Morphology, Molecular Identification, and Pathogenicity of Two Novel Fusarium Species Associated with Postharvest Fruit Rot of Cucurbits in Northern Thailand

Fruit rot of cucurbits caused by several pathogenic fungi has become an important postharvest disease worldwide. In 2022, fruit rot on watermelon (Citrullus lanatus) and muskmelon (Cucumis melo) was observed during the postharvest storage phase in the Chiang Mai and Phitsanulok Provinces of northern Thailand. These diseases can lead to significant economic losses. This present study was conducted to isolate the causal agent of fungi in lesions of fruit rot. A total of four fungal isolates were obtained, of which two isolates (SDBR-CMU422 and SDBR-CMU423) were obtained from rot lesions of watermelons, while the remaining isolates (SDBR-CMU424 and SDBR-CMU425) were obtained from rot lesions of muskmelons. All fungal isolates were identified using both morphological characteristics and molecular analyses. Morphologically, all isolated fungal isolates were classified into the genus Fusarium. Multi-gene phylogenetic analyses of a combination of the translation elongation factor 1-alpha (tef-1), calmodulin (cam), and RNA polymerase second largest subunit (rpb2) genes reveled that four fungal isolates belonged to the Fusarium incarnatum–equiseti species complex and were distinct from all other known species. Thus, we have described them as two new species, namely F. citrullicola (SDBR-CMU422 and SDBR-CMU423) and F. melonis (SDBR-CMU424 and SDBR-CMU425). A full description, illustrations, and a phylogenetic tree indicating the position of both new species have been provided. Moreover, pathogenicity tests were subsequently performed and the results showed that F. citrullicola and F. melonis caused symptoms of fruit rot on inoculated watermelon and muskmelon fruits, respectively. Notably, this outcome was indicative of the symptoms that appeared during the postharvest storage phase. To our knowledge, two new pathogenic fungi, F. citrullicola and F. melonis, are new causal agents of watermelon and muskmelon fruit rot, respectively. Importantly, these findings provide valuable information for the development of effective strategies for the monitoring and prevention of these diseases.


Introduction
Watermelon [Citrullus lanatus (Thunb.) Mats. & Nakai] and muskmelon (Cucumis melo L.) are both cucurbit species that belong to the family Cucurbitaceae [1]. Both crops are extensively cultivated in temperate, subtropical, and tropical regions throughout the world [2,3]. Both fruits have been described as healthy food choices for human consumption [4,5]. The primary nutritional components found in watermelon and muskmelon fruits include amino acids, ascorbic acid, β-carotene, carbohydrates, fatty acids, flavonoids, minerals, potassium, sugars, vitamins, and a number of bioactive compounds [2,[5][6][7][8]. They also possess beneficial medicinal properties such as analgesic, anticancer, anti-inflammatory, antimicrobial, antioxidant, antiulcer, and hepatoprotective properties [9,10]. More interestingly, the watermelon fruit is a great natural source of lycopene and also principally contains about 93% water [2]. In 2020, China is known to be the largest producer of watermelon and melon (including muskmelon) fruits in the global market, followed by Turkey, India, and Iran [11]. In Southeast Asia, Indonesia led in melon production in 2020, followed by the Lao People's Democratic Republic and the Philippines [11]. In addition, Vietnam led Southeast Asia in watermelon production in 2020, followed by Indonesia, the Lao People's Democratic Republic, and Thailand [11]. Currently, muskmelon has emerged as one of the economically important crops in Thailand.
Several diseases caused by microorganisms (bacteria, fungi, and viruses) can have negative effects on cucurbit plants throughout both the growing period and the postharvest period [4,[12][13][14]. Fruit rot disease is one of the most typical preharvest and postharvest diseases of cucurbit fruits in Thailand and worldwide [4,[15][16][17][18][19]. This disease has caused huge losses through reductions in harvest yields and lowered standards of quality, both of which can have a significant negative economic impact [20][21][22]. Previous studies indicate that the fungal species belonging to the genera Alternaria, Didymella, Epicoccum, Fusarium, Lasiodiplodia, Myrothecium, Penicillium, Phomopsis, Phytophthora, Pythium, Rhizoctonia, and Sclerotium have been reported as causal agents of fruit rot in cucurbits (cantaloupes, cucumbers, melons, pumpkins, squashes, and watermelons) [4,15,17,[23][24][25][26]. The demand for watermelon and muskmelon fruits has risen due to the rapid growth of the world's population and an increased interest in pursuing a healthy lifestyle. Therefore, plantation areas dedicated to the cultivation of both watermelon and other melon plants have increased significantly. Conversely, the prevalence and severity of some fungal-based diseases have also increased when plants have been grown in sub-optimal locations [4,27,28]. In Thailand, the major areas for watermelon and melon cultivation are located in the northern region of the country, including Chiang Mai, Kamphaeng Phet, Phayao, Phichit, Phitsanulok, and Sukhothai Provinces. However, there have been relatively few studies on incidences of postharvest fruit rot of watermelon and muskmelon in Thailand. In 2022, fruit rot caused by fungi was observed on watermelon and muskmelon during the postharvest storage phase in Chiang Mai and Phitsanulok Provinces, respectively. The incidence of this disease ranged from 20 to 30% according to the number of fruits in each pallet box (100 fruits per pallet box). Therefore, this study aimed to isolate the causal fungal agents of these fruit rot diseases. The obtained fungi were identified and described by their morphological characteristics and through multigene phylogenetic analysis. Subsequently, a pathogenicity test was subsequently performed and Koch's postulates were employed to evaluate the asymptomatic fruits of watermelon and muskmelon using the isolated fungi.

Sample Collection
Fruit rot disease on watermelon (C. lanatus) and muskmelon (C. melo) fruits was observed during the postharvest storage phase in Chiang Mai and Phitsanulok Provinces of northern Thailand in 2022. Ten fruits of each symptomatic watermelon and muskmelon from different locations were randomly collected, maintained in sterile plastic boxes, and carried to the laboratory within 48 h of collection. After being transferred to the laboratory, symptomatic fruits were examined using a stereo microscope (Nikon H55OS, Tokyo, Japan) and then stored in a plastic box with wet filter paper in order to stimulate sporulation.

Fungal Isolation
Fruits samples were processed for the isolation of fungal causal agents. Causal fungi were isolated from lesions using a single conidial isolation on 1.0% water agar containing 0.5 mg/l streptomycin under a stereo microscope according to the method described by Choi et al. [29]. The isolated plates were incubated at 25 • C in darkness for 24-48 h, and the germinated conidia were then transferred onto potato dextrose agar (PDA; Conda, Madrid, Spain) containing 0.5 mg/L streptomycin. Pure fungal isolates were deposited in the culture collection of the Sustainable Development of Biological Resources, Faculty of Science, Chiang Mai University (SDBR-CMU), Chiang Mai Province, Thailand.

Morphological Study
The morphological characteristics of fungal isolates were determined according to methods established by Crous et al. [30] and Wang et al. [31,32]. Colony characteristics, including colony morphology, pigmentation, and odor, were observed on PDA, oatmeal agar (OA; Difco, Le Pont de Claix, France), and synthetic nutrient-poor agar (SNA) after one week of incubation in the dark at 25 • C. Color notations were rated according to the color charts of Kornerup and Wanscher [33]. Micromorphological characteristics were identified using sterile water as a mounting medium under a light microscope (Nikon Eclipse Ni-U, Tokyo, Japan). Anatomical structure related to size data (e.g., chlamydospores, conidiophores, conidiogenous cells, conidia, and phialides) was based on at least 50 measurements of each structure using the Tarosoft (R) Image Frame Work program.

DNA Extraction and PCR Amplification and Sequencing
Genomic DNA of each fungal isolate was extracted from the fungal mycelia grown on PDA in darkness at 25 • C for five days using the Fungal DNA Extraction Kit (FAVORGEN, Ping-Tung, Taiwan) following the manufacturer's protocol. The translation elongation factor 1-alpha (tef-1), calmodulin (cam), and RNA polymerase second largest subunit (rpb2) genes were amplified by the polymerase chain reaction (PCR) using EF1/EF2 primers [34], CAL-228F/CAL-2Rd primers [35] and RPB2-5F2/RPB2-7cR primers [36], respectively ( Table 1). The amplification program of three genes was conducted in separate PCR reactions. The amplification process consisted of an initial denaturation step at 95 • C for 3 min, followed by 35 cycles of denaturation at 95 • C for 30 s, annealing steps at 60 • C for 50 s (tef-1), 59 • C for 30 s (cam) and 52 • C for 1 min (rpb2), and a final extension step at 72 • C for 1 min on a peqSTAR thermal cycler (PEQLAB Ltd., Fareham, UK). PCR products were checked on 1% agarose gel electrophoresis. Table 1. Details of primers and the obtained PCR product in this study.

Gene
Primer Name Primer Sequence The Obtained Length (bp)

Sequence Alignment and Phylogenetic Analyses
An analysis of the tef-1, cam, and rpb2 sequences was carried out with the use of similarity searches using the BLAST program available at NCBI (https://blast.ncbi.nlm. nih.gov, accessed on 5 August 2022). The sequences from this study, along with those obtained from previous studies and the GenBank database (with ≥60% query coverage and ≥85-100% sequence similarity) were selected and are listed in Table 2. Multiple sequence alignment was performed with MUSCLE [37] and improved where necessary using BioEdit v. 6.0.7 [38]. Phylogenetic analysis was carried out using combination datasets of tef-1, cam, and rpb2. Fusarium camptoceras CBS 193.65 and F. neosemitectum CBS 115476 within the F. camptoceras species complex (FCAMSC) were used as the outgroup. A phylogenetic tree was constructed under the maximum likelihood (ML) and Bayesian inference (BI) methods. The ML analysis was carried out using RAxML v7.0.3 on the GTRCAT model with 25 categories and 1000 bootstrap (BS) replications [39,40] via the online portal CIPRES Science Gateway v. 3.3 [41]. BI analysis was performed with MrBayes v3.2.6 software for Windows [42]. The best substitution models for BI and ML analyses were estimated using the jModelTest 2.1.10 [43] by employing the Akaike information criterion (AIC). Both ML and BI analyses were based on the GTR + I + G model. For BI analysis, six simultaneous Markov chains were run for one million generations with random initial trees, wherein every 1000 generations were sampled. A burn-in phase was employed to discard the first 2000 trees, while the remaining trees were used to construct the 50% majority-rule consensus phylogram with calculated Bayesian posterior probabilities (PP). The tree topologies were visualized in FigTree v1.4.0 [44].   Note: species obtained in this study are in bold. Superscript "T", "ET", and "NT" represents ex-type, epi-type, and neotype species, respectively. "-" represents the absence of sequence data in GenBank.

Pathogenicity Tests
Asymptomatic commercial watermelon and muskmelon fruits were carefully washed, and the surfaces were disinfected by immersion in 1.5% (v/v) sterile sodium hypochlorite solution for 5 min. They were then subsequently washed three times with sterile distilled water. The surface disinfected fruits were then air-dried at room temperature (25 ± 2 • C) for 10 min [50]. After being air-dried, a uniform wound (5 pores, 1 cm in depth and 1 mm in width) was made at the equator of each fruit using aseptic needles [4]. Conidial suspensions of all fungal isolates were prepared from each fungal culture grown on PDA at 25 • C for two weeks and suspended in sterile distilled water. The suspension was filtered through two layers of sterile cheesecloth, diluted in distilled water with 0.05% (v/v) Tween 20, and adjusted to 1 × 10 6 conidia/mL using a hemacytometer. Five hundred microliters of the conidial suspension was dropped onto the wounded fruits. Accordingly, control fruits were also wounded and treated with sterile distilled water. Each fruit was then placed in a separate sterile plastic box (26 cm × 35.5 cm × 20 cm) at conditions of 80% relative humidity. The plastic boxes were stored in a growth chamber at 25 • C under a 12 h period of light for one week. Ten replications were conducted for each treatment. The experiments were independently repeated twice. The disease severity score was employed to evaluate the specimens following the method described by Safari et al. [51] with mild (1-25%), moderate (26-50%), severe (51-75%), and very severe (76-100%) degrees of infection for the damaged fruit areas. To authenticate the causal agent, the fungi were re-isolated from the lesions following the method described by Bika and Baysal-Gurel [52].

Sample Collection and Disease Symptoms
Samples of fruit rot on specimens of watermelon (C. lanatus) and muskmelon (C. melo) were collected from postharvest storage pallet boxes located in Chiang Mai and Phitsanulok Provinces of northern Thailand, respectively. The incidence of this disease ranged from 20 to 30% according to the number of fruits in each pallet box (100 fruits per pallet box). Symptoms on watermelon were characterized by the initial presence of small light-brown spots. These spots then expanded into irregular brown spots, and the epidermal tissue was covered with white mycelia tissue (Figure 1a,b). Disease symptoms on the muskmelon started at the top and base of the fruit appearing as brown spots surrounded by a bruise margin. Eventually, white mycelial masses covered the advanced lesions (Figure 1e,f). Lesions of both the watermelon and muskmelon fruit finally became widened and merged to cover the entire fruit, causing both of the infected fruits to appear bruised, ruptured, and decayed. The internal area of decay appeared to be clearly rotten and was surrounded by water-soaked tissue (Figure 1c,d,g,h).

Fungal Isolation
A total of four fungal isolates were obtained in this study. Two fungal isolates, CMU422 and CMU423, were isolated from watermelon fruits rot collected from Chiang Mai Province and two isolates, CMU424 and CMU425, were isolated from muskmelon fruits rot collected from Phitsanulok Province. All fungal isolates were deposited at the SDBR-CMU under the accession numbers SDBR-CMU422, SDBR-CMU423, SDBR-CMU424, and SDBR-CMU425, respectively.

Morphological Study
Fungal colonies of each isolate were observed on three different agar media including PDA, OA, and SNA at 25 °C. After being incubated for one week, OA was found to be the best media by displaying the highest colony diameter of all four isolates. All four fungal isolates produced conidiophores, conidiogenous cells, chlamydospores, phialides, and conidia in all of the agar media. Based on the morphological characteristics, all fungal isolates were initially identified as belonging to the genus Fusarium [30][31][32]45]. The results obtained from morphological observation of the fungal colony and the micromorphological characters revealed that the isolate SDBR-CMU422 was similar to the isolate SDBR-CMU423, and that the isolate SDBR-CMU424 was similar to the isolate SDBR-CMU425. The fungal identification was then further confirmed through multi-gene phylogenetic analysis of a combination of the translation elongation factor 1-alpha (tef-1), calmodulin (cam), and the RNA polymerase second largest subunit (rpb2) genes.

Fungal Isolation
A total of four fungal isolates were obtained in this study. Two fungal isolates, CMU422 and CMU423, were isolated from watermelon fruits rot collected from Chiang Mai Province and two isolates, CMU424 and CMU425, were isolated from muskmelon fruits rot collected from Phitsanulok Province. All fungal isolates were deposited at the SDBR-CMU under the accession numbers SDBR-CMU422, SDBR-CMU423, SDBR-CMU424, and SDBR-CMU425, respectively.

Morphological Study
Fungal colonies of each isolate were observed on three different agar media including PDA, OA, and SNA at 25 • C. After being incubated for one week, OA was found to be the best media by displaying the highest colony diameter of all four isolates. All four fungal isolates produced conidiophores, conidiogenous cells, chlamydospores, phialides, and conidia in all of the agar media. Based on the morphological characteristics, all fungal isolates were initially identified as belonging to the genus Fusarium [30][31][32]45]. The results obtained from morphological observation of the fungal colony and the micromorphological characters revealed that the isolate SDBR-CMU422 was similar to the isolate SDBR-CMU423, and that the isolate SDBR-CMU424 was similar to the isolate SDBR-CMU425. The fungal identification was then further confirmed through multi-gene phylogenetic analysis of a combination of the translation elongation factor 1-alpha (tef-1), calmodulin (cam), and the RNA polymerase second largest subunit (rpb2) genes.
Note: Morphologically, F. melonis was similar to F. pernambucanum. However, the colony diameter of F. melonis on PDA at 25 • C (32.5-38.0 mm in diameter) was clearly smaller than F. pernambucanum (52.5-84 mm in diameter) [47]. The multi-gene phylogenetic analyses indicated that F. melonis clearly distinguished it from the other previously known F. incarnatum-equiseti species complexes and formed a sister clade to F. pernambucanum. However, a pairwise nucleotide comparison of tef-1 data also indicated that F. melonis differed from F. pernambucanum by 2.2% (15/690 bp). Furthermore, F. pernambucanum was isolated from fruit rot disease of muskmelons (C. melo) grown in China [54], fruit rot disease on melons collected from Brazil [55], leaf blight disease found on plum trees (Prunus salicina) grown in China [56] and insects (Aleurocanthus woglumi and Dactylopius opuntiae) indigenous to Brazil [47].

Pathogenicity Test
Conidia of all fungal isolates were used in this experiment. The initial symptoms were observed on both inoculated watermelon and muskmelon fruits two days after inoculation. Initially, small light-brown to brown spots appeared on the fruits. The lesions then enlarged rapidly and developed into brown to dark brown spots on the watermelon fruit and green bruised spots on the melon fruit, both of which were covered with white mycelia surrounding each lesion. After one week of incubation, the lesion diameters on the inoculated fruits were within the ranges of 6.0-6.5 and 2.5-3.0 cm on the watermelons (Figure 5b,c) and muskmelons (Figure 6b,c), respectively. The inoculated watermelons exhibited moderate infections indicated by symptoms of rot, whereas the muskmelons exhibited mild infections. A cross-section revealed that the internal lesion area appeared to be rotting and was surrounded by water-soaked tissue (Figure 5e,f and 6e,f). The diameters of the internal lesions on the watermelon and muskmelon fruits ranged from 6.5-7.5 and 4.0-4.5 cm, respectively. The lesions then spread to the entire fruit and coalesced within 12 and 14 days on the watermelon and muskmelon specimens, respectively, after the occurrence of necrosis. After that, the fruits became completely soft and rotten. These disease symptoms were similar to those seen during the postharvest storage phase. However, no symptoms of plant disease were observed in the inoculation treatments involving sterile distilled water among both wounded watermelon (Figure 5a,d) and muskmelon (Figure 6a,d) fruits. The fungi were re-isolated from symptomatic fruit tissue and then cultured on PDA in order to fulfill Koch's postulates. The re-isolated fungi were identified as F. citrullicola and F. melonis. disease on melons collected from Brazil [55], leaf blight disease found on plum trees (Prunus salicina) grown in China [56] and insects (Aleurocanthus woglumi and Dactylopius opuntiae) indigenous to Brazil [47].

Pathogenicity Test
Conidia of all fungal isolates were used in this experiment. The initial symptoms were observed on both inoculated watermelon and muskmelon fruits two days after inoculation. Initially, small light-brown to brown spots appeared on the fruits. The lesions then enlarged rapidly and developed into brown to dark brown spots on the watermelon fruit and green bruised spots on the melon fruit, both of which were covered with white mycelia surrounding each lesion. After one week of incubation, the lesion diameters on the inoculated fruits were within the ranges of 6.0-6.5 and 2.5-3.0 cm on the watermelons (Figure 5b,c) and muskmelons (Figure 6b,c), respectively. The inoculated watermelons exhibited moderate infections indicated by symptoms of rot, whereas the muskmelons exhibited mild infections. A cross-section revealed that the internal lesion area appeared to be rotting and was surrounded by water-soaked tissue (Figure 5e,f and 6e,f). The diameters of the internal lesions on the watermelon and muskmelon fruits ranged from 6.5-7.5 and 4.0-4.5 cm, respectively. The lesions then spread to the entire fruit and coalesced within 12 and 14 days on the watermelon and muskmelon specimens, respectively, after the occurrence of necrosis. After that, the fruits became completely soft and rotten. These disease symptoms were similar to those seen during the postharvest storage phase. However, no symptoms of plant disease were observed in the inoculation treatments involving sterile distilled water among both wounded watermelon (Figure 5a,d) and muskmelon (Figure 6a,d) fruits. The fungi were re-isolated from symptomatic fruit tissue and then cultured on PDA in order to fulfill Koch's postulates. The re-isolated fungi were identified as F. citrullicola and F. melonis.

Discussion
With regard to the genus Fusarium (Nectriaceae, Hypocreales), currently, there are more than 400 accepted species that have been divided into 23 species complexes [32,57]. The Fusarium species is known to cause several diseases including crown rot, root rot, wilt, fruit rot, leaf spot, stem rot, and seedling blight among cucurbits (cucumbers, muskmelons, pumpkins, squash, and watermelons) worldwide [14,15,23,54,[58][59][60]. Traditionally, Fusarium species are mainly identified by macromorphological characteristics (colony morphology, pigmentation, and type of aerial mycelium), and micromorphological characteristics (the shapes and sizes of conidiophores, conidiogenous cells, macroconidia, microconidia, and the presence or absence of chlamydospores) [30,61,62]. However, morphological characteristics cannot be used to distinguish between the closely related species of Fusarium due to the wide range of morphological variations [30,61]. Therefore, it is essential to identify Fusarium species by applying a molecular approach. Ribosomal DNA [the internal transcribed spacer (ITS) and the large subunit (LSU) regions] and proteincoding [cam, tef-1, β-tubulin (tub2), RNA polymerase largest subunit (rpb1 and rpb2)] genes have provided researchers with a powerful tool in the identification of the Fusarium species [30,31,36,49,[63][64][65]. Nevertheless, using only the ribosomal DNA gene did not resolve the identification of Fusarium at the species level [66,67]. Currently, a combination of morphological characteristics and multi-gene molecular phylogeny are being used for the accurate identification of the Fusarium species [30−32,45,47,36,49,63−65]. In this study, two new Fusarium species, namely F. citrullicola and F. melonis, were obtained from the rot lesions of watermelon and muskmelon fruits, respectively, that were collected from northern Thailand. All fungal species were identified according to their morphological and molecular characteristics in accordance with the identification methods established for the identification approach of Fusarium [30][31][32]47].

Discussion
With regard to the genus Fusarium (Nectriaceae, Hypocreales), currently, there are more than 400 accepted species that have been divided into 23 species complexes [32,57]. The Fusarium species is known to cause several diseases including crown rot, root rot, wilt, fruit rot, leaf spot, stem rot, and seedling blight among cucurbits (cucumbers, muskmelons, pumpkins, squash, and watermelons) worldwide [14,15,23,54,[58][59][60]. Traditionally, Fusarium species are mainly identified by macromorphological characteristics (colony morphology, pigmentation, and type of aerial mycelium), and micromorphological characteristics (the shapes and sizes of conidiophores, conidiogenous cells, macroconidia, microconidia, and the presence or absence of chlamydospores) [30,61,62]. However, morphological characteristics cannot be used to distinguish between the closely related species of Fusarium due to the wide range of morphological variations [30,61]. Therefore, it is essential to identify Fusarium species by applying a molecular approach. Ribosomal DNA [the internal transcribed spacer (ITS) and the large subunit (LSU) regions] and protein-coding [cam, tef-1, β-tubulin (tub2), RNA polymerase largest subunit (rpb1 and rpb2)] genes have provided researchers with a powerful tool in the identification of the Fusarium species [30,31,36,49,[63][64][65]. Nevertheless, using only the ribosomal DNA gene did not resolve the identification of Fusarium at the species level [66,67]. Currently, a combination of morphological characteristics and multi-gene molecular phylogeny are being used for the accurate identification of the Fusarium species [30][31][32]36,45,47,49,[63][64][65]. In this study, two new Fusarium species, namely F. citrullicola and F. melonis, were obtained from the rot lesions of watermelon and muskmelon fruits, respectively, that were collected from northern Thailand. All fungal species were identified according to their morphological and molecular characteristics in accordance with the identification methods established for the identification approach of Fusarium [30][31][32]47].
Both of our new fungal species belong to the F. incarnatum-equiseti species complex (FIESC). FIESC is a highly diverse group that is widely distributed. Accordingly, the majority of them are saprobes that are recognized as pathogens of plants, humans, and animals, and have been found in various environmental habitats [4,[17][18][19]31,46,68,69]. Generally, identification based only on the morphological characteristics of FIESC is difficult because many species have similar outward appearances and display overlapping microscopic characteristics [61,70]. Therefore, molecular multi-gene phylogenetic analysis would be essential to accurately identify the FIESC species [31,45]. Prior to this study, this species complex comprised of 38 recognized phylogenetic species and has been separated into two main clades including the Equiseti clade (19 species) and the Incarnatum clade (19 species) [31,45,47,65,70]. Our multi-gene phylogenetic analyses revealed that two new species, F. citrullicola and F. melonis, formed distinct lineages from previously known species within the Incarnatum clade of FIESC. The different colony characteristics between the two new species indicate that F. citrullicola could more effectively grow on PDA and SNA when compared with F. melonis. On the OA medium, F. citrullicola generated an umbilicate colony, but F. melonis established a flat colony. Micromorphological characteristics indicate that F. citrullicola presents falcate conidia with tapering towards both ends, while F. melonis presents ellipsoidal to falcate conidia that are slightly curved. Additionally, F. citrullicola presents four septate chlamydospores, whereas F. melonis presents two septate chlamydospores. Phylogenetic analyses confirmed that F. citrullicola and F. melonis are actually different species. In addition, F. melonis formed a sister clade to F. pernambucanum. However, a nucleotide comparison of the tef-1 gene showed that F. melonis differed from F. pernambucanum by 2.2% (15/690 bp). Jeewon and Hyde [71] suggested that the nucleotide comparisons of reliable genes should be more than 1.5% different in order to justify the existence of a novel species. Therefore, F. melonis and F. pernambucanum can be considered different species.
To fulfill Koch's postulates, pathogenicity tests were conducted on all isolates of F. citrullicola and F. melonis that had manifested the same symptoms as those observed during the postharvest storage phase. Therefore, F. citrullicola and F. melonis can be considered causal agents for watermelon and muskmelon fruit rot, respectively. Our results are supported by the findings of several previous studies, which indicated that Fusarium is an economically significant plant pathogen. Accordingly, some species of FIESC have been reported to cause fruit rot disease in various cucurbit plants worldwide [4,18]. For example, F. equiseti caused fruit rot disease on watermelon specimens collected in China [72], Malaysia [18], and the United States [24]. Ezrari et al. [16] found that F. equiseti caused pre-and postharvest fruit rot on zucchini plants (Cucurbita pepo) in Morocco. Notably, F. equiseti has been reported as a causal agent of postharvest fruit rot on both oriental melons and cantaloupes grown in Korea [73] and Thailand [4], respectively. Fusarium incarnatum was found as a causal agent of fruit rot on cucumbers grown in Mexico [17] and muskmelons cultivated in Thailand [19]. Fusarium pernambucanum caused fruit rot disease on muskmelons grown in China [54]. In Brazil, F. pernambucanum and F. sulawesiense were found to cause fruit rot on melons [55]. Additionally, other Fusarium species in the F. chlamydosporum species complex (F. chlamydosporum), the F. fujikuroi species complex (F. annulatum, F. moniliforme, F. proliferatum, and F. verticillioides), the F. solani species complex (F. falciforme, F. petroliphilum, and F. solani), the F. oxysporum species complex (F. kalimantanense), the F. sambucinum species complex (F. asiaticum, F. culmorum, F. graminearum, and F. sambucinum) and the F. tricinctum species complex (F. acuminatum) were also found to cause fruit rot on numerous cucurbits (cucumbers, melons, pumpkins, squashes, and watermelons) [22,25,55,[73][74][75][76][77][78][79].
In Thailand, F. equiseti and F. incarnatum were found to be causal agents of rot among cantaloupes [4] and muskmelons [19], respectively. In addition, F. equiseti has been reported as a causal agent of muskmelon wilt disease [80]. In the current study, the disease symptoms observed in incidences of watermelon and muskmelon fruit rot caused by F. citrullicola and F. melonis, respectively, are similar to those that were caused by those known pathogens. Therefore, we have proposed that F. citrullicola and F. melonis be added as the causal agents of fruit rot on watermelons and muskmelons, respectively. However, there have been no prior reports of fruit rot disease on watermelons grown in Thailand. Thus, this study was determined to be the first investigative report on watermelon fruit rot in Thailand. Generally, watermelons and muskmelons are cultivated and harvested in Thailand throughout the cool to early wet seasons (November to June). Thus, during these seasons, fruit rot in watermelons and muskmelons can be found. Follow-up studies are needed to clarify the timing of the infections that occur in these fruits via fungal pathogens. This can be accomplished by monitoring the presence of the disease causal agents in these fruits at different stages of development in cultivation areas during both the pre-and postharvest processes, as well as during the postharvest storage period. Additional investigations will also be necessary to determine the disease's inoculum source and the meteorological conditions that influence infection and disease development.

Conclusions
Fruit rot disease caused by the Fusarium species is one of the most important postharvest diseases of cucurbits in the world. In this study, two new pathogenic Fusarium species, namely F. citrullicola and F. melonis, were isolated from infected watermelon and melon fruits, respectively. Their identification was based on morphological characteristics and multi-gene phylogenetic analyses. The pathogenicity test of F. citrullicola and F. melonis revealed the same symptoms under artificial inoculation conditions as those observed during the postharvest storage phase. Thus, F. citrullicola and F. melonis have been proposed as new causal agents of watermelon and muskmelon fruit rot, respectively. Consequently, further studies involving the distribution of these diseases in other regions of Thailand, and the control of these diseases, will need to be conducted. In order to address the significant economic losses caused by this disease, it will be essential to develop effective monitoring and preventative strategies in the future.