Comparison of Different Drying Methods on the Volatile Components of Ginger (Zingiber officinale Roscoe) by HS-GC-MS Coupled with Fast GC E-Nose

Ginger (Zingiber officinale Roscoe) is one of the most popular spices in the world, with its unique odor. Due to its health benefits, ginger is also widely used as a dietary supplement and herbal medicine. In this study, the main flavor components of gingers processed by different drying methods including hot air drying, vacuum drying, sun-drying, and vacuum-freeze drying, were identified on the basis of headspace-gas chromatography coupled with mass spectrometry (HS-GC-MS) and fast gas chromatography electronic-nose (fast GC e-nose) techniques. The results showed that the ginger dried by hot air drying exhibited high contents of volatile compounds and retained the richest odor in comparison with those dried by other methods, which indicated that hot air drying is more suitable for the production of dried ginger. Sensory description by fast GC e-nose exhibited that ginger flavor was mainly concentrated in the spicy, sweet, minty, fruity, and herbaceous odor. The relative content of the zingiberene was significantly higher in the hot air drying sample than those by other methods, suggesting that dried ginger by hot air drying can retain more unique spicy and pungent odorants. Furthermore, the results of chemometrics analyses showed that the main variance components among the samples by different drying methods were α-naginatene, (+)-cyclosativene, and sulcatone in HS-GC-MS analysis, and α-terpinen-7-al, dimethyl sulfide, and citronellal in fast GC e-nose analysis. For comparison of fresh and dried gingers, terpinolene, terpinen-4-ol, 2,4-decadienal, (E, Z)-, and linalool were considered the main variance components. This study generated a better understanding of the flavor characteristics of gingers by different drying methods and could provide a guide for drying and processing of ginger.


Introduction
Ginger (Zingiber officinale Roscoe) is one of the hot spices belonging to the Zingiberaceae family [1]. Due to its high nutritional and medicinal values, ginger is also extensively used as a dietary supplement, herbal medicine, and flavoring agent [2]. In addition to being eaten fresh, ginger is often processed into dried products and added to many desserts and beverages, such as ginger tea, ginger candies, and ginger beer [3,4]. Modern studies found that gingerols, shogaols, terpenes, and sugars are the key bioactive constituents of ginger, and moreover, these compounds are considered as the essential part of the pungency and aromatic flavor in ginger [5][6][7]. Some biological activities of ginger, including antioxidant [8], anti-inflammatory [9], antimicrobial activity [10], and immune-modulatory activity [11], have been reported, which have indicated the benefits of ginger and its products for humans.
The FG slices were placed on a clean tray and directly dried in an electric thermostatic drying oven (DHA-9070A, Shanghai Jinghong Experimental Equipment Co., Ltd., Shanghai, China) at constant temperatures of 50, 60 and 70 • C. All samples were dried to a moisture content of approximately 10%.

Vacuum Drying Process (VD)
The vacuum drying process was carried out in a vacuum drying oven (DZF-6090, Shanghai Augem Instrument Manufacturing Co., Ltd., Shanghai, China). The FG slices were placed on a clean tray and dried with vacuum pressure set of 0.1 MPa at 50, 60 and 70 • C. All samples were dried to a moisture content of approximately 10%.

Sun Drying (SD)
The FG samples were evenly laid flat on the trays and left to dry in a sunny and well-ventilated location. The average temperature was 25 • C, the drying time lasted for 3 days, and the moisture content was controlled at approximately 14%.

Vacuum-Freeze Drying (VFD)
The FG slices were firstly pre-frozen in an ultra-low temperature refrigerator at −80 • C for 12 h, then quickly placed into a freeze-dryer (Labconco, FreeZone 6 freeze-dryer, Kansas City, MO, USA). The cold trap temperature and vacuum pressure were set to −80.0 • C and 1.0 Pa, respectively. The whole drying time lasted 48 h, and the moisture content was controlled at approximately 10%.

Preparation of Dried Ginger Powder
DG samples prepared by different drying methods were crushed into powder by a high-speed grinder (FW-80, Tianjin Taisite Instrument Co., Tianjin, China), and then these powders were all passed through 50 mesh sieves for experimental use.

Color Measurements
The color characteristics of the FG and DG samples obtained by different drying methods (HAD, VD, SD, and VFD) were measured by a chroma analyzer (CM-5, KONICA MINOLTA, Tokyo, Japan). The spectrophotometer was first calibrated by a white plate, and the powder was evenly flat in the measuring dish, keeping the same weight and thickness of all samples. Six determinations were carried out for each drying method of ginger samples. According to the CIELAB color space theory of the International Commission on Illumination, the parameters of L* value (darkness to brightness), a* value (greenness to redness), and b* value (blueness to yellowness) were collected [27]. The total color difference (∆E) was used to describe the color change of DG with the following equation [12]: The values of L 0 *, a 0 *, and b 0 * were measured by fresh ginger. The values of chroma and hue angle (H • ) were used to describe the abundance of color and were calculated as follows: H • = tan −1 (b*/a*) when a* ≥ 0 and b* ≥ 0 H • = 180 + tan −1 (b*/a*) when a* < 0 (4)

Analysis of Volatile Components by HS-GC-MS
The HS-GC-MS analysis was carried out in an Agilent 7890B gas chromatograph (Agilent Technologies, Santa Clara, CA, USA) coupled to an Agilent 7000C triple quadrupole mass spectrometer (Agilent Technologies, Santa Clara, CA, USA), which was equipped with an electron impact (EI) ionization chamber and worked in full scan mode. A HP-5MS quartz capillary column (30 m × 0.25 mm, 0.25 µm) was used for separation. Headspace extraction was performed by an Agilent 7697A autosampler (Agilent Technologies, Santa Clara, CA, USA).
HS conditions were as follows: 0.3 g of ginger powder was accurately weighed and added into 20 mL headspace vials. Then, the vials were capped by a special PTFE-silicon spacer and incubated at 70 • C for 10 min, awaiting injection. In addition, the quantitative tube temperature and the transfer tube temperature were set to 85 and 100 • C, respectively. The injection time was maintained at 0.5 min.
For GC conditions, the temperature of the injection port was set to 250 • C, the split ratio was set to 50:1, and the carrier gas was helium (99.999% pure) with a flow rate of 1.0 mL/min. The oven temperature program started from 50 • C, was held for 1 min, and increased to 82 • C at a rate of 8 • C/min, then increased to 106 • C at a rate of 3 • C/min, was subsequently heated to 145 • C at a rate of 4 • C/min, and was finally ramped at a rate of 15 • C/min to 230 • C, remaining there for 5 min. The total run time of the GC program was 33 min. For MS conditions, the electron ionization (EI) energy was 70 eV, and the ion source temperature was 230 • C. The transfer line temperature was set at 280 • C. A full scan mode was operated at a mass range of 50.0-500.0 amu with a quadrupole temperature of 150 • C.
Volatile components were processed in Agilent MassHunter Qualitative Analysis (B.07.00) software. R match scores and retention index (RI) values in the NIST Mass Spectral Library (NIST 14.0, National Institute of Standards and Technology, Gaithersburg, MD, USA) were used to identify unknown compounds. The RI value of each component was calculated using an n-alkane solution (C 6 to C 16 ) under the same conditions. By comparing the measured and published literature RI values and combining them with the highest R-match scores, the compound information was finally determined. Generally, the standard for determination of volatile compounds is that the difference between the experimental RIs and the theoretical values does not exceed 30 [28]. For quantitative analysis of the different components, 15 µL of n-decyl hydride (0.4 mg/mL) was added to each sample as an internal standard, and the relative content of each component was determined by the ratio of the peak area measured to that of the internal standard.
2.6. Analysis of Flavor Compounds by Fast GC E-Nose 2.6.1. Sample Incubation A headspace autosampler (PAL-RSI, Alpha M.O.S., Toulouse, France) was used to extract the flavor compounds of ginger samples. Briefly, 0.1 g DG powders were added to 20 mL specialized vials for headspace extraction. To allow the odor to saturate the headspace bottles, the incubation temperature was set to 55 • C for 10 min, with a stirring speed of 500 rpm.

Acquisition of Flavor Components
The gas incubated by headspace was injected directly into the fast GC e-nose system for odor analysis. The odor information was collected by a Heracles NEO e-nose system (Alpha M.O.S., Toulouse, France), which was combined with fast gas chromatography and the sensory functions of electronic nose. Two different polarity columns (MXT-5: a nonpolar column, 10 m × 0.18 mm × 0.4 µm; MXT-1701: a low-polar column, 10 m × 0.18 mm × 0.4 µm) were simultaneously used to monitor the concentrated odor and realize rapid separation. The flavor compounds were detected by two flame ionization detectors (FIDs).
The injection volume was set to 1000 µL at a speed of 125 µL/s, and the inlet temperature and pressure were set as 200 • C and 10 kPa, respectively. The following oven temperature program was used: initial temperature of 50 • C (2 s), increased to 100 • C with 6 • C/s, then heated to 200 • C with 2 • C/s, and finally ramped at 1 • C/s to 250 • C (10 s). Hydrogen was used as the carrier gas at a constant flow of 1.0 mL/min. Each sample was repeatedly measured three times, following the above conditions.

Qualitative Analysis of Flavor Components
N-Alkanes C 6 to C 16 (Restek France, Lisses, France) were detected as the same method as ginger samples and then RI values of each unknown component were calculated according to MXT-5 and MXT-1701 columns. The flavor compounds were identified by comparing the measured RI values with Kovats relative retention index in the Arochem Base database.

Statistical Procedures
Principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), and orthogonal partial least squares discriminant analysis (OPLS-DA) were performed using SIMCA-P software (Version 14.1, Umetrics, Sweden). IBM SPSS Statistics 26.0 software (SPSS Inc., Chicago, IL, USA) was used for significant difference analysis by Waller-Duncan's multiple-range text (p < 0.05 as the significance threshold). Histogram and Venn diagram were plotted in Origin 2021 pro (Microcal Software, Inc., Northampton, NC, USA).

Analysis of the Appearance Characteristics and Color Changes of Dried Gingers
The appearance characteristics of FG and DG by different drying methods (HAD, VD, SD, and VFD) are shown in Figure 1. In terms of shape, DG by VFD kept almost the same shape as fresh ginger, while those by HAD, VD, and SD were all shrunken. The color of DG also changed obviously by different drying conditions through visual observation. Compared to FG, the DG treated by VFD was significantly lighter in color, and the sample obtained by SD was close to white, while the samples obtained by HAD and VD were similar in color (light yellowish). Furthermore, the color of DG was quantified and characterized on the basis of CIELAB chromaticity space theory [25]. The chroma data including L*, a*, b*, chroma, hue angle, and ∆E values are listed in Table 1. The L* values of the dried gingers obtained with different methods were increased to different degrees except for HAD-70, and VFD gave the highest L* value, which indicated the brightest color. Ginger by SD showed the lowest a* value compared to FG, indicating that its color was far from the original yellow of FG. The values of chroma and hue angle suggested that ginger by VFD was colorful abundance. The ∆E is an important indicator used to measure the color changes of DG before and after processing.
The ∆E values of samples by VFD and SD samples were significantly higher than those of other processing methods, while ginger by HAD was closed to FG, indicating that vacuum freeze-drying and sun-drying had the greatest effect on color changes of DG, which is consistent with the conclusion reached by visual inspection.    ------1 Different letters ( a-g ) in the same line indicate statistically significant differences (p < 0.05, Waller-Duncan's Text). HAD-50: hot air drying at 50 °C; HAD-60: hot air drying at 60 °C; HAD-70: hot air drying at 70 °C; VD-50: vacuum drying at 50 °C; VD-60: vacuum drying at 60 °C; VD-70: vacuum drying at 70 °C; VFD: vacuum freeze drying; SD: sun drying; FG: fresh ginger. "------" indicates that the ∆E value has not been detected in FG.

Identification and Analysis of Volatile Components
In the present research, a total of 52 volatile components were identified from fresh and dried gingers. The total ion chromatography (TIC) plots and detailed information of various compounds are shown in Figure 2 and Table 2. Among these compounds, terpenes (37) were confirmed to be the main constituents of ginger flavor, accounting for more than 65% in all the volatiles, followed by ketones (4), aldehydes (4), alcohols (3), esters (2), and alkenes (2). As shown in the Venn plot ( Figure 3A), a total of 36 common

Identification and Analysis of Volatile Components
In the present research, a total of 52 volatile components were identified from fresh and dried gingers. The total ion chromatography (TIC) plots and detailed information of various compounds are shown in Figure 2 and Table 2. Among these compounds, terpenes (37) were confirmed to be the main constituents of ginger flavor, accounting for more than 65% in all the volatiles, followed by ketones (4), aldehydes (4), alcohols (3), esters (2), and alkenes (2). As shown in the Venn plot ( Figure 3A), a total of 36 common components were detected by different drying methods. The whole distribution of volatile components showed that the main compounds of FG and DG were zingiberene, β-phellandrene, β-bisabololene, β-sesquiphellandrene, α-curcumene, eucalyptol, camphene, α-pinene, and α-phellandrene, which covered the main aroma information of ginger. The relative contents of zingiberene and β-phellandrene were relatively high, both accounting for more than 25% in each tested compound, which is consistent with the result of An et al. [29]. Different drying methods showed various effects on the volatile components of ginger. For the hot-air-dried ginger, 51 aroma compounds were extracted and identified. Hot air drying at 50, 60 and 70 • C had little effect on the types of volatile composition, but there were some differences in their contents (Table S1). We found that the contents of monoterpenes (β-phellandrene, camphene, α-pinene) were significantly higher in the samples dried at 50 • C, while the contents of sesquiterpenes (zingiberene, β-bisabololene, β-sesquiphellandrene, α-curcumene) were more abundant in the samples dried at 60 • C. This suggested that the synthesis of short-chain olefins may be promoted with the increasing temperature. However, the contents of these monoterpenes slightly increased and sesquiterpenes slightly decreased when the temperature increased to 70 • C, which could be attributed to the degradation of sesquiterpenes to monoterpenes due to excessively high temperature [29].
samples dried at 50 °C, while the contents of sesquiterpenes (zingiberene, β-bisabololene, β-sesquiphellandrene, α-curcumene) were more abundant in the samples dried at 60 °C. This suggested that the synthesis of short-chain olefins may be promoted with the increasing temperature. However, the contents of these monoterpenes slightly increased and sesquiterpenes slightly decreased when the temperature increased to 70 °C, which could be attributed to the degradation of sesquiterpenes to monoterpenes due to excessively high temperature [29].   In the samples dried by VD, SD, and VFD, there were 50, 49, 37 aroma compounds identified, respectively. As shown in Figure 3B, FG contained the highest contents of volatile compounds, followed by HAD, VD, and SD gingers, while VFD ginger presented the lowest volatile content. Compared to FG, the relative contents of volatile components were significantly decreased after heat treatment (hot air or vacuum drying), which could be attributed to the effects of the hot air and vacuum; this was also demonstrated by Ding et al. [26]. In addition, ginger dried by VFD showed significant decrease in categories and contents of volatile compositions to FG, which is similar to the results of An et al. [29]. We also found that the reduced volatile components were mainly concentrated in monoterpenes, and a total of eight monoterpenes, namely, tricyclene, α-thujene, sabinene, 3-carene, α-terpinene, (Z)-ocimene, γ-terpinene, and (-)-camphor, were not detected. Studies have shown that low-temperature conditions of freeze-drying may result in a decrease in enzymatic activity and downregulation or disruption of metabolic pathways, leading to a significant decline in volatile compounds [30]. It may be a reason for the decrease in total volatiles of dried ginger by VFD. Furthermore, volatile components of frozen samples are more likely to lose by sublimation in vacuum conditions, which may be another reason for the low contents of volatile compounds in freeze-dried gingers. In general, the conventional hot air-drying method was able to retain the maximum varieties and contents of volatile components of ginger, suggesting that hot air drying is more suitable for the production of dried ginger.  In the samples dried by VD, SD, and VFD, there were 50, 49, 37 aroma compounds identified, respectively. As shown in Figure 3B, FG contained the highest contents of volatile compounds, followed by HAD, VD, and SD gingers, while VFD ginger presented the lowest volatile content. Compared to FG, the relative contents of volatile components were significantly decreased after heat treatment (hot air or vacuum drying), which could be attributed to the effects of the hot air and vacuum; this was also demonstrated by Ding et al. [26]. In addition, ginger dried by VFD showed significant decrease in categories and contents of volatile compositions to FG, which is similar to the results of An et al. [29]. We also found that the reduced volatile components were mainly concentrated in monoterpenes, and a total of eight monoterpenes, namely, tricyclene, α-thujene, sabinene, 3carene, α-terpinene, (Z)-ocimene, γ-terpinene, and (-)-camphor, were not detected. Studies have shown that low-temperature conditions of freeze-drying may result in a decrease in enzymatic activity and downregulation or disruption of metabolic pathways, leading to a significant decline in volatile compounds [30]. It may be a reason for the decrease in total volatiles of dried ginger by VFD. Furthermore, volatile components of frozen samples are more likely to lose by sublimation in vacuum conditions, which may be another reason for the low contents of volatile compounds in freeze-dried gingers. In general, the conventional hot air-drying method was able to retain the maximum varieties and contents of volatile components of ginger, suggesting that hot air drying is more suitable for the production of dried ginger.

Discrimination of the Dried Gingers by PCA and PLS-DA Analysis
To further explore the differences among the FG and DGs dried by HAD, VD, SD, and VFD, PCA and PLS-DA models were used for analysis. PCA and PLS-DA are both pattern recognition methods, and they can differentiate the samples on the basis of complex chemical information. In this study, the total values of the first two PCs were 66.9% and 7.3%, with R 2 X = 0.808 and Q 2 (cum) = 0.688, indicating that the total variation could be better explained and predicted, respectively. As shown in the score plots ( Figure 3C), the FG could be significantly distinguished from the processed samples, indicating that the volatile chemical profiles differed greatly between the fresh and dried gingers. For different drying methods, gingers by VFD and SD could be categorized separately, while the distinction between HAD and VD samples was not obvious. To determine the key volatile components affecting the differences among these samples, the PLS-DA model was employed. Permutation tests of 200 times showed that the intercepts of R 2 Y (0.165) and Q 2 Y (−0.201) were less than 0.3 and 0.05, indicating that this model was reliable and that no overfitting phenomenon existed. The variable importance factors (VIP) are distributed in Figure 3D, and 15 volatile components (VIP > 1) with the highest discrimination potential were found. Among them, α-naginatene, (+)-cyclosativene, sulcatone, citronellol acetate, 2-heptanone, and α-citral were considered to be the most critical factors that influenced the distinction of gingers by different drying methods.

Analysis of Flavor Components
The flavor components of ginger by different drying methods were sniffed by fast GC e-nose. As a new type of rapid odor analyzer, the fast GC e-nose can imitate the human sense of smell to provide sensory information of volatile components. In this work, the flavor compounds were separated using two columns (MXT-5 and MXT-1701). According to the chromatographic behaviors ( Figure 4A), the MXT-5 column was more effective in separation of ginger flavor compositions. Therefore, the MXT-5 column was used as the primary discriminator, while the MXT-1701 column was used as an auxiliary in qualification of compounds. By comparing the calculated retention index of each flavor peak with the ArochemBase database, 27 flavor compounds were identified in the dried gingers processed with different drying methods, and the sensory characteristics of flavor components were obtained in only 100 s. The relative information of the aroma components is presented in Table S2. Of all the flavor components, there were 14 terpenoids, such as zingiberene, α-curcumene, β-phellandrene, α-pinene, β-pinene, and myrcene. The relative contents of zingiberene and β-phellandrene were highest among ginger aroma, which is consistent with the result by HS-GC-MS. In addition, seven aldehydes, one alcohol, two fatty hydrocarbons, one ester, and two ethers were also identified.  Compared to traditional e-nose, the fast GC e-nose can provide sensory descrip of each flavor compounds. Generally, spicy and pungency are considered to be the m organoleptic flavors in ginger [31]. As the main contributor in aromatic odor of gin zingiberene showed the odor of spicy and pungency through e-nose analysis. Other fla compounds including myrcene, linalool, terpinen-4-ol, β-caryophyllene, α-terpinenanethole, and propanal, were also smelt as the contributors to spicy scents. Another fla component with relatively high content was β-phellandrene, which was mainly expres Compared to traditional e-nose, the fast GC e-nose can provide sensory description of each flavor compounds. Generally, spicy and pungency are considered to be the main organoleptic flavors in ginger [31]. As the main contributor in aromatic odor of ginger, zingiberene showed the odor of spicy and pungency through e-nose analysis. Other flavor compounds including myrcene, linalool, terpinen-4-ol, β-caryophyllene, α-terpinen-7-al, anethole, and propanal, were also smelt as the contributors to spicy scents. Another flavor component with relatively high content was β-phellandrene, which was mainly expressed as a minty, fruity, pleasant, herbaceous, and terpenic odor. Sweet, minty, fruity, and citrusy were considered to be the important aromas in ginger or other vegetables and fruits [31,32], and in this study, a total of 21 components exhibited these flavors (Table S2). All the flavors detected in ginger, such as spicy, sweet, minty, citrusy, herbaceous, fruity, and lemon-like., constituted the overall aromatic properties of ginger, and its distinctive odor makes it widely used in spice and functional beverages. Although the types of flavor components in gingers by different drying methods was similar, the abundance of these odors might be different. Table S3 lists the relative contents of flavor components in the samples dried by different drying methods, and Figure 4B shows the changes in odor abundance of different flavor components. Zingiberene and β-phellandrene accounting for more than 50% of the total relative content of fragrance components in gingers were important indicators for evaluating the flavor changes of gingers by different drying methods. Compared to FG, the relative content of β-phellandrene in the DG was significantly decreased, which indicated that the minty, fruity, pleasant, herbaceous, and terpenic odor of ginger were lost in large quantities after hot air drying. For the relative content of zingiberene, ginger by HAD was significantly higher than those by other drying methods, suggesting that the traditional HAD method allowed the dried gingers to retain more unique spicy and pungent odorants.

Discrimination of the Dried Gingers by PCA and PLS-DA Analysis
The odor fingerprints of ginger by different drying methods were established using the chromatography information of the MXT-5 column and MXT-1701 column ( Figure 4A). As shown in the chromatograms, the main flavor peaks were significantly different in FG and DGs by HAD (50, 60, 70 • C), VD (50, 60, 70 • C), SD, and VFD. To further distinguish the differences in gingers by various drying methods, PCA and PLS-DA models were employed.
The parameters of R 2 X and Q 2 (cum) in the PCA model were 0.808 and 0.688, respectively, indicating that the model has a strong explanatory and predictive capacity. The separation of FG and DGs dried by HAD, VD, SD, and VFD is clearly displayed in Figure 5A, indicating significant differences in their aroma substances. However, the data points of HAD at 50, 60, and 70 • C, and VD at 50, 60, and 70 • C, partially overlapped, indicating that there was no significant difference in the samples dried at different temperature. The loading scatter plot ( Figure 5B) shows that 2,4-decadienal, (E, Z)-, β-caryophyllene, α-curcumene, and zingiberene contributed greatly to the flavor production of FG, and terpinen-4-ol, α-terpinen-7-al, propanal, and geraniol may have had a greater contribution for HAD and SD samples. PLS-DA model was further conducted for all flavor peaks and exhibited a similar distinction to PCA ( Figure 5C). The main parameters of R 2 X and Q 2 (cum) were 0.935 and 0.592, respectively, indicating that the model has a strong explanatory in classification of gingers by different drying methods. To discover the characteristic flavor components in classification, the variables of V-plot ( Figure 5D) was profiled. The results showed that the VIP values of 10 flavor components were greater than 1.0, including citronellal, α-terpineol, terpinolene, 2-methylthiophene, terpinen-4-ol, anethole, hexanal, α-terpinen-7-al, propanal, and dimethyl sulfide, suggesting that they may be the main aromatic factors in the classification of different drying methods. These VIP components exhibited a spicy, sweet, minty, herbaceous, licorice, moldy, and citrus-like odor and were determined as the most potent odorants in dried gingers.

Flavor Comparison between FG and DG by OPLS-DA
PCA and PLS-DA score plots showed that the FG samples were considerably distant from the DG samples, which indicated a completely diverse aroma profile. Therefore, an OPLS-DA model was used to further explain the flavor differentials between fresh and dried gingers, and the scatter plots and flavor variables (VIP > 1.0) are shown in Figure 6. Comparing FG to HAD samples, α-phellandrene, α-pinene, β-pinene, and terpinolene had a greater effect in discrimination, which exhibited the main flavors of sweet, minty, and fruity. The main flavor differentials between FG and VD gingers were dimethyl sulfide, (E, E)-2,4-octadienal decyl acetate, 2,4-decadienal, (E, Z)-, and terpinen-4-ol. Although HAD and VD both had a heating program, the differences in flavor compositions to FG were different. As an advanced drying method, vacuum freeze drying can maintain the nutrients and shape of food products. Ginger by VFD had a significant difference to FG in flavor compounds, such as terpinolene, α-pinene, β-pinene, and 2-methylthiophene. We also found that only three components were classified as spicy odor among 14 flavor compounds (VIP > 1.0), and this may be attributed to the loss of spicy aroma through VFD. In addition, the traditional sun-dried ginger differed from FG mainly in terms of terpinen-4-ol, terpinolene, citronellal, 2,4-decadienal, and (E, Z)-, and sun-dried ginger exhibited more sweet and fruity scents. Among these variance factors, terpinolene, terpinen-4-ol, 2,4-decadienal, (E, Z)-, and linalool were considered to be the common aroma components between fresh and dried gingers.

Flavor Comparison between FG and DG by OPLS-DA
PCA and PLS-DA score plots showed that the FG samples were considerably distant from the DG samples, which indicated a completely diverse aroma profile. Therefore, an OPLS-DA model was used to further explain the flavor differentials between fresh and dried gingers, and the scatter plots and flavor variables (VIP > 1.0) are shown in Figure 6. Comparing FG to HAD samples, α-phellandrene, α-pinene, β-pinene, and terpinolene had a greater effect in discrimination, which exhibited the main flavors of sweet, minty, and fruity. The main flavor differentials between FG and VD gingers were dimethyl sulfide, (E, E)-2,4-octadienal decyl acetate, 2,4-decadienal, (E, Z)-, and terpinen-4-ol. Although HAD and VD both had a heating program, the differences in flavor compositions to FG were different. As an advanced drying method, vacuum freeze drying can maintain the nutrients and shape of food products. Ginger by VFD had a significant difference to FG in flavor compounds, such as terpinolene, α-pinene, β-pinene, and 2-methylthiophene. We also found that only three components were classified as spicy odor among 14 flavor compounds (VIP > 1.0), and this may be attributed to the loss of spicy aroma through VFD. In addition, the traditional sun-dried ginger differed from FG mainly in terms of terpinen-4-ol, terpinolene, citronellal, 2,4-decadienal, and (E, Z)-, and sun-dried ginger exhibited more sweet and fruity scents. Among these variance factors, terpinolene, terpinen-4-ol, 2,4-decadienal, (E, Z)-, and linalool were considered to be the common aroma components between fresh and dried gingers.

Comparative Analysis of HS-GC-MS and Fast GC E-Nose
For comparing the two volatile analysis techniques, dried gingers by four different drying methods (HAD, VD, SD, and VFD) can be significantly distinguished from FG ( Figures 3C and 5A), indicating that the flavor composition of FG was greatly altered after the drying process. In terms of four drying methods, gingers by HAD, VD, SD, and VFD were significantly different in aroma profile on the basis of fast GC e-nose, while in HS-GC-MS analysis, ginger by HAD showed similar flavor characteristics to VD samples. The inconsistencies may be attributed to the difference between chromatographic columns and instrument detection efficiency for volatile components. In this study, 52 volatile components were detected on the HP-5MS column in HS-GC-MS, and only 27 were detected on the MXT-column in fast GC e-nose, which demonstrated the various retention of flavor components by different columns; similar results were obtained in the research of Chinese jujube aroma [21]. However, it was notable that 12 major components, namely, α-pinene, β-pinene, myrcene, α-phellandrene, β-phellandrene, terpinolene, linalool, citronellal, terpinen-4-ol, α-terpineol, α-curcumene, and zingiberene were simultaneously identified in ginger by HS-GC-MS and fast GC e-nose, and their chromatographic behaviors were similar. Overall, both HS-GC-MS and fast GC e-nose could achieve the differentiation of ginger by different drying methods using the holistic volatile components. Although more compounds were identified in HS-GC-MS, it could not provide sensory evaluation of these flavor components, while the fast GC e-nose could recognize the main ginger aroma and provide sensory description in a very short time, which made up for the deficiency of GC-MS in flavor evaluation. Therefore, the combination of two techniques can provide a more comprehensive characterization to overall flavor variability of dried gingers by different drying methods.

Conclusions
In the present study, HS-GC-MS and fast GC e-nose techniques were firstly applied in discriminating gingers by different drying methods based on overall volatile components. A total of 52 volatile components and 27 flavor compounds were identified by HS-GC-MS and fast GC e-nose, respectively. Terpenes were confirmed to be the main volatile constituents of gingers by different drying methods, which accounted for more than 65% and represented the main odor information of ginger. The contents of ginger volatile compounds showed that FG had higher volatile contents than DG, and ginger by HAD retained the richest odor than other drying methods. Due to the effective retention of volatile components, hot air drying is more suitable for the production of DG. Sensory evaluation by fast GC e-nose showed that the ginger flavor was mainly concentrated in the spicy, sweet, minty, fruity, and herbaceous odor. The results of fast GC e-nose analysis also suggested that dried gingers by HAD can retain more unique spicy and pungent odorants, which complemented the HS-GC-MS analysis results. Chemometrics analysis further revealed that fresh ginger and dried gingers by HAD, VD, SD, and VFD were distinguished on the basis of volatile components, indicating that flavor profiles between DG and FG were significantly different. For comparison of fresh and dried gingers, terpinolene, terpinen-4-ol, 2,4-decadienal, (E, Z)-, and linalool were considered as the main variance components. The integration of the two volatile techniques not only provided a comprehensive aroma profile of gingers by different drying methods, but also provided an idea in flavor characterization for drying, storing, and processing of dried ginger or its products.