Pathological Findings in Gastrointestinal Neoplasms and Polyps in 860 Cats and a Pilot Study on miRNA Analyses
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Histopathology
2.2. Immunohistochemistry
2.3. miRNA Analysis
2.4. Statistics
3. Results
3.1. Total Cat Population with Gastrointestinal Non-Inflammatory Masses (n = 860)
3.2. Lymphoma, n = 679
3.3. Carcinoma, n = 122
3.4. Spindle Cell Tumours, n = 29
3.5. Polyps, n = 23
3.6. Mast Cell Tumours, n = 7
3.7. miRNA Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Antigen (Clone) | Species | Supplier | Dilution | Pre-Treatment |
---|---|---|---|---|
CD3 (F7.2.38) | mouse | Dako 1 #M7254 | 1:100 | EDTA buffer |
CD79a (HM57) | mouse | BioRad 2 #MCA2538GA | 1:3000 | EDTA buffer |
CD20 | rabbit | Epria 3 #RB-9013-P1 | 1:100 | EDTA buffer |
cKit/CD117 | rabbit | Dako 1 #A4502 | 1:150 | EDTA buffer |
Smooth muscle Alpha actin (1A4) | mouse | Dako 1 #M0851 | 1:100 | target retrieval solution buffer |
Glial fibrillary protein | rabbit | Dako 1 #Z0334 | 1:300 | EDTA buffer |
Group | Breed | Age (Years) | Sex |
---|---|---|---|
B-cell lymphoma (n = 6) | 4 DSH, 1 mix, 1 Somali | 4–16 | 1 m, 1 mc, 1 f, 3 fs |
T-cell lymphoma (n = 5) | 5 DSH | 6–14 | 3 mc, 2 fs |
Carcinoma (n = 5) | 4 DSH, 1 Russ. blue | 8–14 | 1 m, 4 mc |
Controls (n = 5) | 3 DSH, 1 BSH, 1 Russ. blue | 1–13 | 2 m, 3 mc |
Site | Breed | Age (Years) | Sex |
---|---|---|---|
Stomach (n = 118) | 74 DSH, 8 Maine coon, 7 BSH, 6 mix, 3 Chartreux, 3 Persian, 3 Russ. blue, 3 Siamese, 3 Thai, 2 Burmese, 2 Norw. forest cat, 4 others | 1–17 median: 10 | 8 m, 48 mc, 10 f, 52 fs |
Small intestine (n = 310) | 233 DSH, 27 mix, 9 Maine coon, 9 Norw. forest cat, 6 Persian, 4 BSH, 4 Siamese, 4 Turkish Angora, 3 Bengal, 3 ELH, 2 ASH, 6 others | 1–18 median: 11 | 22 m, 157 mc, 23 f, 108 fs |
Large intestine (n = 44) | 32 DSH, 4 BSH, 3 mix, 2 Maine coon, 3 others | 1–15 median: 10 | 2 m, 24 mc, 2 f, 16 fs |
Intestine NOS (n = 182) | 123 DSH, 20 mix, 11 BSH, 5 Maine coon, 3 ELH, 2 Bengal, 2 DLH, 2 Siamese, 14 others | 1–16 median: 10 | 27 m, 76 mc, 18 f, 61 fs |
Intestine and stomach (n = 25) | 15 DSH, 2 BSH, 2 mix, 6 others | 4–14 median: 11 | 1 m, 10 mc, 1 f, 13 fs |
Site | Breed | Age (Years) | Sex | Morphology |
---|---|---|---|---|
Stomach (n = 3) | 1 Bengal, 1 Norw. forest cat, 1 Siberian | 9–12 median: 10 | 1 mc, 2 fs | 2 tubular, 1 undiff. |
Small intestine (n = 39) | 26 DSH, 5 mix, 1 Balinese, 1 Bengal, 1 Birman, 1 BSH, 1 ELH, 1 Oriental, 1 Russ. blue, 1 Siamese | 5–19 median: 11 | 7 m, 20 mc, 3 f, 9 fs | 25 acinar (2× bone metaplasia), 4 mucinous, 10 undiff. |
Large intestine (n = 71) | 47 DSH, 8 mix, 6 BSH, 3 Maine coon, 3 Persian, 2 Chartreux, 1 BLH,1 Norw. forest cat | 3–23 median: 11 | 5 m, 33 mc, 12 f, 21 fs | 54 acinar (3× bone metaplasia), 9 undiff., 8 mucinous |
Intestine NOS (n = 9) | 6 DSH, 1 Balinese, 1 Persian, 1 Sphynx | 7–17 median: 12 | 6 mc, 3 fs | 5 acinar, 3 undiff., 1 mucinous |
Tumour | Site | Breed | Age (Years) | Sex |
---|---|---|---|---|
Leiomyosarcoma (n = 8) | 5 small intestine, 1 large intestine, 1 stomach, 1 intestine NOS | 7 DSH, 1 Cornish Rex | 2–15 | 4 mc, 4 fs |
Haemangiosarcoma (n = 4) | 2 large intestine 2 intestine NOS | 3 DSH, 1 Persian | 5–10 | 1 mc, 3 fs |
Leiomyoma (n = 1) | small intestine | DSH | 13 | fs |
Neurofibrosarcoma (n = 1) | large intestine | DSH | 7 | fs |
Spindle cell sarcoma NFD (n = 12) | 6 large intestine, 3 small intestine, 3 stomach | 7 DSH, 3 BSH, 2 mix | 5–16 | 2 m, 5 mc, 3 f, 2 fs |
Pleomorphic sarcoma NOS (n = 3) | 3 intestine NOS | 3 DSH | 12–18 | 1 mc, 1 f, 1 fs |
Site | Breed | Age (Years) | Sex |
---|---|---|---|
Stomach (n = 3) | 1 BSH, 1 DSH, 1 mix | 7–14 | 1 mc, 2 fs |
Small intestine (n = 8) | 8 DSH | 11–16 | 1 m, 5 mc, 2 fs |
Large intestine (n = 12) | 5 DSH, 2 Chartreux, 2 Maine coon, 2 mix, 1 Norw. forest cat | 1–15 | 8 mc, 1 f, 3 fs |
Case No | Sex | Age | Cellular Differentiation | Mitotic Figures/hpf | cKit Expression Pattern |
---|---|---|---|---|---|
1 | mc | 11 | moderate | 3 | diffuse |
2 | fs | 11 | good | 1 | diffuse + stippled |
3 | fs | 13 | sclerosing | 1 | diffuse |
4 | fs | 11 | good | 1 | diffuse + stippled |
5 | mc | 10 | pleomorphic | 10 | diffuse |
6 | mc | 9 | pleomorphic | 2 | diffuse |
7 | mc | 16 | poor (histiocytic) | 1 | diffuse + stippled |
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Kehl, A.; Törner, K.; Jordan, A.; Lorenz, M.; Schwittlick, U.; Conrad, D.; Steiger, K.; Schusser, B.; Aupperle-Lellbach, H. Pathological Findings in Gastrointestinal Neoplasms and Polyps in 860 Cats and a Pilot Study on miRNA Analyses. Vet. Sci. 2022, 9, 477. https://doi.org/10.3390/vetsci9090477
Kehl A, Törner K, Jordan A, Lorenz M, Schwittlick U, Conrad D, Steiger K, Schusser B, Aupperle-Lellbach H. Pathological Findings in Gastrointestinal Neoplasms and Polyps in 860 Cats and a Pilot Study on miRNA Analyses. Veterinary Sciences. 2022; 9(9):477. https://doi.org/10.3390/vetsci9090477
Chicago/Turabian StyleKehl, Alexandra, Katrin Törner, Annemarie Jordan, Mareike Lorenz, Ulrike Schwittlick, David Conrad, Katja Steiger, Benjamin Schusser, and Heike Aupperle-Lellbach. 2022. "Pathological Findings in Gastrointestinal Neoplasms and Polyps in 860 Cats and a Pilot Study on miRNA Analyses" Veterinary Sciences 9, no. 9: 477. https://doi.org/10.3390/vetsci9090477