LC-QTOF-MS/MS Based Molecular Networking Approach for the Isolation of α-Glucosidase Inhibitors and Virucidal Agents from Coccinia grandis (L.) Voigt

Coccinia grandis or ivy gourd is an edible plant. Its leaves and fruits are used as vegetable in many countries. Many works on antidiabetic activity of a crude extract of C. grandis, i.e., in vitro, in vivo, and clinical trials studies, have been reported. Profiles of the antidiabetic compounds were previously proposed by using LC-MS or GC-MS. However, the compounds responsible for antidiabetic activity have rarely been isolated and characterized by analysis of 1D and 2D NMR data. In the present work, UHPLC-ESI-QTOF-MS/MS analysis and GNPS molecular networking were used to guide the isolation of α-glucosidase inhibitors from an extract of C. grandis leaves. Seven flavonoid glycosides including rutin (1), kaempferol 3-O-rutinoside (2) or nicotiflorin, kaempferol 3-O-robinobioside (3), quercetin 3-O-robinobioside (4), quercetin 3-O-β-D-apiofuranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→6)]-β-D-glucopyranoside (5) or CTN-986, kaempferol 3-O-β-D-api-furanosyl-(1→2)-[α-L-rhamnopyranosyl-(1→6)]-β-D-glucopyranoside (6), and kaempferol 3-O-β-D-apiofuranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→6)]-β-D-galactopyranoside (7) were isolated from C. grandis leaves. This is the first report of glycosides containing apiose sugar in the genus Coccinia. These glycosides exhibited remarkable α-glucosidase inhibitory activity, being 4.4–10.3 times more potent than acarbose. Moreover, they also displayed virucidal activity against influenza A virus H1N1, as revealed by the ASTM E1053-20 method.


Introduction
Coccinia grandis (L.) Voigt or ivy gourd is an edible plant under the family of Cucurbitaceae. It is a perennial climber with the synonym of Coccinia indica Wight & Arn. Ivy gourd, C. grandis, is native to many regions of tropical and subtropical Asia, America, and the Pacific. In Thailand, ivy gourd is called "Tum Lueng" and used as a common vegetable in Thai cuisine. C. grandis is also used as vegetable in many countries, for example, India, Sri Lanka, Bangladesh, Indonesia, Philippines, Cambodia, Vietnam, Malaysia, and Myanmar.

Plant Materials and Extraction Method
Leaves of Coccinia grandis (L.) Voigt were collected from Pathum Thani Province, Thailand. Fresh leaves of C. grandis (0.89 kg) were chopped into small pieces using an electric blender. It was extracted with methanol at room temperature (3 × 2.5 L; each by maceration in methanol for 3 days). A methanol extract was evaporated using a rotary evaporator to give 27.86 g of a crude extract.

UHPLC-ESI-QTOF-MS/MS Analysis
A crude extract of C. grandis leaves was analyzed by UHPLC Agilent 1290 infinity II coupled to the Agilent 6545 LC-QTOF/MS system. A crude extract (1 mg) or the standard compound was dissolved in 1 mL of methanol to make stock solution at concentrations of 1 mg/mL. A total of 100 µ L of stock solution was diluted with 900 µ L of methanol to obtain the final concentration of 100 µ g/mL. The solution then was filtered through a 0.22 µ m filter and transferred into a 2 mL LC vial. The HPLC column was Waters XBridge C18 (2.1 × 100 mm, 2.5 µ m), and at the flow rate was at 0.4 mL/min with an injection volume of 1 µL. Mobile phase consisted of 0.1% of formic acid in water (A) and 0.1% of formic acid in acetonitrile (B) The gradient elution was applied using the following conditions: 0-13 min, 5-17% B; 13-20 min, 17-100% B; 20-25 min, 100% B; and 25-27 min, 100-5%. A post-run of 6 min was set to equilibrate the column between analyses.
Dual AJS (Agilent Jet Stream) ESI was used as an interface for LC-MS system, which was arranged with specific parameters: sheath gas flow rate, 12 L/min; sheath gas temperature, 250 °C; nebulizer pressure, 45 psig; gas flow rate, 11 L/min; gas temperature, 300 °C. LC-MS was operated in full-scan mode, performing positive and negative ionizations, with a scan range from 50-1050 m/z, and the scan rate was 1 spectra per second. Auto-MS 2 was performed using fixed collision energies of 10, 20, and 40 eV. The scan rate was set at

Plant Materials and Extraction Method
Leaves of Coccinia grandis (L.) Voigt were collected from Pathum Thani Province, Thailand. Fresh leaves of C. grandis (0.89 kg) were chopped into small pieces using an electric blender. It was extracted with methanol at room temperature (3 × 2.5 L; each by maceration in methanol for 3 days). A methanol extract was evaporated using a rotary evaporator to give 27.86 g of a crude extract.

UHPLC-ESI-QTOF-MS/MS Analysis
A crude extract of C. grandis leaves was analyzed by UHPLC Agilent 1290 infinity II coupled to the Agilent 6545 LC-QTOF/MS system. A crude extract (1 mg) or the standard compound was dissolved in 1 mL of methanol to make stock solution at concentrations of 1 mg/mL. A total of 100 µL of stock solution was diluted with 900 µL of methanol to obtain the final concentration of 100 µg/mL. The solution then was filtered through a 0.22 µm filter and transferred into a 2 mL LC vial. The HPLC column was Waters XBridge C 18 (2.1 × 100 mm, 2.5 µm), and at the flow rate was at 0.4 mL/min with an injection volume of 1 µL. Mobile phase consisted of 0.1% of formic acid in water (A) and 0.1% of formic acid in acetonitrile (B) The gradient elution was applied using the following conditions: 0-13 min, 5-17% B; 13-20 min, 17-100% B; 20-25 min, 100% B; and 25-27 min, 100-5%. A post-run of 6 min was set to equilibrate the column between analyses.
Dual AJS (Agilent Jet Stream) ESI was used as an interface for LC-MS system, which was arranged with specific parameters: sheath gas flow rate, 12 L/min; sheath gas temperature, 250 • C; nebulizer pressure, 45 psig; gas flow rate, 11 L/min; gas temperature, 300 • C. LC-MS was operated in full-scan mode, performing positive and negative ionizations, with a scan range from 50-1050 m/z, and the scan rate was 1 spectra per second. Auto-MS 2 was performed using fixed collision energies of 10, 20, and 40 eV. The scan rate was set at three spectra per second with MS/MS scan range from 50-1100 m/z. Isolation width MS/MS was set at ±4 m/z, and the maximum precursor was three per cycle. The reference solutions were prepared to provide internal reference masses for mass correction in positive and negative modes of operation. The calibrant masses at m/z 121.0509 (purine) and m/z 992.0098 (HP-0921) were captured as standard ion peaks in positive mode, while the ions at m/z 112.9855 (TFA anion) and m/z 1033.9881 (HP-0921 + TFA anion) were captured in the negative mode.

MS-Based Molecular Networking
Prior to the generation of molecular networking, MSconvert, a free software from Pro-teoWizard was used to convert MS/MS data into an open file format (mzXML). The mzXML files were subjected to the GNPS (Global Natural Product Social Molecular Networking) website to create molecular networks [18]. Several parameters in MSconvert were set up, i.e., 32-bit of binary encoding precision, peak picking, and MS-Levels 1-2 were selected, while zlib compression was unchecked [19]. FTP client, WinSCP, was employed to upload all mzXML MS/MS data format using the host name ccms-ftp01.ucsd.edu. (port 21). All these data were available in the GNPS website once uploaded and ready to create the molecular networks on GNPS website (https://gnps.ucsd.edu/ProteoSAFe/static/gnps-splash.jsp, accessed on 15 August 2021) [20]. The GNPS networking parameters under the "basic option" section was arranged by keeping the precursor ion and fragment ion mass tolerance at 0.5 and 0.02 Da, respectively. This setting allows the algorithm to generate a single consensus spectrum within specific range. To produce the molecular network and lower the network complexity, the matched fragment ion was kept to a minimum of 4 (MS fragments) and the minimum cosine similarity score for connecting one node to another was set to 0.7. The TopK value was set to 10 for maintaining the edge between two nodes in a network. The cosine score is ranged between 0.7-1, where a cosine score of 1 denotes high similarity. The TopK value of 10 defines as the maximum number of nodes, which is connected to a single node. To evaluate the quality of annotation of the corresponding cosine score, several parameters such as the total of fragment ions, peak intensities, and parent mass accuracy are taken into consideration [19]. The molecular networks generated from the GNPS system were imported into Cytoscape 3.8.2 to allow more approachable visual exploration of the detectable metabolite profile in a crude extract and the correlation of standard compounds with their analogs [19].

NMR Experiments
NMR spectra were recorded on a Bruker AVANCE 400 MHz spectrometer (400 MHz for 1 H NMR and 100 MHz for 13 C NMR) at 24 • C (297 K). The samples were dissolved in 500 µL of methanol-d 4 using a 5.0 mm Wilmad-LabGlass™ NMR tube. The residual solvent signals, methanol-d 4 at δ H 3.31/δ C 49.0 ppm and tetramethylsilane (TMS), were served as the internal standard.

In-Vitro α-Glucosidase Inhibitory Assay
The α-glucosidase assay was performed as described by Liu et al. [21] with slight modification. The reaction mixture consisting of 50 µL of potassium phosphate buffer (PBS, 0.1 M, pH 6.8) and 20 µL of α-glucosidase (0.5 U/mL) was pre-incubated with 20 µL of the test sample at 37 • C for 15 min in a 96-well plate. The mixture was added 20 µL p-NPG (1 mM) and incubated again at 37 • C for 15 min. A total of 50 µL of Na 2 CO 3 (0.2 M) was added to terminate the enzymatic activity. The absorbance value of each well was measured immediately using a microplate reader at 405 nm. Acarbose was used as a positive control. All glycosides were dissolved in 50% DMSO, while a crude extract was dissolved in 100% DMSO. The experiment was performed in triplicate. The enzyme inhibition rate was calculated as follows: where OD sample represents the absorbance of PBS + enzyme + sample + p-NPG; OD sample blank represents the absorbance of PBS + sample; OD control represents the absorbance of PBS + enzyme + solvent + p-NPG; OD blank represents the absorbance of PBS + solvent. The inhibitory activity is expressed as IC 50 (half minimal inhibitory concentration) value. Data were normalized, and the IC 50 values were calculated using GraphPad Prism 9.2.0.

Evaluation of Virucidal Activity
Virucidal activity of glycosides was evaluated against influenza A virus H1N1 by the American Society for Testing and Materials (ASTM) method No. ASTM E1053-20 [22]. Prior to the virucidal assay, cytotoxicity of glycosides in Madin-Darby Canine Kidney (MDCK) cells was preliminarily examined at the concentrations of 500, 250.0, 125.0, 62.5, 31.3, 15.6, and 7.80 µg/mL by the MTT assay. The concentrations at which the compounds show more than 70% cell viability are considered non-toxic concentrations. Glycosides were tested for virucidal activity at their non-toxic concentrations. Influenza A virus H1N1 was cultured on MDCK host cells. The virus was exposed to glycosides for 10 min, and then it was neutralized and filtered. The filtrate was subjected to a serial 10-fold virus dilutions and applied in quadruplicate to 96-well plates containing MDCK monolayer. Cells in an individual well were observed for cytopathic effects. Amounts of infectious virus were quantified by the Spearman-Karber method. A total of 0.21% Sodium hypochlorite was used as a positive virucidal agent, which has a Log reduction of >4.4, accounting for 99.99% of the reduction in the virus [23].

Profile of Compounds in a Crude Extract of C. Grandis Revealed by 1 H NMR Spectrum and LC-MS/MS Analysis, and GNPS Molecular Networking of Flavonoid Glycosides
1 H NMR spectrum (in CD 3 OD) of a crude extract of C. grandis leaves showed signals of flavonoid at the chemical shifts (δ H ) 6-8 ppm, which belong to flavonoids, kaempferol, and quercetin (Supplementary Figure S1). LC-MS analysis of a crude extract of C. grandis was performed, and its TIC chromatogram is shown in Supplementary Figure S2. LC-MS/MS analysis showed 32 tentatively identified compounds in a methanolic extract of C. grandis obtained from LC-MS/MS analysis (Table 1) (Table 1). In addition, we also performed analysis of the crude extract using LC-MS/MS molecular networking approach by employing the GNPS system to create molecular networks. All converted MS/MS data were used to generate network nodes in which corresponding to a consensus MS/MS spectrum having similar precursor mass. The network nodes were connected via edges based on the cosine similarity score where the high score reflects the close relatedness between two nodes.
The GNPS molecular networking was performed for a crude leaf extract of C. grandis using a positive ion mode (Supplementary Materials, Figure S3). Initially, a commercially available quercetin (8) was used as a standard compound for the GNPS molecular networking of a crude leaf extract, leading to the networking generation shown in Figure 2. Quercetin (8) had the networking with flavonoids 1, 2, 5, and 6 ( Figure 2). We then performed the isolation of C. grandis extract, and obtained compounds 1-3 as major constituents. Subsequently, we used flavonoids 1-3 as standard compounds for the GNPS molecular networking, and the networking is depicted in Figure 2 Table 1, the GNPS molecular networking provides further information on the presence of some flavonoid glycosides, and it confirms the existence of flavonoids in a crude extract by cosine score. The information from this molecular networking (Figure 2) led to the isolation of compounds 1, 2, 3, 5, and 6, whose structures were characterized by analysis of NMR data. It is worth mentioning that there are a number of clusters of the GNPS molecular networking (Supplementary Figure S3). However, other clusters could not be analyzed because there were no authentic standard compounds available. Moreover, 1 H NMR spectrum indicated the presence of flavonoids as major compounds in C. grandis leaf extract; with this information, we focused only on the networking of flavonoids.

Isolation and Characterization of Flavonoid Glycosides
Flavonoids are known to have α-glucosidase inhibitory, antimicrobial, and antiviral activities [24][25][26], and they are also used as natural additives [27]. As 1 H NMR spectrum and GNPS molecular networking suggested the presence of flavonoids in an extract of C. grandis, isolation of flavonoids was performed, giving seven flavonoid glycosides 1-7 ( Figure 1). 1 H, 13 C NMR, and MS spectra of compounds 1-7 are in the Supplementary Figure S4-S26. Rutin (1), a yellow amorphous powder, had the molecular formula of C27H30O16 as deduced from high-resolution electrospray ionization mass spectrometry (HRESI-MS). The structure of rutin (1) was confirmed by analysis of 1D and 2D NMR spectra, whose data were identical to those reported in the literature [28]. Kaempferol 3-O-rutinoside (2) or nicotiflorin was obtained as yellow amorphous solid with a molecular formula of C27H30O15 (from HRESI-MS). Analysis of NMR spectra established the structure of glycoside 2, and their NMR data were in good agreement with published values [29]. Kaempferol 3-O-robinobioside (3) or kaempferol 3-O-alpha-L-rhamnopyranosyl-(1→6)beta-D-galactopyranoside was obtained as yellow amorphous solid with the molecular formula of C27H30O15 as inferred from HRESI-MS. NMR data of glycoside 3 were in good agreement with those reported in the literature [30]. Quercetin 3-O-robinobioside (4) had a molecular formula of C27H30O16 as deduced from HRESI-MS. Hassan and co-workers first reported the structure of glycoside 4; however, they obtained glycoside 4 as a mixture with rutin (1) [31]. In this work, we also obtained the same mixture of glycosides 4 and 1 with the proportion ratio of 1.00:0.79. On the reversed phase HPLC chromatogram, a mixture of 4 and 1 exhibited only a single peak, suggesting that it is an inseparable mixture. It is worth mentioning that the first sugar unit attached to a flavonoid skeleton is different among glycosides 1-4; compounds 1 and 2 have glucose, while compounds 3 and 4 have galactose (Figure 1). Glycoside 5 was obtained as yellow amorphous solid with the molecular formula of C32H38O20 (by HRESI-MS). The structure of 5 was established by analysis of spectroscopic data, and it was identified as quercetin 3-O-β-D-apiofuranosyl-(1→2)-[α-

Isolation and Characterization of Flavonoid Glycosides
Flavonoids are known to have α-glucosidase inhibitory, antimicrobial, and antiviral activities [24][25][26], and they are also used as natural additives [27]. As 1 H NMR spectrum and GNPS molecular networking suggested the presence of flavonoids in an extract of C. grandis, isolation of flavonoids was performed, giving seven flavonoid glycosides 1-7 ( Figure 1). 1 H, 13 C NMR, and MS spectra of compounds 1-7 are in the Supplementary Figure S4-S26. Rutin (1), a yellow amorphous powder, had the molecular formula of C 27 H 30 O 16 as deduced from high-resolution electrospray ionization mass spectrometry (HRESI-MS). The structure of rutin (1) was confirmed by analysis of 1D and 2D NMR spectra, whose data were identical to those reported in the literature [28]. Kaempferol 3-O-rutinoside (2) or nicotiflorin was obtained as yellow amorphous solid with a molecular formula of C 27 H 30 O 15 (from HRESI-MS). Analysis of NMR spectra established the structure of glycoside 2, and their NMR data were in good agreement with published values [29]. Kaempferol 3-O-robinobioside (3) or kaempferol 3-O-alpha-L-rhamnopyranosyl-(1→6)beta-D-galactopyranoside was obtained as yellow amorphous solid with the molecular formula of C 27 H 30 O 15 as inferred from HRESI-MS. NMR data of glycoside 3 were in good agreement with those reported in the literature [30]. Quercetin 3-O-robinobioside (4) had a molecular formula of C 27 H 30 O 16 as deduced from HRESI-MS. Hassan and co-workers first reported the structure of glycoside 4; however, they obtained glycoside 4 as a mixture with rutin (1) [31]. In this work, we also obtained the same mixture of glycosides 4 and 1 with the proportion ratio of 1.00:0.79. On the reversed phase HPLC chromatogram, a mixture of 4 and 1 exhibited only a single peak, suggesting that it is an inseparable mixture. It is worth mentioning that the first sugar unit attached to a flavonoid skeleton is different among glycosides 1-4; compounds 1 and 2 have glucose, while compounds 3 and 4 have galactose (Figure 1). Glycoside 5 was obtained as yellow amorphous solid with the molecular formula of C 32 H 38 O 20 (by HRESI-MS). The structure of 5 was established by analysis of spectroscopic data, and it was identified as quercetin 3-O-β-D-apiofuranosyl-(1→2)-[α-Lrhamnopyranosyl-(1→6)]-β-D-glucopyranoside or CTN-986 or apiorutin, whose NMR data were identical to those of published values [31][32][33]. Glycoside 6 was obtained as yellow amorphous solid with the molecular formula of C 32 H 38 O 19 (by HRESI-MS), and it was identified as kaempferol 3-O-β-D-apiofuranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→6)]β-D-glucopyranoside. Spectroscopic data of 6 were identical to those reported in the literature [32]. Glycoside 7 was obtained as yellow amorphous solid, and it had the same molecular formula of C 32 H 38 O 19 (by HRESI-MS) as that of compound 6. Compound 7 was identified as kaempferol 3-O-β-D-apiofuranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→6)]-β-D-galactopyranoside, and its NMR data were in good agreement with those reported in the literature [33]. NMR data of glycosides 1-7 are in the Supplementary Tables S1 and S2.
Recently, rutin (1) and kaempferol 3-O-rutinoside (2) were reported to be the constituents in C. grandis by LC-ESI-MS/MS analysis [2,17]. It is worth mentioning that kaempferol 3-O-rutinoside (2) has the same molecular weight as that of kaempferol 3-Orobinobioside (3). Compound 2 has glucose, but glycoside 3 has galactose, and both sugars has the same molecular weight; therefore, LC-MS/MS technique hardly distinguishes between the glycosides 2 and 3. For this reason, when analyzing by LC-MS/MS, only glycosides 1 and 2 with glucose in the molecules were proposed to be the constituents in C. grandis [2,17]. Again, glycoside 6 has the same molecular weight as that of compound 7, and the difference is the presence of glucose in glycoside 6 and galactose in 7. Therefore, the LC-MS/MS method hardly distinguishes between compounds 6 and 7. The present work is the first report on the isolation and characterization of glycosides 1-7 from C. grandis. Moreover, this work reveals that there are two closely related sugars, glucose or galactose, attached to the position 3 of a flavonoid backbone, thus generating the chemical diversity in C. grandis. Increase in chemical diversity of flavonoid glycosides would lead to their diverse biological activities. Unexpectedly, the glycosides with a sugar apiose, i.e., compounds 5-7, were isolated from a crude extract of C. grandis leaves. Among them, 3-O-β-D-apiofuranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→6)]-β-D-glucopyranoside (5), also known as CTN-986, previously isolated from cottonseeds, was found to exhibit antidepressant and cytoprotective effects [34]; it could enhance the proliferation of hippocampal neural progenitor cells, possibly via the 5-HT1A receptor [35]. A study on pharmacokinetics and tissue distribution revealed that CTN986 could be absorbed and distributed into tissues in the mice model [36,37]. Glycosides with a sugar apiose have diverse biological activities, and the roles of apiosylated compounds in plants are considered a challenging open research area [38]. Therefore, apiose-containing compounds have recently been received attention, both in biosynthesis and chemical synthesis [39,40].

α-Glucosidase Inhibitory Activity of the Isolated Flavonoid Glycosides
There have been a number of reports on the antidiabetic activity of C. grandis crude extracts, as mentioned earlier in the introduction. However, the compounds responsible for the antidiabetic activity have rarely been isolated and characterized. In the present work, we also found that a crude extract of C. grandis leaves exhibited α-glucosidase inhibitory activity with IC 50 value of 1.24 ± 0.07 mg/mL (Supplementary Figure S27). Seven glycosides 1-7 isolated from C. grandis leaves were evaluated for α-glucosidase inhibitory activity (Table 2). Acarbose, the α-glucosidase inhibitor drug, was used as a standard compound. Curves for dose-dependent α-glucosidase inhibitory activity of acarbose and glycosides are in Figure 3. As shown in Table 2 (Table 2). It is worth mentioning that the activity (IC 50 of 195.4 µM) of glycoside 3 with galactose was slightly more active than that (IC 50 of 235.8 µM) of its corresponding isomer 2 attached with glucose ( Table 2). Among glycosides 1-7, rutin (1) and kaempferol 3-O-rutinoside (2) isolated form leaves of Morus atropurpurea were previously reported to have α-glucosidase inhibitory activity [41]. Compounds 3-7 have never been reported as α-glucosidase inhibitors so far. The present work characterizes the structures of αglucosidase inhibitors in C. grandis for the first time. However, there may be other classes of compounds in this plant acting as α-glucosidase inhibitors. All compounds isolated from C. grandis leaves exhibited better α-glucosidase inhibitory activity than acarbose, and this work supports the recent clinical trial on C. grandis leaves for the treatment of type 2 diabetes patients [1].  Table 2). It is worth mentioning that the activity (IC50 of 195.4 µM) of glycoside 3 with galactose was slightly more active than that (IC50 of 235.8 µM) of its corresponding isomer 2 attached with glucose ( Table 2). Among glycosides 1-7, rutin (1) and kaempferol 3-O-rutinoside (2) isolated form leaves of Morus atropurpurea were previously reported to have α-glucosidase inhibitory activity [41]. Compounds 3-7 have never been reported as α-glucosidase inhibitors so far. The present work characterizes the structures of α-glucosidase inhibitors in C. grandis for the first time. However, there may be other classes of compounds in this plant acting as α-glucosidase inhibitors. All compounds isolated from C. grandis leaves exhibited better α-glucosidase inhibitory activity than acarbose, and this work supports the recent clinical trial on C. grandis leaves for the treatment of type 2 diabetes patients [1].

Virucidal Activity of the Isolated Flavonoid Glycosides
Six flavonoid glycosides 1-6 were evaluated for virucidal activity against influenza A virus H1N1 ( Table 3). A small amount of compound 7 was obtained, which was not sufficient for virucidal activity testing. Compounds 1-3 had cell viability more than 70% at the concentrations of 125.0 µ g/mL or at lower concentrations, while a mixture of 4 and 1, compounds 5-6, had cell viability of more than 70% at the concentrations of 500.0 µ g/mL or at lower concentrations ( Table 3). Virucidal activity of the isolated glycosides were reported at the concentrations with cell viability more than 70%. As shown in Table 3, at the concentration of 125.0 µ g/mL, glycosides 1-3 showed virucidal activity with Log reduction of 1.80-1.95, accounting for 99% reduction of virus. Compound 3 at lower concentrations of 31.3 and 15.6 µg/mL retained its virucidal potency with Log reduction around 1.9 that could reduce 99% of virus. A mixture of 4 and 1, compounds 5 and 6, at the concentrations of 500.0 and 250.0 µg/mL exhibited virucidal activity with Log reduction of 1.93-2.41, accounting for a 99% reduction of virus, and these glycosides could retain virucidal potency with the Log reductions of 1.8-1.9 at lower concentrations, even at the lowest concentration tested at 15.6 µ g/mL (Table 3). Overall, flavonoid glycosides in leaves of C. grandis exhibited moderate virucidal activity with 99% reduction of virus, when compared to a positive virucidal agent, 0.21% sodium hypochlorite, which is a common household disinfectant that could reduce 99.99% of virus (Log reduction of >4.4). Rutin (1) was previously reported to act as a potent hepatitis C virus entry inhibitor [42], and recently, an in silico study revealed that it could bind with the SARS-CoV-2 (COVID-19) main protease [43]. Kempferol 3-O-rutinoside (2) was reported to possess strong inhibition towards dengue virus serine protease [44]. Glycosides 1-3 were reported to exhibit potent antiviral activity against herpes simplex virus-1 and -2 [45]. Recently, flavanone glycosides from Dracocephalum spp. were reported to exhibit virucidal activity against feline calicivirus [46]. The present work underscores the importance of glycosides as sources of natural antiviral and virucidal agents.

Virucidal Activity of the Isolated Flavonoid Glycosides
Six flavonoid glycosides 1-6 were evaluated for virucidal activity against influenza A virus H1N1 (Table 3). A small amount of compound 7 was obtained, which was not sufficient for virucidal activity testing. Compounds 1-3 had cell viability more than 70% at the concentrations of 125.0 µg/mL or at lower concentrations, while a mixture of 4 and 1, compounds 5-6, had cell viability of more than 70% at the concentrations of 500.0 µg/mL or at lower concentrations (Table 3). Virucidal activity of the isolated glycosides were reported at the concentrations with cell viability more than 70%. As shown in Table 3, at the concentration of 125.0 µg/mL, glycosides 1-3 showed virucidal activity with Log reduction of 1.80-1.95, accounting for 99% reduction of virus. Compound 3 at lower concentrations of 31.3 and 15.6 µg/mL retained its virucidal potency with Log reduction around 1.9 that could reduce 99% of virus. A mixture of 4 and 1, compounds 5 and 6, at the concentrations of 500.0 and 250.0 µg/mL exhibited virucidal activity with Log reduction of 1.93-2.41, accounting for a 99% reduction of virus, and these glycosides could retain virucidal potency with the Log reductions of 1.8-1.9 at lower concentrations, even at the lowest concentration tested at 15.6 µg/mL (Table 3). Overall, flavonoid glycosides in leaves of C. grandis exhibited moderate virucidal activity with 99% reduction of virus, when compared to a positive virucidal agent, 0.21% sodium hypochlorite, which is a common household disinfectant that could reduce 99.99% of virus (Log reduction of >4.4). Rutin (1) was previously reported to act as a potent hepatitis C virus entry inhibitor [42], and recently, an in silico study revealed that it could bind with the SARS-CoV-2 (COVID-19) main protease [43]. Kempferol 3-O-rutinoside (2) was reported to possess strong inhibition towards dengue virus serine protease [44]. Glycosides 1-3 were reported to exhibit potent antiviral activity against herpes simplex virus-1 and -2 [45]. Recently, flavanone glycosides from Dracocephalum spp. were reported to exhibit virucidal activity against feline calicivirus [46]. The present work underscores the importance of glycosides as sources of natural antiviral and virucidal agents.

Conclusions
In the present study, GNPS molecular networking was used to guide chemical investigation of compounds in a crude extract of C. grandis leaves. Seven flavonoid glycosides 1-7 were isolated from C. grandis extract, and they were found to be potent α-glucosidase inhibitors, which were even more potent than acarbose, an antidiabetic drug. This is the first report on the systematic isolation and identification of α-glucosidase inhibitors in C. grandis and on the α-glucosidase inhibitory activity of flavonoid glycosides 3-7. The finding of potent α-glucosidase inhibitors in C. grandis leaves supports the recent clinical trial on the use of C. grandis leaves for the treatment of patients with type 2 diabetes mellitus, and it revealed that C. grandis remarkably improved a glycemic profile of patients [1]. Since C. grandis leaves are used as vegetables in many countries, they may partly contribute the benefits for people with type 2 diabetes mellitus. Moreover, glycosides 1-6 were also found to have moderate virucidal activity against influenza A virus H1N1. Foodborne viruses, for example hepatitis A and hepatitis E viruses, have caused problems in the food industry, thus causing a serious problem for public health [47]. Inactivation of foodborne viruses by natural products is one of the approaches for the control of these viruses [48,49]. This work provides the information about virucidal flavonoid glycosides in C. grandis, which is used as a common food ingredient in many Asian dishes.