Shoot Dieback in Thornless Blackberries in Northern Spain Caused by Diaporthe rudis and Gnomoniopsis idaeicola

: A cane disease of a non-commercial thornless blackberry cultivar (genus Rubus , subgenus Rubus Watson) obtained in a breeding program was observed in May 2021 in northern Spain during a ﬁeld evaluation. Symptoms of the disease appeared in spring and ﬁrstly consisted of dark-brown lesions in the petioles, tips, and intermediate zones of the canes, ﬁnally causing the leaves, canes, and lateral shoots to die. Two strains were recovered from infected canes and identiﬁed by morphological characteristics and multigene analysis as Gnomoniopsis idaeicola (LPPAF-977) and Diaporthe rudis (LPPAF-981). Pathogenicity tests showed that both fungi caused shoot dieback when artiﬁcially inoculated, reproducing the symptoms originally observed. Moreover, tissue necrosis was enhanced when Diaporthe rudis and Gnomoniopsis idaeicola were co-inoculated. This is the ﬁrst report of Diaporthe rudis and Gnomoniopsis idaeicola causing a potentially serious disease to blackberries in Spain.


Introduction
Blackberries, overall considered in the genus Rubus L., subgenus Rubus Watson, by most authorities [1], are a taxonomically complex group of perennial and vigorous plants belonging to the family Rosaceae. Blackberries are distributed in all continents, except in Antarctica. Taking advantage of the interfertility, heteroploidy, and high heterogeneity of the wild species that easily forms natural hybrids, domestication from nature's breeding programs was performed by the seventeenth century in Europe and during the nineteenth century in North America [2]. Mainly, thanks to the superior cultivars obtained by the mid-nineteenth century, interest in blackberry cultivation has increased and encouraged private and public breeding programs, resulting in hundreds of cultivars to date. Many of them have erect growth habit and, since the isolation in the 1980s of 'Navaho', the first erect thornless cultivar, this class has become usual due to its high productive and improved fruit-quality characteristics [1]. Most species of blackberries typically bear biennial stems named canes from the perennial root system. During the first year, canes grow and usually do not flower and are named primocanes. After a winter dormant period, primocanes flower and fruit, and then are termed floricanes. In addition, a new type of erect blackberry named primocane-fruiting was achieved for the first time in 2004 [3], being able to produce flowers and fruit on the primocane, in addition to the floricane.
One the one hand, in recent decades, we have seen an increasing interest in the health benefits of fruits and vegetables, as consumers are not only looking for nutritional properties in foods, but also for these to be natural and healthy. Similar to most blue, red, or black vegetables, one of the most outstanding characteristics of blackberries is their high content in antioxidant compounds, such as natural pigments, vitamins, and polyphenols, which are highly valued for their health benefits [4][5][6][7]. This trend contributes to the higher demand for berries, including blackberries, whereby the global, fresh berry market is projected to register a compound annual growth rate (CAGR) of 2.5% during the forecasted period (2021-2026) [8].
As far as the genus Diaporthe Fuckel 1867 (synonym Diaporthe Nitschke 1870) (Diaporthales, Diaporthaceae) is concerned, it includes both saprophytic and pathogenic species of a large number of plants. Diaporthe represents a highly complex genus containing numerous confusing species, the type species being D. alnea Fuckel 1867. A more in-depth study of the status of the taxonomy in this genus has been conducted by several authors [14][15][16][17]. Symptoms produced are root and fruit rot, dieback, stem cankers, leaf spots, leaf and pod blight, and seed decay [18].
The genus Gnomoniopsis Berl. 1893 (Diaporthales, Gnomoniaceae), whose type species is G. chamaemori (Fr.) Berl. Barr 1978, is considered to be a synonym of Gnomonia (Barr 1978, Monod 1983, and, at present, contains species that include several pathogens that cause diseases in plants of considerable economic importance belonging mainly to the families Rosaceae, Fagaceae, and Tiliaceae [19].
Regarding Diaporthe, several studies have shown that many species are associated with numerous hosts around the world, which highlights its high degree of dissemination and distribution, e.g., D. eres can infect species of economic interest, such as blackberry in Croatia [10], peach trees in Greece [20], and pear and jujube in China [21,22].
In this context, cane wilt was observed for the first time in thornless blackberry plants in a field evaluation in Villaviciosa, Asturias, Spain (43°28′31.58″ N 5°26′34.59″ W, 5 m asl), in May 2021. On the other hand, the plants to be evaluated, obtained from a breeding program, were provided by a private nursery located in Central Spain. Several petioles, shoots, and canes of each plant showed visible necrotic symptoms ( Figure 1) and it was observed that shoot injury progressed from the shoot tip to the base, causing dieback. The main objective of this study is to identify the causal agent/s of the said symptoms in blackberry plants.

Sample Analysis
Samples recovered from symptomatic blackberry canes were surface sterilized with 96% ethanol for 30 s, dried on sterilized tissue paper, plated on potato dextrose agar (PDA, Difco, France), and incubated at 25 °C for 2-5 days.

Sample Analysis
Samples recovered from symptomatic blackberry canes were surface sterilized with 96% ethanol for 30 s, dried on sterilized tissue paper, plated on potato dextrose agar (PDA, Difco, France), and incubated at 25 • C for 2-5 days.

Identification
The identification of the two fungal strains was initially performed by the observation of their morphological characteristics and further confirmed by multigene analysis, including ITS (internal transcribed spacer), tub2 (β-tubulin), and tef-1a (translation elongation factor) genes. In the case of Diaporthe, calmodulin (cal) and histone (his) were also included. The genes were amplified using the Terra TM PCR Direct Polymerase Mix (Takara Bio Company, CA, USA). The primers and PCR conditions were those described in the references included in Table 1. All sequences obtained in this work were deposited in GenBank (Supplementary Tables S1 and S2). A comparison of the sequences obtained with those deposited in data banks was performed by BLAST.

Multigen Analysis
Multiple sequence alignments were performed using Clustal W [28]. Phylogenetic trees were constructed using the maximum likelihood method based on the Tamura-Nei model [29], and their topological robustness was evaluated by bootstrap analysis based on 1000 replicates using Mega version 11 software [30].
The sequences available in the databases of the species corresponding to the genera Gnomoniopsis and Diaporthe (Supplementary Tables S1 and S2, respectively) were used to obtain the phylogenetic trees. Sirococcus castaneae and Diaporthella corylina were included as outgroups for Gnomoniopsis and Diaporthe, respectively.
Nucleotide diversity and the number of recombinant sites were calculated using the Tajima test [31].

Pathogenicity Tests
The tests were conducted on 18-month-old blackberry plants acquired from a commercial nursery in northern Spain specializing in small fruits. Plants were grown in 2.8 L containers in a greenhouse and provided with drip irrigation. Each plant was watered daily with an average of 0.35 L for the duration of the study. An isolate of each of the two fungi derived from the blackberry plants was used in the pathogenicity tests. Each of the two isolates, as well as the combination of both, was inoculated into a lateral shoot on 10 blackberry plants. Half of a 5 mm diameter PDA disc with actively growing fungus was inserted into the end of a shoot by making a longitudinal cut at the end, its tip being previously blunted, and covered with Parafilm© to prevent it from falling off or drying out. Co-inoculation with both fungi was conducted by inserting two quarters of a PDA disc containing each of the two fungi to be inoculated in the artificial cut. Ten blackberry plants were also inoculated with PDA plugs without fungi to use as a negative control.
A randomized block design with two replicates for the inoculations with each of the treatments (LPPAF-977, LPPAF-981, co-inoculation with the two strains, fungus-free control) was established. Co-inoculation was performed by inserting one strain first, followed by the second one in a replicate and in reverse order in the other replicate. Each replicate consisted The sampled shoots were cut 25 days after the inoculation and taken to the laboratory. For each treatment, the number of shoots with canker symptoms was registered and the percentage of shoots with damage was calculated; the length of the vertical lesion on symptomatic shoots was also recorded using a 0-150 mm digital vernier electronic caliper.
With the aim of recovering the strains, the shoots were processed in the same way as said for the symptomatic samples collected in the experimental planting.
To ascertain if the fungi were synergistic, an in vitro test was used. Both fungi were cultured at the same time on a PDA plate with only 2 mm spacing between them and incubated for 5 days at 25 • C. Five replicates were created.

Statistical Analysis
The data were subjected to the relevant statistical analysis using SPSS ® Win TM , version 12.0. (SPSS Inc., Chicago, IL, USA). An analysis of variance (ANOVA) was conducted to detect the effects of the pathogen treatments on the vertical lesion, followed by Tukey's multiple comparison test to check significance at the 5% probability level, and a chi-squared analysis was performed for the qualitative variable presence of canker shoots.

Pathogen Isolation
Two fungi were consistently isolated from the analyzed tissue samples and the respective strains selected were named LPPAF-977 and LPPAF-981.

Morphological Characteristics
The strain LPPA-977 grew, developing white circular colonies on the agar surface with sparse to dense aerial hyphae. Conidia were single-celled, hyaline, consistent to Gnomoniopsis, whereas strain LPPA-981 showed a white or gray mycelium with α and β conidia compatible with the genus Diaporthe ( Figure 2). cultured at the same time on a PDA plate with only 2 m incubated for 5 days at 25 °C. Five replicates were created.

Statistical Analysis
The data were subjected to the relevant statistical analy 12.0. (SPSS Inc., Chicago, IL, USA). An analysis of varianc detect the effects of the pathogen treatments on the vertic multiple comparison test to check significance at the 5% squared analysis was performed for the qualitative variab

Pathogen Isolation
Two fungi were consistently isolated from the analy spective strains selected were named LPPAF-977 and LPP

Morphological Characteristics
The strain LPPA-977 grew, developing white circula with sparse to dense aerial hyphae. Conidia were single-ce moniopsis, whereas strain LPPA-981 showed a white or gr nidia compatible with the genus Diaporthe ( Figure 2).

Identification
Strain LPPAF-977 by BLAST analysis showed that the sion no. OQ933371) had a 99.48% similarity with the acces age) isolated from Rubus fruticosus in New Zealand. The tub no. OQ991913) presented 99.38% identity with accession isolated from Rubus fruticosus in Serbia, and the tef-1a seq OQ991914) showed 99.38% identity with accession no. MG

Identification
Strain LPPAF-977 by BLAST analysis showed that the ITS sequence of 584 bp (accession no. OQ933371) had a 99.48% similarity with the accession no. KC145872 (99% coverage) isolated from Rubus fruticosus in New Zealand. The tub2 sequence of 503 bp (accession no. OQ991913) presented 99.38% identity with accession no. MG860503 (94% coverage) isolated from Rubus fruticosus in Serbia, and the tef-1a sequence of 325 bp (accession no. OQ991914) showed 99.38% identity with accession no. MG773589 (99% coverage) isolated from R. fruticosus in Serbia, all of them corresponding to Gnomoniopsis idaeicola.

Multigen Analysis
For Gnomoniopsis, phylogenetic trees were constructed with the single partial sequences of ITS, tub2, and tef-1a genes, and with the three concatenated sequences using those listed in Supplementary Table S1. In the phylogenetic tree obtained with each of the three genes separately, strain LPPAF-977 always clustered with G. idaeicola. The phylogenetic tree with the concatenated sequences of the three loci (total length of 1163 bp (461 bp ITS, 420 bp tub2, and 282 bp tef-1a)) is shown in Figure 3, where we can see that our strain clusters with G. idaeicola with a 100% bootstrap.
With LPPA-981, in the phylogenetic tree created with the single partial sequences of ITS, tub2, tef-1a, and his genes, this strain clustered with D. rudis, while with the cal gene, it clustered with D. canthi (86%) species, far removed from the result obtained for the rest of the genes. Thus, cal was not used in the phylogenetic tree with concatenated sequences. The accession numbers of sequences used are listed in Supplementary Table S2.
The phylogenetic tree with the four concatenated genes showed that LPPAF-981 clustered with D. rudis with a 100% bootstrap (Figure 4).
By means of the Tajima's test of neutrality, we were able to identify the number of segregating sites and the nucleotide diversity (π) for LPPAF-977 and LPPAF-981 (Table 2).
In both strains, tefl-1a provided the highest nucleotide diversity, whereas, as expected, ITS had the lowest diversity. This may indicate that while ITS is a good primary marker for taxonomy, it may be better not to include it in phylogenetic trees.

Pathogenicity
Symptoms observed in the field were reproduced on inoculated shoots 25 days after the inoculation ( Figure 5). The effects of the fungi treatments in relation to the shoots of blackberries are shown in Figure 6. The results obtained demonstrate significant differences (p < 0.001) between pathogen treatments in terms of the average percentage (%) of canker shoots and vertical lesion (mm). those listed in Supplementary Table S1. In the phylogenetic tree obtained with each of the three genes separately, strain LPPAF-977 always clustered with G. idaeicola. The phylogenetic tree with the concatenated sequences of the three loci (total length of 1163 bp (461 bp ITS, 420 bp tub2, and 282 bp tef-1a)) is shown in Figure 3, where we can see that our strain clusters with G. idaeicola with a 100% bootstrap. With regard to the percentage of canker shoots, 100% of the blackberry shoots inoculated with LPPAF-977 as well as those inoculated with the two fungi showed dark-brown lesions, while 10% of the inoculated ones with LPPAF-981 showed no wilt symptoms ( Figure 5). Significantly longer tissue necrosis was observed in blackberry shoots coinoculated with the two pathogens, with a mean lesion length of 40.79 mm. In contrast, shoots inoculated with LPPAF-981 significantly had the lowest values for the lesion lengths: 26.17 mm. No significant differences in the vertical lesion between two pathogens were observed when inoculated independently.
As expected, no symptoms were observed for the two variables analyzed, nor were the fungi under study recovered in any of the control plants, while they were recovered in 100% of the symptomatic tissues of the inoculated blackberry plants, thus fulfilling Koch's postulates.
When the two strains were sown together in a plate with PDA medium, it was possible to verify that LPPAF-981 grew faster and surrounded LPPAF-977 in the five replicates conducted (Figure 7).    With regard to the percentage of canker shoots, 100% of the blackberry shoots inoculated with LPPAF-977 as well as those inoculated with the two fungi showed darkbrown lesions, while 10% of the inoculated ones with LPPAF-981 showed no wilt symptoms ( Figure 5). Significantly longer tissue necrosis was observed in blackberry shoots coinoculated with the two pathogens, with a mean lesion length of 40.79 mm. In contrast, shoots inoculated with LPPAF-981 significantly had the lowest values for the lesion lengths: 26.17 mm. No significant differences in the vertical lesion between two pathogens were observed when inoculated independently. With regard to the percentage of canker shoots, 100% of the blackberry shoots inoculated with LPPAF-977 as well as those inoculated with the two fungi showed darkbrown lesions, while 10% of the inoculated ones with LPPAF-981 showed no wilt symptoms ( Figure 5). Significantly longer tissue necrosis was observed in blackberry shoots coinoculated with the two pathogens, with a mean lesion length of 40.79 mm. In contrast, shoots inoculated with LPPAF-981 significantly had the lowest values for the lesion lengths: 26.17 mm. No significant differences in the vertical lesion between two pathogens were observed when inoculated independently. As expected, no symptoms were observed for the two variables analyzed, nor were the fungi under study recovered in any of the control plants, while they were recovered in 100% of the symptomatic tissues of the inoculated blackberry plants, thus fulfilling Koch's postulates.
When the two strains were sown together in a plate with PDA medium, it was possible to verify that LPPAF-981 grew faster and surrounded LPPAF-977 in the five replicates conducted (Figure 7).

Discussion
This study reported for the first time the pathogenicity of two fungi, Gnomoniopsis idaeicola and Diaporthe rudis, causing shoot dieback on blackberries in Spain.
In this work, the identification of the LPPAF-977 strain as G. idaeicola was successfully achieved thanks to the BLAST analysis and individual and concatenated phylogenetic trees. This result is in agreement with other authors using the same three genes (ITS, tub2, and tef-1a) employed in this work [17,32]. It is worth noting that in the Index Fungorum (accessed 8 November 2022) [33], there are 41 species listed in the Gn. Gnomoniopsis; however, nine have been included within the Plagiostoma and Colletotrichum genera. Therefore, As expected, no symptoms were observed for the two variables analyzed, nor were the fungi under study recovered in any of the control plants, while they were recovered in 100% of the symptomatic tissues of the inoculated blackberry plants, thus fulfilling Koch's postulates.
When the two strains were sown together in a plate with PDA medium, it was possible to verify that LPPAF-981 grew faster and surrounded LPPAF-977 in the five replicates conducted (Figure 7).

Discussion
This study reported for the first time the pathogenicity of two fungi, Gnomoniopsis idaeicola and Diaporthe rudis, causing shoot dieback on blackberries in Spain.
In this work, the identification of the LPPAF-977 strain as G. idaeicola was successfully achieved thanks to the BLAST analysis and individual and concatenated phylogenetic trees. This result is in agreement with other authors using the same three genes (ITS, tub2, and tef-1a) employed in this work [17,32]. It is worth noting that in the Index Fungorum (accessed 8 November 2022) [33], there are 41 species listed in the Gn. Gnomoniopsis; however, nine have been included within the Plagiostoma and Colletotrichum genera. Therefore, ). Chi-squared analysis is shown for percentage data (***: p < 0.001) (A). Different capital letters indicate significant differences (p < 0.001) among pathogens, according to a posteriori Tukey's test (B). Figure 6. Results of field pathogenicity tests comparing the percentage o length of the necrotic vertical lesion (mm) (B) between blackberry cane without pathogens. Boxplots indicate 25-75% quartiles, median (horiz (whiskers), mean values (crosses), and extreme values of the length of analysis is shown for percentage data (***: p < 0.001) (A). Different capita differences (p < 0.001) among pathogens, according to a posteriori Tuke As expected, no symptoms were observed for the two varia the fungi under study recovered in any of the control plants, wh in 100% of the symptomatic tissues of the inoculated blackber Koch's postulates.
When the two strains were sown together in a plate with P sible to verify that LPPAF-981 grew faster and surrounded LPP cates conducted (Figure 7).

Discussion
This study reported for the first time the pathogenicity of idaeicola and Diaporthe rudis, causing shoot dieback on blackberri In this work, the identification of the LPPAF-977 strain as G. achieved thanks to the BLAST analysis and individual and co trees. This result is in agreement with other authors using the sam and tef-1a) employed in this work [17,32]. It is worth noting tha (accessed 8 November 2022) [33], there are 41 species listed in the ever, nine have been included within the Plagiostoma and Colletotr

Discussion
This study reported for the first time the pathogenicity of two fungi, Gnomoniopsis idaeicola and Diaporthe rudis, causing shoot dieback on blackberries in Spain.
In this work, the identification of the LPPAF-977 strain as G. idaeicola was successfully achieved thanks to the BLAST analysis and individual and concatenated phylogenetic trees. This result is in agreement with other authors using the same three genes (ITS, tub2, and tef-1a) employed in this work [17,32]. It is worth noting that in the Index Fungorum (accessed 8 November 2022) [33], there are 41 species listed in the Gn. Gnomoniopsis; however, nine have been included within the Plagiostoma and Colletotrichum genera. Therefore, these strains were excluded from this study. G. castaneae is synonymous with G. smithogilvyi; therefore, only one of them was included in the phylogenetic tree.
Identification by BLAST of LPPAF-981 as D. rudis was successful with the five genes sequenced (ITS, tub2, tef-1a, his, and cal). In contrast, it was more complex when construct-ing the phylogenetic trees. Despite the fact that Guarnaccia et al. [34] used only three genes (ITS, tub2, and tef-1a), other authors used up to five loci in previous papers [15,[35][36][37][38]. Santos et al. [39] proposed that tef-1a was a superior phylogenetic marker in Diaporthe compared to ITS, while Gomes et al. [15] considered tub2 and his to have higher resolutions than tef-1a and cal. However, Udayanga et al. [27] suggested that the use of cal may lead to errors in the identification of Diaporthe species due to poor sequence quality because of the low specificity of the primers used. In our study, the results support those of Udayanga et al. [27], because the phylogenetic tree obtained with this gene differs completely from the results obtained with the other four genes used. Therefore, it was discarded for inclusion in the final tree created with the concatenated four sequences; therefore, LPPAF-981 clustered with D. rudis with a 100% bootstrap.
The ITS provided the lowest nucleotide diversity in both species; therefore, it was a first genetic marker, but was better for identification to use other genes, such as tef-1a, which was the gene that provided the highest nucleotide diversity that matched the results of Santos et al. [39]. The second gene that provided the highest nucleotide diversity was bet-2 that partially matched with the results of Gomes et al. [15]. Thus, it would not be essential to include the ITS sequence in the phylogenetic tree because it did not provide much differential information. In addition, the use of ITS made it difficult to correctly apply the software used to build phylogenetic trees because it differentiated between the sequences that coded for proteins and those that did not.
G. idaeicola caused noticeable damage as a pathogen in Rubus. Mirhosseini et al. [11] estimated a 30% loss of extremely symptomatic blackberry plants and yield losses of 50-80% in Iran. Stevanović et al. [12] described G. idaeicola as being responsible for the canker, wilt, and death of blackberries in Serbia, reaching an incidence of up to 80%, being responsible for up to 40% plant mortality and estimated yield losses of 50%. These authors also found this species in mixed infections with other fungi, among which was Diaporthe. In Oregon [13], this fungus caused 17% plant mortality. In our study, it was not possible to evaluate the fruit production.
The countries with the highest production of raspberries and blackberries in 2021 were Mexico, Russia, and Serbia, comprising 49% of the production, followed by USA, Poland, Spain, Morocco, Ukraine, Portugal, Bosnia and Herzegovina, the UK, and Chile [8]. Concerning Spain, most blackberry fields are located in southern areas and particularly in the province of Huelva. In northern Spain, where it is also important to consider that the structure of agricultural property is clearly smallholding, blackberry production is on a very small scale. Although local growers are showing great interest in the crop, the production and consumption of blackberries has been gradually increasing, unlike other berries, mainly blueberries and raspberries, which have experienced exponential growth in recent years. Plant breeding programs are aimed at developing crop varieties that are more productive, have a better taste and quality, and are adapted to climate change, pests, and diseases to align with a lower usage of nitrogen fertilizer and pesticides for more sustainable agriculture. The selection of new, high-quality, resistant varieties reduces both the cost of crop production and environmental risks. At present, it is perceived that more consumers are recognizing these aspects as more environmentally friendly. This, coupled with increased health awareness, is contributing to the higher demand for small berries. Our findings suggest the importance of testing new genetics from variety improvement programs for phytosanitary, resilience, and sustainability characteristics well adapted to different climate change scenarios.
In this work, the pathogenic nature of Gnomoniopsis idaeicola and Diaporthe rudis in relation to the shoot dieback of thornless blackberries was confirmed by pathogenicity tests. Nevertheless, both species were co-inoculated twice, each replicate in reverse order, and were also plated together; however, these results do not allow us to establish that there is an antagonism between them. The laboratory results, when both species were sown together, do not correspond with what was observed in the field where G. idaeicola produced a longer vertical lesion than that produced by D. rudis; therefore, this result may indicate that the latter is better adapted to the growth conditions provided by the laboratory. Therefore, further studies must be conducted to clarify this point.
G. idaeicola (P. Karst.) Walker et al. 2010 was described in 1869 as Calosphaeria idaeicola on canes of Rubus idaeus in Finland, and it is present in Finland, France, Bulgaria, Serbia, Spain, Sweden, the USA, Australia, New Zealand, and Iran [40]. This species has been described with a very narrow host range, limited to Rubus spp. [11][12][13]19], but also as endophytes in wheat [41] and Geum peckii [42], and it is present in environmental samples as air from a cave [43] and a sample of human health [44]. There are also records of its presence in Actinidia deliciosa in 1993 in New Zealand (Data of ICMP) and Corylus avellana (data in GenBank), although without information about its pathogenicity.
D. rudis, a synonym for D. viticola [27], has been described as a pathogen in hosts of high agro-economic value, for instance, associated with the stem blight and dieback of blueberries in northern Italy [34], avocados in Chile [45], and Pyrus communis in the USA [46]. In addition, it is also associated with other Diaporthe species causes kiwifruit rot during cold storage in Chile [47] and soybean seed decay in Serbia [48]. It also contributes to grape rot in Italy [49] and was found in Vitis vinifera samples in Portugal, Italy, France, Spain, the UK, and the Czech Republic [36]; however, its pathogenicity has not been tested. Dissanayake et al. [16] described its presence in Cornus sp., Anthoxanthum odoratum, Carlina vulgaris, and Dioscorea communis in Italy. Sequences of strains isolated from many other hosts are available in GenBank, for example, strain ICMO 16419 in Castanea sativa from New Zealand (accession no. KC145904), strain DA244 in Brugmansia sp. from Germany (KC843334), LC6145 in Ilex aquifolium from China, LC6147 Dendrobenthamia japonica from the USA, and CBS 109292 in Laburnum anagyroides from Austria. Thus, since its description, this species has been identified around the world as being associated with numerous hosts [27,50,51], which demonstrates its high degree of dissemination, distribution, and wide host range, similar to that of D. eres.
G. idaeicola has not been previously reported as a pathogen in blackberries in Spain. D. rudis has not been described as a pathogen of Rubus spp. Thus, to the best of our knowledge, this is the first report of G. idaeicola and D. rudis causing dieback in blackberries in Spain.

Supplementary Materials:
The following supporting information can be downloaded at: https://www. mdpi.com/article/10.3390/horticulturae9090965/s1, Table S1: Features of the sequences of Gnomoniopsis species used in this work; Table S2 Funding: This research was funded by the Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA) and the Consejería de Ciencia, Empresas, Formación y Empleo, Gobierno del Principado de Asturias: Own budgets. Data Availability Statement: Sequences obtained in this work have been deposited in GenBank under the accession numbers shown in Supplementary Tables S1 and S2.