization to apple allergen Mal d 3 (nsLTP) predominates in Mediterranean countries and frequently provokes severe anaphylaxis responses [92,[102][103][104].Similarly, allergen sensitization patterns to kiwi fruit differ across Europe [105].Sensitization to kiwi fruit mostly depends on Act d 8 (PR10 Bet v 1-like protein allergen) in western, central and eastern Europe, while it depends on Act d 9 (profilin) and Act d 10 (nsLTP) in southern Europe.Symptoms range from mild OAS (Act d 8, Act d 9) to severe systemic reactions (Act d 10).In general, allergy to fruit profilins is mild, and results in OAS symptoms.Sensitization to fruit profilins is evenly distributed in Europe, but is more frequent in Mediterranean countries, particularly in Spain [106].Sensitization to Pru p 7, the GRP from peach, was reported as the predominant cause of severe cypress pollen-associated peach allergies in southern France [107].Recently, a high prevalence of mango allergy was reported from a self-reported food allergy survey performed in Jiangxi (China), but no indication is available on the causative allergen(s) [108] 6. Conclusions Some general conclusions can be drawn from this review on fruit allergens: 1.
Allergens from edible fruits, especially fleshy fruits, correspond to pan-allergens that are widely distributed in vegetables, seeds, and pollen from other apparently unrelated plants; 2.
Fruit allergens essentially belong to different PR-proteins, which play a role in the defense of plants against phytopathogenic fungi, bacteria, and viruses.Accordingly, their biosynthesis is largely influenced by the stress conditions to which the plants are subjected.The allergen content of fruits can also vary considerably, depending on the ripening stage and storage conditions of the fruits after harvest; 3.
The different families of allergenic PR-proteins exhibit highly conserved amino acid sequences and three-dimensional structures and display close phylogenetic relationships; 4.
Depending on their large distribution and their sequential, structural, and phylogenetical relationships, a high degree of cross-reactivity usually occurs between allergens from fruits and the counterparts from other sources like vegetables, seeds, or pollens.This cross-reactivity is at the origin of various clinical syndromes including, e.g., the apple-birch syndrome, the peach-cypress syndrome, and the peach-latex syndrome.However, many cross-sensitizations with pollen allergens are not clinically relevant; 5.
The allergenicity and toxicity vary largely among fruit allergens.Although all fruit allergens are potentially dangerous, some of them, like nsLTPs and GRPs, are responsible for food allergies, and provoke severe systemic reactions, especially in Mediterranean countries; 6.
In general, the consumption of allergen-containing fruits only results in mild oropharyngeal symptoms that corresponds to the so-called oral allergic syndrome, OAS.In some cases, however, more severe systemic reactions can develop, especially upon consumption of Rosaceae fruits or kiwi fruit.

Bioinformatics
The amino acid sequences of fruit allergens were retrieved from the WHO/IUIS database and the nr NCBI database (accessed on 18 August 2022).Multiple amino acid sequence alignments were performed with ClustalX [109].Unrooted phylogenetic trees were built from the multiple amino acid sequence alignments using the neighbor joining method, and were represented with TreeView [110].
Homology modeling of other allergens, including the potential allergens from the tomato fruit, was performed with the YASARA Structure program [113] using various threedimensional structures of the PDB as templates.PROCHECK [114], ANOLEA [115], and the calculated QMEAN scores [116,117] were used to assess the geometric and thermodynamic qualities of the three-dimensional models.
The surface electrostatic potentials were calculated and rendered with YASARA, using the Amber96 forcefield with dielectric constants applied to the protein and the solvent fixed at 4.0 and 80.0, respectively.Electrostatic potentials were displayed on the molecular surface as red (electro-negatively charged) and blue (electro-positively charged) patches.Neutral surfaces are white.
Continuous (sequential) and discontinuous (conformational) IgE-binding epitopes identified in various publications were mapped on the molecular surface of the corresponding X-ray-solved or modelled fruit allergens.

Figure 1 .
Figure 1.(a) Apple flower showing the central pistil surrounded by the stam the fruit showing the different parts of the apple fruit including the epicarp the mesocarp (M) delineated by a black dashed line, and the sclerified endoca seeds (S).

Figure 1 .
Figure 1.(a) Apple flower showing the central pistil surrounded by the stamens.(b) Cut-section of the fruit showing the different parts of the apple fruit including the epicarp (Ep), the exocarp (Ex), the mesocarp (M) delineated by a black dashed line, and the sclerified endocarp (En) containing the seeds (S).

Figure 2 .
Figure 2. Overview of the different structural scaffolds and distributions of the different families of fruit allergens.

Figure 3 .
Figure 3. (A).Ribbon diagram of Mus a 4 built from three a central β-sandwich domain with an α-helical domain II and a β-hairpin domain III.Up to 8 disulfide bridges (colo tribute to the stability of the three-dimensional structure.(B).Molecular surface of yellow line delineates the cleft occurring between domains I and II.(C).Electrostatic p played on the molecular surface of Mus a 4, showing the electronegative character of th (delineated by a yellow line).Electronegative and electropositive regions are colored respectively, and neutral regions are colored grey.

Figure 3 .
Figure 3. (A).Ribbon diagram of Mus a 4 built from three a central β-sandwich domain (I) associated with an α-helical domain II and a β-hairpin domain III.Up to 8 disulfide bridges (colored red) contribute to the stability of the three-dimensional structure.(B).Molecular surface of Mus a 4. The yellow line delineates the cleft occurring between domains I and II.(C).Electrostatic potentials displayed on the molecular surface of Mus a 4, showing the electronegative character of the central cleft (delineated by a yellow line).Electronegative and electropositive regions are colored red and blue, respectively, and neutral regions are colored grey.

Figure 4 .
Figure 4. IgE-binding epitopic regions predicted on the molecular surface of the TLP from sa fruit (a) and Cup s 3, the green cypress pollen TLP allergen (b).The IgE-binding allergens ferently colored.The electronegative catalytic cleft is highlighted with a yellow line.

Figure 4 .
Figure 4. IgE-binding epitopic regions predicted on the molecular surface of the TLP from sapodilla fruit (a) and Cup s 3, the green cypress pollen TLP allergen (b).The IgE-binding allergens are differently colored.The electronegative catalytic cleft is highlighted with a yellow line.

Figure 7 .Figure 7 .
Figure 7. (A) Ribbon diagram of Pru av 1 built from a bundle of β-sheet associated with α-he N and C correspond to the N-and C-terminal ends of the polypeptide chain, respectively.(B) lecular surface of Pru av 1. (C) Electrostatic potentials displayed on the molecular surface of P 1 (electronegative and electropositive regions are colored red and blue, respectively, and ne regions are colored grey).

Figure 8 .
Figure 8. (A) Ribbon diagram of Pru av 1 showing the P-loop (colored red) containing the key am acid E45 (colored green).(B) Molecular surface of Pru av 1 showing the P-loop region (colored r containing the key residue E45 (colored green).Other key residues N28 and P108 involved in allergenicity of Pru av 1 are colored green.

Figure 8 .
Figure 8. (A) Ribbon diagram of Pru av 1 showing the P-loop (colored red) containing the key amino acid E45 (colored green).(B) Molecular surface of Pru av 1 showing the P-loop region (colored red) containing the key residue E45 (colored green).Other key residues N28 and P108 involved in the allergenicity of Pru av 1 are colored green.

Figure 8 .
Figure 8. (A) Ribbon diagram of Pru av 1 showing the P-loop (colored red) containing the key amino acid E45 (colored green).(B) Molecular surface of Pru av 1 showing the P-loop region (colored red containing the key residue E45 (colored green).Other key residues N28 and P108 involved in the allergenicity of Pru av 1 are colored green.

Figure 11 .
Figure 11.(A) Ribbon diagram of Pru p 3 built from five α-helices α1-α5 (colored orange), linked by 4 disulfide bridges (colored red).N and C correspond to the N-and C-terminal ends of the polypeptide chain, respectively.(B) Molecular surface of Pru p 3. (C) Electrostatic potentials displayed on the molecular surface of Pru p 3 (electronegative and electropositive regions are colored red and blue, respectively, and neutral regions are colored grey).

Figure 11 .
Figure 11.(A) Ribbon diagram of Pru p 3 built from five α-helices α1-α5 (colored orange), linked by 4 disulfide bridges (colored red).N and C correspond to the N-and C-terminal ends of the polypeptide

Figure 11 .
Figure 11.(A) Ribbon diagram of Pru p 3 built from five α-helices α1-α5 (color 4 disulfide bridges (colored red).N and C correspond to the N-and C-terminal tide chain, respectively.(B) Molecular surface of Pru p 3. (C) Electrostatic po the molecular surface of Pru p 3 (electronegative and electropositive regions blue, respectively, and neutral regions are colored grey).

Figure 12 .
Figure 12. (A) Superposition of Pru p 3 (colored pink) and Mal d 3 (colored gre conserved overall conformation of the Prunoideae nsLTP.The conserved dis ored red.B,C.Localization of the IgE-binding epitopic regions 1 (colored red) 3 (colored green) on the molecular surface of Pru p 3 (B) and Mal d 3 (C).(D amino acid sequences of Pru p 3 and Mal d 3, showing the similar localizatio stretches 1 (highlighted in red), 2 (highlighted in blue), and 3 (highlighted in quences.

Figure 12 .
Figure 12. (A) Superposition of Pru p 3 (colored pink) and Mal d 3 (colored green), showing the very conserved overall conformation of the Prunoideae nsLTP.The conserved disulfide bonds are colored red.(B,C) Localization of the IgE-binding epitopic regions 1 (colored red), 2 (colored blue) and 3 (colored green) on the molecular surface of Pru p 3 (B) and Mal d 3 (C).(D) Comparison of the amino acid sequences of Pru p 3 and Mal d 3, showing the similar localization of the IgEbinding stretches 1 (highlighted in red), 2 (highlighted in blue), and 3 (highlighted in green) along the sequences.

Figure 16 .
Figure 16.(A) Ribbon diagram of Cuc m 2 built from a central bundle of β-sheet surrounded by a helices α1-α3.N and C correspond to the N-and C-terminal ends of the polypeptide chain, respec tively.(B) Molecular surface of Cuc m 2. (C) Electrostatic potentials displayed on the molecular surface of Cuc m 2 (electronegative and electropositive regions are colored red and blue, respec tively, and neutral regions are colored grey).

Figure 16 .
Figure 16.(A) Ribbon diagram of Cuc m 2 built from a central bundle of β-sheet surrounded by a-helices α1-α3.N and C correspond to the N-and C-terminal ends of the polypeptide chain, respectively.(B) Molecular surface of Cuc m 2. (C) Electrostatic potentials displayed on the molecular surface of Cuc m 2 (electronegative and electropositive regions are colored red and blue, respectively, and neutral regions are colored grey).

Figure 20 .
Figure 20.(A) Ribbon diagram of the modelled Pru p 7, built from three α-helices α1 orange), linked by 6 disulfide bridges (colored red).N and C correspond to the N-and ends of the polypeptide chain, respectively.(B) Molecular surface of Pru p 7. (C) Elec tentials displayed on the molecular surface of Pru p 7 (electronegative and electropos are colored red and blue, respectively, and neutral regions are colored grey).

Figure 20 .
Figure 20.(A) Ribbon diagram of the modelled Pru p 7, built from three α-helices α1-α3 (colored orange), linked by 6 disulfide bridges (colored red).N and C correspond to the N-and C-terminal ends of the polypeptide chain, respectively.(B) Molecular surface of Pru p 7. (C) Electrostatic potentials displayed on the molecular surface of Pru p 7 (electronegative and electropositive regions are colored red and blue, respectively, and neutral regions are colored grey).

Figure 21 .
Figure 21.(A) Bottom view of the ribbon diagram of the modelled Pru p 7, showing the of the predicted IgE-binding epitopic regions 1 (colored red), 2 (colored blue), and 3 (colo N and C indicate the N-and C-termini of the polypeptide chain of Pru p 7. (B) Bottom molecular surface of Pru p 7 showing the extent of the predicted IgE-binding epitopic re patch), 2 (blue patch), and 3 (green patch).(C).Bottom view of the molecular surface showing the distribution of the electronegative (colored red) and electropositive (color eas.Neutral areas are colored white.

Figure 21 .
Figure 21.(A) Bottom view of the ribbon diagram of the modelled Pru p 7, showing the localization of the predicted IgE-binding epitopic regions 1 (colored red), 2 (colored blue), and 3 (colored green).N and C indicate the N-and C-termini of the polypeptide chain of Pru p 7. (B) Bottom view of the molecular surface of Pru p 7 showing the extent of the predicted IgE-binding epitopic regions 1 (red patch), 2 (blue patch), and 3 (green patch).(C).Bottom view of the molecular surface of Pru p 7 showing the distribution of the electronegative (colored red) and electropositive (colored blue) areas.Neutral areas are colored white.

Figure 24 .
Figure 24.(A) Ribbon diagram of Ara h 13 built from three β-strands (β1-β3), linked to a single αhelix (α).N and C correspond to the N-and C-terminal ends of the polypeptide chain, respectively (B) Molecular surface of Ara h 13. (C) Electrostatic potentials displayed on the molecular surface of Ara h 13 (electronegative and electropositive regions are colored red and blue, respectively, and neutral regions are colored grey).

Figure 24 .
Figure 24.(A) Ribbon diagram of Ara h 13 built from three β-strands (β1-β3), linked to a single α-helix (α).N and C correspond to the N-and C-terminal ends of the polypeptide chain, respectively.(B) Molecular surface of Ara h 13. (C) Electrostatic potentials displayed on the molecular surface of Ara h 13 (electronegative and electropositive regions are colored red and blue, respectively, and neutral regions are colored grey).

Figure 24 .
Figure 24.(A) Ribbon diagram of Ara h 13 built from three β-strands ( helix (α).N and C correspond to the N-and C-terminal ends of the poly (B) Molecular surface of Ara h 13. (C) Electrostatic potentials displayed Ara h 13 (electronegative and electropositive regions are colored red neutral regions are colored grey).

Figure 25 .
Figure 25.Ribbon diagram of Art v 1 (A) and modelled Amb a 4 (B) a the defensin-fold domain and the proline-rich domain are colored vio The four disulfide bonds responsible for the knottin-fold of the defen red.N and C indicate the N-and C-terminal ends of the polypeptide ch

Figure 25 .
Figure 25.Ribbon diagram of Art v 1 (A) and modelled Amb a 4 (B) allergens.In both structures, the defensin-fold domain and the proline-rich domain are colored violet and green, respectively.The four disulfide bonds responsible for the knottin-fold of the defensin-like proteins are colored red.N and C indicate the N-and C-terminal ends of the polypeptide chains of Art v 1 and Amb a 4.

Figure 26 .
Figure 26.Ribbon diagram (A) and molecular surface (B) of Art v 1 epitopic amino acid residues participating in the binding of IgE.

Figure 26 .
Figure 26.Ribbon diagram (A) and molecular surface (B) of Art v 1, showing the localization of epitopic amino acid residues participating in the binding of IgE.

Figure 26 .
Figure 26.Ribbon diagram (A) and molecular surface (B) of Art v 1, showing the localizatio epitopic amino acid residues participating in the binding of IgE.

Figure 28 .
Figure 28.Unrooted phylogenetic tree built from the amino acid sequence alignment of defe allergens from fruits (brazzein from Pentadiplandra brazzeana, Capsicum annuum Cap a DEF, Ma indica Man I DEF, Persea americana Per a DEF, Petunia integrifolia Pet i DEF, Prunus persica DEF, P pyrifolia Pyr py DEF, Solanum lycopersicum Sol l DEF), seeds (Arachis duranensis Ara d DEF, Ar hypogaea Ara h 12 and Ara h 13, Brassica napus Bra na DEF, Cicer arietinum Cic a DEF, Helia annuus Hel a DEF, Lens culinaris Len c DEF, Phaseolus vulgaris Pha v DEF, Raphanus sativus Ra DEF, Ricinus communis DEF, Sinapis alba Sin a DEF, Triticum aestivum Tri a DEF, Vigna radiata DEF), other vegetative parts (Apium graveolens Api g 7, Avena sativa Ave s DEF, Citrus sinensis DEF, Cucumis sativus Cuc sa DEF, Cucurbita maxima Cuc ma DEF, Malus domestica Mal d DEF, europaea Ole e DEF, Pachyrhizus erosus Pac e DEF, Saccharum officinarum Sac o DEF, Solanum tuber Sol t DEF), and pollen (Ambrosia artemisiifolia Amb a 4, Artemisia vulgaris Art v 1, Parthenium h ophorus DEF) of higher plants.GRP allergens of fruits, seeds and pollen are highlighted in red low, and green, respectively.GRP allergens located in vegetative organs of plants are in bold le For clarity, many other pollen defensin allergens listed in the official WHO/IUIS database hav been included in the tree (Art an 7, Art ar 7, Art c 7, Art gm 7, Art la 7 and Art si 7 from Arte annua, A. argyi, A. capillaris, A. gmelinii, A. lavandulifolia and A. sieversiana, respectively).
Plant chitinases essentially consist of endo chitinases that are classically divided five classes, including class-I, class-II, class-III, class-IV, and class-V [55]: -Class-I and class-IV chitinases exhibit a structural organization in two domains, c prising a N-terminal chitin-binding domain made of a cysteine-rich polypeptide, ilarly folded as the hevein domain of the latex from rubber tree Hevea brasiliensis ( b 6), linked by an extended linker loop to a C-terminal α-helical folded domain a chitin-cleavage activity (Figure 29A).Class-I and class-IV chitinases use an in ing mechanism for cleaving the β1,4-GlcNAc linkage of chitin to generate sho fragments of chitobiose (GlcNAc)2, chitotriose (GlcNAc)3 and chitotetraose ( NAc)4.The chitinase allergens from banana (Mus a 2), avocado (Pers a 1), and H 11 from the latex of rubber tree, belong to this group of chitinases.-Class-III chitinases readily differ from class-I chitinases by a different α8b8 β-b organization made of a central crown of eight β-strands, linked to a peripheral cr of eight α-helix by interconnecting loops (Figure 29B) [56].In addition, class-III tinases use a different retaining mechanism for the cleavage of chitin chains in sho fragments.The chitinase allergens from pomegranate (Pun g 14) and jujuba fruit m 1), belong to the class-III chitinase group.

Figure 28 .Figure 29 .Figure 29 .
Figure 28.Unrooted phylogenetic tree built from the amino acid sequence alignment of defensin allergens from fruits (brazzein from Pentadiplandra brazzeana, Capsicum annuum Cap a DEF, Magifera indica Man I DEF, Persea americana Per a DEF, Petunia integrifolia Pet i DEF, Prunus persica DEF, Pyrus pyrifolia Pyr py DEF, Solanum lycopersicum Sol l DEF), seeds (Arachis duranensis Ara d DEF, Arachis hypogaea Ara h 12 and Ara h 13, Brassica napus Bra na DEF, Cicer arietinum Cic a DEF, Helianthus annuus Hel a DEF, Lens culinaris Len c DEF, Phaseolus vulgaris Pha v DEF, Raphanus sativus Raph s DEF, Ricinus communis DEF, Sinapis alba Sin a DEF, Triticum aestivum Tri a DEF, Vigna radiata Vig r DEF), other vegetative parts (Apium graveolens Api g 7, Avena sativa Ave s DEF, Citrus sinensis Cit si DEF, Cucumis sativus Cuc sa DEF, Cucurbita maxima Cuc ma DEF, Malus domestica Mal d DEF, Olea europaea Ole e DEF, Pachyrhizus erosus Pac e DEF, Saccharum officinarum Sac o DEF, Solanum tuberosum Sol t DEF), and pollen (Ambrosia artemisiifolia Amb a 4, Artemisia vulgaris Art v 1, Parthenium hysterophorus DEF) of higher plants.GRP allergens of fruits, seeds and pollen are highlighted in red, yellow, and green, respectively.GRP allergens located in vegetative organs of plants are in bold letters.For clarity, many other pollen defensin allergens listed in the official WHO/IUIS database have not been included in the tree (Art an 7, Art ar 7, Art c 7, Art gm 7, Art la 7 and Art si 7 from Artemisia annua, A. argyi, A. capillaris, A. gmelinii, A. lavandulifolia and A. sieversiana, respectively).2.7.PR3 Chitinases Plant chitinases essentially consist of endo chitinases that are classically divided into five classes, including class-I, class-II, class-III, class-IV, and class-V [55]: -Class-I and class-IV chitinases exhibit a structural organization in two domains, comprising a N-terminal chitin-binding domain made of a cysteine-rich polypeptide, similarly folded as the hevein domain of the latex from rubber tree Hevea brasiliensis (Hev b 6), linked by an extended linker loop to a C-terminal α-helical folded domain with a chitin-cleavage activity (Figure 29a).Class-I and class-IV chitinases use an inverting mechanism for cleaving the β1,4-GlcNAc linkage of chitin to generate shorter fragments of chitobiose (GlcNAc) 2 , chitotriose (GlcNAc) 3 and chitotetraose (GlcNAc) 4 .The chitinase allergens from banana (Mus a 2), avocado (Pers a 1), and Hev b 11 from the latex of rubber tree, belong to this group of chitinases.-Class-III chitinases readily differ from class-I chitinases by a different α 8 β 8 β-barrel organization made of a central crown of eight β-strands, linked to a peripheral crown of eight α-helix by interconnecting loops (Figure 29b) [56].In addition, class-III chitinases use a different retaining mechanism for the cleavage of chitin chains in shorter fragments.The chitinase allergens from pomegranate (Pun g 14) and jujuba fruit (Ziz m 1), belong to the class-III chitinase group.

Figure 30 .Figure 31 .
Figure 30.(A) Ribbon diagram and molecular surface shown in transparency of hevein (Hev b 6).(B) Localization of IgE-binding epitopic regions (colored cyan and violet) on the molecular surface of hevein.(C) Localization of epitopes T (colored magenta and green) on the molecular surface of hevein.The alignments of PR3 chitinase allergens from higher plants clearly discriminate between class-I/class-IV and class-III chitinase (Pun g 14, Ziz m 1) groups (Figure31).However, class-I and class-IV chitinases exhibit rather different amino acid sequences, particularly in their N-terminal part.

Figure 33 .
Figure 33.A,B.Cartoons showing the molecular organization in α8β8 β-barrel structure of Mus a 5 (PDB code 2CYG) (A), and Hev b 2 (PDB code 4HPG) (B).The catalytic groove responsible for the enzymatic activity is indicated by a black dashed line on the front face of the molecules.N and C correspond to the N-and C-termini of the polypeptide chains of Mus a 5 and Hev b 2, respectively

Figure 33 .
Figure 33.A,B.Cartoons showing the molecular organization in α8β8 β-barrel structure of Mus a 5 (PDB code 2CYG) (A), and Hev b 2 (PDB code 4HPG) (B).The catalytic groove responsible for the enzymatic activity is indicated by a black dashed line on the front face of the molecules.N and C correspond to the N-and C-termini of the polypeptide chains of Mus a 5 and Hev b 2, respectively

Figure 33 .
Figure 33.(A,B) Cartoons showing the molecular organization in α 8 β 8 β-barrel structure of Mus a 5 (PDB code 2CYG) (A), and Hev b 2 (PDB code 4HPG) (B).The catalytic groove responsible for the enzymatic activity is indicated by a black dashed line on the front face of the molecules.N and C correspond to the N-and C-termini of the polypeptide chains of Mus a 5 and Hev b 2, respectively.

Figure 37 .
Figure 37. (A) Ribbon diagram structure of the modelled homohexameric allergen Zan b chuan pepper (Xanthoxylum bungeanum) 11S globulin, made of two superimposed trimers mers with a cupin fold.(B) Molecular surface of Zan b 2. (C) Electrostatic potentials dis the molecular surface of Zan b 2 (electronegatively and electropositively charged regions a red and blue, respectively, and neutral regions are colored grey).

Figure 38 .
Figure 38.Distribution of four continuous IgE-binding epitopic regions 1 (colored red), orange), 3 (colored cyan), and 4 (colored magenta), shared in common by the 11S globulin from fruits and seeds, on the molecular surface of the homodimeric structures of Act d 1 Fra v-Leg (B).

Figure 37 .
Figure 37. (A) Ribbon diagram structure of the modelled homohexameric allergen Zan b 2, the Sichuan pepper (Xanthoxylum bungeanum) 11S globulin, made of two superimposed trimers of monomers with a cupin fold.(B) Molecular surface of Zan b 2. (C) Electrostatic potentials displayed on the molecular surface of Zan b 2 (electronegatively and electropositively charged regions are colored red and blue, respectively, and neutral regions are colored grey).

Figure 37 .
Figure 37. (A) Ribbon diagram structure of the modelled homohexameric allergen Zan b chuan pepper (Xanthoxylum bungeanum) 11S globulin, made of two superimposed trimers mers with a cupin fold.(B) Molecular surface of Zan b 2. (C) Electrostatic potentials dis the molecular surface of Zan b 2 (electronegatively and electropositively charged regions a red and blue, respectively, and neutral regions are colored grey).

Figure 38 .
Figure 38.Distribution of four continuous IgE-binding epitopic regions 1 (colored red), orange), 3 (colored cyan), and 4 (colored magenta), shared in common by the 11S globulin from fruits and seeds, on the molecular surface of the homodimeric structures of Act d 1 Fra v-Leg (B).

Figure 38 .
Figure 38.Distribution of four continuous IgE-binding epitopic regions 1 (colored red), 2 (colored orange), 3 (colored cyan), and 4 (colored magenta), shared in common by the 11S globulin allergens from fruits and seeds, on the molecular surface of the homodimeric structures of Act d 12 (A) and Fra v-Leg (B).

Figure 41 .
Figure 41.(A) Ribbon diagram structure of the modelled germin-like protein Cit s 1 from orange fruit, made of a trimer of dimers with a cupin fold.(B) Molecular surface of Cit s 1. (C) Electrostatic potentials displayed on the molecular surface of Cit s 1 (electronegative and electropositive regions are colored red and blue, respectively, and neutral regions are colored grey).

Figure 42 .
Figure 42.Extended N-glycan chains of the complex type (colored cyan), associated with the front face (A) and back face (B) of Cit s 1. N-glycans of the complex type were modelled with the GlyProt server.

Figure 41 .
Figure 41.(A) Ribbon diagram structure of the modelled germin-like protein Cit s 1 from orange fruit, made of a trimer of dimers with a cupin fold.(B) Molecular surface of Cit s 1. (C) Electrostatic potentials displayed on the molecular surface of Cit s 1 (electronegative and electropositive regions are colored red and blue, respectively, and neutral regions are colored grey).

Figure 41 .
Figure 41.(A) Ribbon diagram structure of the modelled germin-like protein Cit s 1 from fruit, made of a trimer of dimers with a cupin fold.(B) Molecular surface of Cit s 1. (C) Elec potentials displayed on the molecular surface of Cit s 1 (electronegative and electropositive are colored red and blue, respectively, and neutral regions are colored grey).

Figure 42 .
Figure 42.Extended N-glycan chains of the complex type (colored cyan), associated with t face (A) and back face (B) of Cit s 1. N-glycans of the complex type were modelled with the server.

Figure 42 .
Figure 42.Extended N-glycan chains of the complex type (colored cyan), associated with the front face (A) and back face (B) of Cit s 1. N-glycans of the complex type were modelled with the GlyProt server.

Figure 44 .
Figure 44.Unrooted phylogenetic tree built from the amino acid sequence alignment of germin-like protein allergens from fruits (Capsicum annuum Cap a GERM, Citrus sinensis Cit s 1, Malus domestica Mal d GERM, Morus alba Mor a GERM, Musa acuminata Mus a GERM, Prunus persica Pru p GERM, Solanum lycopersicum Sol l GERM, Vitis vinifera Vit v GERM) and seeds (Arachis hypogaea Ara h 3 GERM, Cicer arietinum Cic a GERM, Glycine max Gly m GERM, Phaseolus vulgaris Pha v GERM, Pisum sativum Pis s GERM, Theobroma cacao The c GERM, Vigna radiata Vig r GERM).Germin allergens of fruits and seeds are highlighted in red and yellow, respectively.

Figure 45 .
Figure 45.Cartoon showing the diversity of potential allergens of pericarp and seeds from toma fruit (Solanum lycopersicum).Allergens listed in the official WHO/IUIS database are highlighted red.Other tomato allergens were retrieved from Allergome (https://www.Allergome.org),assess on 16 April 2023, and either modelled or retrieved from the PDB (https://www.rcsb.org),assess on 16 April 2023.

Figure 45 .
Figure 45.Cartoon showing the diversity of potential allergens of pericarp and seeds from tomato fruit (Solanum lycopersicum).Allergens listed in the official WHO/IUIS database are highlighted in red.Other tomato allergens were retrieved from Allergome (https://www.Allergome.org),accessed on 16 April 2023, and either modelled or retrieved from the PDB (https://www.rcsb.org),accessed on 16 April 2023.