The Complexity of Chinese Cereal Vinegar Flavor: A Compositional and Sensory Perspective

With a millennium-long history, traditional Chinese cereal vinegar (CCV) is a significant part of China’s cultural heritage. The unique flavor of CCV is derived from the use of cereal and its bran as raw materials and solid-state fermentation as a brewing technique. This paper systemically summarized recent research progress on the aroma compounds in CCV, the biochemical generation of aroma compounds during the brewing process, and the association between sensory perception and the primary aroma compounds. Furthermore, a complete CCV lexicon and sensory wheel prototype were constructed. This study aims to lay a foundation for future CCV aroma research, quality improvement, and industrialization.


Introduction
Traditional Chinese cereal vinegar (CCV) dates to the Zhou dynasty (1027-221 BCE) and has existed for thousands of years, with the first professional vinegar production workshop established in the Chunqiu Dynasty (770-476 BC) [1].The classic solid-state fermentation technique used for traditional CCV has been perfected over thousands of years, propelled by intricate, natural microbial communities.Over 20 CCV types are commercially available in China.
The four most popular CCVs in China include Shanxi aged vinegar (SAV) (using a sorghum raw material and a Daqu starter), Zhenjiang aromatic vinegar (ZAV) (using sticky rice and a Daqu starter), Sichuan bran vinegar (SBV) (using wheat bran, various spices and herbs, and Daqu), and Yongchun Monascus vinegar (YMV) (using red yeast for saccharification, sticky rice, and liquid-state fermentation) [2].Additionally, other CCVs, such as Beijing rice vinegar (BRV), Zhengrong rice vinegar (ZRV), and Zhejiang rose vinegar, are also popular.Unlike vinegars in other part of world, like Italian balsamic vinegar produced from grapes [3], Middle East vinegar from dates [4], and Philippines vinegar from coconut palm and sugar cane [5], the unique flavor of CCV can be attributed to the fermentation technique and raw materials used during production, including buckwheat, sorghum, corn, wheat, barley, and rice.CCVs are produced via solid-state fermentation, involving microbial proliferation on solid, moist substrates in the absence of free-flowing water, differing from the liquid fermentation technique used for Italian balsamic vinegar and sherry vinegar or slow traditional acetification processes (the Orléans or French method) for wine vinegar [6].
Foods 2024, 13, 756 2 of 17 However, maintaining flavor quality, stability, and consistency between different CCV batches during traditional fermentation in open conditions is challenging due to a lack of systematic aroma compound assessment.Since aroma compounds crucially impact vinegar quality and sensory acceptability, their identification and quantitation in related products have attracted significant research attention over the past 10 years.The rapid advances in analytical methods and molecular sensory science have gradually started to reveal the chemical nature of CCV flavor and its formation mechanism.This study aims to promote the understanding of CCV aroma profiles, providing a theoretical foundation for qualitative and quantitative aroma compound research and quality control.

Types of CCV 2.1. Shanxi Aged Vinegar (SAV)
SAV, originating in the northern Chinese province of Shanxi, has been registered as a geographical indication (GI) product of China, forming part of its cultural heritage.Cultivated in the north of China, sorghum is the main ingredient in SAV, while other raw materials include rice hulls, millet bran, wheat bran, and peas.SAV is produced via solidstate fermentation in an open-style environment using various fungal and bacterial species.Traditional SAV fermentation involves several steps, including the preparation of the Daqu, saccharification of the starch, fermentation of the alcohol and acetic acids, thermal processing, and aging (Figure 1) [7,8].Daqu is produced via spontaneous microorganism growth on peas and barley, accounting for approximately 60% of the raw material.The raw ingredients are fermented underground in a large jar for about 13 d, after which porosity is increased by mixing wheat bran and rice hulls with the alcoholic samples (Jiupei in Chinese) to enhance heat discharge and oxygen absorption.At the beginning of the 7 d acetic acid fermentation period, previous sample batch vinegar seeds (Cupei) are added to the jar.During the one-week thermal process, the Cupei is transferred to a jar, placed on a stove, and roasted.The aging process commonly lasts three to five years, involving water evaporation in the summer or freezing and collection in the winter.Although the SAV flavor, obtained via thermal processing, is typically sour and vinegary, it is also soft, fragrant, and mild.

Zhenjiang Aromatic Vinegar (ZAV)
ZAV, also known as Zhenjiang Xiang Cu in Chinese, was registered as both a GI product of China in 2001 and PGI of the EU in 2012 according to regulation (EU) No. 501/2012.ZAV has EU protection status under the PGI and PDO program, along with three types of balsamic vinegar products from Italy.ZAV is primarily manufactured using wheat bran, Daqu (consists of bacteria-, mold-, and yeast-containing fermented cereal), and sticky rice (mainly cultivated in southern China).Figure 1 shows a flowchart of the production process, which involves alcoholic liquid-state and acetic acid solid-state fermentation processes, leaching, decoction (liquid vinegar boiling), and aging.Acetic acid is fermented using either a traditional or industrial technique [9].The traditional method is also known as the surface or slow technique since the acetic acid bacterial starter grows on the Cupei surface in a pottery jar and requires about two months of fermentation at 25 °C.The industrial, or modern, method involves submersion, where mechanical agitation is employed to provide the added acetic acid bacteria with the required oxygen to accelerate fermentation.This is a quick fermentation process that occurs in an oblong cement pool for about 20 d, involving lactic and acetic acid bacteria, mold, and yeast, to produce various metabolites.Furthermore, decoction and extended aging periods (several months or longer) may induce complex chemical reactions, such as esterification, amino acid and saccharide hydrolysis, and the Maillard reaction, in weakly acidic conditions, increasing the compositional complexity and exceptional flavor of ZAV.

Zhenjiang Aromatic Vinegar (ZAV)
ZAV, also known as Zhenjiang Xiang Cu in Chinese, was registered as both a GI product of China in 2001 and PGI of the EU in 2012 according to regulation (EU) No. 501/2012.ZAV has EU protection status under the PGI and PDO program, along with three types of balsamic vinegar products from Italy.ZAV is primarily manufactured using wheat bran, Daqu (consists of bacteria-, mold-, and yeast-containing fermented cereal), and sticky rice (mainly cultivated in southern China).Figure 1 shows a flowchart of the production process, which involves alcoholic liquid-state and acetic acid solid-state fermentation processes, leaching, decoction (liquid vinegar boiling), and aging.Acetic acid is fermented using either a traditional or industrial technique [9].The traditional method is also known as the surface or slow technique since the acetic acid bacterial starter grows on the Cupei surface in a pottery jar and requires about two months of fermentation at 25 • C. The industrial, or modern, method involves submersion, where mechanical agitation is employed to provide the added acetic acid bacteria with the required oxygen to accelerate fermentation.This is a quick fermentation process that occurs in an oblong cement pool for about 20 d, involving lactic and acetic acid bacteria, mold, and yeast, to produce various metabolites.Furthermore, decoction and extended aging periods (several months or longer) may induce complex chemical reactions, such as esterification, amino acid and saccharide hydrolysis, and the Maillard reaction, in weakly acidic conditions, increasing the compositional complexity and exceptional flavor of ZAV.

Sichuan Bran Vinegar (SBV)
Sichuan Baoning vinegar, an SBV mainly produced in Baoning town, Sichuan province, primarily uses a wheat bran raw material, a Daqu saccharification starter, and various Chinese herbs.SBV is considered the sole medicinal Chinese vinegar due to the herbs used, such as Villous amomum fruit, Eucommia bark, and licorice, which promote fungal proliferation (i.e., Rhizopus spp.) in the Daqu during fermentation and contribute to the unique aroma profile [10,11].The traditional brewing process includes Daqu production and fermentation.Solid-state SBV fermentation is a spontaneous, open process fueled by reproducible microbiota involving simultaneous acetic acid and alcohol fermentation and saccharification [12].Similar to ZAV, this process can use both traditional and modern techniques [10,12].Fermentation is followed by the aging process for two to three years in a pottery jar.During the day, the sealed vinegar is exposed to the sun while absorbing dew at night. Figure 1 shows the specific SBV production process.

Yongchun Monascus Vinegar (YMV)
With a history of over 2000 years, YMV presents a unique flavor and bioactivity.YMV is recognized as a GI product produced in Yongchun County, Fujian Province.Unlike other traditional CCVs, YMV is produced via liquid fermentation using a glutinous rice raw material and a Hongqu fermentation starter, i.e., using glutinous rice to culture Monascus spp.[13].YMV is placed in a pot or jar for an extended period to allow the development of the taste, flavor, and beneficial nutritional properties.

Other CCV
Longmen rice vinegar, a famous brand of BRV, has a consumption history in the Beijing region of approximately 200 years.The BRV production process differs from other CCVs since fermentation directly follows rice grinding without thermal treatment (Figure 1) [2].The raw materials of other CCVs are typically pretreated with steam.At the beginning of fermentation, the Daqu and yeast are incubated in a tank to promote alcohol formation.During acetic acid fermentation, rice husk and bran, as well as Cupei obtained from the previous batch of acetic acid fermentation, are introduced into the tank.The brewing process enters the aging phase, during which the flavor is enhanced.
Traditional Chinese rose vinegar, which is highly popular in southern China due to its distinct color and flavor, is fermented via natural microorganisms falling into the fermentation container.The fermentation process consists of three stages: sticky rice starch degradation into sugars by mold, sugar conversion to ethanol by yeast, and acetic acid formation from ethanol via bacteria.Chinese rose vinegar tends to vary in different seasons since its quality is highly dependent on environmental factors due to the natural production process.

The Research Advancements on the CCV Flavor Components
Over the past 15 years, many qualitative and quantitative studies have investigated CCV aroma compounds by employing gas chromatography-mass spectrometry (GC-MS), twodimensional gas chromatography-time-of-flight mass spectrometry (GC×GC-TOF-MS), gas chromatography-ion mobility spectrometry (GC-IMS), chromatography-olfactometry-mass spectrometry (GC-O-MS), and gas chromatography-olfactometry (GC-O).Advances in analytical methods have increased the number of identified CCV flavor components.Table 1 only lists some aroma-active components, given the significant number of identified volatile organic compounds.
In 2019, Al-Dalali et al. used SPME-GS-MS and GC-O to compare traditional ZAV aroma profiles with modern ZAV during aging [9], identifying 53 volatile compounds, of which 43 were positively determined based on comparison with standard compounds.Furthermore, aroma compound differences were evident between the traditional and modern ZAV during aging.For example, isopentyl alcohol, 2-acetoxy-3-butanone, and 3-(methylthio)propyl acetate were present in the traditional ZAV, while the modern vinegar contained octanoic acid, dimethyl trisulfide, propiophenone, and 2-ethyl-1-hexanol.The total number of volatile substances was higher in the modern ZAV than in the traditional samples.In 2020, Zhou et al. characterized ZAV aroma compounds using omission experiments, aroma recombination, GC-O-MS, and OAVs [20].Sensory assessment showed that aged ZAV displayed a more intense buttery caramel odor and higher complexity than fresh ZAV.Here, 68 compounds were identified and evaluated, with the presence of sotolon detected for the first time in CCV.OAV calculation identified 27 odorants as crucial aged ZAV aroma compounds.Aroma recombination could effectively simulate the aged vinegar aroma profile, while omission experiments verified the role of the primary aroma compounds, including acetic acid, tetramethylpyrazine, 3-methylbutanoic acid, 2,4,5-trimethyloxazole, sotolon, 2-methylpropanal, and 2,3-butanedione.

Sichuan Bran Vinegar (SBV)
In 2020, Al-Dalali et al. analyzed the key aroma substances and profiles in modern and traditional SBV via GC-O and SAFE-GC-MS [10].They tentatively detected 99 volatile substances, of which 77 were then positively determined via comparison with standard com-pounds.Then, an aroma extract dilution assay (AEDA) was combined with GC-O to characterize 42 aroma-active compounds with FD factors (1 to 6561).Ten of these compounds were detected for the first time in CCV, while OAV calculation showed that 26 were key aroma compounds, with 3-oxobutan-2-yl acetate, acetic acid, furan-2-carbaldehyde, butyrolactone, and 2-hydroxy-3-butanone exhibiting the highest levels in both the traditional and modern SBV.Solution reconstitution showed that the SBV aroma profile could be closely simulated via aroma recombination in terms of fruitiness, roasted aroma, sweetness, woodiness, and spiciness while displaying slight differences in the herbal and nutty notes.

Other CCV
In 2019, Al-Dalali et al. characterized Zhengrong rice vinegar (ZRV) and Longmen smoked vinegar (LSV) aroma profiles using SPME-GC-MS and GC-O [15], identifying 75 volatile compounds, of which 42 were confirmed via authentic substances.Higher ester and phenol levels were found in the ZRV, while the LSV contained more ketones, aldehydes, and pyrazines.In 2020, the same research team studied the aroma compounds of three kinds of Longmen rice vinegar using SPME-GC-MS and AEDA-GC-O [22], identifying 68 volatile compounds, of which 49 were verified via chemical standards.Higher aromatic hydrocarbon, acetal, sulfides, ester, alcohol, and acid concentrations were evident in the aromatic sweet rice vinegar sample due to the presence of additional sugar and solarization.The aromatic rice vinegar sample contained higher oxazole, lactone, phenol, ketone, pyrazine, and aldehyde levels, which could be attributed to added spices and solarization.In 2020, Zhao et al. examined the aroma profile of traditional Chinese rose vinegar using GC-MS-O and SPME-GC-MS [23], detecting 48 flavors and comparatively high acid and aldehyde concentrations.Furthermore, OAV calculation showed that aldehydes, such as dodecanal, heptanal, decanal, 3-methyl-butanal, and nonanal, likely contributed significantly to the aroma of this vinegar.

The Biochemical Generation of Key Aromas during the Brewing Process
Aroma compound variation was evident during different CCV fermentation stages.An abundance of heterocyclic compounds, alcohols, aldehydes, and esters was evident during the fermentation process, while thermal processing yielded ketones, aldehydes, pyrazines, and various other aroma substances, which were related to chemical processes like the Maillard reaction and hydrolysis.The aging process involves water evaporation, as well as chemical changes and reactions.The biochemical generation of the primary CCV aroma compounds are described in the following sections.

Fermentation
Significant heterocyclic compound, alcohol, ketone, aldehyde, and ester formation was evident during the SAV fermentation process.Nie et al. investigated the microbiota succession in traditional SAV during fermentation, indicating dramatic bacterial community compositional changes at different fermentation phases.The number of bacterial genera in the Daqu (relative abundance >0.1%) decreased from 17 to 2 at 12 d of alcohol fermentation.The 15 genera present at 1 d of the acetic acid fermentation decreased to 4 at 7 d, which included Acetobacter (50.9%),Lactobacillus (47.9%),Komagataeibacter (0.7%), and Propionibacterium (0.1%).The fungal community structure displayed more homogeneity, with Saccharomycopsis and Saccharomyces dominating during alcohol and acetic acid fermentation [25].Re-inoculating the indigenous P. manshurica yeast strain into the Daqubased fermentation increased the total ester, acetic acid, and ethanol levels in the SAV, producing a pleasant floral, fruity flavor and improving the aftertaste [26].The activity of microorganisms can produce 3-hydroxy-2-butanone and 2,3-butanedione during the alcoholic fermentation of food products and beverages, such as wine.
Huang et al. demonstrated the effect of various starters on ZAV fermentation [27].Acetic acid fermentation represented the primary phase for flavor compound formation during the brewing process.The acid content, including citric, lactic, and acetic acids, and volatile compounds, such as 2,3-butanedione and acetoin, continued to increase during the sealed fermentation phase.The bacterial and fungal community structures varied in the different fermentation stages.The dominant bacterial operational taxonomic units with average relative abundance values exceeding 10% in at least one fermentation phase included Pseudomonas, Lactobacillus, Aeromonas, Acinetobacter, Acetobacter, and Acetilactobacillus, while the most abundant fungal populations in each fermentation phase displayed obvious divergence, and included Fusarium, Alternaria, Saccharomyces, and Wickerhamomyces.

The Thermal and Decoction Processes
The thermal and decoction processes are typical steps during the production of SAV and ZAV, respectively, providing the distinctive burnt, caramel-like aromas of these vinegars.Two important chemical reactions occur during thermal and decoction processing, namely hydrolysis and the Maillard reaction.First, the residual microorganism metabolites, hemicellulose, protein, and starch are gradually hydrolyzed to reducing sugars and amino acids in weakly acidic conditions, which are then transformed into aroma compounds via the Maillard reaction, including pyrazines, aldehydes, and ketones.
Pyrazines represent characteristic CCV aroma compounds, which are rarely present in European vinegar, like red wine or sherry vinegar.Pyrazines are commonly found in roasted food and beverages, such as coffee, contributing to the desirable roasted flavor [28,29].They are likely products of the Maillard reaction via Strecker degradation.The SAV contained 2,3-dimethylpyrazine, tetramethylpyrazine, and trimethylpyrazine [8].Tetramethylpyrazine was selected as a quality marker for SAV according to the "Product of geographical indication-Shanxi extra aged vinegar" Chinese national standard.Here, 19 pyrazines, mostly saturated alkyl pyrazines, were detected in the ZAV [16].The Maillard reaction is likely responsible for oxazole production during heat treatment in the presence of high amino acid and sugar levels.
Aldehydes, especially furfural and its derivatives, substantially impacted the ultimate sensory perception of CCV, contributing nutty, roasted, and almond flavors.Furfural presented roasted and caramel aromas resulting from Strecker degradation at high temperatures [30].The Maillard reaction also facilitated the formation of the potent aroma compound 5-Methylfurfural, while a considerable number of furans were detected in the ZAV [16].

Aging Process
Aging is crucial for enhancing the unique flavor of CCV.During SAV aging, the water content continued to decrease via evaporation and ice formation in the open jar, while the volatile levels also tended to decline.In addition to physical changes, various complex chemical reactions occurred.Liang et al. compared the aroma substances in SAV prior to and following the aging process [14], showing that the active odor compounds were mostly the same, while their quantities differed.The increase in aroma substances like dimethyl trisulfide, phenylacetaldehyde, and tetramethylpyrazine and the presence of pyrazine compounds indicated that the Maillard reaction continued during aging.This was reasonable since the SAV was in reducing sugars, amino acids, and proteins.The change in the traditional ZAV aroma profile during aging was also investigated [9].The results showed that the total aldehyde, ester, alcohol, ketone, furan, and pyrazine concentrations decreased after aging, possibly due to chemical evaporation.Contrarily, the content of some volatile compounds increased, including 2-acetoxy-3-butanone, 2-phenethyl acetate, ethyl benzene acetate, ethyl acetate, and furfural.

The Correlation between Sensory Perception and Aroma Substances
OAV calculation and AEDA are two widely used techniques for establishing a connection between aroma compounds and sensory quality analytical data.OAV is used to link the quantitative information obtained via chemical analysis to sensory perception, providing an effective method to compare sample aroma profiles [31].In terms of AEDA, the FD factor of a compound can no longer be regarded as its maximum dilution time [14].A higher FD factor indicates a more significant compound contribution to the overall aroma perception.

The Sensory Perception of CCV 6.1. Lexicon
A shared lexicon is essential to effectively analyze the sensory perception of food.Lexicons are organized, established, and standardized vocabulary lists that can be used to accurately evaluate the sensory qualities of food or beverages, enhancing communication between researchers, product developers, and business partners.Lexicons are currently available for wine [32], beer [33], green tea [34], and more recently, coffee [35] and spices [36].
A lexicon differs significantly from other sensory evaluation tools in three key ways.The first involves description.A lexicon is a solely descriptive tool and does not allow for ranking quality, nor does it have categories for "good" and "bad" features.The second involves quantification.For instance, using a lexicon enables researchers to specify that a product has a blueberry flavor or an aroma intensity of 4 on a 15-point scoring scale, in addition to merely stating that it has a blueberry flavor or aroma.This significantly increases the accuracy of CCV difference comparisons.The third involves replicability.If trained sensory professionals utilize a lexicon properly, the same CCV assessed by two different individuals will yield the same intensity score for each attribute, regardless of their location, past taste experiences, cultural background, or any other distinction.For example, evaluators in Shanxi and Jiangsu will both perceive "blueberry, flavor: 4."Moreover, a sensory lexicon is a dynamic resource that will be revised over time to incorporate new characteristics and references.
A lexicon with accurate definitions and references that can consistently distinguish and describe the sensory attributes will be helpful to producers, salespeople, researchers, and consumers, among other stakeholders in the CCV value chain.This study reviewed all the possible descriptors for the aroma and flavor of CCVs, irrespective of the area of origin, type, and raw material (Table 2).Trained panels of tasters developed a lexicon for describing CCVs, which defined and referenced 23 significant olfactory descriptors, namely chocolate, toasted, meat broth, licorice, walnut, yogurt, fruity, glue, honey, leather, caramel, burnt, flour, vanilla, spicy, smoked, green, bitter almond, mildew, rancid, boiled vegetable, butter, and woody flavors [37].However, the judges in this study were all of Italian descent and were not very familiar with CCV.Another Chinese study selected ten olfactory terms for CCV aroma, namely sour, sweet, burnt, sauced, fruity, bran, incense, incense smoke, grain, alcoholic, and flowery [38].A comprehensive, standard, and dynamic lexicon is necessary for characterizing the aroma and flavor of CCVs in both English and Chinese.

Sensory Wheel
The sensory wheel is a useful visual aid for characterizing the flavor attributes of food products.It works by gathering, categorizing, arranging, and summarizing particular sensory attribute descriptors to represent the flavor characteristics of tested samples.The sensory wheel was created using lexicons and the findings of a professional sensory panel via a sensory evaluation [36].Sensory wheels are more visual compared to lexicons.The sensory wheel helps producers better manage the quality of their products and provides the groundwork for future research and flavor discovery, which aids in both product development and improvement.The flavor wheel is currently employed in the tea [39], coffee [35], wine [40], and chocolate [41] industries, as well as various other fields.
Multivariate statistical methods are used to filter for precise and useful sensory descriptors.Next, to provide a summary, the descriptors are split into two or three levels.First-level terms fall under the macro category and are typically categorized based on how they are recognized (e.g., aroma, flavor, mouthfeel, and texture).First-level terms are used to refine and categorize second-level terms.Concrete object descriptors are referred to by the third-level terms.Finally, an image of a circular wheel represents the three levels of descriptors [42].Multivariate statistical methods were utilized in the construction of the sensory wheel.Agglomerative hierarchical clustering (AHC), for instance, was utilized to identify the primary categories, subcategories, and tiers.Additionally, multidimensional scaling (MDS) was utilized to ascertain how these categories and subcategories were arranged within the wheel structure [35].
An initial version of the CCV sensory wheel was first built by Kong et al., who included a total of 45 sensory descriptors in 16 categories (Figure 2) [38].The CCV sensory wheel is divided into several sections to help consumers visualize the different flavors, scents, and aromatic qualities found in most CCV products, including SAV, ZAV, and SBV.However, compared to other food products and beverages, like wine and coffee, more systematic research is required to construct a practical, accurate CCV sensory wheel.Innovative statistical and sensory techniques are necessary for constructing a flavor wheel from a defined lexicon.
wheel is divided into several sections to help consumers visualize the different flavo scents, and aromatic qualities found in most CCV products, including SAV, ZAV, and SB However, compared to other food products and beverages, like wine and coffee, mo systematic research is required to construct a practical, accurate CCV sensory wheel.novative statistical and sensory techniques are necessary for constructing a flavor wh from a defined lexicon.

Conclusions and Future Trends
With a long history, traditional CCV derives its distinct flavor from two elemen solid-state fermentation, used in the brewing process; and cereal and bran as raw mate als.However, traditional fermentation in non-sterile, open conditions makes it difficult maintain consistent, stable flavor quality in different CCV batches.The traditional che ical composition and formation mechanism of CCV flavor is gradually revealed due rapid advancements in analytical techniques and molecular sensory science.This stu systemically reviewed the flavor compounds identified in CCV, aroma compound gen ation during the brewing process, and the association between sensory perception a

Conclusions and Future Trends
With a long history, traditional CCV derives its distinct flavor from two elements: solid-state fermentation, used in the brewing process; and cereal and bran as raw materials.However, traditional fermentation in non-sterile, open conditions makes it difficult to maintain consistent, stable flavor quality in different CCV batches.The traditional chemical composition and formation mechanism of CCV flavor is gradually revealed due to rapid advancements in analytical techniques and molecular sensory science.This study systemically reviewed the flavor compounds identified in CCV, aroma compound generation during the brewing process, and the association between sensory perception and key aroma substances.In addition, the CCV lexicon was compiled, and a CCV sensory wheel prototype was constructed.As a result, 101 volatile compounds including acids, pyrazines, furans, aldehydes, esters ketones, alcohols, phenols, were chosen as important aroma compounds of CCV according to the available literature.Primary strains in Daqu and Maillard reaction via Strecker degradation were considered to play a key role in the formation of aroma compounds.A useful sensory wheel was built, including 45 descriptors in 16 categories, for the standardization of the sensory analysis of CCV.
Three areas of research can be pursued in greater detail in the future.First, new analytical techniques can promote the identification of more trace but important aroma compounds.Second, artificial intelligence and machine learning can be employed for flavor quality control based on well-known flavor compound formation mechanisms.Finally, a dynamic, standard sensory wheel should be constructed, like the coffee flavor wheel by the SCAA.

Figure 1 .
Figure 1.A flowchart of the CCV brewing process.

Figure 1 .
Figure 1.A flowchart of the CCV brewing process.

Figure 2 .
Figure 2. A sensory wheel of the CCV.

Figure 2 .
Figure 2. A sensory wheel of the CCV.
a Description from the following database: http://www.flavornet.org(accessed on 8 February 2024).b Each compound was identified based on the following: RI, retention indices; MS, mass spectrometry; S, standard compound injection; odor, the analyte odor descriptions were compared with their corresponding standards, as well as with the literature.

Table 2 .
The descriptive terms and their Chinese translations.