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Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis

Department of Chemical and Biological Sciences, Institute of Biosciences, São Paulo State University (UNESP), Botucatu 18618-689, Brazil
Department of Pathology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand
Faculty of Veterinary Medicine and Animal Science (FMVZ), São Paulo State University (UNESP), Botucatu 18618-681, Brazil
Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo (USP), Bauru 17012-901, Brazil
Department of Microbiology, Immunology and Parasitology, Cell Biology Division, Federal University of São Paulo (UNIFESP), São Paulo 04023-062, Brazil
Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (UNESP), Botucatu 18610-307, Brazil
Graduate Program in Tropical Diseases, Botucatu Medical School (FMB), São Paulo State University (UNESP), Botucatu 18618-687, Brazil
Authors to whom correspondence should be addressed.
Academic Editor: Andrew M. Borman
J. Fungi 2021, 7(6), 423;
Received: 16 February 2021 / Revised: 4 May 2021 / Accepted: 5 May 2021 / Published: 28 May 2021
Pythiosis, whose etiological agent is the oomycete Pythium insidiosum, is a life-threatening disease that occurs mainly in tropical and subtropical countries, affecting several animal species. It is frequently found in horses in Brazil and humans in Thailand. The disease is difficult to diagnose because the pathogen’s hyphae are often misdiagnosed as mucoromycete fungi in histological sections. Additionally, there is no specific antigen to use for rapid diagnosis, the availability of which could improve the prognosis in different animal species. In this scenario, we investigated which P. insidiosum antigens are recognized by circulating antibodies in horses and humans with pythiosis from Brazil and Thailand, respectively, using 2D immunoblotting followed by mass spectrometry for the identification of antigens. We identified 23 protein spots, 14 recognized by pooled serum from horses and humans. Seven antigens were commonly recognized by both species, such as the heat-shock cognate 70 KDa protein, the heat-shock 70 KDa protein, glucan 1,3-beta-glucosidase, fructose-bisphosphate aldolase, serine/threonine-protein phosphatase, aconitate hydratase, and 14-3-3 protein epsilon. These results demonstrate that there are common antigens recognized by the immune responses of horses and humans, and these antigens may be studied as biomarkers for improving diagnosis and treatment. View Full-Text
Keywords: Pythium insidiosum; pythiosis; antigens; diagnosis; therapy; immunoproteomics Pythium insidiosum; pythiosis; antigens; diagnosis; therapy; immunoproteomics
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MDPI and ACS Style

Chechi, J.L.; Rotchanapreeda, T.; da Paz, G.S.; Prado, A.C.; Oliveira, A.L.; Vieira, J.C.S.; Buzalaf, M.A.R.; Rodrigues, A.M.; Santos, L.D.d.; Krajaejun, T.; Bosco, S.d.M.G. Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis. J. Fungi 2021, 7, 423.

AMA Style

Chechi JL, Rotchanapreeda T, da Paz GS, Prado AC, Oliveira AL, Vieira JCS, Buzalaf MAR, Rodrigues AM, Santos LDd, Krajaejun T, Bosco SdMG. Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis. Journal of Fungi. 2021; 7(6):423.

Chicago/Turabian Style

Chechi, Jéssica L., Tiwa Rotchanapreeda, Giselle S. da Paz, Ana C. Prado, Alana L. Oliveira, José C.S. Vieira, Marília A.R. Buzalaf, Anderson M. Rodrigues, Lucilene D.d. Santos, Theerapong Krajaejun, and Sandra d.M.G. Bosco 2021. "Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis" Journal of Fungi 7, no. 6: 423.

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