fermentation

: Cell inﬂammation disrupts intestinal barrier functions and may cause disorders related to a leaky gut, possibly leading to diabetes. The objective of this study was to determine if carao ( Cassia grandis ) incorporated into yogurt enhances in vitro intestinal barrier function. To achieve this goal, Caco-2 cells were used as a model of intestinal barrier permeability. Caco-2 cells were treated with cytokines (interleukin-1 β , tumor necrosis factor-α , interferon-γ , and lipopolysaccharide (LPS)) and yogurt with carao yogurt (CY) at different doses (1.3 g/L, 2.65 g/L, and 5.3 g/L). Real-time quantitative polymerase chain and immunoﬂuorescence microscopy were applied to evaluate the expression and localization of tight junction proteins. Functional effects of the formulation of yogurt supplemented with carao were also evaluated in terms of the antioxidant activity, the α -glycosidase activity, and lipase inhibitory properties. In addition, the hypoglycemic potential was validated in vivo in a rat model. Compared to the control yogurt, Caco-2 TEER (transepithelial electrical resistance evaluation) by yogurt with 5.3 g/L of carao was signiﬁcantly lower ( p < 0.05) after 48 h. Yogurt with 5.3 g/L of carao had a considerably lower permeability ( p < 0.05) than control yogurt in FD and LY ﬂux. Yogurt with 5.3 g/L of carao enhanced the localization of ZO-1. Carao addition into yogurt increased the ﬂavonoid content, apparent viscosity, lipase inhibition activity, and α - glycosidase activity. The rats fed with the yogurt with 5.3 g/L of carao demonstrated a higher blood glucose modulation.


Introduction
The cause of leaky gut syndrome is not clear. The digestive system is susceptible to stimuli in many people with this disorder. Affected people feel discomfort caused by intestinal gas or contractions that others would not find problematic. For some people, high-calorie foods or high-fat diets may be the trigger. Since many food products contain multiple ingredients, it is difficult to identify the specific precipitating factor.
How the precipitating factors cause the syndrome is not evident. Emotional factors (stress, anxiety, depression, and fear), drugs (including laxatives), or hormones can trigger or worsen the leaky gut syndrome. Affected people do not always have symptoms after a common trigger, and symptoms often appear without an obvious trigger [1]. On the other

Yogurt Preparation
Yogurt was produced as that of Aleman et al.'s work (2023) [25]. A total of 2% fat bovine milk obtained from a local supermarket was mixed with carao powder at different concentrations (0 g/L (control), 1.3 g/L, 2.65 g/L, and 5.3 g/L) and kept under constant stirring for 1 h at 10-15 • C. Subsequently, the milk was pasteurized at 85 • C for 30 min and, carao was then mixed vigorously with a commercial immersion blender (Waring Commercial, McConnellsburg, PA, USA) for 3 to 5 min while the temperature was held at 80 • C. Later, 3 mL of each culture of S. thermophilus ST-M5 and L. bulgaricus LB-12 (Chr. Hansen, Milwaukee, WI, USA) were incorporated into the milk (3 gallons) at 41 • C, and the milk mixture was then incubated at 40 ± 1 • C and kept until the fermentation pH reached 4.6. The mixture was poured into labeled 355 mL Reynolds RDC212-Del-Pak Combo-Pak containers (Alcoa, Inc., Pittsburgh, PA, USA) and stored at 4 • C.

Technological and Microbial Analysis
The viscosity, titratable acidity (TA), pH, and microbial counts were determined at 0, 2.5, 5, and 7 h during incubation, respectively. The apparent viscosity of yogurt was evaluated by using Brookfield rotational DV-II viscometer (Brookfield Engineering Lab Inc., Stoughton, MA, USA) on a helipad stand and using a T-C spindle rotated at 20 rpm. The pH was measured by using a pH meter (Thermo Orion 3-Star Benchtop pH Meter, Fisher Scientific, Pittsburgh, PA, USA). TA was achieved by using 9 g of yogurt with 9 mL of distilled water and 0.5 mL of phenolphthalein, and samples were titrated with 0.1 N NaOH [26]. Yogurts were serially diluted in sterilized peptone (0.1% wt/v) from 10 −1 to 10 −6 and pour plates in duplicate recording were incubated [27] S. thermophilus was enumerated by using S. thermophilus (ST) agar (formulated with sucrose, K2HPO4, Bacto yeast extract, Bacto tryptone and agar) adjusted to pH 6.8 and incubated at 37 • C for 48 h aerobically, and L. bulgaricus (LB) was enumerated by using Lactobacillus MRS agar (formulated with MRS broth powder and agar), adjusted to pH 5.2 and incubated at 43 • C for 72 h anaerobically. Colonies were counted by using a Quebec Darkfield colony counter (Leica Inc., Buffalo, NY, USA). The counts were transformed as logarithms of the number of colony-forming units per mL Log (CFU/mL). All experiments were conducted in triplicates.

Antioxidant Determination, Total Phenolics, and Flavonoid Content
The yogurt was first frozen to −20 • C, and then placed in a FreeZone (Labconco) freeze dryer unit for~72 h. The freeze-dried yogurt was then pulverized using a knife mill Retsch SM 100 (Retsch GmbH, Haan, Germany) (501-700 mm). Later, 200 mg of ground lyophilized yogurt was placed in a 30 mL centrifuge falcon tube with 20 mL of 80% methanol solution. The obtained mixture was mixed and sonicated for 20 min. The sonicated solution was then centrifuged for 7 min at 1500× g force. The supernatant was filtered for antioxidant determination, total phenolics, and flavonoid content.

Enzymatic Inhibition Assays
The α-glucosidase inhibitory activity was applied according to the reference method with slight modification [30]. Yogurt with carao at different concentrations (0 g/L (control), 1.3 g/L, 2.65 g/L, and 5.3 g/L) were tested. In 96-microwell plates, 50 µL of yogurt samples (YS) contained 0.5 mg/mL for YS solution and α-glucosidase from Saccharomyces cerevisiae (100 µL) (0.6 U/mL) in 0.1 M phosphate buffer pH 6.9. The obtained solution was incubated for 10 min at 37 • C. An amount of 50 µL of 3 mM p-nitrophenyl-α-glucopyranoside (NPG) in phosphate buffer pH 6.9 (NPG) was then mixed and incubated again for 13 min at 37 • C. The α-glucosidase inhibitory activity was evaluated using a spectrophotometer (Synergy HT, Bio-Tek Instruments, Inc., Winooski, VT, USA) at a wavelength of 405 nm. An amount of 1 mM acarbose (Sigma-Aldrich, Taufkirchen, Germany) was used as a control. The α-glucosidase activity (I) was calculated using Equation (2): where AS is the difference between the absorbance of the sample and the absorbance of the blank and AC is the difference between the absorbance of the control and the absorbance of the blank. The lipase inhibitory activity was observed by measuring the production of pnitrophenol (pNP), resulting from the hydrolyzation of p-nitrophenol palmitate (pNPP) [30]. In 96-microwell plates, 20 µL YS comprising 0.5 mg/mL yogurt solution and 20 µL lipase (from the porcine pancreas) solution (1 mg/mL) in 0.1 M sodium phosphate buffer adjusted to pH 6.9 was incubated for 11 min at 37 • C. Then, 1800 µL of sodium phosphate buffer 0.1 M comprising 20 µL of p-nitrophenyl palmitate in isopropanol (0.01 M), Arabic gum (0.55 mg/mL), and sodium cholate (1.15 mg/mL) were combined and incubated at 37 • C for 11 min. The content of p-nitrophenol was measured by using a spectrophotometer (Synergy HT, Bio-Tek Instruments, Inc., Winooski, VT, USA). The lipase inhibitory activity was calculated by Equation (2). All experiments were performed in triplicates.

Transepithelial Electrical Resistance
Cell monolayer integrity influenced by the yogurt samples with and without carao (0 g/L (control), 1.3 g/L, 2.65 g/L, and 5.3 g/L) were evaluated by using transepithelial electrical resistance evaluation (TEER) [34]. The inflammatory stimulus (I) containing interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interferon-gamma (IFN-γ), and lipopolysaccharide (LPS) was applied with yogurt samples, and I with 100 µM genistein media (positive control) was also evaluated. Growth media were transferred from culture plates and inserts, and cells were washed with Hank's balanced salt solution (HBSS) containing 5 mM HEPES combined with the basolateral and apical compartments in different volumes (1 and 0.2 mL, respectively). Later, the plates were kept in 5% CO 2 , at 37 • C, and for 25-30 min. Plates were then transferred to a hot plate at 39 • C and TEER was estimated by using an Epithelial Volt/Ohm (TEER) Meter 3 with an EVOM3 chopstick electrode (World Precision Instruments, Sarasota, FL, USA). An electrode was kept under the standard condition of not touching the monolayer. Samples without cells were used as blank and differentiated monolayers. TEER (Ω·cm 2 ) values were from 550-700 ohm cm 2 (n = 25). Triplicated measurements were taken. All experiments were performed in triplicates.

Paracellular Permeability
Paracellular permeability as influenced by the yogurt samples with and without carao (0 g/L (control), 1.3 g/L, 2.65 g/L, and 5.3 g/L) were evaluated by applying the flux of fluorescein isothiocyanate (FITC)-dextran 4000 (FD) and Lucifer yellow (LY) through the cell monolayers based on a literature review [35,36]. The inflammatory stimulus (I) containing interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interferon-gamma (IFN-γ), and lipopolysaccharide (LPS) was applied with yogurt samples, and I with 100 µM genistein media (positive control) was also evaluated. FD (1 mg mL −1 ) and LY (0.5 mg mL −1 ) were mixed with HBSS/HEPES at 37 • C and 0.2 mL of it was added into the apical compartment, whereas 1.0 mL of HBSS was added to the basolateral well. The covered plates were incubated at 37 • C and 150 rpm. An amount of 300 µL of the basolateral chamber was transferred into a black, clear-bottom 96-well plate (Corning Costar, New York, NY, USA) every 4 h for 16 h. Later, the basolateral compartment was rinsed and a new 1 mL of HBSS was added at 37 • C. The fluorescence strength was measured by using a fluorescence spectrophotometer PLX800 (BioTek, Winooski, VT, USA) under excitation/emission wavelengths of 485/530 nm (FD) and 428/540 nm (LY). The apparent permeability coefficient, P app (cm/s), was measured according to Equation (3): where C is the initial concentration on the apical side (mol/mL), dQ is the portion of the fluorescent marker on the basolateral side (mol/mL), dt is the flux per second (1/s), and A is the membrane surface area (cm 2 ). All experiments were performed in triplicates.

Immunofluorescence Microscopy
The immunofluorescence microscopy as influenced by the yogurt samples with and without carao (0 g/L (control), 1.3 g/L, 2.65 g/L, and 5.3 g/L) were evaluated [34]. The inflammatory stimulus (I) containing interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interferon-gamma (IFN-γ), and lipopolysaccharide (LPS) was applied with yogurt samples and cells were also treated with only the growth media (healthy cells). The cells were grown in the Lab-Tek II chamber slide system (Nalge Nunc International, Rochester, NY, USA) and with PBS (without Ca and Mg) and fixed with 3% paraformaldehyde. Cells were overlaid with primary antibody claudin-1, occluding, and Zo-1 (Zymed Laboratories, San Francisco, CA, USA), which were diluted 1:50 and incubated for 1 h. Following incubation, secondary antibodies 1:100 (goat anti-rabbit IgG antibody, (H + L) FTIC conjugate; Sigma) were added to fixed cells and incubated for 30 min. Next, tight junction protein expression was visualized using a fluorescent light microscope with FITC-compatible media (Compound Microscope Leitz Optilux, Stuttgart, Germany). Images were recorded and examined using ZEN 2010 software (Carl Zeiss AG, Oberkochen, Germany). At least ten fields were viewed and selected based on balanced staining, and the square cutout borders were withdrawn.

Transmission Electron Microscopy
The transmission electron microscopy (TEM) influenced by the yogurt samples with and without carao (0 g/L (control), 1.3 g/L, 2.65 g/L, and 5.3 g/L) were evaluated [37]. The inflammatory stimulus (I) containing interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interferon-gamma (IFN-γ), and lipopolysaccharide (LPS) was applied with yogurt samples and cells were also treated with only the growth media (healthy cells). An amount of 1 µm of cells in different treatments were collected by applying glass knives on an ultramicrotome (Reichert Ultracut S, Minnesota, MN, USA) after dehydration and inserting in Araldite epoxy resin and colored with 1% toluidine blue in 1% sodium borate (Sigma-Aldrich, Taufkirchen, Germany). The areas which were selected (∼90 nm in thickness) for examination were cut by applying a diamond knife and colored with saturated uranyl acetate and Reynold's lead citrate (Sigma-Aldrich, Taufkirchen, Germany). Digital images (from 10 different clear spots) were collected from Samples that were observed by using TEM (Hitachi H7000, 100 kV, Yokohama, Japan). At least three images from each treatment group were analyzed. All experiments were conducted in triplicates.

Gene Expression Analysis of Tight Junction Proteins
The gene expression analysis of tight junction proteins influenced by the yogurt samples with and without carao (0 g/L (control), 1.3 g/L, 2.65 g/L, and 5.3 g/L) were evaluated [38]. The inflammatory stimulus (I) containing interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interferon-gamma (IFN-γ), and lipopolysaccharide (LPS) was applied with yogurt samples and cells were also treated with only the growth media (healthy cells). RNA extraction from the Caco-2 cells method was applied by using a slight modification [34]. According to the methodology, 5 × 10 6 cells were collected using a centrifuge, and DNaseI from Ambion DNA-free TM Kit (Thermos Fisher Scientific, Waltham, MA, USA) was added to them for cleaning DNA residuals. RevertAid RT kits were applied to observe reversed transcription according to protocol. Qubit (Thermo Fisher Scientific, Waltham, MA, USA) was applied to define RNA concentration by using fluorometric quantitation. A total of 1 µg of isolated RNA as control was selected, and synthesized cDNA was amplified in 7500 real-time PCR using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific, Waltham, MA, USA).

Animal Models and Glucose Tolerance Evaluation
The established animal experiment guidelines by the Declaration of Helsinki were used as a reference for the animal models, which is approved by the Ethics Committee in Food Research at the Honduran Association of Medicine and Nutrition (ASOHMENU) with form number AS-ASHOMENU-0013-2022.
Forty-nine healthy five-week-old male Wistar rats were purchased from Universidad Nacional Autonoma de Honduras, Tegucigalpa (Honduras). Rats were divided into Fermentation 2023, 9, 566 7 of 25 7 groups, fed with YS (I-IV) (carao with 0 g/L (I), 1.3 g/L (II), 2.65 g/L (III), 5.3 g/L (IV)), regular diet only (healthy or untreated rats) (V), sucrose solution (diabetic induced) (VI), and metformin (VII). First, the rats were subjected to a diabetes induction by intraperitoneal injection with streptozotocin after one week of adoption at 22 • C, 55% RH, and a regular diet (Ban et al., 2020) [42]. Injection of streptozotocin (streptozotocin freshly dissolved in 50 mmol/L citrate buffer (pH 4.5)) was administered until blood sugar levels were greater than 10.0 mmol/L, which is categorized as diabetes. After induction of diabetes, the rats were fed with highly contained sugar food (30 mg/mL sucrose at the dose of 0.1 g per 100 g body weight given). Diabetic-induced rats received a high-fat diet. Glucose levels were recorded weekly for 6 weeks after feeding. Fasting blood glucose was observed by using a OneTouch Ultra blood glucose meter (Accu-Chek Roche, Mannheim, Germany) after 12 h of fasting every two weeks, using blood collected from the tail vein. The glucose tolerance was observed by an oral glucose tolerance test (Chen et al., 2016) [43]. After 12 h of fasting, rats were injected with 2.5 g/kg glucose. Then, blood sugar samples were collected from the tail vein at 0-, 30-, 60-, and 120-min. The area under the curve (AUC) was calculated using the following Equation (4): where A is the blood sugar.

Statistical Analysis
The statistical analysis was performed as in Aleman et al.'s work (2023) [25]. Proc mixed was used to determine the statistical significance of the carao concentration effect, hour effect, and interaction effect of carao concentration x hour for the technological measurements, microbial evaluations, transepithelial electrical resistance determinations, paracellular permeability observations, and blood glucose levels. In our case study, we used mixed proc model random (replications in yogurt batches) and mixed effect (treatments; carao concentrations), and repeated measures (time). The differences of least square means were used to determine significant differences at p < 0.05. In addition, one-way ANOVA was applied for total phenolics, total flavonoids, antioxidant capacity measurements, gene expression analysis, AUC measurements, fasting blood glucose levels, TEER values, enzymatic activity, and viscosity observations. Significant differences were determined at α = 0. 05. Post hoc tests were conducted with Tukey's HSD. Data were processed using Statistical Analysis Systems SAS (SAS Institute Inc., Cary, NC, USA). Each experiment was carried out in triplicate or otherwise defined.

Technological and Microbial Evaluation
TA values of the yogurt are illustrated in Figure 1a. The main effects of the concentration and hour were significant (p < 0.05), whereas the interaction effect concentration * hour was not significant (p > 0.05). Yogurts with 5.3 g/L of carao had significantly (p < 0.05) higher TA values than control samples. TA remained stable until 2.5 h and significantly (p < 0.05) increased after 5 h during the fermentation.
The pH values of YS during the fermentation process is illustrated in Figure 1b. The time effect was significant (p < 0.05), whereas the concentration effect and the concentration * time interaction effect were not significant (p > 0.05). pH values decreased significantly (p < 0.05) during 0, 2.5, 5, and 7 h of fermentation. The pH values of YS during the fermentation process is illustrated in Figure 1b. The time effect was significant (p < 0.05), whereas the concentration effect and the concentration * time interaction effect were not significant (p > 0.05). pH values decreased significantly (p < 0.05) during 0, 2.5, 5, and 7 h of fermentation. Acidity and pH are the two most important parameters of fermented products, leading to estimated shelf-life. The bacterial starters decompose the lactose into lactic acid, resulting in acidity. Additionally, coagulation in the fermentation process of the yogurt is related to the decrease in pH and rise in total acidity. Similar results were observed in previous studies [44,45]. Although there is no significant difference (p > 0.05) between samples and control at 0 and 2.5 h for TA values, control and treated samples with carao powder demonstrated a significant difference in 7 h of incubation. A significant (p < 0.05) decrease in pH and an increase in acidity were shown over time. The pH was not significantly affected by adding the carao powder, but the total acidity of 5.3 g/L CY was higher than other samples. Acidity and pH are the two most important parameters of fermented products, leading to estimated shelf-life. The bacterial starters decompose the lactose into lactic acid, resulting in acidity. Additionally, coagulation in the fermentation process of the yogurt is related to the decrease in pH and rise in total acidity. Similar results were observed in previous studies [44,45]. Although there is no significant difference (p > 0.05) between samples and control at 0 and 2.5 h for TA values, control and treated samples with carao powder demonstrated a significant difference in 7 h of incubation. A significant (p < 0.05) decrease in pH and an increase in acidity were shown over time. The pH was not significantly affected by adding the carao powder, but the total acidity of 5.3 g/L CY was higher than other samples. Figure 1c,d illustrate S. thermophilus (ST) and L. bulgaricus (LB) counts of YS during 7 h of fermentation. For both bacteria, the time effect was significant (p < 0.05), whereas the concentration effect and the concentration * time interaction effect were not significant (p > 0.05). The ST and LB counts increased significantly (p < 0.05) during 0, 2.5, 5, and Fermentation 2023, 9, 566 9 of 25 7 h of fermentation. Carao powder did not significantly (p > 0.05) affected the ST and LB counts for 7 h. Although 5.3 g/L yogurt showed a slightly higher value (8.5 and 9 Log CFU/mL), there were no significant (p > 0.05) differences among the samples. The ST and LB counts increased gradually, indicating progressive fermentation. It is reported that using bioactive compounds and tropical fruits' pulps such as guava or soursop can maintain the bacterial viability of Lactobacillus rhamnosus and Bifidobacterium animalis after the first day of fermentation, which is due to the bioactive and bactericidal compounds [46]. During the fermentation, the viable counts of ST and LB in CY samples were above the 6.0 Log CFU/mL, indicating that carao does not harm the growth of yogurt starter culture bacteria. It is important that functional ingredients do not interfere with microbial growth in dairy fermented products.
The apparent viscosity of yogurt samples (1.3, 2.65, and 5.3 g/L) are shown in Figure 1e. Apparent viscosity significantly (p < 0.05) increased by adding carao powder at 5.3 g/L when compared to control yogurt. Galactomannan has been found in carao samples [20], and it has been shown to have a great ability to bind water [47]. It is possible that this polysaccharide could increase viscosity in the CY samples. Adding bioactive molecules to yogurt could also play a decisive role in the texture of fermented milk [48]. Table 1 shows the total polyphenols (TPC), total flavonoid content (TFC), and antioxidant activity (AA) values of the yogurt samples. There were no significant difference (p < 0.05) between different CY (1.3, 2.65, and 5.3 g/L) and control samples in AA. The fact that there was no significant antioxidant activity is notice. The content of total polyphenols and flavonoids in 5.3 g/L CY was significantly higher than in control and 1.3 g/L yogurt samples. Carao has a high flavonoid content [20,22]. One primary purpose of plant-based fortification into dairy cultured products is to increased antioxidant activity. Plant-based ingredients are a good source of polyphenols and have a higher phenolic content when compared to animal-based components.

Alpha-Glucosidase and Lipase Inhibition Evaluation
α-Glucosidase and pancreatic lipase digest carbohydrates and lipids, respectively [49]. The activity of enzymatic inhibition was observed in how carao powder regulates alphaglucosidase and lipase activity. Inhibition of α-glucosidase and lipase activity is shown in Figure 2. The most effective CY for lipase inhibition activity (52.11%) was 2.65 g/L CY, which was 6.62% higher than control yogurt, and α-glucosidase inhibition was more effective in 5.3 g/L CY (36.11), which was 10.53% higher than the control. Plants can inhibit α-glucosidase and lipase activity to prevent diabetic complications such as hyperglycemia (diabetes) and hyperlipidemia (metabolic syndrome and heart disease) due to their antioxidant contents and bioactive compounds such as flavonoids [50,51]. Free phenolics of a peanut meal have been shown to inhibit α-glucosidase and pancreatic lipase [48]. Other plants such as Dendrobium, genus of mostly epiphytic and lithophytic orchids in the family Orchidaceae, also have α-glucosidase and lipase activity and can also be used for the treatment of diabetes [52]. Catechins and catechin gallates have been found in carao and have been reported to inhibit activity against maltase. Polyphenols have been reported to have enzymatic inhibitory actions toward lipase and glucosidase activities [53].
their antioxidant contents and bioactive compounds such as flavonoids [50,51]. Free phenolics of a peanut meal have been shown to inhibit α-glucosidase and pancreatic lipase [48]. Other plants such as Dendrobium, genus of mostly epiphytic and lithophytic orchids in the family Orchidaceae, also have α-glucosidase and lipase activity and can also be used for the treatment of diabetes [52]. Catechins and catechin gallates have been found in carao and have been reported to inhibit activity against maltase. Polyphenols have been reported to have enzymatic inhibitory actions toward lipase and glucosidase activities [53].

Transepithelial Electrical Resistance and Paracellular Permeability
Transepithelial electrical resistance (TEER) was applied to measure cell monolayer integrity. The results are shown in Figure 3. All YS and control yogurt changed the function of Caco-2 cells treated with an inflammatory stimulus. The inflammatory stimulus mixture induced a 52.71% decrease in TEER value in the inflammation-induced sample. Decrease in TEER value in the sample with the inflammatory stimulus (interleukin-1β, tumor necrosis factor-α, interferon-gamma, LPS, and isoflavone genistein) (242.73 Ω·cm 2 ) happened due to disruptions to barrier integrity after 72 h (Figure 3a). Consequently,

Transepithelial Electrical Resistance and Paracellular Permeability
Transepithelial electrical resistance (TEER) was applied to measure cell monolayer integrity. The results are shown in Figure 3. All YS and control yogurt changed the function of Caco-2 cells treated with an inflammatory stimulus. The inflammatory stimulus mixture induced a 52.71% decrease in TEER value in the inflammation-induced sample. Decrease in TEER value in the sample with the inflammatory stimulus (interleukin-1β, tumor necrosis factor-α, interferon-gamma, LPS, and isoflavone genistein) (242.73 Ω·cm 2 ) happened due to disruptions to barrier integrity after 72 h (Figure 3a). Consequently, intestinal protection dysfunction was induced in Caco-2 cells by applying the inflammatory stimulus. Yogurt samples did not affect the TEER values in the first 24 h, but the TEER significantly (p > 0.05) increased after 48 h. The 5.3 g/L CY had a higher effect on TEER value up to 506.76 Ω·cm 2 , compared to 1.3 and 2.65 g/L CY. Flavonoid and phenolic compounds of carao fruit could inhibit an induced barrier dysfunction after 24 h. There was no significant difference (p < 0.05) between TEER of control and 1.2, 2.65 5.3 g/L CY after 72 h.
intestinal protection dysfunction was induced in Caco-2 cells by applying the inflammatory stimulus. Yogurt samples did not affect the TEER values in the first 24 h, but the TEER significantly (p > 0.05) increased after 48 h. The 5.3 g/L CY had a higher effect on TEER value up to 506.76 Ω·cm 2 , compared to 1.3 and 2.65 g/L CY. Flavonoid and phenolic compounds of carao fruit could inhibit an induced barrier dysfunction after 24 h. There was no significant difference (p < 0.05) between TEER of control and 1.2, 2.65 5.3 g/L CY after 72 h. Δ TEER (differential) of yogurt with 100 µM genistein was -526.17 Ω·cm 2 (Figure 3b), indicating that it significantly (p > 0.05) decreased TEER values when compared to IS samples having less inflammation. According to the results, control and CY could decrease the inflammation in Caco-2 cells while the most effective treatment among the others was 5.3 g/L CY (−567.36 Δ TEER Ω·cm 2 ). An increase in the permeability of fluorescein isothiocyanate dextran 4000 (FD, Figure 4a, and LY, Figure 4b; ug/mL) and a decrease in TEER ∆ TEER (differential) of yogurt with 100 µM genistein was -526.17 Ω·cm 2 (Figure 3b), indicating that it significantly (p > 0.05) decreased TEER values when compared to IS samples having less inflammation. According to the results, control and CY could decrease the inflammation in Caco-2 cells while the most effective treatment among the others was 5.3 g/L CY (−567.36 ∆ TEER Ω·cm 2 ). An increase in the permeability of fluorescein isothiocyanate dextran 4000 (FD, Figure 4a, and LY, Figure 4b; ug/mL) and a decrease in TEER (Figure 2) by cells treated with only interleukin-1β, tumor necrosis factor-α, interferon-gamma, LPS, and isoflavone genistein illustrates that the inflammatory stimulus damaged the integrity of the Caco-2 cell monolayer [54]. Tight junction proteins, occludin, and (ZO)-1 decrease resulted from a disruption of the intestinal barrier, which was induced by inflammatory stimulus [54]. It is likely that a change in the mRNA expression of proteins caused the barrier function improvement by adding yogurt and carao powder [36]. In relation to this, rich polyphenolic ingredients are desirable for the fortification of cultured dairy products to improve the products' functionality. Polyphenols are known to moderate the overexpression of adhesion molecules (VCAM-1 and ICAM-1) by inhibiting the NF-κB pathway in endothelial cells [55].
( Figure 2) by cells treated with only interleukin-1β, tumor necrosis factor-α, interferongamma, LPS, and isoflavone genistein illustrates that the inflammatory stimulus damaged the integrity of the Caco-2 cell monolayer [54]. Tight junction proteins, occludin, and (ZO)-1 decrease resulted from a disruption of the intestinal barrier, which was induced by inflammatory stimulus [54]. It is likely that a change in the mRNA expression of proteins caused the barrier function improvement by adding yogurt and carao powder [36]. In relation to this, rich polyphenolic ingredients are desirable for the fortification of cultured dairy products to improve the products' functionality. Polyphenols are known to moderate the overexpression of adhesion molecules (VCAM-1 and ICAM-1) by inhibiting the NF-κB pathway in endothelial cells [55].

Transmission Electron Microscopy
Transmission electron microscopy (TEM) images are shown in Figure 5, illustrating the effect of carao powder in Caco-2 cell monolayer tight junctions' integrity. The presence of electron-dense material (black streaks or electron-dense areas in Figure 5F) in the space between cells near the brush border reflects the tight junctions [37]. In cells treated with only the inflammatory stimulus ( Figure 5A), the tight junctions, as indicated by the area of black streaks, were less when compared to cells treated with control cells (healthy cells) ( Figure 5F), control yogurt ( Figure 5B), and CY ( Figure 5C-E). CY ( Figure 5C-E) contained more tight junctions (electron-dense material or black streaks) when compared to cells treated with control yogurt ( Figure 5B). In TEM images, the tight junctions in the 5.3 g/L of CY samples ( Figure 5E) were larger than the other YS ( Figure 5B-E). The TEM images are related to the TEER and paracellular permeability measurements, where 5.3 g/L of CY reduces inflammation when compared to other the treatments by showing the integrity of the tight junctions. The increased tight junctions' intact structure from CY samples could possibly be due to the protein content and phytochemicals in the carao [56].

Transmission Electron Microscopy
Transmission electron microscopy (TEM) images are shown in Figure 5, illustrating the effect of carao powder in Caco-2 cell monolayer tight junctions' integrity. The presence of electron-dense material (black streaks or electron-dense areas in Figure 5F) in the space between cells near the brush border reflects the tight junctions [37]. In cells treated with only the inflammatory stimulus ( Figure 5A), the tight junctions, as indicated by the area of black streaks, were less when compared to cells treated with control cells (healthy cells) ( Figure 5F), control yogurt ( Figure 5B), and CY ( Figure 5C-E). CY ( Figure 5C-E) contained more tight junctions (electron-dense material or black streaks) when compared to cells treated with control yogurt ( Figure 5B). In TEM images, the tight junctions in the 5.3 g/L of CY samples ( Figure 5E) were larger than the other YS ( Figure 5B-E). The TEM images are related to the TEER and paracellular permeability measurements, where 5.3 g/L of CY reduces inflammation when compared to other the treatments by showing the integrity of the tight junctions. The increased tight junctions' intact structure from CY samples could possibly be due to the protein content and phytochemicals in the carao [56].

Immunofluorescence Microscopy
For localizing ZO-1 (Figure 6), occludin-1 (Figure 7), and claudin-1 (Figure 8) in fully differentiated Caco-2 cells, different treatments and control were observed with immunofluorescence microscopy. ZO-1 is dispersed vertically throughout the cytoplasm. The presence of net-green patterns between cells for ZO-1 were detected on the immunofluorescence microscopy photos which confirmed that the cells were not merged, showing fully func-tional tight junctions. The presence of net-green patterns between cells (red arrows in Figure 6F) in the space between cells near the brush border reflects the ZO-1 tight junction's intact structure, similar to previous studies [34,37]. On the other hand, in cells with the inflammatory stimulus, the ZO-1 green patterns appear less preserved (Figure 6A), and a significantly (p > 0.05) lower florescence intensity (Figure 9) was observed when compared to cells-treated YS (Figure 6B-E) and control cells (healthy cells) ( Figure 6F). Similarly, occludin-1 ( Figure 7A-F), which is found at the lateral apical end of the cytoplasm, showed a similar trend to ZO-1. The claudin-1 and occludin-1 relative intensity/cell were improved by 5.3 g/L carao yogurt having a higher florescence intensity when compared to control cells (Figure 9b,c).

Immunofluorescence Microscopy
For localizing ZO-1 (Figure 6), occludin-1 (Figure 7), and claudin-1 (Figure 8) in fully differentiated Caco-2 cells, different treatments and control were observed with immunofluorescence microscopy. ZO-1 is dispersed vertically throughout the cytoplasm. The presence of net-green patterns between cells for ZO-1 were detected on the immunofluorescence microscopy photos which confirmed that the cells were not merged, showing fully functional tight junctions. The presence of net-green patterns between cells (red arrows in Figure 6F) in the space between cells near the brush border reflects the ZO-1 tight junction's intact structure, similar to previous studies [34,37]. On the other hand, in cells with the inflammatory stimulus, the ZO-1 green patterns appear less preserved ( Figure  6A), and a significantly (p > 0.05) lower florescence intensity (Figure 9) was observed when compared to cells-treated YS (Figure 6B-E) and control cells (healthy cells) ( Figure 6F). Similarly, occludin-1 ( Figure 7A-F), which is found at the lateral apical end of the cytoplasm, showed a similar trend to ZO-1. The claudin-1 and occludin-1 relative intensity/cell were improved by 5.3 g/L carao yogurt having a higher florescence intensity when compared to control cells (Figure 9b,c).         On the other hand, the claudin-1 ( Figure 8A-F) was distributed tightly through the cell and it was found to have a higher florescence intensity in 5.3 g/L CY samples ( Figure  8E) (Figure 9c) when compared to the control yogurt ( Figure 8B) and control cells (healthy cells) ( Figure 8F). Similar to occludin-1 and ZO-1, cells treated with the inflammatory stimulus decreased the staining of claudin-1 tight junction proteins [34], where fewer netgreen patterns were observed when compared to cells-treated YS ( Figure 8B-E). Figures On the other hand, the claudin-1 ( Figure 8A-F) was distributed tightly through the cell and it was found to have a higher florescence intensity in 5.3 g/L CY samples ( Figure 8E) (Figure 9c) when compared to the control yogurt ( Figure 8B) and control cells (healthy cells) ( Figure 8F). Similar to occludin-1 and ZO-1, cells treated with the inflammatory stimulus decreased the staining of claudin-1 tight junction proteins [34], where fewer net-green patterns were observed when compared to cells-treated YS ( Figure 8B-E). Figures 6-8 confirm how carao powder impacts the functional tight junctions with steady mesh-like patterns among adjoining cells, demonstrating its localization and integrity. Different studies have tested the effectiveness of various probiotic bacteria in improving tight junctions, including Lactobacillus rhamnosus GG, Lactobacillus acidophilus, Lactobacillus Plantarum, Bifidobacterium infantis, Bifidobacterium animalis lactis BB-12, and Escherichia coli Nissle 1917 [57]. The immunofluorescence microscopy observations confirmed the observations of transmission electron microscopy ( Figure 5), where cells treated with YS improved the tight junctions.
There are very few studies regarding the effects of yogurt on gut inflammation. The consumption of whole and skimmed yogurt has been associated with an anti-inflammatory effect in subjects with metabolism disorders, including obesity and overweight [58]. The study by Meng et al. (2017) [59] shows the disparity in its effects on markers of inflammation since the consumption of yogurt improves some markers, such as TLR-2 in monocytic cells (CD4 + HLA − DR+). Nevertheless, it does not modify the levels of TNF-α or IL-6 in peripheral blood mononuclear cells (PBMC) stimulated in vitro. In contrast, the study by Meyer et al. (2007) [60] suggests a possible role for yogurt in the stimulation of the immune system against infections, since an increase in TNF-α and IL-1β levels in PBMC stimulated in vitro with LPS or phytohemagglutinin (PHA), while the study by Pei et al. (2017) [61] shows a reduction in the levels TNF-α plasma levels and an enhancement of endotoxemia markers (LPS binding protein (LBP)/sCD14) compared to the control group, without affecting the levels of IL-6 and protein C reagent (PCR).

Gene Expression Analysis of Tight Junction Proteins
The real-time quantitative polymerase chain reaction was applied to examine if yogurt fortified with carao improved the gene expression of tight junction proteins occludin, ZO-1, and claudin-1 in Caco-2 cells due to their significance in modulating intestinal barrier functions. The relative expression of occludin, ZO-1, and claudin-1 is shown in Figure 10. When Caco-2 cells were treated with only inflammatory stimulus (IL-1β), (TNF-α (IFN-γ), (LPS), and isoflavone genistein), the relative expression of occludin and ZO-1 decreased when compared to control (cells with no inflammatory stimulus). The levels of the relative expression of ZO-1 for YS did not differ when compared to the control. On the other hand, the levels of relative expression of occludin and claudin-1 increased with yogurt fortified with 2.65 g/L and 5.3 g/L CY when compared to control samples. The most notable increase in claudin-1 gene relative expression was observed when cells were treated with 5.3 g/L CY. Previous studies have also found that occludin and ZO-1 gene relative expressions do not change noticeably when they are damaged due to inflammation [62]. The increase in the relative expression of occludin and claudin-1 in yogurt fortified with 2.65 g/L and 5.3 g/L carao and the inflammatory stimulus were related to the observations of transmission electron and immunofluorescence microscopy ( Figures 5-8). Furthermore, the paracellular permeability and transepithelial electrical resistance show that yogurt fortified with carao decreases intestinal barrier dysfunction by improving the tight junction proteins.

Fasting Blood Glucose and Oral Glucose Tolerance
Normal plasma glucose concentration is represented in Table 2. Fasting blood glucose varied from 4.02 ± 0.45 mmol/L at day zero to 6.33 ± 0.78 mmol/L at 42 days in healthy rats. The diabetic group had the highest blood sugar (15.13 ± 1.35 mmol/L) after 42 days. Blood sugar increase results in alloxan's toxic effect, which reduces glucose-induced insu-

Fasting Blood Glucose and Oral Glucose Tolerance
Normal plasma glucose concentration is represented in Table 2. Fasting blood glucose varied from 4.02 ± 0.45 mmol/L at day zero to 6.33 ± 0.78 mmol/L at 42 days in healthy rats. The diabetic group had the highest blood sugar (15.13 ± 1.35 mmol/L) after 42 days. Blood sugar increase results in alloxan's toxic effect, which reduces glucose-induced insulin secretion via glucokinase activity inhibition, resulting in insulin-dependent diabetes and beta cell necrosis [63]. The metformin group, which decreases the intestinal absorption of glucose and hepatic glucose production, had the highest effect on the decrease in blood sugar and showed a significant difference (p < 0.05) from the control group and CY samples. Among the control yogurt and CY samples, the most effective treatment was 5.3 g/L yogurt, showing the highest reduction in blood sugar after 42 days of feeding. Al-Salami et al. (2008) [64] reported that probiotic products had no effects on the blood sugar of healthy rats while having a significant effect on diabetic rats' blood sugar due to an increase in gliclazide bioavailability, which could improve glucokinase activity. There are other fruit extracts such as monk fruit that, when added to yogurt, are used as natural sweeteners and have positive effects on glucose regulation in type 2 DM [65]. The possible mechanisms of action could be associated with the β-cell function integrity, enhanced gut microbiota, and decreased insulin resistance. Other works such as the inclusion of honey in yogurt improved type 2 diabetes [66]. An analysis of AUC (area under the curve for glucose tolerance over time) indicates significant differences (p < 0.05) between treatments and control (healthy rats) (Figure 11a). Among yogurt samples, 2.65 g/L and 5.3 g/L had the higher values, leading to conclude that the glycemic response of the yogurt sample groups significantly improved with carao fortification [67].

Conclusions
Intestinal barrier dysfunction is associated with inflammatory bowel disease, obesity, and leaky gut syndrome. Overall, results indicated that yogurt fortified with carao had a positive effect on pH, apparent viscosity, total flavonoid content, TEER (Ω·cm 2 ), LY and FD (ug/mL), α-glucosidase activity, and lipase inhibition activity, fasting blood sugar (Mmol/L), and AUC (Mmol/L). Functional effects of the formulation of yogurt supplemented with carao were also evaluated in terms of the antioxidant activity, the α- The oral glucose tolerance test values are shown in Figure 11b. Glucose concentration for carao yogurt at 5.3 g/L dramatically decrease at 30 min and then slowly decreased to the initial level, whereas the diabetic group increased up to 30 Mmol/L at 120 min (Figure 11b). Yogurt has shown to normalize the glucose levels in high-fat diet-induced metabolic syndrome and oxidative stress of the obese rats to prevent glucose fluctuation [68]. In some studies, carao has shown great antidiabetic potential in animal models and improves the attributes of probiotic bacteria such as L. acidophilus [69,70]. For this reason, it is recommended for fortification purposes in cultured dairy products when trying to develop a product for consumers with leaky gut and diabetic complications.

Conclusions
Intestinal barrier dysfunction is associated with inflammatory bowel disease, obesity, and leaky gut syndrome. Overall, results indicated that yogurt fortified with carao had a positive effect on pH, apparent viscosity, total flavonoid content, TEER (Ω·cm 2 ), LY and FD (ug/mL), α-glucosidase activity, and lipase inhibition activity, fasting blood sugar (Mmol/L), and AUC (Mmol/L). Functional effects of the formulation of yogurt supplemented with carao were also evaluated in terms of the antioxidant activity, the α-glycosidase activity, and lipase inhibitory properties. In addition, the hypoglycemic potential was in vivo validated in a rat model. Compared to the control yogurt, Caco-2 TEER (transepithelial electrical resistance evaluation) by yogurt with 5.3 g/L of carao was significantly lower (p < 0.05) after 48 h. Yogurt with 5.3 g/L of carao had a considerably lower permeability (p < 0.05) than control yogurt in FD and LY flux. The main reason for these effects might be due to the flavonoid and bioactive content of the carao pulp, which improved the characteristics of yogurt compared to the control yogurt sample. To sum up, yogurt fortified with carao improves the integrity of Caco-2 cells by strengthening tight junctions and has a predominant hypoglycemic effect in rats. This study suggests fortifying yogurt with carao when improving the functionality of yogurt in terms of intestinal barrier functions and antidiabetic potential.  Institutional Review Board Statement: The established animal experiment guidelines by the Declaration of Helsinki were used as a reference for the animal models, which is approved by the Ethics Committee in Food Research at the Honduran Association of Medicine and Nutrition (ASOHMENU) with form number AS-ASHOMENU-0013-2022.

Informed Consent Statement: Not applicable.
Data Availability Statement: The authors confirm that the data supporting the findings of this studyare available within the article and the raw data that support the findings are available from thecorresponding author, upon reasonable request. (PDF) Attributes of Lactobacillus acidophilus as Effected by Carao (Cassia grandis) Pulp Powder. Available from: https://www.researchgate.net/publication /370221110_Attributes_of_Lactobacillus_acidophilus_as_Effected_by_Carao_Cassia_grandis_Pulp_ Powder (accessed 7 June 2023).