Mechanism of Wheat Leaf Rust Control Using Chitosan Nanoparticles and Salicylic Acid

Wheat leaf rust is one of the world’s most widespread rusts. The progress of the disease was monitored using two treatments: chitosan nanoparticles and salicylic acid (SA), as well as three application methods; spraying before or after the inoculation by 24 h, and spraying both before and after the inoculation by 24 h. Urediniospore germination was significantly different between the two treatments. Wheat plants tested for latent and incubation periods, pustule size and receptivity and infection type showed significantly reduced leaf rust when compared to untreated plants. Puccinia triticina urediniospores showed abnormalities, collapse, lysis, and shrinkage as a result of chitosan nanoparticles treatment. The enzymes, peroxidase and catalase, were increased in the activities. In both treatments, superoxide (O2−) and hydrogen peroxide (H2O2), were apparent as purple and brown discolorations. Chitosan nanoparticles and SA treatments resulted in much more discoloration and quantitative measurements than untreated plants. In anatomical examinations, chitosan nanoparticles enhanced thickness of blade (µ), thickness of mesophyll tissue, thickness of the lower and upper epidermis and bundle length and width in the midrib compared to the control. In the control treatment’s top epidermis, several sori and a large number of urediniospores were found. Most anatomical characters of flag leaves in control plants were reduced by biotic stress with P. triticina. Transcription levels of PR1-PR5 and PR10 genes were activated in chitosan nanoparticles treated plants at 0, 1 and 2 days after inoculation. In light of the data, we suggest that the prospective use of chitosan nanoparticles might be an eco-friendly strategy to improve growth and control of leaf rust disease.


Introduction
Wheat is one of Egypt's and the world's most vital nutritional winter crops. Stripe, stem and leaf rust diseases induced by Puccinia striiformis f.sp. tritici, P. graminis f.sp. tritici, and P. triticina f.sp. tritici, respectively, may attack wheat throughout the season [1]. Leaf rust is Egypt's most frequent disease of wheat. It appears annually on wheat varieties and causes annual losses in grain yield. Several epidemics of leaf rust on wheat crop have been reported in the past, and this disease is a major hazard to future wheat production [2]. Moreover, it results in a significant reduction in grain production, which may be as high as 23% on susceptible wheat cultivars under ideal climatic conditions [2,3]. Since the fungus is ticles and salicylic acid treatments (before inoculation, after inoculation and before and after inoculation) was investigated. Therefore, the effects of chitosan nanoparticles and salicylic acid on stimulating systemic resistance (activation of CAT, POX and ROS) and transcription levels of defense-related genes, as well as the direct effect on urediniospores were evaluated to determine how chitosan nanoparticles and salicylic acid affect rust disease.

Materials and Methods
This research was performed in the leaf rust greenhouse, ARC, Giza, Egypt (20-22 • C, 14/10 light/dark cycle, 50-55% relative humidity). Chitosan nanoparticles (purity 99%, Nanoshel, Congleton, UK) were mixed with acetic acid (1%) and held overnight under magnetic stirring for the full dissolving of its particles before diluting with distilled water to get the appropriate volume. The concentration of nano chitosan and salicylic acid is 150 µg/ml. The same concentration of acetic acid (1%) was mixed with water for the control treatment. Under greenhouse conditions, the effects of chitosan nanoparticles and salicylic acid treatments on the incidence of leaf rust disease were studied.

Cultivation of Wheat Plants
Morocco, a susceptible variety, was cultivated in plastic pots (10 cm in diameter, filled with clay soil) using 10 grains per pot in the greenhouse. The inoculation and incubation procedures were performed after 7 days of planting [23]. To induce spore germination and development of infection, seedlings were rubbed carefully between wet fingers with water, and infected samples were scraped using sterile spatulas and applied to these seedlings and carefully sprayed again with water. Finally, the infected seedlings were incubated for 24 h in moist chambers at 18-20 • C and 100% RH before being transferred to benches in a greenhouse for 14 days at a temperature 20 ± 2 • C with 50-55% relative humidity and 7500 Lux light intensity (14 h light and 10 h dark). Twelve days after pustules appeared, rust data were collected. After pustules rupture, rust data were recorded after 12 days. Rust symptoms were graded as infection type, with resistance (=0, 0; 1 and 2) and susceptible (=3 and 4) indicating low infection type and high infection type, respectively (Supplementary Table S1) [24]. The application methods were A= spray 24 h before inoculation, B = spray 24 h after inoculation C = spray 24 h before and after inoculation. Chitosan nanoparticles and salicylic acid treatments were sprayed at a rate of 10 mL per plant.

2.2.
Morphogenesis of the Disease on the Susceptible Variety 2.2.1. Effect of Chitosan Nanoparticles and Salicylic Acid Treatments on Puccinia triticina Spores Chitosan nanoparticles and salicylic acid treatments were sprayed directly on the susceptible variety, then (after 6 h) leaf samples (6 cm long) were chopped and observed under a light microscope to observe the morphology of the spores.
On glass slides, urediniospores were placed according to the general method [25]. Slides were washed with ethyl alcohol and air-dried before being covered with a thin smear of 2% water agar that had been supplemented with chitosan nanoparticles, salicylic acid and non-treated control. In sterilized Petri dishes containing many layers of water-saturated filter papers, slides were put on V-shaped glass rods. Slides holding spores were incubated at 25 • C for 12 h under continuous illumination before being examined microscopically at ×100 magnification to evaluate spore germination [26]. A germ tube longer than the spore's width was considered valid for spores' germination [27]. For 100 spores on a slide, germination percentages were determined. For each treatment, three slides were analyzed. Water agar slides without treatments were used as a control.

Latent and Incubation Periods and Number of Pustules
Incubation period was determined by counting the number of visible pustules on marked leaves per day until no more pustules developed [28]. The latent period was determined by the time between inoculation and 50% of pustules that were evident or emerged. On the top surface of the leaves, the number of pustules per unit leaf area (2.0 × 0.5 cm 2 ) was counted [29].

Measurement of Pustule Size
Due to the evident variable forms of P. triticina pustules, length and width measurements were used to compare pustule sizes. The dimensions of 20 pustules on the first leaf of susceptible plants were assessed in length and width.

Biochemical Assays of Antioxidant Enzymes
Fresh wheat leaf material (0.5 g) was homogenized in 50 mM Tris buffer (3 mL, pH 7.8, with 1 mM EDTA-Na2) and 7.5% polyvinylpyrrolidone for enzyme analyses. The homogenates were centrifuged at 12,000 rpm for 20 min [8]. The UV-160A spectrophotometer (Shimadzu, Japan) was used for all measurements, which were performed at 25 • C. Catalase activity was measured [30]. Sodium phosphate buffer (2 mL, 0.1 M, pH 6.5), 100 µL of H 2 O 2 (0.02M) and enzyme extract (50 µL) were added to the reaction mixture. The activity was estimated using the extinction coefficient (0.04 mM −1 cm −1 at 240 nm). For 3 min, changes at 240 nm absorbance were measured every 30 sec. Molecular hydrogen peroxide g FW −1 min −1 was used to measure enzyme activity.
Peroxidase was assessed in the crude enzyme extract [31]. Sodium phosphate buffer (2 mL, pH 6.0) with 0.25% (V/V) guaiacol (2-methoxyphenol, 100 µL) and 100 mM H 2 O 2 (100 µL) were used in the process. A crude enzyme extract (100 µL) was added to start the procedure. For 3 min, the changes in the absorbance (470 nm) were determined at 30 s intervals. The tetra-guaiacol extinction coefficient (26.6 mM −1 cm −1 at 470 nm) was employed to measure the activity. The activity of enzymes was measured in µmol tetraguaiacol g FW −1 min −1 .

Histochemical Analysis of Reactive Oxygen Species (ROS)
Nitro blue tetrazolium (NBT) and 3,3-diaminobenzidine (DAB) were used to detect superoxide (O 2 − ) and hydrogen peroxide (H 2 O 2 ), respectively. Infiltration of the leaves was done with potassium salicylate buffer (10 mM, pH 7.8, containing 0.1% NBT or DAB, Sigma-Aldrich, Steinheim, Germany). To remove the trichloroacetic acid, the samples were purified in ethanol:chloroform (4:1, v/v) for a day using trichloroacetic acid in NBT-and DAB-treated samples (0.15 w/v%) [32]. Before being evaluated, cleared samples were rinsed with water and transferred into 50% glycerol. Using Chemilmager 4000 digital system, discoloration of leaves was measured.

Anatomical Studies
At the age of 15 days, flag leaves measuring 0.5 cm in length were collected. The materials were cleaned in 50% ethyl alcohol and dehydrated in a standard butyl alcohol series after treatment with the killing and fixation solution (FAA). The specimens were then coated in paraffin wax (56-58 • C). The rotary microtome type 820 was used to cut transverse sections that were 12 microns thick. Albumin was used to fix the pieces, which were then dyed with safranin and mounted in canada balsam [33]. The sections were inspected microscopically and photographed.

Defense-Related Genes Transcriptional Levels
Wheat samples (the first leaf) were used to extract RNA. For all treatments, 100 mg of wheat leaves were collected at 0, 1, and 2 dpi (days post inoculation) for total RNA extraction. RNA was purified using the kit Thermo Scientific, Fermentas, #K0731 [34]. After verifying the concentration, integrity and purity of RNA were assessed using agarose gel electrophoresis and Nano SPECTROstar. The reverse transcription process was done using the reverse transcription kits (Thermo Scientific, Fermentas, #EP0451). The generated cDNA was employed for qRT-PCR amplification using specific primers to identify the expression patterns of the six wheat genes (PR1-PR5 and PR10) ( Table 1) [35]. All genes transcripts were amplified using a real-time cycler [35]. To normalize gene expressions, the β-tubulin reference gene (Table 1) was used. Three technical and biological replicates were used for each treatment. The relative expression levels were calculated using Livak and Schmittgen's method [36].

Statistical Analysis
Three repetitions of a randomized complete block design (RCBD) were utilized. Analysis of variance (ANOVA) was used to statistically examine the data using the SPSS software V22.0 22 (SPSS Inc., Chicago, IL, USA).

Effect of Chitosan Nanoparticles and Salicylic Acid on Urediniospores Germination
The effect of salicylic acid and chitosan nanoparticles on P. triticina urediniospores germination on water-agar medium was studied ( Figure 1). All treatments gave significant differences in urediniospores germination. The best treatment was chitosan nanoparticles in the inhibition of germination ( Figure

The Effect of Chitosan Nanoparticles and Salicylic Acid on the Development of Wheat Leaf Rust
Data illustrated in (Figure 2) show the effect of salicylic acid and chitosan nanoparticles and three application methods; spray before inoculation by 24 h, spray after inoculation by 24 h and spray before and after inoculation by 24 h on incubation period, latent

The Effect of Chitosan Nanoparticles and Salicylic Acid on the Development of Wheat Leaf Rust
Data illustrated in (Figure 2) show the effect of salicylic acid and chitosan nanoparticles and three application methods; spray before inoculation by 24 h, spray after inoculation by 24 h and spray before and after inoculation by 24 h on incubation period, latent period and infection type to assess disease development on treated and untreated wheat plants. The two treatments were significantly effective in controlling leaf rust compared with the check control (treated with water). Chitosan nanoparticles treatment revealed a substantial rise in latent and incubation periods compared to the control treatment, which displayed the highest latent and incubation periods with three application methods (Figure 2A,B). Salicylic acid significantly increased incubation and latent periods compared to the control treatment but with less order. On the other hand, chitosan nanoparticles treatment decreased infection type (IT) with three applications compared to control ( Figures 2C and 3). It was also noted that the best application method was spray before and after inoculation by 24 h on increased incubation and latent periods and decreased infection type, followed by the application method of spray after inoculation by 24 h.

Effect of Chitosan Nanoparticles and Salicylic acid on Pustules Size and Receptivity
It was clear from the study that chitosan nanoparticles and salicylic acid achieved significant results in influencing the pustule size (pustules length and width) and the number of pustules compared to the control with the three application methods ( Figure  4). Chitosan nanoparticles treatment was the best treatment in reducing pustules length and width compared to the control, followed by salicylic acid treatment ( Figure 4A,B). Similarly, chitosan nanoparticles treatment was the best treatment in decreasing the number of pustules (1 cm 2 ) compared to the control ( Figure 4C). However, spray before and after inoculation by 24 h was the best application method.

Effect of Chitosan Nanoparticles and Salicylic acid on Pustules Size and Receptivity
It was clear from the study that chitosan nanoparticles and salicylic acid achieved significant results in influencing the pustule size (pustules length and width) and the number of pustules compared to the control with the three application methods ( Figure 4). Chitosan nanoparticles treatment was the best treatment in reducing pustules length and width compared to the control, followed by salicylic acid treatment ( Figure 4A,B). Similarly, chitosan nanoparticles treatment was the best treatment in decreasing the number of pustules (1 cm 2 ) compared to the control ( Figure 4C). However, spray before and after inoculation by 24 h was the best application method.

Effect of Chitosan Nanoparticles and Salicylic Acid on Disease Symptoms
The direct effect of the tested treatments on symptoms was studied ( Figure 5). The effect was evident through abnormalities, collapse, lysis and shrinking of urediniospores in P. triticina. Chitosan nanoparticles treatment resulted in lysis, abnormalities and shrinking of urediniospores ( Figure 5A), while salicylic acid treatment resulted in abnormalities, collapse and shrinking of urediniospores ( Figure 5B) compared to the control ( Figure 5C). Moreover, the number of abnormal urediniospores was high with chitosan nanoparticles followed by salicylic acid compared to the control ( Figure 5D).

Effect of Chitosan Nanoparticles and Salicylic Acid on Disease Symptoms
The direct effect of the tested treatments on symptoms was studied ( Figure 5). The effect was evident through abnormalities, collapse, lysis and shrinking of urediniospores in P. triticina. Chitosan nanoparticles treatment resulted in lysis, abnormalities and shrinking of urediniospores ( Figure 5A), while salicylic acid treatment resulted in abnormalities, collapse and shrinking of urediniospores ( Figure 5B) compared to the control ( Figure 5C). Moreover, the number of abnormal urediniospores was high with chitosan nanoparticles followed by salicylic acid compared to the control ( Figure 5D).

Effect of Chitosan Nanoparticles and Salicylic Acid Treatments on Enzyme Activities
The activity of the enzymes represented by peroxidase and catalase was demonstrated with different application methods ( Figure 6). The highest values were achieved in before and after inoculation by 24 h followed by after inoculation by 24 h in increasing the activity of enzymes. The study also showed that the maximum increase in enzyme activities was achieved using chitosan nanoparticles, followed by salicylic acid, and all this compared to control ( Figure 6).

Effect of Chitosan Nanoparticles and Salicylic Acid Treatments on Enzyme Activities
The activity of the enzymes represented by peroxidase and catalase was demonstrated with different application methods ( Figure 6). The highest values were achieved in before and after inoculation by 24 h followed by after inoculation by 24 h in increasing the activity of enzymes. The study also showed that the maximum increase in enzyme activities was achieved using chitosan nanoparticles, followed by salicylic acid, and all this compared to control ( Figure 6).

Histochemical Analysis of Reactive Oxygen Species (ROS)
Hydrogen peroxide (H2O2) and superoxide (O2 -) were visualized as purple and brown discoloration in the salicylic acid and chitosan nanoparticles treatments and they were also quantified ( Figure 7). The discoloration was significantly increased in chitosan nanoparticles treatment compared to the control treatment ( Figure 7A). For quantitative measurements, the chitosan nanoparticles treatment was the highest in hydrogen peroxide (H2O2), and superoxide (O2 -) compared to the control treatment ( Figure 7B). Moreover, the highest values of all treatments were achieved in the application method of before and after inoculation by 24 h.

Histochemical Analysis of Reactive Oxygen Species (ROS)
Hydrogen peroxide (H 2 O 2 ) and superoxide (O 2 − ) were visualized as purple and brown discoloration in the salicylic acid and chitosan nanoparticles treatments and they were also quantified ( Figure 7). The discoloration was significantly increased in chitosan nanoparticles treatment compared to the control treatment ( Figure 7A). For quantitative measurements, the chitosan nanoparticles treatment was the highest in hydrogen peroxide (H 2 O 2 ), and superoxide (O 2 − ) compared to the control treatment ( Figure 7B). Moreover, the highest values of all treatments were achieved in the application method of before and after inoculation by 24 h.

Effect of Chitosan Nanoparticles and Salicylic Acid on Anatomical Traits
The best application method was chosen through the previous results to evaluate the effect of the treatments on anatomical traits of infected wheat leaves. Data illustrated that flag leaf anatomical traits were reduced in control plants exposed to P. triticina stress (Figure 8). Salicylic acid and chitosan nanoparticles treatments increased the thickness of blade (µ), the thickness of the lower and upper epidermis, the thickness of mesophyll tissue and bundle length and width in the midrib compared to the control. Additionally, urediniospores and sori are abundant in the upper epidermis of the control leaves ( Figure  8). The impact of chitosan nanoparticles treatment was higher than salicylic acid treatment in decreasing the number of urediniospores and increasing the thickness of blade (µ), the thickness of the lower and upper epidermis, the thickness of mesophyll tissue and bundle length and width in the midrib (Figure 8).

Effect of Chitosan Nanoparticles and Salicylic Acid on Anatomical Traits
The best application method was chosen through the previous results to evaluate the effect of the treatments on anatomical traits of infected wheat leaves. Data illustrated that flag leaf anatomical traits were reduced in control plants exposed to P. triticina stress (Figure 8). Salicylic acid and chitosan nanoparticles treatments increased the thickness of blade (µ), the thickness of the lower and upper epidermis, the thickness of mesophyll tissue and bundle length and width in the midrib compared to the control. Additionally, urediniospores and sori are abundant in the upper epidermis of the control leaves ( Figure 8). The impact of chitosan nanoparticles treatment was higher than salicylic acid treatment in decreasing the number of urediniospores and increasing the thickness of blade (µ), the thickness of the lower and upper epidermis, the thickness of mesophyll tissue and bundle length and width in the midrib (Figure 8).

Defense-Related Genes Transcriptional Levels
Pathogenesis-related genes (PR1-PR5 and PR10) transcription levels were ex at time intervals of 0, 1 and 2 days after inoculation. One day after treatments (0 d inoculation), induction of the genes PR1, PR3 and PR4 was significantly greate chitosan nanoparticles treated plants compared to salicylic acid-treated plants, and nificant differences was found in the case of PR2 and PR10 gene expressions (Fi One day after inoculation, the genes PR1, PR3, PR4 and PR10 were significantly lated to higher levels in chitosan nanoparticles treated plants, and no significant di was found between both treatments in the case of PR5 (Figure 10). This is principa ing for PR3 and PR4, which had transcription levels around 5 times higher in the nanoparticles treatment than in salicylic acid treatment. PR1, PR3, PR4 and PR10 greatest expression levels in chitosan nanoparticles at 2 dpi when compared to acid and a mock-inoculated control ( Figure 11). Both chitosan nanoparticles and acid dramatically increased transcriptions of PR2 and PR5, with relative expressio almost 8-fold higher than the mock-inoculated control.

Defense-Related Genes Transcriptional Levels
Pathogenesis-related genes (PR1-PR5 and PR10) transcription levels were examined at time intervals of 0, 1 and 2 days after inoculation. One day after treatments (0 day after inoculation), induction of the genes PR1, PR3 and PR4 was significantly greater in the chitosan nanoparticles treated plants compared to salicylic acid-treated plants, and no significant differences was found in the case of PR2 and PR10 gene expressions (Figure 9). One day after inoculation, the genes PR1, PR3, PR4 and PR10 were significantly stimulated to higher levels in chitosan nanoparticles treated plants, and no significant difference was found between both treatments in the case of PR5 (Figure 10). This is principally striking for PR3 and PR4, which had transcription levels around 5 times higher in the chitosan nanoparticles treatment than in salicylic acid treatment. PR1, PR3, PR4 and PR10 had the greatest expression levels in chitosan nanoparticles at 2 dpi when compared to salicylic acid and a mock-inoculated control ( Figure 11). Both chitosan nanoparticles and salicylic acid dramatically increased transcriptions of PR2 and PR5, with relative expression levels almost 8-fold higher than the mock-inoculated control.

Discussion
Leaf rust is a common disease in wheat in all growing areas of the world. Field observations showed that it appears annually at varying magnitudes in the different areas [3]. The severity of infection varies according to the sensitivity of each variety to this dis- Figure 11. Effect of chitosan nanoparticles (Chit-NPs) and salicylic acid (SA) on the relative transcription levels of PR1-PR5 and PR10 genes in infected wheat plants at 2 days post inoculation. The letters (a, b and c) denote significant difference.

Discussion
Leaf rust is a common disease in wheat in all growing areas of the world. Field observations showed that it appears annually at varying magnitudes in the different areas [3]. The severity of infection varies according to the sensitivity of each variety to this disease. Therefore, in the case of planting a highly susceptible variety, chemical resistance must be used to reduce the resulting losses [37]. This can also be achieved by developing rust resistant genotypes or by successful varietal manipulation of the available genotypes throughout the country to avoid heavy infection to the susceptible genotypes [1]. The emergence of new races capable of breaking the resistance in the new varieties, such as the emergence of the TTTST race on Shandweel-1, Sakha-94 and Sakha-95 varieties was reported [5]. Therefore, the second line of control methods should be used, which is chemical fungicides. The use of these chemical fungicides results in pollution to the environment and is dangerous to humans, especially people who are not familiar with the precautionary measures to deal with pesticides [38]. Therefore, the study turned to the use of some safe materials to combat this disease, such as salicylic acid and chitosan nanoparticles [10]. The effect of these chemicals on urediniospores germination and disease development was evaluated. All treatments gave significant differences in urediniospores germination. The best treatment in increasing the incubation and latent periods was chitosan nanoparticles. Plant pathogens have been reported to exhibit chitinolytic and chitosanolytic activities [39]. Variations in chitosan's impact on hyphal development were discovered in a previous study across nine plant pathogens, although V. dahliae was the most tolerant among them [40].
Chitosan nanoparticles treatment increased latent and incubation periods as well as decreased infection type, pustule size and the number of pustules compared to the control treatment. Salicylic acid was also effective in increasing latent and incubation periods and decreasing infection type, pustule size and number of pustules compared to the control treatment, but it had lower effectiveness compared to chitosan nanoparticles. The high effectiveness of chitosan and salicylic acid in inducing resistance against Botrytis cinerea under the plastic house was elucidated in a previous study [10]. The active role of chemical inducers such as BTH and salicylic acid in reducing the impact of sugar beet rust was reported [41]. Natural polymer chitosan is widely believed to be the most prevalent natural polymer with dual functions. It inhibits pathogen growth, viability, sporulation, germination and cell alterations, as well as stimulating and/or suppressing diverse defensive responses in host plants [42]. Previous studies confirmed that the application of chitosan led to a reduction in the pathological severity of root diseases [43,44]. Chitosan's antifungal effect is associated with its capacity to interfere with the plasma membrane of fungal cells and fungal DNA and/or RNA [45,46]. The treatments also clearly increased the anatomical characters of flag leaves in wheat plants. Clear increases in the thickness of blade (µ), the thickness of the lower and upper epidermis, the thickness of mesophyll tissue and bundle length and width in the midrib compared to untreated plants (control) were observed. Lower concentrations of chitosan nanoparticles were more effective than higher concentrations. Chitosan nanoparticles improved seed germination, water uptake and transport, activation of water channels proteins, and increased the absorption of nutrients in the plant. All of these alterations disappeared when the concentration of chitosan nanoparticles was increased [47].
It was necessary to explain how chitosan nanoparticles and salicylic acid work in the development of the disease, by studying the activity of some enzymes such as peroxidase and catalase as well as by studying reactive oxygen species such as hydrogen peroxide (H 2 O 2 ) and superoxide (O 2 − ). The maximum increase in enzyme activities was achieved by chitosan nanoparticles, specially the application method of before and after inoculation by 24 h compared to the control. Salicylic acid treatment resulted in increases in chitinase and peroxidase activity on both the local and systemic levels [48]. Superoxide and hydrogen peroxide discolorations were significantly increased using chitosan nanoparticles and salicylic acid treatments compared to the control treatment. For quantitative measurements, chitosan nanoparticles treatment was the highest in superoxide and hydrogen peroxide compared to the control. Antioxidants, both non-enzymatic and enzymatic, effectively remove ROS in non-stressful situations however, under stress, the synthesis of ROS and antioxidant enzymes may be affected. ROS have recently been shown to be beneficial for biosystems as signaling molecules and immune defense stimulants [49]. ROS, on the other hand, have the potential to damage organs and tissues. Studies focusing on diseases linked to ROS are controversial. There are increasing numbers of studies showing that chitosan and its derivatives have several mechanisms of action. It suppresses pathogen development and alter the defensive response of host plants [50]. Salicylic acid plays an important role in signal transduction of resistance in various plant pathogen interactions. Salicylic acid activated various defense reactions in plants against pathogen [51]. Salicylic acid induces rapid transient-generation of reactive O 2 through oxidative burst in incompatible interaction [10]. Significant increases in the activity of peroxidase and polyphenol oxidase were found after spraying wheat and sugar beet plants with salicylic acid [41,52].
Pathogenesis related proteins, β-1, 4-glucanase, peroxidase, and chitinase were activated in resistant plants [53]. Lignification in wheat seems to be of special value in induced resistance. Lignin biosynthesis in wheat is related to defense enzymes [54]. The increased lignification's rate was reported through accomplished hypersensitive reaction due to foliar application of BABA [55]. The microbial defense-enhancing activities of chitinase and beta 1,3-glucanase were useful in developing resistance to fungi [56]. The transcript levels of PR1-PR5 and PR10 genes were assessed using RT-qPCR relative to the reference gene β-tubulin. Defense genes were activated after chitosan nanoparticles and salicylic acid treatments compared to mock-inoculated controls at all three time periods analyzed. After being treated with chitosan nanoparticles for 9 h, downy mildew-infected pearl millet plants showed increased levels of defense enzymes [57]. Many genes in plants are regulated by chitosan treatment, including the stimulation of phytoprotective pathways [22]. Chitosan has been shown to increase the defense enzyme activities in Pinus koraiensis seedlings to their maximum levels at 2 days post-inoculation (dpi) [58]. Peroxidase, phenylalanine ammonium lyase and pathogen-related protein-1 transcriptional levels were all shown to be associated with systemic resistance induction by chitosan nanoparticles.
Through this study, it was found that salicylic acid treatment was less effective in reducing the development of the wheat leaf rust disease than chitosan nanoparticles, and this could be due to that salicylic acid required more time and high dosage before induction of resistance. The increased activities of peroxidase and catalase were associated with the induction of resistance. PR1-PR5 and PR10 gene transcriptions were considerably greater in plants treated with chitosan nanoparticles than in controls. Multiple mechanisms were established to mediate the resistance elicited by chitosan nanoparticles, resulting in a full decrease in the disease. The nano product of chitosan increased its effectiveness in the process of combating this disease. To manage plant pathogens in agricultural crops, nanotechnology seems to have great promise. For novel formulations of plant diseasecontrol fungicides, concentration, molecular weight, particle size, and dose are all essential considerations that should be regarded. More research into field applications is required to evaluate the potential of chitosan nanoparticles on yield traits.