Response of Seeds, Oil Yield and Fatty Acids Percentage of Jojoba Shrub Strain EAI to Mycorrhizal Fungi and Moringa Leaves Extract

Jojoba seeds have a unique storage lipid wax which is suitable as a basic feedstock in the chemical industry. For saving both human health and the environment, there is a continuous need to search for alternative safe natural sources of plant nutrients. Therefore, in this study the effect of mycorrhizal fungi and Moringa oleifera leaves extract on growth, flowering, fruits set, yield and the chemical composition of the jojoba shrub was studied. The application of a combination of treatments of 20 g L−1 mycorrhizal fungi plus 30 g L−1Moringa oleifera leaves extract recorded the maximum mean values of main branch length, length of secondary branches, number of branched nodes, number of secondary branches, flowering percentage, final fruit set percentage, seeds yield per shrub and per hectare, percentage of minerals, proteins as well as oil yield per shrub and per hectare, chlorophyll a and b, N, P, K percentage with a minimum mean value of the number of days until full bloom in both seasons. The maximum percentage of Gadoleic fatty acid was found with the combination treatment of uninoculation plus 10 g L−1Moringa oleifera leaves extract.


Introduction
Jojoba (Simmondsia chinensis) is a member of the family Simmondsiaceae. It is endemic to the Sonoran Desert in Southern Arizona, Southern California and Northern Mexico [1]. Cultivation of the jojoba shrub has been established in many desert and semi-desert areas. Its mature seeds are a hard oval, dark brown in color and represent the economic part of the jojoba shrub [2]. Jojoba seeds have a unique storage lipid wax consisting of esters formed from acids and alcohols [3]. Jojoba oil is distinguished by its lack of odor, purity and heat-resistant lubricating properties. Therefore, it is suitable as a basic feedstock in the chemical industry [1] such as pharmaceuticals, lubricants, gear additives, extenders, anti-foaming agents as well as in the wax and polish industries [3,4].
There are a lot of factors that control the growth, flowering and fruiting of jojoba shrubs and their seed yield and consistency, such as plant growth regulators and nutrients [5,6]. Fertilizers are any organic or inorganic material of natural or synthetic origin that is added to soil to supply one or more plant nutrients essential for plant growth. On the other hand, the use of inorganic fertilizers is not only high cost, but is also associated with environmental pollution and soil degradation. In recent decades, the innovative view of agricultural production is attracting the increasing demand for bio-organic fertilizers [7,8].
Thus, agricultural farming practices have been evolving towards environmental friendly systems. It means the reduction in chemical inputs without decreasing the quantity or quality of crop yield.
Mycorrhizal fungi can be integrated with soil management to achieve low-cost sustainable agricultural systems [9]. They are used as biofertilizers which constitute a group of root obligate biotrophs that exchange mutual benefits with about 80% of plants [10][11][12][13]. The host plants perform the photosynthesis process and supply the fungus with soluble carbon. On the other hand, the fungus increases the surface contact of the roots with the soil and promotes plant growth, as it enhances the uptake of immobile nutrients such as phosphorus, nitrogen, copper and zinc as well as increasing water absorption [14,15]. The elements are absorbed through the fungus by a specific absorption system and transferred to the plant via the innate contact appendages and intracellular structures; it is assumed that inducing the transfer of nutrients during the symbiosis process enhances the movement of nutrients to the plant. In addition, mycorrhizal fungi increase the resistance to drought and salinity stresses as well as pests and soil-borne diseases and improve growth and osmotic adjustment through stimulating the production of growth-regulating substances [16]. In this field, many studies such as those by Davies et al. [17] on pepper, Ruiz-Lozano and Azcon [18] on lettuce, Auge et al. [19] on the common bean, Ruiz-Lozano et al. [20] on soybean, Ortas [21] on maize, wheat and cotton, Karandashov and Bucher [22], Roldan et al. [23] on Juniperus, Ortas and Rattan [24] on pepper, wheat and maize, Tobar et al. [25] on lettuce, Igiehon et al. [26] on soybean, Jabborova et al. [27] on spinach, Jabborova et al. [28] on ginger and Zewail et al. [29] on stevia were conducted.
Moringa (Moringa oleifera L.) belongs to the Moringaceae family. Moringa leaves extract is a rich source of macro and micronutrients, amino acids, vitamins, ascorbates, phenolic compounds antioxidants and growth-regulating hormones such as auxins, cytokinins, gibberellins, jasmonic and salicylic acids [30][31][32][33][34]. Thus, it possesses the potential to promote plant growth; hence, it can be used as a natural plant growth promotor. The studies by El-Serafya and El-Sheshtawy [34] on fennel, Prabhu et al. [35] on basil, Ali et al. [36] on geranium and Ahmad et al. [37] on freesia reported that using Moringa oleifera leaves extract as a biostimulant could enhance plant growth, yield and chemical composition of plants.
Using organic agriculture regulations for medicinal and aromatic plant cultivation increases Egyptian trade with European Union [38]. To the best of our knowledge, no reports have been conducted on the combined impact of mycorrhizal fungi and Moringa oleifera leaves extract on jojoba shrubs. Therefore, the aim of this study was to study the influence of mycorrhizal fungi and Moringa oleifera leaves extract as well as their combination treatments on the growth, yield and chemical composition of the jojoba shrub (Strain EAI) under an organic agriculture system.

Materials and Methods
This experiment was conducted to find out the effect of ground inoculation of arbuscular mycorrhizal fungi inoculums of Glomus mosseae and Glomus fasciculatum and foliar application of Moringa oleifera leaves extract and on jojoba shrubs (Simmondsia chinensis), at a private farm in the EL-Behira governorate, Egypt. Arbuscular mycorrhizal fungi were obtained from the Agriculture Research Center in Egypt. A total of 20 g L −1 arbuscular mycorrhizal fungi (approximately 2400 spores) were added as a ground addition. Each shrub was given 3L at the beginning of every season. Jojoba shrubs of similar vigor, age (twelve years old) and size were selected for the application of studied treatments during the two studied seasons (2018/2019 and 2019/2020). The experiment laid out is a factorial arranged in randomized complete block design (RCBD) with three replications. For each replication of treatment, the same shoot concerning height, thickness, vigor and number of fruit and orientation was selected. Distances between rows and within shrubs in rows were 3 and 2.5 m, respectively. A drip irrigation system was applied in the orchard, weed, pest control and fertilization conducted following the standard agro-management practices.
The orchard soil analysis is given in (Table 1), and water irrigation analyses are given in (Table 2) according to the procedures.

Moringa Oleifera Leaves Extract Preparation
For preparing aqueous extract solutions of Moringa oleifera leaves, the plant material was dried in an oven at 70 • C for 48 h. Then, the dried material was ground in a grinder and passed through a 40-mesh screen. To prepare the extracts, 100 g of each ground plant material were macerated in 1000 mL distilled water. Solutions were placed in an orbital shaker at room temperature for 24 h. The extracts were filtered using Whatman filter paper No.1 [39]. The obtained extracts were diluted in order to achieve the concentrations. The analysis of Moringa leaves extract is shown in Table 3. This extract was used to achieve the different analyses and treatments. Extract analyses are given in (Table 3).

Treatments
Arbuscular mycorrhizal fungi at 0 and 20 g L −1 was added to soil in the beginning of the experiment. For fungi inoculate, 3 L containing 20 g L −1 arbuscular mycorrhizal fungi (approximately 2400 spores L −1 ) were added as ground addition for every shrub at the beginning of every season (at the beginning of December).
Treatments of Moringa leaves extract were performed by spraying 4 L per tree every two weeks from the beginning of December. Moringa oleifera leaves treatments were 0, 10, 20, 30 g L −1 leaves extract. The combined treatments are presented in Table 4.

Parameters
For each treatment, nine shrubs (three shrubs for every replicate) were tagged by random selection.

Vegetative and Reproductive Measurements Branch Characteristics
Branch length (cm), length of secondary branches per every branch (cm), number of nodes forming branches per meter, number of secondary branches on the main branch per meter.

Flowering, Fruiting and Seed Yield
Three branches per tree from each treatment were tagged in December 2018 and 2019, and the full bloom date, flowering percentage, final fruit set percentage and seed yield (g tree −1 and kg ha −1 ) were recorded as mentioned by Atteya et al. [6].

Chemical Analyses of Seed
Jojoba oil was extracted from seeds with the Soxhlet extraction method with hexane solvent, and it was calculated as oil percentage (%) and yield per hectare (l ha −1 ). Additionally, crude protein (%), mineral (%) and total carbohydrate (%) were performed following the AOAC [40]. The seeds' fixed oil analyses were performed as mentioned by Atteya et al. [6].

Chlorophyll a and b
They were determined according to Wintermans and Mats [41].

The Macro Elements (N, P and K)
They were determined in leaves according to the method of Chapman and Pratt [42].

Statistical Analysis
Analysis of variance with SAS software [43] was carried out on the test treatment data. Treatments' means were compared using the LSD test at 5% level of probability. Data in Tables are mean value ± SE.

Growth Parameters
Vegetative parameters of jojoba shrubs varied according to ground inoculation of arbuscular mycorrhizal fungi and foliar application of Moringa oleifera leaves extract and their interaction. Tables 5 and 6 indicates that jojoba shrubs treated with arbuscular mycorrhizal fungi recorded an increment in the main branch length (19 and 20%), secondary branch length (40.6 and 40.3%), number of branched nodes (27.42 and 27.69%) and number of secondary branches (35.62 and 35.48%) compared with corresponding untreated control shrubs in both seasons. With comparing the spraying levels of Moringa oleifera leaves extract, the application of 30 g L −1 gave the maximum significant mean values of shrub growth parameters with increment percent of 35 and 35% for the main branch length, 64 and 64% for secondary branch length, 42 and 41% for the number of branched nodes and 53 and 53% for the number of secondary branches compared with control shrubs. The interaction was significant between Moringa oleifera leaves extract and ground inoculation of arbuscular mycorrhizal fungi treatments. Moreover, the maximum significant mean values of jojoba shrub growth were found with the combination treatment of arbuscular mycorrhizal fungi (20 g L −1 ) plus 30 g L −1 Moringa oleifera leaves extract in both seasons, as this treatment recorded an increment percentage (57 and 58%), (110 and 111%), (73 and 72%), (92 and 93%) for main branch length, secondary branch length, number of branched nodes and number of secondary branches, respectively, compared with control treatment in both studied seasons.
Data are mean value ± SE. Means in columns followed by the same letter are not statistically different at the 0.05 significance level.

Flowering and Fruits Set Parameters
Data shown in Tables 6 and 7 are clear that foliar application of ground inoculation of arbuscular mycorrhizal fungi and Moringa oleifera leaves extract, as well as their combined treatments, significantly promoted all flowering and fruits set parameters compared with untreated control treatment. When using mycorrhizal fungi, the reduction in full bloom date was 13% while the increment in flowering and final fruit set percent was 15 and 4%, respectively, compared with untreated shrubs. Flowering and fruits set parameters were significantly improved using 30 g L −1 Moringa oleifera leaves extract compared with the other extract concentrations and control. The reduction in full bloom date was 20% and the increment in flowering and final fruit set percent was 25 and 7%, respectively, compared with control shrubs. Regarding the combined treatments, the most significant increases in flowering and fruits set parameters were obtained at the combination treatment of arbuscular mycorrhizal fungi (20 g L −1 ) plus 30 g L −1 Moringa oleifera leaves extract in both seasons. Moreover, it achieved a reduction percent in full bloom date with 20%, while it achieved an increment in flowering and final fruit set percent with 25 and 11%, respectively. Table 7. The mean values of the final fruit set (%), seed yield (g plant −1 ) and seed yield (Kg ha −1 ) of jojoba shrubs as affected with ground inoculation of arbuscular mycorrhizal fungi and foliar application of Moringa oleifera leaves extract, as well as their combination treatments in both seasons of the study.

Seeds Yield
Seeds yield per tree and per hectare of jojoba shrubs have been significantly affected with the application of both ground inoculation of arbuscular mycorrhizal fungi and Moringa oleifera leaves extract (Table 7). Therefore, using arbuscular mycorrhizal fungi had a 13% increment in seed yield per tree and per hectare of jojoba shrubs, respectively, in both seasons compared with the control. Additionally, foliar application of 30 g L −1 gave a maximum increment in seed yield per tree (21%) and per hectare (21%) compared with control. Likewise for the combined effect, the treatment of arbuscular mycorrhizal fungi (20 g L −1 ) plus 30 g L −1 Moringa oleifera leaves extract recorded the maximum significant seed yield per tree and per hectare in both seasons. In addition, it recorded the maximum increment percentage compared with the control treatment for the yield of seeds per tree (35%) as well as per hectare (35%) in both seasons, respectively. The most pronounced effect was recorded with the application of ground inoculation of arbuscular mycorrhizal fungi and 30 g L −1 Moringa oleifera leaves extract for combination treatment. Tables 8 and 9 show the effect of arbuscular mycorrhizal fungi and Moringa oleifera leaves extract rates on chemical seed compounds of jojoba shrubs. Arbuscular mycorrhizal fungi application caused significant maximum increases in minerals, proteins and fixed oil percent with a significant decrease in carbohydrate percentage. Amendment of soil with arbuscular mycorrhizal fungi increased the percent of minerals, proteins and fixed oil percent by 17%, 9% and 2% compared with the inoculated plants. The maximum carbohydrate percentage was 27.28% in the first season in the control and 25.16% in the second season. For the application of Moringa oleifera leaves extract, foliar application of 30 g L −1 had significant maximum increases in minerals, proteins and fixed oil percentage by 25%, 14% and 8%, while the control shrubs recorded the maximum carbohydrate percentage in the jojoba seeds (25.61 and 23.45%) in the first and second seasons, respectively. Regarding the combination treatments, all treatments improved minerals, proteins and fixed oil percent in jojoba seeds in both seasons compared with the control. In addition, the treatment of arbuscular mycorrhizal fungi (20 g L −1 ) plus 30 g L −1 Moringa oleifera leaves extract gave the significant maximum increases in mineral and protein percentages, as it increased the percent of minerals and proteins percentage by 42% and 23%, respectively, above the control in both seasons. For the fixed oil percentage, the treatment of uninoculated soil plus 30 g L −1 Moringa oleifera leaves extract had the maximum percent which was above the control treatment with 14% in both seasons. On the contrary, the maximum carbohydrate percentage (27.28 and 25.16%) in both seasons, respectively, was found with the shrubs of the control treatment.    Table 9 shows the effect of arbuscular mycorrhizal fungi and Moringa oleifera leaves extract on jojoba seeds oil content per shrub and yield per hectare. Amendment of soil with arbuscular mycorrhizal fungi caused significant increases in jojoba seed oil content per shrub and yield per hectare. The yield of jojoba seeds oil content per shrub and yield per hectare ranged between 1158 and 1322 mL shrub −1 and 1763 and 1544 L ha −1 in the first season and between 1226 to 1400 mL shrub −1 and 1635 to 1867 L ha −1 in the second season. The highest significant values of jojoba seed oil content per shrub and yield per hectare were noticed with the soil amended with arbuscular mycorrhizal fungi, while the lowest values were found with the control soil. Significantly, the application of Moringa oleifera leaves extract with 30 g L −1 had the maximum jojoba seed oil content per shrub and yield per hectare, as it increased the seeds' oil content per shrub and yield per hectare by 30% and 30%, respectively, compared with non-foliar shrubs. For the combination treatments, arbuscular mycorrhizal fungi (20 g L −1 ) plus 30 g L −1 Moringa oleifera leaves extract gave the maximum significant jojoba seed oil content per shrub and yield per hectare, as it increased seed oil content per shrub and yield per hectare by 42% and 42%, respectively, above untreated shrubs.

Seeds Fixed Oil Analysis
It was noticed from Table 10 that the fixed oil of jojoba seeds is rich with unsaturated fatty acids, which ranged between 87.99 and 95.88% for T7 and control treatment. The total known components ranged between 91.82 to 97.94% for T7 and control, respectively. The major fatty acids in jojoba oil are Gadoleic acid (48.21-52.29% for T3 and T1), Oleic acid (12.03-14.58% for T6 and control), Erucic acid (12.68-15.28% for T7 and T4) and Nervonic acid (11.7-12.78% for T2 and T1).

Chlorophyll a and b
All treatments of arbuscular mycorrhizal fungi and Moringa oleifera leaves extract and their interaction recorded significant increases on chlorophyll a and b of jojoba leaves in both seasons (Table 11). Amendment of soil with arbuscular mycorrhizal fungi caused remarkable increases in chlorophyll a and b (Table 11). Chlorophyll a and b increased by 7% and 6% in shrubs of treated soil compared with shrubs of untreated soil in both seasons. For the effect of Moringa oleifera leaves extract, the maximum significant values of chlorophyll a and b in the leaves of jojoba shrubs were obtained from 30 g L −1 Moringa oleifera leaves extract while the minimum ones were obtained from the control treatment. Chlorophyll a and b were higher by 9% and 9% compared with that sprayed with distilled water. Regarding the combination treatments, the inoculated soil plus using the highest rate of Moringa oleifera leaves extract (30 g L −1 ) gave the maximum mean values of chlorophyll a and b. These values increased by 16% and 17% for chlorophyll a and b, respectively, compared with the control treatment in the two seasons. Table 11. The mean values of chlorophyll a and b (mg g −1 ) of jojoba shrub as affected with ground inoculation of arbuscular mycorrhizal fungi and foliar application of Moringa oleifera leaves extract as well as their combination treatments in both seasons of the study.

Macro Elements (N, P and K)
Nitrogen, phosphorus and potassium in jojoba leaves were measured and Table 12 show these results. The application of arbuscular mycorrhizal fungi affected significantly nitrogen, phosphorus and potassium content in the leaves of jojoba shrubs. Nitrogen, phosphorus and potassium percentage in the inoculated shrub was higher than that of uninoculated shrubs by 6%, 12% and 3% in both seasons. Moringa oleifera leaves extracts significantly increased N, P, K percentage in jojoba leaves. The application of 30 g L −1 Moringa oleifera leaves extract recorded the maximum mean percentage of nitrogen (9%), phosphorus (18%) and potassium (6%) in jojoba leaves in both seasons. Likewise for the combined effect, the treatment of arbuscular mycorrhizal fungi (20 g L −1 ) plus 30 g L −1 Moringa oleifera leaves extract recorded the maximum significant nitrogen, phosphorus and potassium in jojoba leaves in both seasons. In addition, it recorded the maximum increment percentage compared with the control treatment for nitrogen (16%), phosphorus (33%) and potassium (9%) in both seasons.

Effect of Arbuscular Mycorrhizal Fungi
By comparing between inoculated and uninoculated jojoba shrubs with arbuscular mycorrhizal fungi, it was noticed that inoculated shrubs were outperformed in all studied parameters of growth, flowering, fruits set, chlorophyll a and b content and N, P, K percentages. Arbuscular mycorrhizal fungus application increases photosynthesis efficiency and chlorophyll pigment content to increase biomass production [44,45]. Meanwhile, it improves flowering and fruits set. This improvement is due to the role of arbuscular mycorrhizal fungi in increasing absorption of water and nutrients, and enhances transport of immobile mineral elements through exploring larger volumes of the soil by mycorrhizal hyphae to stimulate the growth of plants [46]. These results are in harmony with studies by Davies et al. [17] on pepper, Ruiz-Lozano and Azcon [18] on lettuce, Auge et al. [19] on the common bean, Ruiz-Lozano et al. [20] on soybean, Ortas [21]  Despite the decreasing carbohydrate percentage in seeds, all the minerals, proteins and fixed oil percentages were significantly increased in treated shrubs compared with the control (uninoculated shrubs). This may be due to the role of arbuscular mycorrhizal fungus as a biostimulant in increasing the levels of macronutrients in the plant. On the other hand, the mycorrhizal symbiosis reduced the carbohydrate content of the plants compared with the control plants as it receives between 4 and 20% of photosynthetically fixed carbon from the host plant [48]. Moreover, the fungus provides the host plant with nutrients and stimulates the plant metabolism to build a high level of protein content, which may cover the plant requirements and boost the immune system, thus increasing the abiotic and biotic stress resistance of the host plant [49][50][51]. These results are supported by Plenchette et al. [52], who reported that the benefits of arbuscular mycorrhizal fungus are mainly attributed to improving phosphorous nutrition. Both Van der Heijden et al. [53] and Allen [54] found that arbuscular mycorrhizal fungi improve plant productivity, plant nutrition, soil structure and water uptake under semi-arid conditions. Auge [55], Al-Karaki [16], Javaid [10], Khaosaad et al. [56] and Azcón et al. [57] found that arbuscular mycorrhizal fungi induced plant hormone production to increase plant photosynthesis, improve osmotic adjustment under normal, drought and salinity stresses and increase resistance to pests and soil-borne diseases against biotic and abiotic stresses.
Seed yield and oil yield of jojoba shrubs attributed to arbuscular mycorrhizal fungus in this study may be related to the role of the fungus in biostimulantion of increasing levels of macronutrients in the plant and phosphorous nutrition [52]. The increase in yield due to phosphorous nutrition may be attributed to the activation of metabolic processes, where its role in building phospholipids and nucleic acid is known [58,59]. Phosphorous is a key constituent of ATP and plays a significant role in energy transformation in plant and also plays a role in seed formation. Application of P, Ca and B fertilizers increased nutrient availability to the crops during the growing season, which leads to greater utilization of assimilates into the fruits and ultimately increased the number of filled fruits and set percentage [60]. Therefore, oil content in seeds increased gradually with increasing levels of phosphorus [59,61,62]. Priya et al. [63] and Atteya et al. [64] reported that the increase in oil content and oil yield is attributed to the application of a higher level of N, P and K to the crop. Giri et al. [65] noticed that using arbuscular mycorrhizal fungi increased the oil content of Dutch fennel plants.

Effect of Moringa Oleifera Extract
The increase in improvement of growth, flowering, fruits set, chlorophyll a and b content and N, P, K percentages was in parallel with increasing the concentration of Moringa oleifera leaves extract concentration. This may be due to the collective performance of macro and micronutrients, amino acids, vitamins, ascorbates, phenolic compounds antioxidants and growth-regulating hormones such as auxins, cytokinins, gibberellins, jasmonic and salicylic acids present in Moringa leaves extract [30][31][32][33][34]. This improvement in vegetative growth and flowering was reflected in the seed yield and oil yield of jojoba shrubs in this study. Fruits are the main sink of plant production. Gibberellins increase sink demand by increasing fruit cell elongation and enhancement of phloem unloading or/and metabolism of carbon assimilates in fruit, as gibberellins induce activities of sugar metabolizing enzymes. Therefore, it finally increases seed and oil yields [6,66,67]. Our results are in agreement with many researchers, as Moringa leaves extract has been reported to increase the growth, yield and chemical composition of many crops such as maize, rice, sorghum, wheat [7,68], tomato [69], basil [35], common bean [33], roselle [70], geranium [36], freesia [37] and fennel [34].

Effect of Combination Treatments
The increase in herb content of nitrogen, phosphorus and potassium may be due to Moringa leaves extract, which is a rich source of amino acids, potassium, calcium, iron, vitamin E, ascorbates, phenolic compounds and growth-regulating hormones such as zeatin [30,32]. Additionally, arbuscular mycorrhizal fungi improved the productivity of plants, which was attributed to enhanced uptake of immobile nutrients such as phosphorus, zinc, nitrogen and copper. This increase in the presence of zeatin of Moringa leaves extract increased chlorophyll a and b content and the photosynthesis process. Therefore, in this study, vegetation and flowering as well as fruits set were improved, and then the yield of seeds and oil was improved too as a result of combination treatments of biological and stimulating fertilizers. These results agree with El-Serafya and El-Sheshtawy [34] on fennel, Mervat et al. [71] on grapevines, Djouhou et al. [46] on cowpea and sorghum and Djouhou et al. [72] on Moringa. Atteya et al. [64] reported that fertilization treatments enhanced seed and oil yields of the Moringa oleifera tree and affected fatty acid percentages. Atteya et al. [6] reported that jojoba seeds contain approximately 53.68% fixed oil percentage, and the main fatty acid is Gadoleic acid (C20:1), which represents 53.54%; the second, Oleic acid (C18:1), represents 15.59%. Genaidy et al. [73] reported that the Strain EAI of jojoba shrubs is distinguished by a high yield of seeds and oil.

Conclusions
We can conclude from this study that the application of both mycorrhizal fungi and Moringa oleifera leaves extract is recommended to increase seed and oil yields of the jojoba shrub. Moreover, the application of combination treatment of 20 g L −1 mycorrhizal fungi plus 30 g L −1 Moringa oleifera leaves extract recorded the maximum mean values of main branch length, length of secondary branches, number of branched nodes, number of secondary branches, flowering percentage, final fruit set percentage, seed yield per shrub and per hectare, percentage of minerals, proteins as well as oil yield per shrub and per hectare, chlorophyll a and b, N, P, K percentage with a minimum mean value of a number of days until full bloom in both seasons. The maximum percentage of gadoleic fatty acid was found with the combination treatment of 0 g L −1 mycorrhizal fungi plus 10 g L −1 Moringa oleifera leaves extract.

Funding:
The study did not receive any external financing.

Institutional Review Board Statement: Not applicable.
Informed Consent Statement: Not applicable.

Data Availability Statement:
The data presented in this study are available within the article.