Prebiotic Potential and Anti-Inflammatory Activity of Soluble Polysaccharides Obtained from Soybean Residue

In the present study, we assessed the extraction of low molecular weight soluble polysaccharides (MESP) from soybean by-products using microwave-assisted enzymatic technology and proposed the chemical structure of MESP using Fourier transform-infrared spectroscopy, gas chromatography, and 1H and 13C nuclear magnetic resonance spectrum analysis. The results suggested that MESP mainly comprised arabinose, rhamnose, and glucuronic acid with (1→4) glycosidic linkages in the backbone. Compared with inulin, MESP was found to selectively stimulate the growth of Lactobacillus probiotics. Moreover, the results of in vitro fermentation indicated that MESP significantly increased the concentrations of both acetate and butyrate (p < 0.05). MESP were treated on lipopolysaccharide (LPS)-stimulated RAW264.7 cells to determine the anti-inflammatory effect in vitro. It was observed that MESP inhibited nitric oxide, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10 production. Furthermore, Western blotting results indicated that MESP significantly attenuated LPS-induced downregulation of phosphorylation levels of Janus kinase 2 (JAK2) and signal transducer and activator of transcription 3 (STAT3) in macrophages. The underlying mechanism might involve inhibition of the expression of pro-inflammatory cytokines, presumably via JAK2/STAT3 pathway. Collectively, the results of our study paved way for the production of MESP, which may be potentially used as nutraceutical ingredients for prebiotics and anti-inflammatory agents, from soybean residue.


Introduction
Soy (Glycine max) is an economical crop that has been cultivated in eastern Asian countries since ancient times, and more recently, is increasingly used in the Western countries [1]. Soybean is used for human consumption, since it is an abundant source of protein and oil. Soymilk consumption has increased in most countries; however, increased soymilk production has been continuously accompanied by soybean by-product accumulation. The residue left from soybean after aqueous extraction from soymilk production is rich in insoluble fiber (55%) and remaining protein (30%) [2]. Several studies have been conducted on soybean by-products to identify different methodologies to minimize the economic and environmental load of food production.
For decades, research has revealed that polysaccharides possess immense health beneficial physiological effects including immunomodulatory [3], antiviral [4], and antioxidant activities [5]. Soybean polysaccharide and its derivatives were reported to possess hypolipemic, hypocholesterolemic, anti-inflammatory, antidiabetic, antiobesity, anticardiovascular disease activities, and these derivatives oligosaccharide, colored materials, and some small molecule materials. The insoluble parts were separated via centrifugation and dried until its weight was constant. The microwave extraction was performed as previously described by Le, Golokhvast, Yang, and Sun [16] with minor modifications. The pretreated powder of 10 g was accurately weighed and was immersed in 200 mL deionized water (D 2 O) (1:10 w/v) for 1 h in a 250 mL flat-bottomed flask. The mixture was hydrolyzed for 30 min using a cocktail enzyme (pectinase: cellulase = 1:1, mixed enzymes: powder = 2.0%, w/v) under the microwave irradiation power of 700 W. Thereafter, the suspension was centrifuged and concentrated to reach 20 mL by rotary evaporation under reduced pressure. Next, the mixture was precipitated with 80% (v/v) ethanol) at 4 • C overnight. The crude polysaccharide was obtained via centrifugation, deproteinized by the Sevag reagent (chloroform: n-butyl alcohol = 4:1, v/v) to remove the associated proteins, and finally lyophilized through freeze drying process. The crude polysaccharide was redissolved in distilled water and fractionated through an activated Cellulose DEAE-52 (5.0 × 30 cm) column pre-equilibrated with distilled water and was then stepwise eluted with distilled water and 0.5 M NaCl at a flow rate of 1.0 mL/min (10 mL/tube). Each fraction was evaluated at 490 nm by phenol sulfuric acid to detect the compounds. The main fraction was extensively dialyzed against ultrapure water at 4 • C for 48 h (molecular weight cut-off: 3.5 kDa), and lyophilized to obtain white pure polysaccharide, namely MESP, for further analyses.

Characterization of MESP
Total polysaccharide content was measure by the phenol-sulfuric acid colorimetric method at 621 nm using D-glucose as a standard. The molecular weight of MESP was measured according to a method described by Zheng et al. [17]. Monosaccharide composition of MESP was determined via a gas chromatography-mass spectrometer (6890/5973N-GC/MSD, Agilent Technologies, Santa Clara, CA, USA) after hydrolysis with 3 M trifluoroacetic acid at 100 • C for 3 h, and then the MESP were passed through a 0.45 µm syringe filter before loading on a capillary column HP-5 ms (30 m × 0.25 mm × 0.25 µm) at 25 • C. VERTEX 70 v Fourier transform infrared spectrometer (Bruker, Germany) was used for FT-IR measurements. A potassium bromide disc that contains 1% of MESP was recorded in the frequency range of 4000-400 cm -1 . MESP (30 mg) was maintained over P 2 O 5 under vacuum for several days and then dissolved in D 2 O. The solid-state 1 H and 13 C nuclear magnetic resonance (NMR) spectra were recorded at 25 • C on a Varian Inova 500 and 600 MHz spectrometers (Varian, Walnut Creek, CA, USA), which were operated at 400 MHz and 101 MHz for 1 H and 13 C NMR, respectively.

Stimulation of Probiotic Growth
The prebiotic activity of MESP was evaluated by a method described in a previous study with minor modifications [18]. MRS carbohydrate-free broth (MB cell, Los Angeles, CA, USA) was used as the basal medium, and MESP and inulin (positive prebiotic control) were used as the individual carbon sources. Three Lactobacillus strains, including L. rhamnosus GG, L. plantarum, and L. brevis, were precultured in MRS broth, centrifuged and diluted in basal MRS medium to attain 1 × 10 7 colony forming units (CFU)/mL. The MRS carbohydrate-free broth containing 1% carbon source (MESP or inulin) was dispensed as 200 µL aliquots into in 96-well U-shaped-bottom microplate; thereafter, 20 µL of logarithmic culture of each probiotic strains was added for fermentation. The growth of the probiotics was evaluated after 24 h of incubation using an ELISA reader (BioTek Instruments, Winooski, VT, USA) at a wavelength of 600 nm.

Short Chain Fatty Acid (SCFA) Analysis
In vitro fermentation was performed according to a previously described method [19] with certain modifications. Fresh feces samples were obtained from three healthy individuals (2 female, 1 male, aged 22-29 years), who had not been treated with antibiotics in the last 3 months. Feces were homogenized with 0.1 M anaerobic phosphate-buffered saline (pH 7.0) using a Bead Ruptor Homogenizer (Omni International, NW Kennesaw, GA, USA) to make 10% (w/v) slurries. The basic medium comprised the followings components (per liter): 0.45 g KH 2 PO 4 ; 0.45 g K 2 HPO 4 ; 0.05 g NaCl; 0.064 g KCl; 1.0 g MgSO 4 ·7H 2 O; 0.5 g cysteine-HCl; 0.5 g bile salts, 0.05 g hemin; 0.001 g resazurin; 2.0 mL Tween 80; 0.01 mL vitamin K, with or without supplement of 8.0 g each of MESP or inulin. The fermentation was carried out in an anaerobic chamber at 37 • C for 24 h. After fermentation, the culture (1 mL) was dispensed into a sterile Eppendorf tube (1.5 mL) and centrifuged at 13,000× g for 10 min to collect the supernatant. Next, the supernatants were filtered using organic phase microfiltration membrane (0.22 mm) and analyzed by Agilent 1100 Series (Agilent Technologies, Inc., Santa Clara, CA, USA) with a DAD detector at 210 nm equipped with a calcium-loaded Aminex-HPX-87C column (Bio-Rad Corp., Richmond, CA, USA).

Cytotoxicity Assay
We examined the cytotoxicity of MESP on RAW 264.7 murine macrophage cells by growing them in Dulbecco's modified eagle's medium supplemented with combination of penicillin and streptomycin (final concentration of 100 units/mL and 100 µg/mL, respectively), along with 10% fetal bovine serum at 37 • C, with 5% CO 2 supply. As the cells reached 80% confluence, they were treated with different concentrations of MESP (5-1280 µg/mL) for 24 h. The cell vitality was measured using methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay using microplate reader (F50, Tecan, Mannedorf, Switzerland) via spectrophotometry at 570 nm.

Measurement of NO and Cytokines
To evaluate anti-inflammatory potential of MESP, RAW264.7 cells (5 × 10 5 cells/mL) were seeded in a 24-well plate and adding MESP at various concentrations in the presence of LPS (1 µg/mL) in 5% CO 2 at 37 • C for 24 h. The NO, TNF-α, IL-6, IL-10, and IL-1β concentrations in the supernatant were measured by Griess and ELISA assays according to the manufacturer's instructions.

Western Blot Analysis
RAW264.7 cells were rinsed with cold 1× phosphate buffer saline and detached with EDTA-free 0.25% trypsin, followed by homogenization using RIPA lysis buffer (Sigma-Aldrich, St. Louis, MO, USA). After quantitative determination of the protein content by BCA assay kit, equal quantities of total proteins were separated by denaturing them via 10% SDS-PAGE and then transferred to PVDF membranes (GE Healthcare, Buckinghamshire, UK). The membranes were then incubated in Tris-buffered saline containing 0.1% (v/v) Tween-20 (TBST) and 5% skim milk at 37 • C for 3 h, and the specific primary antibodies were incubated at 4 • C overnight. After several washes with TBST (0.075%), they were incubated with anti-rabbit horseradish peroxidase-conjugated secondary antibodies for 1 h at room temperature. The targeted protein blots were developed using an enhanced chemiluminescence (ECL) kit (ECL-plus, Thermo Scientific, USA) and detected by using a Chemiluminometer (CLINX Scientific Instrument Co., Ltd., Shanghai, China).

Statistical Analysis
All results were performed in triplicate and expressed as mean ± standard deviation. Analysis of variance and Student's t-test were analyzed using the SPSS software (IBM, SPSS 22.0, Chicago, IL, USA) at p < 0.05 and p < 0.01.   Gas chromatography (GC) analysis of the monosaccharides (

Structural Characterization
The integrated structure of MESP was further assessed via FT-IR and 1 H and 13 C NMR. The typical FT-IR spectrum of MESP in the 400-4000 cm −1 range is presented in Figure 2a. The graft MESP revealed strong stretching peak at 3397.8 cm −1 corresponding to the hydroxyl groups (O-H) [20]. The weak absorption peak at 2919.2 cm −1 was characteristic of C-H asymmetric stretching vibration [21]. The bands at 1736.2 and 1629.6 cm −1 might be due to the nonsymmetrical vibration of C=O in the ester group [22]. Moreover, the absorption signals at around 1473.3 and 1074.1 cm −1 indicated the presence of residual water (OH -). Furthermore, the signal observed at 1256.3 cm −1 was attributed to the stretching vibration of C-O. Collectively, FT-IR analysis suggested that MESP possessed typical sugar groups. Gas chromatography (GC) analysis of the monosaccharides (Table 1) revealed that MESP comprised arabinose, galacturonic acid, rhamnose, xylose, galactose, glucose, and fucose at a molar ratio of 27.4:18.1:11.41:5.71:2.53:1.53:1.32, indicating that arabinose, galacturonic acid, and rhamnose were the main monosaccharide components of low molecular weight soluble polysaccharides (MESP).

Structural Characterization
The integrated structure of MESP was further assessed via FT-IR and 1 H and 13 C NMR. The typical FT-IR spectrum of MESP in the 400-4000 cm −1 range is presented in Figure 2a. The graft MESP revealed strong stretching peak at 3397.8 cm −1 corresponding to the hydroxyl groups (O-H) [20]. The weak absorption peak at 2919.2 cm −1 was characteristic of C-H asymmetric stretching vibration [21]. The bands at 1736.2 and 1629.6 cm −1 might be due to the nonsymmetrical vibration of C=O in the ester group [22]. Moreover, the absorption signals at around 1473.3 and 1074.1 cm −1 indicated the presence of residual water (OH -). Furthermore, the signal observed at 1256.3 cm −1 was attributed to the stretching vibration of C-O. Collectively, FT-IR analysis suggested that MESP possessed typical sugar groups.
The 1 H and 13 C NMR spectra, illustrated in Figure 2b,c, confirmed the attribution and structural characterization of MESP. Based on the data in existing literature, the signal of five anomeric protons at δ3.18-4.18 was identified as the zone of accumulation of protons from α-D-GalAresidues, whereas that at δ1.21-4.03 was assigned to α-L-Rhap - [8]. The 13 C NMR spectrum of MESP showed no signal at low field from 160 to 180 ppm, indicating that MESP did not contain uronic acid. Chemical shifts of MESP are summarized in Table 2. According to the corresponding chemical shifts in existing literature, MESP structure was proposed, as shown in Figure 2d.
weak absorption peak at 2919.2 cm −1 was characteristic of C-H asymmetric stretching vibration [21]. The bands at 1736.2 and 1629.6 cm −1 might be due to the nonsymmetrical vibration of C=O in the ester group [22]. Moreover, the absorption signals at around 1473.3 and 1074.1 cm −1 indicated the presence of residual water (OH -). Furthermore, the signal observed at 1256.3 cm −1 was attributed to the stretching vibration of C-O. Collectively, FT-IR analysis suggested that MESP possessed typical sugar groups. The 1 H and 13 C NMR spectra, illustrated in Figure 2b,c, confirmed the attribution and structural characterization of MESP. Based on the data in existing literature, the signal of five anomeric protons at δ3.18-4.18 was identified as the zone of accumulation of protons from α-D-GalAresidues, whereas that at δ1.21-4.03 was assigned to α-L-Rhap - [8]. The 13 C NMR spectrum of MESP showed no signal at low field from 160 to 180 ppm, indicating that MESP did not contain uronic acid. Chemical shifts of MESP are summarized in Table 2. According to the corresponding chemical shifts in existing literature, MESP structure was proposed, as shown in Figure 2d.

MESP Stimulated Probiotic Growth
The number of all tested Lactobacillus strains treated with MESP or inulin was remarkably increased (Figure 3a), indicating that all Lactobacillus strains could utilize MESP and inulin. Lactobacillus exhibited an outstanding growth rate when MESP, in contrast to inulin, was used as the carbon source.

MESP Stimulated Probiotic Growth
The number of all tested Lactobacillus strains treated with MESP or inulin was remarkably increased (Figure 3a), indicating that all Lactobacillus strains could utilize MESP and inulin. Lactobacillus exhibited an outstanding growth rate when MESP, in contrast to inulin, was used as the carbon source.

Effect of MESP on SCFA Production
The pH of the cecum is lower than that of the ileum, which in turn affects the growth inhibition of pH-sensitive pathogenic bacteria, thereby altering gut microbiota composition and promoting host health. After in vitro fermentation, the concentrations of total short chain fatty acids (SCFAs), including acetic, butyric, and propionic acids, were significantly higher (p < 0.05) in the MESP group than those in the control group (Table 3), indicating that MESP enhanced SCFA production in the gut model. A comparison of the results revealed that the concentrations of total SCFAs were significantly higher (p < 0.05) after MESP treatment than those after inulin treatment. Acetic acid concentration was significantly increased (p < 0.05).

Effect of MESP on SCFA Production
The pH of the cecum is lower than that of the ileum, which in turn affects the growth inhibition of pH-sensitive pathogenic bacteria, thereby altering gut microbiota composition and promoting host health. After in vitro fermentation, the concentrations of total short chain fatty acids (SCFAs), including acetic, butyric, and propionic acids, were significantly higher (p < 0.05) in the MESP group than those in the control group (Table 3), indicating that MESP enhanced SCFA production in the gut model. A comparison of the results revealed that the concentrations of total SCFAs were significantly higher (p < 0.05) after MESP treatment than those after inulin treatment. Acetic acid concentration was significantly increased (p < 0.05).

Effects of MESP on RAW264.7 Cell Proliferation
We analyzed the safe concentration of MESP on RAW 264.7 macrophage, with various MESP concentrations wherein cell viability was determined by MTT assay. We observed that MESP at concentrations 5-1280 µg/mL did not affect cell growth in a dose-dependent manner. (Figure 3b).

Effects of MESP on NO Production in LPS-Stimulated RAW264.7 Cells
The NO levels were markedly induced after treatment with LPS alone, whereas they were remarkably inhibited by MESP pretreatment in a dose-dependent manner ( Figure 3c). As 160 µg/mL MESP did not present any cytotoxic effect, we propose the possibility that inhibition of NO production was not linked to the cytotoxic effects on RAW264.7 cells.

Effects of MESP on RAW264.7 Cell Proliferation
We analyzed the safe concentration of MESP on RAW 264.7 macrophage, with various MESP concentrations wherein cell viability was determined by MTT assay. We observed that MESP at concentrations 5-1280 μg/mL did not affect cell growth in a dose-dependent manner. (Figure 3b).

Effects of MESP on NO Production in LPS-Stimulated RAW264.7 Cells
The NO levels were markedly induced after treatment with LPS alone, whereas they were remarkably inhibited by MESP pretreatment in a dose-dependent manner ( Figure 3c). As 160 μg/mL MESP did not present any cytotoxic effect, we propose the possibility that inhibition of NO production was not linked to the cytotoxic effects on RAW264.7 cells.

MESP Blocked the LPS-Triggered Inflammatory Response via the JAK2/STAT3 Pathway
We revealed that LPS induced JAK2 and STAT3 phosphorylation ( Figure 5). Next, we explored the role of MESP in JAK2 and STAT3 regulation. As illustrated in Figure 5, MESP could significantly suppress phosphorylated (LPS-induced) JAK2/STAT3, indicating that MESP exerted antiinflammatory effects through the JAK2/STAT3 signaling pathway.

Discussion
In the present study, crude polysaccharide was extracted from soybean residue by MAEE extraction and then fractionated using the Cellulose DEAE-Sephadex A-25 column. The extraction yield was 73.8%, which is comparable to that (previously reported value ranging from 7.09-85%) obtained using alkaline hydrogen peroxide, enzymatic hydrolysis, autoclaving, fermentation, and ultrasonic-assisted extraction [23][24][25]. In MAEE, the extraction time was shorter than that in microwave-assisted extraction (0.5-2 h), whereas the extraction temperature was lower than that for autoclaving (121 °C); moreover, enzyme loading of MAEE extraction was lower than that of enzymatic hydrolysis using cellulase and pectinases, thereby suggesting that MAEE extraction was more efficient and eco-friendly. In a previous study, numerous heterogeneous polysaccharides isolated from soybean residue (okara) presented monosaccharide compositions comprising mainly

MESP Blocked the LPS-Triggered Inflammatory Response via the JAK2/STAT3 Pathway
We revealed that LPS induced JAK2 and STAT3 phosphorylation ( Figure 5). Next, we explored the role of MESP in JAK2 and STAT3 regulation. As illustrated in Figure 5, MESP could significantly suppress phosphorylated (LPS-induced) JAK2/STAT3, indicating that MESP exerted anti-inflammatory effects through the JAK2/STAT3 signaling pathway.

MESP Blocked the LPS-Triggered Inflammatory Response via the JAK2/STAT3 Pathway
We revealed that LPS induced JAK2 and STAT3 phosphorylation ( Figure 5). Next, we explored the role of MESP in JAK2 and STAT3 regulation. As illustrated in Figure 5, MESP could significantly suppress phosphorylated (LPS-induced) JAK2/STAT3, indicating that MESP exerted antiinflammatory effects through the JAK2/STAT3 signaling pathway.

Discussion
In the present study, crude polysaccharide was extracted from soybean residue by MAEE extraction and then fractionated using the Cellulose DEAE-Sephadex A-25 column. The extraction yield was 73.8%, which is comparable to that (previously reported value ranging from 7.09-85%) obtained using alkaline hydrogen peroxide, enzymatic hydrolysis, autoclaving, fermentation, and ultrasonic-assisted extraction [23][24][25]. In MAEE, the extraction time was shorter than that in microwave-assisted extraction (0.5-2 h), whereas the extraction temperature was lower than that for autoclaving (121 °C); moreover, enzyme loading of MAEE extraction was lower than that of enzymatic hydrolysis using cellulase and pectinases, thereby suggesting that MAEE extraction was more efficient and eco-friendly. In a previous study, numerous heterogeneous polysaccharides isolated from soybean residue (okara) presented monosaccharide compositions comprising mainly Figure 5. Inhibitory effects of MESP on JAK2/STAT3 pathway activation. Immunoblot analysis of JAK2 and STAT3 protein levels (a) and quantification of protein levels (b) normalized to β-actin. ## p < 0.01, compared to the control group; * p < 0.05, ** p < 0.01, compared to the LPS treated group.

Discussion
In the present study, crude polysaccharide was extracted from soybean residue by MAEE extraction and then fractionated using the Cellulose DEAE-Sephadex A-25 column. The extraction yield was 73.8%, which is comparable to that (previously reported value ranging from 7.09-85%) obtained using alkaline hydrogen peroxide, enzymatic hydrolysis, autoclaving, fermentation, and ultrasonic-assisted extraction [23][24][25]. In MAEE, the extraction time was shorter than that in microwave-assisted extraction (0.5-2 h), whereas the extraction temperature was lower than that for autoclaving (121 • C); moreover, enzyme loading of MAEE extraction was lower than that of enzymatic hydrolysis using cellulase and pectinases, thereby suggesting that MAEE extraction was more efficient and eco-friendly. In a previous study, numerous heterogeneous polysaccharides isolated from soybean residue (okara) presented monosaccharide compositions comprising mainly galacturonic acid, galactose, arabinose, xylose, fucose, and rhamnose [2,26]. Furthermore, enzyme treatment increased arabinose content in polysaccharides obtained from tea leaf and pulp [27,28], and in accordance with our results. This demonstrated that MESP was a pectin-like polysaccharide with rhamnose and galacturonic acid forming its backbone.
To evaluate the growth stimulating effects of MESP on probiotics, MESP was used as the single carbon source for Lactobacillus spp. cultivation. Meanwhile, the medium without a carbon source was used as a negative control, whereas inulin (commercial prebiotic) was used as a positive control to compare its effects with those of MESP. Our results were in accordance to those of previous in vitro and in vivo studies, which revealed that soybean oligosaccharides and soluble polysaccharides could stimulate Lactobacillus and Bifidobacterium growth [29][30][31]. Soybean soluble polysaccharides, which are rich in GalA and Ara, were reported to stimulate Lactobacillus fermentum and L. plantarum growth by 49.5 and 5.25 times, respectively [29]. A similar result was observed, demonstrating that consumption of high-dose soybean oligosaccharides could promote Bifidobacterium and Lactobacillus proliferation in a mouse model [32]. This indicated that MESP was a good substrate for facilitating probiotic growth.
SCFAs are the main end-products obtained fermentation of dietary fiber by gut microbes in the colons [19]. This increase was consistent with the change in the number of Lactobacillus spp. (known as lactate producers). Enriched acetate production has been reported in numerous inulin intervention studies [14,29]. Moreover, a similar result has been documented when soybean oligosaccharides were used. Zhou, et al. [33] found that the fermentation of mini-pig-supplemented soybean oligosaccharides increased acetic, propionic, and butyrate acid production, and this could be corrected by increasing the number of Lactobacillus spp. Our results indicated that MESP exerted better effects on SCFA production and richness than inulin, and this might be due to the more diverse monosaccharide compositions of MESP.
Macrophages are crucial immunocytes and play a pivotal role in protecting the host from certain pathogens and maintaining homeostasis [34]. Our result revealed that MESP exerted no toxicity toward RAW264.7 cells, and this was in accordance with the results of previous studies [32,35]. Moreover, soybean oligosaccharide was considered "generally recognized as safe" material in the United States. To estimate the suppressive effects on NO, pro-inflammatory mediator in LPS-induced inflammation, RAW264.7 cells were treated with MESP at serial concentrations for 4 h before treatment with LPS (1 µg/mL). These data suggested that MESP exerts anti-inflammatory activity towards RAW264.7 cells. To determine the underlying anti-inflammatory mechanisms of MESP, we evaluated the production of inflammatory cytokines, which are crucially involved in the inflammatory response. The immunomodulatory capacities were affected by the different monosaccharide compositions of the MESP, and this was in accordance with the results of a previous study [35]. Furthermore, the (1→4) glycosidic bond, which is the main type of linkage formed in MESP, might partly be responsible for the immunoregulatory activity of MESP [36,37]. A previous study showed that soybean curd residue at higher concentrations (800-1600 µg/mL) significantly downregulated LPS-induced TNF-α and IL-1β protein expression [38]. Additionally, admixtures of soybean soluble polysaccharides and genistein reduced IL-1, IL-6, and TNF-α levels in high-dose L-carnitine-fed mice [39]. Recent studies have confirmed the importance of JAK2/STAT3 in the macrophage inflammatory response [40,41]. STAT3 is a protein that mediates the expression of numerous cytokines such as IL-6 to promote inflammation. p-STAT3 can subsequently translocate into the nucleus and bind to DNA response elements, and it is generally activated by regulating the activity of the cytoplasmic tyrosine kinase JAK2 [42]. Further evidence that the JAK2/STAT3 signaling pathway could be activated by a series of cytokines, including TNF-α, IL-1β, and IL-6, has been presented [43].

Conclusions
The present study revealed that the use of MAE extraction, followed by anion exchange chromatography, allowed the production of low molecular weight (3.32 kDa) MESP from soybean residue. MESP mainly comprised galacturonic acid, rhamnose, and arabinose with copious (1→4) glycosidic linkages in its backbone. MESP exhibited remarkable in vitro prebiotic properties, including stimulation of Lactobacillus growth and SCFA production. Furthermore, MESP possessed potential anti-inflammatory activity that might be mediated by inhibiting NO, IL-6, IL-1β, IL-10, and TNF-α production by suppressing the JAK2/STAT3 pathway in LPS-stimulated RAW264.7 cells.