Amplification of Vitamin D2 in the White Button Mushroom (Agaricus bisporus) by UV-B Irradiation and Jet-Milling for Its Potential Use as a Functional Ingredient

The objective of this study was to amplify vitamin D2 in white button mushrooms using ultraviolet (UV-B) irradiation and to prepare a vitamin D2-fortified superfine mushroom powder through jet milling. Mushrooms irradiated with UV-B for 30 min had a vitamin D2 concentration of 8.19 μg/g, an amount about 400 times greater than that of the control (0.02 μg/g). The vitamin D2-fortified mushrooms were then freeze-dried and conventionally ground or jet-milled to obtain coarse (Dv50 = 231 μm), fine (Dv50 = 106.3 μm), and superfine (Dv50 = 7.1 μm) powders. The vitamin D2 content was retained during the preparation of the powders. The physical characteristics were evaluated by scanning electron microscopy and hydration properties. The superfine powder of vitamin D2-amplified mushrooms was suitable for use as a functional ingredient because its roughness was significantly reduced, and it had a neutral aroma and taste as determined by descriptive analysis.


Introduction
Ergocalciferol (vitamin D 2 ) is a synthetic form of vitamin D that can be formed by ultraviolet (UV) irradiation from the plant steroid ergosterol [1]. Vitamin D is also known to prevent some diseases, such as osteomalacia and rickets [2]. Disorders caused by vitamin D deficiency are frequently reported worldwide [3]. Mushrooms contain large quantities of ergosterol, which is converted to vitamin D 2 when exposed to sunlight or ultraviolet light. The conversion of vitamin D was found to be dependent on the applied wavelength. The conversion was most effective under ultraviolet-B (UV-B) than ultraviolet-C (UV-C) or ultraviolet-A(UV-A) [4][5][6]. Vitamin D is commonly contained in animal-based foods, but mushrooms contain a high concentration of ergosterol, which can be converted to vitamin D [7]. Currently, the most cultivated and consumed mushroom is button mushrooms (Agaricus bisporus), which is very popular in both the East and the West [4]. The processing of UV-B-irradiated white button mushrooms is challenging because discoloration occurs during UV-B irradiation, and mushrooms decay immediately due to their high water content. An additional process is needed to extend the shelf life, and drying is frequently used to prolong the shelf life of mushrooms [8]. Dried mushrooms can be further processed into powder form, which can be added to various foods as a functional ingredient with unique flavors [9]. However, the unique flavor and fibrous texture of mushrooms limit the use of mushrooms as food ingredients because many people dislike the fibrous texture and flavor of mushrooms.
Particle size determines the organoleptic properties of a powder. Roughness is directly related to the particle size of the raw material, and the overall acceptance of a fiber-enriched layer cake decreases with an increase in fiber particle size [10]. The small powder size has a corresponding large surface area, and thus water absorption rate and solubility are high [11]. Particle sizes of the raw materials also affect the hydration properties as well as the extractability of food components [12,13]. Previous studies have shown that superfine mushroom powder has remarkable fluidity, water holding capacity and water solubility and is suitable for making instant foods [13,14]. Jet milling is a novel size reduction process, which creates micro and nanoscale particles with minimal thermal impact on food materials [15]. Jet milling is classified as impact milling. Particles are accelerated in a high-velocity air or gas stream, and size reduction is the result of inter-particle collisions or impacts against a solid surface. The final particle size produced by this method is very much dependent on the material being processed, with processing possible even at the 1000 nm scale [16,17].
The objectives of this study were to optimize the formation of vitamin D 2 in white button mushroom through UV-B irradiation and to determine the effect of the superfine grinding process on the vitamin D 2 concentration and physical properties of mushroom powders.

Materials
Freshly harvested white button mushroom (Agaricus bisporus) samples were obtained from a local mushroom farm (Nonsan, Korea) and stored at 4 • C before use. The samples were used in experiments within three days of storage.

UV-B Irradiation of Mushrooms
The UV-B (ultraviolet-B) irradiation of mushrooms was performed using a UV-B irradiator (Ilsinnafyu, Seongnam, Korea) at room temperature. The irradiator was equipped with four Phillips UV-B lamps (TL40W/12; Phillips, Amsterdam, The Netherlands); the irradiation height was 7 cm. The UV-B output was about 12.5 W/m 2 . The mushrooms were sliced to an average thickness of 4 mm, and the sliced mushrooms have a moisture content of about 88%. The sliced mushrooms were placed on shelves and exposed to UV-B radiation for 10, 20, 30, 60 and 90 min. Five hundred grams of sliced mushrooms were used for each treatment. The irradiated samples were freeze-dried (LP 20; Ilshinbiobase, Dongducheon, Korea) and stored airtight in aluminum foil bags to protect against additional UV exposure.

Vitamin D 2 and Ergosterol Analysis by High-Performance Liquid Chromatography (HPLC)
The vitamin D 2 and ergosterol concentrations were determined using a method developed in a previous study, with some modifications [18]. A mushroom sample (1 g) was weighed into a 250 mL flask and then mixed with 1 g of L-ascorbic acid (Duksan Pure Chemicals Co., Ansan, Korea), 25 mL of 55% potassium hydroxide (Duksan Pure Chemicals Co.), 50 mL of 99% ethanol (Burdick & Jackson, Muskegon, MI, USA). The mixture was thoroughly shaken. The saponification was conducted for 30 min under reflux at 85 • C. The mixture was cooled immediately and transferred into a separatory funnel. The extraction was conducted with 30 mL of n-hexane (Daejung Chemicals & Metals, Siheung, Korea) and 10 mL of de-ionized water, and this procedure was repeated twice. The pooled organic layer was washed three times, with 25 mL of de-ionized water until it was neutralized. The organic layer was transferred into a round flask, rotary-evaporated to dryness at 50 • C, and immediately re-dissolved in 2 mL of a solution of isopropyl alcohol, methanol and acetonitrile (2:1:3, v/v). The samples were passed through a filter (PTFE, 13 mm; Whatman International, Ltd., Maidstone, UK), with a pore size of 0.45 µm. Table 1 shows the HPLC system conditions. A 20 µL sample volume was injected into an HPLC system (LC-4000 series; Jasco, Tokyo, Japan) and eluted through a reverse phase C18 column (Polaris 4.6 × 250 mm; Agilent, Santa Clara, CA, USA). The mobile phase was methanol/acetonitrile (75:25, v/v) at a flow rate of 1 mL/min. The detection wavelength of the UV-vis detector (UV-4075; Jasco, Tokyo, Japan) was at 245 nm. The content of vitamin D 2 and ergosterol were calculated on the basis of the calibration curve of authentic vitamin D 2 standard (Merck, Darmstadt, Germany) and ergosterol standard (Merck, Darmstadt, Germany).

Preparation of Coarse, Fine and Superfine Powders from Vitamin D 2 -Amplified Mushrooms
Mushrooms were UV-B irradiated for the optimal time of 30 min (determined as described in Section 3.2) to convert ergosterol into vitamin D 2 . The irradiated samples were freeze-dried (LP 20; Ilshinbiobase, Dongducheon, Korea) and thoroughly blended with a laboratory mixer. The resulting powder was serially sieved with 150 and 63 µm testing sieves (Nonaka Rikaki, Chiyoda, Japan) using a sieve shaker (EML200; Haver and Boecker, Oelde, Germany). The retained powders over the 150 µm sieve were classified as the coarse mushroom powder with vitamin D 2 . The 150 µm sieve passed powders, but retained over the 63 µm sieve were classified as the fine mushroom powder with vitamin D 2 . The blended mushroom powder was further milled by a fluidized-bed jet mill (CGS 10; NETZSCH, Selb, Germany). The milling pressure was 7 bars, and the classifier was set to 12,000 rpm. The jet-milled powder was collected as the superfine mushroom powder with vitamin D 2 . All powder samples were airtight packaged and stored at −20 • C before use.

Particle Size Distribution
The particle size of the mushroom powder was measured by a particle size analyzer (Mastersizer 3000; Malvern Instrument Limited, Worcestershire, UK), using the dry method, with a particle size absorption index of 0.1, particle refractive index of 1.53 and dispersant refractive index of 1.0.

Scanning Electron Microscopy (SEM)
The microstructure of the mushroom powder was examined by a scanning electron microscope (S-3400 N; Hitachi, Tokyo, Japan). Samples were affixed to carbon tape and coated with platinum-lead (Pt-Pb) before observation. The microstructure of the mushroom powder was observed under magnifications of 300×, 500× and 1000×.

Hydration Properties
The water holding capacity of the powder was measured according to a previously reported method [14], with some modifications. The mushroom powder (M 1 , 0.5 g) was poured into a 50 mL centrifuge tube (M). Then, 20 mL of distilled water was added. The mixture was incubated at 60 • C for 30 min. The mixture was cooled in ice for 30 min. The cooled mixtures were then centrifuged at 10,000 rpm for 20 min. After the supernatant was removed, the weight of the residue powder and centrifuge tube (M 2 ) was recorded. Water holding capacity (g/g) was expressed by the following Equation (1): Water holding capacity (g/g) = (M 1 (g)/(M 2 (g) − M (g)).
(1) The swelling capacity of the powder was measured as per a previous report with slight modification [14]. The mushroom powder (1 g) was transferred into a graduated falcon tube, and the bed volume was measured. Then 10 mL of distilled water was added and shaken. The created dispersion by shaken was incubated at 25 • C for 24 h. The final volume of the wetted powder was recorded. Swelling capacity (mL/g) was expressed by the following Equation (2): Swelling capacity (mL/g) = (Final volume (mL) − Initial volume (mL))/Sample (g). ( The water solubility index was determined according to a previously reported method [14], with some modifications. The mushroom powder (W 1 , 0.5 g) was dispersed in 25 mL of distilled water and poured into a 50 mL falcon tube. The sample tubes were incubated in a water bath at 80 • C for 30 min and then centrifuged at 10,000 rpm for 20 min. The supernatant liquid was collected in a preweighed aluminum dish (W 2 ) and dried at 105 • C for 24 h. The weight of the dried residue (W 3 ) was measured. The water solubility index (WSI) was expressed by the following Equation (3): (3)

Sensorial Evaluation Using a Descriptive Analysis
A descriptive analysis method was applied to evaluate the sensory properties of mushroom powder [19,20]. For the sensory evaluation, powder samples (5 g) were placed in white dishes. The dishes were labeled with three-digit random numbers. Twelve panelists were selected from the graduate students of Chung-Ang University. Descriptors and definitions used for the sensory evaluation of vitamin D 2 fortified mushroom powder are shown in Table 2. Three sensory attributes (roughness, mushroom aroma and mushroom taste) were evaluated to determine the sensorial property of mushroom powder.

Statistical Analysis of Data
All experiments were performed in triplicate. The results were expressed as the mean ± standard deviation. An analysis of variance (ANOVA) and Duncan's multiple range comparison tests were performed using SPSS Statistics 19.0 software (IBM, New York, NY, USA), with p-values < 0.05 considered significant. Figure 1 shows the appearance of UV-B-irradiated mushroom samples. Figure 1A shows the UV-B-irradiated mushrooms after various irradiation times, and Figure 1B shows the UV-B-irradiated mushroom samples after freeze-drying. There was a visible change in the appearance of the surface of the mushroom slices; the surface color changed from white to brown. A previous study indicated that UV-C irradiation also led to mushroom discoloration [21]. Long-term UV-B irradiation may result in degraded mushroom quality due to surface discoloration. The surface discoloration of mushrooms affects the acceptability of the product. The irradiated samples were freeze-dried, and the surface discoloration of the UV-B-treated mushrooms remained after freeze-drying ( Figure 1B). Foods 2020, 9, x FOR PEER REVIEW 5 of 11

Appearance of UV-B-Irradiated Mushrooms
indicated that UV-C irradiation also led to mushroom discoloration [21]. Long-term UV-B irradiation may result in degraded mushroom quality due to surface discoloration. The surface discoloration of mushrooms affects the acceptability of the product. The irradiated samples were freeze-dried, and the surface discoloration of the UV-B-treated mushrooms remained after freeze-drying ( Figure 1B).

Effect of UV-B Irradiation on Vitamin D2 and Ergosterol Concentrations of Mushrooms
The effect of UV-B irradiation on the vitamin D2 and ergosterol concentrations of white button mushrooms is shown in Figure 2. The vitamin D2 concentration in the untreated control was 0.02 μg/g ( Figure 2A). After exposure to UV-B for 10, 20 and 30 min, the vitamin D2 concentration increased to 4.93, 6.50 and 8.19 μg/g, respectively. These observations indicated that the conversion of ergosterol into vitamin D2 was dependent on the UV-B irradiation time in the early time points. However, UV-B irradiation times longer than 30 min did not further increase vitamin D2 concentration. After 30, 60 and 90 min of UV-B irradiation, the vitamin D2 concentrations were statistically identical (p < 0.05). Overall, the vitamin D2 concentration of mushrooms irradiated with UV-B for 30 min was about 400 times greater than that of the control and remained constant following further UV-B treatment. Therefore, the optimal UV-B irradiation time for the white button mushrooms was determined to be 30 min.

Effect of UV-B Irradiation on Vitamin D 2 and Ergosterol Concentrations of Mushrooms
The effect of UV-B irradiation on the vitamin D 2 and ergosterol concentrations of white button mushrooms is shown in Figure 2. The vitamin D 2 concentration in the untreated control was 0.02 µg/g (Figure 2A). After exposure to UV-B for 10, 20 and 30 min, the vitamin D 2 concentration increased to 4.93, 6.50 and 8.19 µg/g, respectively. These observations indicated that the conversion of ergosterol into vitamin D 2 was dependent on the UV-B irradiation time in the early time points. However, UV-B irradiation times longer than 30 min did not further increase vitamin D 2 concentration. After 30, 60 and 90 min of UV-B irradiation, the vitamin D 2 concentrations were statistically identical (p < 0.05). Overall, the vitamin D 2 concentration of mushrooms irradiated with UV-B for 30 min was about 400 times greater than that of the control and remained constant following further UV-B treatment. Therefore, the optimal UV-B irradiation time for the white button mushrooms was determined to be 30 min.
It has been suggested that only a small fraction of ergosterol is converted to vitamin D 2 by UV irradiation [18]. The conversion of ergosterol to vitamin D 2 in the early stage of the process is a typical zero-order reaction where the concentration of the reacting compound, i.e., ergosterol, is so large that the overall reaction rate appears to be independent of its concentration [22]. Our results support this explanation because there was little difference in the ergosterol concentration after UV-B irradiation ( Figure 2B). The amount of ergosterol within the mushrooms was estimated to be a hundred-fold higher than that of vitamin D 2 . It has been suggested that only a small fraction of ergosterol is converted to vitamin D2 by UV irradiation [18]. The conversion of ergosterol to vitamin D2 in the early stage of the process is a typical zero-order reaction where the concentration of the reacting compound, i.e., ergosterol, is so large that the overall reaction rate appears to be independent of its concentration [22]. Our results support this explanation because there was little difference in the ergosterol concentration after UV-B irradiation ( Figure 2B). The amount of ergosterol within the mushrooms was estimated to be a hundred-fold higher than that of vitamin D2.

Size Distributions of Coarse, Fine and Superfine Mushroom Powders
The UV-B-treated mushrooms were freeze-dried and pulverized into coarse, fine and superfine powders. The particle size distributions of the mushroom powders are shown in Table 3. The results are presented as the equivalent diameter at a cumulative volume of 10% (Dv10), 50% (Dv50) and 90% (Dv90). The Dv50 values of the coarse, fine and superfine powders were 231.0 ± 1.7, 106.3 ± 2.1 and 7.1 ± 0.3 μm, respectively. The specific surface area of the mushroom powders increased as the particle size decreased. The results indicated that jet-milling effectively pulverized the mushroom into a superfine scale of <10 mm and confirmed the homogeneous size distribution of superfine powder (Figure 3).

Size Distributions of Coarse, Fine and Superfine Mushroom Powders
The UV-B-treated mushrooms were freeze-dried and pulverized into coarse, fine and superfine powders. The particle size distributions of the mushroom powders are shown in Table 3. The results are presented as the equivalent diameter at a cumulative volume of 10% (Dv 10 ), 50% (Dv 50 ) and 90% (Dv 90 ). The Dv 50 values of the coarse, fine and superfine powders were 231.0 ± 1.7, 106.3 ± 2.1 and 7.1 ± 0.3 µm, respectively. The specific surface area of the mushroom powders increased as the particle size decreased. The results indicated that jet-milling effectively pulverized the mushroom into a superfine scale of <10 mm and confirmed the homogeneous size distribution of superfine powder (Figure 3). Table 3. Particle size and specific surface area of coarse, fine and superfine powders produced from white button mushrooms exposed to a 30-min UV-B treatment. All values are expressed as the mean ± standard deviation of triplicate analyses. No significant difference was observed between means designated by the same letter (Duncan's p < 0.05).

Figure 3.
Particle size distribution of coarse, fine and superfine mushroom powders. The white button mushrooms were UV-B treated for 30 min and freeze-dried before pulverization.
The reduced particle size of the superfine mushroom powder is beneficial for its use as a food ingredient because it minimizes the rough mouthfeel and fibrous texture. The application of jet milling successively can reduce the particle size of fibrous material below 10 μm, which has been reported for soybean flour [11], the insoluble fiber-rich fraction of carrot [23] and mushroom stem and cap powders [14].

Microstructure of Coarse, Fine and Superfine Mushroom Powders
The microstructure of mushroom powders was observed using SEM ( Figure 4). The coarse and fine mushroom powders that were prepared by a conventional mixer followed by sifting ( Figure  4A,B) had a flatter, flakier and more irregular shape because the mushrooms were mainly composed of fibers and carbohydrates [13]. On the other hand, the particle size of jet-milled, superfine mushroom powder ( Figure 4C) was significantly smaller than that of the other samples. The superfine mushroom powder had a less flaky shape and was more homogenous in size. Homogeneity is a desirable property for food ingredients, and the jet milling process imparts a physical functionality to mushroom powder. The reduced particle size of the superfine mushroom powder is beneficial for its use as a food ingredient because it minimizes the rough mouthfeel and fibrous texture. The application of jet milling successively can reduce the particle size of fibrous material below 10 µm, which has been reported for soybean flour [11], the insoluble fiber-rich fraction of carrot [23] and mushroom stem and cap powders [14].

Microstructure of Coarse, Fine and Superfine Mushroom Powders
The microstructure of mushroom powders was observed using SEM ( Figure 4). The coarse and fine mushroom powders that were prepared by a conventional mixer followed by sifting ( Figure 4A,B) had a flatter, flakier and more irregular shape because the mushrooms were mainly composed of fibers and carbohydrates [13]. On the other hand, the particle size of jet-milled, superfine mushroom powder ( Figure 4C) was significantly smaller than that of the other samples. The superfine mushroom powder had a less flaky shape and was more homogenous in size. Homogeneity is a desirable property for food ingredients, and the jet milling process imparts a physical functionality to mushroom powder.

Vitamin D2 Concentrations of Coarse, Fine and Superfine Mushroom Powders
The vitamin D2 concentrations of the coarse, fine and superfine mushroom powders were 7.6 ± 0.3, 7.8 ± 0.5 and 8.1 ± 0.1 μg/g, respectively ( Figure 5). The vitamin D2 concentration of unpulverized mushrooms was 8.2 ± 0.3 μg/g. There was no statistical difference among the samples. The results showed that there was no loss of vitamin D2 due to the grinding of the mushrooms, including the jet milling process.

Vitamin D 2 Concentrations of Coarse, Fine and Superfine Mushroom Powders
The vitamin D 2 concentrations of the coarse, fine and superfine mushroom powders were 7.6 ± 0.3, 7.8 ± 0.5 and 8.1 ± 0.1 µg/g, respectively ( Figure 5). The vitamin D 2 concentration of unpulverized Foods 2020, 9, 1713 8 of 11 mushrooms was 8.2 ± 0.3 µg/g. There was no statistical difference among the samples. The results showed that there was no loss of vitamin D 2 due to the grinding of the mushrooms, including the jet milling process.
at 300× magnification. The white button mushrooms were UV-B treated for 30 min and freeze-dried before pulverization.

Vitamin D2 Concentrations of Coarse, Fine and Superfine Mushroom Powders
The vitamin D2 concentrations of the coarse, fine and superfine mushroom powders were 7.6 ± 0.3, 7.8 ± 0.5 and 8.1 ± 0.1 μg/g, respectively ( Figure 5). The vitamin D2 concentration of unpulverized mushrooms was 8.2 ± 0.3 μg/g. There was no statistical difference among the samples. The results showed that there was no loss of vitamin D2 due to the grinding of the mushrooms, including the jet milling process. Figure 5. Effect of particle size reduction on the vitamin D2 concentration of mushroom powders. The control mushrooms were UV-B irradiated (30 min) and freeze-dried. Coarse fine and superfine mushroom powders were prepared using the control mushrooms. All values were expressed as the mean ± standard deviation of triplicate analysis. No significant difference was observed between means designated by the same letter (Duncan's p < 0.05).
According to a previous study, vitamin D2 from irradiated white button mushrooms is strongly absorbed and metabolized, as demonstrated by the 25-hydroxyvitamin D serum response to rats fed with the irradiated mushrooms [21]. It has been predicted that the amplified vitamin D2 within mushroom powders could be efficiently absorbed and metabolized. Table 4 shows the hydration properties of the mushroom powders with different particle sizes. The water holding capacity decreased significantly with decreasing particle size (p < 0.05). The effect of reduced particle size on the water holding capacity of a powder remains controversial. The water holding capacity in superfine silver carp bone powder was found to be higher than in coarser powders [24], whereas the superfine grinding of wheat bran resulted in a reduced water holding The control mushrooms were UV-B irradiated (30 min) and freeze-dried. Coarse fine and superfine mushroom powders were prepared using the control mushrooms. All values were expressed as the mean ± standard deviation of triplicate analysis. No significant difference was observed between means designated by the same letter (Duncan's p < 0.05).

Hydration Properties of the Coarse, Fine and Superfine Mushroom Powders
According to a previous study, vitamin D 2 from irradiated white button mushrooms is strongly absorbed and metabolized, as demonstrated by the 25-hydroxyvitamin D serum response to rats fed with the irradiated mushrooms [21]. It has been predicted that the amplified vitamin D 2 within mushroom powders could be efficiently absorbed and metabolized. Table 4 shows the hydration properties of the mushroom powders with different particle sizes. The water holding capacity decreased significantly with decreasing particle size (p < 0.05). The effect of reduced particle size on the water holding capacity of a powder remains controversial. The water holding capacity in superfine silver carp bone powder was found to be higher than in coarser powders [24], whereas the superfine grinding of wheat bran resulted in a reduced water holding capacity [25]. Our previous studies also produced contradictory results. The jet milling of defatted soybean powder resulted in a significant increase in water holding capacity [11], whereas the same procedure resulted in a significant decrease in the water holding capacity of Hericium erinaceum [13]. It is likely that jet milling induces an increase in water holding capacity by increasing the surface area; however, at the same time, it also induces a decrease in the water holding capacity by breaking down the complexity of the microstructure. Our current results indicate that the breakdown of complexity is a more important factor than the increase in the surface area following the jet milling of mushroom powder, which results in a reduced water holding capacity.

Hydration Properties of the Coarse, Fine and Superfine Mushroom Powders
The swelling capacity indicates how well the sample expands by absorbing water. The results indicate that coarse mushroom powders absorb more water and swell more than fine mushroom powders. Table 4. Hydration properties of coarse, fine and superfine mushroom powders produced from white button mushrooms exposed to a 30 min UV-B treatment.

Sample
Water Holding Capacity (g) All values are expressed as the mean ± standard deviation of triplicate analyses. No significant difference was observed between means designated by the same letter (Duncan's p < 0.05).
The water solubility index represents how well a sample is dissolved in water. The water solubility index of mushroom powder increased with decreasing particle size, with superfine mushroom powder having the largest water solubility index value of 54.19 ± 1.08%. According to a previous study, the water solubility index increases with decreasing particle size, which is due to the increased surface area and improved solubilization of protein [24]. This means that superfine mushroom powder is more soluble in water than coarser powders and would therefore be easier to use as a food ingredient in processed food.

Sensory Evaluation of the Coarse, Fine and Superfine Mushroom Powders
The sensory properties of the mushroom powders were analyzed by a descriptive analysis method (Table 5). Three sensory attributes (roughness, mushroom aroma and mushroom taste) were assessed alongside previously identified standards, as shown in Table 2. Table 5. Sensory properties of coarse, fine and superfine mushroom powders produced from white button mushrooms exposed to a 30 min UV-B treatment. All values are expressed as the mean ± standard deviation of triplicate analyses. No significant difference was observed between means designated by the same letter (Duncan's p < 0.05).

Sample
Roughness was defined as the mouthfeel of the particles. As expected, roughness decreased significantly as the particle size of mushroom powder decreased (p < 0.05).
The aroma and taste of mushrooms also decreased significantly as the particle size of the mushroom powder decreased. The reduction in mushroom aroma and taste in superfine mushroom powder was unexpected because the increased surface area of superfine powder was expected to possibly accelerate the leaching of aroma and taste compounds. One possible explanation for this unexpected observation is that the compounds responsible for the mushroom aroma and taste were partly removed during the size reduction and jet milling processes. The acceptability of a mushroom's aroma and the taste is strongly dependent on personal preference. These results show that superfine mushroom powder can be a good natural source of vitamin D 2 for consumers who dislike mushrooms because it exhibits a weaker mushroom aroma and taste than other powders. The results indicated that the superfine mushroom powder with vitamin D 2 could be applied to processed foods such as beverages, soups or and confectionaries, where the sensorial properties determine the acceptability of products.

Conclusions
The nutritional and physical properties of the white button mushroom were tailored through the consecutive physical application of UV-B irradiation and jet milling. Exposure to UV-B effectively increased the vitamin D 2 concentration of mushrooms by up to 400 times after 30 min of irradiation.
Vitamin D 2 -fortified mushrooms were then sized into coarse (Dv 50 = 231.0 µm), fine (Dv 50 = 106.3 µm) and superfine (Dv 50 = 7.1 µm) powders. There were no significant changes in the vitamin D 2 concentration following the grinding process. Through a descriptive analysis, the superfine powder was confirmed to have reduced roughness and weaker mushroom aroma and taste compared to the other samples. These results indicate that the UV-B-treated superfine mushroom powder can be a good natural source of vitamin D 2 for consumers who dislike mushrooms because it exhibits a weaker mushroom aroma and taste than other powders.