DdaCrz1, a C2H2-Type Transcription Factor, Regulates Growth, Conidiation, and Stress Resistance in the Nematode-Trapping Fungus Drechslerella dactyloides

The Ca2+/calmodulin-dependent signaling pathway regulates diverse cellular processes. Calcineurin is a calcium-dependent phosphatase acting in fungi mainly through Crz1, a zinc finger transcription factor. Although the likely involvement of Ca2+ in fungal carnivorism has been documented, how Crz1 functions in nematode-trapping fungi remains unknown. Here, we identified the Crz1 gene (named as DdaCrz1) in Drechslerella dactyloides, a species that forms constricting rings to trap nematodes. The deletion of DdaCrz1 significantly reduced hyphal growth and conidiation, trap formation, and ring cell inflation. Moreover, the mutation increased sensitivity to Mn2+ but decreased sensitivity to Ca2+, Mg2+, Zn2+, and Li+. Similarly, the mutant showed increased tolerance to osmotic stress but was more sensitive to Congo red, a cell wall-damaging agent. Our results confirmed the critical roles of the Ca2+/calmodulin-dependent signaling pathway in regulating growth, conidiation, and the stress response, and suggested its involvement in trapping nematodes.


Introduction
Calcineurin, the Ca 2+ /calmodulin-dependent serine-threonine-specific protein phosphatase that is highly conserved among single-cell and multicellular eukaryotes, is involved in regulating diverse processes [1][2][3][4][5]. The Ca 2+ -dependent signaling pathway is triggered by receptors that perceive external stimuli, resulting in Ca 2+ binding to calmodulin, which subsequently activates calcineurin [6,7]. Calcineurin is composed of a catalytic subunit A (CNA) and a regulatory subunit B (CNB) [8,9]. The expression of the genes downstream of calcineurin is mainly activated by the transcription factor calcineurin-responsive zinc finger 1 (Crz1). As the primary mediator of calcineurin signal transduction, Crz1 is dephosphorylated after calcineurin activation and transferred from the cytoplasm to the nucleus, where it binds to calcineurin-dependent response elements (CDREs) in the promoters of target genes [10,11]. Crz1 is involved in regulating a variety of cellular processes, including cell wall integrity, ion transport, redox processes, carbohydrate metabolism, protein phosphorylation, and vesicle-mediated transport. The Crz1 in yeasts and filamentous fungi has been extensively characterized. In Saccharomyces cerevisiae, Crz1 regulates the expression of FKS2, PMC1, PMR1, ENA1, GPX2, and RCN1, which are involved in maintaining the cell wall integrity, ion transport, and glucose metabolism [10,12]. In Candida albicans, the deletion of Crz1 caused hypersensitivity to alkaline cations, detergent SDS, and fungicide azoles [13][14][15], and impacted cation homeostasis and virulence [16]. In Botrytis cinerea, a filamentous fungus that infects diverse plants, the Crz1 gene is involved in hyphal morphology, conidiation, sclerotia formation, and host penetration [17]. The knockout of CrzA in Aspergillus fumigatus resulted in decreased conidiation and virulence and increased sensitivity to ion stress [18]. The deletion of Crz1 in Magnaporthe oryzae impaired its growth in the presence of Ca 2+ or cell wall degradation agents and reduced conidiation and virulence [19]. These studies underscored the significance of Crz1 for growth, conidiation, virulence, and stress defense in fungi.
Nematode-trapping fungi (NTF), which consume nematodes as nutrient sources, influence nematode dynamics in nature. The hyphae of NTF can develop diverse trapping devices, including adhesive networks, adhesive knobs, adhesive columns, and constricting rings [20,21], which can capture vermiform nematodes as nutrient resources [22,23]. Calcium ion is a ubiquitous second messenger in cells. The Ca 2+ /calmodulin-dependent protein kinases (CaMKs) [24] and the low-affinity calcium channel protein [25] encoded by Arthrobotrys oligospora, an NTF, have been shown to be involved in hyphae growth, conidiation, trap formation, and stress management. Chen et al. [26] proposed that nematodes activate the G-proteins in the ring cells of Drechslerella dactyloides, a model for constricting ring-forming NTF. This activation increases cytoplasmic Ca 2+ , causing the activation of calmodulin and the subsequent opening of water channels for the inflation of constricting rings. These studies indicated that the Ca 2+ signaling pathway plays crucial roles in NTF.
Our preliminary investigation showed that FK506, a calcineurin inhibitor, could significantly inhibit the growth and trap formation of D. dactyloides, suggesting the involvement of Crz1 in the growth and predatory process. Although a CaMK gene of D. dactyloides was cloned and characterized in 2002 [27], its role in Ca 2+ /calmodulin-mediated signaling remains unknown. We identified DdaCrz1, a Crz1 homolog, in D. dactyloides and investigated how it affects the growth, conidiation, trap formation, and stress resistance via gene deletion.

Sequence and Phylogenetic Analysis of DdaCrz1
The Crz1 transcription factor DdaCrz1 encoded by D. dactyloides was identified via BLASTP using the amino acid sequences of Crz1 encoded by Aspergillus fumigatus, Botrytis cinerea, and Schizosaccharomyces pombe as queries for searching in the NCBI database. The isoelectric points (pI) and molecular weights were predicted using the online software ExPASy-ProtParam tool (https://web.expasy.org/protparam/, accessed on 1 February 2022), and conserved functional domains were identified using SMART (http://smart. embl-heidelberg.de/, accessed on 1 December 2021) and InterProScan (http://www.ebi.ac. uk/interpro/, accessed on 1 December 2021)). Additional Crz1 orthologs encoded by other fungi were retrieved from the NCBI database using BLASTP. A phylogenetic tree based on amino acid sequences of the Crz1 proteins encoded by diverse fungi was constructed using the MEGA7 software package.

Gene Knockout of DdaCrz1
The DdaCrz1 gene was deleted using a homologous recombination-mediated strategy. The 5 (2992 bp) and 3 (3011 bp) ( Figure S1) flanking regions of the DdaCrz1 open reading frame (ORF) were cloned into the upstream and downstream of the hygromycin B resistance gene cassette on the pAg1-H3 vector using the ClonExpress ® II One Step Cloning Kit C112 (Vazyme, Nanjing, China) [30,31]. The 5 flanking region was amplified using primers Crz1-5F and Crz1-5R, and the 3 flanking region was amplified using primers Crz1-3F and Crz1-3R (Table S1). Subsequently, the resulting plasmid was transformed into Agrobacterium tumefaciens strain AGL-1 (Biomed, Beijing, China). DdaCrz1 was deleted via an improved A. tumefaciens-mediated transformation (ATMT) method [31,32]. The AGL-1 transformant containing the plasmid was co-cultured with D. dactyloides conidia (ca. 10 6 ) harvested from 14-day-old culture on CMA for 7 days. Subsequently, the co-cultures were covered with PDA containing 100 µg/mL hygromycin B (Leagene, Beijing, China) and 400 µg/mL cefotaxime sodium (Solarbio, Beijing, China) for selecting transformants. The resulting transformants were maintained on PDA plates containing 100 µg/mL hygromycin B. Genomic DNA was extracted from the transformants using cetyltrimethylammonium bromide (CTAB) as previously described [33]. Individual transformants were analyzed for the existence of the hygromycin B resistance gene and the deletion of the DdaCrz1 gene using PCR [34] with two pairs of primers, HYG-540F and HYG-540R, and Crz1-667F and Crz1-667R (Table S1). Confirmed mutants were analyzed via quantitative real-time PCR (qPCR) to check the lack of transcripts. Cultures derived from single conidia were transferred onto PDA plates containing 100 µg/mL hygromycin B for 5 generations [34] to ensure the stability of the mutants before phenotypic characterization.

Complementation of the DdaCrz1 Mutant
To complement the mutation created by DdaCrz1 deletion, a copy of DdaCrz1 was introduced into ∆DdaCrz1-27 via ATMT [32]. The complementation plasmid was constructed using the ClonExpress ® II One Step Cloning Kit C112 (Vazyme, Nanjing, China). The DdaCrz1 gene, including the 5 (2079 bp) and 3 (2032 bp) flanking regions, was amplified using primers Re-Crz1-F and Re-Crz1-R (Table S1). Subsequently, the amplified fragment was cloned into a modified pAg1-H3 vector [31] with the G418 resistance gene using one-step cloning. After ATMT, transformants were selected on PDA containing 200 µg/mL G418 (Leagene, Beijing, China). The resulting transformants were screened by PCR using two pairs of primers, G418-617F/G418-617R and Re-Crz1-F/Re-Crz1-R. Subsequently, the transformants containing the introduced copy of DdaCrz1 were transferred onto PDA plates supplemented with 200 µg/mL G418 for at least 5 generations [34].

Comparison of Mycelial Growth and Conidial Production
The growth rates of strain 29 and its mutants under different nutritional conditions were compared by inoculating 5 mm-diameter discs punched from the edges of 14-day-old CMA culture onto PDA, TG, and CMA plates. During 12 days of incubation at 28 • C, their colony diameters were measured every two days. Conidia from the 14-day-old CMA cultures were harvested by water containing 0.1% Tween-20. The number of conidia produced by each strain was counted using a hemocytometer.

Analyses of Mycelial Growth under Different Stresses
Discs (5 mm in diameter) punched from the edges of 14-day-old cultures of strain 29 and its mutants on CMA plates were placed on the center of individual plates supplemented with various agents stressing fungi. In the initial experiment, the following agents were tested: NaCl and KCl at 0. Relative growth inhibition (RGI) caused by each chemical stress was calculated using the equation (Dc − Dt)/(Dc − d) × 100%, where Dc and Dt refer to the diameters of the unstressed (control) and stressed colonies, respectively, and d is the diameter of the inoculated discs [35,36].

Constricting Ring Formation and Inflation after Introducing Nematodes
To compare the ability to form traps, ca. 1000 conidia harvested from strain 29 and its mutants were inoculated onto 2% WA plates and incubated at 28 • C for 3 days. Each plate was supplemented with approximately 1000 C. elegans. At 16 h and 24 h after the induction, the numbers of constricting rings and inflated constricting rings within a 4 cm 2 area of each colony were counted.

RNA Extraction
Conidia of strain 29 and its mutants were plated onto WA plates (about 10 5 per plate) overlaid with a cellophane membrane [37]. Mycelia above the membrane were harvested after 3 days of incubation, firstly before adding nematodes (0 h) and then 8 h, 16 h, and 24 h after adding approximately 1000 nematodes per plate. RNA was extracted using TRIzol (InvitrogenTM, Carlsbad, CA, USA) as previously described [38].

Real-Time PCR (RT-PCR) Analysis
Levels of transcripts from DdaCrz1 were measured using qPCR. RNAs extracted from strain 29 and its mutants were reversely transcribed into cDNA using a FastKing RT Kit with gDNase (Vazyme, Nanjing, China). The qPCR reactions were performed using SYBR Green Real-Time PCR Master Mix (Vazyme, Nanjing, China), and the β-tubulin gene was used as an internal standard. To calculate the relative transcriptional level of DdaCrz1, the 2 −∆∆Ct method was used [39], and these experiments were performed in triplicate.

Statistical Analysis
Experimental data were presented as mean ± SD. The p-values < 0.05 using Student's t-test were considered statistically significant [36]. All statistical analyses were conducted using GraphPad Prism version 5.00 (GraphPad Software, San Diego, CA, USA).

Identification of the Crz1 Gene of D. dactyloides
The gene (DdaCrz1) encoding the calcineurin-responsive Crz1 protein of D. dactyloides (CGMCC3.20198) produces a protein of 663 amino acids with a predicted molecular weight and pI of 73.22 kDa and 6.25, respectively. Two well-conserved zinc finger motifs (residues of 440-462 and 468-490) are present at the C-terminus ( Figure 1A). Additionally, we identified two putative calcineurin-docking domains ( Figure 1A). The sequence of DdaCrz1 is highly similar (77.73-91.47% identity with 100% coverage) to its orthologs encoded by other nematode-trapping fungi ( Figure 1B), with the highest identity to those encoded by the constricting ring-forming fungi D. brochopaga (91.47%) and D. stenobrocha (82.28%). The sequence similarity to orthologs encoded by non-NTF in Ascomycetes such as Aspergillus nidulans (46.7% identity; 78% coverage) and yeast (significant similarity limited to the zinc finger motifs) was much lower.

DdaCrz1 Disruption and Complementation
A homologous recombination-mediated strategy was applied to delete DdaCrz1 ( ure 2A and S1). Among 96 transformants obtained using ATMT, two deletion muta (ΔDdaCrz1-8 and ΔDdaCrz1-27) were selected ( Figure S2) for subsequent analysis. G disruption was confirmed by PCR using genomic DNA extracted from these mutants ( ure 2A). A qRT-PCR analysis showed that DdaCrz1 expression in strain 29 increased 1.5-fold after introducing C. elegans for 16 h( Figure 2C), suggesting the involvemen DdaCrz1 in the predatory process. Deletion of DdaCrz1 was confirmed by the lack of g expression ( Figure 2D). The mutation was complemented by introducing a copy of gene into ΔDdaCrz1-27 via ATMT ( Figure 2B), and one transformant (ΔDdaCrz1-C) selected from 48 transformants ( Figure S3). The expression level of DdaCrz1 betw strain 29 and the complemented mutant was similar ( Figure 2D).

DdaCrz1 Disruption and Complementation
A homologous recombination-mediated strategy was applied to delete DdaCrz1 (Figures 2A and S1). Among 96 transformants obtained using ATMT, two deletion mutants (∆DdaCrz1-8 and ∆DdaCrz1-27) were selected ( Figure S2) for subsequent analysis. Gene disruption was confirmed by PCR using genomic DNA extracted from these mutants (Figure 2A). A qRT-PCR analysis showed that DdaCrz1 expression in strain 29 increased to 1.5-fold after introducing C. elegans for 16 h (Figure 2C), suggesting the involvement of DdaCrz1 in the predatory process. Deletion of DdaCrz1 was confirmed by the lack of gene expression ( Figure 2D). The mutation was complemented by introducing a copy of the gene into ∆DdaCrz1-27 via ATMT ( Figure 2B), and one transformant (∆DdaCrz1-C) was selected from 48 transformants ( Figure S3). The expression level of DdaCrz1 between strain 29 and the complemented mutant was similar ( Figure 2D).

Deletion of DdaCrz1 Decreased Vegetative Growth and Conidiation
Colony growth rates on CMA, PDA, and TG agar were similar between strain 29 and ∆DdaCrz1-C, but that of ∆DdaCrz1 was significantly decreased. The colony diameters of strain 29 and ∆DdaCrz1-C were 46.7 ± 1.5 mm and 46.1 ± 0.8 mm, respectively, while two ∆DdaCrz1 mutants were 33.2 ± 2.7 mm and 34.0 ± 2.3 mm in colony diameter after 12 days of incubation on CMA ( Figure 3B and Table S2). The growth rates of the two ∆DdaCrz1 mutants were 53.7% and 56.2% of that of strain 29 on PDA ( Figure 3C and Table S3) and 56% and 58% on TG agar ( Figure 3D and Table S4). The ∆DdaCrz1 mutants formed sparse aerial hyphae compared with strain 29 and ∆DdaCrz1-C on all three media ( Figure 3A). The number of conidia produced by the ∆DdaCrz1 mutants was reduced (42% of that produced by strain 29 and ∆DdaCrz1-C) after 14 days of incubation on CMA supplemented with 2 g/L KH 2 PO 4 ( Figure 3E and Table S5).

Disruption of DdaCrz1 Decreased Cell Wall Integrity
Cell wall permeability can be affected by Congo red (CR), a cell wall inhibitor that specifically binds to β-1,3-glucose, and SDS, a surfactant that removes lipids associated with the cell wall. CR and SDS were applied to test the involvement of DdaCrz1 in maintaining cell wall integrity (CWI). The two ΔDdaCrz1 mutants were more sensitive to CR ( Figure 4A,B and Table S6), but not to SDS ( Figure 4A,C and Table S6), in comparison with strain 29 and ΔDdaCrz1-C on PDA containing 0.2 mg/mL CR or 0.01% SDS, suggesting that DdaCrz1 may regulate the expression of β-1,3-glucose synthase genes but is not involved in regulating lipid metabolism. (B-D) Colony growth on CMA, PDA, and TG media was measured every 2 days until the twelfth day. (E) Conidiation on CMA medium after 14 days of cultivation. Two asterisks mean p-value < 0.01, two-tailed t-test, n = 5. ns means no significance found.

Disruption of DdaCrz1 Decreased Cell Wall Integrity
Cell wall permeability can be affected by Congo red (CR), a cell wall inhibitor that specifically binds to β-1,3-glucose, and SDS, a surfactant that removes lipids associated with the cell wall. CR and SDS were applied to test the involvement of DdaCrz1 in maintaining cell wall integrity (CWI). The two ∆DdaCrz1 mutants were more sensitive to CR ( Figure 4A,B and Table S6), but not to SDS ( Figure 4A,C and Table S6), in comparison with strain 29 and ∆DdaCrz1-C on PDA containing 0.2 mg/mL CR or 0.01% SDS, suggesting that DdaCrz1 may regulate the expression of β-1,3-glucose synthase genes but is not involved in regulating lipid metabolism.  Comparison of the growth inhibition of the WT, ΔDdaCrz1, and ΔDdaCrz1-C strains on PDA containing 0.2 mg/ml Congo red, 0.01% SDS, and 0.03 mM menadione. Two asterisks mean p-value < 0.01, two-tailed t-test, n = 5. ns means no significance found.

Involvement of DdaCrz1 in Responding to Osmotic and Metal Ion Stresses
The ΔDdaCrz1 mutants exhibited increased resistance to osmotic stresses on PDA containing 0.2 M NaCl, 0.2 M KCl, and 0.5 M sorbitol ( Figure 5A-D; Table S7). However, strain 29, ΔDdaCrz1-C, and ΔDdaCrz1 mutants showed similar susceptibility to 0.03 mM menadione ( Figure 4A,D and Table S8). ΔDdaCrz1 exhibited increased tolerance to Ca 2+ ,

Involvement of DdaCrz1 in Responding to Osmotic and Metal Ion Stresses
The ∆DdaCrz1 mutants exhibited increased resistance to osmotic stresses on PDA containing 0.2 M NaCl, 0.2 M KCl, and 0.5 M sorbitol (Figure 5A-D; Table S7). However, strain 29, ∆DdaCrz1-C, and ∆DdaCrz1 mutants showed similar susceptibility to 0.03 mM menadione ( Figure 4A,D and Table S8). ∆DdaCrz1 exhibited increased tolerance to Ca 2+ , Mg 2+ , Li + , and Zn 2+ (Figure 5E-I and Table S9) but could not grow under Mn 2+ stress ( Figure 5J,K and Table S10). Crz1 in yeast has been documented to have high tolerance to Ca 2+ and Mn 2+ via the calcineurin-dependent induction of PMC1 and PMR1, respectively [10], indicating that DdaCrz1 may control the expression of the D. dactyloides gene homologous to PMR1.  Two asterisks mean p-value < 0.01, two-tailed t-test, n = 5. ns means no significance found.

Trap Formation and Ring Cell Inflation Affected by the Loss of DdaCrz1
Capturing nematode is an essential part of the NTF lifestyle. The numbers of traps formed by the ∆DdaCrz1 mutants after introducing C. elegans for 16 h and 24 h were 70% (16 h) and 58% (24 h), respectively ( Figure 6A,B and Table S11)compared with those formed by strain 29. The trap number was restored in ∆DdaCrz1-C. The constricting ring inflation rates of the two ∆DdaCrz1 mutants were lower than that of the WT at 16 h (3.3% vs. 5.3%) and 24 h (10.8% vs. 16.1%) after introducing C. elegans ( Figure 6C,D and Table S12). There were no significant differences between ∆DdaCrz1-C and strain 29 ( Figure 6).

Trap Formation and Ring Cell Inflation Affected by the Loss of DdaCrz1
Capturing nematode is an essential part of the NTF lifestyle. The numbers of traps formed by the ΔDdaCrz1 mutants after introducing C. elegans for 16 h and 24 h were 70% (16 h) and 58% (24 h), respectively ( Figure 6A,B and Table S11)compared with those formed by strain 29. The trap number was restored in ΔDdaCrz1-C. The constricting ring inflation rates of the two ΔDdaCrz1 mutants were lower than that of the WT at 16 h (3.3% vs. 5.3%) and 24 h (10.8% vs. 16.1%) after introducing C. elegans ( Figure 6C,D and Table  S12). There were no significant differences between ΔDdaCrz1-C and strain 29 ( Figure 6).

Discussion
Calcium ions, as second messengers, regulate a wide variety of cellular and developmental processes in eukaryotes. The calcium signaling pathway in fungi has been well characterized and is involved in growth, conidiation, thermo-tolerance, survival under ultraviolet light and oxidative stress, and sexual development [40]. For NTF, Ca 2+ /calmodulin-dependent protein kinases (CaMKs) and AoFIG_1, a member of the low-affinity Ca 2+ transport system (LACS), have been shown to be involved in the growth, conidiation, stress response, and predatory ability of A. oligospora [24,25]. Disruption of AoFIG_1 caused 90% reduction in trap formation, while deletion of AoFIG_2 reduced the vegetative growth rate by up to 44% and led to the disappearance of traps and conidia [25]. Deletion of five orthologs of CaMKs in A. oligospora also reduced the ability to produce conidia and traps [24]. The role of Crz1, a gene under the control of calcineurin, in D. dactyloides was investigated in this study. The deletion of DdaCrz1 not only impaired the growth, conidiation, and cell wall integrity but also interfered with the trap formation and ring cell inflation, supporting the involvement of the Ca 2+ signaling in nematode trapping.
The Crz1 protein, a C 2 H 2 -type transcription factor controlled by Ca 2+ /calmodulin, is conserved among fungi and has been suggested to control the expression of glucan synthase, which regulates morphological and physiological traits. Consistent with previous reports on B. cinerea, M. oryzae, and A. fumigatus [17][18][19], the disruption of DdaCrz1 led to significant defects in the growth, conidiation, and cell wall integrity of D. dactyloides.
Crz1 induces the expression of multiple genes responsive to ion stress and other stimulations in fungi [41,42]. The deletion of DdaCrz1 made the mutant more susceptible to Mn 2+ , a trait observed in the Crz1 mutants of S. cerevisiae and A. fumigatus, but not in the mutant of B. cinerea and M. grisea [10,17,18,43]. The ∆DdaCrz1 mutants displayed increased resistance to Na + , K + , Mg 2+ , Li + , and Zn 2+ stresses and the sensitivity to these cations of the crz1 mutants was complex among different fungi [10,[17][18][19]43], which suggested that the exact role of Crz1 may be species-specific. Interestingly, the ∆DdaCrz1 mutants exhibited reduced hypersensitivity to Ca 2+ compared to strain 29, which is opposite to what has been observed in other fungi, such as S. cerevisiae, A. fumigatus, M. oryzae, and B. cinerea. The difference might be due to the predatory lifestyle of nematode-trapping fungi.
The constricting ring (CR), a sophisticated trapping structure of NTF, mechanically captures nematodes by instantly inflating the ring cells when a vermiform nematode enters the ring cavity created by three ring cells. The mechanism underpinning ring constriction is poorly understood. Previous studies showed that applying inhibitors of calmodulin, calcium channels, and calcium pumps could decrease the percentages of inflated CRs in D. dactyloides [26]. Similar results were obtained when this pathway in D. stenobrocha, another constricting ring-forming fungus related to D. dactyloides, was disrupted. Chelation of Ca 2+ using EGTA and the inhibition of calmodulin and calcium channels using trifluoperazine and ruthenium red/LaCl 3 (two inhibitors of calcium channels) reduced the CR inflation rates of D. stenobrocha in a dosage-dependent manner, which indicated that Ca 2+ was involved in regulating the inflation process (work in progress). The effect of DdaCrz1 on the formation and inflation of constricting rings appeared to be significant (p < 0.05), but we cannot rule out the possibility that the reduced formation and inflation of constricting rings was simply due to the slower growth rate of the ∆DdaCrz1 mutants.
Supplementary Materials: The following supporting information can be downloaded at: https:// www.mdpi.com/article/10.3390/jof8070750/s1, Table S1: The primers used in this study, Figure S1: The PCR products corresponding to the DdaCrz1 5 and 3 flanking regions, Figure S2: PCR analysis of 96 ATMT transformants, Figure S3: PCR analysis of 48 ATMT transformants, Table S2: Colony diameter (mm) of the WT and mutants strains on CMA,  Table S11: Constricting ring number of D. dactyloides after induction by nematodes, Table S12: Constricting ring inflation number of D. dactyloides after induction by nematodes.
Author Contributions: X.L., M.X. and X.Z. designed the study; X.Z. and Y.F. performed the research; X.Z. analyzed the data; X.Z. and S.W. wrote the manuscript; X.L., S.K. and M.X. edited the manuscript. All authors have read and agreed to the published version of the manuscript.