Light Irradiation Coupled with Exogenous Metal Ions to Enhance Exopolysaccharide Synthesis from Agaricus sinodeliciosus ZJU-TP-08 in Liquid Fermentation

To promote Agaricus sinodeliciosus var. Chaidam ZJU-TP-08 growth and metabolites accumulation, a novel integrated strategy was developed by adopting high levels of metal ions coupled with light treatment. The results revealed that yellow and blue light could significantly promote biomass and exopolysaccharides production, respectively. Furthermore, the yellow–blue light shift strategy could stimulate exopolysaccharides formation. Ca2+ ions coupled with blue light mostly promoted exopolysaccharides production related to oxidative stress, which was 42.00% and 58.26% higher than that of Ca2+ ions coupled with the non-light and dark cultivation without Ca2+ ions in 5-L bioreactor. RNA-seq was performed to uncover the underlined molecular mechanism regulated by light-induced gene expressions in exopolysaccharides biosynthesis and oxidative stress. The findings of this work provide valuable insights into adopting metal ions coupled with the light-assisted method for the macrofungus submerged fermentation for exopolysaccharides production.


Introduction
Macrofungi growth and secondary metabolites production are affected by many factors, including temperature, pH, and growth media [1]. Some metal ions are essential to many physiological processes, such as Ca 2+ and Mg 2+ [2,3], which could affect fungal morphology and cell metabolism. However, in excess, all metals are deleterious to the cells because of an unbalanced oxidative state, mainly by the formation of reactive oxygen species (ROS) [4]. EPS production was a protective response to stress to survive and grow in the metal-contaminated environment [5]. Upon Pantoea agglomerans, Ca 2+ stress caused a decrease in total protein content and an increase in total carbohydrate with a boost in EPS formation [5]. Thus, the performance of P. agglomerans under metal stress indicated a potential candidate for metal bioremediation. Generally, EPS can incorporate nearby metal cations such as Mg 2+ , Fe 3+ , Mn 2+ , and Ca 2+ , which was associated with their viscous and negatively charged characteristics [6]. Additionally, EPS produced from microorganisms could bind heavy metals and allow growth even at high metal concentrations, and thus EPS was used as a biosorbent for heavy metal removal. Heavy metal pollution is a significant problem that has become one of the most severe environmental and human health hazards. It was reported that the increased EPS production in Cyanobacteria. was associated with Cd(II) resistance [7]. These polysaccharides are believed to produce biofilms and protect bacterial cells from desiccation, metallic trace elements, or other environmental stresses, including host immune responses, thus enhancing the chances of colonizing a special ecological niche [8]. Thus, metal ions could potentially act as stressful culture conditions to induce enhanced production of EPS.
Light is an important signal for adaptation to daily environmental changes in nature. During decades of studies, physiological and developmental responses to light irradiation

Microorganisms, Culture Media and Cultivation Conditions
The inclining strain ZJU-TP-08 of A. sinodeliciosus var. Chaidam preserved in the China Center for Type Culture Collection (CCTCC M 2021511) was grown in potato dextrose agar (PDA) solid slant medium, incubated at 18 • C, and maintained at 4 • C in the darkness. Then, the liquid fermentation cultures were grown in 250 mL shaker flasks containing 100 mL culture medium composed of the following ingredients (g/L): glucose 30, peptone 5, KH 2 PO 4 1.0, MgSO 4 ·7H 2 O 0.5, and Vitamin B1 0.5. The screening fermentation was carried out in an Erlenmeyer flask with 10% inoculum on a rotary shaker at 130 rpm and 23 • C for 8 d. After screening for different metal ions and lights, the fermentation control of light shift was conducted in a 5-L bioreactor with specific light irradiation equipment on the outer surface of the bioreactor.

Analytical Methods of Mycelial Biomass and EPS
After fermentation, mycelia were filtered and washed three times with distilled water to remove medium components before freeze-drying to a constant weight. The mycelium biomass (BIO) was determined as the index of mycelial growth. After filtering, 5 mL fermentation broth was added to 95% (v/v) ethanol and shaken well for 24 h at 4 • C before centrifugation. Ethanol was rinsed and freeze-dried. The content of EPS was assayed by the phenol-sulfuric acid method, as previously reported [22]. The calibration curve of EPS was generated by linear regression of OD 490 value against glucose concentrations [23].

Detection of Intracellular Reactive Oxygen Species (ROS)
The intracellular ROS detection was referred to Eruslanov [24] with minor modifications. In brief, after the fermentation broth was treated with light, 2 mL fermentation broth was centrifuged. Then, the precipitate was washed with 1 × PBS three times. Finally, 0.5 mL 1 × PBS was added to obtain the mycelia collection solution. An amount of 1 µL of 10 mM fluorescent probe DCFH-DA (Beyotime Biotechnology Co., Ltd., Haimen, China) was added and incubated in the darkness for 0.5 h at 30 • C. Mycelia were washed with PBS three times. Mycelial ROS was detected by laser scanning confocal microscope at the excitation wavelength of 488 nm and emission wavelength of 525 nm. Additionally, ultrasonic crushing was performed on the recovered pellets at 4 • C. The ultrasonic crushing power was 500 W. Every ultrasonic crushing 2 s was rest for 2 s, the total ultrasonic crushing time was 5 min, then centrifugation, the upper suspension was extracted, and the upper suspension was determined by fluorescence spectrophotometer intensity of DCF in the culture liquid to quantitatively detect the content of mycelial ROS (excitation wavelength 485 nm, emission wavelength 525 nm).

Measurement of Superoxide Anion (O 2− ) and Hydrogen Peroxide (H 2 O 2 )
The detection methods referred to Wu [25] and were minor modified. The mycelia of A. sinodeliciosus were filtered and cleaned with water three times and then filtrated. After removing the mycelia water with absorbent paper, 0.2 g of wet mycelia were weighed and added into 1.5 mL PBS (pH 7.4). Then the supernatant was centrifuged, and the content of O 2 − and H 2 O 2 was detected according to assay kits (Nanjing Jiancheng, Nanjing, China).

Antioxidant Enzymes and Malonaldehyde (MDA) Assay
The cultured mycelia were separated from the fermentation broth according to the above methods, and the cells were broken at 4 • C after adding 1.5 mL PBS. The supernatant obtained after centrifugation was regraded as a crude enzyme solution. Superoxide dismutase (SOD), catalase (CAT), and a glutathione peroxidase (GPx) detection kit (Nanjing jiancheng Co., Ltd., Nanjing, China) were used to detect SOD, CAT, and GPx activity. The protein concentration was measured by the protein detection kit (Sangon Co., Ltd., Shanghai China). An MDA test was also performed in strict accordance with kit instructions (Nanjing Jiancheng Co., Ltd., Nanjing, China).

Transcriptomic Analysis
Total RNA was extracted using Total RNA Miniprep Kit (Axygen, Hangzhou, China) and evaluated for integrity and quality using an Agilent 2100 Bioanalyzer (Agilent Technologies Inc., Amstelveen, The Netherlands)). RNA quantification was determined using the ND-2000 spectrophotometer (Thermo Inc., Wilmington, DE, USA). To analyze the transcriptome, RNA-Seq libraries were prepared from cDNA by Majorbio (Shanghai, China) and sequenced on an Illumina Hiseq4000 (Illumina Inc., San Diego, CA, USA). The raw sequencing reads were screened with the SEQPREP software and SICKLE software to cut out low-quality or default reads. All gained reads were assembled to contigs and singletons. The ORF prediction was carried out using the novel method TRINITY. Differentially expressed genes (DEGs) were identified by DEseq2 (padjust < 0.05 and difference multiple |FoldChange| > 2), as described earlier [26]. We defined gene function analysis and genome annotation from the assembled ORFs using gene ontology (GO) and the Kyoto encyclopedia of genes and genomes (KEGG). The raw RNAseq data in this study were deposited in NCBI under accession number PRJNA729816 (https://www.ncbi.nlm.nih.gov/sra/PRJNA729816 (accessed on 17 May 2020)).

Statistical Analysis
All data were expressed as the mean ± standard deviation (SD). The experiments were performed in triplicates. Fisher's least significance difference (LSD) test was performed using IBM SPSS Statistic 9 (IBM Corp., Armonk, NY, USA), p < 0.05 was considered as the significant threshold in this work.

Effect of LED Irradiation Combined with Different Metal Ions on Mycelial Growth and EPS Production
In order to detect whether the underground mushroom A. sinodeliciosus ZJU-TP-08 is sensitive to light irradiation and metal ions, light-emitting diodes (LEDs) with varying light wavelengths, including red, blue, yellow, purple, green, and white light, were used in the mushroom liquid fermentation. Additionally, different concentrations of calcium ion (Ca 2+ ) and magnesium ion (Mg 2+ ) were also added. As shown in Figure 1, by and large, LED light and metal ion stimulation play an essential role in A. sinodeliciosus growth. In terms of the effect of light on A. sinodeliciosus growth, yellow light had the most significant effect on mycelial biomass (BIO), which was 14.13% higher than that of the continuous darkness group followed by blue and purple light. With regards to EPS production, blue light was found to be optimal. Furthermore, 2 g/L CaCl 2 coupled with blue light fermentation significantly improved EPS production, which was 42% higher than 2 g/L CaCl 2 plus the darkness culture and 58.26% higher than non-metal ion combined darkness fermentation, respectively. In contrast, Mg 2+ ion has an insignificant promoting effect. Hence, Ca 2+ ions play beneficial roles in A. sinodeliciosus fermentation for EPS production.

Yellow-Blue LED Light Shift Cultivation Strategy
In order to further optimize the effect of light modulation on EPS production and mycelial growth by light irradiation, the yellow-blue LED light shift strategy was carried out in A. sinodeliciosus cultivation. The result of dynamically detecting the changes in the A. sinodeliciosus growth curve is shown in Figure 2A. In the culture stage from 0 to 11 d,

Yellow-Blue LED Light Shift Cultivation Strategy
In order to further optimize the effect of light modulation on EPS production and mycelial growth by light irradiation, the yellow-blue LED light shift strategy was carried out in A. sinodeliciosus cultivation. The result of dynamically detecting the changes in the A. sinodeliciosus growth curve is shown in Figure 2A. In the culture stage from 0 to 11 d, the mycelia BIO and EPS synthesis curve both showed a trend of first rising and then flattening. Mycelia growth stabilized on the 5th day. However, the EPS continued to be synthesized and reached a maximum on day 7. To detect whether the photoperiod culture would further affect mycelia growth and EPS synthesis, we changed yellow light into blue light and cultivated A. sinodeliciosus for another 2 d after the 5th day of LEDirradiated fermentation culture. Figure 2B, 2C shows that EPS and BIO accumulation were affected by changing light wavelength during the growth stage. Our findings presented that the yellow-blue LED light shift strategy had no effect on mycelium biomass; however, it could significantly promote the synthesis of EPS. Moreover, adding 2 g/L Ca 2+ into A. sinodeliciosus fermentation with yellow-blue LED light shift strategy had significant overproduced EPS yield increased by 25.48% and 59.05%, compared with yellow-light irradiation (Ca-Y) and the control (CK-D), respectively.

Effect of Different Cultivation Strategies on ROS, H2O2 and O2 − Contents
In order to uncover the underlined mechanism of Ca 2+ ions and LED-light on A. sinodeliciosus growth and EPS production, we detected that the produced mycelial ROS in the different group, as shown in Figure 3A,B, including 2 g/L Ca 2+ and no-mental ion (CK) coupled with darkness (Ca-D or CK-D), yellow (Ca-Y or CK-Y), yellow-blue shift (Ca-YB or CK-YB), by fluorescent probe DCFH-DA via laser confocal microscope, and fluorescence spectrophotometer. The results in Figure 3A revealed that in Ca-YB, Ca-Y, Ca-D, Ck-YB, and Ck-Y groups, the ROS fluorescence intensity was significantly detected. Additionally, the ROS in CK-D was comparatively low. Figure 3B showed the quantitative evaluation of mycelial ROS intensity in foresaid groups. In general, the ROS were Among them, CK-Y, CK-D, and CK-YB represented the no mental ion-treated group with yellow, dark, and yellow-blue shift light, respectively. Ca-Y, Ca-D, and Ca-YB represented the Ca 2+ -treated group with yellow, dark, and yellow-blue shift light, respectively.

Effect of Different Cultivation Strategies on ROS, H 2 O 2 and O 2 − Contents
In order to uncover the underlined mechanism of Ca 2+ ions and LED-light on A. sinodeliciosus growth and EPS production, we detected that the produced mycelial ROS in the different group, as shown in Figure 3A,B, including 2 g/L Ca 2+ and no-mental ion (CK) coupled with darkness (Ca-D or CK-D), yellow (Ca-Y or CK-Y), yellow-blue shift (Ca-YB or CK-YB), by fluorescent probe DCFH-DA via laser confocal microscope, and fluorescence spectrophotometer. The results in Figure 3A revealed that in Ca-YB, Ca-Y, Ca-D, Ck-YB, and Ck-Y groups, the ROS fluorescence intensity was significantly detected. Additionally, the ROS in CK-D was comparatively low. Figure 3B showed the quantitative evaluation of mycelial ROS intensity in foresaid groups. In general, the ROS were produced in all groups, which might contribute to the produced oxidative stress by agitation [27]. Similarly, the ROS intensity in 2 g/L Ca 2+ groups exposed with different LED lights, namely Ca-Y, Ca-D, and Ca-YB, had relatively higher ROS fluorescence intensity. Therefore, compared to ROS in CK-D, CK-Y, and CK-YB groups, it can be concluded that exposing LED-light in A. sinodeliciosus fermentation can stimulate mycelial ROS production. Accordingly, it can be concluded that LED-light exposure will promote ROS production. Besides, adding Ca 2+ into A. sinodeliciosus fermentation also could increase ROS formation. As shown in Figure 3C,D, compared with the Ca-Y group, the transmission of yellow light to blue light during the culture process could significantly improve H 2 O 2 and O 2 − , aggravating the oxidative stresses.

Detection of Glutathione Peroxidase (GPx), Superoxide Dismutase (SOD) and Catalase (CAT) Activity and Lipid Oxidation of Malondialdehyde (MDA)
To further investigate the effect of metal ions and light treatment on A. sinodeliciosus oxidative stress, the activities of SOD, CAT, GPXs, and MDA were also examined. Data from Figure 4 showed that compared with the Ca-Y group, the activities of CAT, SOD, and GPx in the culture process could be significantly increased by converting yellow-blue shift light, which could be related to the content of ROS. While light irradiation and 2 g/L Ca 2+ could increase MDA content to different degrees; thus, oxidative stress was possibly produced.

Differentially Expressed Genes under Ca 2+ and LED-Light Exposure
In order to identify genes that displayed significant changes in expression during Ca 2+ and LED-light exposure, RNA-seq was used to investigate A. sinodeliciosus in response to Ca 2+ and LED-light. After quality evaluation and trimming, over 51.07 million trimmed reads and 7.59 billion bases on average were generated from each sample under darkness conditions and LED-light conditions. All Q30 percentages for the sequences for each library were over 95%. Additionally, over 85.37% of sequenced data was mapped to assemble transcripts from the NCBI database shown in Table 1. In summary, our sequencing data were of high quality and displayed good correlations among biological replicates and thus could be used for subsequent analyses.
Accordingly, a total of 13,894 genes of A. sinodeliciosus were used for aligning the Illumina raw reads. Among them, the tested A. sinodeliciosus groups have a total of 7421 shared genes, as well as a different number of unique genes (data are shown in Supplementary Figure S1), suggesting that these genes were closely associated with A. sinodeliciosus growth and metabolism induced by different light qualities. We found a high number of unigenes with differential expression in treated samples. Differentially expressed genes (DEGs) with a p value-adjusted < 0.05 cut-off were analyzed by comparing the treated and control libraries. As can be seen from Figure 5, the resulting gene expression profiles of the control and treated samples were highly divergent. We discovered 197, 2436, and 2924 genes when comparing Ca-D versus Ca-YB, Ca-Y, and Ca-B, respectively. A total of 57 genes and 140 genes were significantly up-regulated and down-regulated in response to yellow-blue shift strategy. Moreover, after yellow light stimulation, 1128 genes and 1308 genes were significantly up-regulated and downregulated in A. sinodeliciosus mycelia. Additionally, blue light also had a profound effect on DEGs with 1342 up-regulated genes and 1582 down-regulated genes. Interestingly, Ca 2+ also affected gene expressions, which were 253 up-regulated genes and 232 down-regulated genes.

Detection of Glutathione Peroxidase (GPx), Superoxide Dismutase (SOD) and Catalase (CAT) Activity and Lipid Oxidation of Malondialdehyde (MDA)
To further investigate the effect of metal ions and light treatment on A. sinodeliciosus oxidative stress, the activities of SOD, CAT, GPXs, and MDA were also examined. Data from Figure 4 showed that compared with the Ca-Y group, the activities of CAT, SOD, and GPx in the culture process could be significantly increased by converting yellow-blue shift light, which could be related to the content of ROS. While light irradiation and 2 g/L Ca 2+ could increase MDA content to different degrees; thus, oxidative stress was possibly produced.

Differentially Expressed Genes under Ca 2+ and LED-Light Exposure
In order to identify genes that displayed significant changes in expression during Ca 2+ and LED-light exposure, RNA-seq was used to investigate A. sinodeliciosus in response to Ca 2+ and LED-light. After quality evaluation and trimming, over 51.07 million trimmed reads and 7.59 billion bases on average were generated from each sample under darkness conditions and LED-light conditions. All Q30 percentages for the sequences for each library were over 95%. Additionally, over 85.37% of sequenced data was mapped to assemble transcripts from the NCBI database shown in Table 1. In summary, our sequencing data were of high quality and displayed good correlations among biological replicates and thus could be used for subsequent analyses.
Accordingly, a total of 13,894 genes of A. sinodeliciosus were used for aligning the Illumina raw reads. Among them, the tested A. sinodeliciosus groups have a total of 7421 shared genes, as well as a different number of unique genes (data are shown in Supplementary Figure S1), suggesting that these genes were closely associated with A. sinodeliciosus growth and metabolism induced by different light qualities. We found a high number of unigenes with differential expression in treated samples. Differentially expressed genes (DEGs) with a p value-adjusted < 0.05 cut-off were analyzed by comparing the treated and control libraries. As can be seen from Figure 5, the resulting gene expression profiles of the control and treated samples were highly divergent. We discovered 197, 2436, and 2924 genes when comparing Ca-D versus Ca-YB, Ca-Y, and Ca-B, respectively. A total of 57 genes and 140 genes were significantly up-regulated and down-regulated in response to yellow-blue shift strategy. Moreover, after yellow light stimulation, 1128 genes and 1308 genes were significantly up-regulated and downregulated in A. sinodeliciosus mycelia. Additionally, blue light also had a profound effect on DEGs with 1342 up-regulated genes and 1582 down-regulated genes. Interestingly, Ca 2+  also affected gene expressions, which were 253 up-regulated genes and 232 down-regulated genes.

Functional Annotation of Differentially Expressed Genes upon LED-Light Stress
Given that the light-controlled coupling Ca 2+ fermentation has an important effect on the liquid fermentation of A. sinodeliciosus, the identified genes were annotated by GO and KEGG databases to investigate the function of up-regulated genes better. For GO enrichment analysis, the enriched annotations were grouped into three functional categories: biological processes, cellular components, and molecular functions ( Figure S2). Our analysis revealed significantly enriched biological process terms (p < 0.05) associated with cell growth and development, such as positive regulation of metabolic process (GO:

Functional Annotation of Differentially Expressed Genes upon LED-Light Stress
Given that the light-controlled coupling Ca 2+ fermentation has an important effect on the liquid fermentation of A. sinodeliciosus, the identified genes were annotated by GO and KEGG databases to investigate the function of up-regulated genes better. For GO enrichment analysis, the enriched annotations were grouped into three functional categories: biological processes, cellular components, and molecular functions ( Figure S2). Our analysis revealed significantly enriched biological process terms (p < 0.05) associated with cell growth and development, such as positive regulation of metabolic process (GO: 0008152), biological regulation (GO:0065007), cellular process (GO:0009987), and response to stimulus (GO:0050896). Among them, compared with yellow light, blue light enhanced the expression of genes related to response to stimuli. Within the cellular component category, membrane part (GO:0044425), cell part (GO:0044464), and organelle (GO:0043226) were rich in DEGs. For further detecting the DEGs in A. sinodeliciosus response to light stimulation, GO enrichment analysis showed in Figure 6A revealed that the molecular function, 'transmembrane transporter activity' (GO:00022857, 378 genes), 'transporter activity' (GO:0005215, 381 genes), 'oxidoreductase activity' (GO:0016491, 501 genes), 'iron ion binding' (GO:0005506, 82 genes), 'FAD binding' (GO:0071949, 42genes), 'catalytic activity' (GO:0003824, 14971 genes) were abundantly enriched under blue, yellow, and yellow-shift light conditions, along with integral or intrinsic component of the membrane and cell surface receptor signaling pathway. KEGG analysis was used to classify the functions of annotated DEGs further. In addition to the pathways related to metabolisms, such as tyrosine metabolism, glycine, serine, and threonine metabolism, Figure 6B revealed that mitogenactivated protein kinase (MAPK) signaling pathways and oxidative phosphorylation were enriched in DEGs.
'catalytic activity' (GO:0003824, 14971 genes) were abundantly enriched under blue, yellow, and yellow-shift light conditions, along with integral or intrinsic component of the membrane and cell surface receptor signaling pathway. KEGG analysis was used to classify the functions of annotated DEGs further. In addition to the pathways related to metabolisms, such as tyrosine metabolism, glycine, serine, and threonine metabolism, Figure 6B revealed that mitogen-activated protein kinase (MAPK) signaling pathways and oxidative phosphorylation were enriched in DEGs.  In order to further investigate the effects of yellow (Y) and blue light (B) on liquid fermentation of A. sinodeliciosus, we analyzed DEGs by GO and KEGG enrichment analysis at the transcriptome level. The GO enrichment analysis shown in Figure 7A,B revealed that the DEGs of A. sinodeliciosus under blue-light and yellow-light stimulation were significantly enriched in membrane component and part, oxidoreductase activity, monooxygenase activity, and transmembrane transporter activity, which were related to cell composition and molecular function. As shown from the KEGG enrichment analysis in Figure 7C,D, DEGs in A. sinodeliciosus response to blue light and yellow light were mainly concentrated in tyrosine metabolism, MAPK signaling pathway, amino sugar and nucleotide sugar metabolism, and starch and sucrose metabolism. Additionally, compared with yellow light, DEGs of the peroxisome metabolism pathway were significantly enriched in blue-light stimulation. monooxygenase activity, and transmembrane transporter activity, which were related to cell composition and molecular function. As shown from the KEGG enrichment analysis in Figure 7C,D, DEGs in A. sinodeliciosus response to blue light and yellow light were mainly concentrated in tyrosine metabolism, MAPK signaling pathway, amino sugar and nucleotide sugar metabolism, and starch and sucrose metabolism. Additionally, compared with yellow light, DEGs of the peroxisome metabolism pathway were significantly enriched in blue-light stimulation. Therefore, in order to elucidate the polysaccharide biosynthesis pathway of A. sinodeliciosus stimulated by blue light, we detected the up-regulated genes and gene clusters involved in the saccharide unit. The result presented in Supplementary materials Table S1 revealed that blue light could up-regulate genes related to fructose and mannose metabolism, galactose metabolism, and starch and sucrose metabolism. In this work, Table 2 revealed that blue-light stimulation led to a significant activation of the transcription of genes involved in DNA repair and cellular response to DNA damage stimulus. In addition to the above, consistent with the previous results, blue light up-regulated DEGs in peroxidase activity, catalase activity, peroxiredoxin activity, and hydrogen peroxide catabolic process to respond to oxidative stress. Hence, the transcriptomics results re- Therefore, in order to elucidate the polysaccharide biosynthesis pathway of A. sinodeliciosus stimulated by blue light, we detected the up-regulated genes and gene clusters involved in the saccharide unit. The result presented in Supplementary materials Table S1 revealed that blue light could up-regulate genes related to fructose and mannose metabolism, galactose metabolism, and starch and sucrose metabolism. In this work, Table 2 revealed that blue-light stimulation led to a significant activation of the transcription of genes involved in DNA repair and cellular response to DNA damage stimulus. In addition to the above, consistent with the previous results, blue light up-regulated DEGs in peroxidase activity, catalase activity, peroxiredoxin activity, and hydrogen peroxide catabolic process to respond to oxidative stress. Hence, the transcriptomics results revealed that blue-light stimulation could provoke the antioxidative activity and stimulate EPS production by A. sinodeliciosus. To sum up the findings of this work, the possible mechanism of different LED lights on mycelia growth and EPS production by A. sinodeliciosus was postulated, which is presented in Figure 8. vealed that blue-light stimulation could provoke the antioxidative activity and stimulate EPS production by A. sinodeliciosus. To sum up the findings of this work, the possible mechanism of different LED lights on mycelia growth and EPS production by A. sinodeliciosus was postulated, which is presented in Figure 8.

Discussion
Agaricus sinodeliciosus var. Chaidam, a rare underground mushroom, has not yet been cultivated successfully. The effect of light coupled with metal ions on A. sinodeliciosus growth, as well as its underlying molecular mechanisms and genes, remain relatively unclear. In summary, our findings demonstrated that the addition of light exposure and metal ions could have a great effect on Agaricus sinodeliciosus var. Chaidam growth and EPS production. In our study, 2 g/L CaCl 2 coupled with blue light fermentation significantly improved EPS production, which was 42% higher than 2 g/L CaCl 2 plus the darkness culture and 58.26% higher than non-metal ion combined darkness fermentation, respectively. Previous research [28] demonstrated that LED illumination exhibited an adverse effect on P. eryngii growth but increased EPS production by blue light, which was similar to our research findings. Blue light could also be considered as the best treatment to enhance the production efficiency of oyster mushrooms [29]. Moreover, Kho [30] reported that blue LED contributed to the highest EPS production of Cordyceps militaris, but the red LED was the best for mycelia biomass growth, which was inconsistent with our findings. Therefore, we hypothesized that different mushrooms respond differently to light stimulation. It was reported that adding 10 mM Ca 2+ to static liquid cultures of Ganoderma lucidum proved to be a useful strategy to enhance the production of ganoderic acids (GAs) [2]. Moreover, simultaneously adopting the strategies of multiple Cu 2+ additions coupled with threestage light irradiation could enhance total GAs produced by G. lucidum [31]. Therefore, the culture strategy of supplementing metal ions with light treatment is beneficial to obtain fermentation products of macrofungus. Notably, EPSs are a class of biopolymers that are secreted extracellularly by macrofungi to withstand adverse conditions [32]. Upon exposure to environmental stresses, the mushroom could secrete polysaccharides to cope with the adverse environment. Intriguingly, metal ions can stimulate polysaccharide synthesis. In metallic trace element pollution, bacterial exopolymers have become an alternative of interest as metal-binding agents in the detoxification of contaminated water [33]. It was demonstrated that Ca 2+ could affect Lentinus edodes mycelial growth and polysaccharide biosynthesis [34], which was similar to the present results.
Light, as a stress factor, could induce the generation of ROS to regulate the biosynthesis of fungal metabolites [35]. Moreover, metal-induced toxicity observed in different living organisms was associated with oxidative damage [36]. ROS, including oxygen radicals and some non-radical derivatives of oxygen (O 2 ), including superoxide anion (O 2 − ) and hydrogen peroxide (H 2 O 2 ), is produced as a typical cellular metabolic byproduct in living cells [37]. Therefore, O 2 − and H 2 O 2 production in the mycelia were also measured in Figure 3C,D. Ca 2+ significantly increased the level of oxidative stress.
The effect of metal ions and light treatment on A. sinodeliciosus oxidative stress can also be understood by checking the oxidative system. The activities of SOD, CAT, GPXs and MDA content were also studied. SOD enzymes could control the levels of a variety of ROS, thus both limiting the potential toxicity of these molecules and controlling broad aspects of cellular life that are regulated. SOD enzymes catalyze the dismutation of superoxide (O 2 − ), generating hydrogen peroxide (H 2 O 2 ) [37]. CAT and GPXs, the key antioxidant enzymes produced to decompose H 2 O 2 [38], were shown to be the most significantly induced antioxidant enzymes under oxidative stress in filamentous fungus [39]. Therefore, cellular H 2 O 2 concentration and CAT activity are the appropriate indicators of oxidative stress and the fungal cellular response to it [40].
Polysaccharides are considered the major and most-studied bioactive component in macrofungus. Previous research revealed that A. sinodeliciosus-derived EPS is mainly composed of monosaccharides, such as xylose, arabinose, galactose, mannose, and glucose [41]. In order to investigate the EPS biosynthesis pathway of A. sinodeliciosus, we detected the genes and gene clusters involved in the saccharide unit, such as sucrose, mannose, and galactose biosynthesis by transcriptomics analysis. Our RNA-seq results showed that blue light could up-regulate genes related to fructose, mannose, galactose, and sucrose metabolism, which could be one reason that accounted for the increase in overproduced polysaccharide synthesis [42]. Kojima [15] demonstrated that blue light could enhance the key enzyme related to shikimic acid synthesis in submerged liquid fermentation of oyster mushroom, thus promoting the synthesis of secondary metabolite shikimic acid. Unfortunately, we did not find significant changes in key genes involved in polysaccharide synthesis, including the reported phospho-glucose isomerase (PGI), phosphoglucomutase (PGM), and UDP-glcpyrophosphorylase (UGP) [34]. We hypothesized that the increased EPS synthesis might have other pathways besides enzymes increase. Importantly, DNA was constantly exposed to damaging threats coming from photoinduced oxidative stress [43]. It was noted that blue-light stimulation led to a significant activation of the transcription of genes involved in DNA repair and cellular response to DNA damage stimulus. Threats to DNA stability can be triggered by oxidative stress induced by the presence of metabolic reactive radical and ROS or by photoreactivity, which could have a strong influence on the biological processes governed by DNA, as well as in the indirect activation or transduction of signal cascades, resulting in different outcomes for the life of involved cells [44].

Conclusions
In summary, various wavelengths of LEDs supplemented with metal ions were used to regulate mycelial biomass and EPS production in A. sinodeliciosus cultivation. Yellow light and blue light were the most significant promoter of mycelium and EPS production, respectively. Moreover, the combination of yellow and blue light in different fermentation stages could significantly promote the synthesis of EPS. Finally, the enhanced EPS production was obtained by blue light coupled with Ca 2+ stimulation, which was found to attribute to oxidative stress. Our findings provided a novel cultivation strategy for high-value mushrooms to promote the production of active substances under different stresses.