Biomolecules Electrochemical Sensing Properties of a PMo 11 V @ N-Doped Few Layer Graphene Nanocomposite

A novel hybrid nanocomposite, PMo11V@N-doped few layer graphene, was prepared by a one-step protocol through direct immobilization of the tetrabutylammonium salt of a vanadium-substituted phosphomolybdate (PMo11V) onto N-doped few layer graphene (N-FLG). The nanocomposite characterization by FTIR and XPS confirmed its successful synthesis. Glassy carbon modified electrodes with PMo11V and PMo11V@N-FLG showed cyclic voltammograms consistent with surface-confined redox processes attributed to Mo-centred reductions (MoVI→MoV) and a vanadium reduction (VV→VIV). Furthermore, PMo11V@N-FLG modified electrodes showed good stability and well-resolved redox peaks with high current intensities. The observed enhancement of PMo11V electrochemical properties is a consequence of a strong electronic communication between the POM and the N-doped few layer graphene. Additionally, the electro-catalytic and sensing properties OPEN ACCESS Inorganics 2015, 3 179 towards acetaminophen (AC) and theophylline (TP) were evaluated by voltammetric techniques using a glassy carbon electrode modified with PMo11V@N-FLG. Under the conditions used, the square wave voltammetric peak current increased linearly with AC concentration in the presence of TP, but showing two linear ranges: 1.2 × 10−6 to 1.2 × 10−4 and 1.2 × 10−4 to 4.8 × 10−4 mol dm−3, with different AC sensitivity values, 0.022 A/mol dm−3 and 0.035 A/mol dm−3, respectively (detection limit, DL = 7.5 × 10−7 mol dm−3).


Introduction
Taking advantage of the electroactivity of some drugs and biomolecules, the application of electrochemical sensors for biological analysis has been growing rapidly, mainly due to the simplicity, accuracy, precision, low cost and rapidity of the electrochemical techniques [1].In order to develop electrochemical sensors with higher selectivity and sensitivity, the chemical modification of electrode surfaces has been a major focus of research.The modified electrodes present lower overpotential values and improved mass transfer kinetics, decreasing the effect of interferences and avoiding surface fouling [2].Nanostructured materials, in particular, carbon-based nanomaterials such as carbon nanotubes and graphene, have attracted considerable interest in this field, owing to their unique physical, chemical and electrochemical properties.They present low residual current, readily renewable surfaces and wide potential windows, providing an important and feasible platform for electroanalysis [3,4].
Graphene (G), in particular, emerged as a "superstar" material in the last years, being characterized by a two-dimensional (2D), single-layer sheet of sp 2 -hybridized carbon atoms that are closely packed into a hexagonal lattice structure [5].Its properties, such as fast electron transportation, high thermal conductivity, excellent mechanical strength and high surface area, suggest its ability to detect analyte molecules and to promote a fast electron transfer between the electrode and the analyte, which make it a promising electrocatalyst [3,5].In fact, several reports published show the good sensitivity and electrocatalytic activity of pristine graphene and graphene-based nanocomposites on the electrochemical sensing of biomolecules such as dopamine (DA) [6,7], uric (UA) and ascorbic (AA) acids [8], nucleic acids [9] and glucose [10].
More recently, advances in graphene research showed that the chemical doping with heteroatoms can be an effective strategy to modulate their electronic properties and surface chemistry.Among the several potential dopants, nitrogen is an excellent candidate due to its comparable size with carbon and five valence-electronic structure, which result in strong covalent bonds between the nitrogen and carbon atoms [11].The resulting material-nitrogen-doped graphene (N-G)-presents higher electrical conductivity, much larger functional surface area, more biocompatibility and more chemically active sites for functionalization than pristine G [12], whereby it has been applied in N-G-based electrochemical sensors with enhanced performances to the detection of DA, AA and UA [13,14], glucose [15], H2O2 [16], antibiotics [17] and pesticides [12].
However, the properties of G-type materials may be yet more prized by the incorporation of these materials in nanocomposites, in order to couple their unique properties with interesting properties of other materials [4,10].In this context, polyoxometalates (POMs), a kind of transition metal oxide nanoclusters [18], can be a good option owing to their rich chemical and structural variety and, mainly, due to their electronic properties.POMs have the ability to undergo fast, reversible and stepwise multi-electron reactions without decomposition, making them promising electro-catalysts [19].The incorporation of POMs in nanocomposites with different materials has been reported [20,21].Several POMs@carbon nanotubes (CNTs) composites have been prepared and applied in batteries [22], supercapacitors [23] and for the reduction of chemical species [24], while POM composites with metal nanoparticles, ionic liquids and conducting polymers have been applied for the reduction of H2O2, iodate, nitrite [18,25] and bromate [26], as well as in the sensing of small biomolecules (DA and AA) [19,27].Previously, we also reported the preparation of several POM@MOFs nanocomposites and their application as electro-catalysts towards nitrite and iodate reductions and AA and DA oxidations [28,29].
Acetaminophen (AC), also known as paracetamol, is an analgesic and antipyretic drug widely used for the relief of mild to moderate pain associated with headache, backache, arthritis and postoperative pain.However, overdoses can lead to the accumulation of toxic metabolites, causing severe and sometimes fatal hepatotoxicity and nephrotoxicity [30].Theophylline (TP) is a methyl-xanthine derivative which exists widely in nature and is one of the most commonly used medications for the treatment of the symptoms of chronic asthma [3].Several techniques, including spectrophotometry and liquid chromatography (LC), have been applied for the determination of AC in pharmaceutical formulations and biological fluids, but these methods are time-consuming and expensive.Due to the advantages of low cost, fast response, simple instrumentation and high sensitivity, voltammetric methods are therefore a better solution for the determination of AC in the presence of TP.
This work reports on the preparation of a new nanocomposite, PMo11V@N-FLG, by the immobilisation of the tetrabutylammonium salt of vanadium-substituted phosphomolybdate [PMo11VO40] −5 (PMo11V) into nitrogen-doped few-layer graphene (N-FLG).Investigations were focused on the nanocomposite characterization and its electrochemical properties upon immobilization on a GC electrode surface.The electro-catalytic properties towards AC and TP oxidation were evaluated.In addition, detection of AC in the presence of an excess of TP was also determined.

Nanocomposite Preparation and Characterization
The tetra-butylammonium salt of PMo11V was prepared according to the literature and characterized by several techniques [31].The results obtained are in good agreement with the literature.Figure 1 shows the FTIR spectra of N-FLG, TBA-PMo11V and PMo11V@N-FLG.The FTIR spectrum of TBA-PMo11V shows the vibration bands due to the C-H stretching vibration, at 2871 and 2960 cm −1 , and those due to C-H bending vibration at 1479 and 1374 cm −1 which are characteristic of the TBA salt, two bands in the interval 1080-1040 cm −1 , assigned to the splitting of the P-O stretching vibration, a band at 945 cm −1 assigned to the Mo-Od vibration and the bands at 872 cm −1 and 799 cm −1 to the Mo-Ob-Mo and Mo-Oc-Mo stretching modes, respectively [31][32][33].The FTIR spectrum of N-FLG (Figures 1 (black) and S1) presents very weak and poorly resolved vibrational bands: it shows a broad absorption at around 3455 cm −1 assigned to the stretching vibrations of the OH groups which may be due to hydroxyl groups and residual adsorbed water, and the vibration band at 1639 cm −1 may be due to C=N stretching vibrations [34].The two bands at 2855 and 2926 cm −1 correspond to the aromatic sp 2 C-H stretching vibration.The sharp band at 1383 cm −1 is attributed to C-N stretching vibration [35].The band at 1533 cm −1 is assigned to C=C stretching vibrations of the aromatic carbon, and the band at 1119 cm −1 can be assigned to stretching vibration of C-O [36,37].
The preparation of the nanocomposite PMo11V@N-FLG was carried out by one-step protocol through the direct immobilization of the prepared TBA-PMo11V onto N-FLG.Accordingly, POM immobilization is based in hydrophobic interactions between the alkyl chains of the TBA cation in the sample with the N-FLG surface, which are also hydrophobic: the POM is linked electrostatically to the TBA cations and consequently adsorbed on the N-FLG surface.However, some interactions between Mo and/or V with the N groups present in the N-FLG cannot be excluded.
FTIR spectrum of the nanocomposite confirms the success of the POM immobilization since it exhibits the bands due to N-FLG in the range 1161 to 1631 cm −1 , as well as the vibrational bands due to POM in the range 798-1081 cm −1 and TBA at 2960, 2871, 1479 and 1374 cm −1 .
The deconvoluted high resolution XPS spectra of N-FLG and PMo11V@N-FLG are shown in Figures S2 and S3 as well as the obtained binding energies (Table S1); the surface atomic percentages of each component in both materials are summarized in Table 1.The presence in the nanocomposite of the target elements of each component, N-FLG (C, O, N) and POM (P, Mo, O, N and V) also confirms the successful fabrication of the nanocomposite.The atomic ratios of 1:11 and 1:1 for V:Mo and P:V, respectively, suggest that the POM structure is maintained in the nanocomposite.Furthermore, comparison between the XPS results obtained here with those obtained for the same POM immobilized onto commercial pristine graphene flakes (PMo11V@GF) [38] shows that a higher amount of POM was immobilized onto N-FLG which is one advantage for applications in electrochemical sensing.The C 1s high-resolution spectrum of pristine N-FLG (Figure S2a) was fitted with seven peaks: a main peak at 285.0 eV assigned to the graphitic structure (sp 2 ), a peak at 286.2 eV attributed to C-C and C-N, a peak at 287.2 eV assigned to C-O, a peak at 288.5 eV related to C=O, a peak at 289.9 eV due to -COO and the peaks at 291.2 and 292.4 eV are attributed to the characteristic shake-up satellite for the π-π * transition and to its extension, respectively [39,40].For PMo11V@N-FLG, the C 1s high-resolution spectrum (Figure S3a, Table S1) was also fitted with seven peaks: 285.0, 285.8, 286.6, 287.2, 288.2, 289.3 and 291.6 eV with the same assignment as for N-FLG.The slight differences in the peak positions may be due to some structure rearrangements.
The O 1s high resolution spectrum of N-FLG was fitted with two peaks at 531.3 and 533.0 eV ascribed to O=C from ketone, quinone moieties and to O-C from ether and phenol groups, respectively.The PMo11V@N-FLG O 1s high resolution spectrum was deconvoluted into three peaks with binding energies at 530.9, 532.1 and 533.6 eV.The last two are assigned as described for N-FLG and the first is ascribed to O-Mo [38].
The N 1s high-resolution spectrum of pristine N-FLG was deconvoluted into four peaks: the peak at 399.1 eV is ascribed to pyridinic-N, the peak at 401.6 eV is attributed to pyrrolic-N, the peak at 404.5 eV is assigned to graphitic N and finally that at 407.3 eV may be due to some oxidized N [39,41,42].The presence of N on PMo11V@N-FLG and its atomic percentage could be assessed, but with low certainty due to the overlap of the N 1s and Mo 3p peaks in the N 1s high resolution spectrum.Nevertheless, the spectrum was fitted taking into consideration the existence of the Mo 3p (3p1/2 and 3p3/2) and N 1s peaks.The area obtained through modulation of Mo 3p is similar to that obtained for Mo 3d, suggesting a correct fitting of peaks taking into consideration the N 1s peaks.The two peaks in the N 1s spectrum at 400.9 and 402.6 eV assigned to pyridinic and pyrrolic-N, respectively.However, two other very low intense peaks could be observed at 404.8 and 406.5 eV, but its low intensity prevent its inclusion in the fitting.
The Mo 3d high resolution spectrum was deconvoluted taking into account the 3d5/2 and 3d3/2 doublets caused by spin-orbital coupling.The Mo 3d high resolution spectrum of PMo11V@N-FLG was fitted with two couples of peaks with binding energies, respectively, at 231.8 and 234.9 eV assigned to Mo 5+ and at 233.1 and 236.2 eV assigned to Mo 6+ .The presence of the Mo 5+ species suggests that partial photoreduction of POMs occurred when the sample was exposed to the X-ray source or during the nanocomposites preparation.a Determined by the areas of the respective bands in the high-resolution XPS spectra.

Electrochemical Behaviour
The electroactive surface area of bare GCE, N-FLG/GCE and PMo11V@N-FLG/GCE were determined from cyclic voltammograms of 1 × 10 −3 mol dm −3 K3[Fe(CN)6] in KCl 1 mol dm −3 (Figure S4) using the Randles-Sevcik equation and assuming that electrode processes are controlled by diffusion: (1) where n is the number of electrons involved in the process (1 in this case), A is the electrode surface area (cm 2 ), Dr the diffusion coefficient (cm 2 s −1 ), R the concentration of the species (mol cm −3 ), ν is the scan rate (V s −1 ) and ipa the intensity of the anodic peak current (A) [43].The A values obtained were: 0.0443, 0.0374 and 0.0754 cm 2 for bare GCE, N-FLG/GCE and PMo11V@N-FLG/GCE, respectively.These results indicate that the modification of GCE with N-FLG leads to a small decrease of the electroactive area (≈15%) while GCE modification with PMo11V@N-FLG/GCE changes dramatically the electroactive area with an increase close to 70%, indicating that the introduction of the PMo11V onto N-FLG provides a more conductive pathway for the electron-transfer of [Fe(CN)6] 3−/4− .In addition, the anodic/cathodic peak-to-peak separations (ΔEp) varies between 0.072 and 0.094 V for GCE, 0.378 and 0.781 V for N-FLG/GCE and 0.076 and 0.086 V for PMo11V@N-FLG/GCE for the lowest (0.020 V s −1 ) and highest (0.50 V s −1 ) scan rates used.
Figure 2b depicts the cyclic voltammograms obtained with a PMo11V@N-FLG modified electrode in pH 2.5 H2SO4/Na2SO4 buffer solution and in the potential range +0.90 to −0.50 V.The nanocomposite also shows five redox processes: EpcV1 = 0.317 V, EpcMo1 = 0.079 V, EpcMo2 = −0.032V, EpcMo3 = −0.227V and EpcMo4 = −0.370V vs. Ag/AgCl, corresponding to one-electron oxidation process of vanadium and four two-electron redox processes assignable to the molybdenum centres.All the peaks are much better resolved and have higher current intensities (≈10 times higher) compared with the POM-modified electrode, suggesting faster electron-transfer kinetics, which is associated with the exceptional electronic properties of N-FLG.
In the experimental timescale employed (scan rates in the range 0.02-0.5 V s −1 ) both cathodic (Epc) and anodic (Epa) peak potentials varied less than 0.011 V for PMo11V and 0.010 V for PMo11V@N-FLG.Figure 3 depicts the plots of log ip versus log v for the Mo1 and Mo3 waves of PMo11V and PMo11V@N-FLG modified electrodes.Both ipc and ipa are directly proportional to the scan rate for all peaks, with r 2 between 0.998 and 0.991 for PMo11V and 0.998 and 0.992 for PMo11V@N-FLG, which indicate surface-confined processes [38].In addition, the anodic to cathodic peak-to-peak separations (ΔEp) of all redox couples vary between 0.052 (0.02 V s −1 ) and 0.019 V (0.5 V s −1 ) and the ratios ipa/ipc are close to one (0.96 ± 0.05).Electrochemical surface coverages of modified electrodes with PMo11V and PMo11V@N-FLG were calculated from cyclic voltammetry using the Mo1 reduction process according to the equation: where ip is the peak current (A), n is the number of electrons transferred (2 in this case), ν is the scan rate (V s −1 ), A is the geometric area of the electrode (0.07065 cm 2 ), R is the gas constant, T is the temperature (298 K) and F is Faraday's constant.Peak currents (Mo1) were plotted against scan rate (0.02 to 0.5 V s −1 ) and the value of ip/v obtained was used to calculate the surface coverage.This led to Γ = 0.006 nmol cm −2 for PMo11V and Γ = 0.077 nmol cm −2 for PMo11V@N-FLG.These results show that electrochemical surface coverage for the nanocomposite modified electrodes is significantly higher (almost 13 times) than that of the POM-modified electrodes.This constitutes an outstanding advantage when developing modified electrodes with carbon nanomaterials for applications in electrocatalysis and electroanalytical determinations.The N-FLG allowed the immobilization of a much larger quantity of the electroactive PMo11V on the electrode surface due to its high surface area, and simultaneously improved their electrochemical responses.

Electro-Catalytic Performance of Nanocomposite Modified Electrodes
The electrochemical oxidation of acetaminophen (AC) and theophylline (TP) was initially investigated individually by cyclic voltammetry at the three different electrodes.Figure 4 shows the cyclic voltammograms of 5.0 × 10 −4 mol dm −3 solutions of AC and TP at a bare GCE and at N-FLG/GCE and PMo11V@N-FLG/GCE modified electrodes.With a bare GCE electrode and under the experimental conditions used, AC and TP are irreversibly oxidized at ≈0.762 V and at ≈1.352 V vs. Ag/AgCl, respectively.
At a N-FLG modified electrode, the oxidation of TP occurs at almost the same potential as with a bare GCE (1.360 V), and no significant changes are observed in the peak current, whereas for the PMo11V@N-FLG modified electrode the peak current increases ≈31% when compared with that obtained with a bare GCE (Figure 4a) and the TP cathodic peak shifts 0.055 V to less positive potentials.
For AC, the modification of a GCE with N-FLG also leads to small differences in the peak currents, but Epa shifts to less positive potentials (Epa = 0.676 V).For PMo11V@N-FLG/GCE, the AC oxidation peak is observed at even less positive potentials, c.a. Epa = 0.622 V vs. Ag/AgCl (Epa cathodic shift of ≈0.140 V when compared to the bare GCE) and the peak current increases by ≈57%, similarly to TP.Additionally, a cathodic peak is observed at Epc ≈ 0.559 V which is attributed to the partial reduction of AC, suggesting that the oxidation of AC in the PMo11V@N-FLG modified electrode becomes a quasi-reversible process [36]; the new set of peaks in the potential range 0.45-0.0V corresponds to the PMo11V redox processes.
These results show that the modification of a GCE with PMo11V@N-FLG leads to an improvement in the overall electrochemical performance towards TP and AC oxidations, which is due to the electro-catalytic properties of PMo11V.The electrochemical oxidation of AC and TP was also investigated individually by square wave voltammetry.Figure 5 shows the SWV curves of 5.0 × 10 −4 mol dm −3 solutions of AC and TP at a bare GCE and at N-FLG/GCE and PMo11V@N-FLG/GCE modified electrodes.As observed for cyclic voltammetric results, the immobilization of N-FLG and PMo11V@N-FLG on the GCE surface lead to some changes in the square-wave curves of each species when compared to the GCE.At the bare electrode, the oxidation peaks of AC and TP are observed at 0.725 and 1.309 V vs. Ag/AgCl.
The modification of a GCE with N-FLG leads to small changes in the oxidation potential of TP, but there is a decrease in peak currents of ≈24%.However, for the oxidation of AC there is an increase of 19% in the peak current and the peak is observed at c.a. 0.645 V vs. Ag/AgCl (Epa cathodic shift of ≈0.080 V).These changes suggest that N-FLG has itself a strong interaction with the AC species leading to better detection.Still, much more significant results were obtained for GCE modification with the PMo11V@N-FLG nanocomposite.For the oxidation of TP there is an increase of the peak currents of 47% and a Epa cathodic shift of 0.045 V, while for the AC oxidation the Epa cathodic shift is 0.126 V and there was an increase of 234% in the peak current, suggesting that this modified electrode is more suitable for the determination of AC rather than TP.These effects are crucial for the application of modified electrodes in electro-catalysis.In a PMo11V@N-FLG modified electrode, the high electrical conductivity and large surface area of N-FLG is associated with the unique electro-catalytic properties of PMo11V-its ability to accept and release a high number of electrons without decomposition.Consequently, both components contribute to the overall improvement of the electrocatalytic performance.The sensitivity of the PMo11V@N-FLG modified electrode towards the oxidation of AC and TP was found to be 0.040 and 0.011 A/mol dm −3 , respectively.concentration of AC from 1.2 × 10 −6 to 4.8 × 10 −4 mol dm −3 in the presence of TP: inset in Figure 6.Two different linear regions can be observed: one from 1.2 × 10 −6 to 1.2 × 10 −4 mol dm −3 (i = 0.022 cAC + 0.018, r 2 = 0.998), leading to a detection limit 7.5 × 10 −7 mol dm −3 (detection limit calculated through the equation 3 σ/slope, where σ is the standard deviation of the blank), and a second linear region from 1.2 × 10 −4 to 4.8 × 10 −4 mol dm −3 (i = 0.035 cAC − 1.58, r 2 = 0.995).Taking into account that the 'breaking point' in the plot in the inset of Figure 6 corresponds to an AC concentration of 1.2 × 10 −4 mol dm −3 , which is very similar to the concentration of TP in solution (1.0 × 10 −4 mol dm −3 ), these results may suggest some competition effects between TP and AC: when TP is in larger quantities than AC, a lower AC sensitivity (0.022 A/mol dm −3 ) is observed when compared to the sensitivity value when AC concentration exceeds that of TP (0.035 A/mol dm −3 ).

Determination of AC in the Presence of TP at a PMo11V@N-FLG/GCE
To the best of our knowledge, there are no reports in the literature concerning the use of modified electrodes with a PMo11V@N-FLG nanocomposite for the determination of AC in the presence of TP.Furthermore, the obtained detection limit is better than that (8.1 × 10 −6 mol dm −3 ) reported for a similar study at a ferrocene-derivative modified-graphene paste electrode [3].
Finally, the stability and robustness of the PMo11V@N-FLG/GCE was evaluated by measuring and comparing the ipa of 0.5 mM AC in a pH 2.5 H2SO4/Na2SO4 buffer solution at the modified electrode at the begging and at the end of all the experiments (data not shown).No significant change in peak position was observed and the peak current only decreased by 11%, confirming the electrochemical stability of the PMo11V@N-FLG modified electrode.This loss might be due to the partial depletion of nanocomposite from the electrode surface due to consecutive use.

Materials and Methods
The N-doped few layer graphene (N-FLG) was prepared by fluidized bed chemical vapor deposition process by the decomposition of a mixture of ethylene and ammonia in the presence of a ternary oxide powder catalyst at 650 °C [44].The N-FLG powder was recovered after washing the powder in 35% HCl at 25 °C.The tetra-butylammonium salt of the vanadium-phosphomolybdate, PMo11V was prepared according to the literature and characterized by several techniques [31] and the PMo11V@N-FLG was prepared using an adapted procedure [38].Briefly, an acetonitrile solution (2.5 mL) of the compound (20 mg) was added to a toluene dispersion (25 mL) of the N-FLG (20 mg).The resulting yellowish solutions were vigorously stirred at room temperature for 10 min.The solutions were left to rest for 1 h and became colourless (the absence of POM in solution was confirmed by UV-Vis spectroscopy), suggesting a 100% efficiency POM immobilization onto the surfaces of N-FLG, leading to: 1.82 mmol of POM/g nanocomposite.Then, the toluene was removed and the resulting nanocomposite was dried under vacuum at 40 °C.
The Fourier transform infrared (FTIR) spectra were performed in a Jasco FT/IR-460 Plus spectrophotometer (Jasco, Easton, USA) in the range 400-4000 cm −1 , using a resolution of 4 cm −1 and 32 scans; the spectra were obtained in KBr pellets (Merck, spectroscopic grade) containing 0.2% weight of PMo11V@N-FLG.
The X-ray photoelectron spectroscopy (XPS) measurements were performed at CEMUP (Porto, Portugal), in a VG Scientific ESCALAB 200A spectrometer (VG Scientific, UK) using non-monochromatized Al Kα radiation (1486.6 eV).To correct possible deviations caused by electric charge of the samples, the C 1s band at 285.0 eV was taken as the internal standard.The XPS spectra were deconvoluted with the XPSPEAK 4.1 software (VG Scientific, UK), using non-linear least squares fitting routine after a Shirley-type background subtraction.The surface atomic percentages were calculated from the corresponding peak areas and using the sensitivity factors provided by the manufacturer.

Electrochemical Studies
An Autolab PGSTAT 30 potentiostat/galvanostat controlled by a GPES software (EcoChimie B.V, Utrecht, The Netherlands) was used for cyclic voltammetry (CV) and square-wave voltammetry (SWV).A conventional three-electrode compartment cell was used.The working electrode was a glassy carbon electrode, GCE, (3 mm diameter, BAS, MF-2012), the auxiliary and reference electrodes were a platinum wire (7.5 cm, BAS, MW-1032) and Ag/AgCl (sat.KCl) (BAS, MF-2052), respectively.The cell was enclosed in a grounded Faraday cage and all studies were carried out at room temperature and kept under an argon flow.A combined glass electrode (Crison) connected to a pH meter Basic 20 + (Crison) was used for the pH measurements.
Electrolyte solutions for cyclic voltammetry were prepared using ultra-pure water (resistivity 18.2 MΩ cm at 25 °C, Millipore, Porto, Portugal).The H2SO4/Na2SO4 buffer solution with pH = 2.5 used for electrochemical studies was prepared by mixing appropriate amounts of a 0.2 mol dm −3 H2SO4 solution with a 0.5 mol dm −3 Na2SO4 solution.
The dispersion used to produce the modified electrodes was prepared as follows: a N,N-dimethylformamide (DMF) dispersion (3 mL) of N-FLG or PMo11V@N-FLG (1 mg) was sonicated for 10 min.
Prior to modification, the GCE electrode was conditioned by a polishing/cleaning procedure using diamond pastes of 6, 3 and 1 µm (MetaDi II, Buehler, Düsseldorf, Germany) followed by aluminium oxide of particle size 0.3 µm (Buehler, Düsseldorf, Germany) on a microcloth polishing pad (BAS Bioanalytical Systems Inc., Warsaw, Poland), and then the electrode was rinsed with ultra-pure water and finally sonicated for 5 min in ultra-pure water.Electrode modification consisted in depositing a 3 μL drop of the selected material in dimethylformamide solution onto the surface of the GCE and the solvent was allowed to evaporate for about 30 min at room temperature.

Conclusions
The vanadium-substituted phosphomolybdate PMo11V was successfully immobilized on N-doped few layer graphene.X-ray photoelectron spectroscopy revealed that the POM structure was kept in the PMo11V@N-FLG nanocomposite.The preparation of modified electrodes with PMo11V@N-FLG was easy and quick to perform, leading to reproducible and stable modified electrodes.PMo11V@N-FLG/GCE revealed five well defined, surface-confined redox processes that were attributed to four molybdenum centres (Mo VI →Mo V ) and a vanadium centre (V V →V IV ).The PMo11V@N-FLG nanocomposite immobilization on a GCE allowed a higher PMo11V surface coverage compared to the direct immobilization of PMo11V.This constitutes an outstanding advantage for electrocatalytic and sensing applications: PMo11V@N-FLG showed excellent electrocatalytic properties towards AC and TP oxidations and allowed the detection of AC in the presence of an excess of TP.

Figure 6
Figure6shows selected square wave voltammograms recorded for the addition of increasing concentrations of AC.The oxidation peak current of AC increases linearly with the increasing

Table 1 .
XPS surface atomic percentages for N-FLG and PMo11V@N-FLG a .