Cocoa Spread with Grape Seed Oil and Encapsulated Grape Seed Extract: Impact on Physical Properties, Sensory Characteristics and Polyphenol Content

The aim of this study was to utilize grape pomace, as a polyphenol-rich by-product of wine production, in the manufacture of enriched cocoa spread. The formulation of the cocoa spread has been modified by substitution of refined sunflower oil with cold-pressed grape seed oil. The spread with grape seed oil (Cg) was further enriched with grape seed extract encapsulated on maltodextrins (E), where 10% and 15% of E was added to Cg obtaining the samples Cg10 and Cg15. The results showed an increase in volume-weighted mean in spread samples, from 19.17 μm in Cg to 19.71 μm in Cg10 and 21.04 μm in Cg15. Casson yield stress and Casson viscosity significantly (p ˂ 0.05) increased from 16.41 Pa and 1.58 Pa·s in Cg to 29.45 Pa and 5.70 Pa·s in Cg15 due to the reduction of the fat-phase content in enriched spreads. The addition of E had no significant effect on the melting temperature (Tpeak) of the enriched spreads, while increasing the amount of E significantly (p ˂ 0.05) increased their hardness. The incorporation of grape seed oil in the cocoa spread formulation contributed to an increase in total polyphenols and flavonoids. Moreover, the addition of 10% and 15% of E to Cg resulted in approximately 1.5× and 2× higher content of phenolic compounds in Cg10 and Cg15 compared to control spread with sunflower oil (Cs). Flavonoids increased from 0.43 mg CE/g in Cs to 0.74 mg CE/g in Cg 10 and 1.24 mg CE/g in Cg15. Encapsulates positively affected sensory characteristics of enriched spread samples by reducing their grape seed oil aroma and sweetness.


Introduction
Consumers are increasingly seeking food that will prevent chronic illness and optimize their health. Global market size of functional food products is estimated at USD 74 billion [1] and it is estimated to grow by approximately 10% per year [2]. The fact is that the food industry is producing large amounts of waste of which management and disposal is a serious environmental problem. Nowadays, a new process for the controlled disposal of waste is converting these materials into other bio-products. One of the main by-products generated by the wine industry is grape pomace, which is composed of grape seeds, skin and stem remains [3]. Over 0.2 kg of grape pomace is produced per 1 kg of grapes, which contains approximately 25% seeds. Bearing in mind that world grape production in 2020 was 78,034,332 t [4], more than 0.3 kg of solid side-stream waste is generated per kg of freshly mashed grape fruit [5]. Several studies have shown that grape seed (dry base) contains about 35% crude fiber, 29% extractable components, 7-20% oil, 11% protein, 3% minerals and 7% moisture [3].

Plan of Experiments
In the first stage of the experiment the control sample of the cocoa spread was created with the addition of refined sunflower oil (sample Cs) while in the second stage the sunflower oil was replaced with cold-pressed grape seed oil to create cocoa spread with grape seed oil (sample Cg).
Cocoa spread was produced in a laboratory ball mil ("Masino Produkt", Crvenka, Serbia) with a capacity of 5 kg. The temperature in the ball mill was 40 • C, with a speed of 50 rpm. After 90 min of milling, the spread was dosed into sterile plastic cups.
In the third stage of the experiment, cocoa spread mass with grape seed oil was substituted with the addition of 10% (sample Cg10) and 15% (sample Cg15) of encapsulated grape seed extract (E) obtained from the cake remaining after oil extraction. After the cocoa spread with grape seed oil was produced in the laboratory ball mill under the same conditions, the spread mass was dosed in a plastic bucket; the mass of the spread was then measured. The specific mass of E was added and the spread mass was poured into a laboratory homogenizer with a capacity of 5 kg ("Masino Produkt", Crvenka, Serbia). The spread was homogenized with encapsulates for 30 min at 40 • C and dosed into sterile plastic cups.

Preparation of Grape Seed Encapsulate
Grape seeds were milled in a domestic blender (Bosch, MMB21P0R/01, Germany) and mean particle size of the sample was determined by sieving through the vibro-sieve set (CISA Cedaceria Industrial, Spain). The mean particle size for the red and white grape seeds was 0.578 mm. Supercritical fluid extraction (SFE) was utilized in order to remove oil from the sample according to the previously optimized procedure [9]. Defatted grape seed samples were used as the raw material for the recovery of polyphenols and production of powder extract, i.e., grape seed encapsulate.
Ultrasound-assisted extraction (UAE) with previously optimized conditions was used for production of liquid extract. UAE was conducted in a bath sonicator (EUP540A, Euinstruments, France) at a frequency of 40 KHz, temperature of 56 • C, ethanol concentration of 53% and extraction time of 29 min [24]. Extraction of 30 g of a raw material with 300 mL of extraction solvent (1:10, w/v) in 500 mL glass flasks was done in order to prepare a sufficient volume of liquid extract for the spray drying (4 L).
Liquid extracts obtained by UAE were dried using a spray-drying process in the pilot Anhydro spray-dryer plant (APV Anhydro AS, Denmark) according to the procedure previously described by Lim et al. [25]. The feed was preheated to 50 • C on a heating plate with magnetic stirrer. Processing temperatures for spray drying were 150 • C for the inlet temperature and 70-75 • C for the outlet temperature. Liquid feed was transferred to the drying chamber using a laboratory peristaltic pump (FH100 Series, Thermo Scientific, Waltham, MA, USA) with 1.36 L/h flow rate. Maltodextrin was added as carrier agent with a 50:50 (m/m) ratio to grape seed solid concentration to the liquid extract.

Particle Size Distribution of Encapsulated Grape Seed Extract and Cocoa Spread Samples
The particle size distribution of the encapsulates and cocoa spread samples was determined by the Mastersizer 2000 laser diffraction particle size analyzer (Malvern Instruments, Malvern, UK). The encapsulates were dispersed in air using a Scirocco dispersion unit, while spreads were dispersed in sunflower oil using the Hydro 2000 µP dispersion unit. The dispersed samples were added to the sunflower oil at ambient temperature until an adequate obscuration was obtained (10-20%). The results were quantified as a volume-based particle size distribution by means of the Mastersizer 2000 software (version number 5.60, Malvern, UK).

Rheological Properties of Cocoa Spread Samples
The rheological properties of the samples were determined by a rotational rheometer, the Rheo Stress 600 (Haake, Karlsruhe, Germany), at a temperature of 40 ± 1 • C [26]. The flow curves were determined by applying the method of the hysteresis loop using a concentric cylinder system (sensor Z20 DIN). The shear rate was first increased from 0 s −1 to 60 s −1 over a period of 180 s, then kept constant for 60 s at the max. speed of 60 s −1 before being reduced from 60 s −1 to 0 s −1 within 180 s.

Thermal Properties of Cocoa Spreads
The melting properties of cocoa spread samples were measured using a Differential Scanning Calorimeter DSC Q100 (TA Instruments). Approximately 5 mg of samples were weighed into aluminum pans. The hermetically sealed pans were then heated from 10 to 50 • C (5 • C/min) in the DSC using an empty aluminum pan as reference. The onset temperature (T onset ), peak maximum (T peak ), conclusion temperature (T end ) and enthalpy of melting (H melt ) were automatically calculated after integrating the melting peaks using data analysis software (TA Instruments, New Castle, DE, USA) [27].
The cocoa spread samples were heated from 10 • C to 50 • C with a heating rate of 5 • C per minute using a Differential Scanning Calorimeter DSC 910, Thermal Analyzer 990 and Dynamic Mechanical Analyzer (TA Instruments, New Castle, DE, USA). 5 mg of the spread sample was measured into aluminum pans and the pierced covers were sealed in place. An empty, hermetically sealed aluminum pan was used as a reference.
The melting properties of the spread samples were defined using DSC parameters: onset temperature (T onset ), peak temperature (T peak ) and conclusion temperature (T end ). T onset is the temperature at which a specific crystal form starts to melt, T peak is the temperature at which melting rate is the greatest and T end is the temperature at which melting ends [28].

Textural Properties of Cocoa Spreads
Textural characteristics of cocoa spread samples were analyzed using a Texture Analyzer TA.XT Plus (Stable Micro System, Godalming, UK). The maximum force (kg) and work of shear (kg·s) were measured during the analysis by penetration at an ambient temperature of 21 ± 1 • C according to the Chocolate Spread-SPRD2_SR_PRJ method. Each sample was placed in the cone sample holder and excess sample was scraped off with a knife. Then, the filled cone sample holder was placed in the base holder and the 45º cone probe with a diameter of 38 mm penetrated the samples at 3 mm/s. The obtained maximum force indicates the hardness of the cocoa spread, and the size below the surface of the force-dependence curve from the time represents the work of shear in the applied force.
The maximum force (kg) and work of shear (kg·s) were measured during the analysis. The maximum force is defined as the hardness at the specific penetration depth, while the size below the surface of the obtained force-dependence curve (F) from the time (t) represents the work of shear in the applied force.

Sensory Analyses
The sensory panel was comprised of 9 assessors (5 women and 4 men), all food technologists. They were selected according to the guidelines of the ISO 8586:2012 standards [29]. Ethical review and approval were waived for this study since the participation was voluntary. All data were anonymous. Informed consent was obtained from all subjects involved in the study. The attribute list was obtained through discussion with the panel leader. Five cocoa spread samples representing a wide range of sensory characteristics were presented to the assessors. Through a consensus process they agreed on the most adequate attributes to fully describe the dynamics of the sensory characteristics of the samples. Next, sample evaluation cards with the panelist's list of definitions of attributes and their lowest and highest intensity were prepared.
The final list of attributes included 10 attributes covering appearance, aroma, flavor and texture to be used in the examination. The panelists were asked to rate the intensity, using a 7-point scale, of each attribute [30]: color uniformity (1: non-uniform to 7: uniform); glow (1: matte to 7: oil migration); hardness, i.e., the ease with which samples could be spread on a biscuit (1: extremely soft to 7: extremely hard); graininess, i.e., the proportion of small solids between teeth during chewing (1: smooth to 7: grainy); melting, i.e., the time it takes for spread to transform into a liquid in the mouth (1: quick to 7: slow); cocoa flavor, or characteristic taste related to the presence of cocoa (1: poorly expressed to 7: very expressed); grape seed oil flavor (1: non-existent to 7: very expressed); sweetness, or characteristic taste related to the presence of sucrose (1: poorly expressed to 7: very expressed); and grape seed oil taste (1: non-existent to 7: very expressed).
The tests were conducted in the sensory laboratory at the Faculty of Technology Novi Sad, in individual cabinets illuminated with white light, designed in accordance with ISO 8589 [31]. The cocoa spread samples were served at room temperature (21 • C) on three-digit-numbered plastic cups. Mineral water at room temperature and diced peeled apple were served between sample servings. The average point number was calculated for each sample.

Polyphenol Content and Antioxidant Activity in Cocoa Spread Samples
Prior to spectrophotometric analysis, cocoa spread samples were defatted according to a modified method published by Belščak et al. [32]. First, 8 g of spread was mixed with 20 mL of hexane in an Erlenmeyer flask and placed in an orbital shaker for 10 min (150 rpm). The sample was then centrifuged at 3000 rpm for 10 min. Supernatant was separated from sediment. The sample-defatting step was repeated 3 times. Once obtained, the defatted solid part was air-dried for 24 h. Phenols were extracted from the defatted sample with 10 mL of 70% methanol using an ultrasound bath (EUP540A, Euinstruments, France) for 30 min at room temperature. The sample was then centrifuged at 3000 rpm for 10 min and the supernatant was utilized for further analysis. The methanolic extracts obtained were tested in terms of total phenolic content (TP), total flavonoid content (TF), ABTS + and DPPH radical-scavenging activity and ferric reducing antioxidant power according to methods published by Pavlić et al. [10]. All spectrophotometric analyses were performed in duplicate.

Statistical Analysis
All experiments were performed in triplicate except for the sensory analysis including 9 panelists and the spectrophotometric assays, which were performed for 6 repetitions. The obtained results were statistically tested using the ANOVA method and the means were compared by one-factor analysis at variance with subsequent comparisons by Duncan's test at a significance level at 0.05 using Statistica 13.3 software (TIBCO Software Inc., Paolo Alto, CA, USA, 2016).

The Impact of Grape Seed Oil and Encapsulated Grape Seed Extract on Particle Size Distribution and Rheological Properties of Cocoa Spreads
Since cocoa spread presents a complex multiphase system of different solid particles (sugar, cocoa powder, milk powder, etc.) dispersed in a continuous fat phase, its particle size distribution, viscosity and consistency largely depend on the milling process during production as well as on the type of fat used [21]. Consumer acceptance strongly depends primarily on appearance and taste, but also very much on mouth feel, which itself mainly depends on the particle size and the viscosity of the molten chocolate or cocoa spread mass [33]. Figure 1 shows the impact of grape seed oil and E on the particle size distribution of cocoa spread samples.
All experiments were performed in triplicate except for the sensory analysis including 9 panelists and the spectrophotometric assays, which were performed for 6 repetitions. The obtained results were statistically tested using the ANOVA method and the means were compared by one-factor analysis at variance with subsequent comparisons by Duncan's test at a significance level at 0.05 using Statistica 13.3 software (TIBCO Software Inc., Paolo Alto, CA, USA, 2016).

The Impact of Grape Seed Oil and Encapsulated Grape Seed Extract on Particle Size Distribution and Rheological Properties of Cocoa Spreads
Since cocoa spread presents a complex multiphase system of different solid particles (sugar, cocoa powder, milk powder, etc.) dispersed in a continuous fat phase, its particle size distribution, viscosity and consistency largely depend on the milling process during production as well as on the type of fat used [21]. Consumer acceptance strongly depends primarily on appearance and taste, but also very much on mouth feel, which itself mainly depends on the particle size and the viscosity of the molten chocolate or cocoa spread mass [33]. Figure 1 shows the impact of grape seed oil and E on the particle size distribution of cocoa spread samples. Replacing sunflower oil with grape seed oil leads to a barely noticeable difference in the particle size distribution curve. Encapsulated bioactive components (E) also have a very desirable particle size distribution as an additive in the production of cocoa spread products, but also contain particles in the range of 100-500 μm which leads to an increase in the volume mean diameter D [4,3] of samples Cg10 and Cg15 (Table 1). Replacing sunflower oil with grape seed oil leads to a barely noticeable difference in the particle size distribution curve. Encapsulated bioactive components (E) also have a very desirable particle size distribution as an additive in the production of cocoa spread products, but also contain particles in the range of 100-500 µm which leads to an increase in the volume mean diameter D [3,4] of samples Cg10 and Cg15 (Table 1). Cocoa spread with grape seed oil has significantly (p < 0.05) higher values for d (0.1) and d (0.5) compared to cocoa spread with sunflower oil, while their values for d (0.9) are not significantly different. However, the volume mean diameter D [3,4] of Cg is significantly (p < 0.05) higher (19.60 µm) compared to Cs (19.17 µm). Addition of 10 and 15% of E to spread with grape seed oil lowers the values of d (0.1) and d (0.5) in Cg10 and Cg15 and at the same time significantly (p < 0.05) increases the values of d (0.9) in accordance with the added amount. An increase in the value of this parameter affects the increase in D [3,4] in samples Cg10 (19.71 µm) and significantly (p < 0.05) in Cg15 (21.04 µm). Despite small differences in D [3,4], all samples have mean diameters in the desired interval (15-30 µm) that provides the appropriate rheological and sensory characteristics of chocolate and cocoa-related products [33].
Cocoa spread, like chocolate liqueur, exhibits thixotropic properties characterized by yield stress and plastic flow [34]. Figure 2 shows the influence of grape seed oil and E on the flow properties of cocoa spread samples at production temperature (40 • C). and d (0.5) compared to cocoa spread with sunflower oil, while not significantly different. However, the volume mean diame cantly (p ˂ 0.05) higher (19.60 μm) compared to Cs (19.17 μm). E to spread with grape seed oil lowers the values of d (0.1) an and at the same time significantly (p ˂ 0.05) increases the valu with the added amount. An increase in the value of this param D [4,3] in samples Cg10 (19.71 μm) and significantly (p ˂ 0.05) i small differences in D [4,3], all samples have mean diameters 30 μm) that provides the appropriate rheological and sensory and cocoa-related products [33].
Cocoa spread, like chocolate liqueur, exhibits thixotropi by yield stress and plastic flow [34]. Figure 2 shows the influe on the flow properties of cocoa spread samples at production The cocoa spread samples exhibit a thixotropic type of fl Figure 2 that the substitution of sunflower with grape seed oil of cocoa spread mass at lower shear rates (shown by the ascen cosity is very similar at higher shear rates and when reducing 1/s. Also, it is very noticeable that the addition of encapsul components to cocoa spread with grape seed oil significantly accordance with the added amount. This is also indicated by shown in Table 2. The flow curves of the cocoa spreads we model to get the following parameters: Cason yield stress The cocoa spread samples exhibit a thixotropic type of flow. It can be noticed from Figure 2 that the substitution of sunflower with grape seed oil has an impact on the flow of cocoa spread mass at lower shear rates (shown by the ascending curve) while the viscosity is very similar at higher shear rates and when reducing shear rate from 60 1/s to 0 1/s. Also, it is very noticeable that the addition of encapsulated grape seed bioactive components to cocoa spread with grape seed oil significantly increases the viscosity in accordance with the added amount. This is also indicated by the rheological parameters shown in Table 2. The flow curves of the cocoa spreads were fitted using the Casson model to get the following parameters: Cason yield stress (Pa) and Casson viscosity (Pa·s).
Although the substitution of sunflower with grape seed oil in the cocoa spread formulation significantly (p < 0.05) increases the value of Casson yield stress, it doesn't have the significant impact on Casson viscosity. The higher yield stress of Cg and the difficult flow at lower shear rates also influences the significantly (p < 0.05) higher value of the thixotropic curve area of this sample compared to the control Cs. The addition of 10 and 15% of E to cocoa spread with grape seed oil significantly (p < 0.05) increases the values of Casson yield stress, Casson viscosity and thixotropic curve area in accordance with the added amount of E. The fact is that the fat phase coats the solid particles in molten cocoa spread and reduces resistance to flow; thus, reducing fat amount through the addition of E causes a higher incidence of particle interaction that makes initial flow more difficult and increases the viscosity of enriched spread samples in accordance with the added amount.

The Impact of Grape Seed Oil and Encapsulated Grape Seed Extract on Thermal and Textural Properties of Cocoa Spread
Fasina et al. [35] investigated melting characteristics of different vegetable oils. The results showed that grape seed oil has lower values of all DSC parameters compared to sunflower oil. However, the substitution of sunflower oil with grape seed oil in the cocoa spread formulation doesn't have significant influence on T onset and T peak values, while it significantly (p < 0.05) decreases the value of T end in Cg compared to Cs ( Table 3). The addition of 10% and 15% of E to Cg doesn't significantly affect the values of T onset and T peak , while 15% of E significantly (p < 0.05) increases the value of T end . The replacement of sunflower oil with grape seed oil also doesn't have a significant effect on hardness and work of shearing. However, the decrease in the amount of the fat phase in enriched cocoa spreads caused by the addition of 10 and 15% of E to Cg leads to a significant increase (p < 0.05) in hardness and work of shear values in Cg10 and Cg15 compared to the control sample Cg, whereas the values of textural parameters of Cg15 are significantly (p < 0.05) higher than values of Cg10.

The Impact of Grape Seed Oil and Encapsulated Grape Seed Extract on Sensory Characteristics of Cocoa Spread
The values of color uniformity, glow, hardness, graininess, cocoa flavor and sweetness do not differ significantly between Cs and Cg (Table 4).
However, Cg has significantly (p < 0.05) highest mean values for grape seed oil flavor and grape seed oil taste compared to all examined samples. Sagdic et al. [23] concluded that the addition of grape seed oil to probiotic ice cream increased the functional properties of enriched ice cream without causing a negative sensory effect. This was not the case with the addition of grape seed oil to cocoa spread, where the panelists concluded that the flavor and taste have been made slightly worse compared to the control sample containing sunflower oil. However, the addition of both amounts of E to Cs reduces the expression of grape seed oil flavor and grape seed oil taste, even significantly (p < 0.05) between Cg 10 and Cg15. On the other hand, both amounts of E significantly negatively affect glow, hardness and graininess in Cs10 and Cs15, with significant difference (p < 0.05) between their values. Furthermore, only 15% of E causes significant (p < 0.05) reduction of melting and lowers cocoa flavor in Cs15 compared to Cs and Cs10.

The Impact of Grape Seed Oil and Encapsulated Grape Seed Extract on Polyphenol Content of Cocoa Spread
Waste fractions of food processing are promising sources of polyphenols (i.e., red grape pomace), which are in high demand because of their bioactivities [36]. Red grape pomace consists mainly of grape seeds and skin. This winemaking by-product contains a wide range of phenolic compounds such as phenolic acids, flavonols, flavanols, anthocyanidins, stilbenes, tannins and their derivatives [37,38]. Thus, adding encapsulated red grape pomace would increase polyphenolic content of the cocoa spread ( Figure 3). The addition of encapsulated grape seed extract in amounts of 10 and 15% results in ~1.5× and ~2× higher content of phenolic compounds in Cg10 and Cg15 sample when compared to the control sample (Cs). Encapsulates significantly enhance total flavonoid content of Cg10 (0.738 mg CE/g; ~2× fold) and Cg15 (1.238 mg CE/g; ~3× fold) to an even The addition of encapsulated grape seed extract in amounts of 10 and 15% results iñ 1.5× and~2× higher content of phenolic compounds in Cg10 and Cg15 sample when compared to the control sample (Cs). Encapsulates significantly enhance total flavonoid content of Cg10 (0.738 mg CE/g;~2× fold) and Cg15 (1.238 mg CE/g;~3× fold) to an even greater extend when compared to control cocoa spread (0.435 mg CE/g). This suggests that, indeed, grape seed extract encapsulates are carriers of valuable bioactive compounds in Cg10 and Cg15 samples. Thus, consumption of polyphenol-and particularly flavonoidenriched spreads could partly satisfy the required daily intake of polyphenols. According to certain research flavonoid intake of 500 mg/day or higher could be associated with lower risk of cardiovascular events and/or mortality [39]. Thus, polyphenol-enhanced diets seems to be linked with health benefits in humans and could be used as a valid tool for the prevention of various chronic diseases [39]. Addition of plant encapsulates or powders in confectionary products is not an entirely novel approach. As it was reported in a recent study, blackberry juice encapsulates were used to enrich white chocolate with polyphenols [40]. Results indicated that chocolate with added blackberry juice encapsulates (80 g/kg) had significantly higher polyphenolic content (145.86 g/100 g) compared to control chocolate (40.75 g/100 g), while pleasant fruity flavor and reduced sweetness improved the sensory attributes of chocolate with encapsulates. Furthermore, usage of grape seed powder extract resulted in a higher polyphenol bioavailability when grape-powderenriched white chocolate (in saliva = 31.8 mg/kg; in gastric juice = 130.9 mg/kg; in intestinal juice = 154.2 mg/kg) was in vitro digested compared to control (in saliva = 0.38 mg/kg; in gastric juice = 195.1 mg/kg; in intestinal juice = 154.2 mg/kg) [41]. However, to the best of our knowledge, usage of winemaking by-products as polyphenol carriers in cocoa spread has not been studied before.

The Impact of Grape Seed Oil and Encapsulated Grape Seed Extract on Antioxidant Activity of Cocoa Spread
Since encapsulated grape seed extract is a carrier of phenolic compounds which are well-known antioxidant agents, it is rather expected that antioxidant activity of Cg10 and Cg15 would be significantly higher compared to control (Cs) (Figure 4). Such an observation was concluded for all in vitro antioxidant assays performed.
Substitution of refined sunflower oil with grape seed oil also increases antioxidant power in cocoa spread. It could be speculated that the higher quantity of polyphenols and/or other antioxidants naturally present in cold-pressed oils [42] was retained in the defatted residue of Cg compared to Cs. Regardless, products rich in fatty content such as cocoa spread are prone to lipid oxidation. This process is undesirable in confectionary products since it negatively alters the organoleptic properties via the appearance of rancid flavor. One viable strategy to prevent lipid oxidation and to extend the shelf-life of cocoa spreads is addition of polyphenol-rich plant extracts. Indeed, addition of up to 3% of raspberry and blueberry extract significantly increased antioxidant potential and lipid oxidative stability of chocolate [43] supporting viability of such strategy.

Conclusions
From a sensory point of view, the replacement of refined sunflower oil with coldpressed grape seed oil in the formulation of cocoa spread has no significant effect on the color and glow of the spread, nor on the texture and melting properties, but, at the same time, contributes to a very pronounced grape seed oil aroma which is not common in this type of confectionery product. However, the addition of encapsulated grape seed extract has a positive effect on the flavor and taste of the enriched spread samples by reducing the grape seed oil aroma and sweetness. Grape seed oil also increases the total polyphenols and flavonoids of the cocoa spread, as the addition of 10% and 15% of encapsulated grape seed extract to cocoa spread with grape seed oil further increases total polyphenols (1.5-fold in Cg10 and 2-fold in Cg15 compared to the control spread with sunflower oil) and total flavonoids. Future research will aim to increase the share of grape seed oil in the cocoa spread formulation that includes encapsulated seed extract and grape seed oil in order to increase the fat-phase content in the enriched cocoa spread samples, thereby reducing their viscosity and hardness.