Screening and In Silico Analyses of the Yeast Saccharomyces cerevisiae Σ1278b Bank Mutants Using Citral as a Natural Antimicrobial

The antimicrobial function of citral, one of the main compounds of the essential oils (EO) of the Citrus genus, and widely used by the food industry toward spoilage yeast, was previously proven. In this study, the possible mode of action of citral against yeast cells was evaluated by using a global deletome approach. Firstly, the suitability of Saccharomyces cerevisiae Σ1278b to serve as model yeast was assessed by determining its sensitivity to citral (MIC = 0.5 μL/mL). Subsequently, the complete library of Σ1278b haploid mutants deleted in 4019 non-essential genes was screened to identify potential molecular targets of citral. Finally, the deleted genes in the 590 mutants showing increased citral resistance was analyzed with an in-silico approach (Gene Ontology). The significantly enriched GO Terms were “cytoplasm”, “vacuole”, and “mitochondrion” (cellular components); “catalytic activity” (molecular function); “pseudohyphal growth” (biological process). For molecular function, resistant mutants were grouped into thiosulfate sulfur transferase activity, transferase activity, and oxidoreductase activity; for cellular components, resistant mutants were grouped as: cytoplasm, intracellular organelle, membrane-bounded organelle, mitochondrion, organelle membrane, and vacuole; and finally, with regard to biological process, deleted genes were grouped as: pseudohyphal growth, mitochondrion organization, lipid metabolic process, DNA recombination and repair, and proteolysis. Interestingly, many identified genes were associated with the cellular response to oxidative stress and ROS scavenging. These findings have important implications for the development of citral-based antimicrobials and the elucidation of its mechanism of action.


Introduction
Saccharomyces cerevisiae can endure a reversible developmental transition from a singlecell budding yeast into a multicellular filamentous form [1].This dimorphic shift facilitates haploid cells to form biofilms on a semisolid medium [2] and to penetrate agar in response to carbon deprivation (haploid invasive growth) [3,4], whereas it enables diploid cells to form a bundle of elongated cells known as pseudohyphae in response to nitrogen starvation [5].In the food realm, biofilm formation reduces the quality of dairy products, juices production lines, drinking water systems, beer and wine [6].It has also been shown that the yeast strain Σ 1278b naturally forms biofilm in liquid medium on solid surfaces such as polystyrenes caused by the expression of the protein Flo11p [2,[7][8][9][10].
We are currently facing an increase in the resistance to antifungal molecules, affecting the number of compounds available to control spoilage and pathogenic microorganisms, and producing a need to find new alternatives.Plant extracts contain molecules with antimicrobial activity, among which essential oils offer an alternative to "synthetic agents" [11].These natural compounds are defined to be "generally regarded as safe" (GRAS) by the Foods 2024, 13, 1457 2 of 14 US Food and Drug Administration and offer promising treatments in medicine and applications in the cosmetic, pharmaceutical and food industries [12][13][14][15][16]. Essential oils are known to show biological activity, like antimicrobial and antioxidant activity.They are characterized as complex mixtures of natural compounds [17].In recent years, the antifungal activity of citrus essential oil has been the object of several studies highlighting its use against Aspergillus and Penicillium species [18].
Citral is found in many species of aromatic plants such as lemon myrtle, lemongrass, lemon, orange.This terpenoide is composed of two aldehydes stereoisomers geranial (transcitral) and neral (cis-citral) [19].This component is highly volatile, exhibits a lipophilic action, and presents low solubility in water (Figure 1).Much in vitro research has described the biological activity of citral, including antibacterial activity [20,21], anti-inflammatory activity [22], and antifungal effects [23][24][25][26].Mitropoulou et al. [27] showed that citral interacts with membrane fatty acids of eukaryotic cells such as Geotrichum citri-aurantii.In addition, this component induces mitochondrial morphology, reduces intracellular ATP, and prohibits several enzymes of the tricarboxylic acid pathway of Penicillium digitatum) [18].Bard et al. [28] evaluated the antimicrobial activity of geranial and neral (the two isomers of citral) against S. cerevisiae, which have previously been used as a model system in food spoilage.The environmental stress tolerance mechanism of yeast is based primarily on two factors: cell resistance to environmental stresses and cell self-repair after damage [29].Since oxidative stress primarily causes the accumulation of harmful reactive oxygen species (ROS), the source of ROS and cell response have received considerable attention [30].Shi et al. [20] showed that citral increased the intracellular reactive oxygen species (ROS) allied with alterations in mitochondrial activity, and caused apoptosis cell death by the metacaspase-dependent pathway.In the same study, cell membrane dysfunction was related to the synergy of terpenoids with the lipids of the membrane bilayer [28], inducing leakage of potassium and cell constituents [28,31].Fancello et al. [17] have shown the antifungal effect of Pompia leaf EO on Saccharomyces cerevisiae (E1118) with a MIC of 0.5 µL/mL, and this activity is due to citral, its main component.antimicrobial activity, among which essential oils offer an alternative to "synthetic agents" [11].These natural compounds are defined to be "generally regarded as safe" (GRAS) by the US Food and Drug Administration and offer promising treatments in medicine and applications in the cosmetic, pharmaceutical and food industries [12][13][14][15][16]. Essential oils are known to show biological activity, like antimicrobial and antioxidant activity.They are characterized as complex mixtures of natural compounds [17].In recent years, the antifungal activity of citrus essential oil has been the object of several studies highlighting its use against Aspergillus and Penicillium species [18].Citral is found in many species of aromatic plants such as lemon myrtle, lemongrass, lemon, orange.This terpenoide is composed of two aldehydes stereoisomers geranial (trans-citral) and neral (cis-citral) [19].This component is highly volatile, exhibits a lipophilic action, and presents low solubility in water (Figure 1).Much in vitro research has described the biological activity of citral, including antibacterial activity [20,21], anti-inflammatory activity [22], and antifungal effects [23][24][25][26].Mitropoulou et al. [27] showed that citral interacts with membrane fatty acids of eukaryotic cells such as Geotrichum citriaurantii.In addition, this component induces mitochondrial morphology, reduces intracellular ATP, and prohibits several enzymes of the tricarboxylic acid pathway of Penicillium digitatum) [18].Bard et al. [28] evaluated the antimicrobial activity of geranial and neral (the two isomers of citral) against S. cerevisiae, which have previously been used as a model system in food spoilage.The environmental stress tolerance mechanism of yeast is based primarily on two factors: cell resistance to environmental stresses and cell selfrepair after damage [29].Since oxidative stress primarily causes the accumulation of harmful reactive oxygen species (ROS), the source of ROS and cell response have received considerable attention [30].Shi et al. [20] showed that citral increased the intracellular reactive oxygen species (ROS) allied with alterations in mitochondrial activity, and caused apoptosis cell death by the metacaspase-dependent pathway.In the same study, cell membrane dysfunction was related to the synergy of terpenoids with the lipids of the membrane bilayer [28], inducing leakage of potassium and cell constituents [28,31].Fancello et al. [17] have shown the antifungal effect of Pompia leaf EO on Saccharomyces cerevisiae (E1118) with a MIC of 0.5 µL/mL, and this activity is due to citral, its main component.Citral is an acyclic monoterpenoid aldehyde composed of two geometrical isomers, geranial and neral.This monoterpene is present in many plants, citrus fruits, herbs, and grasses.It is very volatile, exhibits lipophilic activity, and has low solubility in water.
Greater consumer awareness and concern regarding synthetic chemical additives have led different types of industries to control microbial spoilage and hazards using natural preservatives, such as plant essential oils and their components that possess antimicrobial activity.So far, there has been inadequate attention given to the ability of microorganisms to develop resistance to these antibiotic alternatives and limited studies have been conducted to evaluate microbial adaptive response and virulence changes toward sublethal natural compounds exposure.Therefore, new phenotypic and genetic studies are necessary to understand the mechanism of action of natural preservatives to evaluate the possible development of microbial tolerance or resistance to natural compounds.
In this study, we focused on the screening of the bank of mutants of S. cerevisiae Σ1278b for the anti-microbial function of citral.The bank of mutants of S. cerevisiae Σ1278b was created by PCR amplification of the corresponding deletion alleles, from the S288c reference strain collection [33,34].The diploid Σ1278b strain was engineered by holding synthetic genetic array (SGA) mating-type specific reporters [35,36].The library contains about 4019 haploids that each harbor a single deletion in a non-essential gene [37].Thus, the molecular mechanism of the deleted genes in mutants that are able to resist citral helps to understand the antifungal mechanisms of citral.In addition, by an in silico analysis we developed hypotheses that relate the function of the known genes to citral-mediated anti-microbial activity.These efforts contribute new knowledge that will advance our longterm goal of determining the mechanism of action of these molecules, to develop effective methods for preventing unwanted microbial growth, and to deepen our knowledge about the tolerance or resistance of microorganisms to natural compounds.

S. cerevisiae Σ1278b Bank of Mutants
S. cerevisiae Σ1278b haploids were constructed by switching each open reading frame (ORF) in Σ1278b (MATa can1D::STE2p-spHIS5 lyp1D:: STE3p-LEU2 his3::HisG leu2D ura3D) with a kanMX cassette received from the S288c deletion collection [37].Wild Type was used as a reference strain and mutants' alleles were similar to the S288c collection [34].The library consisted of 4019 haploids that each harbor a single deletion in a non-essential gene screened in the Σ1278b strain.The library was replicated in liquid YEPD (Yeast Extract, Peptone, Dextrose) + G418 Sulfate (Gold Biotechnology, St. Louis, MO, USA) + 20% glycerol and stored at −80 • C. The library is not distributed commercially but has been made available to the Department of Agricultural Sciences, Section of Microbiology, (University of Sassari) by Boone Lab, University of Toronto, Canada [38].

Determination of the MIC of Citral on S. cerevisiae Σ1278b
The minimal inhibitory concentration (MIC) was determined as the lowest concentration of citral (3,7-dimethyl-2,6-octadienal, geranial and neral mixture), (Sigma Aldrich, St. Louis, MO, USA), inhibiting visible growth of the tested microorganism.Citral stock solution was first prepared with a concentration of 40 µL/mL.Stock solution was then diluted with sterile water and 1% DMSO, giving a succession of concentrations ranging from 0.078 to 20 µL/mL [17].Overnight culture of the wild-type yeast S. cerevisiae Σ1278b was used to prepare the microbial inoculation used for the test, reaching an OD of 0.2.Aliquots of 100 µL of diluted inoculation at desired cell concentration were added to each well in the 96-well micro-dilution polystyrene plate, which already contained 100 µL of the citral dilution.The plates were then incubated at 30 • C for 24 h.DMSO alone (at 1% concentration) and sterile water was used as positive control.Each experiment was repeated twice.The preculture and dilutions of the parental strain were prepared with an inoculum solution of YEPD (2% bacto-peptone, 1% yeast extract, and 2% glucose) reaching an OD of circa 0.2 (about 150 µL of the preculture for 10 mL of the prepared medium) in YEPD at 30 • C for 24 h.

Screening of the S. cerevisiae Σ1278b Bank of Mutants
Mutants were prepared by growing them individually overnight in rich medium YEPD broth + G418 Sulfate, in 96-well plates for minimizing population bias due to differences in growth fitness that in bulk culture would place slower growing clones at a disadvantage.A copy of the mutants was resuspended in YEPD+20% glycerol, and stored in 200-µL aliquots at −70 • C.This stored copy served as a reference plate to prepare precultures for experiments.Mutants in in 96-well plates were served as inocula for screening of resistance to the antimicrobial activity of citral by incubating them for 24-48 h till growth appearance.The following reagents were mixed and filled with a volume of around 25 mL in Petri plates: 0.1% of DMSO, YEPD agar, in addition to the appropriate concentration of citral (MIC).Once the prepared mixture was solidified on petri plates, the replication from the liquid preculture mutants (kept at 30 • C for 24 h) was performed by using a 48-pin replicator (Figure 2).Each plate containing the mutants was repeated twice and incubated at 30 • C for 24-48 h.
Foods 2024, 13, x FOR PEER REVIEW concentration) and sterile water was used as positive control.Each experiment w peated twice.The preculture and dilutions of the parental strain were prepared w inoculum solution of YEPD (2% bacto-peptone, 1% yeast extract, and 2% glucose) rea an OD of circa 0.2 (about 150 µL of the preculture for 10 mL of the prepared mediu YEPD at 30 °C for 24 h.

Screening of the S. cerevisiae Σ1278b Bank of Mutants
Mutants were prepared by growing them individually overnight in rich me YEPD broth + G418 Sulfate, in 96-well plates for minimizing population bias due ferences in growth fitness that in bulk culture would place slower growing clone disadvantage.A copy of the mutants was resuspended in YEPD+20% glycerol, and in 200-µL aliquots at −70 °C.This stored copy served as a reference plate to pr precultures for experiments.Mutants in in 96-well plates were served as inocu screening of resistance to the antimicrobial activity of citral by incubating them for h till growth appearance.The following reagents were mixed and filled with a volu around 25 mL in Petri plates: 0.1% of DMSO, YEPD agar, in addition to the appro concentration of citral (MIC).Once the prepared mixture was solidified on petri p the replication from the liquid preculture mutants (kept at 30 °C for 24 h) was perfo by using a 48-pin replicator (Figure 2).Each plate containing the mutants was rep twice and incubated at 30 °C for 24-48 h.

Results and Discussion
Growth of S. cerevisiae Σ1278b in the presence of different concentrations of citral was firstly carried out to assess the suitability of this strains as a model-strain to study the mechanisms of action of citral in yeast.Interestingly, the here-identified MIC of citral against Σ1278b (0.5 µL/mL) was identical to that already described in the literature against other yeast strains [17].Subsequently, the complete library of Σ1278b haploid mutants Foods 2024, 13, 1457 5 of 14 deleted in 4019 non-essential genes was screened by using the MIC of citral determined on the wild-type strain.Systematic phenotypic and genetic analysis with the deletion mutant collection has proven highly useful because it allows thorough examination of mutations in each gene for specific phenotypes [37].Thus, analyzing the resistance or sensitivity of the mutants toward the citral could lead to an understanding of the mechanisms or pathways behind citral action on yeast.Among the tested 4019 mutants, 509 proved to be resistant to citral (Table S1).To identify molecular functions, biological processes or cellular localizations common to the identified mutants, the list of the genes deleted in the resistant mutants was analyzed by using the Gene Ontology (GO) "TermFinder" and "Slim Mapper" as implemented in the Saccharomyces Genome Database website [39].GO TermFinder is used to significantly identify highly enriched annotations among genes in major subclusters, while GO Slim Mapper maps granular annotations to high-level parent GO Slim terms.Figure 3 and Table 1 show the enrichment analysis findings and the significantly enriched GO categories, respectively.

Results and Discussion
Growth of S. cerevisiae Σ1278b in the presence of different concentrations of citral was firstly carried out to assess the suitability of this strains as a model-strain to study the mechanisms of action of citral in yeast.Interestingly, the here-identified MIC of citral against Σ1278b (0.5 µL/mL) was identical to that already described in the literature against other yeast strains [17].Subsequently, the complete library of Σ1278b haploid mutants deleted in 4019 non-essential genes was screened by using the MIC of citral determined on the wild-type strain.Systematic phenotypic and genetic analysis with the deletion mutant collection has proven highly useful because it allows thorough examination of mutations in each gene for specific phenotypes [37].Thus, analyzing the resistance or sensitivity of the mutants toward the citral could lead to an understanding of the mechanisms or pathways behind citral action on yeast.Among the tested 4019 mutants, 509 proved to be resistant to citral (Table S1).To identify molecular functions, biological processes or cellular localizations common to the identified mutants, the list of the genes deleted in the resistant mutants was analyzed by using the Gene Ontology (GO) "TermFinder" and "Slim Mapper" as implemented in the Saccharomyces Genome Database website [39].GO Term-Finder is used to significantly identify highly enriched annotations among genes in major subclusters, while GO Slim Mapper maps granular annotations to high-level parent GO Slim terms.Figure 3 and Table 1 show the enrichment analysis findings and the significantly enriched GO categories, respectively.Manhattan plot grouping molecular functions (in red), biological processes (in orange), cellular components (in green).The x-axis displays functional words that have been color-coded and grouped by data source.The circle sizes correspond to the phrase size, so larger terms have larger circles, implying a greater number of genes.The y-axis displays the adjusted enrichment p-values in negative log10 scale.The meanings of the numbers are given in Table 1.In the "molecular function" main category, the sets 'catalytic activity' and 'binding' do not intersect.In "biological processes", the sets 'primary metabolic process' and 'filamentous growth' intersect, as do the sets 'growth of unicellular organisms' and 'organic substance metabolic process'.In "cellular component", 'mitochondrion' and 'organelle membrane' intersect.This mean that these structures hold genes in common.As shown in the plot, the intracellular anatomical structure is separated from other biological processes, while the latter is significantly closed to the cytoplasm.
To assess the grade of comparability of the phenotypic profiles of individual deletion strains, and consequently the likely gene products in various structures, we progress deeply with each of the three structures: molecular function, cellular component, and biological process.

Molecular Function of the Identified Genes
A "molecular process" is defined as a process that can be performed by a single macromolecular machine, typically through direct physical interactions with other molecular entities.In this context, it refers to an action or activity carried out by a gene product (or a complex) (Table 2).Among the 590 genes, 242 genes were grouped by catalytic activity with a p value of 0.00802, and four genes were grouped by thiosulfate sulfur transferase activity (p = 0.09019), where YOR286W, YOR251C, YOR285W, YHR111W have the two common functions.Concerning other child terms, 21 genes were grouped as transferase activity, and 38 genes as oxidoreductase activity (Table 2).Oxidoreductase activity is a child term of catalytic activity known as part of a large family of enzymes that catalyze oxidoreduction responses, such as electron transfer, proton/hydrogen extraction, hydride transfer, oxygen insertion, and the exchange of electrons between the donor and acceptor molecules.

Biological Process of the Identified Genes
A "biological process" is defined as a specific target for which the organism is genetically programmed (SGD).Among 590 resistant mutants, the corresponding deleted genes are grouped as pseudohyphal growth (p = 0.02426), followed by regulation of pseudohyphal growth (p = 0.04274), and cell growth.Table 3 summarizes the map annotations of broad categories, using the GO Slim Mapper.Cluster frequency indicates that genes are close to each other under the same process, while "genome frequency" is the degree that a group of genes appear in a specific process when information is passed from parent to child in a cell.These two frequencies are associated in parallel, beginning with pseudohyphal growth, regulation of pseudohyphal growth, cell growth, growth of unicellular organism as a thread of attached cells, lipid metabolic process, ending with regulation of filamentous growth.4 shows the child terms of the biological process considered most significant.Mitochondrion organization (36 genes), mitotic cell cycle (32 genes,) and meiotic cell cycle (30 genes) were identified as important child terms of biological process.Other identified pathways pertained to lipid metabolic processes (52 of 590 genes), nucleobase-containing small molecule metabolic processes (20 of 590 genes), DNA recombination (19 of 590 genes), proteolysis involved in protein catabolic processes (8 of 590 genes) and DNA repair (25 of 590 genes).The knockout of these genes led to the survival of the corresponding mutants in the presence of citral, meaning that the indicated processes are potential targets of the antimicrobial action of citral.In particular, the perturbance of pseudohyphal growth and lipid metabolic processes seem to be linked to the resistance of the yeast mutants to citral.Other child terms of biological process are related to the growth of unicellular organisms as a thread of attached cells, filamentous growth of a population of unicellular organisms, mitochondrion organization, the mitotic cell cycle, the meiotic cell cycle, meaning that the perturbance of these processes led to the survival of the mutants.Among the different cellular processes, genes here identified and related to the cellular response to oxidative stress (mitochondrion organization) were further considered.Indeed, it has been previously suggested that citral increases the intracellular reactive oxygen species (ROS) [20].

Genes Related to the Cellular Response to Oxidative Stress
Oxidative stress is recognized as any process that alters a cell state or activity (such as movement, secretion, production of enzymes, gene expression) because of oxidative pressure, a state frequently resulting from exposure to elevated degrees of reactive oxygen species, for example superoxide anions, hydrogen peroxide (H 2 O 2 ), and hydroxyl radicals (https://www.yeastgenome.org/(accessed on 14 January 2024)).Table 5 lists genes related to cellular response to oxidative stress, and whose deletion confers Σ1278b resistance to citral.These genes are discussed individually in the following section.Hydrogen peroxide menadione oxidative stress resistance decreased [62] fRMsr as well as MsrA and MsrB have antioxidant functions in yeast and regulate oxidative stress.MsrA and MsrB were discovered to regulate mitochondrial activities in yeast by the cellular mechanism for methionine sulfoxide reduction [42].These enzymes, however, had differential effects on mitochondrial activity, with MsrA largely found in the cytoplasm and MsrB in mitochondria.Kaya et al. [63] reported the sensitivity of ∆ykl069w to H 2 O 2 , leading to cell death.
AFG1 and PUF3 have a physical interaction whereas Puf3p (citral enables this function) is expected to play a function in translation control under oxidative stress conditions, with its role in repression of protein synthesis linked to mitochondrially active genes [64].The minimal phenotypic manifestation in ∆afg1 cells suggests that yeast mitochondria are resistant to a large degree of mitochondrial depolarization and ROS [41].
As mentioned by Gebert et al. [65], the knockout of GRE3 leads to the increase of the pentose phosphate pathway, through the reactions that detoxify ROS with glutathione and thioredoxin.In addition, ∆gre3 cells experienced a significant increase in H 2 O 2 and O 2 concentrations during chronological ageing in comparison to wild-type yeast cells.
TIM18 is located in mitochondria and is part of the TIM22 mitochondrial import inner membrane insertion complex [65].Nakamura et al. [52] showed that yeast's response to apoptotic stress and other type of stresses is affected by TIM18 deficiency, and that this gene is considered a downstream component of ROS formation.In addition, the ∆tim18 mutant was resistant to some stresses that stimulate apoptosis, such as hydrogen peroxide.
∆gpx1 showed sensitivity to all stresses tested, such as H 2 O 2 , and this gene showed importance for yeast growth under oxidative stress [61].However, it has also been suggested that MPR1 probably compensates for the role of GPX1 in the deleted mutant, by reducing intracellular ROS accumulation.
As reported by Lee et al. [62], deletion of ∆mcr1 proved resistant to an antimicrobial hexapeptide.
SRX1 enhances oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p.Srx1p and Prx1p have a physical interaction, where PRX1 is a mitochondrial peroxiredoxin with thioredoxin peroxidase activity, induced during oxidative stress and reactivation requires Trr2p and glutathione [58].
Costa et al. [66] and MacDiarmid et al. [49] found an increased sensitivity of ∆trr2 to H 2 O 2 , suggesting the important role of the mitochondrial thioredoxin reductase in protection against oxidative stress in S. cerevisiae.The mitochondrial thioredoxin proteins thioredoxin (Trx3p) and thioredoxin reductase (Trr2p) have been linked to oxidative stress defense during respiratory metabolism [59].
TSA1 codifies for a peroxiredoxin that reduces hydrogen peroxide and organic hydroperoxides by using electrons from the thioredoxin/thioredoxin reductase pathway.The ∆tsa1 mutant was proven to be sensitive to H 2 O 2 and ethanol [48,[66][67][68][69]. Another study showed that the ∆trr1 increased oxidant tolerance in a ∆tsa1 mutant.However, it was confirmed that TRR1 significantly reduced resistance to hydrogen peroxide, either alone or in combination with ∆tsa1.When two conserved cysteines create an intramolecular disulfide bond, they exist in either a reduced form (thioredoxin-(SH)2) or an oxidized form (thioredoxin-S2). Thioredoxins transfer electrons from their active center dithiol to disulfide bonds in proteins (Protein-S2), which are then reduced to dithiols (Protein-(SH)2).TRR1 directly reduces the resultant oxidized thioredoxin disulfide with electrons provided by NADPH.Thioredoxin, thioredoxin reductase, and NADPH comprise the thioredoxin reduction system.Oxidized glutaredoxins, on the other hand, are reduced using electrons given by NADPH by the tripeptide glutathione (gamma-Glu-Cys-Gly, or GSH).Thus, glutaredoxin, glutathione, glutathione reductase, and NADPH comprise the glutathione/glutaredoxin system [70].The expression of TRR1 is regulated by the transcription factors Yap1p and Skn7p in response to H 2 O 2 [71].
Park et al. [47] demonstrated that HSP31 is involved in the protection against reactive oxygen species (ROS) and that oxidative stress induces its expression in a Yap1p-dependent manner.This could be explained by the possibility that the induction of the Yap1p gene is dependent on the presence of functional Yap1p or Hsp31p synthesis, which is regulated independently of Yap1p by the level of intracellular ROS.SOD1 and HSP31 may complement each other [45], and it was demonstrated that oxidative stress induces HSP31 expression.Several studies [72][73][74] have found that Yap1p is involved in the upregulation Foods 2024, 13, 1457 11 of 14 of oxidative stress response genes after exposure to reactive oxygen species.Yap1p binding sites are found in the promoters of most genes that are strongly induced in response to ROS, including cytosolic catalase (CTT1), cytosolic superoxide dismutase (SOD1), gamma glutamylcysteine synthetase (GSH1), thioredoxin 2 (TRX2), and thioredoxin reductase (TRR1) [50,75].The formation of an intramolecular disulfide in one or more cysteine-rich domains is thought to trigger Yap1p redistribution from the cytoplasm to the nucleus [76].

Conclusions
In conclusion, based on the above results, the identified 509 mutants resistant to citral were subdivided into categories depending on GO biological process, molecular function, and cellular component of the deleted genes.The categories "mitochondrion organization" (36 genes), "mitotic cell cycle" (32 genes), and "meiotic cell cycle" (30 genes) comprised the highest number of mutants.Furthermore, many of the identified genes were related to pathways responsible for oxidative stress, such as ROS scavenging, and could be involved in different mechanisms.In particular, the antimicrobial activity of citral may concern the induction of Yap1p, the increase in the level of intracellular ROS, the decrease of the pentose phosphate pathway, or the triggering of other proteins involved in the membrane-embedded core of the TIM22 complex.Thus, it is conceivable that citral is targeting the antioxidant response of the cell.Also, citral might have a role in stimulating the thioredoxin system, by mitochondrial peroxiredoxin (PRX1), and the mitochondrial thioredoxin proteins thioredoxin (Trx3p) and thioredoxin reductase (Trr2p), that are linked to oxidative stress defense.Also, citral is probably increasing the role of TRR1 (thioredoxin reductase).By analyzing the Σ1278b S. cerevisiae mutant-gene bank, we contributed for first time to the molecular biology knowledge of the mechanisms of action of antimicrobial molecules, with particular emphasis on the resistance produced by natural or induced mutations, and investigated effective methods for preventing unwanted microbial growth, especially in food matrices, promoting the possibility that citral may be used safely and efficiently, and helping to understand which mechanisms underlie the development of tolerance or resistance of microorganisms to citral.

Figure 3 .
Figure 3. Manhattan plot grouping molecular functions (in red), biological processes (in orange), cellular components (in green).The x-axis displays functional words that have been color-coded and grouped by data source.The circle sizes correspond to the phrase size, so larger terms have larger circles, implying a greater number of genes.The y-axis displays the adjusted enrichment pvalues in negative log10 scale.The meanings of the numbers are given in Table1.

Figure 3 .
Figure 3. Manhattan plot grouping molecular functions (in red), biological processes (in orange), cellular components (in green).The x-axis displays functional words that have been color-coded and grouped by data source.The circle sizes correspond to the phrase size, so larger terms have larger circles, implying a greater number of genes.The y-axis displays the adjusted enrichment p-values in negative log10 scale.The meanings of the numbers are given in Table1.

Table 1 .
Term name of cellular components (CC), biological processes (BP) and molecular functions (MF).

Table 2 .
Important child terms of MF.

Table 3 .
Classification of deleted genes according to biological process.

Table 4 .
Important child terms of biological process.

Table 5 .
Summary of previous studies related to deleted genes in selected resistant mutants.Chemicals such as hydrogen peroxide, paraquat, and menadione were used in prior studies to study their resistance or sensitivity in the yeast.