1. Introduction
2. Materials and Methods
- 5′GGGGATCCTGGAACACCATGTTAGACGCAG3′ (Forward)
- 5′GGGAATTCTGCAGTTACCTTTAACACAGAG3′ (Reverse) for Ch1;
- 5′GGGCTGCAGATTCTAGTCAGCCTGATTGA3′ (Forward)
- 5′GGGGATCCTGACACCATGAGGGGGGCA3′ (Reverse) for Ch2.
3. Results
4. Discussion
5. Conclusions
Author Contributions
Conflicts of Interest
References
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Cat No. | Mutation Ch1 X62477 | Effect | Mutation Ch2 NM_001048154.1 | Effect | Mutation Ch2 X62478 | Effect | Fel d 1 (µg/mL) |
---|---|---|---|---|---|---|---|
g.138G > T | p.A13S | c.260_265delAGCTCC | p.S85_S86del | g.2231A > G | p.N85S | ||
21 | − | + | − | unknown | |||
37 | + | + | + | 0.48 | |||
38 | − | + | - | 2.19 | |||
39 | Not amplified | + | − | 1.66 |
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