Determination of Foam Stability in Lager Beers Using Digital Image Analysis of Images Obtained Using RGB and 3D Cameras

: Foam stability and retention is an important indicator of beer quality and freshness. A full, white head of foam with nicely distributed small bubbles of CO 2 is appealing to the consumers and the crown of the production process. However, raw materials, production process, packaging, transportation, and storage have a big impact on foam stability, which marks foam stability moni-toring during all these stages, from production to consumer, as very important. Beer foam stability is expressed as a change of foam height over a certain period. This research aimed to monitor the foam stability of lager beers using image analysis methods on two different types of recordings: RGB and depth videos. Sixteen different commercially available lager beers were subjected to analysis. The automated image analysis method based only on the analysis of RGB video images proved to be inapplicable in real conditions due to problems such as reﬂection of light through glass, autofocus, and beer lacing/clinging, which make it impossible to accurately detect the actual height of the foam. A solution to this problem, representing a unique contribution, was found by introducing the use of a 3D camera in estimating foam stability. According to the results, automated analysis of depth images obtained from a 3D camera proved to be a suitable, objective, repeatable, reliable, and sufﬁciently sensitive method for measuring foam stability of lager beers. The applied model proved to be suitable for predicting changes in foam retention of lager beers.


Introduction
Ancient beer displayed weak or no foam. Uncontrolled fermentation, no addition of hops, and subsequent low carbonation resulted in a foam-less beverage. Research conducted by [1] on ancient Finnish beer Sahti showed that the investigated ancient beverage had no foam and showed a distinctive difference between today's beers, especially regarding flavor and aroma. Today's brewing industries are far from the ancient manufacturers, and stable and retentive foam head is one of the main indicators of beer freshness and quality. Even though a big and rich head of foam is a property of certain types of beer (lager, pilsner, and wheat beer among others), every consumer seeks freshness in a preferable label. Some people do not appreciate foam in their glass, regardless of the beer style, some love the lacy pattern at the bottom of the finished beer, but the majority of beer-lovers like the crystal-clear glass after finishing the last sip [2]. Cling can be described as the adhesion of beer foam to the side of the glass during beer consumption, commonly known as "lacing". For example, Belgium is known for beers that leave a lacy glass. According to BJCP Beer Style Guidelines [3], "Belgian Lace is a characteristic and persistent latticework pattern of foam left on the inside of the glass as a beer is consumed. The look is reminiscent of fine lacework from Brussels or Belgium, and is a desirable indicator of beer quality Analysis Commission) procedure (method 2.23.1) [27], at an angle of 135°. The pourer took extra care to pour every sample evenly and uniformly into the glass (Figure 1). When the pouring was done, the sample was placed on a designated spot, and cameras were set to measure the foam stability. According to MEBAK, foam in lager beers should be stable for 3-5 min.
Two vision-based approaches to measuring foam stability were implemented: image analysis of RGB video and depth measurement using a 3D camera. Over a period of 5 min, a visual RGB camera (Canon PowerShot G16; Ota City, Tokyo, Japan) was used to take a video recording of the beer. Simultaneously, a 3D camera (Orbbec Astra S; Orbbec 3D Technology International, Inc., Troy, MI, USA) was also used to take depth measurements. Figure 1 shows the experimental setup used in data collection.

Image Analysis of Video
A visual RGB camera (Canon G16) was used to take a video recording of each sample over a period of 5 min. The recorded video had a frame rate of 30 fps. Image analysis was performed on the recorded video. The image analysis procedure to determine the height of beer foam is explained in the following seven steps below: Step 1: For a given frame of the recorded video, define a region of interest (ROI) of known width (w) and height (l) in the image that contains beer and foam, as shown in Figure 2a. It is important that the ROI covers the whole height of the foam and part of the beer. All the following image processing steps are performed on this ROI. This ensures that all the image processing steps are directed or focused on segmenting the foam head from the rest of the image within the ROI.
Step 2: Perform color segmentation by filtering (thresholding) the ROI in HSV color space by defining the lower and upper values of the color of the foam (Figure 2b). HSV color space separates color information (chroma) from intensity (luma). Since the value is separated, thresholding can theoretically be performed using only saturation and hue. More robust color thresholding over simpler parameters can be performed in HSV color space than in RGB color space. For the purposes of the results presented in this paper, the lower HSV boundary of (0,0,230)was used, while the upper boundary was defined as (43, 18,255) Step 3: Generate a binary image of the thresholded ROI in HSV color space ( Figures  2c and 3a  Two vision-based approaches to measuring foam stability were implemented: image analysis of RGB video and depth measurement using a 3D camera. Over a period of 5 min, a visual RGB camera (Canon PowerShot G16; Ota City, Tokyo, Japan) was used to take a video recording of the beer. Simultaneously, a 3D camera (Orbbec Astra S; Orbbec 3D Technology International, Inc., Troy, MI, USA) was also used to take depth measurements. Figure 1 shows the experimental setup used in data collection.

Image Analysis of Video
A visual RGB camera (Canon G16) was used to take a video recording of each sample over a period of 5 min. The recorded video had a frame rate of 30 fps. Image analysis was performed on the recorded video. The image analysis procedure to determine the height of beer foam is explained in the following seven steps below: Step 1: For a given frame of the recorded video, define a region of interest (ROI) of known width (w) and height (l) in the image that contains beer and foam, as shown in Figure 2a. It is important that the ROI covers the whole height of the foam and part of the beer. All the following image processing steps are performed on this ROI. This ensures that all the image processing steps are directed or focused on segmenting the foam head from the rest of the image within the ROI.
Step 2: Perform color segmentation by filtering (thresholding) the ROI in HSV color space by defining the lower and upper values of the color of the foam (Figure 2b). HSV color space separates color information (chroma) from intensity (luma). Since the value is separated, thresholding can theoretically be performed using only saturation and hue. More robust color thresholding over simpler parameters can be performed in HSV color space than in RGB color space. For the purposes of the results presented in this paper, the lower HSV boundary of (0,0,230)was used, while the upper boundary was defined as (43,18,255).
Step 3: Generate a binary image of the thresholded ROI in HSV color space (Figures 2c  and 3a), i.e., all pixels that have values within the defined boundaries are marked as white pixels (values are set to 255), while the remaining pixels are marked as black (values are set to 0).
Step 4: Perform morphological operations of erosion followed by dilation on the binary image (Figures 2d and 3b). These operations are needed in order to eliminate small white noises or white artifacts that appear in the binary image. Step 5: Determine the largest contour from the list of all contours on the binary image ( Figure 2e). A contour is a curve joining all the continuous points or connected components (along the boundary) having the same color or intensity. This step basically segments or marks the boundary of the foam/head.
Step 6: Determine the area (A) of the region defined by the largest contour.
Step 7: The average height (h) of the beer foam in pixels can be determined using Equation (1): Using the notation where h t represents the height of foam (in pixels) at a given point in time t (in seconds), the maximum height, h max , is defined as: and the minimum height, h min , is defined as Based on these definitions, we define the normalized foam height at time t, h t_norm , as: Since the beer glass is always located in the same position, the seven steps provided above can be implemented as a program to automate the procedure. One advantage of this procedure is that it can be run in both offline and online mode. For the purposes of this paper, a script written in the Python programming language [28] using the OpenCV library [29] was implemented in order to automate the process of determining the beer foam height from the recorded videos. Every 10 s, five consecutive frames were taken and the height of foam determined for each frame. The average height in pixels for these five measurements was taken to represent the height of foam every 10 s. Step 4: Perform morphological operations of erosion followed by dilation on the binary image (Figures 2d and 3b). These operations are needed in order to eliminate small white noises or white artifacts that appear in the binary image.
Step 5: Determine the largest contour from the list of all contours on the binary image ( Figure 2e). A contour is a curve joining all the continuous points or connected components (along the boundary) having the same color or intensity. This step basically segments or marks the boundary of the foam/head.
Step 6: Determine the area (A) of the region defined by the largest contour.
Step 7: The average height (h) of the beer foam in pixels can be determined using Equation (1): (1) Using the notation where ht represents the height of foam (in pixels) at a given point in time t (in seconds), the maximum height, hmax, is defined as: and the minimum height, hmin, is defined as Based on these definitions, we define the normalized foam height at time t, ht_norm, as:  Figure 3a); (d) morphological operations of erosion followed by dilation performed on binary image to remove artifacts (a magnified image is provided in Figure 3b); (e) largest contour found marked in red. The estimated height of foam in pixels is determined using Equation (1).  Figure 3a); (d) morphological operations of erosion followed by dilation performed on binary image to remove artifacts (a magnified image is provided in Figure 3b); (e) largest contour found marked in red. The estimated height of foam in pixels is determined using Equation (1).  Since the beer glass is always located in the same position, the seven steps provided above can be implemented as a program to automate the procedure. One advantage of this procedure is that it can be run in both offline and online mode. For the purposes of this paper, a script written in the Python programming language [28] using the OpenCV library [29] was implemented in order to automate the process of determining the beer foam height from the recorded videos. Every 10 s, five consecutive frames were taken and the height of foam determined for each frame. The average height in pixels for these five measurements was taken to represent the height of foam every 10 s.

Depth Measurement Using a 3D Camera
A 3D camera provides a depth map or a depth image where each pixel in the image relates to the distance between the surface of the object being viewed and the camera or image plane. The Orbbec Astra S 3D camera used in this paper is based on the Structured-Light technology. The 3D camera consists of an infrared laser projector and a proprietary Infra-Red (IR) depth sensor. The depth sensor interprets 3D scene information based on continuously projected infrared structured light. It should also be mentioned that Orbbec Astra S 3D camera also consists of an RGB camera. However, this RGB camera was not used in the experiments for the purpose of this paper. An example of a depth image generated by the 3D camera is shown in Figure 4.
Orbbec Astra S depth sensor has a camera resolution of 640 × 480 and a maximum frame rate of 30 Hz. Its measurement range is from 0.4 to 2 m and has a field of view of 60° horizontally and 49.5° vertically. It also has an accuracy of +/−1-3 mm at 1 m.
One drawback of the sensor is that it cannot detect glass nor liquids, so for example, in Figure 4, it can be seen that the edge of the beer glass cannot be detected (pixels displayed in black), and when the foam disappears no depth information can be obtained.
As displayed in Figure 1, the 3D camera was placed above the beer glass. Hence, all measurements obtained from the 3D camera actually provided the distance of the top of the foam head from the 3D camera. Thus, measurements of the distance of the foam for a given sample increased with time, as the foam in the glass decreased.
Similar to the measurements performed when using the video camera in the previous section, since the beer glass was always located in the same position, a fixed ROI was defined (Figure 4a), and a Python script was used in order to automate the process of determining the distance of the beer foam height from the camera. Every 10 s, five consecutive frames were taken, and the average distance of foam from the camera was determined for each frame. This average distance was determined using only the pixels within the defined ROI having a depth value. Pixels without depth values were excluded. The

Depth Measurement Using a 3D Camera
A 3D camera provides a depth map or a depth image where each pixel in the image relates to the distance between the surface of the object being viewed and the camera or image plane. The Orbbec Astra S 3D camera used in this paper is based on the Structured-Light technology. The 3D camera consists of an infrared laser projector and a proprietary Infra-Red (IR) depth sensor. The depth sensor interprets 3D scene information based on continuously projected infrared structured light. It should also be mentioned that Orbbec Astra S 3D camera also consists of an RGB camera. However, this RGB camera was not used in the experiments for the purpose of this paper. An example of a depth image generated by the 3D camera is shown in Figure 4.
FOR PEER REVIEW 6 of 13 mean distance of these average distances for the five measurements was taken to represent the distance of foam from the camera every 10 s. If dt represents the distance of foam (in mm) from the camera at a given point in time (t = 0, 1, …,300 s), and dtable represents the distance of the table (in mm) from the camera (dtable = 654 mm. Figure 1.), the height of the foam from the top of the table at time t, difft, is given by The maximum height of the foam from the top of the table, diffmax, is defined as: and the minimum height, diffmin, is defined as Based on these definitions, the normalized foam height at time t, ht_norm, is given by: It is important to emphasize that the images displayed in Figure 4 represent depth images. Areas in the images having shades of gray have depth information, while those marked in black do not. The edge of the beer glass cannot be detected in Figure 4a due to the fact that (a) the sensor cannot detect glass, since the transmitted light is not reflected, and (b) the non-defined area near the beer glass is also extended as a result of parallax, since the emitter and the sensor on the 3D camera are separated by about 7.5 cm. In Figure  4b, after the foam disappears, the transmitted beam of the 3D sensor cannot be reflected Orbbec Astra S depth sensor has a camera resolution of 640 × 480 and a maximum frame rate of 30 Hz. Its measurement range is from 0.4 to 2 m and has a field of view of 60 • horizontally and 49.5 • vertically. It also has an accuracy of +/−1-3 mm at 1 m. One drawback of the sensor is that it cannot detect glass nor liquids, so for example, in Figure 4, it can be seen that the edge of the beer glass cannot be detected (pixels displayed in black), and when the foam disappears no depth information can be obtained.
As displayed in Figure 1, the 3D camera was placed above the beer glass. Hence, all measurements obtained from the 3D camera actually provided the distance of the top of the foam head from the 3D camera. Thus, measurements of the distance of the foam for a given sample increased with time, as the foam in the glass decreased.
Similar to the measurements performed when using the video camera in the previous section, since the beer glass was always located in the same position, a fixed ROI was defined (Figure 4a), and a Python script was used in order to automate the process of determining the distance of the beer foam height from the camera. Every 10 s, five consecutive frames were taken, and the average distance of foam from the camera was determined for each frame. This average distance was determined using only the pixels within the defined ROI having a depth value. Pixels without depth values were excluded. The mean distance of these average distances for the five measurements was taken to represent the distance of foam from the camera every 10 s.
If d t represents the distance of foam (in mm) from the camera at a given point in time (t = 0, 1, . . . ,300 s), and d table represents the distance of the table (in mm) from the camera (d table = 654 mm. Figure 1.), the height of the foam from the top of the table at time t, diff t , is given by The maximum height of the foam from the top of the table, diff max , is defined as: and the minimum height, diff min , is defined as diff min = min {diff t : t = 0, 1, . . . ,300}.
Based on these definitions, the normalized foam height at time t, h t_norm , is given by: It is important to emphasize that the images displayed in Figure 4 represent depth images. Areas in the images having shades of gray have depth information, while those marked in black do not. The edge of the beer glass cannot be detected in Figure 4a due to the fact that (a) the sensor cannot detect glass, since the transmitted light is not reflected, and (b) the non-defined area near the beer glass is also extended as a result of parallax, since the emitter and the sensor on the 3D camera are separated by about 7.5 cm. In Figure 4b, after the foam disappears, the transmitted beam of the 3D sensor cannot be reflected by the beer surface, and therefore it is impossible to detect the depth of the beer surface. This criterion was used for ending measurements in situations when the foam disappeared before 5 min.

Time (s)
ation 2021, 7, x FOR PEER REVIEW 8 of 13 bubble (disproportionation), so the more gas there is in foam, the greater the disproportionation, which was the case for most samples, but sample s02 was particularly erratic. If the gas fraction in liquid (beer) foams is high, the bubbles cannot form exclusively spheres, but they take forms of polyhedra separated by thin layers of the liquid phase called lamellae. Another important phenomenon is that hydrophobic particles adsorbed at the gasliquid interface tend to compress together as bubbles contract to form barriers that prevent the continuation of disproportionation. At constant pressure, the size of bubbles is directly proportional to the surface tension. Thus, materials with lower surface tension also give smaller bubbles [2]. Coalescence or merging of two bubbles occurs upon rupture of the membrane that divides them. This leads to coarsening of foam with visible larger bubblesfish eyes in the foam body [4]. The Young-Laplace equation describes the disproportionation as the differential pressures between the inside and outside of a bubble due to surface tension. This pressure is inversely proportional to the bubble radius, causing CO2 gas to diffuse from smaller bubbles where pressure is higher into larger bubbles. According to Hackbath [4], "as the foam structure coarsens and larger bubbles continue to expand, their membranes thin until they reach a critical thickness. Film ruptures can be spontaneous or can be caused by fats that interfere with the film's external surface. Collapse occurs at the crown surface by rupture or by diffusion of dipolar CO2 directly to the atmosphere through the CO2 permeable bubble film". Comprehension of all stated data could explain the behavior of foam in sample s02. It can be presumed that this is due to the storage in unsuitable conditions in the supermarket storage space. All samples were purchased in January, when it was cold in the storage rooms of the market place, and all analyses were done in January. The temperature fluctuations in the storage room, where it is cooler, then sudden transfer to higher temperatures at the market place could cause this kind of foaming properties loss in most of the samples. As for the sample s02, it could be some type of production error in this particular batch. Apart from sample s02, samples s16 and s07 seemed to show a more stable foam in comparison to all the other samples. It appears that this foam showed significantly more stable properties, even though all samples were kept at the same temperature. This hypothesis has yet to be confirmed by detailed laboratory testing, although preliminary analysis of new samples obtained in March (which show normal behavior) lead us to this conclusion.    s04  s05  s06  s07  s08  s09  s10  s11  s12  s13  s14  s15  s16   0  158 159 161 172  171  177  181  176  178  167  177  170  134  168  165  191  10  41 158 160 169  167  169  177  171  174  166  175  162  36  166  163  189  20  -158 152 157  156  157  173  163  169  154  165  156  -163  160  184  30  -158  -153  148  152  169  156  165  145  155  --161  158  181  40  -159  --146  -166  152  161  137  149  --159  157  177  50  -159  --145  -163  150  157  134  147  --157  156  174  60  -159  ----161  150  153  133  145  --156  155  171  70  -159  ----158  149  150  132  ---156  155  169  80 - "-" indicates that no measurements were made since there was no foam head. All values have been rounded up. Depth measurements were also being taken simultaneously using a 3D camera. The distance of the beer foam surface (in mm) from the top of the table measured over time, difft, for the 16 beer samples is displayed in Table 2. The sign "-" indicates that measurements were not possible since there was no foam head.  s02 s03 s04 s05 s06 s07 s08 s09 s10 s11 s12 s13 s14 s15 s16  0  158 159 161 172 171 177 181 176 178 167 177 170 134 168 165   The processed results of the measurements obtained using the visual RGB camera are displayed in Table 1. Even though the results are displayed in pixels, the corresponding height of beer foam can be obtained by using the conversion of 1 mm = 6.6 px. The sign "-" in the table indicates that measurements were stopped since there was no foam.
A graphical representation of the normalized measurement results obtained by performing image analysis on RGB videos can be seen in Figure 5 (actual data is provided in Table A1). The behavior of the s02 sample is due to the increase in foam levels during the measurement as a result of erratic CO 2 bubbles that formed unstable foam, as can be seen in Figure 6. According to Bamforth [2], low surface tension is an important factor for foam formation. Constant surface tension withholds a pressure within a bubble that is inversely proportional to its diameter, so the gas makes an effort to pass from a smaller to larger bubble (disproportionation), so the more gas there is in foam, the greater the disproportionation, which was the case for most samples, but sample s02 was particularly erratic. If the gas fraction in liquid (beer) foams is high, the bubbles cannot form exclusively spheres, but they take forms of polyhedra separated by thin layers of the liquid phase called lamellae. Another important phenomenon is that hydrophobic particles adsorbed at the gas-liquid interface tend to compress together as bubbles contract to form barriers that prevent the continuation of disproportionation. At constant pressure, the size of bubbles is directly proportional to the surface tension. Thus, materials with lower surface tension also give smaller bubbles [2]. Coalescence or merging of two bubbles occurs upon rupture of the membrane that divides them. This leads to coarsening of foam with visible larger bubbles-fish eyes in the foam body [4]. The Young-Laplace equation describes the disproportionation as the differential pressures between the inside and outside of a bubble due to surface tension. This pressure is inversely proportional to the bubble radius, causing CO 2 gas to diffuse from smaller bubbles where pressure is higher into larger bubbles. According to Hackbath [4], "as the foam structure coarsens and larger bubbles continue to expand, their membranes thin until they reach a critical thickness. Film ruptures can be spontaneous or can be caused by fats that interfere with the film's external surface. Collapse occurs at the crown surface by rupture or by diffusion of dipolar CO 2 directly to the atmosphere through the CO 2 permeable bubble film". Comprehension of all stated data could explain the behavior of foam in sample s02. It can be presumed that this is due to the storage in unsuitable conditions in the supermarket storage space. All samples were purchased in January, when it was cold in the storage rooms of the market place, and all analyses were done in January. The temperature fluctuations in the storage room, where it is cooler, then sudden transfer to higher temperatures at the market place could cause this kind of foaming properties loss in most of the samples. As for the sample s02, it could be some type of production error in this particular batch. Apart from sample s02, samples s16 and s07 seemed to show a more stable foam in comparison to all the other samples. It appears that this foam showed significantly more stable properties, even though all samples were kept at the same temperature. This hypothesis has yet to be confirmed by detailed laboratory testing, although preliminary analysis of new samples obtained in March (which show normal behavior) lead us to this conclusion.
Depth measurements were also being taken simultaneously using a 3D camera. The distance of the beer foam surface (in mm) from the top of the table measured over time, diff t , for the 16 beer samples is displayed in Table 2. The sign "-" indicates that measurements were not possible since there was no foam head.
A graphical representation of the normalized distance of beer foam (%) from 3D camera can be seen in Figure 7 (actual data is provided in Table A2). Comparing Figures 5 and 6, similar conclusions about the foam stability can be made.
One major drawback of the non-invasive automated image analysis of RGB images is that it is sensitive to foam lacing or clinging. For example, s06 has a foam height of 26 px (or about 4 mm) after 40 s (see Table 1). Figure 8 shows the RGB video frame after 40 s. On the other hand, depth measurements of the foam surface by the 3D camera were not possible after 30 s, since there was no foam on the liquid surface (scenario similar to Figure 4b). This feedback (lack of depth information) from the 3D sensor was then used to stop further measurement. It should also be noted that the measurements after 10 s (see Table 2) for samples s01 and s13 should basically be ignored, since this were unreliable measurements provided by the 3D sensor in situations where there was basically no foam. A graphical representation of the normalized distance of beer foam (%) from 3D camera can be seen in Figure 7 (actual data is provided in Table A2). Comparing Figures 5 and  6, similar conclusions about the foam stability can be made. One major drawback of the non-invasive automated image analysis of RGB images is that it is sensitive to foam lacing or clinging. For example, s06 has a foam height of 26 px (or about 4 mm) after 40 s (see Table 1). Figure 8 shows the RGB video frame after 40 s. On the other hand, depth measurements of the foam surface by the 3D camera were not possible after 30 s, since there was no foam on the liquid surface (scenario similar to Figure  4b). This feedback (lack of depth information) from the 3D sensor was then used to stop further measurement. It should also be noted that the measurements after 10 s (see Table  2) for samples s01 and s13 should basically be ignored, since this were unreliable measurements provided by the 3D sensor in situations where there was basically no foam.   One major drawback of the non-invasive automated image analysis of RGB images is that it is sensitive to foam lacing or clinging. For example, s06 has a foam height of 26 px (or about 4 mm) after 40 s (see Table 1). Figure 8 shows the RGB video frame after 40 s. On the other hand, depth measurements of the foam surface by the 3D camera were not possible after 30 s, since there was no foam on the liquid surface (scenario similar to Figure  4b). This feedback (lack of depth information) from the 3D sensor was then used to stop further measurement. It should also be noted that the measurements after 10 s (see Table  2) for samples s01 and s13 should basically be ignored, since this were unreliable measurements provided by the 3D sensor in situations where there was basically no foam.

Conclusions
Beer foam stability is an important beer quality indicator. Stable beer foam after production does not have to correlate with beer foam after a certain period of storage and transport. In this research, we presented an algorithm for an automated non-invasive procedure for measuring foam height by applying image analysis of RGB images or videos. The procedure showed off as relatively robust and applicable in online and offline mode. One major drawback of this method appeared to be its sensitivity to foam lacing or cling due to poor CO 2 distribution or low foam active/stabilizing compounds concentrations (proteins or hop compounds) in beer where the camera, placed laterally in regards to the sample, could not distinguish the lacing from foam height. However, this problem was resolved by using a 3D camera, which generates depth videos or images. A 3D camera mounted directly above the glass containing the beer sample was able to measure the distance of the foam surface from the camera. By measuring the change in the distance of the foam surface from the 3D camera over time, information about the foam stability was obtained. Due to the technology used in measuring the distance of objects from the 3D camera, the camera was able to detect and recognize the foam surface, but was not able to detect the liquid surface, and therefore the distance of the liquid surface from the camera could not be measured. This usual drawback of this camera is actually an advantage in this scenario, since it provides information about the disappearance of the beer foam. Information about the lack of foam is triggered by the lack of depth information within the ROI. In any case, this could be a novel, quick, robust, precise, and accurate method for foam stability measurement.

Data Availability Statement:
The data presented in this study are available on request from the corresponding author.

Conflicts of Interest:
The authors declare no conflict of interest.