The Effects of 5% 5-Aminolevulinic Acid Gel and Red Light (ALAD-PDT) on Human Fibroblasts and Osteoblasts
Abstract
:1. Introduction
2. Results and Discussion
2.1. Cell Survival and Morphology after ALAD-PDT
2.2. Cell Proliferation after ALAD-PDT
2.3. ALAD Induces Accumulation of PpIX
2.4. Intracellular ROS Levels after ALAD-PDT
2.5. SOD Activity after ALAD-PDT
3. Conclusions
4. Materials and Methods
4.1. Experimental Design
4.2. Cell Culture and Isolation
4.3. Treatments
4.4. Lactate Dehydrogenase (LDH) Release Assay
4.5. Toluidine Blue Staining
4.6. Cell Proliferation Assay
4.7. PpIX Fluorescence
4.8. Reactive Oxygen Species (ROS) Levels
4.9. Superoxide Dismutase (SOD) Assay
4.10. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Pierfelice, T.V.; D’Amico, E.; Petrini, M.; Pandolfi, A.; D’Arcangelo, C.; Di Pietro, N.; Piattelli, A.; Iezzi, G. The Effects of 5% 5-Aminolevulinic Acid Gel and Red Light (ALAD-PDT) on Human Fibroblasts and Osteoblasts. Gels 2022, 8, 491. https://doi.org/10.3390/gels8080491
Pierfelice TV, D’Amico E, Petrini M, Pandolfi A, D’Arcangelo C, Di Pietro N, Piattelli A, Iezzi G. The Effects of 5% 5-Aminolevulinic Acid Gel and Red Light (ALAD-PDT) on Human Fibroblasts and Osteoblasts. Gels. 2022; 8(8):491. https://doi.org/10.3390/gels8080491
Chicago/Turabian StylePierfelice, Tania Vanessa, Emira D’Amico, Morena Petrini, Assunta Pandolfi, Camillo D’Arcangelo, Natalia Di Pietro, Adriano Piattelli, and Giovanna Iezzi. 2022. "The Effects of 5% 5-Aminolevulinic Acid Gel and Red Light (ALAD-PDT) on Human Fibroblasts and Osteoblasts" Gels 8, no. 8: 491. https://doi.org/10.3390/gels8080491