Redox-Active 1,4-Pyrazine-Bis(thiosemicarbazone) Ligands

Tetranuclear complexes [M4(L)4] with M = Ni(II) or Zn(II), with a [2 × 2] grid-type structure, were assembled in good yields and purity from the easily accessible but unprecedented pyrazine-bridged bis(thiosemicarbazone) protoligands (ligand precursors) H2L (1,4-pyrazine-2,5bis(R-carbaldehyde-thiosemicarbazone); R = Me, Et, iPr, or Ph). The complexes were characterised in solution by NMR, MS, IR, and UV-Vis absorption spectroscopy and (spectro)electrochemical methods. HR-MS spectra unequivocally reveal that the tetranuclear species are very stable in solution and any measurements represent these species. Only at higher temperatures (fragmentation in solution: MS and in the solid: TG-DTA) or upon the addition of protons (acidic UV-Vis titrations) can the tetrameric entities be decomposed. Single crystal XRD measurement remained preliminary. Rapid loss of co-crystallised solvent molecules within the [2 × 2] grid-type structures resulted in crystals of very poor quality, but the results were qualitatively in line with spectroscopy, electrochemistry, and quantum chemical (DFT) calculations. IR and NMR spectroscopy point clearly to a thiolate coordination of dianionic (deprotonated) ligands. The electrochemistry reveals four electronically coupled and reversible one-electron reductions centred largely at the pyrazine bridges. EPR and UV-Vis spectroelectrochemical measurements in combination with DFT calculation support the assignment.

Remarkably, among the arene bis(thiosemicarbazones) ligands, there are only a few in which bifunctional heterocyclic arene units directly connect two (M-arene-M) or more metals.
1,4-Pyrazine has previously shown to be a very suitable ligand to bridge two metal centres and provide effective electronic coupling between the two atoms [39][40][41][42]. The Creutz-Taube ion Scheme 2. The 1,4-pyrazine-bridged bis(thiosemicarbazone) protoligands (ligand precursor) H 2 L R with R = Me (L Me ), Et (L Et ), i Pr (L iPr ), or Ph (L Ph ) used in this study (A); the dianionic ligands (L R ) 2− bridging two metals (B); the assumed tetrameric products obtained in this study (C).
In this contribution, we report the synthesis of four new 1,4-pyrazine-bridged bis(thiosemicarbazones) H 2 L R and their tetranuclear complexes [M 4 (L R ) 4 ] (M = Ni or Zn). Detailed spectroscopic and electrochemical characterisation reveals that a number of specific topics render these new metallosquares remarkable.
In this contribution, we report the synthesis of four new 1,4-pyrazine-bridged bis(thiosemicarbazones) H2L R and their tetranuclear complexes [M4(L R )4] (M = Ni or Zn). Detailed spectroscopic and electrochemical characterisation reveals that a number of specific topics render these new metallosquares remarkable.

Synthesis and Characterisation
Synthesis of the protoligands (protonated ligand precursors) H2L R . For the synthesis of the pyrazine-2,5-carbaldehydes (R = Me, Et, i Pr, or Ph), we modified a literature procedure [61] converting aldehydes to the corresponding radicals by a Fenton type reaction (Scheme 3 left), and 1,4-pyrazine was then added (details in the Supplementary Materials). After formation the pyrazine-2,5-carbaldehydes precipitate from the reaction, yields were only moderate (~35%). The yellow pyrazine-2,5-carbaldehydes were reacted with thiosemicarbazide (Scheme 3, right) to produce the bis(thiosemicarbazone) protoligands H2L R in good yield (63-90%). Synthesis and analysis of the complexes. The protoligands H2L R were dissolved in DMA (N,N-dimethylacetamide) and KOH dissolved in EtOH was added. This mixture was layered carefully using a 0.01 M solution of [Zn(acac)2] (acac − = acetylacetonate) dissolved in MeCN. From many solvents, we tried DMA was best to prevent any precipitation during this procedure. Careful crystallisation over one or two weeks yielded red microcrystalline materials (details in the experimental section). Corresponding reactions using Ni(BF4)2 yielded dark blue materials. Yields for the eight new compounds were good (45-75%), and the solubility of the products in organic solvents was generally low; they were air-and moisture-stable. Elemental analysis gave M:L ratios of 1:1 in the products, and the anionic thiolate form of the ligand was confirmed by IR spectroscopy. For example, the azoN-H function disappeared, and strong vibrations in the range 1200 to 1400 cm −1 represent the increasing double bond character in the -N=C(S)(NH2) unit of the deprotonated ligand [9]. Consequently, the C=S stretching mode shifts from about 840 cm −1 to values ranging from 730 to 780 cm −1 , indicative of the coordinated thiolate form in the complexes. NMR spectroscopy was carried out on the protoligands H2L R but failed on most of the Zn(II) complexes due to low solubility. Synthesis and analysis of the complexes. The protoligands H 2 L R were dissolved in DMA (N,N-dimethylacetamide) and KOH dissolved in EtOH was added. This mixture was layered carefully using a 0.01 M solution of [Zn(acac) 2 ] (acac − = acetylacetonate) dissolved in MeCN. From many solvents, we tried DMA was best to prevent any precipitation during this procedure. Careful crystallisation over one or two weeks yielded red microcrystalline materials (details in the experimental section). Corresponding reactions using Ni(BF 4 ) 2 yielded dark blue materials. Yields for the eight new compounds were good (45-75%), and the solubility of the products in organic solvents was generally low; they were air-and moisture-stable. Elemental analysis gave M:L ratios of 1:1 in the products, and the anionic thiolate form of the ligand was confirmed by IR spectroscopy. For example, the azoN-H function disappeared, and strong vibrations in the range 1200 to 1400 cm −1 represent the increasing double bond character in the -N=C(S)(NH 2 ) unit of the deprotonated ligand [9]. Consequently, the C=S stretching mode shifts from about 840 cm −1 to values ranging from 730 to 780 cm −1 , indicative of the coordinated thiolate form in the complexes. NMR spectroscopy was carried out on the protoligands H 2 L R but failed on most of the Zn(II) complexes due to low solubility. For the Ni(II) derivatives, the paramagnetic behaviour precluded meaningful NMR experiments (figures in the Supplementary Materials).
Mass spectrometry unequivocally revealed the presence of tetranuclear complexes in solutions of [M 4

Properties of the Solids
TG-DTA measurements on the bulk solid materials are in line with the tetrameric character and reveal up to two molecules DMA and in all cases four molecules H 2 O adherent to the tetrameric units from recrystallisation (figures in the Supplementary Materials) in line with the elemental analyses (see Experimental Section). Up to about 250 • C, the volatile H 2 O and DMA molecules evaporate, followed by an exothermic decomposition cleaving the NH 2 C(S)N fragments, which can be concluded from a mass-loss of about 30-35%. Crystal and molecular structures could not be obtained yet. From the complexes, [M 4 (L R ) 4 ] single crystals were obtained and submitted to X-ray diffraction experiments. Unfortunately, most of the structure solutions were of low quality. High R int values point to low crystal quality, which is probably due to solvent molecules in the lattice. The best obtained structure solution could be carried out for [Ni 4 (L iPr ) 4 ], details on the structure solution can be found in the Supplementary Materials.
The tetrameric molecular structure revealed by Figure 3 is fully in line with HR-MS, further spectroscopies, electrochemistry, and quantum chemical calculations (see later), which makes us confident that, although the structure solution is very poor, the structure is essentially correct.

Properties of the Solids
TG-DTA measurements on the bulk solid materials are in line with the tetrameric character and reveal up to two molecules DMA and in all cases four molecules H2O adherent to the tetrameric units from recrystallisation (figures in the supplementary materials) in line with the elemental analyses (see Experimental Section). Up to about 250 °C, the volatile H2O and DMA molecules evaporate, followed by an exothermic decomposition cleaving the NH2C(S)N fragments, which can be concluded from a mass-loss of about 30-35%. Crystal and molecular structures could not be obtained yet. From the complexes, [M4(L R )4] single crystals were obtained and submitted to X-ray diffraction experiments. Unfortunately, most of the structure solutions were of low quality. High Rint values point to low crystal quality, which is probably due to solvent molecules in the lattice. The best obtained structure solution could be carried out for [Ni4(L iPr )4], details on the structure solution can be found in the supplementary materials.
The tetrameric molecular structure revealed by Figure 3 is fully in line with HR-MS, further spectroscopies, electrochemistry, and quantum chemical calculations (see later), which makes us confident that, although the structure solution is very poor, the structure is essentially correct.

UV-Vis Absorption Spectroscopy
The coordination of the protoligands H2L R to Ni(II) and Zn(II) under deprotonation can be also traced in the UV-Vis absorption spectra. While the protoligands H2L R having a -C(R)=N-NH-C(=S)NH2 configuration exhibit intense long-wavelength absorption maxima up to about 400 nm ( Table S4 in  These quite intense long-wavelength bands (ε > 20,000 M −1 •cm −1 ) are also in line with the red colour of the Zn(II) containing materials. The maxima of the even more intense (ε > 40,000 M −1 •cm −1 ) long-wavelength bands of the Ni(II) complexes exceed 600 nm ( Figure 4) in line with the blue colour of the materials. Due to their similar appearance, the long-wavelength bands in both Zn(II) and Ni(II) complexes were assigned to intra-ligand absorptions. For the Ni(II) complexes additional absorptions can be observed as very long-wavelength shoulders around 700 nm, they might be assigned to nickel(d) to pyrazine(π*) metal-to-ligand charge transfer (MLCT) transitions.

UV-Vis Absorption Spectroscopy
The coordination of the protoligands H 2 L R to Ni(II) and Zn(II) under deprotonation can be also traced in the UV-Vis absorption spectra. While the protoligands H 2 L R having a -C(R)=N-NH-C(=S)NH 2 configuration exhibit intense long-wavelength absorption maxima up to about 400 nm ( Table S4 in

Electrochemistry
The electrochemical behaviour of the protoligands and the complexes was studied by cyclic voltammetry (CV) and square-wave voltammetry (SQW). Figure 5 shows examples, Table 1 summarises the redox potentials. The tetranuclear complexes exhibit four consecutive one-electron reduction steps in the range of −1.4 to −2 V, which are completely reversible on the timescale of the experiment. In the same range, the protoligands H2L R show one reversible reduction. Thus, the pyrazine moiety seems largely to be the target for these reduction processes [33,39,40,42], although some variation of the potentials stems from the R substituents. In the tetranuclear complexes, we thus assign the first four waves to successive one-electron reduction of the four pyrazine moieties. We assume that the second reduction occurs to the pyrazine moiety opposite to the firstly reduced pyrazine group, similar to what has been described for the four Fe(II)/Fe(III) oxidation processes in the [2 × 2] grid [Fe4(L)4] (HL = 4-methyl-3,5-bis{6-(2,2′-bipyridyl)} pyrazole [51]. The differences between first and second reduction potentials (∆E) as well as between the third and fourth reduction potentials are around 110 mV, while the second and third reduction potentials differ about 300 mV. Remarkably, this is regardless of the incorporated metal. Unfortunately, the irreversibility of the second reductions for the protoligands H2L R precludes the exact calculation of the differences ∆E, but the values surely exceed 200 mV (Table 1).  4 ] complex can be reconstituted. The larger extent of protonation, e.g., to yield [M 4 (HL R ) 3 (H 2 L R )] 5+ probably leads to an irreversible disintegration. Unfortunately, the same tests in water, allowing for a quantitative description using the pK A value of HOAc and measuring the basicity of [M 4 (L R ) 4 ], were prevented by the poor solubility of the compounds in water.

Electrochemistry
The electrochemical behaviour of the protoligands and the complexes was studied by cyclic voltammetry (CV) and square-wave voltammetry (SQW). Figure 5 shows examples, Table 1 summarises the redox potentials. The tetranuclear complexes exhibit four consecutive one-electron reduction steps in the range of −1.4 to −2 V, which are completely reversible on the timescale of the experiment. In the same range, the protoligands H 2 L R show one reversible reduction. Thus, the pyrazine moiety seems largely to be the target for these reduction processes [33,39,40,42], although some variation of the potentials stems from the R substituents. In the tetranuclear complexes, we thus assign the first four waves to successive one-electron reduction of the four pyrazine moieties. We assume that the second reduction occurs to the pyrazine moiety opposite to the firstly reduced pyrazine group, similar to what has been described for the four Fe(II)/Fe(III) oxidation processes in the [2 × 2] grid [Fe 4 (L) 4 ] (HL = 4-methyl-3,5-bis{6-(2,2 -bipyridyl)} pyrazole [51]. The differences between first and second reduction potentials (∆E) as well as between the third and fourth reduction potentials are around 110 mV, while the second and third reduction potentials differ about 300 mV. Remarkably, this is regardless of the incorporated metal. Unfortunately, the irreversibility of the second reductions for the protoligands H 2 L R precludes the exact calculation of the differences ∆E, but the values surely exceed 200 mV (Table 1). Inorganics 2018, 6, x FOR PEER REVIEW 7 of 18  We can conclude that, in the [2 × 2] grid arrangement of the tetranuclear complexes [M4(L R )4], the four pyrazine moieties are electronically connected, allowing one to determine four separate waves and thus the observation of three mixed-valent states. The stability constants KC = 10 (ΔE/59mV) [40,42] are about 10 2 for the first product of reduction [M4(L R )3(L R• )] •− and about 10 5 for [M4(L R )2(L R• )2] 2− . A metal-mediated electronic coupling through empty d orbitals can be ruled out since the values for Ni(II) having an empty dx 2 −y 2 orbital and for Zn(II) having no empty d orbital are much the same. Instead, the four crystallographically and spectroscopically identical pyrazine moieties are electronically connected through spacial proximity, as has been reported for a number of rectangular [{Re(CO)3}4(μ-X)2(μ-L)2] (X = thiolate, alkoxide [67]; X/2 = 2,2′-bisbenzimidazolate [68] or octahydro-2,2′-bipyrimidine) [69] complexes with heteroaromatic bridging ligands such as 1,4-pyrazine or 4,4′-bipyridine. Depending on the plane-to-plane distance, the potential splitting ΔE for the pyrazine systems lie between 200 (larger than 5.5 Å) [68,69] and 450 mV (around 4 Å) [67]. Interestingly, even for the weakly coupled system (X/2 = 2,2′-bisbenzimidazolate) [68] with a 250 mV separation a weak intervalence charge transfer (IVCT) absorption was observed at 5350 cm −1 (1870 nm; ε = 150 M −1 •cm −1 ) for the first reduced mixed-valent state, for stronger coupled systems having shorter ligand-to-ligand distances, these bands can be quite pronounced [67,68,70].
In the mononuclear Ga(III) complexes [Ga(L) 2 ] + containing two neighbouring pyrazine thiosemicarbazonate ligands, two ligand-centred one-electron reductions were observed at about −1.3 V and −1.5 V, respectively, with separations ∆E~270 mV [33]. For the Fe(III) derivatives [Fe(L) 2 ] + , the reductions lie at around −1.9 V and −2.3 V, respectively, revealing larger separation ∆E~420 mV. Importantly, a first reduction of the [Fe(L) 2 ] + complexes can be assigned to a Fe(II)/Fe(III) couple, thus these ligand-centred reductions occur to neutral Fe(II) complexes [Fe(L) 2 ] [33]. For these Ga and Fe complexes, third reduction waves have been observed at potentials of about −2.2 V (Ga) and −2.8 V (Fe), respectively, and a fourth reduction has been observed at around −2.5 V for the Ga derivatives. The third waves lie over 600 mV lower than the second, indicative of the second reduction of a pyrazine moiety [39,40]. For these complexes, the first two reductions occur thus successively to the two metal-connected pyrazine-thiosemicarbazone ligands, the third and fourth reduction waves represent the second reduction of these units [33]. The larger ∆E observed for the Fe(II) d 6 low spin systems [33] points to an efficient electronic coupling of the two pyrazine moieties through empty metal d orbitals. However, for Ga(III) d 10 , such a mechanism can be ruled out. ∆E is smaller but still appreciable. Thus, also here, in line with our findings, a through-space electronic interaction can be postulated for the two pyrazine units.
The second reduction of the four pyrazine moieties in our complexes [M 4  There are a number of irreversible oxidation waves at around +0.5 V both in the protoligands H 2 L R and the Zn complexes, which were assigned to oxidations at the thiol(ate) group of the thiosemicarbazone moiety. For the Ni(II) complexes, additional Ni(II)/Ni(III) couples were observed at even lower potentials. Due to the irreversible nature of all of these processes, the assignment remains preliminary.

Spectroelectrochemical UV-Vis Absorption and EPR Spectroscopy
UV-Vis absorption spectra were recorded during cathodic electrolysis of the two complexes [M 4 (L iPr ) 4 ] (M = Ni or Zn). For M = Ni, Figure 6 shows a continuous change of the spectra during the first two reduction steps (left part) with no evidence for a defined mixed-valent species [Ni 4 (L iPr ) 4 ] •− , especially no intervalence charge transfer (IVCT) band [39,40,42,67,68,70], in line with the far higher stability constant K C of 10 5 for [M 4 (L iPr ) 2 (L iPr• ) 2 ] 2− compared with 10 2 for [M 4 (L iPr ) 3 (L iPr• )] •− . At −1.9 V, the observed process (first and second reduction), which goes along with a blue-shift of the two dominating bands at 605 (to 565 nm) and 392 (to 371 nm) and the appearance of a weaker double maximum at 694 and 635 nm is completed (Figure 6a).  (Fe), respectively, and a fourth reduction has been observed at around −2.5 V for the Ga derivatives. The third waves lie over 600 mV lower than the second, indicative of the second reduction of a pyrazine moiety [39,40]. For these complexes, the first two reductions occur thus successively to the two metal-connected pyrazine-thiosemicarbazone ligands, the third and fourth reduction waves represent the second reduction of these units [33]. The larger ΔE observed for the Fe(II) d 6 low spin systems [33] points to an efficient electronic coupling of the two pyrazine moieties through empty metal d orbitals. However, for Ga(III) d 10 , such a mechanism can be ruled out. ΔE is smaller but still appreciable. Thus, also here, in line with our findings, a through-space electronic interaction can be postulated for the two pyrazine units. The second reduction of the four pyrazine moieties in our complexes [M4(L R )4] 4− →[M4(L R )4] 5−/6−/7−/8− are expected to occur at potentials approximately 600-700 mV lower (more negative) and were not detected unequivocally in our experiments due to the overlap with solvent and water (co-crystallised) discharge waves.
There are a number of irreversible oxidation waves at around +0.5 V both in the protoligands H2L R and the Zn complexes, which were assigned to oxidations at the thiol(ate) group of the thiosemicarbazone moiety. For the Ni(II) complexes, additional Ni(II)/Ni(III) couples were observed at even lower potentials. Due to the irreversible nature of all of these processes, the assignment remains preliminary.

Spectroelectrochemical UV-Vis Absorption and EPR Spectroscopy
UV-Vis absorption spectra were recorded during cathodic electrolysis of the two complexes [M4(L iPr )4] (M = Ni or Zn). For M = Ni, Figure 6 shows a continuous change of the spectra during the first two reduction steps (left part) with no evidence for a defined mixed-valent species [Ni4(L iPr )4] •− , especially no intervalence charge transfer (IVCT) band [39,40,42,67,68,70], in line with the far higher stability constant KC of 10 5 for [M4(L iPr )2(L iPr• )2] 2− compared with 10 2 for [M4(L iPr )3(L iPr• )] •− . At −1.9 V, the observed process (first and second reduction), which goes along with a blue-shift of the two dominating bands at 605 (to 565 nm) and 392 (to 371 nm) and the appearance of a weaker double maximum at 694 and 635 nm is completed (Figure 6a).  Further reduction (third and fourth reduction) also results in continuous changes, such as the disappearance of the two long-wavelength weak absorptions and the red-shift of the two intense bands in the visible range (Figure 6b). Again, we found no evidence for a mixed-valent species [Ni 4 (L iPr ) 4 ] •3− , probably again due to the far higher stability of the [Ni 4 (L iPr ) 4 ] 4− state. For the Zn derivative, the behaviour is very similar (spectra in the Supplementary Materials).
A detailed look on our spectra reveals that isosbestic points are far from being perfect. This might be due to the fact that more than two species were observed, thus a weak hint for the mixed-valent species. At the same time, the spectra of the highly reduced species show some long-wavelength tailing, indicative of a precipitation of these highly charged complexes. In line with this, the full reversibility of the first four reduction processes through re-oxidation is only obtained upon long standing at 0 V. X-band EPR spectra recorded during cathodic electrolysis of [Ni 4 (L iPr ) 4 ] at potentials between −1.5 and −1.9 V (first and second reduction) gave an isotropic signal at g = 2.016 with only 45 G total spectral width ( Figure S40). This is in line with a pyrazine-centred radical complex, and no indication for a second reduced species was found.

Quantumchemical Calculations
DFT calculations were initiated to obtain insight on the energy and location of the highest occupied molecular orbital (HOMO) and the lowest unoccupied molecular orbital (LUMO) of the complexes. First, we calculated the molecular structures of the complexes in the gas phase on (RI-)BP86/def-SV(P) level and refined some of the resulting structures on (RI-)BP86/def2-TZVP level. Frequency calculations were carried out in order to check for the absence of imaginary frequencies, which confirms an energetic minimum for the geometry. The calculated minimum geometries agree quite well with the experimental structures from HR-MS in solution and with the preliminary single crystal XRD results (Tables S5 and S6).
The calculated LUMOs (Figures 7a and 8a) are largely centred on the pyrazine units in line with the EPR results but also the CNNCN chain of the thiosemicarbazonate contributes markedly, in line with the dependency of the reduction potentials on the R substituents. Importantly, there was no indication of an electronic coupling of the four pyrazine units, in line with the assumed electrostatic nature of the observed splitting of the four reduction waves. Further reduction (third and fourth reduction) also results in continuous changes, such as the disappearance of the two long-wavelength weak absorptions and the red-shift of the two intense bands in the visible range (Figure 6b). Again, we found no evidence for a mixed-valent species [Ni4(L iPr )4] •3− , probably again due to the far higher stability of the [Ni4(L iPr )4] 4− state. For the Zn derivative, the behaviour is very similar (spectra in the supplementary materials).
A detailed look on our spectra reveals that isosbestic points are far from being perfect. This might be due to the fact that more than two species were observed, thus a weak hint for the mixed-valent species. At the same time, the spectra of the highly reduced species show some long-wavelength tailing, indicative of a precipitation of these highly charged complexes. In line with this, the full reversibility of the first four reduction processes through re-oxidation is only obtained upon long standing at 0 V. X-band EPR spectra recorded during cathodic electrolysis of [Ni4(L iPr )4] at potentials between −1.5 and −1.9 V (first and second reduction) gave an isotropic signal at g = 2.016 with only 45 G total spectral width ( Figure S40). This is in line with a pyrazine-centred radical complex, and no indication for a second reduced species was found.

Quantumchemical Calculations
DFT calculations were initiated to obtain insight on the energy and location of the highest occupied molecular orbital (HOMO) and the lowest unoccupied molecular orbital (LUMO) of the complexes. First, we calculated the molecular structures of the complexes in the gas phase on (RI-)BP86/def-SV(P) level and refined some of the resulting structures on (RI-)BP86/def2-TZVP level. Frequency calculations were carried out in order to check for the absence of imaginary frequencies, which confirms an energetic minimum for the geometry. The calculated minimum geometries agree quite well with the experimental structures from HR-MS in solution and with the preliminary single crystal XRD results (Tables S5 and S6).
The calculated LUMOs (Figures 7a and 8a) are largely centred on the pyrazine units in line with the EPR results but also the CNNCN chain of the thiosemicarbazonate contributes markedly, in line with the dependency of the reduction potentials on the R substituents. Importantly, there was no indication of an electronic coupling of the four pyrazine units, in line with the assumed electrostatic nature of the observed splitting of the four reduction waves.   The largest contributions to the calculated HOMOs come from the thiolate S atoms. However, while both S atoms contribute in the same way for the Zn(II) complex (Figure 7b), for the Ni(II) system d orbital contributions were visible in the electron density plots (Figure 8b) seemingly replacing one of the S atom contributions. This is in line with a d orbital control of the coordination in the case of d 8 Ni(II) in contrast to the rather spherical coordination of d 10 Zn(II) [73]. The nature of the calculated HOMOs is also in agreement with the assignment of the observed oxidation waves.

Methods and Instrumentation
Elemental analysis was obtained using a HEKAtech CHNS EuroEA 3000 Analyzer (HEKAtech GmbH, Wegberg, Germany). The NMR spectra were recorded on a Bruker Avance II 300 MHz (Bruker, Rheinfelden, Germany) ( 1 H: 300.13 MHz, 13 C: 75.47 MHz)-double resonance (BBFO) 5 mm observe probehead with a z-gradient coil or on a Bruker Avance II 600 MHz spectrometer (Bruker, Rheinfelden, Germany) ( 1 H: 600.13 MHz, 13 C: 150.93 MHz, 15 N: 60.83 MHz)-triple resonance (TBI) 5 mm inverse probehead with a z-gradient coil. The unambiguous assignment of the 1 H, 13 C, and 15 N resonances was obtained from 1 H COSY, 1 H NOESY, gradient selected 1 H, 13 C HSQC, and HMBC, and gradient selected 1 H and 15 N HMBC experiments. All 2D NMR experiments were performed using standard pulse sequences from the Bruker pulse program library. Chemical shifts were relative to TMS for 1 H and 13 C and to CH3NO2 for 15 N. The spectra analyses were performed by the Bruker TopSpin 3.2 software. UV-Vis absorption spectra were measured on Varian Cary50 Scan or Shimadzu UV-3600 photo spectrometers (Shimadzu Deutschland, Duisburg, Germany). Electrochemical experiments were carried out in 0.1 M THF solutions using a three-electrode configuration (glassy carbon working electrode, Pt counter electrode, and Ag/AgCl pseudo reference) and an Autolab PGSTAT30 potentiostat (Metrohm, Filderstadt, Germany) and function generator. The ferrocene/ferrocenium couple (FeCp2/FeCp2 + ) served as an internal reference. UV-Vis spectroelectrochemical measurements were performed with an optically transparent thin-layer electrochemical (OTTLE) cell [42]. EI-MS spectra were measured with a Finnigan MAT 900 S (MasCom, Bremen, Germany). ESI-MS spectra were recorded on a Bruker Daltonics Esquire 3000 plus (Bruker, Rheinfelden, Germany) and HRMS-ESI spectra were obtained on a Thermo Scientific LTQ Orbitrap XL and LTQ Orbitrap hybrid instrument (Thermo Fisher Scientific Waltham, MA, USA). Simulations were performed using ISOPRO 3.0 programme (M. Senko, download from The largest contributions to the calculated HOMOs come from the thiolate S atoms. However, while both S atoms contribute in the same way for the Zn(II) complex (Figure 7b), for the Ni(II) system d orbital contributions were visible in the electron density plots (Figure 8b) seemingly replacing one of the S atom contributions. This is in line with a d orbital control of the coordination in the case of d 8 Ni(II) in contrast to the rather spherical coordination of d 10 Zn(II) [73]. The nature of the calculated HOMOs is also in agreement with the assignment of the observed oxidation waves.

Methods and Instrumentation
Elemental analysis was obtained using a HEKAtech CHNS EuroEA 3000 Analyzer (HEKAtech GmbH, Wegberg, Germany  [74] or SIR 92 [75], and refinement was carried out with SHELXL 2013 [76] or SHELXL 97 [77] employing full-matrix least-squares methods on F 2 with F 0 2 ≥ 2σ(F 0 2 ). The numerical absorption corrections using X-RED V1.22 (Stoe & Cie, 2001) [78,79] were performed after optimising the crystal shapes using X-SHAPE V1.06 (Stoe & Cie, 1999) [78,79]. Non-hydrogen atoms were refined with anisotropic displacement parameters. H atoms were included using appropriate riding models. The refined tetranuclear structures contained additional disordered solvate molecules. Because no reasonable split model was found, the data were corrected for disordered solvent using the SQUEEZE option in PLATON [80,81]. The R int and the refined R 1 and wR 2 values were generally poor and A level alerts were found in Platon checkcif. Since this includes even data obtained at 170(2) K, we can ascribe this to the generally very low quality of the crystals. We assume that they rapidly decompose upon evaporation of the solvent molecules in the voids (see elemental analyses and thermogravimetric analyses). Data were collected at 100 K. The wavelength was tuned to 0.7205 Å and a Pilatus 2M detector was employed. Data were collected in two wedges of 120 degrees each with Chi angles on the PRIGo goniostat (PSI) [82] set to 0 and 30 degrees, respectively. Data were processed with XDS and XSCALE [83]. Computational details: Ground state electronic structure calculations on complexes were performed on the basis of density-functional theory (DFT) methods with resolution of identity coulomb approximation [84] using the TURBOMOLE 6.6 and 7.0 [85] program packages and the TMoleX 4.1 [86] user interface. For all atoms, the double-ξ-valence def-SVP [87] and the triple-ξ-valence def2-TZVP [88] basis sets were used with Becke's gradient-corrected exchange-energy functional BP86 [89].

General
General information on materials and details on the synthesis of the pyrazine-2,5-carbaldehydes and the bis(thiosemicarbazones) H 2 L R (R = Me, Et, i Pr, Ph) are provided in the Supplementary Materials.

Synthesis of the tetranuclear complexes [M 4 (L R ) 4 ]
General description: To the protoligands H 2 L R dissolved in DMA 0.01 M (1 equiv), we added a 0.044 M solution of KOH (0.2 equiv) in EtOH. This solution was layered carefully using 1 equiv of a 0.01 M solution of the corresponding metal salt (Ni(BF 4 ) 2 or [Zn(acac) 2 ] in MeCN. The reaction mixtures were left to crystallise taking one to two weeks. The materials were filtered off and washed with water and dried at 70 • C or under vacuum. Despite this drying procedure, we found varying amounts of DMA and/or water in the elemental analyses, thermogravimetric analyses, and NMR spectra. was concluded for the pronounced splitting of the reduction waves (up to 300 mV). EPR and UV-Vis spectroelectrochemical measurements fail to detect specific spectra of the mixed-valent species [M 4 (L R ) 4 ] •− and [M 4 (L R ) 4 ] •3− indicative of relatively weak (largely electrostatic) coupling. DFT calculations support these assignments.
In future work, we will study the properties of these new materials in more detail. Especially, we will try to obtain better crystal and molecular structures from single-crystal XRD and explore potential applications, e.g., in multi-electron catalysis. Furthermore, we will expand the coordination chemistry of these new and easy-to-make 1,4-pyrazine-2,5-bis(thiosemicarbazone) ligands. Especially, the electronic connection of various low-valent metals through the 1,4-pyrazine unit might yield materials with interesting optoelectronic properties such as intense absorptions in the visible and NIR for light harvesting. Additionally, the cooperative effects of two connected metals in catalytic applications will be studied.