Nutritional Quality of Four Novel Porridge Products Blended with Edible Cricket (Scapsipedus icipe) Meal for Food

Currently, no data exist on the utilization of the newly described cricket species (Scapsipedus icipe) meal as additive in food products, though they have high protein (57%) with 88% total digestibility as well as a variety of essential amino acids. This article presents the first report on the effects of processing techniques and the inclusion of cricket meal (CM) on the nutrient and antinutrient properties of four porridge products compared to a popularly consumed commercial porridge flour (CPF). Porridge enriched with CM had significantly higher protein (2-folds), crude fat (3.4–4-folds), and energy (1.1–1.2-folds) levels than the CPF. Fermented cereal porridge fortified with CM had all three types of omega-3 fatty acids compared to the others. The vitamin content across the different porridge products varied considerably. Germinated cereal porridge with CM had significantly higher iron content (19.5 mg/100 g). Zinc levels ranged from 3.1–3.7 mg/100 g across the various treatments. Total flavonoid content varied significantly in the different porridge products. The phytic acid degradation in germinated and fermented porridge products with CM was 67% and 33%, respectively. Thus, the fortification of porridge products with cricket and indigenous vegetable grain powder could be considered an appropriate preventive approach against malnutrition and to reduce incidences in many low-and middle-income countries.


Introduction
The malnutrition of children in sub-Saharan African (SSA) countries is a serious health concern [1,2]. Protein-energy malnutrition (PEM) and deficiencies of micronutrients including iron, zinc, and vitamin A are the most common forms of malnutrition reported in these countries [3,4]. Kenya is amongst the 20 countries accounting for 80% of the world's malnourished children [5] where stunting, wasting, and underweight in children below five years have been estimated at 26%, 4%, and 11%, respectively [6]. Malnutrition in infancy and early childhood affects physical growth and cognitive behaviour, leading to delays in mental and motor development, as well as increased morbidity and mortality [7]. Low socio-economic status is considered the key underlying cause where children lack food or survive on diets of low nutritional quality [8].
prised the effects of toasting, boiling, and drying techniques on proximate composition and microbial quality of R. differens and other edible insects only. None of these studies looked at the potential effects of processing techniques and mixing of amaranth integrated with cricket meal on compounded human food products. This study sought to bridge this gap by comparing the effects of four common food processing methods of mixing of amaranth, cricket meal, and finger millets into porridge products on the nutrient (proximate composition, fatty acids, and minerals) and antinutrient composition as well as bioactive compounds (total flavonoid content and vitamins). This detailed report on the effect of the different processing methods applicable to cereal-based porridge food products with cricket meal is inevitable, especially with recent findings revealing the significance of edible insects and amaranth in the fight against malnutrition and food insecurity [9]. The campaign for consumption of insects, grains, and vegetables for the maintenance of good health has become ever more popular globally [29,30,39].

Raw Materials
Grain amaranth (Amaranthus cruentus) and finger millet (Eleusine coracan L.) were purchased from a local market in Nairobi, Kenya. One of the most traded brands of commercial porridge flour (CPF) (FAMiLA ® pure WIMBI porridge, Unga Limited, Nairobi, Kenya) was purchased from a supermarket in the Kenyan capital, Nairobi. The unique formulation of FAMiLA ® consists of pure finger-millet flour fortified with minerals (calcium), carbohydrates, and proteins (particularly lysine). This product was chosen because it is popular and consumed by adults and children of all ages. On the other hand, edible crickets (Scapsipedus icipe) were obtained from the Animal Rearing and Containment Unit (ARCU) at the International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya.

Preparation of Crickets
Frozen cricket samples were allowed to thaw overnight at a 5 • C refrigerated temperature. The samples were then washed thrice in fresh, clean tap water at 18 • C to remove dirt, drained, and appropriately heat-treated in hot water at 100 • C for 5 min for efficient sterilization. Thereafter, the crickets were oven-dried (WTB binder, Tuttlingen, Germany) at 60 • C for 24 h, milled using an electric grinder [Medical Research Council (MRC) laboratory grinder, London, UK], and sieved through a 0.595-mm aperture sieving mesh. The resulting powder was packaged in a sterile zip-lock polyethylene bag and stored at 4 • C for subsequent composite flour formulation.

Processing of Finger Millet and Amaranth Grains
Finger millet and amaranth grains were each divided into a batch of 500 g in four replications. The first batch was germinated according to the method described by Onyango et al. [40]. Briefly, the grains were cleaned and steeped in tap water for 24 h at 25 • C and germinated for 72 h at 25 • C in the dark, while being moistened and turned at 12-hour intervals. Germination was halted by oven-drying at 50 • C for 12 h. The dried germinated grains were then removed and milled using an electric grinder (MRC laboratory grinder, London, UK). The second batch was roasted according to the modified method [41]. The grains were spread in a uniform, thin layer on an oven tray and roasted in a pre-heated oven at 120 • C for 20 min. The grains were allowed to cool to room temperature before milling. The third batch was milled and fermented according to the method described by Onyango et al. [10] with slight modifications. The composite flour was mixed with tap water that had been boiled, then cooled to 45 • C, at a ratio of 2:3 (flour to water). The slurries were fermented spontaneously in round-bottomed flasks placed in a water bath (Blue M Electric Company, IL 60406, USA) at 45 • C. After 24 h the fermented slurries were inoculated into freshly prepared slurries before fermenting at 45 • C for 24 h. The samples were then spread on trays and dried in an oven at 50 • C for 24 h and ground in an electric grinder (MRC laboratory grinder, London, UK). The fourth batch was unprocessed grains treated according to farmers' field practices of open sun drying and storage, using appropriate technology to ensure availability throughout the year [42]. Thereafter, the grains were milled and prepared for further processing into porridge products. The fifth batch was considered as the "control treatment" and consisted of the CPF, which had undergone approved commercial standards of processing. All the samples were ensured to have a moisture content of <12.0%.

Porridge Flour Formulations
Composite flour formulations were developed and optimized using the Linear Programming of the Excel solver 2010 version, based on the minimum recommended dietary allowance (RDA) for protein (13 g), energy (500 kcal), calcium (500 mg), iron (7 mg), and zinc (4.1 mg) for children aged 1-3 years [11,12]. Porridge formulations were prepared by mixing flours of finger millet, amaranth, and cricket, at a ratio of 60:30:10. The porridge mix samples were coded as follows: commercial porridge flour (CPF), fermented finger millet − amaranth + cricket (FFM-AC), germinated finger millet − amaranth + cricket (GFM-AC), roasted finger millet − amaranth + cricket (RFM-AC), and unprocessed sundried finger millet − amaranth + cricket (UFM-AC). These new products supplemented with cricket meal and amaranth were compared with a commercial porridge product widely used by millions of households in East Africa.

Analysis of Proximate Composition
Proximate analysis for the flour was determined following the Association of Official Analytical Chemists (AOAC) methods [13] in three replications. Moisture content was determined in an air oven adjusted to 105 • C (method 925.10). The Kjeldahl method (method 978.04) was used to assess the crude protein (N × 6.25). Fat content was extracted using petroleum ether in a Soxhlet extractor (method 930.09). Ash content was determined by gravimetries (method 930.05). Fibre was determined by acid digestion and loss of ignition (method 930.10). Carbohydrate contents were determined as the difference (CHO% = [100−protein %−fat %−crude fibre %−ash %]). The energy value was computed by multiplying the Atwater factors of 4, 9, and 4 with protein %, fat % and carbohydrate % contents, respectively.

Determination of Mineral Composition
Mineral composition was established in three replications by the ICP OES quantitation method as follows. Exactly 0.5 g of each sample was digested with concentrated HNO 3 (8 mL) and 30% H 2 O 2 (2 mL) and left overnight in a fume chamber. Samples were then digested in a temperature-controlled block digester (Model TE007-A, TECNAL, São Paulo, SP, Brazil) following these conditions: 75 • C for 30 min, 120 • C for 20 min, 180 • C for 20 min, and 200 • C for 10 min. Resulting solutions were cooled and transferred to 25 mL falcon tubes and diluted with 2% nitric acid. Mineral compositions were assessed using an inductively coupled plasma optical emission spectrometer (ICP OES) (Model Optima 2100 DV Perkin Elmer, MA, USA) and analysed using WinLab 32 software (Perkin Elmer, Poway, CA 92064, USA). The following operational conditions were used: radiofrequency power (1.45 kW), auxiliary gas flow rate (1.5 L min −1 ), plasma gas flow rate (15.0 L min −1 ), nebuliser gas flow rate (0.7 L min −1 ), sample flow rate (1.5 L min −1 ), source equilibrium time (10 s), and delay time (10 s Oil extraction from the formulated porridge flours was achieved based on the modified method [43]. In three replications, 5 g each of sample were diluted with 10 mL of 2:1 dichloromethane (DCM) and methanol (MeOH). The mixtures were vortexed for 1 min, sonicated for 10 min, and centrifuged at 4200 rpm for 10 min. The supernatants were filtered using grade 1 and 90 mm (diameter) Whatman filter paper into a 250 mL round bottomed flask and evaporated in vacuo to yield approximately 200 mg of oil.

Fatty Acid Determination
Compositions of fatty acid (FA) in the oil extract from formulated porridge flours were examined as fatty acid methyl esters (FAMEs), according to the modified method [27]. Approximately 1 mL of sodium methoxide solution (100 mg/mL) was added to 100 mg of recovered oil extract, vortexed for 1 min, followed by sonication for 10 min. The resulting mixture was placed in a water bath (70 • C) for 1 h and the reaction halted by adding of 100 µL deionized water, followed by vortexing for another 1 min. To extract the FAMEs, 1 mL of gas chromatography (GC)-grade hexane (Sigma-Aldrich, St. Louis, MO, USA) was added to the mixture, followed by 20 min centrifugation at 14,000 rpm. The resulting hexane layer (upper) was dried over anhydrous Na 2 SO 4 , and analysis was performed using an Agilent GC-MS on a 7890A GC, connected to a 5975 C mass selective detector (Agilent Technologies Inc., Santa Clara, CA, USA). GC was fitted with a (5%-phenyl)-methylpolysiloxane (HP5 MS) low bleed capillary column (30 m × 0.25 mm i.d., 0.25 µm (J&W, Folsom, CA, USA). The sample injection volume was 1 µL. Helium acted as the carrier gas at flow rates of 1.25 mL/min. The oven temperature was programmed between 35 to 285 • C, with the initial and final temperature kept for 5 and 20.4 min, respectively, with a rising rate of 10 • C minute −1 . An ion trap mass selective detector was maintained at the ion source temperature of 230 • C and quad temperature of 180 • C. The mass detector was run in electron impact (EI) mode (70 eV). Fragment ions were determined in the full scan mode at over 40-550 m/z mass range. The filament was delayed for 3.3 min. Authentic standard methyl octadecenoate (0.2-125 ng/µL) serial dilutions prepared from octadecanoic acid (≥95% purity) (Sigma-Aldrich, St. Louis, MO, USA) were analysed using GC-MS in full scan mode. GC-MS generated a linear calibration graph of peak area versus concentration with the equation; [y = 5E + 0.7x + 2E + 0.7] with R 2 of 0.9997. This regression equation was used for the external quantification of the different FA and sterols from the samples.
A Hewlett-Packard (HP Z220 SFF intel xeon) workstation with ChemStation B.02.02. data acquisition software (Palo Alto, CA, USA) was used to control the operation of GC-MS. ChemStation integration parameters were calibrated as follows: initial threshold = 3, initial peak width = 0.010, initial area reject = 1, and shoulder detection = on. These calibrations yielded the mass spectrum for the peaks. The identification of compounds were based on the comparison of the generated mass spectra and retention times with those of authentic standards and reference spectra in library-MS databases: National Institute of Standards and Technology (NIST) 05, 08, and 11. The determination of the FAMES in all the flour samples was carried out in triplicates.

Determination of Water-Soluble Vitamins
The determination of water-soluble vitamins was carried out according to Thermo Fisher Scientific [28] (Waltham, MA, USA). For sample preparation, 100 mg of flour sample was mixed with 25 mL of distilled water in a 50 mL falcon tube. The mixture was ultrasonicated for 15 min and solution filtered through 0.2-µm filters into UPLC vials. The chromatographic analysis was performed on a Liquid chromatographic system with a diode array detector (LC-30AC with Nexera column oven CTO-30A, Shimadzu, Tokyo, Japan). A Phenomenex C18 Column Synergi, 100 × 3.00 mm, 2.6 µm polar (Phenomenex, Torrance, CA, USA) at 30 • C, was used. The mobile phase consisted of two phases: mobile phase A: 25 mM phosphate buffer; mobile phase B: 7:3 v/v Acetonitrile-Mobile phase A. The total run time was 12 min with a flow rate of 0.4 mL min −1 . Stock solutions of 1.0 mg/mL were prepared by dissolving the individual water-soluble vitamin standards in distilled water except for Vitamin B 2 (in 5 mM potassium hydroxide) and Vitamin B 9 (in 20 mM potassium hydrogen carbonate). Four calibration standards at a concentration of 2, 5, 10 and 15 µg/mL were prepared from the mixed stock solutions. The retention times (mins) for the vitamins were as follows: Vitamin C-1.596, Vitamin B 1 -1.922, Nicotinic acid-2.228, Vitamin B 6 -3.496, Nicotinamide-5.050, Vitamin B 5 -6.772, Vitamin B 9 -8.236, Vitamin B 12 -8.936, and Vitamin B 2 -9.224. R 2 was ≥0.996. All determinations were carried out in triplicates.

Determination of Fat-Soluble Vitamins
Fat-soluble vitamins was determined according to a method described by Bhatnagar et al. [29]. Briefly, 6 mL of ethanol with 0.1% (BHT)) was added to 500 mg of the flour sample and homogenized. To the resulting mixture, 120 µL of potassium hydroxide 80% (w/v) was added and vortexed for 1 min followed by incubation at 80 • C for 5 min. Cooling was carried out by placing the test tubes in ice, and 4 mL of deionized water was added to the mixture to enhance phase separation, followed by vortexing for 1 min. For extraction, 5 mL HPLC-grade hexane (Sigma-Aldrich, St. Louis, MO, USA) was added to the mixture, followed by a 5 min centrifugation at 3000 rpm. The resulting hexane layer (upper) was transferred into a separate test tube, and pellet was re-extracted twice more using hexane and the upper phases collected and pooled. Drying was carried out under nitrogen gas flow, and the residue reconstituted in 1 mL of methanol: tetrahydrofuran (85:15 v/v), vortexed and sonicated for 30 s and filtered into HPLC sample vials. Analysis was performed using reverse-phase HPLC (Shimadzu, Tokyo, Japan) linked to SPD-M2A detector. The UPLC was fitted with a YMC C30, carotenoid column (3 µm, 150X3.0 mm, YMC Wilmington, NC, USA). The mobile phase consisted of two phases: A: methanol/tert-butyl methyl ether/water (85:12:3, v/v/v, with 1.5% ammonium acetate in the water) and B: methanol/tert-butyl methyl ether/water (8:90:2, v/v/v, with 1% ammonium acetate in the water). The injection volume was 10 µL with a total flow rate of 0.4 mL/min. The retention times for retinol and αand γ-tocopherol were 2.74, 5.40, and 6.29 min, respectively. Compounds presenting the eluting sample were monitored at 290 nm. Peaks were identified by their retention time, and absorption spectra were compared to those of known standards (Sigma Chemicals). Sample concentrations were calculated by comparing peak area of samples to peak area of the standards.

Assessment of Phytic Acid, Tannins, and Flavonoids
Phytic acid contents were determined using a K-PHYT Phytic Acid (Phytate)/Total Phosphorus kit (Megazyme Int. Ireland Ltd., Wicklow, Ireland) following the manufacturer's instructions [30]. A flour sample (1 g) was added to 20 mL of 0.8 M HCl and mixed by shaking at room temperature overnight, and 1.5 mL of the resulting extract was centrifuged at 13,000 rpm for 10 min. The supernatant (0.5 mL) was neutralized with 0.5 mL of 0.8 M NaOH and used for the enzymatic dephosphorylation reaction and the subsequent total phosphorous and inorganic phosphate quantification. In parallel, 0.5 mL of the neutralized sample were used to quantify the inorganic phosphate of the sample. After the enzymatic treatment, the total phosphorous and total inorganic phosphate of the samples were used for colorimetric development to estimate the phosphorous content. This kit measures the inorganic phosphate released from the extracted flour sample after treatment with phytase and alkaline phosphatase. The free inorganic phosphate content was estimated from samples not treated with phytase. Experiments were performed in triplicate.
Total tannin content was determined by Folin-Denis method, using a microplate reader as outlined by Saxena et al. [31]. The results were expressed in mg tannic acid equivalents (TAE)/100 g of sample. The total flavonoid content was analysed using the Aluminium Chloride colorimetric [32]. The results were expressed in mg catechin equivalents (CEQ)/100 g of dry sample. All determinations were carried out in triplicates. A flowchart of the various processes involved throughout the development of the novel porridge products and nutrient profiling are summarized in Figure 1 below. ment with phytase and alkaline phosphatase. The free inorganic phosphate content was estimated from samples not treated with phytase. Experiments were performed in triplicate.
Total tannin content was determined by Folin-Denis method, using a microplate reader as outlined by Saxena et al. [31]. The results were expressed in mg tannic acid equivalents (TAE)/100 g of sample. The total flavonoid content was analysed using the Aluminium Chloride colorimetric [32]. The results were expressed in mg catechin equivalents (CEQ)/100 g of dry sample. All determinations were carried out in triplicates.
A flowchart of the various processes involved throughout the development of the novel porridge products and nutrient profiling are summarized in Figure 1 below.

Determination of Molar Ratios
Mineral bioavailability (zinc, iron, and calcium) was expressed as a phytate/mineral molar ratio [33]. Moles of phytic acid were calculated by dividing the recorded value of phytic acid with its atomic weight (660), while the moles of minerals (zinc, iron, and calcium) were determined by dividing the recorded values by the individual molecular weight of the respective compounds (i.e., Zn = 65, Fe = 56, and Ca = 40).

Statistical Analysis
Datasets of proximate compositions, mineral contents, fatty acids (FAs), and antinutritional data were subjected to analysis of variance (ANOVA). The means were separated using the HSD Tukey tests. Statistical analysis was performed using R Studio software version 1.3.1093-1(Boston, MA, USA) [34].

Proximate Composition
The results of the proximate composition and energy values of the formulated porridge products, which consisted of grain amaranth, finger-millet, and cricket, are presented in Table 1. Samples varied significantly (p < 0.05) in their proximate values, except on ash content. All products enriched with cricket had significantly (p < 0.05) higher protein (2-fold), fat (3.4-4-fold), and energy content (1.1-1.2-fold) when compared to the commercial porridge flour. The germinated sample presented the highest protein content (16.12 g/100 g) followed by the unprocessed sample (15.9 g/100 g), whereas the fat content of the fermented product (7.2 g/100 g) was significantly (p < 0.05) lower than that of other formulated products (8.1 g-8.31 g/100 g), but higher when compared to the commercial porridge flour (2.4 g/100 g). The highest crude fibre content was recorded in the germinated sample (5.3 mg/100 g), whereas the lowest was recorded in the fermented sample (3.3 mg/100 g).

Fatty Acids
The results of FAMEs in different porridge flour samples are presented in Table 2. Of the 44 FAs detected, the proportion of saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), and polyunsaturated fatty acids (PUFAs) present in porridge flour samples were 23%, 54%, and 23%, respectively. More FAMEs (3 to 4-fold) were detected in the cricket enriched porridge flours than in the commercial porridge flour. Additionally, the proportion of each group of FAMEs (SFA, MUFA, and PUFA) across the different porridge products are illustrated in Figure 2. Of the 24 SFAs detected, Methyl Hexadecanoate (palmitic acid) contributed the highest proportion, followed by Methyl Octadecanoate (stearic acid), across the flour samples. In addition, Methyl 9E-Octadecenoate (oleic acid) was the predominant MUFA, whereas Methyl 9Z,12Z-Octadecadienoate (linoleic acid, LA) accounted for the highest proportion of the PUFAS.    The proportion of omega-3 fatty acids, namely α -linolenic acid (ALA), Eicosapentaenoic acid (EPA), Alpha Eleostearic acid (α-ESA), and Docosapentaenoic acid (DHA) differed considerably across the flour samples. Α-linolenic acid (ALA) was detected in all the cricket enriched samples, while EPA was present in the fermented and germinated sample. DHA was only present in the fermented flour. On the effect of processing, PUFAs increased significantly (p < 0.05) by 30% during fermentation and decreased by 3% during roasting. Additionally, the roasting process caused a significant (p < 0.05) increase in both MUFAs and SFAs by 27% and 10%, respectively. Germination caused a slight increase in 9% and 12% in both MUFAs and PUFAs, respectively. The ratio of omega-6 to omega-3 varied significantly among the different flour samples and ranged from 3.2 to 4.4. The proportion of omega-3 fatty acids, namely α-linolenic acid (ALA), Eicosapentaenoic acid (EPA), Alpha Eleostearic acid (α-ESA), and Docosapentaenoic acid (DHA) differed considerably across the flour samples. α-linolenic acid (ALA) was detected in all the cricket enriched samples, while EPA was present in the fermented and germinated sample. DHA was only present in the fermented flour. On the effect of processing, PUFAs increased significantly (p < 0.05) by 30% during fermentation and decreased by 3% during roasting. Additionally, the roasting process caused a significant (p < 0.05) increase in both MUFAs and SFAs by 27% and 10%, respectively. Germination caused a slight increase in 9% and 12% in both MUFAs and PUFAs, respectively. The ratio of omega-6 to omega-3 varied significantly among the different flour samples and ranged from 3.2 to 4.4.

Vitamin Content
The results show significant variations in the vitamin content (Table 3). Cricket enriched formulations had a significantly (p < 0.05) higher content of vitamin B 12 , vitamin B 5 , B 6 , nicotinamide, and thiamine. Thiamine content ranged from 4.3-39.5 mg/100 g while vitamin B 12 was in the range of 3.2-37.7 mg/100 g. Processing methods had a significant (p < 0.05) effect on the vitamin levels. Germinated and fermented samples had enhanced levels of vitamin C, nicotinamide, vitamin B 5 , folate, and vitamin B 6 . However, there was a significant (p < 0.05) decrease to undetectable levels in the contents of nicotinic acid and thiamine during fermentation and germination, respectively. Vitamin B 12 showed significant reductions in all the processed porridge flours, while riboflavin content was not affected by processing. The roasting process had a negligible effect on vitamins, except for significant (p < 0.05) reductions in nicotinic acid and vitamin B 12, and a slight reduction in vitamin C. The retinol and αand γ-tocopherol content differed significantly (p < 0.05) in different porridge flour samples. Retinol content increased slightly during fermentation, while germination process decreased the levels of retinol and increased the α-tocopherol content. However, the roasting process did not affect the levels of αand γ-tocopherol.

Mineral Content and Molar Ratios
The mineral content varied significantly across the porridge flour products as shown in Table 4. The iron content of the formulated flours ranged between 8.59-19.48 mg/100 g with the germinated sample having the highest content (19.48 mg/100 g). Zinc content was in the range of 3.08-3.70 mg/100 g, while the range obtained for calcium was from 234.87 mg-278.61 mg/100 g. The calcium content of the commercial porridge flour was significantly (p < 0.05) higher at 312.69 mg/100 g when compared to the formulated samples, while the Zn content was significantly lower (1.86 mg/100 g). The fermented sample had significantly (p < 0.05) lower levels of magnesium and phosphorous, but the levels of calcium, copper, iron, and zinc did not vary when compared with the unprocessed formulation. Calcium and iron content in the germinated sample were higher than in other formulations. The roasting process did not significantly (p > 0.05) affect the mineral content. Processing methods had a significant (p < 0.05) influence on the phytate/mineral molar ratios ( Table 4). The critical values for the Phy:Fe, Phy:Zn, and Phy:Ca ratios were 1.0, 15.0 and 0.24, respectively [35]. Germinated sample had the lowest ratios for Phy:Fe (1.31) and Phy:Zn (7.43), whereas commercial flour had the lowest ratio for Phy:Ca (0.03). Phy:Ca ratios recorded in all the flours were below the limit threshold, except for the roasted sample (0.24) which had the highest values of all molar ratios.

Phytic Acid, Tannins, and Flavonoids
The phytic acid, tannin, and flavonoid contents of the flour samples are summarized in Figure 3. The phytic acid content of the roasted sample was significantly (p < 0.05) higher (1029 mg/100 g), followed by the unprocessed sample (837 mg/100 g), while the germinated sample had the lowest (279 mg/100 g) among the processed formulations. The highest (p < 0.05) tannin content (683 mg/100 g) was recorded in the fermented sample and the lowest content (381 mg/100 g) was detected in the germinated sample. The flavonoid content of the porridge flours was in the range of 60 mg-166 mg/100 g, with the highest value (166 mg/100 g) recorded for the commercial porridge flour.

Discussion
In low-and middle-income countries, child malnutrition contributes to about 45% of under-five child mortality, and this portends great danger to Africa's growth and development. One third of child deaths in Africa are attributable largely to protein energy malnutrition and micronutrient deficiencies [44,45], which could be solved by exploring underutilized nutritious crop and animal species of African origin. Traditional cereal-based porridge preparation has been considered as one of the major causes of protein energy malnutrition in developing countries [35]. The traditional complementary foods are usually low in protein and energy density, while containing high anti-nutrient content [36]. This study shows that formulating porridge products with underutilized edible cricket and amaranth meal, using household techniques to increase the protein, energy, vitamin, fatty acids, and mineral content in the porridge flour, could be the best substitute for traditional weaning flours. The recommended daily intake of protein in children aged 1-3 years is 13 g/day [12], and our study showed that the protein content in cricket and amaranth enriched flour ranged between 15.34-16.12 g/100 g (dwb). The observed protein content in cricket and amaranth enriched porridge flour is higher than that reported by Agbemafle et al. [46] in complementary food with orange-fleshed sweet potato, enriched with palm weevil larvae. The high protein in our formulated porridge products may be accredited to the addition of cricket and amaranth powder, which are known to be highly rich in protein [47].
The crude protein of the various porridge products fortified with cricket and amaranth meal varied slightly. This variation could be associated with the amino acids being metabolized into ammonia and other volatile flavour compounds during the various process methods, especially during fermentation [48]. A similar trend was reported during the fermentation of pearl millet by Osman [49]. Conversely, there was a slight increase in

Discussion
In low-and middle-income countries, child malnutrition contributes to about 45% of under-five child mortality, and this portends great danger to Africa's growth and development. One third of child deaths in Africa are attributable largely to protein energy malnutrition and micronutrient deficiencies [44,45], which could be solved by exploring underutilized nutritious crop and animal species of African origin. Traditional cereal-based porridge preparation has been considered as one of the major causes of protein energy malnutrition in developing countries [35]. The traditional complementary foods are usually low in protein and energy density, while containing high anti-nutrient content [36]. This study shows that formulating porridge products with underutilized edible cricket and amaranth meal, using household techniques to increase the protein, energy, vitamin, fatty acids, and mineral content in the porridge flour, could be the best substitute for traditional weaning flours. The recommended daily intake of protein in children aged 1-3 years is 13 g/day [12], and our study showed that the protein content in cricket and amaranth enriched flour ranged between 15.34-16.12 g/100 g (dwb). The observed protein content in cricket and amaranth enriched porridge flour is higher than that reported by Agbemafle et al. [46] in complementary food with orange-fleshed sweet potato, enriched with palm weevil larvae. The high protein in our formulated porridge products may be accredited to the addition of cricket and amaranth powder, which are known to be highly rich in protein [47].
The crude protein of the various porridge products fortified with cricket and amaranth meal varied slightly. This variation could be associated with the amino acids being metabolized into ammonia and other volatile flavour compounds during the various process methods, especially during fermentation [48]. A similar trend was reported during the fermentation of pearl millet by Osman [49]. Conversely, there was a slight increase in the protein content of the germinated product, which could be attributed to the mobilization of storage nitrogen and the synthesis of enzymatic proteins by the sprouting seeds during germination [50].
Fat content is an important factor in influencing the energy density of foods. It also provides essential FAs, improves the absorption of fat-soluble vitamins, and the sensory quality of food [51]. The fat content of formulated porridge products with cricket and amaranth meal was higher than that of commercial porridge products. Fermentation process caused a 13% reduction in fat content, indicating a possible active utilization of fats by microorganisms. These findings are in agreement with the observations by Assohoun et al. [52] who reported a reduction in fat content in fermented maize porridge products. The fiber content was increased during germination but reduced during fermentation and roasting. The increase in fibre content during germination could be attributed to the utilization of sugars by spouting seeds [53]. According to the Codex Alumentarius [54], the energy requirement for complementary foods is 400 kcal/100 g on dry weight basis. The energy contents in all the processed and formulated porridge products ranged from 408.12 to 413.92 kcal/100 g, which is higher compared to the commercial porridge flour (381.28 kcal/100 g). This implies that the new porridge products can sufficiently and adequately meet everyone's recommended energy allowance and nutrient needs.
Fatty acids, especially PUFAs, have enormous health benefits, including preventing degenerative ailments and promoting brain development and proper immune functioning [55,56]. The intake of omega-3 FAs during pregnancy and lactation reduces mortality and allergies in infants and improves their cognitive functions [57,58]. The formulations varied in the composition of FA with the fermented porridge products integrated with cricket and amaranth meal having the highest proportion of PUFAs, including the omega-3 FAs. The ratios of omega-6 to omega-3 FAs were lower than those reported by Paucean et al. [59] on wheat-lentil composite flour and Cheseto et al. [27] on insect oils and cookies. The fermentation process caused an increase in the levels of PUFAs and MUFAs and a decrease in the SFAs. Two omega-3 FAs (ALA + EPA) were detected in all the newly formulated flour products, but DHA was only present in the fermented porridge product, an indication of biosynthesis of DHA during fermentation. The increase in PUFAs, particularly omega-3 FAs, during fermentation could be attributed to microbial synthesis [60]. In this study, roasting increased the amounts of SFAs and oleic acid but decreased the amounts of unsaturated linoleic acid and omega-3 FAs. Studies indicate that the rate of FA oxidation increases as the number of double bonds increases, hence PUFAs will isomerize at a higher rate than MUFAs (oleic acid) [61], which partly explains the increase and decrease of oleic and linoleic acids, respectively, during roasting. Germination caused a 9% decrease in MUFAs and 12% increase in the levels of PUFAs. The resulted in an increase in PUFAs during germination, which is similar to the findings reported by Mariod et al. [62] on germinated black cumin seeds.
The formulated porridge products had increased amounts of essential minerals including zinc and iron. The iron content in the formulated products (8.6-19.5 mg/100 g) met the 7 mg/day recommended daily allowance (RDA) for iron in young children aged 1-3 years. Zinc content was in the range of 3.1-3.7 mg/100 g, which contributes about 75-90% RDA for zinc (4.1 mg/day) in young children. All formulated porridge products were high in calcium content (234.9-312.8 mg/100 g), although the levels were inadequate for providing the RDA (500 mg/day) for all the target age groups [11]. Fermentation processing only reduced the concentrations of Mg and P, while increasing that of Mn. reduction in mineral content during fermentation, has also been observed in previous studies involving cowpea porridge flour [63]. However, germination has been reported to increase the levels of Ca and Fe, which is consistent with previously reported studies by Tizazu et al. [64]. The increase in mineral content could be attributed to losses of water-soluble compounds during soaking.
Vitamins are organic compounds that are required in small amounts for the maintenance of normal health and biological reactions in the cells [65]. In this study, the fermentation process caused an increase in vitamin C, thiamine (B 1 ), pyridoxine (B 6 ), pan-tothenic acid (B 5 ), folate (B 9 ), and nicotinamide. The increase in water-soluble vitamins, particularly of the B-group, during fermentation could be as a result of microbial synthesis [66]. The results are in agreement with Kaprasob et al. [67] who reported an increase in B-group vitamins during fermentation of cashew apples, using probiotic strains of lactic acid bacteria (LAB). However, fermentation reduced nicotinic acid and vitamin B 12 in the various porridge products, and the losses might be attributed to utilization by LAB in their metabolic biosynthesis necessary for growth [68]. Germination on the other hand, enhanced the concentrations of vitamin C, nicotinic acid, vitamin B 6 , B 5, and B 9 . The increase could be due to the synthesis of these vitamins by the germinating seeds [69]. The increase in vitamin C during germination has been reported by other researchers and is attributed to the enzymatic hydrolysis of starch by amylases, leading to an increase in the bioavailability of glucose for biosynthesis of vitamin C [69,70]. However, the loss of thiamine to undetectable levels was recorded in the germinated porridge products; this might be due to leaching in the spouting medium [71]. The slight effect of roasting on riboflavin and nicotinamide in porridge flours suggests high thermal stability of B group vitamins [72]. The germination process increased the α-tocopherol content, and these results are comparable to those reported by Young et al. [73] on germinated rough rice seeds. The roasting process, however, did not affect the levels of tocopherols; this could be due to the fact that tocopherols are resistant to thermal degradation [74]. Contrarily, Stuetz et al. [75] reported the loss of tocopherols in roasted nuts compared to raw nuts; this variation might be explainable by the different roasting conditions used in the study.
Phytic acid is the principal storage form of phosphorous in plant seeds [76] and is known to reduce the bioavailability of dietary Zn, Fe, and Ca in humans and monogastric animals, owing to their chelating properties [77]. Both fermented and germinated porridge products had reduced levels of phytic acid when compared to the unprocessed sample. The reduction in phytic acid in the fermented product may be attributed to the effect of microbial phytase and the low pH which favours the activity of endogenous cereal phytase during fermentation [78]. Phytic acid degradation during the germination process could be due to increased activity of enzyme phytase in spouting grains which hydrolyse phytic acid into lower inositol phosphates [79]. Germination decreased phytic acid by 67% as compared to fermentation at 33%. The effect of germination on phytic acid levels could be attributed to a combination of processes such as phytase activity and the leaching of phytate ions into water during soaking before the germination of grains. Minor degradation of phytic acid during fermentation could also be attributed to the high phytic acid content and presence of endogenous phytase inhibitory compounds, such as tannins in both finger millet and amaranth grains [80]. Fermentation is more effective when carried out in grains with low anti-nutrients levels [81]. Contrarily, the increase in phytic acid during roasting could be as a result of an increase in lower phosphorylated inositol phosphates, which in turn increased the total phytic acid content. Similar results were observed by Frontela et al. [82]. However, our study did not differentiate between the classes of phytates but rather focused on the total phytic acid content. Moreover, the roasting temperature (120 • C) restricted endogenous phytase enzyme activity whose optimum temperature varies from 35 to 80 • C [83]. The low levels of phytic acid in the commercial porridge flour could be due to the use of exogenous phytate degrading enzymes during the industrial processing of the flour. The complete degradation of phytic acid in the four formulated porridge products is feasible with the use of additional exogenous phytases [83].
The phytate/mineral molar ratio is an indicator of mineral bioavailability in plantbased foods [33]. Flour samples from germinated grains had good bioavailability of Zn, while all porridge products, except for the roasted sample, had good bioavailability of Ca. The increased bioavailability of minerals corresponds with the reduction in phytic acid. The commercial porridge flour had good bioavailability of minerals except for Fe, and this could be attributed to low levels of phytic acid as well as the addition of extra minerals during fortification (as stated in the label).
Flavonoids exhibit powerful antioxidative properties, which makes them significant in the prevention of degenerative diseases [84]. The total flavonoid contents in our products ranged between 59.8-165.5 mg/100 g. Flavonoid content increased during fermentation, and this is consistent with the observations by Adetuyi and Ibrahim [85], who reported an increase in total flavonoid content during the fermentation of okra seeds. The increase in flavonoid content could be attributed to the release of simple phenolic compounds during the acid and microbial hydrolysis of complex phenolic compounds during fermentation [86]. A reduction in flavonoid content was noted during germination (42%) and roasting (10%). The reduction during roasting could be due to flavonoids' sensitivity to high temperatures [87]. The results on the reduction of flavonoids during roasting are similar to the findings of other authors [88].
Tannins are regarded as antinutrients because of their ability to inhibit digestive enzymes, lowering the digestibility of most nutrients, particularly proteins [89]. Tannin content reduced during germination is probably due to the complexing of tannins with seed proteins and metabolic enzymes [90] and leaching into the sprouting medium [91]. Significant increase in tannin content observed during fermentation might be due to the hydrolysis of various components, including condensed tannins by catabolic enzymes. Similar findings were reported by Osman [49] during the fermentation of pearl millet. However, reductions in tannin content during fermentation have also been reported [92].

Conclusions
The projected growth in global population with the attendant increase in food demands, especially in the African region, calls for focusing and devoting more attention to the underutilized crop and animal species, which have great potential to influence and improve food security for African nations and promote sustainable rural growth and development. Grain amaranth and edible insects are some of such neglected and underutilized species, despite their great inherent health-promoting components, which are good for human applications and uses. The demand for indigenous vegetable/grains and cricket-based products have risen dramatically in recent years because of the rapidly expanding human population. Our findings have also demonstrated the importance of integrating cricket and amaranth grain meal into porridge products to produce nutritious food sufficient for meeting the recommended daily allowance of the most vulnerable segments of the population, if processed properly. The comparative analysis of various processing protocols would empower communities to develop acceptable composite porridge products that are appealing to the consumers and increase demand. The four porridge products developed in this study are higher in energy density and nutritional value, as evidenced by the improved fat, protein, vitamin, and mineral contents in the various products, compared to the widely consumed commercial porridge product. Although complete degradation of phytic acid was not achieved, this study demonstrated that germination and fermentation techniques played a key role in improving the bioavailability of nutrients in the porridge products. Additional, innovative traditional processing technologies to increase phytic acid degradation are warranted. From a nutritional point of view, further studies on protein digestibility and utilization, the bioavailability of essential mineral and trace elements as well as other factors such as pH, the concentration of enhancers, and inhibitors (dietary fibre and polyphenols) would be crucial, particularly for cricket-based food products.