Isolation and Identification of Alternaria alternata from Potato Plants Affected by Leaf Spot Disease in Korea: Selection of Effective Fungicides

Brown leaf spot disease caused by Alternaria spp. is among the most common diseases of potato crops. Typical brown spot symptoms were observed in commercial potato-cultivation areas of northern Korea from June to August 2020–2021. In total, 68 isolates were collected, and based on sequence analysis of the internal transcribed spacer (ITS) region, the collected isolates were identified as Alternaria spp. (80.9%). Phylogenetic analysis revealed that a majority of these isolates clustered within a clade that included A. alternata. Additionally, the ITS region and rpb2 yielded the most informative sequences for the identification of A. alternata. Pathogenicity tests confirmed that the collected pathogens elicited symptoms identical to those observed in the field. In pathogenicity tests performed on seven commercial cultivars, the pathogens exhibited strong virulence in both wound and non-wound inoculations. Among the cultivars tested, Arirang-1ho, Arirang-2ho, and Golden Ball were resistant to the pathogens. Furthermore, among the fungicides tested in vitro, mancozeb and difenoconazole were found to be effective for inhibiting mycelial growth. In summary, our findings suggest that A. alternata plays a critical role in leaf disease in potato-growing regions and emphasise the necessity of continuous monitoring and management to protect against this disease in Korea.


Introduction
Potatoes (Solanum tuberosum L.) are a staple non-cereal food crop and are the fourthmost-productive crop after maize, wheat, and rice worldwide [1].Various pathogenic infections threaten potato crops and can lead to poor quality and reduced yield.These infections include late blight, caused by Phytophthora infestans; early blight, caused by Alternaria solani; and brown leaf spot disease, caused by Alternaria alternata [2].In a previous study conducted in Korea, more than 60% of potato leaves exhibited brown leaf spots caused by A. alternata infection under conditions of high humidity and warm temperatures that ranged from 12 • C at night to 30 • C during the daytime [3].

Fungal Cultures and DNA Extraction
All the collected isolates were subjected to DNA extraction.Fungal isolates were grown in 5 mL of potato dextrose broth (Difco Laboratories, Detroit, MI, USA) at 25 • C for 7 days.Genomic DNA was isolated as described previously [15] or purified using the NucleoSpin Plant kit (Macherey-Nagel, D űren, Germany) according to the manufacturer's instructions.The DNA concentration was estimated using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Inc. Wilmington, NC, USA).The DNA concentration was adjusted to 12.5 ng/µL for each isolate and subjected to PCR amplification.

PCR and Sequencing
PCR was performed using an ABI 2720 Thermal Cycler (Applied Biosystems, Foster City, CA, USA).PCR amplification was performed with 25 ng of genomic DNA and 2 pmol/L of each primer (Table 1) using the i-StarMAX II PCR master mix (iNtRON Biotechnology Inc., Seongnam, Republic of Korea).The amplification conditions were as follows: (a) initial denaturation at 96 • C for 1 min; (b) 2 cycles of denaturation at 94 • C for 1 min, annealing at 52 • C for 1 min, and elongation at 72 • C for 1 min; (c) 28 cycles of denaturation at 94 • C for 30 s, annealing at 55 • C for 30 s, and elongation at 72 • C for 1 min; and (d) elongation at 72 • C for 3 min.TCGCAGTAAGACACA TTCTACG [24] a ITS: internal transcribed spacer regions 1 and 2 and intervening 5.8S nrDNA, gapdh: glyceraldehyde 3-phosphate dehydrogenase, tef1: translation elongation factor 1-alpha, rpb2: RNA polymerase second largest subunit, Alt a 1: Alternaria major allergen gene, endoPG: endopolygalacturonase, and OPA10-2: anonymous region.
PCR products were resolved via 0.8% agarose gel electrophoresis and bidirectionally sequenced by the Bioneer sequencing service (Bioneer Inc., Daejeon, Republic of Korea) on both strands with the same primers used for PCR amplification.Sequence assembly was performed using the SeqMan program DNAStar (Madison, WI, USA) and CodonCode Aligner V3.5.4.software (CodonCode Co., Centerville, MA, USA).The aligned sequences were subjected to a BLASTn search in the GenBank database "http://www.ncbi.nlm.nih.gov/BLAST (accessed on 1 December 2023)".All of the generated sequences were deposited in GenBank (Supplementary Table S1).

Phylogenetic Analysis
Phylogenetic analysis was performed on A. alternata isolates, and the dominant group was identified using ITS region sequencing.Multiple sequence alignments of the concatenated sequences were generated using ClustalX [24] and manually adjusted.Sequence divergence was estimated using the MEGA computer package version 11 [25] and the Tamura-Nei model of evolution [26].Phylogenetic analyses of the sequence data consisted of a maximum-likelihood analysis of both the individual data partitions and the combined dataset.

Pathogenicity Test
To fulfil Koch's postulates and confirm that these fungi could infect potato leaves, 1month-old potato plants (S. tuberosum cultivar (cv.)Superior) grown in a 25 • C growth chamber were sprayed with a conidial suspension (1 × 10 6 conidia/mL) containing 250 ppm Tween 20 prepared from 7-to-14-day-old cultures of the selected Alternaria spp.isolates.Sterile distilled water was used as the control.The inoculated plants were placed in a plastic box (50 × 40 × 45 cm) to maintain high humidity and incubated in the dark at 25 • C for 1 day.The box was transferred to a growth chamber, and the plants were grown under a 16-h photoperiod with fluorescent lighting and maintained at a temperature of 25 • C and humidity >70%.Disease severity was measured 7 days after inoculation.The assay was performed in triplicate.

Virulence Test on Commercial Cultivars
Seven commercial potato cultivars, Arirang-1ho, Arirang-2ho, Golden Ball, Daekwang, Daeji, Superior, and Chubaek, were obtained from the Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration, Korea.The cultivars were grown in a greenhouse (23-30 • C).For large-scale screening, a detached leaf assay was performed using leaves from 45-day-old plants from all seven potato cultivars.
Healthy leaves were placed in a plastic box and maintained in a watered state using cotton.Then, a single leaf was inflicted with wounds 10 times at each of the three inoculation sites using a hand acupuncture needle (0.18 × 8 mm, Qingdao Dongbang Medical Co., Ltd., Shandong, China).The inoculum was prepared with 6-mm agar plugs from the 7-day-old A. alternata culture in a V8 juice agar medium.For inoculation, the mycelial agar plugs were placed upside down onto the detached leaves.
Inoculation was conducted in the dark at 25 • C. At 1 day post-inoculation (dpi), the inoculum was removed from the infection sites and the containers were incubated in a growth chamber (16 h light with >70% humidity and at 25 • C).The symptoms were observed at 7 dpi.An uninoculated V8 juice-agar plug was used as a control.All the experiments were performed twice.Diseased leaf area (DLA) was calculated by measuring the leaf area with observable symptoms relative to the total observed area using ImageJ software version 1.48 [27].The formula used was as follows: Diseased leaf area (%) = (leaf area with visible symptoms/total observed area) × 100. (1)

In Vitro Screening of Fungicide Sensitivity
The ability of the fungicides to inhibit the radial growth of the A. alternata isolates was assayed.To select a suitable fungicide for the control of A. alternata isolates, different fungicides with varied mechanisms of action were tested: mancozeb and chlorothalonil; difenoconazole; boscalid and pydiflumetofen; kresoxim-methyl; and thiophanate-methyl (Table 2).After the A. alternata isolates were selected, the ability of the fungicides to inhibit the radial growth of these isolates was evaluated using the agar dilution method [28].The selected fungicide was added to the PDA medium before the medium solidified, and the mycelial plug from the edge of the hyphae was cultured for 7 days in the PDA medium using a 6 mm cork borer.After 7 days, the mycelial plugs were inoculated into the fungicide medium.
After 7 days of incubation at 25 • C, the amounts of radial growth in both the control (C, PDA) and treated (T, PDA amended with fungicide) groups were measured.The percentages of radial growth inhibition (I) and corrected inhibition (IC) were calculated as previously described [29].In brief, two formulae were used: where C is the diameter of the fungal colony from the selected isolate on the PDA plate, T is the diameter of the colony on the treated plate, and C 0 is the diameter of the primary fungal mycelial disc (6 mm).

Statistical Analysis
The results of the study are presented as the mean ± standard deviation.Statistical analysis was performed using one-way analysis of variance in IBM SPSS software (Ver.20.0, SPSS Inc., Chicago, IL, USA).Duncan's multiple range test was used to determine significance at the 95% probability level.

Collection of Fungal Isolates from Potato Plants with Brown Leaf Spot Disease
We investigated the occurrence of diseases affecting potatoes cultivated in three northern regions (Yeoncheon, Goseong, and Baengnyeongdo) of Korea from June and July 2020 to 2021 (Table 3).Brown leaf spot disease was observed in 2020 and 2021, with a particularly severe outbreak in Yeoncheon in 2021 (Figure 1a).The molecular identification of the isolates was performed by analysing their morphological characteristics and ITS region sequences, then cross-referencing the obtained ITS sequences with the results of the NCBI BLAST search (Table 3).

Phylogenetic Analysis of Alternaria spp. Isolates Using Seven Barcoding Genes
Given that members of the Alternaria genus were the predominant fungal species detected throughout the study period, we obtained nucleotide sequences for seven barcoding genes: ITS, gapdh, tef1, rpb2, Alt a 1, endoPG, and OPA10-2, for species-level identification of the 55 selected Alternaria spp.isolates.For parts of the analysis, we used individual gene sequences (ITS (Supplementary Figure S1), gapdh (Supplementary Figure S2), tef1 (Supplementary Figure S3), rpb2 (Supplementary Figure S4), Alt a 1 (Supplementary Figure S5), endoPG (Supplementary Figure S6), and OPA10-2 (Supplementary Figure S7)).Additionally, a concatenated multigene phylogeny encompassing all seven genes was generated (Figure 2).The multigene phylogenetic tree revealed that 51 isolates corresponded to A. alternata, while the remaining four isolates comprised two strains of A. arborescens and two strains of A. solani (Figure 2).Notably, the phylogenetic tree generated from rpb2 sequences showed similar clustering (Supplementary Figure S4) to that seen in the concatenated seven-gene phylogenetic tree; two isolates (SYP-F0352 and SYP-F035) clustered with the type strain A. solani CBS109157, and two isolates (SYP-F0713 and SYP-F0714) were associated with A. arborescens CBS 102605 (Supplementary Figure S4).However, in the remaining single-gene tree, isolates of A. alternata, A. arborescens, and A. solani could not be clearly distinguished.F0714) were associated with A. arborescens CBS 102605 (Supplementary Figure S4).However, in the remaining single-gene tree, isolates of A. alternata, A. arborescens, and A. solani could not be clearly distinguished.S1).The tree was constructed using the maximum-likelihood method, and bootstrap values (1000 replications) are shown in front of each node.MEGA version X software was used for the analysis.S1).The tree was constructed using the maximum-likelihood method, and bootstrap values (1000 replications) are shown in front of each node.MEGA version X software was used for the analysis.

Pathogenicity Test
Infections caused by Alternaria spp.involve the direct invasion of host plants through the stomata and/or wounds [30].To investigate the possibility of infection through stomata, we inoculated conidia onto the entire unwounded surface of samples from the Superior potato cultivar.Based on the results of the phylogenetic analysis, the isolates were clustered into three distinct groups, and we selected isolates from each of these the three groups for virulence testing (Figure 2): six isolates (SYP-F0939, SYP-F0942, SYP-F0934, SYP-F0941, SYP-F0944, and SYP-F0946) from Group I, one isolate (SYP-F0936) from Group II, and four isolates (SYP-F0935, SYP-F0940, SYP-F0943, and SYP-F0945) from Group III.
Disease symptoms were first observed 3 days post-inoculation (dpi) in most isolates, and severe necrotic brown spot symptoms were observed at 7 dpi.Necrosis progressed from the outer edges to the inner regions of the leaves, causing them to turn black and wilt (Figure 3).These symptoms are similar to those observed in the field (Figure 1).In particular, two strains classified in Group I, SYP-F0939 and SYP-F0942, caused severe disease, leading to the death of all inoculated potato seedlings.No symptoms were observed in the control seedlings.The isolates retrieved from all diseased leaves were confirmed to be A. alternata based on their sequences at the rpb2 locus, establishing A. alternata as the causative agent of this disease.

Pathogenicity Test
Infections caused by Alternaria spp.involve the direct invasion of host plants through the stomata and/or wounds [30].To investigate the possibility of infection through stomata, we inoculated conidia onto the entire unwounded surface of samples from the Superior potato cultivar.Based on the results of the phylogenetic analysis, the isolates were clustered into three distinct groups, and we selected isolates from each of these the three groups for virulence testing (Figure 2): six isolates (SYP-F0939, SYP-F0942, SYP-F0934, SYP-F0941, SYP-F0944, and SYP-F0946) from Group I, one isolate (SYP-F0936) from Group II, and four isolates (SYP-F0935, SYP-F0940, SYP-F0943, and SYP-F0945) from Group III.
Disease symptoms were first observed 3 days post-inoculation (dpi) in most isolates, and severe necrotic brown spot symptoms were observed at 7 dpi.Necrosis progressed from the outer edges to the inner regions of the leaves, causing them to turn black and wilt (Figure 3).These symptoms are similar to those observed in the field (Figure 1).In particular, two strains classified in Group I, SYP-F0939 and SYP-F0942, caused severe disease, leading to the death of all inoculated potato seedlings.No symptoms were observed in the control seedlings.The isolates retrieved from all diseased leaves were confirmed to be A. alternata based on their sequences at the rpb2 locus, establishing A. alternata as the causative agent of this disease.

Virulence Test on Commercial Cultivars
Typical symptoms of Alternaria spp.infection were observed in both wounded and non-wounded leaves within 7 days of the initial exposure in the detached leaf assay.These symptoms were similar to those observed in the field.The results of the virulence test for all seven cultivars indicated that the disease incidence depended on both the potato cultivar and the A. alternata isolate in wounding and non-wounding inoculations.The control group showed no symptoms on either wounded or non-wounded leaves (Figure 4a).
Typical symptoms of Alternaria spp.infection were observed in both wounded and non-wounded leaves within 7 days of the initial exposure in the detached leaf assay.These symptoms were similar to those observed in the field.The results of the virulence test for all seven cultivars indicated that the disease incidence depended on both the potato cultivar and the A. alternata isolate in wounding and non-wounding inoculations.The control group showed no symptoms on either wounded or non-wounded leaves (Figure 4a).Potato cv.Chubaek: typical progressive or acute symptoms included brownish-black lesions, leaf discoloration, and purple coloration in response to some isolates (Figure 4a).Among the seven cultivars tested, Chubaek was relatively abundant.The DLA rates for all isolates (12, 2, and 12 isolates selected from A. alternata groups I, II, and III, respectively, see Figure 2) were 33.2% and 52.2% with non-wounding (Figure 4b) and wounding (Figure 4c) inoculations, respectively.These results indicate the heightened susceptibility of Chubaek to the A. alternata isolates obtained from the potato fields, compared to the other six cultivars.
The incidence of disease was the lowest in potato cvs.Arirang-1ho, Arirang-2ho, and Golden Ball inoculated with any of the isolates (Figure 4).The DLA rates of cvs.Arirang-1ho, Arirang-2ho, and Golden Ball were 1.2%, 2.2%, and 3.0%, respectively, for non-wounding inoculations (Figure 4a,b).For wounding inoculations (Figure 4c), the values were 11.5%, 12.3%, and 9.1%, respectively, indicating resistance to the disease.Consistent reisolation of the pathogen from symptomatic plants of most cultivars confirmed A. alternata as the causative agent responsible for the observed symptoms.

Selection of Appropriate Fungicides for Potato Brown Spot Disease in Korea
In total, 10 isolates were used for the fungicide-selection experiment, with two selected from each of the three groups (I, II, and III) of A. alternata and two isolates each of A. arborescens and A. solani (Figure 2).The fungicides mancozeb and difenoconazole effectively controlled the mycelial growth of all isolates, with control rates ranging from 100% to 73.1% (Figure 5, Table 4).Pydiflumetofen and boscalid exhibited lower, yet still significant, inhibitory effects on mycelial growth compared to mancozeb and difenoconazole.In contrast, chlorothalonil, kresoxim-methyl, and thiophanate-methyl had limited effectiveness, with control of mycelial growth rates ranging from a maximum of 56% to a minimum of 3.2% (Figure 5; Table 4).

Discussion
This study aimed to identify the causal pathogen responsible for potato brown leaf spot in the three northern regions of Korea from June to August in 2020-2021, identify potato cultivars resistant to this pathogen, and select the most effective commercially available fungicides for controlling this pathogen in Korean potato crops.
Our results strongly suggest that A. alternata is the major pathogen responsible for

Discussion
This study aimed to identify the causal pathogen responsible for potato brown leaf spot in the three northern regions of Korea from June to August in 2020-2021, identify potato cultivars resistant to this pathogen, and select the most effective commercially available fungicides for controlling this pathogen in Korean potato crops.
Our results strongly suggest that A. alternata is the major pathogen responsible for brown leaf spot disease in these regions.Although A. alternata has long been reported as a major pathogen in potato-cultivation areas in Europe [31,32], the United States [6,10,13], China [1], Israel [5], South Africa [33], and Russia [34], it was first reported in Korea only in 2023 [3].Subsequent research on the pathogen's distribution, the presence of resistant varieties, and the selection of effective control agents against A. alternata in Korea is lacking.
The phylogenetic analysis revealed genetic disparities, showing three distinct clusters-groups I, II, and III-among A. alternata isolates.These groups were primarily distinguished based on the ITS region and the rpb2 gene.In addition, the rpb2 gene sequences were found to be highly effective in identifying strains of A. arborescens, A. solani, and A. alternata.Previous studies have highlighted employing a combination of gene sequences in phylogenetic analysis for distinguishing among Alternaria spp.[1,2,10,13,14,32], and our study accurately identified A. alternata using a set of gene sequences that included the ITS region, gapdh, tef1, rpb2, Alt a1, endoPG, and OPA 10-1.We believe that more extensive genetic information can offer deeper insights.However, despite the genotype-based clustering into groups I, II, and III, these differences did not translate into notable differences in phenotype.No significant differences were noted in pathogenicity, virulence across the seven cultivars, or sensitivity to fungicides among these genotype-based groups.This result suggests that these genetic distinctions might not be practically significant.
In the northern region of Korea, the period from late June to mid-July is a critical time for the growth and harvest of potatoes, directly impacting potential yield losses.Due to recent climate change, significant temperature fluctuations between day and night result in wet leaves and prolonged high humidity during the rainy season.These conditions may elevate the risk that potato plants will be susceptible to various pathogens, including A. alternata.Based on our survey, the data indicate that A. alternata is dominant over A. solani or P. infestans in Korea (see Table 3).In a previous study, it was noted that the occurrence of late blight decreases when the sowing date is shifted to a cooler season [35].Therefore, it is expected that using climate-change scenarios and developing a model will enable researchers to identify actions that can be taken to decrease the prevalence of the disease.
The majority of the differences between A. alternata and A. solani or P. infestans may be attributed to chemical treatments.Choi et al. (2023) recently reported a shortage of fungicides developed to control A. alternata, the pathogen responsible for potato leaf spot disease in Korea [3].Therefore, if a farmer sprayed fungicides on the potato field, they most likely controlled the spread of A. solani or P. infestans.Based on this information, the A. alternata isolated in this study is likely resistant to fungicides, especially considering that A. alternata was the predominant isolate.This finding is significant because both A. solani and P. infestans thrive in high humidity and at a wide range of temperatures [35][36][37].On the other hand, it is highly likely that A. alternata is not resistant to mancozeb and chlorothalonil, which were not used in the fields.Therefore, testing for resistance to fungicides should be conducted.Additionally, although this issue was not investigated in our study, the extensive use of QoI fungicides has been reported to lead to resistance in anthracnose on pepper plants in Korea [38,39].
Notably, all 10 selected isolates exhibited resistance to the QoI fungicide, kresoximmethyl (Figure 5; Table 4).Therefore, testing for resistance to other QoI fungicides, such as azoxystrobin, is warranted; the widespread use of QoIs in commercial potato fields in Korea has led to a high probability of resistance development in Alternaria spp.Dube et al. (2014) reported the presence of A. alternata isolates resistant to azoxystrobin, which is commonly used to control early blight [33].These isolates had a single point mutation in the cyt b gene of the mitochondrial genome, resulting in an amino-acid substitution from glycine to alanine at position 143 (G143A) [33].This result highlights the feasibility of confirming resistance to QoI-class pesticides through PCR amplification and sequencing.The finding of resistance emphasises the importance of continuous monitoring and management of resistant strains of Alternaria spp. to implement effective fungicide-application strategies.

Conclusions
This study identified A. alternata, A. arborescens, and A. solani as the causative agents of brown leaf spot disease in potatoes in Korea through phylogenetic analyses.Among these, A. alternata was found to be the major pathogen.Pathogenicity tests confirmed that all the selected isolates resulted in the same symptoms observed in the field.When inoculated with and without wounds, Arirang-1ho, Arirang-2ho, and Golden Ball exhibited resistance; Daekwang, Daeji, and Superior demonstrated moderate resistance; and Chubaek was found to be susceptible.In vitro screening identified mancozeb and difenoconazole as the most effective fungicides for inhibiting fungal growth, making them the most suitable fungicide options.Our study revealed that brown spot disease caused by A. alternata, which has been previously reported in various countries, has the potential to become a major disease affecting potato production in Korea.Further research is required to investigate the genetic diversity of this pathogen and environmental factors influencing the occurrence of this disease.

Figure 1 .
Figure 1.Naturally occurring leaf brown spot on potato plants, representatives of collected isolates, and percentage distribution of fungal isolates during 2020-2021 in Korea.(a) Brown spot symptoms on potato leaves collected from Yeoncheon (June 2021).Symptoms on the front of the leaves (left), back of the leaves (centre), and stem (right); (b) 14-day-old PDA cultures of five representative fungal species, including Alternaria spp.(far left): Fusarium spp.(second from the left), Boeremia spp.(centre), Stagonosporopsis spp.(right of centre), and Colletotrichum sp.(far right); (c) Spores of each fungus.The scale bar indicate 10 m.

Figure 1 .
Figure 1.Naturally occurring leaf brown spot on potato plants, representatives of collected isolates, and percentage distribution of fungal isolates during 2020-2021 in Korea.(a) Brown spot symptoms on potato leaves collected from Yeoncheon (June 2021).Symptoms on the front of the leaves (left), back of the leaves (centre), and stem (right); (b) 14-day-old PDA cultures of five representative fungal species, including Alternaria spp.(far left): Fusarium spp.(second from the left), Boeremia spp.(centre), Stagonosporopsis spp.(right of centre), and Colletotrichum sp.(far right); (c) Spores of each fungus.The scale bar indicate 10 µm.

Figure 2 .
Figure 2. Phylogenetic analysis.Phylogenetic tree constructed based on concatenated sequences of ITS, gapdh, tef1, rpb2, Alt a1, endoPG, and OPA10-2 from 23 strains of Alternaria spp.Reference sequences were retrieved from GenBank (accession numbers shown in Supplementary TableS1).The tree was constructed using the maximum-likelihood method, and bootstrap values (1000 replications) are shown in front of each node.MEGA version X software was used for the analysis.

Figure 2 .
Figure 2. Phylogenetic analysis.Phylogenetic tree constructed based on concatenated sequences of ITS, gapdh, tef1, rpb2, Alt a1, endoPG, and OPA10-2 from 23 strains of Alternaria spp.Reference sequences were retrieved from GenBank (accession numbers shown in Supplementary TableS1).The tree was constructed using the maximum-likelihood method, and bootstrap values (1000 replications) are shown in front of each node.MEGA version X software was used for the analysis.

Figure 4 .
Figure 4. Detached leaf assay without/with wounding and box-plot analysis.(a) Detached leaf assay.Sterilised distilled water was used as the control.Isolates for the virulence test were selected from groups I, II, and III (on the left), which are identical to the groups shown in Figure 2. Box-plot analysis using diseased-leaf-area data from the virulence test (b) without wounding, and (c) with wounding.The use of the same colour in different box plots indicates no significant difference between cultivars.The line inside each box represents the median value.Outliers are shown as dots.Bars indicate the standard error of the means (n = 25).

Figure 4 .
Figure 4. Detached leaf assay without/with wounding and box-plot analysis.(a) Detached leaf assay.Sterilised distilled water was used as the control.Isolates for the virulence test were selected from groups I, II, and III (on the left), which are identical to the groups shown in Figure 2. Box-plot analysis using diseased-leaf-area data from the virulence test (b) without wounding, and (c) with wounding.The use of the same colour in different box plots indicates no significant difference between cultivars.The line inside each box represents the median value.Outliers are shown as dots.Bars indicate the standard error of the means (n = 25).

Table 1 .
Primers used for PCR and sequencing.

Table 2 .
Chemical names, group name, formulation, and final concentration in the medium used in the Alternaria trials.

Table 3 .
List of collected isolates, localities, species, and GenBank accession numbers for ITS region sequences.

Table 4 .
Rates of inhibition of fungal growth by seven selected fungicides tested against Alternaria spp.Data are presented as mean ± SD from three replicates.# Duncan's multiple range test was used to determine significance at the 95% probability level.The presence of the same letters multiple times in a column indicates no significant difference between those results.