Evaluation of Autochthonous Coagulase—Negative Staphylococci as Starter Cultures for the Production of Pastırma

The aim of the study was to investigate the effects of Staphylococcus xylosus 39, S. equorum 53, or S. vitulinus 75, previously isolated from pastırma, on the quality characteristics of pastırma, a Turkish dry-cured meat product, and to evaluate their potential use as starter cultures. The pastırma production was carried out with a traditional method. The control pastırma groups were manufactured without adding any starter culture. At the end of production, the groups were subjected to microbiological and physico-chemical analyses. The pH was above 5.5, and the aw value was below 0.90 in all groups. The strains used exhibited good adaptation to the pastırma. The S. equorum 53 decreased the thiobarbituric acid reactive substances (TBARS) value in pastırma, while the S. xylosus 39 increased the redness (a*) color value. The autochthonous strains caused a decrease in the palmitic acid (C16:0). However, they had no significant effect on the stearic acid (C18:0) and the oleic acid (C18:1n-9c). A total of 41 volatile compounds were identified in the groups. S. vitulinus 75 increased both benzaldehyde and 2-methyl-3-phenylpropanal levels. In addition, the principal component analysis (PCA) of volatile compounds provided a good separation, and PC1 separated S. xylosus 39 from other groups.


Introduction
Pastırma, the most representative Turkish dry-cured meat product, is made by curing, drying, and applying a paste called çemen to whole muscles obtained from certain parts of beef or water buffalo carcasses [1]. The traditional production is carried out under natural conditions depending on climate and weather conditions in late September and October to November [2]. Gram positive, catalase-positive cocci have an important share in the microbiota of pastırma [1][2][3][4]. Coagulase-negative staphylococci (CNS) constitute an important part of these microorganisms [4,5]. It is stated that CNS are technologically important microorganisms in whole-muscle meat products such as ham and pastırma [6,7]. CNS are involved in the formation and stability of color via nitrate reductase activity, which leads to the nitrosomyoglobin formation in cured meat products [8][9][10][11][12]. Nitrite formed by reducing nitrate can also limit lipid oxidation [10,12]. Moreover, these microorganisms play an important role in preventing lipid oxidation by breaking down H 2 O 2 with catalase and superoxide dismutase activities [10]. On the other hand, CNS play a role in flavor development by forming low molecular weight compounds with their proteolytic and lipolytic activities [7,9,10].
It is very important to choose a starter culture suitable to obtain a quality product. Starter cultures that are not suitable for the product process may adversely affect the sensory properties of the product [13][14][15]. Meat-starter cultures are divided into two categories. The first-generation starter cultures are generally from vegetable fermentation

Materials
M. longissimus thoracis et lumborum was used for the pastırma production. The muscles obtained from two carcasses (36 months old, male cattle, Brown Swiss crossbred) (24 h post mortem) were taken from the Meat and Dairy Board Erzurum, Türkiye.
Fenugreek (Trigonella foenum graecum L.) seed flour, fresh garlic, red sweet pepper, and red hot pepper were obtained from local markets in Erzurum, Türkiye.
Each strain to be used in the production of pastırma (S. xylosus 39, S. equorum 53, or S. vitulinus 75) was transferred to 100 mL nutrient broth (Oxoid, Basingstoke, Hampshire, UK) in a 300 mL erlenmeyer flask as 1 mL and then incubated at 37 • C for 24 h in the shaking at 180 rpm (TH-30, Edmund Bühler, Bodelshausen, Germany). After the incubation, the concentrated cells obtained with centrifugation (2500× g, 4 • C, and 10 min) (Thermo Fisher Scientific, MR23G, Waltham, MA, USA) were washed 2-3 times with 0.85% (w/v) sodium chloride (NaCl) solution. The concentrated cell was resuspended with 1 mL 0.85% NaCl solution, and the cell number per mL was determined on Nutrient Agar (Oxoid) [27]. A cell suspension with approximately 1 × 10 10 CFU/mL was obtained. In view of this result, it was repeated for each strain before each production.

Pastırma Production
Pastırma production was carried out under natural conditions using the traditional method in a local plant (Kadakçıoglu Meat and Meat Products, Erzurum, Türkiye). After removing fat and connective tissue from the surface, each muscle was divided into two parts, and four meat pieces were obtained from one carcass. The meat pieces obtained from the same carcass were used for a repetition. A total of 8 meat pieces were used for two treatments. In each production, one piece was evaluated as a control group, and strain inoculation was not performed. The other three pieces were applied separately with S. xylosus 39, S. equorum 53, or S. vitulinus 75. In the inoculation, 1 mL of cell suspension (approx. 1 × 10 10 CFU/mL) was used for each surface of pieces and before use, the cell suspension was resuspended with 20 mL of 0.85% NaCl solution. Then, the suspension prepared was spread on the surface of pieces. After one hour, the pieces were cured with a curing mixture (80 g NaCl, 3 g saccharose, and 0.3 g potassium nitrate per kg meat) at 9-10 • C for 48 h. After surfaces of the pieces were lightly washed with tap water, the pieces were hung for 6 days at 15-25 • C (first drying). Afterward, they were subjected to the first pressing at 10 • C for 20 h, following which they dried again for 5 days at 18-22 • C (second drying). After the drying, second pressing was carried out at 20-22 • C for 1.5-2 h. After this procedure, the third drying was carried out for 5 days at 15-20 • C. Then, dried pieces were put in a bowl of paste (çemen) containing 500 g flour (Trigonella foenum graecum) ground from seed, 350 g fresh garlic, 75 g red sweet pepper, 75 g red hot pepper, and 1200 mL water for a day at 10 • C. The paste on the surface of the meat pieces was trimmed to a 3-4 mm thick layer of paste on each piece. Finally, the paste-covered product was dried at 15-20 • C for 10 days.

Determination of the pH, Water Activity (a w ), and Thiobarbituric Acid-Reactive Substances (TBARS) Values
Ten g of sample was homogenized with 100 mL of distilled water using an Ultra-Turrax (IKA T25, IKA Werke GmbH & Co, Breisgau, Germany). The pH value was measured with a pH meter (Thermo Fisher Scientific, Orion Star, Waltham, MA, USA).
The a w value was determined using a water activity device at 25 • C (Novasina TH-500 a w Sprint, Pfaffikon, Switzerland).
TBARS value was determined using the method given by Lemon [28]. TBARS value was given as mg malondialdehyde (MDA)/kg.

Microbiological Analyses
Twenty-five g samples were weighed into sterile Stomacher bags and added to 225 mL of sterile saline water (0.85% NaCl). The homogenization was performed for 2 min in Stomacher (Lab Stomacher Blander 400-BA, London, UK). Serial dilutions were prepared, and the spread-plate method was used for enumeration of Micrococcus/Staphylococcus and Enterobacteriaceae counts.
Mannitol Salt Phenol Red (MSA, Oxoid) agar was used for Micrococcus/Staphylococcus. Incubation was carried out at 30 • C for 2 days under aerobic conditions. Enterobacteriaceae were determined on Violet Red Bile Dextrose (VRBD, Merck, Darmstadt, Germany) agar. The plates were incubated at 30 • C for 2 days under anaerobic conditions.

Determination of Color Values
After the pastırma samples were sliced into slices 1.5 mm in thickness, L*, a*, and b* values were measured with a Chroma Meter (CR-400 Konika Minolta, Osaka, Japan) with an aperture size of 8 mm, a *C D65 illuminant, and standard observed of 2 • . All measurements were made in triplicate.

Determination of Fatty Acid Composition
One g of pastırma sample was transferred to the centrifuge tube, and 20 mL of the solvent (2:1 chloroform:methanol + 0.25 g/L butylated hydroxytoluene) was added to it. After it was subjected to homogenization with an Ultra-Turrax homogenizer for 1 min, it was filtered with a nuche Erlenmeyer, and 4 mL MgCl 2 ·6H 2 O was added to it. After the sample in the tube was treated with nitrogen, it was vortexed and kept in the dark for 24 h, and the lower phase was transferred [29]. The methylation process of fatty acids was performed according to Metcalfe and Schmitz [30]. The samples taken into vials were kept at −18 • C until analysis. GC/FID (Gas Chromatography/Flame Ionization Detector, Agilent Technologies 6890 N, Wilmington, DE, USA) was used to determine the fatty acid composition. In the system, CPSIL 88 (Agilent 100 m × 250 µm × 0.20 µm) was used as a column. The oven temperature was gradually increased from 100 • C to 225 • C. Helium was used as a carrier gas with a 1.2 mL/min flow rate. A fatty acid methyl ester mix (Supelco, FAME-mix, 47885, Bellefonte, PA, USA) was used as the standard, and the results were given as percentage.

Determination of Volatile Compounds
Five g of sample were weighed into 40 mL vials. After the vials were kept in a 30 • C thermal block for 1 h, the carboxen/polydimethylsiloxane (CAR/PDMS) fiber (Supelco) was inserted into the vial for the extraction of volatile compounds using the solid-phase microextraction technique. The fiber was kept in the vial at the same temperature for 2 h, and the extraction process was completed. Gas chromatography (GC, Agilent Technologies 6890N)/mass spectrometry (MS, Agilent Technologies 5973) was used to identify volatile compounds. DB-624 (J&W Scientific, 60 m × 0.25 mm × 1.4 µm film) was used as the column in the system. The oven temperature was initially at 40 • C for 5 min; then it was gradually increased from 110 • C to 210 • C and kept at this temperature for 12 min. Helium was used as a carrier gas in the system at a flow rate of 1 mL/min. In the identification of compounds, the library of mass spectrometry (NIST, WILEY, and FLAVOR) and standard mix (n-Paraffins Mix, Supelco 44585-U) were used, and the standard mix was also used to determine the Kovats index. The results were given in AU × 10 6 [1].

Statistical Analyses
The analysis of variance (ANOVA) was performed using a general linear model with treatment as a main effect and replications (blocks) as a random effect in a randomized complete block design. Experiments were carried out in two repetitions. The individual standard deviations of the means are presented in the tables. Differences between the means were determined during Duncan's multiple range tests (p < 0.05). The statistical analyses were performed using the SPSS version 20 statistical program (SPSS Inc., Chicago, IL, USA). Principal component analysis (PCA) was also performed using the Unscrambler software (CAMO version 10.1, Oslo, Norway) to determine the relation between the autochthonous strains and volatile compounds or fatty acid compositions.

pH and a w Values
The highest pH was found in the pastırma with S. equorum 53. No significant difference in pH values was observed between the control group and the group with S. vitulinus 75 (p > 0.05) (Table 1). Similarly, Aksu and Kaya [19] determined that the commercial preparations containing S. carnosus did not cause a significant change in the pH value of pastırma. In another study conducted by Bosse et al. [27], the pH of dry-cured ham containing S. carnosus LTH 3838 or S. carnosus LTH 7036 strains was higher than the control group, but there was no statistically significant difference between the groups. According to the results obtained from the present study, S. equorum 53 and S. xylosus 39, among the autochthonous staphylococci strains, gave slightly higher average pH values compared to the control, but the pH values measured in all groups are within the acceptable limits [31].
The use of autochthonous strains had a significant effect (p < 0.05) on the a w value. The groups containing S. xylosus 39 or S. vitulinus 75 showed a higher a w value compared to the control group (Table 1). On the contrary, Bosse et al. [27] determined a lower average a w value in dry-cured raw samples containing S. carnosus LTH 3838 and S. carnosus LTH 7036 strains compared to the control. It was also emphasized that there was no significant difference between S. carnosus strains. a w is an important hurdle effect for the microbiological stability of pastırma. However, for sensory reasons, the a w value of pastırma should be between 0.85 and 0.90 [1]. In our study, the a w value was found in this range in all groups.

Thiobarbituric Acid-Reactive Substances (TBARS)
The use of autochthonous strains had a significant effect on the TBARS value of pastırma (p < 0.05) ( Table 1). The highest mean TBARS value was determined in the group inoculated with S. vitulinus 75; however, this value did not differ statistically from the mean values of the control and S. xylosus 39 group (p > 0.05). The lowest mean TBARS value was determined in the presence of S. equorum 53 (p < 0.05), and this value did not differ from the average value of the S. xylosus 39. Aksu and Kaya [21] determined that a commercial starter culture containing S. carnosus showed a low TBARS value during pastırma production. It is thought that the strains used in this study, especially the S. equorum 53 strain, showed lower TBARS values compared to the control due to the nitrate reductase and catalase enzyme activities [4]. Talon et al. [32] also stated that staphylococci have an antioxidant property and some strains of S. xylosus exhibit superoxide dismutase activity. Moreover, Landeta et al. [12] indicated that the S.vitulinus IFJ 4 strain exhibited stronger catalase activity compared to the S. equorum IFJ 25 strain. There may be a difference even among different strains of the same species in terms of catalase activity, and S. xylosus strains also shows significant differences in terms of catalase activity. The unsaturated fatty acids formed as a result of the activity of lipolytic enzymes are good precursors for autoxidation. In a previous study, the autochthonous strains used in the present study were found to have lipolytic activity [4]. However, it was determined that the strains caused differences in TBARS values. This result showed that the lipolytic activities of the strains are affected differently by the process conditions.

Microbiological Properties
In the study, strains used as starter cultures in pastırma showed good adaptation to production conditions. A higher average count of Micrococcus/Staphylococcus was determined in the pastırma groups produced using S. xylosus 39 and S. equorum 53 compared to the control group and the group with S. vitulinus 75 ( Table 1). As can be seen from Table 1, the S. vitulinus 75 strain showed a mean value of 7.26 ± 0.19 log CFU/g. However, this value did not differ statistically from the control group. CNS are the predominant microorganisms in pastırma. These microorganisms affect the sensory properties of pastırma with their catalase, nitrate reductase, proteolytic, and lipolytic activities [3,4,25,26].
The Enterobacteriaceae count was found to be below the detectable limit in all groups. It is emphasized that the inability of these family members to survive is due to curing/salting and a decrease in water activity as a result of drying/ripening [1]. Furthermore, in many studies on pastırma, it was reported that the members of the Enterobacteriaceae family were below 2 log CFU/g [1,20,33].

Color Values
There was no difference in terms of the mean L* value between the group containing S. xylosus 39 and the control group (Table 1). Likewise, the other two strains did not cause a significant change in the L* value compared to the control group (Table 1). In contrast, Bosse et al. [27] reported that the S. carnosus LTH 3838 strain caused a decrease in the L* value of dry-cured ham.
CNS are microorganisms effective in color formation with nitrate reductase activities. The group with S. xylosus 39 showed the highest average a* value. Similarly, the use of S. vitulinus 75 caused an increase in a* value. Bosse et al. [27] also reported that the S. carnosus LTH 3838 strain increased the a* value in cured raw ham samples. On the other hand, S. equorum 53 did not cause a significant change in the a* value compared to the control (p > 0.05). These results can be evaluated as a good indicator that S. xylosus 39 and S. vitulinus 75 may show strong nitrate reductase activity in the production process of pastırma. It is also known that CNS can eliminate the adverse effects of this compound on color by breaking down hydrogen peroxide with catalase activities. The two most remarkable species in terms of color formation in industrial production are S. xylosus and S. carnosus [34]. Similarly, Sørensen [35] stated that S. xylosus, used as a starter culture in dry-cured meat products, was an important species in terms of color formation and stability. Likewise, Stahnke [36] reported that S. xylosus, commonly found in the natural microbiota of fermented sausages, was an important species in terms of color formation by reducing nitrate to nitrite. Prpich et al. [37] also determined that the S. vitulinus strain (S. vitulinus C2), having nitrate reductase activity, was more effective in the formation of more intense red color compared to the mixed culture (S. vitulinus C2 and L. sakei 487). In the present study, it is thought that the reason why S. equorum 53 strain has the lowest average a* value is probably due to lower nitrate reductase activity compared to others. Indeed, Landeta et al. [12] reported that there was a considerable difference between S. equorum strains in terms of nitrate reductase activity. The lowest average b* value in the pastırma samples was found in the group containing S. equorum 53, and this average value did not differ statistically from the group without a starter culture (control) (p > 0.05). There were statistical differences (p < 0.05) between the group with S. xylosus 39, in which the highest average b* value was found, and other groups (Table 1). In contrast, Aksu and Kaya [19] stated that the commercial starter culture application did not have an effect on the b* value in the production of pastırma.

Fatty Acid Composition
The use of autochthonous strains showed different effects on the proportions of many fatty acids ( Table 2). The highest average values of myristic (C14:0) and myristoleic (C14:1) acids were determined in the group with S. xylosus 39, but these average values differed statistically only from the control group (p < 0.05) ( Table 2). The proportion of palmitic acid (C16:0), which is an important saturated fatty acid in meat and, thus, in pastırma, varied between 26.66 ± 0.94% and 30.92 ± 1.10% in the groups. The control group had the highest mean proportion of palmitic acid (C16:0). There was no statistically significant difference between the groups with autochthonous strains in terms of palmitic (C16:0). The highest proportion of palmitoleic acid (C16:1) was found in the control group (p > 0.05) ( Table 2). However, no significant difference between the control group and the group with S. vitulinus 75 was determined ( Table 2). In pastırma groups, the proportion of palmitoleic acid (C16:1) varied between 3.49 ± 0.39% and 2.86 ± 0.54%. Similar results were observed in a study conducted on pastırma by Çakıcı et al. [38]. Palmitic acid (C16:0), stearic acid (C18:0), and oleic acid (C18:1n-9c) were found to be the dominant components of the fatty acids in all pastırma groups ( Table 2). The stearic acid (C18:0) in pastırma was not affected by the use of autochthonous strains ( Table 2). On the contrary, Aksu and Kaya [21] reported that commercial starter cultures (S. carnosus, S. carnosus + Lactiplantibacillus pentosus or S. xylosus + Latilactobacillus sakei) caused an increase in this fatty acid. The stearic acid (C18:0) proportions in this study were similar to the results reported in other studies on pastırma [21,38].
Principal component analysis (PCA) was applied to assess the relationships between the strains (S. xylosus 39, S. equorum 53, and S. vitulinus 75) and the fatty acid composition (Figure 1). The first PC was enough to explain 90% of the variation for fatty acid composition. In other words, the first two principal components explained 98% of the total variance ( Figure 1). The control and the group with S. equorum 53 placed on the positive side of PC1, while the group with S. xylosus 39 and S. vitulinus 75 placed on the negative side of PC1 ( Figure 1). As can be seen in Table 2, there is no difference between the control group and S. equorum 53 in terms of myristic (C14:0) and myristoleic (C14:1) acids (Table 2). According to the results of PCA, the use of different strains caused changes in fatty acids. The myristic (C14:0) and myristoleic (C14:1) acids were more related to using S. xylosus 39 and S. vitulinus 75 in the negative side of PC1. The palmitic acid (C16:0), stearic acid (C18:0), and oleic acid (C18:1n-9c) were located on the positive side of PC1 and showed a positive correlation with the control and S. equorum 53 (Figure 1). acids. The myristic (C14:0) and myristoleic (C14:1) acids were more related to using S. xylosus 39 and S. vitulinus 75 in the negative side of PC1. The palmitic acid (C16:0), stearic acid (C18:0), and oleic acid (C18:1n-9c) were located on the positive side of PC1 and showed a positive correlation with the control and S.equorum 53 (Figure 1).  Table 2).

Volatile Compounds
In the pastırma groups, 41 volatile compounds belonging to a total of 11 different groups, including 6 sulfur compounds, 2 alcohols, 6 ketones, 8 aliphatic hydrocarbons, 2 esters, 10 aldehydes, 2 aromatic hydrocarbons, 1 nitrogen compound, 2 furans, 1 terpene, and 1 acid, were identified. The use of autochthonous strains did not show a statistically significant difference on sulfur compounds (p > 0.05) ( Table 3). In the study conducted by Kaban [1] on determining volatile compounds in pastırma, allyl methyl sulfide andmethyl-2-propenyl disulfide compounds were determined, and it was emphasized that garlic in the fenugreek composition was the source of many sulfur compounds. Sulfur compounds may also originate from methionine, cysteine, and cystine [39].   Table 2).

Volatile Compounds
In the pastırma groups, 41 volatile compounds belonging to a total of 11 different groups, including 6 sulfur compounds, 2 alcohols, 6 ketones, 8 aliphatic hydrocarbons, 2 esters, 10 aldehydes, 2 aromatic hydrocarbons, 1 nitrogen compound, 2 furans, 1 terpene, and 1 acid, were identified. The use of autochthonous strains did not show a statistically significant difference on sulfur compounds (p > 0.05) ( Table 3). In the study conducted by Kaban [1] on determining volatile compounds in pastırma, allyl methyl sulfide andmethyl-2-propenyl disulfide compounds were determined, and it was emphasized that garlic in the fenugreek composition was the source of many sulfur compounds. Sulfur compounds may also originate from methionine, cysteine, and cystine [39].  determined in the group with the S. equorum 53 (p < 0.05). This group was followed by S. vitulinus 75 (Table 3). These compounds were also determined in the dry sausage model system containing S. equorum 19 and S. saprophyticus 11 [42]. In a study comparing the aroma production of S. xylosus, S. carnosus, and S. equorum in model systems, it was stated that S. xylosus and S. equorum cause an increase in the levels of methyl-branched ketone as a result of the degradation of leucine, isoleucine, and valine amino acids [43]. Aliphatic hydrocarbons do not have a significant effect on aroma in dry-cured meat products due to high threshold values [1]. In the current study, the use of autochthonous strains did not have a significant effect on aliphatic hydrocarbons (p > 0.05) ( Table 3). In the study, propyl hexanoate and hexyl butanoate compounds were identified; however, no significant differences were observed between the groups (p > 0.05) ( Table 3).
Aldehydes constitute an important group of aroma compounds due to their low threshold values. These compounds form an important part of the volatile profile in pastırma [1,33]. In the current study, among aldehydes, acetaldehyde, hexanal, and nonanal were the most abundance compounds (Table 3). Hexanal, a dominant compound in pastırma, is formed from the oxidation of omega-6 fatty acids (linoleic and arachidonic acid), and its high concentrations cause a rancid taste in meat products [2]. S. vitulinus 75 showed the highest values for both benzaldehyde and 2-methyl-3-phenylpropanal (Table 3). According to these results, S. vitulinus 75 is an important strain for both benzaldehyde and 2-methyl-3-phenylpropanal. Moreover, 3-methyl butanal, which was also determined in the pastırma groups (Table 3), is a highly effective flavoring compound in meat products such as dry sausage and ham [42].
There was no statistical difference in terms of aromatic hydrocarbons between the control group and the groups containing S. xylosus 39, S. equorum 53, and S. vitulinus 75 strains (p > 0.05). The sources of aromatic hydrocarbons can vary considerably. In dry-cured meat products, among aromatic hydrocarbons, toluene has an important effect on aroma [1], and it can come from animal feed or may occur as a result of lipid degradation and amino acid catabolism [33].
No difference was determined between the groups in terms of the 2-pentylfuran (p > 0.05). This compound has also been detected in various dry-cured meat products [33,44]. As a terpene compound, only limonene was identified in this study. There is no statistical difference between the groups in terms of this compound (p > 0.05). Terpenes are related to spices, especially red pepper, and may also originate from herbs used in animal nutrition [39]. There was no statistical difference between the groups in terms of acetic acid (p > 0.05), which was the only compound identified as an acid (Table 3).
PCA was applied to evaluate the relationships between the groups (S. xylosus 39, S. equorum 53 and S. vitulinus 75) and volatile compounds ( Figure 2). The first two principal components explained 82% (53% for PC1 and 29% for PC2) (Figure 2). PC1 was positively related to the control, S. vitulinus 75, and S. equorum 53, while S. xylosus 39 showed a negative correlation with PC1. On the other hand, the control group separated the groups containing strains in PC2. S. xylosus 39, S. vitulinus 75, and S.equorum 53 groups located on the negative side of PC2, while the control group placed on the positive side of PC2. In addition, volatile compounds were usually collected around S. vitulinus 75 in the positive side of PC1 (Figure 2). S. vitulinus 75 generally showed a higher abundance in terms of volatile compounds. Statistically higher levels of benzaldehyde and 2-methyl-3-phenyl propanal were determined in this group compared to other groups (Table 3).  Table 3).

Conclusions
S. xylosus 39 and S. vitulinus 75 increased the redness (a* value) of pastırma. S. equorum 53, on the other hand, decreased the TBARS value, which is a measure of lipid oxidation, compared to the control group. In addition, all autochthonous strains used as starter cultures decreased the level of palmitic acid, which has an important part in the saturated fatty acids of pastırma. According to the PCA result, the fatty acid composition of S. xylosus 39 and S. vitulinus 75 showed greater similarity. On the other hand, the use of autochthonous strains in pastırma production led to a partial difference in the profile of volatile compounds, and the result of PCA showed that the formation of many volatile compounds was positively correlated with the presence of S. vitulinus 75.
Funding: This research received no external funding.

Data Availability Statement:
The data presented in this study are available on request from the corresponding author.  Table 3).

Conclusions
S. xylosus 39 and S. vitulinus 75 increased the redness (a* value) of pastırma. S. equorum 53, on the other hand, decreased the TBARS value, which is a measure of lipid oxidation, compared to the control group. In addition, all autochthonous strains used as starter cultures decreased the level of palmitic acid, which has an important part in the saturated fatty acids of pastırma. According to the PCA result, the fatty acid composition of S. xylosus 39 and S. vitulinus 75 showed greater similarity. On the other hand, the use of autochthonous strains in pastırma production led to a partial difference in the profile of volatile compounds, and the result of PCA showed that the formation of many volatile compounds was positively correlated with the presence of S. vitulinus 75.
Funding: This research received no external funding.

Data Availability Statement:
The data presented in this study are available on request from the corresponding author.