Optimization of Osmotic Dehydration of Sapodilla (Achras zapota L.)

Sapodilla (Achras zapota L.) is a fruit with a great nutritional potential; however, its perishable nature is a great obstacle for commercialization/exportation. Herein, osmotic dehydration was applied to sapodilla to reduce post-harvest losses and obtain a stable product with acceptable sensorial characteristics. Initially, a 2³ full-factorial design was performed to determine the effect of temperature (30–50 °C), sucrose concentration (40–60% °Brix) and immersion time (90–240 min) on the moisture loss (ML), solid gain (SG) and dehydration efficiency index (DEI). The samples with higher DEI values were subjected to sensory analysis, followed by physicochemical, microbiological and structural analyses. The temperature and the concentration of the osmotic solution had significant influence (p < 0.05) on ML and SG, whereas DEI was significantly influenced (p < 0.05) by the concentration of osmotic solution and the immersion time. The sample produced by osmotic dehydration using the optimized conditions (40 °C, 50 °Brix; 165 min) obtained higher scores on the sensorial attributes, greater compliance with microbiological standards and generated turgor reduction and ruptures of sapodilla cell walls.


Introduction
Achras zapota L. (synonym of Manilkara zapota (L.) P.Royen) is a medium-sized evergreen species from the Sapotaceae family native to tropical areas of the Americas (from southern Mexico to parts of South America) and now also cultivated in other tropical regions throughout the world [1,2]. In Brazil, the tree is known as 'Sapotizeiro' and has good adaptation in the northeast region of Brazil due to appropriate soil and climate conditions [3,4]. The fruit is named as 'sapoti' in Brazil, and it is also known as 'chicozapote' or 'sapodilla' in other Latin countries. It has ovoid shape, brown bark and a soft pulp with sweet and slightly astringent taste [5]. The aroma of fresh fruit is typically minty, fatty/green and woody, containing at least 13 aroma-active compounds [6]. This fruit has been applied for the production of juices, wine, ice-creams, jam, cookies and other food formulations [5,[7][8][9].
The obtained data were adjusted to the following polynomial: where βn are the regression coefficients, Y is the response in question (ML, SG and ML/SG) and T, C and t are the independent variables (temperature, concentration of osmotic solution and immersion time, respectively). The responses of interest were thus defined: -Moisture Loss (ML)-calculated in percentage terms, based on the initial weight of the material: where ML(%) is the loss of moisture in relation to initial material, Mi = is the initial moisture content of matter (%), Uf is the final moisture content of matter (%), Mi is the total initial mass of matter (%), and Mf is the total final mass of matter.
-Solid Gain (SG)-calculated through a balance of mass acquired during the processing of solids: where SG (%) is the solid gain, • Brixi is the initial total soluble solids content of the material, • Brixf is the final content of total soluble solids of the material.
-Dehydration efficiency index (DEI): ML/SG The experimental design was built, and the data were analyzed by a Student's t-test through the statistical software Statistica 6.0 at the 5% level of significance using the StatSoft Inc., Statistica: Data Analysis Software System, Version 7 (2004).

Sensory Analysis
The assays with higher Dehydration Efficiency Indices (DEIs) were subjected to the acceptance analysis, using a structured nine-point hedonic scale (1 = very disagreeable, 9 = very much liked) to evaluate the attributes: aroma, color, flavor, texture and quality. The intention of purchase was evaluated by applying a 5-point hedonic scale which varied from 1 = "I would certainly not buy" to 5 = "I would certainly buy". Fifty tasters were randomly recruited among students and professors of the 'Departamento de Ciências Domésticas' (DCD/UFRPE; Recife, PE, Brazil), with the exclusion criterion of an aversion to sapodilla. Samples were randomly coded with three-digit numbers. The acceptability index (AI) for the overall quality attribute was calculated: AI(%) = Y × 100/Z (Y = average score obtained for the product; Z = maximum obtained score). This study was approved by the Research Ethics Committee, through the Brazil platform (Process: 331.686). The in natura Sapodilla and the sample that showed the best results in the sensory analysis were submitted to the analyses described in Sections 2.4.2-2.4.4. All analyses were performed in triplicate.

Centesimal Composition and Physicochemical Parameters
The following analyses were performed before and after osmotic dehydration of sapodilla: soluble solids by direct reading in an ATAGO manual refractometer expressed in • Brix; weight of the samples in a semi-analytical balance of the Tecnal brand; humidity, lipids, ashes, proteins, dietary fiber and pH (AOAC, 2002); carbohydrates [29]; total caloric value (TCV) by the calculation method; and water activity (Aw) in Decagon Aqualab 4 TE equipment.

Structural Analysis
The images were generated with a Scanning Electron Microscope (SEM; Quanta 200 FEG). In brief, the samples were fixed using 2.5% glutaraldehyde and in 0.1 M cacodylate buffer. Subsequently, the samples were washed in 0.1 M cacodylate buffer (three times). The tissue was post-fixed in 2% osmium tetroxide and 0.1 M cacodylate buffer. The dehydration process occurred using a graded acetone series (30%, 50%, 70%, 90% and three times at 100%; each procedure lasted 10 min). At the end of this stage, the samples were dried for 1 h and 10 min at 40 • C and 80 bar. The samples were then mounted on the stubs and sprayed with gold for 80 s. After this procedure, the samples were visualized in the SEM.

Analysis Statistics
All the experiments were carried out in triplicate and the results are expressed as mean values. A p-value < 0.05 was considered statistically significant. The data were analyzed using the statistical software Statistica 6.0.

Results and Discussion
In this study, a 2 3 factorial design approach was employed in order to optimize the process of osmotic dehydration of sapodilla. It was observed that the moisture loss (ML) was higher than the solid gain (SG) in all 17 assays ( Table 2). The differences between ML and SG are due to the larger size of sucrose molecules relative to water molecules, which allows water molecules to move faster than sucrose molecules [25]. Similar results were found when the osmotic dehydration was applied for apricot (Prunus armeniaca L.) [31], apple cv. "Idared" [32] and acerola (Malpighia punicifolia) [22]. These results confirmed the main aim of osmotic dehydration, which is to achieve maximum water loss with minimum solid gain.
A regression analysis was applied to model the ML, SG and DEI values as quadratic functions of the osmotic solution concentration, immersion time and temperature. The regression coefficients for the obtained models are presented in Table 3. The statistical analysis indicated that the adjusted models were considered predictive, not having a significant lack of adjustment and with R 2 values very satisfactory for all the answers. The R 2 values for ML, SG and DEI were 0.982, 0.979 and 0.947, respectively. Similar results were found when studying the mass transfer kinetics of radish cylinders (R 2 > 0.97) [33] and banana cylinders (R 2 > 0.98 for ML and SG) [26].   Figure 1 shows the influence of sucrose concentration and temperature on moisture loss. This verified that the interaction between the concentration of the osmotic solution and temperature induced significant effects on the ML levels. Higher moisture losses were obtained when the osmotic solution had concentrations higher than 50 • Brix and the operating temperatures were higher than 40 • C.
These results can be explained by the increase in the osmotic pressure gradient, as well as by the use of high temperatures which cause a decrease in the viscosity of the osmotic medium and lead to swelling, plasticization and destruction of the cell membrane structure, i.e., increased permeability of the membrane, which, in turn, favors water loss [22,26]. Similar results were obtained with the osmotic dehydration of apricots [31], where the use of higher concentrations of sucrose led to higher osmotic pressure gradients, resulting in higher solid gains and water loss over of the osmotic treatment period [21]. Figure 1 shows the influence of sucrose concentration and temperature on m loss. This verified that the interaction between the concentration of the osmotic s and temperature induced significant effects on the ML levels. Higher moisture losse obtained when the osmotic solution had concentrations higher than 50 °Brix a operating temperatures were higher than 40 °C. These results can be explained by the increase in the osmotic pressure grad well as by the use of high temperatures which cause a decrease in the viscosity osmotic medium and lead to swelling, plasticization and destruction of the cell mem structure, i.e., increased permeability of the membrane, which, in turn, favors wa [22,26]. Similar results were obtained with the osmotic dehydration of apricots [31], the use of higher concentrations of sucrose led to higher osmotic pressure gra resulting in higher solid gains and water loss over of the osmotic treatment period Sucrose solution was applied in the osmotic dehydration of fresh Terung (Solanum lasiocarpum Dunal), and verified that the increase in sucrose concentrati immersion time accelerated ML. The authors discussed that these results are due osmotic gradient between the hypertonic sucrose solution and the intracellular f the T. Asam slices [27]. This gradient promoted the diffusion of water from the sam the osmotic medium. Therefore, accelerated water removal can be achieved by incr the solution concentration to create a higher osmotic driving force. Similar result found in the osmotic dehydration of ripe papaya (Carica papaya L.) with various s concentrations (40, 50 and 60 °Brix), where greater ML and SG were archived highest sucrose concentrations [34]. Regarding temperature, similar results were ac in the osmotic dehydration of banana (Musa sapientum shum.) at temperatures from 55 °C, salt concentrations of 0-10 g/100 g and sucrose concentrations of 30-60 g/100 The interactions between temperature and time of immersion on SG is shown in 2 and Table 3. It was observed that these variables had significant influences on SG (p The highest levels of SG were obtained when the operating temperature was higher °C and immersion time was more than 210 min. Higher SG values were also achieved temperatures lower than 34 °C were combined with immersion times under 120 min. results were found in the process of the dehydration of apple cylinders using four d osmotic media (50% sucrose, 49.47% sucrose + 0.25% CaCl2, 48.27% sucrose + 0.5% and 40.52% sucrose + 0.50% CaCl2 + 2% NaCl) at temperatures of 20, 40 and 60 °C at 120 and 180 min intervals [35]. During the dehydration of T. Asam, it was also fou the SG was accelerated by the increase in temperature and immersion time, w maximum SG recorded at a temperature of 55 °C and immersion time of 210 min [27 Sucrose solution was applied in the osmotic dehydration of fresh Terung Asam (Solanum lasiocarpum Dunal), and verified that the increase in sucrose concentration and immersion time accelerated ML. The authors discussed that these results are due to the osmotic gradient between the hypertonic sucrose solution and the intracellular fluid of the T. Asam slices [27]. This gradient promoted the diffusion of water from the samples to the osmotic medium. Therefore, accelerated water removal can be achieved by increasing the solution concentration to create a higher osmotic driving force. Similar results were found in the osmotic dehydration of ripe papaya (Carica papaya L.) with various sucrose concentrations (40, 50 and 60 • Brix), where greater ML and SG were archived at the highest sucrose concentrations [34]. Regarding temperature, similar results were achieved in the osmotic dehydration of banana (Musa sapientum shum.) at temperatures from 25 to 55 • C, salt concentrations of 0-10 g/100 g and sucrose concentrations of 30-60 g/100 g [26].
The interactions between temperature and time of immersion on SG is shown in Figure 2 and Table 3. It was observed that these variables had significant influences on SG (p < 0.05). The highest levels of SG were obtained when the operating temperature was higher than 46 • C and immersion time was more than 210 min. Higher SG values were also achieved when temperatures lower than 34 • C were combined with immersion times under 120 min. Similar results were found in the process of the dehydration of apple cylinders using four different osmotic media (50% sucrose, 49.47% sucrose + 0.25% CaCl 2 , 48.27% sucrose + 0.5% CaCl 2 and 40.52% sucrose + 0.50% CaCl 2 + 2% NaCl) at temperatures of 20, 40 and 60 • C at 30, 60, 120 and 180 min intervals [35]. During the dehydration of T. Asam, it was also found that the SG was accelerated by the increase in temperature and immersion time, with the maximum SG recorded at a temperature of 55 • C and immersion time of 210 min [27].
The impact of temperature on moisture loss kinetics without any effect on solids gain is most obvious between 30 • C and 60 • C for vegetables and fruits [36]. It is reported that temperatures above 60 • C should be avoided because they reduce the quality of the final product, altering the structure of the cell membranes, resulting in a loss of selectivity, leading to greater incorporation of solutes in the fruit. Furthermore, elevated temperatures may induce significant changes in the texture and nutritional composition of the food as a consequence of nutrient losses from the product to the osmotic solution [26].  The impact of temperature on moisture loss kinetics without any effect on solids gain is most obvious between 30 °C and 60 °C for vegetables and fruits [36]. It is reported that temperatures above 60 °C should be avoided because they reduce the quality of the final product, altering the structure of the cell membranes, resulting in a loss of selectivity, leading to greater incorporation of solutes in the fruit. Furthermore, elevated temperatures may induce significant changes in the texture and nutritional composition of the food as a consequence of nutrient losses from the product to the osmotic solution [26].
The influence of temperature and concentration of the osmotic solution on SG was also found as significant (p < 0.05) (Figure 3). Higher temperature values (T > 46 °C) and lower solution concentration values (<44 °Brix) provided higher SG, whereas temperatures <34 °C and concentrations of solution of sucrose <44 °Brix resulted in a product with lower SG, thus leaving the final product closer to in natura conditions. The same behavior observed for sapodilla was observed for dehydrated radishes and pumpkins [37], i.e., the temperature and concentration of the osmotic solution influenced SG significantly (p < 0.05). The influence of temperature and concentration of the osmotic solution on SG was also found as significant (p < 0.05) (Figure 3). Higher temperature values (T > 46 • C) and lower solution concentration values (<44 • Brix) provided higher SG, whereas temperatures <34 • C and concentrations of solution of sucrose <44 • Brix resulted in a product with lower SG, thus leaving the final product closer to in natura conditions. The same behavior observed for sapodilla was observed for dehydrated radishes and pumpkins [37], i.e., the temperature and concentration of the osmotic solution influenced SG significantly (p < 0.05). The impact of temperature on moisture loss kinetics without any effect on solids gain is most obvious between 30 °C and 60 °C for vegetables and fruits [36]. It is reported that temperatures above 60 °C should be avoided because they reduce the quality of the final product, altering the structure of the cell membranes, resulting in a loss of selectivity, leading to greater incorporation of solutes in the fruit. Furthermore, elevated temperatures may induce significant changes in the texture and nutritional composition of the food as a consequence of nutrient losses from the product to the osmotic solution [26].
The influence of temperature and concentration of the osmotic solution on SG was also found as significant (p < 0.05) (Figure 3). Higher temperature values (T > 46 °C) and lower solution concentration values (<44 °Brix) provided higher SG, whereas temperatures <34 °C and concentrations of solution of sucrose <44 °Brix resulted in a product with lower SG, thus leaving the final product closer to in natura conditions. The same behavior observed for sapodilla was observed for dehydrated radishes and pumpkins [37], i.e., the temperature and concentration of the osmotic solution influenced SG significantly (p < 0.05). High SG values in the osmotic dehydration process are undesirable because they can lead to sensory changes in the product. Furthermore, when the concentration of solutes in plant tissues is high, the efficiency of dehydration is reduced due to the formation of an external barrier that hinders mass transfer [28].
In order to evaluate the influence of osmotic dehydration parameters on the ML and SG efficiency, the DEI values were determined. In Figure 4, it is possible to observe that the interaction between the sucrose osmotic solution concentration and the immersion time significantly influenced the DEI (p < 0.05). High values of this relationship are related to good dehydration conditions [37], because they reflect a treatment that provides high moisture loss and low solute absorption [28]. external barrier that hinders mass transfer [28].
In order to evaluate the influence of osmotic dehydration parameters on the ML and SG efficiency, the DEI values were determined. In Figure 4, it is possible to observe that the interaction between the sucrose osmotic solution concentration and the immersion time significantly influenced the DEI (p < 0.05). High values of this relationship are related to good dehydration conditions [37], because they reflect a treatment that provides high moisture loss and low solute absorption [28]. An increase in the concentration of the osmotic solution implies high osmotic pressure and, consequently, higher mass transfer rates [38]. Osmotic duration is another important variable that has an unavoidable influence on the mass transfer rate. It was noted that the prolonged immersion time improved the ML [27]. During the dehydrating cylinders of pumpkins, it was observed that the DEI increased with higher immersion time, temperature and, especially, concentration of the sucrose solution [37].
As presented in Figure 5, higher values of DEI were reached with values of temperature >48 °C and immersion times <120 min, and temperatures <40 °C and immersion times >165 min. These effects were observed in assays 7, 5 and 9, where the highest values of DEI were obtained ( Table 2). Similar results were seen in the osmotic dehydration process of T. Asam slices, where the optimal process conditions were predicted as a temperature of 38.1 °C, sucrose concentration of 55.6% and osmotic duration of 126.3 min [27]. An increase in the concentration of the osmotic solution implies high osmotic pressure and, consequently, higher mass transfer rates [38]. Osmotic duration is another important variable that has an unavoidable influence on the mass transfer rate. It was noted that the prolonged immersion time improved the ML [27]. During the dehydrating cylinders of pumpkins, it was observed that the DEI increased with higher immersion time, temperature and, especially, concentration of the sucrose solution [37].
As presented in Figure 5, higher values of DEI were reached with values of temperature >48 • C and immersion times <120 min, and temperatures <40 • C and immersion times >165 min. These effects were observed in assays 7, 5 and 9, where the highest values of DEI were obtained (Table 2). Similar results were seen in the osmotic dehydration process of T. Asam slices, where the optimal process conditions were predicted as a temperature of 38.1 • C, sucrose concentration of 55.6% and osmotic duration of 126.3 min [27]. Given the results, assays 7 and 9 obtained satisfactory ML and were chosen to carry out the sensory analysis (Table 2). Taking this criterion into account, assay 5 was eliminated.

Sensory Analysis
The means of acceptance scores are presented in Table 4. Assays 7 (34 °C, 56 °Brix, Given the results, assays 7 and 9 obtained satisfactory ML and were chosen to carry out the sensory analysis (Table 2). Taking this criterion into account, assay 5 was eliminated.

Sensory Analysis
The means of acceptance scores are presented in Table 4. Assays 7 (34 • C, 56 • Brix, 210 min) and 9 (40 • C, 50 • Brix, 165 min) did not differ statistically (p < 0.05) among the evaluated attributes, although assay 9 obtained higher averages (Table 4). In relation to the intention to buy, it was found that assay 9 also obtained a higher percentage of "certainly buy" (32%), whereas less individuals chose this for assay 7 (22%) ( Table 5). Regarding the acceptability index for the overall quality attribute, similar results were found for both samples tested: 71.55% (assay 7) and 75.11% (assay 9). These values (>70%) are considered as sensorially acceptable products [39]. The different conditions of the osmotic dehydration process had a notable influence on the acceptability of the final product. In view of the results of the acceptability index and purchase intention, assay 9 was selected for microbiological, physicochemical and structural analyses. Table 4. Mean acceptance scores for dehydrated sapodilla (assays 7 and 9).

Assay
Aroma Color Taste  Texture  Global Quality   7 6.50 ± 1.25 a 6.30 ± 1.47 a 6.64 ± 1.34 a 6.42 ± 1.54 a 6.44 ± 1.31 a 9 6.72 ± 1.41 a 6.58 ± 1.44 a 6.78 ± 1.58 a 6.82 ± 1.69 a 6.76 ± 1.39 a In each column, means followed by the same superscript letter ( a ) do not differ significantly at the 5% level of significance by the Student's t-test.

Microbiological Analysis
The dehydrated sapodilla (40 • C, 50 • Brix, 165 min) presented microbiological standards that met those established by Resolution RDC No. 12 of 2 January 2001, of the 'Agência Nacional de Vigilância Sanitária' [40] (Table 6), thus demonstrating good manufacturing practices.  Table 7 shows the centesimal composition of the sapodilla in natura and osmotically dehydrated. Sapodilla in natura has a similar profile to most fruits, exhibiting a high content of moisture and carbohydrates, and low contents of proteins and lipids. The contents of carbohydrates and proteins were similar (20.40 g/100 g and 0.38 g/100 g, respectively) to those values previously reported by Gonsalves [39] (20 g/100 g and 0.4 g/100 g, respectively) However, the total caloric value was higher (93.70 g/100 g) than that found by Gonsalves [39] (83 g/110 g). All parameters of the centesimal composition differed significantly (p < 0.05) between sapodilla in natura and dehydrated, except for ash content (Table 7). In addition, the ash content of the sapodilla in natura (0.48%) was similar for the value found by Uekane et al. [11] (0.5%). Table 7. Centesimal composition of in natura and osmotically dehydrated sapodilla.
The contents of proteins, lipids, carbohydrates, fibers and TCV of the dehydrated fruit was significantly higher (p < 0.05) than the in natura fruit, a fact explained by the dehydration of the fruit, consequently elevating the concentrations of these nutrients. This dehydration allowed significant loss of water from the fruit (53.23%) ( Table 7). The water activity (Aw), soluble solids and pH data are shown in Table 8. It is possible to observe that the soluble solid contents of the sapodilla dehydrated increased in relation to sapodilla in natura. This fact was explained by the concentration of sapodilla's natural sugars and the incorporation of sucrose during the process. The pH remained practically the same after the drying process. As for Aw, the dehydrated fruit is characterized as an intermediate moisture fruit. In another study, several parameters for fresh and lyophilized sapodilla pulp values were analyzed: 15.67 • Brix and 65.50 • Brix were observed, respectively. The pH values of fresh sapodilla (5.55) and lyophilized fruit (5.58) were also similar [20]. After osmotic dehydration, the sapodilla was submitted to drying in a greenhouse, in a humidity of 24.34%. This value is within the limits established by Brazilian legislation [40].

Structural Analysis of Osmotically Dehydrated Fruit
Over the last few years, there have been changes in the field of osmotic processing research and, in addition to the evaluation of macroscopic aspects, it has been sought to observe the process on a microscopic scale, because view of the anatomy and physiology of plant tissue and its cells is useful for understanding mass transfer phenomena [35]. Figure 6A,B shows the cellular structures of sapodilla subjected to the osmotic dehydration process, followed by drying. In Figure 6A, it can be observed that the cells are disordered, there is no uniformity in size due to the wrinkling caused by the loss of water during osmotic dehydration and the turgor of the cells is reduced.
Plant tissues submitted to the osmotic process tended to have cells with smaller volumes and sizes than in natura tissue [41]. Sapodilla cells in natura had a mean size of 123.7 µm × 105 µm, and osmotically dehydrated sapodilla showed cells with a mean size of 121.9 µm × 97.55 µm.
research and, in addition to the evaluation of macroscopic aspects, it has been sought to observe the process on a microscopic scale, because view of the anatomy and physiology of plant tissue and its cells is useful for understanding mass transfer phenomena [35]. Figure 6A,B shows the cellular structures of sapodilla subjected to the osmotic dehydration process, followed by drying. In Figure 6A, it can be observed that the cells are disordered, there is no uniformity in size due to the wrinkling caused by the loss of water during osmotic dehydration and the turgor of the cells is reduced. Plant tissues submitted to the osmotic process tended to have cells with smaller volumes and sizes than in natura tissue [41]. Sapodilla cells in natura had a mean size of 123.7 μm × 105 μm, and osmotically dehydrated sapodilla showed cells with a mean size of 121.9 μm × 97.55 μm. Figure 6B shows the rupture of the cell walls in a certain area, emphasizing that most of the cells remained with their structure intact. Some factors may contribute to the rupture of cell walls, such as lower cell wall resistance due to pectin solubilization, high osmotic pressure which generates changes in the distribution of pressure gradients in the tissue and causes the cells that are in direct contact with the osmotic solution to lose water and release the turgor pressure, and cell wrinkling occurs [41,42]. With the loss of water, denaturation of the protein occurs, resulting in damaged membranes. This type of damage leads to a decreased cell wall and cell membrane, and possibly cell death [39].
The microstructural differences induced by the application of osmotic dehydration in autumn olives are also shown by the images. The analysis carried out on the osmotically dehydrated fruit showed that the microstructure of the fruit became smoother, with some cracks on the surface. This observation denoted that the osmotic dehydration process  Figure 6B shows the rupture of the cell walls in a certain area, emphasizing that most of the cells remained with their structure intact. Some factors may contribute to the rupture of cell walls, such as lower cell wall resistance due to pectin solubilization, high osmotic pressure which generates changes in the distribution of pressure gradients in the tissue and causes the cells that are in direct contact with the osmotic solution to lose water and release the turgor pressure, and cell wrinkling occurs [41,42]. With the loss of water, denaturation of the protein occurs, resulting in damaged membranes. This type of damage leads to a decreased cell wall and cell membrane, and possibly cell death [39].
The microstructural differences induced by the application of osmotic dehydration in autumn olives are also shown by the images. The analysis carried out on the osmotically dehydrated fruit showed that the microstructure of the fruit became smoother, with some cracks on the surface. This observation denoted that the osmotic dehydration process affected the overall mass transport properties of the tissue. In addition, the cells shrank and some collapsed after water loss [43].
Similar structural changes were found after the dehydration of pumpkin cylinders and guava halves. In the in natura vegetables, the cells were swollen, and after dehydration, there was non-homogeneous shrinkage of these cells. In the cellular tissue of the guava, there was extensive cellular plasmolysis and the cells appeared to be deformed and wrinkled [44,45].

Conclusions
This study reports the development of an effective process for the osmotic dehydration of sapodilla fruit. The results show that moisture loss and solid gains were influenced by the temperature and concentration of the osmotic solution. In addition, the osmotic solution concentration and immersion time had significant influence on the Dehydration Efficiency Index. Based on moisture loss, two samples were subjected to sensorial analysis. Sample 9 (submitted to a sucrose solution of 50 • Brix, under operation temperature of 40 • C by 165 min) obtained the highest scores of acceptability and purchase intention. It also showed compliance with the microbiological standards and current Brazilian legislation for dehydrated fruits. Finally, the osmotic treatment generated turgor reduction and the rupture of sapodilla cell walls. Taken together, the data obtained indicate that the studied process is a good alternative for the conservation of sapodilla.  Informed Consent Statement: Informed consent was obtained from all subjects involved in the study.

Data Availability Statement:
The data presented in this study are available on request from the corresponding author.

Conflicts of Interest:
The authors declare to have no conflict of interest.