Effect of Phenolic Extract from Red Beans (Phaseolus vulgaris L.) on T-2 Toxin-Induced Cytotoxicity in HepG2 Cells

Red beans contain human bioactive compounds such as polyphenols. Several in vitro studies have proposed the natural compounds as an innovative strategy to modify the toxic effects produced by mycotoxins. Hence, in this work, a complete investigation of the polyphenolic fraction of red beans was performed using a Q-Orbitrap high-resolution mass spectrometry analysis. Notably, epicatechin and delphinidin were the most detected polyphenols found in red bean extracts (3.297 and 3.108 mg/Kg, respectively). Moreover, the red bean extract was evaluated against the T-2 toxin (T-2) induced cytotoxicity in hepatocarcinoma cells (HepG2) by direct treatment, simultaneous treatment, and pre-treatment assays. These data showed that T-2 affected the cell viability in a dose-dependent manner, as well as observing a cytotoxic effect and a significant increase in ROS production at 30 nM. The simultaneous treatment and the pre-treatment of HepG2 cells with red bean extract was not able to modify the cytotoxic T-2 effect. However, the simultaneous treatment of T-2 at 7.5 nM with the red bean extract showed a significant decrease in ROS production, with respect to the control. These results suggest that the red bean extract could modulate oxidative stress on HepG2 cells.


Introduction
Red beans (Phaseolus vulgaris L.), in addition to being a great source of vegetable protein, fiber, and certain micronutrients in the human diet, contain a great variety of bioactive compounds. Bioactive compounds are simple substances that have biological activity, associated with their ability to modulate one or more metabolic processes, which results in the promotion of better health conditions. Different bioactive compounds have been studied for their positive effect on human health, such as the following: enzymes, probiotics, prebiotics, fibers, phytosterols, peptides, proteins, saponins, unsatured fatty acids, and phenolic compounds, among others [1]. Particularly, colored beans have significant amounts of phenolic compounds. Phenolic compounds are classified as flavonoids (flavones, flavonols, flavanones, isoflavones, anthocyanins, chalcones, dihydrochalcones, and catechins), phenolic acids (hydroxybenzoic, hydroxyphenyl acetic, hydroxyphenyl pentanoic, and cinnamic hydroxyl acids), tannins, stilbenes, and lignans. Phenolic acids, flavonoids, and anthocyanidins are the main phenolic compounds identified and characterized in beans [2,3]. Phenolic compounds determine the color of the seeds of these legumes; hence, in general, a higher phenolic content is observed in more pigmented beans [4]. Phenolic compounds, besides from contributing to the smell, taste, and color of food, have a long-term intake that could play a bioactive role due to their antioxidant activity, p-cumaric acid, apigenin 7-glucoside, genistein, delphinidin, naringin, cyanidin, rosmarinic acid, myricitrin, diosmin, isoquercetin, rutin, kaempferol 3-glucoside, vitexin, ellagic acid, luteolin 7-glucoside, myricetin, diadzein, quercetin, delphinidin 3,5-diglucoside, naringenin, luteolin, kaempferol, and apigenin, were acquired from Sigma-Aldrich (Milan, Italy). Stock solutions of T-2 were prepared in MeOH at appropriate working concentrations and maintained in the dark at −20 • C.

Preparation of Red Bean Extract
The polyphenols were extracted from red beans according to the procedure reported in the literature with some modifications [17]. In summary, 0.5 g of ground beans was extracted with 10 mL of a solution MeOH:H 2 O. The assayed mixtures were 20:80, 30:70, 50:50, 70:30, and 80:20 (v/v). Hydrochloric acid 2N was added to the sample until a pH adjustment to 2, to avoid the protonated forms of carboxylic groups present in polyphenols. Then, the mixture was subjected to a horizontal shaker (250 rpm) at room temperature for 3 h and centrifuged for 3 min at 3500 rpm. Finally, MeOH was evaporated from the acidified sample extract under reduced pressure (250 mbar) at 50 • C for 15 min by Buchi Rotavapor R-200 (Buchi, Postfach, Switzerland) and the aqueous extract containing polyphenols was filtered with a 0.2 µm polytetrafluoroethylene (PTFE) filter and stored in amber glass flask at 4 • C.

Determination of Total Phenolic Content (TPC)
The Folin-Ciocalteu assay was used to determine the total phenolic content in accordance with the procedure reported by Izzo et al. [18]. Briefly, 0.5 mL of red bean extract or blank (deionized water) was diluted with deionized water (4.5 mL) and 0.25 mL of Folin-Ciocalteu reagent 1 N was added. Then, 1 mL of 2% sodium carbonate solution was added and the mixture was allowed to stand at room temperature for 1 h in dark conditions. Finally, the absorbance was measured with a spectrophotometer at 765 nm against a reagent blank. The analysis was carried out in triplicate and the results were expressed as mg of gallic acid equivalents (GAE) per Kg of sample.

Determination of Polyphenolic Profile
The polyphenolic profile of the red bean extracts was carried out on a UHPLC-Q Exactive Orbitrap-HRMS system (Thermo Fisher Scientific, Waltham, MA, USA), composed of a Dionex Ultimate 3000 liquid chromatograph equipped with a solvent rack compartment (SRD-3x00), a quaternary rapid separation pump (LPG-3400RS), a rapid separation autosampler (WPS-3000RS), and a temperature-controlled column compartment (TCC-3000SD). The chromatographic separation was performed on a Kinetex F5 (50 × 2.1 mm; 1.7 µm) reverse-phase column (Phenomenex, Milan, Italy) at 25 • C. The mobile phase consisted of water containing 0.1% formic acid (A) and MeOH containing 0.1% formic acid (B). The separation gradient consisted of an initial 0% of phase B, increasing to 40% B in 1 min, 80% B in a further 1 min, and 100% B in 3 min. Then, the gradient was held at 100% B for 4 min and reduced to 0% B in 2 min, followed by 2 min of column re-equilibration at 0% B. The total run time was 13 min. The flow rate was 0.5 mL/min and the injection volume was 1 µL.
The mass spectrometer was equipped with an electrospray (ESI) source that simultaneously operates in positive and negative ion switching mode. Full ion MS and all ion fragmentation (AIF) were set as scan events. The following settings were used in full MS mode: resolution power of 70,000 Full Width at Half Maximum (FWHM) (defined for m/z 200); automatic gain control (AGC) target: 1 × 10 6 ; scan range: 80-1200 m/z; injection time set to 200 ms; scan rate set at 2 scan/s. The ion source parameters were as follows: sheath gas pressure: 18; auxiliary gas: 3; spray voltage: 3.5 kV; capillary temperature: 320 • C; S-lens RF level: 60; auxiliary gas heater temperature: 350 • C. For the scan event of AIF, the parameters in the negative and positive mode were set as follows: mass resolving power = 17,500 FWHM; ACG target = 1 × 10 5 ; maximum injection time = 200 ms; scan Foods 2022, 11, 1033 4 of 15 time = 0.10 s; scan range = 80-1200 m/z; isolation window to 5.0 m/z; retention time to 30 s. The collision energy was varied between 10 and 60 eV to acquire representative product ion spectra.
For the identification and confirmation of the molecular ion and fragments, a mass tolerance below 5 ppm was set. Data analysis and processing were performed using Xcalibur software, v. 3.1.66.10 (Xcalibur, Thermo Fisher Scientific, Waltham, MA, USA).

Determination of Antiradical Activity (DPPH)
The total free radical scavenging activity of the red bean extracts was determined using the method reported in the literature with modifications [19]. Briefly, DPPH (4 mg) was solubilized in 10 mL of MeOH and then diluted to reach an absorbance value of 0.90 (±0.05) at 517 nm. This solution was used to perform the assay and 200 µL of red bean extract was added to 1 mL of working solution. The mixture was vortexed, kept for 90 min in the dark, and centrifuged for 5 min at 11,000 rpm. Finally, the decreased absorbance was measured at 517 nm. The analysis was carried out in triplicate and the results were expressed as mmol Trolox Equivalents (TE) per Kg of sample.

Cell Culture
Human hepatocarcinoma (HepG2) cells (ATCC: HB-8065) were cultured in DMEM medium supplemented with 10% NBCS, 100 U/mL penicillin, and 100 mg/mL streptomycin. The cells were maintained at pH 7.4, 5% CO 2 at 37 • C, and 95% air atmosphere at constant humidity. The cells were subcultured routinely twice a week with only a small number of sub-passages (<20 passages) in order to maintain genetic homogeneity. HepG2 cells were subcultured after trypsinization in a 1:3 split ratio. The medium was changed every 5 days. The final mycotoxin concentrations tested were achieved by adding T-2 mycotoxin to the culture medium, with a final MeOH concentration ≤ 1% (v/v).

HepG2 Cells Treatment
The HepG2 cells were cultured in 96-well tissue culture plates by adding 200 µL/well of density at 2 × 10 4 cells/well. After the cells reached 80% confluence, the culture medium was replaced with a fresh medium containing different concentrations of T-2 (7.5, 15, and 30 nM) and serial dilutions of red bean extracts (from 1:32 to 1) buffered to pH 7.4. Then, the plates were incubated in the dark at 37 • C and 5% CO 2 for 24 h. The assayed T-2 concentrations correspond to sublethal T-2 concentrations for HepG2 cells (<IC 50 ), based on previous studies carried out in our laboratory [20], and they were IC 50 /2, IC 50 /4, and IC 50 /8, respectively.
The following two more assays were performed: simultaneous treatment and pretreatment. On one hand, for pre-treatment studies, HepG2 cells were exposed to one red bean extract dilution according to previous cell proliferation assays (red bean extract 1:8 dilution) for 1 and 24 h. Then, the medium containing the red bean extract was removed and cells were exposed at the T-2 concentrations described above for 24 h. On the other hand, to conduct studies of simultaneous treatment, HepG2 cells were exposed to the assayed T-2 concentrations and the 1:8 diluted red bean extract for 24 h.
Appropriate controls containing the same amount of solvent were included in each experiment.

Determination of Cell Viability
Cell viability was determined in HepG2 cells by the MTT assay. The MTT assay is based on the capacity of viable cells to metabolize, via a mitochondrial-dependent reaction, specifically, the reduction of yellow tetrazolium salt to an insoluble purple formazan crystal. The MTT assay was carried out according to the procedure reported by Ruiz et al. [21]. In summary, after treatment studies, the medium containing the compounds was removed and each well received 200 µL of fresh medium containing 50 µL of MTT. The plates were returned to the incubator in the dark at 37 • C and 5% CO 2 for 3 h. Then, the MTT solution was removed and 200 µL of DMSO was added, followed by 25 µL of Sorensen's glycine buffer. The absorbance was measured at 620 nm on a Wallace Victor 2 , model 1420 multilabel counter (PerkinElmer, Turku, Finland). The blank absorbance value (from wells without cells but treated with MTT) was subtracted from all absorbance values.
Cell viability was expressed as a percentage relative to control cells (≤1% MeOH). Three independent experiments were conducted with eight replicates each, and the results were expressed as the mean ± standard error of the mean (SEM) of different independent experiments.

Determination of Reactive Oxygen Species (ROS)
Intracellular ROS production was monitored in HepG2 cells by adding H 2 -DCFDA [22]. H 2 -DCFDA is taken up by cells and then deacetylated by intracellular esterases; the resulting non-fluorescent 2 ,7 -dichlorodihydrofluorescein (H 2 -DCF) is converted to greatly fluorescent dichlorofluorescein (DCF) when oxidized by ROS. Briefly, 2 × 10 4 cells/well were seeded in a 96-well black polystyrene culture microplate. Once cells exhibited 80% confluence, the culture medium was replaced and cells were loaded with 20 µM H 2 -DCFDA in a fresh medium for 20 min in darkness. Then, H 2 -DCFDA was removed and replaced by a fresh medium containing 1:8 diluted red bean extract, T-2 (30, 15, and 7.5 nM), and the combination of diluted red bean extract with T-2 at the different concentrations assayed. Finally, the fluorescence emitted by the DCF was monitored at different times (0, 5, 15, 30, 45, 60, 90, and 120 min) on a Wallace Victor 2 , model 1420 multilabel counter (PerkinElmer, Turku, Finland), at excitation/emission wavelengths of 485/535 nm, respectively. The blank absorbance value (from wells without cells but treated with H 2 -DCFDA) was subtracted from all absorbance values.
The determinations were performed in three independent experiments with 24 replicates each and the results are expressed as an increase (%) in fluorescence in respect to control cells.

Statistical Analysis
Statistical analysis of data was carried out using the Statgraphics version 16.01.03 statistical package (IBM Corp., Armonk, NY, USA). Data are expressed as mean ± SEM of different independent experiments. The statistical analysis of the results was performed by Student's t-test for paired samples. The differences between groups were analyzed by employing one-way analysis of variance (ANOVA) continued by the Tukey HDS post-hoc test for multiple comparisons. Statistical significance was considered for p ≤ 0.05.

Total Phenolic Content and Antiradical Activity of Red Bean Extract
The extraction of phenolic compounds from red beans was optimized by testing different mixtures of MeOH:H 2 O and further determined through the Folin-Ciocalteu assay. The results are shown in Table 1. Based on the results obtained, the mixture 70:30 was optimal to extract phenolic compounds from red beans. The obtained TPC content (2325 ± 98 mg GAE/Kg) was in the same range to that of other studies performed on similar food matrices with values of 1230 mg/Kg [23], 1205 mg/Kg [24], 1690-4850 mg/Kg [25], and 3450 mg/Kg [26]. The TPC and DPPH data of undiluted red bean extracts are shown in Table S1. The total free radical scavenging activity of the red bean extract was evaluated through the DPPH assay. The results are in agreement with the previous assay being the highest antioxidant activity obtained with the mixture MeOH:H 2 O (70:30, v/v) ( Table 1). In addition, it was observed that the larger the phenolic content, the better the free radical scavenging activity. Significant differences in the phenolic content and the antioxidant activity amongst legume extracts were also reported by Zhao et al. [27]. Similarly, the values of antiradical activity obtained from red bean extract (49.2 ± 4.6 mmol TE/Kg) were comparable to those reported in other studies [28,29].
Besides the antioxidant properties shown by the phenolic compounds, the antiinflammatory activity by different mechanisms, including modulation of the inflammatory cascade, has also been reported in the literature [30]. García-Lafunete et al. reported that the phenolic rich extracts from beans inhibited the expression of IL-1β, IL-6, and TNF-α genes of stimulated macrophages RAW 246.7, with colored beans showing more activity than white beans [31].

Identification and Quantification of Active Compounds in Red Bean Extract
A total of 30 polyphenols were investigated in the red beans by using UHPLC-Q-Orbitrap HRMS analysis. The chromatographic and spectrometric parameters are shown in Table 2. The results showed a good chromatographic shape and separation of all studied compounds through the UHPLC gradient system employed within a 13 min run. Four different structural isomers, namely genistein and apigenin (m/z 269.0455), and vitexin and apigenin 7-glucoside (m/z 431.0983), were found. Compound identification was conducted by comparing the retention times of the standards with the peaks observed in sample extracts. For quantification purposes, calibration curves at eight concentration levels were built in triplicate. All regression coefficients were greater than 0.990.
The quantification of the main phenolic acids and flavonoids in red beans was performed by using a UHPLC-Q-Orbitrap HRMS method. The results are shown in Table 3. Up to 22 polyphenols were detected. Among the quantified polyphenols, epicatechin was the major flavanol (3.297 ± 0.119 mg/Kg). The delphinidin, an important anthocyanidin of pigmented beans, was quantified at 3.108 ± 0.023 mg/Kg. Flavanols and anthocyanidins represented 36% and 24.5% of total polyphenolic compounds in red bean samples, respectively. The other important polyphenols quantified in samples were p-coumaric acid, isoquercetin, and kaempferol 3-O-glucoside. The polyphenols content of undiluted red bean extracts are shown in Table S2.

Effects of Red Bean Extract on the Cell Viability by Individual Exposure
The HepG2 cell viability evaluated by the MTT assay after 24 h of exposure with red bean extract dilutions, from 1 to 1:32, is shown in Figure 1. The results clearly indicated that the viability of HepG2 cells was affected by the more concentrated red bean extracts. In particular, the undiluted (1) and diluted (1:2 and 1:4) red bean extracts significantly decreased HepG2 cell viability from 83% to 23%. Nonetheless, the red bean extract diluted 1:8 significantly increased the cell viability (20%) compared to the control.
The reduction in cell viability caused by the more concentrated or undilute has also been observed in other studies. Recently, Ziemlewska et al. [32] indi high concentrations of the bioactive compounds contained in red fruits inhibit cycle in the G2/M phase and caused cell death, exerting a negative impact on cel These authors demonstrated that high concentrations of phenolic extracts fro induced apoptosis due to the activation of the caspases.

Effects of Simultaneous Treatment in HepG2 Cell Viability Exposed to T-2 and Re Extract
The effect in HepG2 cells simultaneously exposed to T-2 (7.5, 15 and 30 nM diluted red bean extract is described in Figure 2. The HepG2 cell viability was a a concentration-dependent manner. Cytotoxic effects were observed in the cell to 30 nM T-2 alone and in combination with the extract, with a significant reduc viability compared to the control cells by 32% and 56%, respectively. This could the fact that the diluted red bean extract did not completely prevent T-2 cytoto diluted red bean extract slightly lowered HepG2 cell viability, which was a s significant decrease only in cells exposed to the highest T-2 concentration; whe not completely prevent T-2 cytotoxicity. On the other hand, cells exposed to the lowest T-2 concentration tested showed a significant increase in cell viability (11%) compared to the control; ce was slightly higher (13%) in cells simultaneously treated with T-2 and the diluted extract. The reduction in cell viability caused by the more concentrated or undiluted extracts has also been observed in other studies. Recently, Ziemlewska et al. [32] indicated that high concentrations of the bioactive compounds contained in red fruits inhibited the cell cycle in the G2/M phase and caused cell death, exerting a negative impact on cell viability. These authors demonstrated that high concentrations of phenolic extracts from berries induced apoptosis due to the activation of the caspases.

Effects of Simultaneous Treatment in HepG2 Cell Viability Exposed to T-2 and Red Bean Extract
The effect in HepG2 cells simultaneously exposed to T-2 (7.5, 15 and 30 nM) and 1:8 diluted red bean extract is described in Figure 2. The HepG2 cell viability was affected in a concentration-dependent manner. Cytotoxic effects were observed in the cells exposed to 30 nM T-2 alone and in combination with the extract, with a significant reduction in cell viability compared to the control cells by 32% and 56%, respectively. This could be due to the fact that the diluted red bean extract did not completely prevent T-2 cytotoxicity. The diluted red bean extract slightly lowered HepG2 cell viability, which was a statistically significant decrease only in cells exposed to the highest T-2 concentration; whereas it did not completely prevent T-2 cytotoxicity.

Effects of Pre-Treatment in HepG2 Cell Viability Exposed to T-2 and Red Bean Extract
The effects of cell pre-treatment with red bean extract for 1 h and 24 h before the T-2 addition are shown in Figure 3. The results indicated that the pre-treatment with red bean extract was not able to protect or ameliorate the cytotoxic T-2 effect in HepG2 cells in all T-2 concentrations tested. This effect was increased at 24 h of exposure. Similar results were reported by Kössler et al. [33], who showed that curcumin (phenolic compound) reduced cell viability and induced apoptosis in human embryonic kidney cells (HEK293) in a dose-dependent manner at 22 h of exposure. There are no other data from in vitro studies by other authors with bean extracts. However, Vila-Donat et al. [34] reported synergistic cytoprotective effects against cytotoxicity induced by alternariol on Caco-2 cells exposed to an extract obtained from other types of legumes, such as lentils. On the other hand, cells exposed to the lowest T-2 concentration tested (7.5 nM) showed a significant increase in cell viability (11%) compared to the control; cell viability was slightly higher (13%) in cells simultaneously treated with T-2 and the diluted red bean extract.

Effects of Pre-Treatment in HepG2 Cell Viability Exposed to T-2 and Red Bean Extract
The effects of cell pre-treatment with red bean extract for 1 h and 24 h before the T-2 addition are shown in Figure 3. The results indicated that the pre-treatment with red bean extract was not able to protect or ameliorate the cytotoxic T-2 effect in HepG2 cells in all T-2 concentrations tested. This effect was increased at 24 h of exposure. Similar results were reported by Kössler et al. [33], who showed that curcumin (phenolic compound) reduced cell viability and induced apoptosis in human embryonic kidney cells (HEK293) in a dose-dependent manner at 22 h of exposure. There are no other data from in vitro studies by other authors with bean extracts. However, Vila-Donat et al. [34] reported synergistic cytoprotective effects against cytotoxicity induced by alternariol on Caco-2 cells exposed to an extract obtained from other types of legumes, such as lentils.

Reactive Oxygen Species
The intracellular accumulation of ROS in HepG2 cells exposed to T-2 toxin was analyzed using the dichlorofluorescein assay (DCFH-DA). The cells were exposed to T-2 (7.5, 15, and 30 nM) and 1:8 diluted red bean extract for different exposure times (0, 5, 15, 30, 45, 60, 90, and 120 min), and the results are expressed as an increase in fluorescence (%) with respect to the control (Figure 4). The simultaneous exposure to T-2 at the lowest concentration tested (7.5 nM) and the red bean extract showed a significant reduction (p ≤ 0.05) in ROS production after 120 min of exposure with respect to control cells ( Figure 4A); whereas there were no significant differences in ROS production at 15 nM with respect to control cells ( Figure 4B). On the contrary, a significant increase (p ≤ 0.05) in ROS production with respect to controls was observed in the case of the simultaneous exposure to T-2 at 30 nM alone and in combination with the red bean extract at 60 min of exposure (Figure 4C).

Reactive Oxygen Species
The intracellular accumulation of ROS in HepG2 cells exposed to T-2 toxin was analyzed using the dichlorofluorescein assay (DCFH-DA). The cells were exposed to T-2 (7.5, 15, and 30 nM) and 1:8 diluted red bean extract for different exposure times (0, 5, 15, 30, 45, 60, 90, and 120 min), and the results are expressed as an increase in fluorescence (%) with respect to the control (Figure 4). The simultaneous exposure to T-2 at the lowest concentration tested (7.5 nM) and the red bean extract showed a significant reduction (p ≤ 0.05) in ROS production after 120 min of exposure with respect to control cells ( Figure 4A); whereas there were no significant differences in ROS production at 15 nM with respect to control cells ( Figure 4B). On the contrary, a significant increase (p ≤ 0.05) in ROS production with respect to controls was observed in the case of the simultaneous exposure to T-2 at 30 nM alone and in combination with the red bean extract at 60 min of exposure ( Figure 4C). Data reported by Lee et al. [34] demonstrated that the treatment of HepG2 cells with extracts obtained from different types of beans (black, red, and green) against the Tert Butyl Hydroperoxide (TBHP) agent significantly decreased the production of ROS. On the other hand, Yang et al. [3] showed that the level of intracellular ROS production in Data reported by Lee et al. [34] demonstrated that the treatment of HepG2 cells with extracts obtained from different types of beans (black, red, and green) against the Tert Butyl Hydroperoxide (TBHP) agent significantly decreased the production of ROS. On the other hand, Yang et al. [3] showed that the level of intracellular ROS production in HCT116 cells decreased significantly in cells treated with phenolic extracts of beans compared to untreated cells.
The abovementioned results can demonstrate the ability of phenolic extracts to modulate oxidative stress. However, further studies with regard to biological activity, including studies into the mechanisms of action and structure-activity relationships, are necessary to fully understand the modes of action of these bioactive compounds and to fully exploit their cytoprotective potential effect, as highlighted in the literature [35].

Conclusions
The chemical profile obtained from this study evidenced that red beans are a rich source of bioactive compounds such as flavanols and anthocyanidins, which confers antiradical activity and human health benefits. On the other hand, red bean extract (diluted 1:8) showed a significant increase in HepG2 cell proliferation after 24 h of exposure. Similar results were observed after HepG2 cell exposure to T-2 toxin at the lowest concentration assayed (7.5 nM) corresponding to its IC 50 /8, which could indicate a probable adaptative response of HepG2 cells. However, higher T-2 concentrations showed cytotoxic effects and ROS production on HepG2 cells. The results from the simultaneous and pre-treatment assays indicated that the 1:8 diluted red bean extract did not prevent T-2 cytotoxicity neither in simultaneous exposures nor with the pre-treatment. Finally, the combination of T-2 at 7.5 nM with the diluted red bean extract showed a decrease in ROS production compared to the control at the longest exposure time tested (120 min). The antioxidant activity or the possible T-2 hormetic effect observed in simultaneous treatment could be responsible for the latter result, suggesting that the red bean extract could modulate oxidative stress on HepG2 cells.

Supplementary Materials:
The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/foods11071033/s1, Table S1: Total phenolic content (TPC) and antiradical activity (DPPH) of undiluted red bean extracts using different mixtures of MeOH:H 2 O. Values are reported as mean ± SD of independent experiments performed in triplicate; Table S2. Polyphenols content in undiluted red bean extracts. Results are expressed as mean ± SD from three independent determinations.