Macrofungi as Medicinal Resources in Uzbekistan: Biodiversity, Ethnomycology, and Ethnomedicinal Practices

Interest in edible and medicinal macrofungi is millennial in terms of their uses in health and food products in Central Asia, while interest in inedible and medicinal macrofungi has grown in popularity in recent years. Edible and inedible medicinal basidiomycetes were collected during field surveys from different regions of Uzbekistan. The morphological characters and similarity assessment of rDNA-Internal Transcribed Spacer sequence data were used to measure diversity and habitat associations. A number of 17 species of medicinal macrofungi of ethnomycological and medicinal interest was found associated with 23 species of trees and shrubs belonging to 11 families and 14 genera. Polyporaceae and Hymenochaetaceae were represented by the highest number of species followed by Ganodermataceae, Fomitopsidaceae, Auriculariaceae, Cerrenaceae, Grifolaceae, Phanerochaetaceae, Laetiporaceae, Schizophyllaceae, and Stereaceae. The highest number of medicinal basidiomycete species was reported in the following host genera: Acer, Betula, Celtis, Crataegus, Juglans, Juniperus, Lonicera, Malus, Morus, Platanus, Populus, Prunus, Quercus, and Salix. An updated list of edible and inedible medicinal mushrooms identified in Uzbekistan, their morphological characteristics, and phylogenetic placement are given for the first time. Information is provided on their uses in traditional and modern medicine. Their bioactive compounds and extracts can be applied as medicines, as well as food and cosmetic ingredients.


Introduction
Ethnomycology, an interdisciplinary field that explores the cultural and traditional uses of fungi, plays a pivotal role in documenting, presenting, and preserving the rich and diverse knowledge associated with the utilization of mushrooms and other fungal organisms in biology and ethnomedicine.Building upon this foundation, the ethnomycological study of the selected macrofungal species, including esteemed genera such as Fomes, Inonotus, Ganoderma, Phellinus, Trametes, and other macrofungal groups, has revealed their extensive utilization in health practices for millennia [1][2][3][4][5].Notably, the opulent and valuable documentation of fungal species uses in folk medicine has particularly flourished in East Asia, encompassing China, Japan, and Korea [5,6].
In addition, the aim of this study was to examine the biodiversity and systematic composition, as well as establish a modern list of medicinal mushrooms in Uzbekistan.We also intend to obtain data on the applications of particular species in both traditional and modern medical uses and to design a database on ethnomedicinal mushrooms in Uzbekistan.

Study Area
Uzbekistan, located in Central Asia extends from the foothills of the Tian Shan and Pamir mountains in the east to west of the Aral Sea.Uzbekistan borders Kazakhstan to the north, Kyrgyzstan and Tajikistan to the east and southeast, Turkmenistan to the west, and Afghanistan to the south (Figure 1).The country covers 447,400 km 2 (172,742 sq miles) and has a population of about 35 million.Uzbekistan is divided into 12 provinces and one autonomous republic with desert and mountainous areas, where continental climate mainly prevails.It is one of the most diverse regions in the world with respect to both fauna and flora; it is considered a remarkable collection of many species of medicinal plants [45,46].

Collection and Preservation of Medicinal Macrofungi Samples
This study is based on fresh basidiomata of medicinal basidiomycetes collected between 2021 and 2022 during field surveys in the provinces of Andijan, Fergana, Jizzakk, Namangan, Samarkand, Tashkent, and in the botanical garden of the city of Tashkent in Uzbekistan (Table 1).Dried specimens were used in the laboratory of mycology from the Institute of Botany to determine the taxonomic composition of species with references from various books and monographs.The collected samples were deposited at TASM (Tashkent Mycological Herbarium), the Institute of Botany of the Academy of Science of Uzbekistan after morphological study.Fungarium acronyms used in the paper are from Index Herbariorum [47].[32].

Figure 1. Map of Uzbekistan
Table 1.Description of the study sites.
five beads of 1 mm diameter per sample and shaking twice at maximum speed for 15 min.Genomic DNA was extracted using the BioSprint 96 DNA Plant Kit (QIAGEN Diagnostics GmbH, Qiagen AG, Germany) on a KingFisher Flex (Thermo Fisher Scientific, Waltham, Massachusetts, United States) robot.The amplification via PCR of the internal transcribed spacer (ITS) was performed using primers ITS1F/ITS4 or ITS1/ITS4 [48,49].Amplicons were sequenced at the Biodiversity and Climate Research Centre (BiK-F) laboratory using primers identical to those used for amplifications.
The assembled sequences obtained in this study were deposited in the NCBI GenBank (Table 2).Each sequence was compared with the reference sequences in GenBank, using a BLAST search [50].The ITS region was used to identify each species of medicinal mushrooms in Uzbekistan.Accordingly, some ITS sequences were downloaded from GenBank and incorporated with the newly generated ITS sequences in a dataset (Table 2).The ingroup taxa belong to Agaricales, Auriculariales, Hymenochaetales, Polyporales, and Russulales, while Coniophora arida and Gomphidius roseus from Boletales were selected as outgroup taxa.
MAFFT v.7.110 [51] was chosen to align the ITS region under the "G-INS-i" option [52].jModelTest v.2.1.10 [53,54] was used to estimate the best-fit evolutionary model of the alignment for phylogenetic analyses under the Akaike information criterion.Maximum Likelihood (ML) and Bayesian Inference (BI) algorithms were utilized for phylogenetic analyses.The ML algorithm was conducted using raxmlGUI v.8.2.12 [55] with the calculation of bootstrap (BS) replicates under the auto FC option [56].The BI algorithm was performed using MrBayes v.3.2.7 [57].Two independent runs were employed and each run included four chains and started from random trees.Trees were sampled every 1000th generation, and the first 25% of the sampled trees were removed, while the other 75% were retained for constructing a 50% majority consensus tree and calculating Bayesian posterior probabilities (BPPs).Chain convergence of the resulting log file was judged using Tracer v.1.7.1 [58].The final phylogenetic tree was edited and visualized by tvBOT [59].

Data Collection
We obtained distribution, habitats, taxonomy, morphology, ethnobotanical data, and ethnomedicinal uses of medicinal fungal from previously published literature, i.e., research articles, monographs, and books written in Uzbek, Russian, and English in indexed and non-indexed journals using online bibliographic databases, as well as local library sources and our own materials.In addition, scientific names of medicinal fungi and their hosts were checked for potential synonyms in Index Fungorum and Plants of the World Online [60,61].Data analysis, as well as field interviews and discussions, were conducted with forest inspectors in Uzbekistan.

Phylogenetic Placement of Collections of Medicinal Macrofungi from Uzbekistan
In this study, 24 ITS sequences were newly generated from 24 specimens (Table 2).The dataset of the ITS region generated an alignment of 930 characters with GTR + I + G as the best-fit evolutionary model.In the ML algorithm, the BS search stopped after 200 replicates.In the BI algorithm, after 15 million generations with an average standard deviation of split frequencies of 0.002282, all chains converged, which was indicated by the effective sample sizes of all parameters above 8000 and all potential scale reduction factors close to 1.000.Because ML and BI algorithms generated nearly congruent topologies, the topology from the ML algorithm is presented along with BS values and BPPs simultaneously greater than 50% and 0.8, respectively, at the nodes (Figure 2).All newly sequenced specimens grouped together with their corresponding species are represented by the sequences downloaded from GenBank with strong statistical support.This phylogeny confirmed our species morphological identification.

Distribution of Medicinal Basidiomycetes by Areas of Uzbekistan
An analysis of the distribution of medicinal basidiomycetes, most often collected in urban and mountainous areas of Tashkent province, revealed nine species (Auricularia mesenterica, Fomitopsis betulina, Fomes fomentarius, Lentinus tigrinus, Cerrena unicolor, Trametes versicolor, Phellinus igniarius, Sanghuangporus lonicerinus, and Stereum hirsutum), which constitute 52.95% of the total medicinal basidiomycete studies in this study area.The most abundant macrofungi were found in Jizzakh province, with six species records mainly distributed in Juniper forests of the Turkestan mountain range in the Zaamin district of Jizzakh (Bjerkandera adusta, Fomitopsis betulina, Laetiporus sulphureus, Phellinus igniarius, Sanghuangporus lonicerinus, and Stereum hirsutum), which constitute 35.29% of the total number of medicinal macrofungi.In the provinces of Namangan and Andijan, three species were found in each province, representing 17.64% of the total mycobiota.Macrofungal species were rare in the urban and mountain forests in Fergana, Samarkand provinces, and the Tashkent Botanical Garden where only Inonotus hispidus and Stereum hirsutum were found.Figure 3 shows the distribution of basidiomycetes in the study areas.Throughout human history, people have maintained a deep connection with macrofungi, drawing on their folk knowledge for medicinal and culinary purposes.Among these, the traditional expertise on medicinal mushrooms holds a significant and irreplaceable role in human life.It is well-documented that ancient Chinese and Oriental folk medicine extensively utilized various fungi to effectively treat a wide range of human diseases.Avicenna, namely Abu Ali Hussin bin Abdallah ibn Hasan ibn Ali ibn Sino was born in 980 in the village of Afshana, the present-day Bukhara region in Uzbekistan; he described truffles as a cure for a variety of disorders, i.e., vomiting, wounds, and weakness in the "Al-Qanun fi't-Tibb" (The Canon of Medicine) [77] written in the 10th century as part of the Arabian Traditional Medicine.Avicenna recommended desert truffle juice used for common eye inflammations [78].The Canon of Medicine was used as a medical textbook in the Islamic world and Europe up to the 18th century.At the same time, Al-Biruni, namely Abu Raihon Muhammad ibn Ahmad Al Beruniy, born in Khorazm in Kiyat city in 973 in Uzbekistan; in his book "Kitab as Saidana fi-t-tibb" [79], morel (Morchella esculenta) is mentioned as a laundry detergent.When dried, the morels become white and inside, red.They are put in an antimony vessel andhave the color of dust with a black tint, and are a medicine for the eyes, applying such an ointment with a stick [78].However, despite these steps forward, comprehensive information on the edible and medicinal macrofungi is still unavailable in Uzbekistan in many aspects.In this article, we present current ethnomycological and medicinal uses, as well as other uses of medicinal macrofungi in Uzbekistan based on the literature review and our own data.
3.5.1.Auricularia mesenterica (Dicks.)Pers., Mycologia Europaea 1: 97 (1822) (Table 3; Figure 4A) Ethnomedicinal uses: In the past, A. mesenterica (Tripe Fungus) was used in Europe to treat throat ailments after being boiled in beer, milk, or vinegar, and it was often used to treat eye ailments [80].In Asian countries, A. mesenterica has been traditionally used as natural medicine; the fruiting bodies were used in China for cold and fever treatments, in addition to being employed to cure a variety of ailments, such as hemorrhoids, hemoptysis, and angina, as well as to strengthen the body [80].In Mexico, A. mesenterica was utilized in traditional medicine against anxiety and fear [81].Medicinal uses: Auricularia mesenterica, like other species of the genus Auricularia, has several pharmacological benefits including antioxidant and anti-inflammatory effects [82][83][84].Antioxidant properties were tested by Payamnoor et al. [85] and resulted in both methanol and ethanol extracts having high antioxidant properties; the results of this study suggested that A. mesenterica should be a good source for the designing of different drugs.Indeed, the production of antioxidant compounds, phenolic acids, flavonoids, and ascorbic acid, gives A. mesenterica antioxidant properties and these compounds act by inhibiting free radical chain formation and chelating transition metal ions, as well as scavenging reactive oxygen species [86].
Distribution and habitat: A. mesenterica was first described in Northern and Central Europe; it grows on different woody angiosperm plants [87]. A. mesenterica is also reported in North and South America [88,89] as well as Africa, Australia, Asia [90], and Uzbekistan [39].In Uzbekistan, A. mesenterica commonly grows on angiosperm wood plant species that are usually found throughout the year.
Medicinal uses: B. adusta commonly known as the smoky polypore or smoky bracket was investigated for various pharmacological properties as its antibacterial and antifungal properties.It is widely used in many countries in the treatment of inflammation in the human body and in diseases associated with various microorganisms, fungi, and viruses [93][94][95].It is important to mention that B. adusta is one of the most significant etiological fungi associated with chronic cough [96][97][98].
Distribution and habitat: It mostly occurs on various dead deciduous trees, rarely on coniferous trees.The species is widespread through temperate and boreal Eurasia, reported also from the tropics and temperate southern hemisphere [100][101][102].It is mostly found on Juglans, Populus, and Prunus species in Uzbekistan [33].
Edibility, aroma, and flavor: Inedible, smells intense and pleasant when fresh.
3.5.3.Cerioporus squamosus (Huds.)Quél., Enchiridion Fungorum in Europa Media et Praesertim in Gallia Vigentium: 167 (1886) (Table 3; Figure 4C) Ethnomedicinal uses: In TCM, this fungus was used as a pain reliever for the treatment of joint diseases [109].The list of diseases for which drugs based on the scaly polypore named Dryad's saddle are used is quite extensive: diseases of the bronchi and lungs (tuberculosis, pneumonia, bronchitis); dysfunction of the kidneys and bladder; oncology; gout; problems with the functioning of the pancreas; liver pathology; ulcer and gastritis; obesity.It is used to recover from injuries.
Distribution and habitat: It occurs in temperate and boreal zones of the northern hemisphere.Ryvarden and Gilbertson [214] reported 26 host genera including one conifer (Larix).It was found on the dried trunk of Acer tataricum subsp.semenovii, Juglans regia, Pistacia sp., Populus alba and Quercus sp. in Uzbekistan [33,102].
Edibility, aroma, and flavor: Fragrant, strong flavor, flour or mushroom odor, persisting long in later dried specimens; fruit body becomes hard and rough.It seems edible when young.
Medicinal uses: C. unicolor, commonly known as the mossy maze polypore, is still being studied scientifically in traditional medicine.According to modern scientific data, C. unicolor synthesizes secondary metabolites with a broad spectrum of biological activities [117,120].In addition to antidiabetic properties, antimicrobial, and hepatoprotective activities of exopolysaccharides of C. unicolor were also investigated in recent years [114][115][116].The results obtained so far have also shown antiproliferative [118,121], proapoptotic, and migration-inhibiting [121] properties of low molecular weight subfractions from the C. unicolor secretome, especially towards HT-29 colon cancer cells [119,121,122].
Distribution and habitat: Widely distributed in Asia, Europe, and North America from the Mediterranean to the boreal zone [215].It grows on dead or living, weakened broadleaved trees.It occurs on the trees of Celtis, Crataegus, Juglans, Populus, and Quercus in Uzbekistan [33].
3.5.5.Fomes fomentarius (L.) Fr., Summa vegetabilium Scandinaviae 2: 237 (1849) (Table 3; Figure 4E) Ethnomedicinal uses: As early as 400 BCE, Hippocrates referring to F. fomentarius used the Greek term "mykes"; this suggests that F. fomentarius commonly known as the tinder fungus is one of the earliest mushrooms to have been cited [216].Native Americans used the fungus as a diuretic and laxative, calming the nerves and against rheumatism [109,138].F. fomentarius was available in pharmacies in Europe until the 19th century under the name Fungus chirurgorum (wound sponge); it was applied by surgeons, dentists (during dental extractions), and barbers as a hemostatic wound dressing as well as warming compresses and cautery for moxibustion.The fungus extract was used to treat bladder issues, dys-menorrhea, and hemorrhoids to relieve pain [123,141,216,217].In traditional Chinese and Korean medicine, F. fomentarius was administered orally to treat gastrointestinal disorders, cirrhosis, oral ulcers, and inflammation.Furthermore, it is still being recommended as a supportive medicine for esophagus, gastric, and uterus carcinoma [127,138].
Local handicraft and other uses: The first evidence of using the fluffy, felted middle layer (amadou) of F. fomentarius dates back to about 5300 years ago.The species was identified in a first aid kit of the world-famous glacier mummy "Ötzi" (named after Ötztal, the valley on the borders between Austria and Italy where he was discovered); it was used as a tinder material and for spiritual purposes.Peintner and Pöder [218] pointed out the multiple uses of this fungus: fire production, styptic dressing, medicine, clothes, fishing articles, and decorative items as well as its symbolic and legendary role.The mentioned experiments on fire production and clothing fabrication demonstrate the efficiency of traditional techniques [123].F. fomentarius fruiting bodies were also used as snuff or for ritual smoking ceremonies in Eastern America, Austria, France, Germany, Japan, and Western Siberia [109,138,141,219].
Distribution and habitat: F. fomentarius grows on living and dead trees of hardwoods, and rarely on conifers [220].F. fomentarius has been reported on Juglans, Malus, Populus, Quercus, and Salix trees in Uzbekistan [33,102].
Edibility, aroma, and flavor: Inedible species with a fruity smell.It is acrid in taste.3) Ethnomedicinal uses: This species (previously named Piptoporus betulinus) is commonly known as the birch polypore; it is one of the most common species in central Europe and has been commonly used in folk medicine, especially in Baltic countries [140].It has also been used as tinder and anesthetic [140].In Russia, tea of the fungus was thought to have anti-fatiguing, soothing, and immunoenhancing properties.F. betulina was used in Bohemia for the treatment of rectal cancer and stomach diseases [141].Interestingly, antiseptic and pain reliever applications of the polypore were reported in Europe and the USA [138,141].F. betulina has been traditionally exploited as an antiparasitic, antimicrobial agent in the treatment of wounds for staunching bleeding [136].Infusion from F. betulina basidiome was popular, especially in Russia and Baltic countries, Hungary, and Romania for its nutritional and calming properties.Fungal tea was used against various cancer types, as an immunoenhancing, antiparasitic agent, and a remedy for gastrointestinal disorders [137,141,142].Antiseptic and anti-bleeding dressings made from fresh F. betulina fruiting body were applied to wounds and the powder obtained from dried ones was used as a painkiller [139,142,221].
Medicinal uses: Due to the broad spectrum of its phytochemical composition, this species has amazing diverse pharmacological properties such as anti-inflammatory, antiseptic, and antibacterial benefits.In addition, the astringent effect alone makes it good for making mushroom-containing herb tea or an immune tonic to be taken once a week to boost the immune system.F. betulina contains primary metabolites (as polysaccharides) and secondary metabolites (as triterpenes) that are beneficial for health [6,143,222].
Other uses: The velvety cut surface of the fruit body was traditionally used as a strop for finishing the edges of razors [223].Additionally, it was apparently also described for fine metal polishing, making ink blotters, and mounting insect collections.One use that would have been important in ancient times is that F. betulina takes a spark well and can be used to carry fire over long distances.Therefore, it allowed people to move around while maintaining easy access to fire [223].
Distribution and habitat: The geographic distribution of F. betulina is restricted to the northern hemisphere, including Europe, Asia, North Africa, and North America [99,224].This species was recorded on Betula tianschanica and unidentified birches (Betula sp.) from Uzbekistan [33].
Edibility, aroma, and flavor: Slightly bitter, strong and pleasant odor and an astringent bitter taste, inedible.3; Figure 4F) Ethnomedicinal uses: Fruiting bodies of G. adspersum have been studied at the Neolithic site of La Draga (Spain), confirming the ethnomycological importance of the species in Europe in prehistoric times.
Distribution and habitat: Occurs in the temperate region of Eurasia.The fungus has been recorded almost exclusively on hardwoods; it was found on a wide range of living deciduous trees, e.g., Alnus, Fagus, Fraxinus, Juglans, Morus, Pinus sp., Platanus, Robinia, Prunus, and Quercus, rarely on conifers trees [28,33,99].
Edibility, aroma, and flavor: Inedible, not significant odor but bitter taste.
3.5.8.Ganoderma applanatum (Pers.)Pat., Bulletin de la Société Mycologique de France 5: 67 (1889) (Table 3) Ethnomedicinal uses: It has been used in traditional medicine for many years in the form of a tea or an aqueous extract, which generally imparts a sensation of intense heat when consumed.The taste and smell of this fungus may vary depending on the host plant [225].In TCM, G. applanatum is used for digestive disorders to reduce excess phlegm, pain, and fever, lowering blood glucose and improving immunity.Additionally, it is used as an antiviral and antitumor agent [225].Additionally, the health problems associated with potency, hypertension, asthma, bronchitis, prostatitis, mental disorders, and cardiovascular and circulatory systems were also largely solved.Since ancient times, G. applanatum has been used in eastern Asia for its medicinal benefits in treating conditions like cancer, hepatitis, diabetes, erectile dysfunction, hypertension, asthma, bronchitis, prostate, and mental disorders as well as for the proper operation of the cardiovascular and circulatory systems (https://progrib.ru/trutoviki/trutovik-ploskiy.html,accessed on 3 June 2023).In Nigeria, G. applanatum has been used as an antioxidant, hypoglycemic, and antihypertension agent [226].It has been used to treat internal growth, heart problems, and cancer in Cameroon [145].The powder of dried G. applanatum fruiting body is added to vegetables in very small quantities during cooking with the belief that it reduces the chances of disease in India [227].It has also been used in Serbia for strengthening the immune system as tea [18].
Medicinal uses: G. applanatum (Artist's conk) produces various bioactive compounds that exhibit antiallergic, anticancer, antifibrotic, antihyperglycemic, antimicrobial, antioxidant, antitumor, hepatoprotective, hypoglycemic, immunomodulatory, liver protective properties as well as inhibition of aldose reductase enzyme, Epstein-Barr and influenza virus [9,11,124,146,148,154].Currently, G. applanatum and other species of the genus Ganoderma are used in China and Japan for the treatment and prevention of hepatitis, hypertension, chronic bronchitis, bronchial asthma, hyperglycemia, rheumatism, connective tissue, and esophageal cancer (a dangerous tumor with epithelial cells), arthritis, tuberculosis, and many other diseases [144,147,228,229].
Distribution and habitat: Occurs in the temperate zone of Eurasia mainly on broadleaved trees, and more rarely on conifer trees [33,230].
Edibility, aroma, and flavor: Inedible, no significant odor and bitter taste.
3.5.9.Grifola frondosa (Dicks.)Gray, a Natural Arrangement of British Plants 1: 643 (Table 3) Ethnomedicinal uses: G. frondosa also known as hen-of-the-woods and maitake was a highly valued commodity in feudal Japan, with local lords exchanging it for an equivalent weight in silver.Thus, the Japanese name "dancing mushroom" comes from commoners in Japan who would dance for joy when they discovered G. frondosa, knowing they would be richly rewarded for their discovery.The mushroom was so highly valued in Japan, and the expert mushroom foragers would keep their harvest areas so secret that they would only reveal their locations after their death in their wills [163].Based on literature data, in mainland Japan, China, and some other Asian countries, G. frondosa was popularly consumed for centuries as traditional medicine as curative herbal medication or health foods based on its enticing taste [18,164,231,232].
Distribution and habitat: This fungus is distributed in the temperate zone of the Northern Hemisphere, temperate forests in eastern North America, Europe, and Asia [163,238].The most common substrate is Quercus spp.but may also occur on other hardwoods and occasionally on conifers [33,102,215].
Edibility, aroma, and flavor: Edible of choice when young.Tough and requiring long cooking when ripe.Pleasant, fragrant, nutty flavor, persistent and succulent, but slightly acidic, more pronounced when ripe.
3.5.10.Inonotus hispidus (Bull.)P. Karst., Meddelanden af Societas pro Fauna et Flora Fennica 5: 39 (1879) (Table 3; Figure 5A) Ethnomedicinal uses: Inonotus hispidus commonly known as the shaggy bracket is used in traditional medicine to treat dyspepsia, cancer, and diabetes.It is also used to treat digestive disorders (parasites, diarrhea, general internal cleaning) as well as diseases of the heart, liver, stomach, and abdominal pain.Furthermore, when used for cleansing the genitals during menstruation and for newborns, the infusion of I. hispidus exhibits antiseptic properties [171].I. hispidus is reported to be used as ancient medicinal materials and health care products in Chinese traditional medicine.It is used as a diuretic, an astringent, and to treat canker sores and inflammation in folk culture [167].
Medicinal uses: Numerous studies have confirmed the anti-inflammatory, antifungal, antimicrobial, antioxidant, and antiviral properties, as well as antitumor, immunomodulatory, immunostimulatory, antiproliferative, and cytotoxic effects of I. hispidus extracts [168].Studies have shown promising immunomodulatory activity of extracts from the I. hispidus basidiomes, with presence of the bioactive substances such as hispolon and hispidin [170]: natural killer T cell activity and function increased with a dose of mushroom fruiting body extract.Later, it is concluded that I. hispidus may be a new source of neurotrophic and protective agents against neurodegenerative diseases [172].Neurodegenerative diseases, which mainly affect the elderly, include Alzheimer's, Parkinson's, Huntington's, and Pick's disease, and are characterized by the slow death of specific groups of nerve cells and increasing atrophy of the corresponding parts of the brain or spinal cord [169].In a recent study, Zhang et al. [173] discovered that I. hispidus demonstrates hypolipidemic effects in laboratory mice by effectively suppressing inflammation caused by oxidative stress.
Distribution and habitat: Grows on living Quercus spp.but also on a very large number of angiosperms woody plants.Common and widespread species that can be found in gardens, parks, orchards, and forests.Europe and Asia have a temperate distribution [33,99,220].
Edibility, aroma, and flavor: Inedible, aroma and flavor are irrelevant.3; Figure 5B) Ethnomedicinal uses: Its common names are crab-of-the-woods, sulfur polypore, sulfur shelf, and chicken-of-the-woods.According to Icones of Medical Fungi from China [114], L. sulphureus fruit bodies are thought to be capable of regulating the human body, improving health, and defending the body against illnesses; it has also been used to repel mosquitoes and midges by burning fruit bodies [114].L. sulphureus has long been used in Asian herbal medicine [239].In folk medicine, it is used in powders, water, and alcohol tinctures.It is widely used in the treatment of diseases such as pyretic diseases, coughs, gastric cancer, and rheumatism [17].
Medicinal uses: This fungus is known as a source of antimicrobial, antitumor, anti-inflammatory, anticoagulant, antioxidant, cytostatic, hypoglycemic, and immunostimulant agents as well as a producer of HIV-1 reverse transcriptase inhibitors [174,239].Due to their long history of medicinal uses, the biologically active compounds and extracts from L. sulphureus exhibit a broad spectrum of pharmacological activities as anti-diabetic, anti-malarial, anti-thrombin, anti-ulcer, antiviral, hepatoprotective, and immunomodulating [17,175,240].
Distribution and habitat: L. sulphureus has been treated as a cosmopolitan species present on all continents except Antarctica, from boreal to subtropical and tropical zones [241].The most common hosts of L. sulphureus in Europe are hardwoods such as Quercus, Fagus, Populus, Prunus, Pyrus, Robinia, and Salix, and rarely also conifers such as Cupressus or Taxus [33,99,220].L. sulphureus was recorded on Acacia, Acer, Juglans, Prunus, Robinia, and Quercus in Uzbekistan [33].
Edibility, aroma, and flavor: Edible when young, strong odor and pleasant taste.
3.5.12.Lentinus tigrinus (Bull.)Fr., Systema Orbis Vegetabilis 1: 78 (1825) (Table 3; Figure 5C) Ethnomedicinal uses: L. tigrinus (Tiger Sawgill) is widely used as food, medicine, brain tonic, and against anger.Powder taken in hot water relieves dry cough and asthma; the raw materials are also useful for wet cough.Mixing raw fruit bodies with lemon juice improves gastrointestinal function and enhances digestion [177].Indigenous communities considered this mushroom as a potential source of antibacterial drugs and as remedies for arthritis, cough and colds, fever, headache, hypertension, skin diseases, stomachaches, and toothaches.They usually boil or grind the mushroom to obtain an extract; they drink the broth or apply the fungus directly to infected body parts [183].
Medicinal and environmental uses: This mushroom has antimicrobial and antioxidant [179], hypoglycemic and antidiabetic [176], anti-inflammatory [178], anticancer [181,182], and immunomodulatory properties [180].In addition, Mohammadnejad et al. [181] reported the anticancer potentiality of a soluble protein fraction of L. tigrinus that showed greater antiproliferative and cytotoxic activities against PC3 cells; this suggests that the soluble protein fraction of this mushroom may be considered a potent anticancer compound.Recently, Pourianfar et al. [182] found that wild Iranian L. tigrinus strain may be regarded as a source of macro-and micronutrients with specific anticancer potential against MCF-7 cells; however, it poses little risk to humans when consumed in small amounts.
Distribution and habitat: Distributed in the Northern Hemisphere, Eurasia, and North America.In Central Asia, it is common in forests, gardens, and along roadsides, especially where walnuts, poplars, and willows grow [33,102].This fungus occurs on hardwood, usually in floodplain regularly moistened forests.It grows mainly on Salix and Populus, less common on other hardwoods.Edibility, aroma, and flavor: Edible, fruity, pleasant odor, and pleasant mushroom but later astringent flavor.
3.5.13.Phellinus igniarius (L.) Quél., Enchiridion Fungorum in Europa Media et Praesertim in Gallia Vigentium: 172 (1886) (Table 3) Ethnomedicinal uses: The fruiting bodies of P. igniarius (Willow Bracket) have been historically used as a folk medicine for the treatment of endometrorrhagia in gynecology [188].This species has the ability to scavenge free radicals and prevent other diseases in traditional medicine; it would reduce the risk of heart attack, stroke, and cancer.Drinking its tinctures and broths is used as a treatment for various injuries, and inflammations [109].P. igniarius was burned, mixed with tobacco, and chewed.The alkaline properties of ash are known to increase the penetration of nicotine into the bloodstream.The Arctic tribes boiled the pulp of the fruitbodies and drank it as a broth to relieve stomach ailments or pain.A tincture or tobacco prepared with this fungus has also been used to protect the liver in patients with liver damage due to alcohol abuse.P. igniarius tea is a very popular antioxidant, which is believed to inhibit cancer [184].
Medicinal uses: This fungus is rich in biologically active substances with therapeutic potential.Mycochemical studies have proved the presence of polysaccharides, phenolic compounds, and terpenoids.These compounds showed biological activities such as antiangiogenic, anticancer, antioxidant, and antiviral.Research studies conducted using modern analytical methods have advanced the knowledge of the potential therapeutic uses of compounds isolated not only from the fruiting bodies but also from biomass obtained with in vitro biotechnological methods [148,[187][188][189].
Distribution and habitat: The fungus found on hardwood living and later dead trees.P. igniarius in the narrow sense appears to occur in Uzbekistan and other Central Asian countries; it is recorded on Acer, Juglans, Lonicera, and Salix [33].
Edibility, aroma and flavor: Inedible  3; Figure 5D) Ethnomedicinal uses: Recently, Zhou et al. [191] defined "Sanghuang" as one of the most important groups of medicinal mushrooms with fourteen described species in the genus Sanghuangporus including S. lonicerinus, which have been used in TCM for the past two centuries.Wu et al. [190] reported S. lonicerinus is commonly used in Hubei and other places, as well as by the Tujia ethnic minority.All species in Sanghuangporus can be considered to be medicinal macrofungi [190,191].
Medicinal uses: Based on modern pharmacological studies, S. lonicerinus was reported to have multifaceted biological activities, including antiproliferative [186], and hepatoprotective effects [185].Shen et al. [243] recently reported widespread misidentifications of traditional medicinal mushrooms in the Sanghuangporus genus via ITS barcoding and designation of reference sequences.
Distribution and habitat: S. lonicerinus is distributed throughout the republics of Central Asia, Iran, and Russia and is reported on Lonicera spp.[33,102].
3.5.15.Schizophyllum commune Fr., Observationes mycologicae 1: 103 (1815) (Table 3; Figure 5E) Ethnomedicinal uses: S. commune (Common split gill) has been renowned in Far Eastern countries as medicine for hundreds of years [192].In relation to this, it has been used in TCM in the form of infusions to treat leucorrhea and to regulate blood pressure [245].Milenge Kamalebo et al. [246] claimed that people used S. commune in the treatment of breast inflammation and wounds.
Medicinal uses: S. commune is a potent and well-studied edible medicinal mushroom with analgesic [195], antidiabetic [197,247], anti-inflammatory [194], antimicrobial [198,199,248], antioxidant [193,196,197,199], antitumor, chemoprotective, immunostimulating [240], and neuroprotective [199] effects.In this regard, Umeo et al. [249] mentioned that S. commune is an important producer of hydrolytic enzymes with biotechnological potential, antioxidant, and immunomodulatory activities and is also used in the form of antitumor polysaccharide schizophyllan (SPG) as an adjuvant treatment for tumors.Garcia et al. [250] reviewed that the most promising effects of SPG seem to be antitumor and immunomodulation actions, based on its clinical use for the treatment of several cancers in Japan.Vu et al. [201] recently demonstrated that S. commune β-glucan improved intestinal health by enhancing water absorption, proliferating epithelial cells, and increasing lubricating mucin production, which is essential for the treatment of constipation.Moreover, the administration of β-glucan prevents the risk of atherosclerosis, diabetes, intestinal inflammation diseases, and obesity based on the reduction in serum glucose levels and biomarkers associated with liver injury and up-regulating of HDL [201].In addition, Zeynali et al. [202] reported the antibacterial activity and healing properties of a dressing made from an S. commune-derived chitin glucan complex using an animal model of second-degree burn.These nanofibers exhibited biocompatibility, as they do not cause toxicity to fibroblast cells and, in fact, enhance their proliferation and adhesion capabilities and potentially could be utilized as wound dressings.
Distribution and habitat: This species is a cosmopolitan species that colonize living trees and dead wood of broadleaved trees, rarely conifers.In Uzbekistan, this fun-gus is found on Celtis caucasica, Juglans regia, Malus domestica, Morus alba, Populus sp., Prunus armeniaca, and Salix pentandra Ehrh.[33].
Edibility, aroma, and flavor: Not distinctive.But in youth, the common slit-leaf has tender flesh and excellent taste.In European and American countries, it is considered inedible, largely due to cultural tastes, small stature, and its presumed leathery texture.Literature reports on S. commune have mainly implicated the fungus with multiple pulmonary manifestations ranging from sinusitis, allergic bronchopulmonary mycoses, asthma, chronic eosinophilic pneumonia, and so on [251,252].
3.5.16.Stereum hirsutum (Willd.)Pers., Observationes Mycologicae 2: 90 (1800) (Table 3; Figure 5F) Ethnomedicinal uses: Stereum hirsutum called hairy curtain crust fungus has been used as a food and folk medicine in Chinese society [204,205].It has been used in traditional medicine to treat diabetes and dyspepsia.It is also applied in India as an antiseptic [203].Mišković et al. [207] mentioned that the fruiting bodies of S. hirsutum were used as traditional folk medicine in China and Korea for cancer treatment.
Medicinal uses: Duan et al. [204] summarized that the fermented mycelia of S. hirsutum produced biologically active secondary metabolites, such as antibacterial epidioxysterols, benzoate derivatives, bioactive vibralactone, multiple active sesquiterpenoids, and phytotoxic active acetylenic compounds among others.In summary, most of them showed a broad diversity of biological activities including antibacterial, antifungal, anti-nematocidal, antiviral, autophagy-inducing activity, pancreatic lipase inhibition, and antiparasitic in malaria [206], as well as antidiabetic, antimicrobial, antioxidant, cytotoxic, and neuroprotective activities, among others [200,207,208].
Distribution and habitat: Widespread in the northern and southern hemispheres [253], usually in the temperate zone.This species grows on dead trunks and branches, and also on dead branches and exposed dead wood of living trees.In Uzbekistan, Stereum hirsutum was recorded on eight tree genera: Acer, Celtis, Crataegus, Fraxinus, Juglans, Populus, Quercus, and Salix [33].
Edibility, aroma, and flavor: Inedible, no special smell or taste.
3.5.17.Trametes versicolor (L.) Lloyd, Mycol.Writ.6 (65): 1045 (1920) (Table 3) Ethnomedicinal uses: Trametes versicolor, the Turkey tail fungus (formerly known as the Many-Zoned Polypore), is one of the best well-known traditional medicinal mushrooms used in China for over 2000 years [211].In TCM, this fungus has been used as a tea or soup to treat cancers of the liver, jaundice, fever, spleen as well as heart, stomach, and sometimes kidney disorders [254].It was also used as a tonic for the regulation of asthma, coughs, immune functions, and in the case of increased appetite [254].T. versicolor was frequently used in TCM for its immunomodulatory and antitumor activities [255].It was considered useful in dispelling fever, improving immune functions, eliminating toxins, and strengthening the physical health of the patient [209].On the other hand, in the clinical practices of TCM, T. versicolor was often indicated for various types of cancer, chronic hepatitis, and infections of the upper respiratory, urinary, and digestive tracts [209,256].
Medicinal uses: T. versicolor, an important non-edible medicinal species, has gained widespread popularity due to its broad spectrum of beneficial properties including antiacetylcholinesterase, antidiabetic, anticoagulant, anti-inflammatory, antimicrobial, antiobesity, antioxidant, antitumor, cardioprotective, hepatoprotective, hypocholesterolemic and hypolipidemic activities as well as immunoregulatory and immunostimulatory effects [210,212,213].In addition, it is the most commonly used fungus for various cancers affecting the breast, cervix, lungs, esophagus, and uterus, as well as when leukemia, lymphoma (lymphatic tissue that causes swollen nodes), melanoma (skin cancer), and brain cancer.Recently, He et al. [188] isolated musarin, a novel protein from T. versicolor that has shown potency to be developed as a promising new therapeutic drug candidate to support colorectal cancer treatment.
Distribution and habitat: The species has a global distribution and is widespread in temperate Eurasia [214].This species is commonly found on weakened or wounded living trees, dead trees, dead large branches, and stumps.In Central Asia, it occurs on Betula, Crataegus, Celtis, Juglans, Lonicera, Malus, Prunus, and Quercus trees [33].

Conclusions
Firstly, the present investigation focuses on the diversity of macrofungi collected from several provinces of Uzbekistan in 2021-2022 using morphological characters and molecular data.Seventeen macrofungal species of basidiomycetous from 11 families and 16 genera have been identified, possessing edible and/or medicinal properties.Ethnomedicinal and medicinal applications, morphological characteristics, molecular phylogeny, host preferences, geographical distribution, and habitat of the collected macrofungi have been provided.
Secondly, our study is the first to focus on the ethnomycological diversity and ethnomedicinal uses of medicinal fungi from Uzbekistan, knowing that basidiomycete macrofungi are ecologically and economically pathogenic, nutritional, and medicinal resources.In the provinces of Uzbekistan, 17 species of basidiomycetous macrofungi of great ethnomycological importance from ten families and sixteen genera have been identified, possessing edible and/or medicinal properties.
A comprehensive overview of ethnomycological knowledge, traditional and modern medicinal uses, and pharmacological data of macrofungal species has been developed.This information serves as valuable scientific information on the use of medicinal mushrooms in folk and modern medicine across the country.These reports constitute effective scientific data on the various uses of medicinal mushrooms in folk and modern medicine in Uzbekistan, with a broad spectrum of applications.
Finally, the information described in this work can be used to better utilize the medicinal value of medicinal mushrooms growing in Uzbekistan.In addition to highlighting their use in medicine, it should also be recognized that the creation of a national database of macrofungi in Uzbekistan is becoming a topical issue today.Moreover, it is to be recommended that they be studied phytochemically with the latest pharmaceutical procedures that can eventually lead to drug discovery.

Figure 2 .
Figure 2. Phylogenetic position of newly sequenced Uzbekistan specimens of medicinal macrofungi inferred from the ITS region.The topology is generated by the maximum likelihood algorithm.The maximum likelihood bootstrap values and the Bayesian posterior probabilities, when simultaneously above 50% and 0.8, respectively, are shown at the nodes.Coniophora arida and Gomphidius roseus from Boletales were selected as outgroup taxa.

Figure 3 .
Figure 3. Distribution of medicinal macrofungi in provinces of Uzbekistan.The names of the mushroom distribution areas are abbreviated as follows: All region-AR; Jizzakh province-JP; Tashkent province-TP; Namangan province-NP; Fergana province-FP; Andijan province-AP; Samarkand province-SP; Tashkent Botanical Garden-TB.

3. 5 .
Taxonomic Treatment, Distribution, Ecology of Medicinal Macrofungi, and Their Uses in Ethnomedicine, Modern Medicine, and Other Fields

Table 2 .
Voucher numbers and corresponding GenBank accession numbers of sequences used for phylogenetic analyses.

Table 3 .
Ethnomycology and ethnomedicinal uses of the wood-inhabiting basidiomycete macrofungi from Uzbekistan (species name, local name, folk and modern medicinal uses, host plants).