TiO 2 -Embedded Biocompatible Hydrogel Production Assisted with Alginate and Polyoxometalate Polyelectrolytes for Photocatalytic Application

: The hybrid hydrogel materials meet important social challenges, including the photocatalytic puriﬁcation of water and bio-medical applications. Here, we demonstrate two scenarios of polyacrylamide-TiO 2 (PAAm@TiO 2 ) composite hydrogel design using calcium alginate (Alg-Ca) or Keplerate-type polyoxometalates (POMs) {Mo 132 } tuning the polymer network structure. Calcium alginate molding allowed us to produce polyacrylamide-based beads with an interpenetrating network ﬁlled with TiO 2 nanoparticles Alg-Ca@PAAm@TiO 2 , demonstrating the photocatalytic activity towards the methyl orange dye bleaching. Contrastingly, in the presence of the POM, the biocompatible PAAm@TiO 2 @Mo 132 composite hydrogel was produced through the photo-polymerization approach (under 365 nm UV light) using vitamin B2 as initiator. For both types of the synthesized hydrogels, the thermodynamic compatibility, swelling and photocatalytic behavior were studied. The inﬂuence of the hydrogel composition on its structure and the mesh size of its network were evaluated using the Flory–Rehner equation. The proposed synthetic strategies for the composite hydrogel production can be easily scaled up to the industrial manufacturing of the photocatalytic hydrogel beads suitable for the water treatment purposes or the biocompatible hydrogel patch for medical application.


Introduction
The hydrogel structure design is one of the key aspects of modern materials engineering [1].In contrast to labor techniques of macromolecules covalent modification, the desired hydrogel properties can be reached through the different approaches, such as inorganicorganic composite production and the interpenetrating polymeric network formation [2].The latter case can also be considered as a composite, but organic-organic, where the difference between the nature of constituent polymers gives a synergetic effect [3].For instance, using the biocompatible non-ionogenic polyacrylamide (PAAm) matrix and polyelectrolyte alginate (Alg) macromolecules, one can combine mechanical properties of PAAm and Alg capability to immediate gel formation when contacted with Ca 2+ cations (Alg-Ca).Such an approach opens the way to control both the gel's shape and the hydrogel penetration capability [4].This is why this system was chosen to produce the TiO 2 -embedded hydrogel beads for photocatalytic application [5].
The photoactive hydrogel production is a promising solution for the water purification process that has a crucial impact on human life across the world [6].In line with the general green chemistry concept, the widespread toxic organic pollutants can be removed by means of cost-effective technologies using the sunlight as renewable and inexpensive energy sources [7,8] to generate the reactive oxygen species (ROS).Titanium dioxide is a well-known semiconductor material that has been widely used for ROS production since 1972 due to the work of Fujishima and Honda, which first demonstrated its photocatalytic activity.Due to the high activity and chemical stability, TiO 2 nanoparticles destroy organic pollutants via the formation of superoxide radicals on the surface [5,9].However, the photocatalytic application of TiO 2 is limited by the aggregation of nanoparticles in aqueous suspensions.In addition, the problem for environmental application is the difficulty of the particle extraction from the aqueous media [10].The encapsulation of the TiO 2 nanoparticles in the hydrogel matrix is a promising approach to overcome these limits [11].Such hydrogel matrixes demonstrate the effective swelling behavior in aqueous media followed with the high adsorption capacity of pollutants: organic dyes, pesticides, and pharmaceuticals.It is especially actual when the natural biodegradable polymers, such as polysaccharides, are used as sorbents [12,13].
Among many polysaccharides, calcium alginate-based hydrogel is the most widely used material [12,14].At the same time, calcium alginate hydrogels have such disadvantageous as low physical and mechanical properties, which, however, can be improved by combining polysaccharide matrixes with the synthetic polymers [4].In Reference [15], interpenetrating hydrogel networks based on the calcium alginate with acrylamide were reported, which were able to withstand compression deformation of more than 90% with complete restoration of the original shape.The authors suggest that the resulting improved mechanical properties of the interpenetrating networks are due to the formation of the secondary cross-links between the acrylamide's amino groups and the alginate's carboxyl groups, as well as the ability of the ionic cross-links between alginate chains to break during the deformation and recover after unloading [15].
In contrast to labor-consuming and expensive methods [16] of PAAm-Alg composite film photo-polymerization, we used alternative approach coming from synthesis of poly-N-isopropylacrylamide hydrogel beads by using the calcium alginate as a polymerization mold [17].We extended this simple hydrogel formation method to production of PAAm-Alg hydrogel with embedded TiO 2 nanoparticles, further denoted as Alg-Ca@PAAm@TiO 2 .
The second scenario we used for composite hydrogel production is important for bio-application.To avoid any hazardous species such as ammonium persulfate or TEMED (N,N,N',N'-tetramethylethane-1,2-diamine), we used riboflavin (vitamin B2) as photoinitiator of radical polymerization under UV (365 nm) irradiation.The key component in such a system is Keplerate-type polyoxomolybdate (POM) {Mo 132 }=(NH 4 ) 42 [Mo VI  72 Mo V 60 O 372 (CH 3 COO) 30 (H 2 O) 72 ]•(ca.300H 2 O)•(ca.10CH 3 COONH 4 ).This POM is readily dissolved in water, forming multicharged (maximal charge 42−) macroanions with a diameter of 2.9 nm [18].Taking into account the large POM's surface area (26.4 nm 2 ) with many negatively charged centers, the {Mo 132 } can be considered as the inorganic polyelectrolyte unit.Being previously associated with cationic drugs, Keplerate {Mo 132 } can be embedded into PAAm hydrogel structures to provide prolonged drug release [19,20].Moreover, if rhodamine-B is covalently bounded to the polymeric matrix, the pH-depended Keplerate destruction will lead to arising the quenched fluorescence signal [19,21].In the presence of TiO 2 , the mechanical characteristics of photo-polymerized hydrogel can be improved [22].Therefore, mutual presence of Keplerate {Mo 132 } and the TiO 2 nanoparticles pave the way for multifunctional biocompatible hydrogel production with drug release function.
As was shown previously, {Mo 132 } can trap the free radical [23], leading to a decrease in the polymerization rate.However, here we demonstrated that 15% PAAm hydrogel with the embedded TiO 2 nanoparticles, cross-linked by N,N-methylenbisacrylamide (BIS-AAm), can be produced only in the presence of {Mo 132 } under neutral pH-value.In the literature, there is the protocol for photo-polymerized 16% PAAm hydrogel production in the presence of TiO 2 nanoparticles in the acidic condition [22].The found specific behavior of PAAm@TiO 2 @Mo 132 demonstrates that the use of the Keplerate POM is a powerful approach for hydrogel structure design on both the nanoscale and the macroscopic levels.
In brief, the current work shows that the two different strategies of PAAm-based hydrogel production aimed to control the photocatalytic behavior or bioinspired application by means of Alg-PAAm interpenetrating network formation or insertion of {Mo 132 }, respectively.The produced hydrogels were examined on: photocatalytic degradation of methyl orange dye; swelling/re-swelling and thermodynamic behavior; and influence of components ratio on the hydrogel structure.Finally, the two types of hydrogel materials, Alg-Ca@PAAm@TiO 2 photocatalytic beads and biocompatible hydrogel patches PAAm@TiO 2 @Mo 132 , were produced.

Results and Discussion
2.1.Swelling Behavior and Thermodynamic Study of the Alg-Ca@PAAm@TiO 2 Beads The scheme of the hydrogel preparation is presented in Figure 1.When PAAm content is changed from 50 to 95 wt.%, the swelling ratio of the polymeric network (α') increases for both blank Alg-Ca@PAAm and composite Alg-Ca@PAAm@TiO 2 hydrogels reaching the maximal value at 90-95 wt.% of PAAm, which is almost twice as high as the swelling ratio at the PAAm content 50 wt.%(Figure 2). the protocol for photo-polymerized 16% PAAm hydrogel production in the presence of TiO2 nanoparticles in the acidic condition [22].The found specific behavior of PAAm@TiO2@Mo132 demonstrates that the use of the Keplerate POM is a powerful approach for hydrogel structure design on both the nanoscale and the macroscopic levels.
In brief, the current work shows that the two different strategies of PAAm-based hydrogel production aimed to control the photocatalytic behavior or bioinspired application by means of Alg-PAAm interpenetrating network formation or insertion of {Mo132}, respectively.The produced hydrogels were examined on: photocatalytic degradation of methyl orange dye; swelling/re-swelling and thermodynamic behavior; and influence of components ratio on the hydrogel structure.Finally, the two types of hydrogel materials, Alg-Ca@PAAm@TiO2 photocatalytic beads and biocompatible hydrogel patches PAAm@TiO2@Mo132, were produced.

Swelling Behavior and Thermodynamic Study of the Alg-Ca@PAAm@TiO2 Beads
The scheme of the hydrogel preparation is presented in Figure 1.When PAAm content is changed from 50 to 95 wt.%, the swelling ratio of the polymeric network (α') increases for both blank Alg-Ca@PAAm and composite Alg-Ca@PAAm@TiO2 hydrogels reaching the maximal value at 90-95 wt.% of PAAm, which is almost twice as high as the swelling ratio at the PAAm content 50 wt.%(Figure 2).Whereas the increasing content of PAAm in the interpenetrating networks Alg-Ca@PAAm strongly affects the swelling ratio, the addition of the TiO2 nanoparticles has almost no effect on its value.Implicitly, it points out to the absence of strong interaction between the polymeric matrix and titanium dioxide.Indeed, the positive enthalpy of PAAm adhesion to the TiO2 has been shown recently [24].In order to evaluate the thermodynamics of the interaction in Alg-Ca@PAAm@TiO2, we examined the enthalpy of alginate adhesion to the TiO2 nanoparticles.
The enthalpy of an interaction among the polymer and the solid TiO2 nanoparticles was determined for the model of binary composites in which the TiO2 nanoparticles were embedded into the polymer matrix comprised of the linear polymeric chains.The crosslinked polymeric matrix cannot be used in this approach.Consequently, in the microcalorimetry study, the linear sodium alginate (Alg-Na) was used for the composite preparation.Swelling ratio (α') of Alg-Ca@PAAm blank hydrogel hydrogels and Alg-Ca@PAAm@TiO2 composite hydrogel with various polyacrylamide content in polymer matrix.
To evaluate the enthalpy of an interaction among the polymer and solid filler, one should elaborate an appropriate thermochemical (Hess) cycle, which includes measurable processes containing the desired process as their combination [25].In the case of the interaction enthalpy in the polymer/solid composite (∆Hint), such a thermochemical cycle consists of the following steps: (1) wetting of the air-dry solid TiO2 particles in liquid (∆H1); (2) dissolving of the polymer in the same solvent (∆H2); (3) mixing of the solid suspension Swelling ratio (α') of Alg-Ca@PAAm blank hydrogel hydrogels and Alg-Ca@PAAm@TiO 2 composite hydrogel with various polyacrylamide content in polymer matrix.
Whereas the increasing content of PAAm in the interpenetrating networks Alg-Ca@PAAm strongly affects the swelling ratio, the addition of the TiO 2 nanoparticles has almost no effect on its value.Implicitly, it points out to the absence of strong interaction between the polymeric matrix and titanium dioxide.Indeed, the positive enthalpy of PAAm adhesion to the TiO 2 has been shown recently [24].In order to evaluate the thermodynamics of the interaction in Alg-Ca@PAAm@TiO 2 , we examined the enthalpy of alginate adhesion to the TiO 2 nanoparticles.
The enthalpy of an interaction among the polymer and the solid TiO 2 nanoparticles was determined for the model of binary composites in which the TiO 2 nanoparticles were embedded into the polymer matrix comprised of the linear polymeric chains.The cross-linked polymeric matrix cannot be used in this approach.Consequently, in the microcalorimetry study, the linear sodium alginate (Alg-Na) was used for the composite preparation.
To evaluate the enthalpy of an interaction among the polymer and solid filler, one should elaborate an appropriate thermochemical (Hess) cycle, which includes measurable processes containing the desired process as their combination [25].In the case of the interaction enthalpy in the polymer/solid composite (∆H int ), such a thermochemical cycle consists of the following steps: (1) wetting of the air-dry solid TiO 2 particles in liquid (∆H 1 ); (2) dissolving of the polymer in the same solvent (∆H 2 ); (3) mixing of the solid suspension and the polymer solution (∆H 3 ); (4) dissolving of the polymer/solid composites with known TiO 2 : Polymer ratio in the solvent (∆H 4 ).The resulted value for the interaction enthalpy is calculated using the following equation: where ω 1 , ω 2 are the weight fractions of the polymer and TiO 2 nanoparticles in the composite, respectively.Note that the cycle given by Equation (1) includes the dissolution of the polymer and polymeric composites and therefore it cannot be elaborated for the cross-linked polymers because the polymeric composites cannot dissolve but only swell.
As the polymer and the filler are mutually insoluble, upon the composite formation there is only one source to change the enthalpy, which is the interaction at the phase boundary.Therefore, the calculated ∆H int is related to the interaction between the TiO 2 nanoparticles and the polymer chains.In Figure 3a, there is the dependence of the ∆H 3 values on the TiO 2 weight fraction in the polymeric composite.At zero weight concentration of the TiO 2 nanoparticles, the ∆H 3 value corresponds to the dissolution enthalpy of the Alg-Na as an individual polymer (∆H 1 ).The wetting enthalpy of the TiO 2 nanoparticles (∆H 2 ) is given by the value at the right ordinate axis at the TiO 2 weight fraction equals to unity, that is, −5 J•g −1 .With increasing TiO 2 weight fraction in polymeric composite, the dissolution enthalpy decreases in modulo.In accordance with Equation (1), the ∆H int was calculated at a different TiO 2 content, demonstrating the absence of thermodynamic benefit from alginate adhesion onto TiO 2 nanoparticles.
Although the values of the interaction enthalpy given in Figure 3b were obtained for binary linear polymer/solid composites, we can suppose that the major features of the interaction between the polymeric chain and the solid surface of the TiO 2 nanoparticles preserve in the hydrogels as well.Because the interaction between the individual PAAm and the solid surface of the TiO 2 is also energetically unfavorable [26], the polymeric sub-chains of both the PAAm and Alg-Ca networks do not adsorb on the TiO 2 nanoparticles' surface.As a result, the TiO 2 centers remain accessible to participate in the photocatalytic process.In addition to the examination of swelling behavior tightly related to the thermodynamics of "TiO2-Polymeric matrix" interaction, one of the major parameters for the actual application of the Alg-Ca@PAAm@TiO2 beads is their re-swelling because of the logistic needs to transport the hydrogel in a dried state.
The possibility of the Alg-Ca@PAAm@TiO2 hydrogel beads to restore their shape after drying was studied.This property is important for the practical application of photocatalyst hydrogels.In Figure 4, the re-swelling ability of the previously dried Alg-Ca@PAAm@TiO2 hydrogel is shown depending on the PAAm content in the hydrogel interpenetrating networks.Re-swelling ability was calculated through the comparison of the current swelling ratio of the dried hydrogels, with one for the samples swelling immediately after preparation (see Section 3).
In line with the swelling behavior of the Alg-Ca@PAAm@TiO2 hydrogels, the reswelling ability increases with increasing of the PAAm content.However, the full recovery of the swelling ratio value was not observed.For instance, the hydrogel beads sample with low PAAm content reduced its re-swelling ability almost by 80%.The reduction in the swelling ratio of dried sample can be associated with the irreversible collapse of the Alg-Ca matrix, where the polymer chains are attached to each other by multiple hydrogen bonds in addition to Ca 2+ cations [27].
Thus, the covalently (via BIS-AAm) cross-linked PAAm network provides the reswelling ability and prevents the UV-degradation of the Alg-Ca@PAAm@TiO2 composite hydrogel beads.Most likely, the improved physicomechanical properties of the Alg-Ca@PAAm interpenetrating networks are due to the secondary cross-link formation between amino groups of the acrylamide units in PAAm and carboxyl groups in Alg [15].Furthermore, there is a synergetic effect, which stems both from: (i) the steric hindrance of the alginate chains, tangled with PAAm network, preventing the collapse of Alg-Ca mold; (ii) the hydrogel bead formation due to the fast gelation rate caused with the diffusion of Ca 2+ cations from the solution into the Alg-PAAm drop.In addition to the examination of swelling behavior tightly related to the thermodynamics of "TiO 2 -Polymeric matrix" interaction, one of the major parameters for the actual application of the Alg-Ca@PAAm@TiO 2 beads is their re-swelling because of the logistic needs to transport the hydrogel in a dried state.
The possibility of the Alg-Ca@PAAm@TiO 2 hydrogel beads to restore their shape after drying was studied.This property is important for the practical application of photocatalyst hydrogels.In Figure 4, the re-swelling ability of the previously dried Alg-Ca@PAAm@TiO 2 hydrogel is shown depending on the PAAm content in the hydrogel interpenetrating networks.Re-swelling ability was calculated through the comparison of the current swelling ratio of the dried hydrogels, with one for the samples swelling immediately after preparation (see Section 3).Re-swelling ability of the dried Alg-Ca@PAAm@TiO2 hydrogel beads with various PAAm content.The insert presents the re-swelling process ability of the dried Alg-Ca@PAAm@TiO2 hydrogel beads with 50 wt.% of PAAm.

The Photocatalytic Properties of the Alg-Ca@PAAm@TiO2 Hydrogel Beads
In the beginning, we study the photostability of Alg-Ca@TiO2 hydrogel beads and how the PAAm content influences that.In Figure 5, the measurements of UV-degradation degree after 5h irradiation are presented.Re-swelling ability of the dried Alg-Ca@PAAm@TiO 2 hydrogel beads with various PAAm content.The insert presents the re-swelling process ability of the dried Alg-Ca@PAAm@TiO 2 hydrogel beads with 50 wt.% of PAAm.
In line with the swelling behavior of the Alg-Ca@PAAm@TiO 2 hydrogels, the reswelling ability increases with increasing of the PAAm content.However, the full recovery of the swelling ratio value was not observed.For instance, the hydrogel beads sample with low PAAm content reduced its re-swelling ability almost by 80%.The reduction in the swelling ratio of dried sample can be associated with the irreversible collapse of the Alg-Ca matrix, where the polymer chains are attached to each other by multiple hydrogen bonds in addition to Ca 2+ cations [27].
Thus, the covalently (via BIS-AAm) cross-linked PAAm network provides the reswelling ability and prevents the UV-degradation of the Alg-Ca@PAAm@TiO 2 composite hydrogel beads.Most likely, the improved physicomechanical properties of the Alg-Ca@PAAm interpenetrating networks are due to the secondary cross-link formation between amino groups of the acrylamide units in PAAm and carboxyl groups in Alg [15].Furthermore, there is a synergetic effect, which stems both from: (i) the steric hindrance of the alginate chains, tangled with PAAm network, preventing the collapse of Alg-Ca mold; (ii) the hydrogel bead formation due to the fast gelation rate caused with the diffusion of Ca 2+ cations from the solution into the Alg-PAAm drop.

The Photocatalytic Properties of the Alg-Ca@PAAm@TiO 2 Hydrogel Beads
In the beginning, we study the photostability of Alg-Ca@TiO 2 hydrogel beads and how the PAAm content influences that.In Figure 5, the measurements of UV-degradation degree after 5 h irradiation are presented.

Figure 4.
Re-swelling ability of the dried Alg-Ca@PAAm@TiO2 hydrogel beads with various PAAm content.The insert presents the re-swelling process ability of the dried Alg-Ca@PAAm@TiO2 hydrogel beads with 50 wt.% of PAAm.

The Photocatalytic Properties of the Alg-Ca@PAAm@TiO2 Hydrogel Beads
In the beginning, we study the photostability of Alg-Ca@TiO2 hydrogel beads and how the PAAm content influences that.In Figure 5, the measurements of UV-degradation degree after 5h irradiation are presented.The absence of measurable weight loss for blank Alg-Ca hydrogel was found.During the UV irradiation, the Alg-Ca blank hydrogels (without of the TiO2 particles) did not degradate, regardless of initial sodium alginate (Alg-Na) concentration.To the contrary, the Alg-Ca@TiO2 composite hydrogels were partly destroyed (see insert in Figure 5).Weight loss values are plotted as a function of the of the Alg-Na concentration, which progressively decreased with the increase in the sodium alginate content.The substantial The absence of measurable weight loss for blank Alg-Ca hydrogel was found.During the UV irradiation, the Alg-Ca blank hydrogels (without of the TiO 2 particles) did not degradate, regardless of initial sodium alginate (Alg-Na) concentration.To the contrary, the Alg-Ca@TiO 2 composite hydrogels were partly destroyed (see insert in Figure 5).Weight loss values are plotted as a function of the of the Alg-Na concentration, which progressively decreased with the increase in the sodium alginate content.The substantial weight loss values equal to 32 and 23% were found for hydrogel with 0.5 and 1.5% Alg-Na concentration, respectively.The higher the Alg-Na concentration, the better the UVstability of the hydrogel.However, even moderate decomposition of the Alg-Ca@TiO 2 hydrogels under UV irradiation is not appropriate for practical application.Moreover, the measurements of the photocatalytic activity of such gels are incorrect-there is a high probability of the individual TiO 2 particles present in solution, which significantly distort the photocatalytic measurements results.
In contrast, during the 5 h under UV irradiation, the weight loss of Alg-Ca@PAAm@TiO 2 hydrogels was not observed (Figure S5, see Supporting Information).Therefore, this type of hydrogel matrix, which is more promising for the photocatalytic application, was examined towards the bleaching of methyl orange dye in the aqueous solution under UV irradiation.Furthermore, the IR spectra of swelled Alg-Ca@PAAm@TiO 2 and Alg-Ca@PAAm hydrogels (with PAAm 90% of content) after one-year storage in distilled water were measured (Figure S6, see Supporting Information).These spectra demonstrate both the stability of the hydrogel structure and that the addition of TiO 2 does not change the hydrogel structure significantly, which is in line with the thermodynamic data we obtained.
In order to uncover how the presence of the PAAm in the interpenetrating polymer networks affect the photocatalytic activity, we measured the residual concentration of the methyl orange dye in solution exposed with Alg-Ca@PAAm@TiO 2 hydrogel beads containing the different PAAm content (Figure 6a).In line with the first-order reaction kinetics model, the rate constant of methyl orange bleaching can be expressed by this equation [28]: where C 0 is the initial dye concentration; C is the residual dye concentration; τ is the duration of UV irradiation; k is an effective kinetic constant.
Na concentration, respectively.The higher the Alg-Na concentration, the better the UVstability of the hydrogel.However, even moderate decomposition of the Alg-Ca@TiO2 hydrogels under UV irradiation is not appropriate for practical application.Moreover, the measurements of the photocatalytic activity of such gels are incorrect-there is a high probability of the individual TiO2 particles present in solution, which significantly distort the photocatalytic measurements results.In contrast, during the 5 h under UV irradiation, the weight loss of Alg-Ca@PAAm@TiO2 hydrogels was not observed (Figure S5, see Supporting Information).Therefore, this type of hydrogel matrix, which is more promising for the photocatalytic application, was examined towards the bleaching of methyl orange dye in the aqueous solution under UV irradiation.Furthermore, the IR spectra of swelled Alg-Ca@PAAm@TiO2 and Alg-Ca@PAAm hydrogels (with PAAm 90% of content) after oneyear storage in distilled water were measured (Figure S6, see Supporting Information).These spectra demonstrate both the stability of the hydrogel structure and that the addition of TiO2 does not change the hydrogel structure significantly, which is in line with the thermodynamic data we obtained.
In order to uncover how the presence of the PAAm in the interpenetrating polymer networks affect the photocatalytic activity, we measured the residual concentration of the methyl orange dye in solution exposed with Alg-Ca@PAAm@TiO2 hydrogel beads containing the different PAAm content (Figure 6a).In line with the first-order reaction kinetics model, the rate constant of methyl orange bleaching can be expressed by this equation [28]: where C0 is the initial dye concentration; C is the residual dye concentration; τ is the duration of UV irradiation; k is an effective kinetic constant.As one can see, the most rapid decrease in the dye concentration was observed in the case of the Alg-Ca@PAAm@TiO2 hydrogel beads with 90 wt.%PAAm.In the case of the hydrogel sample with 50 wt.%PAAm content, the photo-bleaching degree of the dye solution was much slower.The linear regressions by the first-order kinetics perfectly fit the As one can see, the most rapid decrease in the dye concentration was observed in the case of the Alg-Ca@PAAm@TiO 2 hydrogel beads with 90 wt.%PAAm.In the case of the hydrogel sample with 50 wt.%PAAm content, the photo-bleaching degree of the dye solution was much slower.The linear regressions by the first-order kinetics perfectly fit the experimental data, and the slope gives the value for the effective kinetic constant.For the Alg-Ca@PAAm@TiO 2 hydrogels with PAAm contents of 50, 70, 80 and 90 wt.%, the found values of k are: 0.2 × 10 −3 , 0.7 × 10 −3 , 1.5 × 10 −3 and 2.6 × 10 −3 min −1 , respectively.Among all studied Alg-Ca@PAAm@TiO 2 hydrogels, the sample with PAAm 90 wt.% demonstrated the significantly higher swelling ratio (α' = 20), whereas the others, with 50, 70 and 80 wt.% of PAAm, possessed lower swelling ratio values: 13, 14.5 and 14, respectively (Figure 2).Consequently, the found differences in the photocatalytic behavior (Figure 6a) are not only due to the different swelling behavior.However, in general, the increasing of PAAm content in Alg-Ca@PAAm@TiO 2 leads to the exponential-like growth of the effective kinetic constant (Figure 6b).For ROS species produced on the TiO 2 nanoparticle's surface, the diffusion coefficients are 1-3 orders smaller than those for molecules with a similar size [29].Therefore, the photocatalytic destruction of methyl orange dye should occur near the TiO 2 surface.This process is mainly controlled by diffusion of the dye molecules from outer solution into the hydrogel and back removing of the dye's destruction product.Actually, the accessibility of photocatalyst surface is also strictly important, and in this respect, the low affinity of the alginate or PAAm polymeric chains to the TiO 2 nanoparticles is an advantage [24].Consequently, the effective kinetic constant of methyl orange bleaching should strongly depend on the Alg-Ca@PAAm@TiO 2 hydrogel swelling ratio (Figure 6b).Interestingly, within the PAAm content range of 50-80 wt.%, the swelling ratio is kept on the same level of 13-14.5, while the effective kinetic constant grows exponential in this series.Thus, we can suppose that some intrinsic parameter of such hydrogel has an impact on the photocatalytic activity.One of the possible parameters governing the accessibility of photocatalyst surface is the disaggregation of TiO 2 nanoparticles providing homogeneous distribution across the whole hydrogel structure.Indeed, the increasing of PAAm content leads to more effective disconnection of alginate chains in the Alg-Ca network because when collapsed the Alg-Ca mold can encapsulate the TiO 2 nanoparticles, making them isolated and non-accessible.

Swelling Behavior of the PAAm@Mo 132 @TiO 2 Hydrogel
The macroscopic hydrogel behavior is very sensitive to molecular structure.For instance, the increasing of the PAAm content just by 10 wt.% in Alg-Ca@PAAm@TiO 2 hydrogel leads to the effective kinetic constant of photobleaching is two times increased.Following the conception of the rational design of the hydrogel structure, we studied how the PAAm:BIS-AAm ratio, duration of an exposition under the UV light and the presence of the nanoscaled template {Mo 132 } affect the mesh size of the hydrogel network (Figure 7).leading to a decrease in the efficiency of the polymerization process.However, the increasing of the UV irradiation time up to 15 min partially reduces this effect, leading to a decrease in the swelling ratio to 18.9.Unless otherwise mentioned, the basic hydrogel composition consisted of the PAAm (15 wt.%) and the BIS-AAm (0.4 wt.%).In series, we varied: the BIS-AAm concentration (0.4 and 0.8 wt.% for Gel#1 and Gel#2, respectively); the time of the UV irradiation for Gel#2 from 5 to 10 min (the latter is Gel#3); the concentration of the {Mo 132 } (0.5 and 1.0 g•L −1 for Gel#4 and Gel#5, respectively); the absence and the presence of the TiO 2 nanoparticles (Gel#6 and Gel#7, respectively); and the aging of the TiO 2 -embedded hydrogel under 7 h of the UV irradiation (Gel#8).Because the addition of the TiO 2 nanoparticles into the PAAm@Mo 132 hydrogel results in the elongation of time under UV irradiation (up to 15 min) needed to complete the gelation process, we made the reference hydrogel PAAm@Mo 132 , which was obtained under the same condition as PAAm@Mo 132 @TiO 2 ; this is Gel#6.For this hydrogel series, we measured the equilibrium swelling ratio (Figure 8).As one can see, despite the double BIS-AAm concentration for Gel#2, the duration of the UV irradiation affects the swelling ratio more than BIS-AAm content as compared to Gel#1, giving the swelling ratio of 12.9, 38.3 and 13 for Gel#1, Gel#2 and Gel#3, respectively.In contrast, the exposition time under UV light was kept on the same level, but the introduction of {Mo 132 } resulted in the growth of the swelling ratio from 12.9 (Gel#1) to 34.2 and 44 (for Gel#4 and Gel#5, respectively).There are two possible reasons: (i) Keplerate {Mo 132 } works as optical filter decreasing the UV light flow leading to reduction in the polymerization rate; (ii) {Mo 132 } can trap the free radicals produced on B2 or monomer species, leading to a decrease in the efficiency of the polymerization process.However, the increasing of the UV irradiation time up to 15 min partially reduces this effect, leading to a decrease in the swelling ratio to 18.9.
decrease in the swelling ratio to 18.9.When the TiO2 nanoparticles were added to the PAAm/BIS-AAm/B2 mixture under neutral pH, we expected the effective polymerization under 365 nm light.Instead, the hydrogel structure was not formed; only small aggregates appeared.This result is opposite to the suggested protocol, where the only main difference in PAAm hydrogel preparation is the acidic pH [22].It is well known that Brönsted acid/base sites (as well as Lewis acid centers) on the TiO2 surface significantly affect the ROS generation process [30,31].Moreover, the hydrogen bond formation led to the preferable •OH radicals' generation on the {001} surfaces of anatase TiO2 [32].Therefore, the acidic environment can change the polymerization rate, leading to the hydrogel formation.However, even under neutral pH, When the TiO 2 nanoparticles were added to the PAAm/BIS-AAm/B2 mixture under neutral pH, we expected the effective polymerization under 365 nm light.Instead, the hydrogel structure was not formed; only small aggregates appeared.This result is opposite to the suggested protocol, where the only main difference in PAAm hydrogel preparation is the acidic pH [22].It is well known that Brönsted acid/base sites (as well as Lewis acid centers) on the TiO 2 surface significantly affect the ROS generation process [30,31].Moreover, the hydrogen bond formation led to the preferable •OH radicals' generation on the {001} surfaces of anatase TiO 2 [32].Therefore, the acidic environment can change the polymerization rate, leading to the hydrogel formation.However, even under neutral pH, the UV-light-assisted hydrogel formation occurred when {Mo 132 } was added.The inhibition of the hydrogel formation in the PAAm/BIS-AAm/B2 mixture with the TiO 2 nanoparticles can be explained through the two hypothesizes.First, due to the fast polymerization near the TiO 2 nanoparticles, the hydrogel network does not have time to form throughout all the volume.As a result, there is no homogeneous distribution of TiO 2 in the volume and the isolated TiO 2 -embedded polymeric aggregates appear.Another explanation relates to the quenching of the radical species generated from riboflavin molecules under the UV irradiation.This quenching can occur through recombination of riboflavin's or vinyl's radicals with ROS species arising from the TiO 2 surface.Therefore, being added, the Keplerate {Mo 132 } works as an optical filter decreasing the UV-light fraction absorbed by the TiO 2 , which makes the polymerization more pronounced (vide infra).Interestingly, the addition of the {Mo 132 } into the PAAm/BIS-AAm/B2 mixture increases the gelation time because of either the optical filter effect or free radical scavenging [23].The gelation of the PAAm/BIS-AAm/B2 mixture with {Mo 132 } and TiO 2 gives the drastic growth of the swelling ratio from 18.9 (Gel#6) to 80.7 (Gel#7).During the aging process under UV irradiation for 7 h, the swelling ratio of the Gel#7 reduced to 12.7 (Gel#8).
In accordance with the measured swelling ratio, we calculated the mesh size of the produced PAAm hydrogels.Based on the Flory-Rehner theory [33] for polymer networks, we estimated the number of monomeric residues (N C ) in the sub-chains between the crosslinks of the networks: where the V 1 , V 2 are molar volumes of a solvent (water) and a polymer, respectively; α is the equilibrium swelling ratio; χ is the Flory-Huggins parameter for the polymer-solvent mixture.We used V 1 = 18 cm 3 •mol −1 (water), V 2 = 56.2cm 3 •mol −1 (polyacrylamide) and χ = 0.12 [34].This equation gives Nc value under the assumption that polymeric sub-chains of the network are fully stretched.However, in the polyacrylamide gel, the sub-chains are statistically coiled.The mean square end-to-end distance <R 2 >, between the ends of these random Gaussian coils, which corresponds to the distance between adjacent cross-links, can be calculated according to the equations: where N is the number of bonds in the polymeric chain (it is two times larger than the N C value, as it includes the bonds in monomer units and bonds between them); a is the bond length; θ is the bond angle; and ϕ the angle of the hindered rotation.We took a = 0.154 nm for the ordinary C-C bond, θ = 109.5• and ϕ = 120 • .The calculated mesh size values (averaged R) are given in Table 1.The calculated mesh size values illustrate the above-mentioned trends.Addition of the {Mo 132 } two times increases the mesh size as compared to blank hydrogel Gel#1, but elongation of an exposition under the UV light from 10 to 15 min reduces the mesh size from 5 to 3 nm (Gel#4 and Gel#6, respectively).Despite the UV irradiation for 15 min, the mesh size enlarged significantly to 10.7 nm (Gel#7).Produced Gel#7 has a homogeneous distribution of the TiO 2 nanoparticles.After aging under the UV light for 7 h, the mesh size came to value similar to Gel#1.To check whether the presence of the {Mo 132 }, the TiO 2 and long-time UV irradiation influence on the PAAm network's molecular structure or not, we measured the IR spectra (Figure S7, see Supporting Information).It is clear that IR spectra of the hydrogels Gel#1, Gel#4, Gel#5 and Gel#8 (both irradiated front and nonirradiated back sides) are identical, practically.Only for Gel#8, the stretching of C-H bond near 2925 and 2854 cm −1 (asymmetric and symmetric, respectively) becomes sharper and more intense.
The role of TiO 2 is not so clear.Of course, the TiO 2 nanoparticles in mixture can scatter the UV light during the gelation to obtain the hydrogel with the larger mesh size.To be scattered, UV light should interact with the TiO 2 surface.Therefore, the main question concerns whether the radical species generated onto the TiO 2 surface accelerate the polymerization or quench it through the recombination.Because the simultaneous presence of the {Mo 132 } and the TiO 2 leads to increase both the gelation time and the mesh size decreasing the cross-link frequency, one can assume that the TiO 2 downshifts the polymerization rate in neutral pH condition when adsorbing the UV photons.Thus, serving as optical filter, the {Mo 132 } prevents the TiO 2 nanoparticles from the UV light absorption.

Photostability of PAAm@Mo 132 @TiO 2
To deepen understanding the role of the TiO 2 nanoparticles and to evaluate the photostability of the {Mo 132 }-embedded hydrogel, we studied the aging process during the long-time UV irradiation.The PAAm@Mo 132 and PAAm@Mo 132 @TiO 2 hydrogels were simultaneously exposed under the UV light (365 nm, 9 W) in two regimes: in the cell, where gel was located between two quartz windows and in the aqueous solution.In the former case, the dynamic oxygen exchange between an atmosphere and the hydrogel was absent and the swelling process was restricted by the size of the cell.In the case of an aqueous solution, both above-mentioned processes occurred in opposite fashion.In Figure 9, the comparison of the Gel#6 and Gel#7 after UV irradiation in the cell and an aqueous solution is presented.As one can see, the UV irradiation of PAAm@Mo 132 hydrogel in both regime (7 h in the cell and 1 h in an aqueous solution, Figure 9a,e,f and Supplementary Figures S8 and S9, see Supporting Information) results in the complete bleaching of the {Mo 132 }.This is expected behavior because the Keplerate {Mo 132 } can gradually decomposes in an aqueous media due to the oxidation of the Mo V -centers accelerating by even the visible light irradiation [19,35,36].
appeared on the top of the front side.We repeated this experiment and reduced the distance between the UV LED and the cell from 25 mm to the minimal value, 15 mm.The PAAm@Mo132@TiO2 was irradiated for 4.5 h, kept in the dark for 17 h (to the relaxation of the intrinsic hydrogel structure) and then irradiated again for 4.5 h.After a total of 9 h of exposition under UV light, the layered structure reproduced, but the top white layer became more pronounced from the front side.Moreover, due to the long-time irradiation by UV LED placed in proximity to the cell, the hydrogel's back side was more cross-linked.When removed from the cell, the back side of Gel#8 was concaved, as compared to the front side irradiated with UV light (Figure S11c-f, see Supporting Information).Contrary to the case of the PAAm@Mo132, the observed PAAm@Mo132@TiO2 hydrogel's behavior corresponds to presence of the competitive processes affecting the polymerization under UV light.In addition to the radicals' generation from the riboflavin molecules and the TiO2 surface, as well as the {Mo132} oxidative decomposition, one more process can exist, which is the photoinduced cascade of the electron transfer to the {Mo132}, preventing its structure from an oxidative decomposition (Figure 10).The images of the PAAm@Mo132@TiO2 (left) and PAAm@Mo132 (right) hydrogels after UV irradiation for 7 h in the cell.(b,c) The images of the broken PAAm@Mo132@TiO2 and PAAm@Mo132 hydrogels after UV irradiation for 7 h, respectively.(d) The image of the PAAm@Mo132@TiO2 hydrogel (after UV irradiation for 9 h) cut in half, the back side is concaved.(e,f) The images of of the PAAm@Mo132@TiO2 (left) and PAAm@Mo132 (right) hydrogels before (e) and after (f) UV irradiation for 3 h in the aqueous solution.
Even if we consider the random reflection of the UV light making possible some small flare of the hydrogel back side, the photon flux is incomparably bigger on the front side, where the polymerization degree should be higher.To explain the observed inversion of the cross-links frequency, we should mention that after the exposition of the liquid mixture under the UV light for 15 min to obtain the hydrogel, there is an unreacted amount The images of the PAAm@Mo 132 @TiO 2 (left) and PAAm@Mo 132 (right) hydrogels after UV irradiation for 7 h in the cell.(b,c) The images of the broken PAAm@Mo 132 @TiO 2 and PAAm@Mo 132 hydrogels after UV irradiation for 7 h, respectively.(d) The image of the PAAm@Mo 132 @TiO 2 hydrogel (after UV irradiation for 9 h) cut in half, the back side is concaved.(e,f) The images of of the PAAm@Mo 132 @TiO 2 (left) and PAAm@Mo 132 (right) hydrogels before (e) and after (f) UV irradiation for 3 h in the aqueous solution.
In the case of the PAAm@Mo 132 @TiO 2 hydrogel, the irradiation in the cell or an aqueous solution gives different results.In the aqueous solution, after an exposition under UV light for 3 h, the irradiated (front) side of the hydrogel became white colored, demonstrating the homogeneously distributed TiO 2 nanoparticles within the gel matrix (Figures S9 and S10, see Supporting Information).Irrespective of this, the nonirradiated (back) side remained the brown color, indicating the {Mo 132 } preservation but with decreased concentration (Figure S10, see Supporting Information).Due to the fast decomposition of the {Mo 132 } under UV light from the front side, the remained TiO 2 nanoparticles effectively scatter the light protecting the back side from the irradiation and the {Mo 132 } from the decomposition, consequently.Despite the ROS generation on the TiO 2 nanoparticles under the UV light, these oxygen radicals cannot penetrate into the hydrogel network to reach the {Mo 132 } on the back side owing to the short lifetime and the extremely small diffusion coefficient of the ROS [29].In contrast, being irradiated by the UV light in the cell for 7 h, the PAAm@Mo 132 @TiO 2 hydrogel became white on the back side (Figure 9b), whereas the {Mo 132 } still existed on the front side, and only a very thin white layer without {Mo 132 } appeared on the top of the front side.We repeated this experiment and reduced the distance between the UV LED and the cell from 25 mm to the minimal value, 15 mm.The PAAm@Mo 132 @TiO 2 was irradiated for 4.5 h, kept in the dark for 17 h (to the relaxation of the intrinsic hydrogel structure) and then irradiated again for 4.5 h.After a total of 9 h of exposition under UV light, the layered but the top white layer became more pronounced from the front side.Moreover, due to the long-time irradiation by UV LED placed in proximity to the cell, the hydrogel's back side was more cross-linked.When removed from the cell, the back side of Gel#8 was concaved, as compared to the front side irradiated with UV light (Figure S11c-f, see Supporting Information).Contrary to the case of the PAAm@Mo 132 , the observed PAAm@Mo 132 @TiO 2 hydrogel's behavior corresponds to presence of the competitive processes affecting the polymerization under UV light.In addition to the radicals' generation from the riboflavin molecules and the TiO 2 surface, as well as the {Mo 132 } oxidative decomposition, one more process can exist, which is the photoinduced cascade of the electron transfer to the {Mo 132 }, preventing its structure from an oxidative decomposition (Figure 10).The presence of {Mo132} cannot totally exclude the fact that the TiO2 nanoparticles adsorb a small fraction of UV light across whole hydrogel.In accordance with the energetic levels diagram, the conduction band (CB) of the TiO2 lies above the LUMO state of the {Mo132} on the 0.19 eV [37], making possible the spontaneous electron transfer [38].In Even if we consider the random reflection of the UV light making possible some small flare of the hydrogel back side, the photon flux is incomparably bigger on the front side, where the polymerization degree should be higher.To explain the observed inversion of the cross-links frequency, we should mention that after the exposition of the liquid mixture under the UV light for 15 min to obtain the hydrogel, there is an unreacted amount of the AAm and BIS-AAm molecules.If the cross-links frequency becomes higher on the back side after 7-9 h of UV irradiation, it means that polymerization rate is faster here.Therefore, during the UV irradiation, in the hydrogel front part the preservation of {Mo 132 } decreases the polymerization rate owing to the optical filter effect of the Keplerate (see absorption spectrum in Figure 10).
The presence of {Mo 132 } cannot totally exclude the fact that the TiO 2 nanoparticles adsorb a small fraction of UV light across whole hydrogel.In accordance with the energetic levels diagram, the conduction band (CB) of the TiO 2 lies above the LUMO state of the {Mo 132 } on the 0.19 eV [37], making possible the spontaneous electron transfer [38].In its turn, the TiO 2 needs a sacrificial electron donor, which can be oxidized by the TiO 2 ground state (valence band, VB).The unreacted acrylamide molecules are good candidates for that, but the HOMO state of AAm is 9.8 eV, based on the Ultraviolet Photoelectron Spectroscopy [39], and the LUMO state is −5.52 eV in accordance with band gap (4.28 eV) determined by means of Tauc's plot (Figure S12, see Supporting Information).However, it is well known that the surface electronic state can be different from the bulk of the material.Furthermore, the ab initio calculation demonstrates that above the VB level (near 6 eV, e.g.) novel electronic states appear due to adsorption of the molecular species on the TiO 2 surface [40].Even more, under UV light on the TiO 2 surface, the produced superoxide radicals O 2 •-possess the inversed HOMO-SOMO states with −5.63 eV for HOMO level.Therefore, the ad-molecules and ad-atoms can serve as the electron donors on the TiO 2 surface.
The intensification of the photoinduced cascade of the electron transfer to the {Mo 132 } on the front side (where the photon flux is much higher) can explain the competition between this process and polymerization hindered by the presence of {Mo 132 }.Thus, the TiO 2 behaves like a mediator of the electron transfer to {Mo 132 }.This transfer occurs on the nanoparticles' surface, providing the simultaneous presence of the sacrificial donor and Keplerate at the optimal distance.

TiO 2 Characterization
As a photocatalyst, we used commercial titanium dioxide P25 grade purchased from Evonik.The specific surface area of it (45.2m 2 /g) was determined via low-temperature equilibrium sorption of nitrogen (BET equation) using a Micromeritics TriStar 3020 vacuum sorption unit.Electron microscope photographs of the powder were obtained with a JEOL JEM 2100 transmission electron microscope (TEM) (Figure S1a, see Supporting Information).The particle size distribution was determined by the graphical analysis of the micrographs (Figure S1b, see Supporting Information).The particles were considered spherical in order to calculate the average particle size.Number average particle diameter (d n ) was 39 nm, while weighted average diameter (d w ) was 115 nm.The considerable difference between these two values indicated that the particle size distribution was broad.The phase composition of titanium dioxide was characterized via X-ray phase analysis on a Bruker D8 Discover diffractometer using CuK α band and a graphite monochromator on a diffracted beam.Data were processed using the TOPAS 2.1 program with Ritveld's refinement of the parameters.The anatase phase, the content of which was 88 wt.%, was found to predominate (CSR size = 25 nm, lattice parameters: a = 3.786 Å, c = 9.507 Å).The rutile phase (12 wt.%) was detected in addition to the anatase phase (CSR size = 36 nm, lattice parameters: a = 4.594 Å, c = 2.959 Å).The effective band gap of titanium dioxide (3.38 eV) was determined optically [41] from the diffuse reflectance spectrum in 190-1400 nm interval obtained using a UV-2600 Shimadzu spectrophotometer with an ISR-2600 Plus integrating sphere attachment (Figure S1c, see Supporting Information).
First, the stock aqueous solution (gel precursor) of AAm, BIS-AAm, Alg-Na and TEMED was prepared.The weighted amount of TiO 2 was dispersed in water under ultrasound treatment using "Cole Palmer CPX 750" Ultrasonic Homogenizer (at 150 W output power for 15 min).Then, TiO 2 suspension was mixed with stock solution with vigorous stirring.The final concentration of TiO 2 suspension is 1 g•L −1 .The content of AAm in relation to Alg-Na varied from 50 to 95 wt.%.At the same time, the mass fraction of the {Aam + Alg-Na} mixture in the stock solution remained always the same-10 wt.%.In the reaction mixture, the final concentration of BIS-AAm and TEMED was 25 and 8.8 mM.
Further, the reaction mixture (gel precursor) was added dropwise through a needle into an aqueous solution containing 0.05 M of APS and 0.5 M of calcium chloride (Figure S2, see Supporting Information).As a result, hydrogel spherical beads instantly precipitated, forming elastic gel, Alg-Ca@PAAm@TiO 2 .The resulted product was kept in a saline solution for 24 h to finalize the ion exchange and complete the acrylamide polymerization process, after the hydrogel was washed in distilled water (in static regime) for two weeks to achieve equilibrium swelling.Diameter of beads are ca.3 mm.Synthetic procedure is presented in Figure 1.

Swelling Behavior of Alg-Ca@PAAm@TiO 2 Beads
Then, the resulting hydrogel beads were washed for two weeks in distilled water with daily water renewal to remove residual monomers and to achieve an equilibrium swelling ratio (α), which was determined gravimetrically using the following equation: where m g is the mass of a swollen gel and m 0 is the mass of a gel dry residue after complete evaporation of water, which was conducted at 70 • C until a constant weight was reached.
In the case of composite gels, the swelling ratio determined according to Equation (6) gives the water content related to the combined mass of polymer and solid particles (TiO 2 ), although the latter do not swell.Thus, the swelling ratio of the polymeric network alone (α') was used for their characterization.It is related to the overall swelling ratio α according to the following equation: where m(TiO 2 ) is the mass of titanium dioxide nanoparticles in the reaction mixture and m net is the combined mass of monomers and cross-linker in the synthesis.

Photocatalytic Activity
In the photocatalytic activity experiments, the hydrogel samples were previously kept for 24 h in the dark in 10 mL of 3 µM aqueous solution of methyl orange until equilibrium saturation of the hydrogel with the dye was reached.After that, the gel beads were placed in the cylindrical quartz cuvette arranged in the laboratory setup with UV irradiation (LED).The sketch of the setup is given in Figure S3 (Supporting Information).The emis-sion spectrum of LED has maximum at 365 nm, and the light flux intensity is 16 mW•cm −2 , as measured by thermopile photometer Thorlabs PM16-401.During the UV irradiation, the bleaching of the dye aqueous solution was observed due to the decomposition of the dye molecules by gel photocatalyst.At certain time intervals of irradiation, the optical density of the aqueous solution in cuvette was measured spectrophotometrically in situ by an optical fiber probe (Thorlabs Transmission Dip Probe TP22) connected to the spectrophotometer (Thorlabs CCS 100) at a wavelength of 464 nm.The measurements were carried out at 25 • C under the permanent stirring of the solution with an overhead stirrer.The spectrophotometer was preliminarily calibrated for the concentration of methyl orange in an aqueous solution at a wavelength of 464 nm.
3.5.Photostability of the Hydrogel Beads Alg-Ca@PAAm@TiO 2 The photostability of the gels under UV irradiation (365 nm, light flux is 16 mW•cm −2 ) was determined by the gravimetric method.Since the hydrogel is a two-component system that consists of the polymer network and the bound water, the weight loss may be due to a decrease in at least one of them.The change in the mass of dried gels before and after UV irradiation characterizes the photostability of gel's polymer matrix: where m 0 and m 0 (UV) is the mass of dried gel before and after UV-irradiation, respectively.UV-irradiation of gel's samples was carried out during 5 h in thermostatically ventilated beakers with 10 mL of water.

Thermodynamic Study of the "Alginate-TiO 2 " System
The enthalpy of adhesion of the alginate polymer chains to the surface of the TiO 2 particles was determined using a thermochemical cycle.Model polymer composites with the load of solid filler ranging from 0 to 100 wt.% were prepared separately as follows.A weighed portion of the TiO 2 powder was placed in this solution of sodium alginate (Alg-Na) and mechanically stirred in an agate mortar.The obtained homogeneous mixture was dried on a Teflon support to a constant weight.A weighed portion of the composite was placed in a 0.35 mL thin-walled glass ampoule and dried in a vacuum to constant weight; thereafter, the ampoule was sealed.Then, the ampoule was placed in a calorimetric cell with 5 mL of water.The thermal effects of dissolution in water of filled polymer composites and the heats of wetting of the TiO 2 particles with water were measured using a Calvet microcalorimeter of a laboratory design [42] kept at 298 ± 0.1 K.During the calorimetric experiment, the ampoule was broken inside of the cell and the thermal effect was recorded.The absolute uncertainty of the calorimetric measurements, according to the electrical calibration data, was ±0.02 J.

Hydrogel PAAm@TiO 2 @Mo 132 Synthesis
The {Mo 132 } crystal were produced via standard protocol [18].Their structures were confirmed by FT-IR (Nicolet 6700, Thermo Scientific, Waltham, MA, USA) and Raman spectroscopy (Alpha 300 AR confocal Raman microscope, 633 nm laser, WiTec GmbH, St. Johann, Austria) and comparison with the literature [18,43]  For the hydrogel production, the following protocol was used.The stock solution (1) was prepared by dissolving acrylamide 0.75 g (Merck) and N,N-methylenebisacrylamide 0.02 g (Sigma-Aldrich, Burlington, NJ, USA, ≥99%) in 1.730 mL of the riboflavin (vitamin B2, Sigma-Aldrich, ≥98%) aqueous solution (0.06 g•L −1 , freshly prepared) in vial under gentle heating on the plate (80-100 • C set temperature).The vial was closed with a silicon cap and covered by the aluminum foil to keep the solution in dark.To prepare the stock solution (2), 0.0025 g of {Mo 132 } was dissolved in 2 mL of water.After that, 0.5 mL of riboflavin solution (0.06 g•L −1 , freshly prepared) was added.In the case of TiO 2 -embedded hydrogel, the TiO 2 P25 powder 0.005 g (Evonik) was added along with {Mo 132 } in 2 mL of water followed with ultrasonication for 10 min.
Then 1.25 mL of stock solution (1) was kept under 50 • C for 10 min (in dark) and irradiated with 365 nm UV-light (18 W, LED) for 15 s to reach the pre-gelation.Immediately after that, the 1.25 mL of stock solution (2) was added and mixed with pipette.Further, mixture was transferred in the cell consisted of polyvinylchloride (PVC) ring (thickness is 3.8 mm, inner hole diameter is 19 mm) placed between two fused quartz windows.Cell was located in tight contact with two UV-LED sets (9 W per each window) and irradiated during 10 or 15 min (depended on experiment condition) while cooling with fan.
3.8.Swelling Behavior of PAAm@Mo 132 and PAAm@TiO 2 @Mo 132 Swelling behavior was studied the same way as previously described.Briefly, we kept hydrogel in 250 mL of distilled water for 10 days until reaching the constant weight.After swelling was completed, the hydrogel was dried in vacuo at 60 • C on rotary evaporator.The swelling ratio (α) and its corrected value (α') were calculated in accordance with Equations ( 6) and (7), respectively.
3.9.Photostability of PAAm@Mo 132 and PAAm@TiO 2 @Mo 132 When hydrogel was formed in the cell, the photostability was studied in two variants (Figure S4, see Supporting Information): (i) the long-time (7-9 h) irradiation in the cell only from one side with UV (365 nm) LED (9 W per side), distance is 25 mm or 15 mm; (ii) the obtained hydrogel in PVC-ring was placed in water and irradiated only from one side with UV (365 nm) LED (9 W) for 3 h.

UV-Vis Spectroscopy
The UV-Vis spectra of the aqueous solution of the acrylamide and {Mo 132 } were measured by the spectrometers PB-2201 (Solar Lab, Minsk, Belarus) and UV-1800 (Shimadzu, Kyoto, Japan) in the fused quartz cuvette.

Conclusions
In this work, the two types of PAAm-based hydrogels were produced.The hydrogel network structure was designed by means of the organic (alginate) or the inorganic (Keplerate {Mo 132 }) polyelectrolytes.Using the Alg-Ca and TiO 2 nanoparticles, we controlled the shape and photocatalytic activity of the PAAm-based hydrogel beads, whereas the addition of {Mo 132 } allowed us to produce the photopolymerized biocompatible hydrogel.
In accordance with swelling behavior of the Alg-Ca@PAAm@TiO 2 hydrogels, we found that the swelling ratio increases drastically when PAAm content reaches the 90 wt.%.To evaluate the contribution of the TiO 2 nanoparticle into the swelling behavior, the enthalpy of dissolution was measured.It clearly showed that both alginate and PAAm macromolecules have no thermodynamic benefit to interact with the TiO 2 surface, making it accessible to molecules' adsorption and ROS generation.The produced Alg-Ca@PAAm@TiO 2 hydrogel beads demonstrated the high photocatalytic performance towards the methyl orange dye bleaching in the aqueous solution.For the Alg-Ca@PAAm@TiO 2 hydrogels with PAAm contents of 50, 70, 80 and 90 wt.%, the found values of the effective kinetic constants k were: 0.2 × 10 −3 , 0.7 × 10 −3 , 1.5 × 10 −3 and 2.6 × 10 −3 min −1 , respectively.In addition, we studied the photostability and re-swelling behavior of the Alg-Ca@PAAm@TiO 2 hydrogel beads that is strictly important for actual photocatalytic application.As we found, the introduction of PAAm at 90 wt.% remains the re-swelling ratio on 60% from the initial

Figure 3 .
Figure 3. (a) The dissolution enthalpy of Alg-Na@TiO2 composites and (b) the dependency of the interfacial enthalpy (ΔHint) on the TiO2 weight fraction in the Alg-Na polymer matrix.

Figure 3 .
Figure 3. (a) The dissolution enthalpy of Alg-Na@TiO 2 composites and (b) the dependency of the interfacial enthalpy (∆H int ) on the TiO 2 weight fraction in the Alg-Na polymer matrix.

Figure 4 .
Figure 4. Re-swelling ability of the dried Alg-Ca@PAAm@TiO2 hydrogel beads with various PAAm content.The insert presents the re-swelling process ability of the dried Alg-Ca@PAAm@TiO2 hydrogel beads with 50 wt.% of PAAm.

Figure 4 .
Figure 4. Re-swelling ability of the dried Alg-Ca@PAAm@TiO 2 hydrogel beads with various PAAm content.The insert presents the re-swelling process ability of the dried Alg-Ca@PAAm@TiO 2 hydrogel beads with 50 wt.% of PAAm.

Figure 5 .
Figure 5. UV-degradation degree of the Alg-Ca hydrogel beads with (1) and without (2) embedded TiO2 nanoparticles after 5 h of the UV irradiation depended on the initial concentration of sodium alginate.The insert presents the UV-degradation of the Alg-Ca@TiO2 hydrogel beads produced at 0.5 wt.% of Alg-Na in aqueous solution.

Figure 5 .
Figure 5. UV-degradation degree of the Alg-Ca hydrogel beads with (1) and without (2) embedded TiO 2 nanoparticles after 5 h of the UV irradiation depended on the initial concentration of sodium alginate.The insert presents the UV-degradation of the Alg-Ca@TiO 2 hydrogel beads produced at 0.5 wt.% of Alg-Na in aqueous solution.

Figure 7 .
Figure 7.The scheme of the PAAm@Mo132@TiO2 hydrogel's synthesis via polymerization with riboflavin (vitamin B2) as the photoinitiator under the UV light irradiation.The {Mo132} structure is represented by the coordinative oxygen polyhedra (yellow).

Figure 7 .
Figure 7.The scheme of the PAAm@Mo 132 @TiO 2 hydrogel's synthesis via polymerization with riboflavin (vitamin B2) as the photoinitiator under the UV light irradiation.The {Mo 132 } structure is represented by the coordinative oxygen polyhedra (yellow).

Figure 7 .
Figure 7.The scheme of the PAAm@Mo132@TiO2 hydrogel's synthesis via polymerization with riboflavin (vitamin B2) as the photoinitiator under the UV light irradiation.The {Mo132} structure is represented by the coordinative oxygen polyhedra (yellow).

Figure 8 .
Figure 8.The swelling ratio for the PAAm (15 wt.%) hydrogels prepared under different conditions (see text for detail).Briefly, the differences between hydrogels are presented in figure.The purple frame corresponds to the hydrogel with embedded TiO2 and reference hydrogel.The dark-blue frame corresponds to the TiO2-embedded hydrogel after 7 h of UV irradiation.

Figure 8 .
Figure 8.The swelling ratio for the PAAm (15 wt.%) hydrogels prepared under different conditions (see text for detail).Briefly, the differences between hydrogels are presented in figure.The purple frame corresponds to the hydrogel with embedded TiO 2 and reference hydrogel.The dark-blue frame corresponds to the TiO 2 -embedded hydrogel after 7 h of UV irradiation.

Figure 9 .
Figure 9. (a)The images of the PAAm@Mo132@TiO2 (left) and PAAm@Mo132 (right) hydrogels after UV irradiation for 7 h in the cell.(b,c) The images of the broken PAAm@Mo132@TiO2 and PAAm@Mo132 hydrogels after UV irradiation for 7 h, respectively.(d) The image of the PAAm@Mo132@TiO2 hydrogel (after UV irradiation for 9 h) cut in half, the back side is concaved.(e,f) The images of of the PAAm@Mo132@TiO2 (left) and PAAm@Mo132 (right) hydrogels before (e) and after (f) UV irradiation for 3 h in the aqueous solution.

Figure 9 .
Figure 9. (a)The images of the PAAm@Mo 132 @TiO 2 (left) and PAAm@Mo 132 (right) hydrogels after UV irradiation for 7 h in the cell.(b,c) The images of the broken PAAm@Mo 132 @TiO 2 and PAAm@Mo 132 hydrogels after UV irradiation for 7 h, respectively.(d) The image of the PAAm@Mo 132 @TiO 2 hydrogel (after UV irradiation for 9 h) cut in half, the back side is concaved.(e,f) The images of of the PAAm@Mo 132 @TiO 2 (left) and PAAm@Mo 132 (right) hydrogels before (e) and after (f) UV irradiation for 3 h in the aqueous solution.

Inorganics 2023 , 20 Figure 10 .
Figure 10.The UV-Vis absorption spectrum of the aqueous solution of the {Mo132} with concentration is 5 × 10 −6 mol•L −1 and the energetic level diagram for the acrylamide, TiO2 and {Mo132} demonstrating the possible cascade of the photoinduced electron transfer.Blue arrows illustrate the electrons.

Figure 10 .
Figure 10.The UV-Vis absorption spectrum of the aqueous solution of the {Mo 132 } with concentration is 5 × 10 −6 mol•L −1 and the energetic level diagram for the acrylamide, TiO 2 and {Mo 132 } demonstrating the possible cascade of the photoinduced electron transfer.Blue arrows illustrate the electrons.

Table 1 .
The mesh size of the PAAm hydrogels calculated by means of Flory-Rehner theory.