Computing Brain White and Grey Matter Injury Severity in a Traumatic Fall

In the real world, the severity of traumatic injuries is measured using the Abbreviated Injury Scale (AIS). However, the AIS scale cannot currently be computed by using the output from finite element human computer models, which currently rely on maximum principal strains (MPS) to capture serious and fatal injuries. In order to overcome these limitations, a unique Organ Trauma Model (OTM) able to calculate the threat to the life of a brain model at all AIS levels is introduced. The OTM uses a power method, named Peak Virtual Power (PVP), and defines brain white and grey matter trauma responses as a function of impact location and impact speed. This research has considered ageing in the injury severity computation by including soft tissue material degradation, as well as brain volume changes due to ageing. Further, to account for the limitations of the Lagrangian formulation of the brain model in representing hemorrhage, an approach to include the effects of subdural hematoma is proposed and included as part of the predictions. The OTM model was tested against two real-life falls and has proven to correctly predict the post-mortem outcomes. This paper is a proof of concept, and pending more testing, could support forensic studies.


Introduction
Traumatic Brain Injuries (TBI) have been a major cause of morbidity and mortality for many years, and a major systematic review was conducted in 2005 [1], which is still one of the best-collected references on the subject. As shown in the review, TBIs are the leading cause of death between the ages of 15 and 44 throughout Europe [2]. In Southern Europe, road traffic crashes constitute the vast majority of cases and falls, mainly related to alcohol consumption, are the leading cause of trauma in Northern Europe [3]. Head trauma accounts for a majority of trauma deaths [4]. Some researchers predict that road traffic crash-related injuries will increase from ninth to third in the world disease burden ranking [5]. Another review by Post et al. [6] gives a highly detailed account of the history of TBI from the Wayne State Curve in the early 50's, through the Gadd Index leading to the Head Injury Criteria (HIC) in the US [7] and the Head Injury Prediction Criterion (HPC) in Europe [8].
These early linear acceleration based criteria have attracted much criticism over the intervening years [6,9], as they do not show high correlation with major parameters, and have led to more advanced models such as GAMBIT [10] and the Head Impact Power criterion [11]. It was shown in 2001 [12] that all theoretically derived criteria, as opposed to empirical criteria like HIC, etc., can be shown to be variants of the theory of Peak Virtual Power (PVP), which is used in this study.
There are a number of papers on TBI that mention falls; however, there are actually few that analyse head injury and compare the results to the real world and even less using Finite Element Table 1. Abbreviate Injury Scale (AIS) levels and risk to life [27].

AIS Level
Injury Risk of Death% The measurements from ATDs can only be used to speculate on the overall probability of death and have no internal organs, consequently they are not useful in predicting soft tissue injuries in a deterministic manner. Human-computer models, like the THUMS [26], have modelled the soft organ tissues (heart, kidneys, liver, spleen, liver, grey and white matter) and can output soft tissue Maximum Principal Strains (MPS). The MPS threshold considered in human-computer models are critical MPS values, which are supposed to represent severe injuries (AIS4), i.e., observable damage when physical human tissue tests are performed [28]. The MPS critical injury outputs levels, currently used by the scientific community when using Human-Computer models, are listed in Table 2. However, earlier work [29] has shown that using the THUMS model, in general, MPS significantly over-estimates the magnitude of the injury severity of the body organs; it is very diffuse and cannot predict the location of the injury, whereas PVP can address both these issues. Table 2. White and grey brain matter maximum principal strain tolerance limits [30,31].

Body Part
Load Threshold AIS Level Brain Contusion (Grey Matter) Maximum principal strain 26% [30] 3 Diffuse Axonal Injury (DAI) (White Matter) Maximum principal strain 21% [31] 4 Using MPS provides a major limitation to trauma computation analysis, as (a) it is only possible to capture if the AIS 4 is reached and (b) it is not possible to compute intermediate AIS levels below and higher than AIS 4.
MPS are standard outputs suggested by Human-Computer models (Table 2), which is a major limitation when investigating injury severity predictions. In this study, THUMS 4.01, using LS-Dyna as an explicit solver, was chosen because of its excellent level of calibration against cadaver tests, as well as its brain overall mesh density (5 mm), allowing the computation of a fall under 1 h on a quad-core laptop, whilst respecting a conservative energy balance, as illustrated in Figure 3. THUMS 4.01 is built for the purpose of human safety in transport applications. It has a macroscopic model of the brain to provide automotive design/analyst engineers, who have no medical background, with a tool to assess the risk to transport users. The model does not include blood vessels and fluid-structure interaction details, having discretised the brain with a 5 mm average element size. All the elements are Lagrangian and the contact is a global sliding contact. This discretisation has influenced the location of grey and white matter elements ( Figure 3); however, as the material properties of grey and white matter are identical in the THUMS 4.01 model, any meshing discrepancies are overcome in the post-processing stage, where the user can make the conscious decision to attribute the trauma value to the actual grey and white matter zones, which this research has done. Figure 1a illustrates the THUMS 4.01 mesh distribution. Note that the same discrepancy is also present in more complex head models, like the GHBMC (Figure 1b). critical MPS values, which are supposed to represent severe injuries (AIS4), i.e., observable damage when physical human tissue tests are performed [28]. The MPS critical injury outputs levels, currently used by the scientific community when using Human-Computer models, are listed in Table 2. However, earlier work [29] has shown that using the THUMS model, in general, MPS significantly over-estimates the magnitude of the injury severity of the body organs; it is very diffuse and cannot predict the location of the injury, whereas PVP can address both these issues. Using MPS provides a major limitation to trauma computation analysis, as (a) it is only possible to capture if the AIS 4 is reached and (b) it is not possible to compute intermediate AIS levels below and higher than AIS 4. Table 2. White and grey brain matter maximum principal strain tolerance limits [30,31]. MPS are standard outputs suggested by Human-Computer models ( Table 2), which is a major limitation when investigating injury severity predictions. In this study, THUMS 4.01, using LS-Dyna as an explicit solver, was chosen because of its excellent level of calibration against cadaver tests, as well as its brain overall mesh density (5 mm), allowing the computation of a fall under 1 h on a quadcore laptop, whilst respecting a conservative energy balance, as illustrated in Figure 3. THUMS 4.01 is built for the purpose of human safety in transport applications. It has a macroscopic model of the brain to provide automotive design/analyst engineers, who have no medical background, with a tool to assess the risk to transport users. The model does not include blood vessels and fluid-structure interaction details, having discretised the brain with a 5 mm average element size. All the elements are Lagrangian and the contact is a global sliding contact. This discretisation has influenced the location of grey and white matter elements ( Figure 3); however, as the material properties of grey and white matter are identical in the THUMS 4.01 model, any meshing discrepancies are overcome in the post-processing stage, where the user can make the conscious decision to attribute the trauma value to the actual grey and white matter zones, which this research has done. Figure 1a illustrates the THUMS 4.01 mesh distribution. Note that the same discrepancy is also present in more complex head models, like the GHBMC (Figure 1b).  THUMS 4.01 is very efficient as the model contains one general global single surface contact, while the GHBMC has in excess of 400 contacts, which has the consequence of increasing the runtime  [26] vs. GHBMC brain meshed model (b) [25]. (a) THUMS brain model, (b) GHBMC brain model (cross-section). THUMS 4.01 is very efficient as the model contains one general global single surface contact, while the GHBMC has in excess of 400 contacts, which has the consequence of increasing the runtime because of the contact search algorithms. The skull is composed of 3 separate layers, which represent the diploe and the internal and external tables [30], as illustrated in Figure 2. because of the contact search algorithms. The skull is composed of 3 separate layers, which represent the diploe and the internal and external tables [30], as illustrated in Figure 2. Cross-section of the skull (THUMS [26] vs. in a human specimen [30,32]).

Body
It can be observed in Figure 3 that the total energy is constant during the computation of a fall with a negligible rise of hourglass energy, showing that no element locking is present and that all the contacts inside the brain model are working as intended. Injury severity can be computed from a concept called Peak Virtual Power (PVP). Peak Virtual Power is based on the general principle of the second law of thermodynamics, stating that entropy (state of disorder) increases after each mechanical process [12,33,34]. When a collision takes place, the entropy (represented by PVP) always increases, never to return. A typical pattern of this behaviour is illustrated in Figure 4, power goes up and down, while PVP always keeps to the maximum value at all time, PVP is proportional to the maximum rate of entropy production and as Schrodinger said, "a body reaches a maximum state of entropy, which is death" [35].  [26] vs. in a human specimen [30,32]).
It can be observed in Figure 3 that the total energy is constant during the computation of a fall with a negligible rise of hourglass energy, showing that no element locking is present and that all the contacts inside the brain model are working as intended.
Math. Comput. Appl. 2020, 25, x FOR PEER REVIEW 4 of 18 because of the contact search algorithms. The skull is composed of 3 separate layers, which represent the diploe and the internal and external tables [30], as illustrated in Figure 2.
It can be observed in Figure 3 that the total energy is constant during the computation of a fall with a negligible rise of hourglass energy, showing that no element locking is present and that all the contacts inside the brain model are working as intended. Injury severity can be computed from a concept called Peak Virtual Power (PVP). Peak Virtual Power is based on the general principle of the second law of thermodynamics, stating that entropy (state of disorder) increases after each mechanical process [12,33,34]. When a collision takes place, the entropy (represented by PVP) always increases, never to return. A typical pattern of this behaviour is illustrated in Figure 4, power goes up and down, while PVP always keeps to the maximum value at all time, PVP is proportional to the maximum rate of entropy production and as Schrodinger said, "a body reaches a maximum state of entropy, which is death" [35]. Injury severity can be computed from a concept called Peak Virtual Power (PVP). Peak Virtual Power is based on the general principle of the second law of thermodynamics, stating that entropy (state of disorder) increases after each mechanical process [12,33,34]. When a collision takes place, the entropy (represented by PVP) always increases, never to return. A typical pattern of this behaviour is illustrated in Figure 4, power goes up and down, while PVP always keeps to the maximum value at all time, PVP is proportional to the maximum rate of entropy production and as Schrodinger said, "a body reaches a maximum state of entropy, which is death" [35].  PVP [12,33] is derived using the Clausius-Duhem inequality, which is from the rate-dependent form of the second law of thermodynamics [37], illustrated in Equation (1). PVP [12,33] is derived using the Clausius-Duhem inequality, which is from the rate-dependent form of the second law of thermodynamics [37], illustrated in Equation (1). where: For a mechanical system, assuming the contribution of elasticity and heat flux are small, the constitutive relationships are: With epsilon the elastic and plastic tensor, T the temperature and D the damage tensor. Equation (2) is differentiated (Equation (3)) and reinserted in Equation (1) to give Equation (4): The Clausius-Duhem inequality now becomes: Hence: It is assumed that the Damage Tensor is proportional to Injury Severity or AIS [33]. Following the fact that entropy keeps on increasing during a collision, Equation (6) is derived, illustrating the trauma process from Figure 4.
Consequently, it is possible the calculate the peak power inside an organ and compare it to the critical level observed in Table 2 and calculate the maximum power needed to observed AIS 4 on the white matter and AIS 3 on the grey matter. To date, according to the authors, no literature has clearly documented the actual values MPS level relating to AIS 4 and ageing. Consequently, the current research has used the MPS data available and accepted in the THUMS model.
It has been observed in real life that there was a cubic relationship between the threat to life and AIS [36], shown in Figure 5.
Math. Comput. Appl. 2020, 25, x FOR PEER REVIEW 6 of 18 documented the actual values MPS level relating to AIS 4 and ageing. Consequently, the current research has used the MPS data available and accepted in the THUMS model. It has been observed in real life that there was a cubic relationship between the threat to life and AIS [36], shown in Figure 5.
It is, therefore possible to extract the intermediate AIS levels from Table 1. As an example, if the PVP necessary to cause a severe injury is known (AIS 4) then it is possible to extract how much PVP the organ can withstand to reach AIS 1, 2, 3 and 5. The PVP values can be scaled from AIS 4 by the ratios 1/64 (1 3 /4 3 ), 8/64 (2 3 /4 3 ), 27/64 (3 3 /4 3 ) and 125/64 (5 3 /4 3 ), respectively, to create the full map of trauma injuries for that organ, creating an "Organ Trauma Model" (OTM). It should be noted that AIS is ordinal, and so the interpolation model is only interrogated at ordinal values.
As an illustration, any OTM, will be therefore represented by a graph containing the relationship between PVP, impact velocity and AIS, as illustrated in Figure 6. It has been possible to include error corridors (upper and lower) for each AIS value by considering the spread of data from Figure 5. As an example, looking at Figure 6, Table 3 illustrates trauma severity scenarios. Relationship between Threat to Life (%) and AIS (Baker [38], CCIS [39], NASS [40], and Walder [41]).
It is, therefore possible to extract the intermediate AIS levels from Table 1. As an example, if the PVP necessary to cause a severe injury is known (AIS 4) then it is possible to extract how much PVP the organ can withstand to reach AIS 1, 2, 3 and 5. The PVP values can be scaled from AIS 4 by the ratios 1/64 (1 3 /4 3 ), 8/64 (2 3 /4 3 ), 27/64 (3 3 /4 3 ) and 125/64 (5 3 /4 3 ), respectively, to create the full map of trauma injuries for that organ, creating an "Organ Trauma Model" (OTM). It should be noted that AIS is ordinal, and so the interpolation model is only interrogated at ordinal values.
As an illustration, any OTM, will be therefore represented by a graph containing the relationship between PVP, impact velocity and AIS, as illustrated in Figure 6. It has been possible to include error corridors (upper and lower) for each AIS value by considering the spread of data from Figure 5.
Math. Comput. Appl. 2020, 25, x FOR PEER REVIEW 6 of 18 documented the actual values MPS level relating to AIS 4 and ageing. Consequently, the current research has used the MPS data available and accepted in the THUMS model. It has been observed in real life that there was a cubic relationship between the threat to life and AIS [36], shown in Figure 5.
It is, therefore possible to extract the intermediate AIS levels from Table 1 As an illustration, any OTM, will be therefore represented by a graph containing the relationship between PVP, impact velocity and AIS, as illustrated in Figure 6. It has been possible to include error corridors (upper and lower) for each AIS value by considering the spread of data from Figure 5. As an example, looking at Figure 6, Table 3 illustrates trauma severity scenarios. As an example, looking at Figure 6, Table 3 illustrates trauma severity scenarios.  Extraction of injury severity has been attempted by many researchers, creating computed models considering MPS [29,42], brain pressure [1,23] or acceleration thresholds [23]; however, they all fail to respond to the requirement for the extraction of injury severity. This paper will respond to this deficiency and illustrate its potential by studying the case of a 63-year-old male who died from multiple falls. The research will focus on brain white and grey matter trauma injuries.

Methods
The research will revisit a previous study, in which the sequence of events is paraphrased by the authors as: "A 63-year-old male was slightly pushed leading to him taking two steps backward, stop and then fall backward like a stick, and thus sustaining a parieto-occipital head impact on the wooden floor. This event was witnessed by ten people who all gave the same version of the fall. During transportation, the male fell from the stretcher from a height of about 20-30 cm on his right-hand side, sustaining a parietal head impact" [16].
The previous work could capture the location of the trauma, but was unable to compute the injury severity, which this paper will address.
The methodology is illustrated in the flowchart in Figure 7.  Extraction of injury severity has been attempted by many researchers, creating computed models considering MPS [29,42], brain pressure [1,23] or acceleration thresholds [23]; however, they all fail to respond to the requirement for the extraction of injury severity. This paper will respond to this deficiency and illustrate its potential by studying the case of a 63-year-old male who died from multiple falls. The research will focus on brain white and grey matter trauma injuries.

Methods
The research will revisit a previous study, in which the sequence of events is paraphrased by the authors as: "A 63-year-old male was slightly pushed leading to him taking two steps backward, stop and then fall backward like a stick, and thus sustaining a parieto-occipital head impact on the wooden floor. This event was witnessed by ten people who all gave the same version of the fall. During transportation, the male fell from the stretcher from a height of about 20-30 cm on his right-hand side, sustaining a parietal head impact" [16].
The previous work could capture the location of the trauma, but was unable to compute the injury severity, which this paper will address.
The methodology is illustrated in the flowchart in Figure 7. The first step is to age the human-computer model to reflect the body of a 63-year-old. As in 42impact mechanics, stress is a function of the material properties (Equation (7)) [24,41,43], these The first step is to age the human-computer model to reflect the body of a 63-year-old. As in 42impact mechanics, stress is a function of the material properties (Equation (7)) [24,41,43], these values were scaled down from the 39-year-old THUMS 4.01 model to a 63-year-old adult to replicate its frailty due to ageing. σ = Eρ·v where, Equation (7) is a parametric equation used to represent ageing [41]. It is similar to the standard stress wave equation, as it contains some of the parameters used to show ageing, such as Young's Modulus and brain density; it is currently a restriction in ageing models that they only utilise elastic properties.
The 39-year-old THUMS 4.01 white and grey matter material properties were upgraded to include an incompressible Kelvin-Maxwell viscoelastic material behaviours of THUMS 4.02. *MAT61 [26] including the following properties: The human brain is also subject to shrinking with age [44]. Previous work has generated a regression relationship linking brain volume and age [45], which is illustrated in Equation (8). In the model used in this study, the brain white and grey matter were scaled about the brain centre of gravity to adjust for ageing. The grey and white matter were both scaled together with the same ratio.
V age = −0.0037 * age + 1.808 (8) Combining the material degradation and volume changes, it was observed that the computed AIS 4 response curves were reduced when people get older, which is sensible as it takes less power to injure an older person than a younger one. This is illustrated in Figure 8 where a human head was impacted on its forehead with an arbitrary cylindrical object.
its frailty due to ageing.
where,  (7) is a parametric equation used to represent ageing [41]. It is similar to the standard stress wave equation, as it contains some of the parameters used to show ageing, such as Young's Modulus and brain density; it is currently a restriction in ageing models that they only utilise elastic properties.
The 39-year-old THUMS 4.01 white and grey matter material properties were upgraded to include an incompressible Kelvin-Maxwell viscoelastic material behaviours of THUMS 4.02. *MAT61 [26] including the following properties: The human brain is also subject to shrinking with age [44]. Previous work has generated a regression relationship linking brain volume and age [45], which is illustrated in Equation (8). In the model used in this study, the brain white and grey matter were scaled about the brain centre of gravity to adjust for ageing. The grey and white matter were both scaled together with the same ratio.
= −0.0037 * + 1.808 (8) Combining the material degradation and volume changes, it was observed that the computed AIS 4 response curves were reduced when people get older, which is sensible as it takes less power to injure an older person than a younger one. This is illustrated in Figure 8 where a human head was impacted on its forehead with an arbitrary cylindrical object. The AIS 4 equations for the OTM are given in Table 4. The AIS 4 equations for the OTM are given in Table 4. Table 4. AIS interpolation equation [41]. The full OTM models will be presented in the results section. Phase 2 will focus on the accident reconstruction. A THUMS human model, aged to a 63-year-old, is impacted against a stiff wooden structure. A 10-mm-thick wooden structure (300 mm × 300 mm), clamped at its edges (floor is very stiff globally) was modelled with an elastic material property (no cracking). The head was positioned to replicate both impact load cases (Figure 9).  The full OTM models will be presented in the results section. Phase 2 will focus on the accident reconstruction. A THUMS human model, aged to a 63-yearold, is impacted against a stiff wooden structure. A 10-mm-thick wooden structure (300 mm × 300 mm), clamped at its edges (floor is very stiff globally) was modelled with an elastic material property (no cracking). The head was positioned to replicate both impact load cases (Figure 9). The impact scenarios are provided in Table 5. Direction Velocity (m/s) Occipital 6.0 Parietal 1.5

Trauma Calibration Parameter Values (THUMS 4.01 Geometry with 4.02 Brain Material Properties)
In step 3, in the simulation, PVP will be calculated and overlaid over the OTM model. Another important point to notice is that the method used to reconstruct the accidents is using finite elements. As a general principle, finite elements discretise the problem in small elements, which are connected to each other, so the sum of these elements represents the whole problem. By cutting the problem in small parts, it is possible to investigate what can happen locally: this method is used to analyse complex shapes that differ greatly from, for example, simple beams or plates that have been solved by engineers. Usually, organs with a three-dimensional aspect are represented by connected cubes The impact scenarios are provided in Table 5. In step 3, in the simulation, PVP will be calculated and overlaid over the OTM model. Another important point to notice is that the method used to reconstruct the accidents is using finite elements. As a general principle, finite elements discretise the problem in small elements, which are connected to each other, so the sum of these elements represents the whole problem. By cutting the problem in small parts, it is possible to investigate what can happen locally: this method is used to analyse complex shapes that differ greatly from, for example, simple beams or plates that have been solved by engineers. Usually, organs with a three-dimensional aspect are represented by connected cubes (hexahedrons) or triangular-based pyramids (tetrahedrons). This is the case with the computer model used in this study (THUMS 4.01). During the impact, these elements deform, stretch and change shape; however, their volume remains constant. It is called a "Lagrangian" representation of the problem. The consequence, is that, should bleeding occur in the real-world, i.e., loss of volume due to the blood escaping the organ, then the finite elements will not be able to capture this. In order to include the bleeding effect, it is proposed to include the effects of Subdural Hematoma (SDH), which has been defined for an MPS value of 25.5% [46]. The problem then is to assert the AIS outcome from bleeding, as a small bleed could add '1' to the AIS level to the current trauma severity computed or '2' if the bleeding is judged to be important by the pathologist [47]. In some cases, the quantity of blood loss could be subjective, hence for the purpose of being consistent and conservative, all instances of blood loss for the purpose of this study have a '+1' AIS increment on the base AIS computed.

Grey Matter
The PVP value computed was 8.58 mW, which is overlaid over the OTM model for an impact speed of 6.0 m/s. The trauma severity is therefore AIS 3, as illustrated in Figure 10a. However, looking at the MPS values in the grey matter, its value exceeded 25.5% ( Figure 10b); hence, a hemorrhage is present. Consequently, the AIS level is increased by +1, meaning that the final trauma severity computed in the grey matter is AIS 4.

White Matter
The PVP value computed was 5.03 mW, which is overlaid over the OTM model for an impact speed of 6.0 m/s. The trauma severity is, therefore AIS 4, as illustrated in Figure 11a. However, looking at the MPS values in the grey matter, its value exceeded 25.5% (76.9%) ( Figure 11b); hence, a hemorrhage is present. Consequently, the AIS level is increased by +1, meaning that the final trauma severity computed in the grey matter is AIS 5.

Grey Matter
The PVP value computed was 0.05 mW, which is overlaid over the OTM model for an impact speed of 1.5 m/s. The trauma severity is therefore AIS 1, as illustrated in Figure 12a. As the grey matter maximum principal strain did not exceed 25.5% (8.7%), the trauma severity remains unchanged, i.e., AIS 1 (Figure 12b).

White Matter
The PVP value computed was 0.07 mW, which is overlaid over the OTM model for an impact speed of 1.5 m/s. The trauma severity is therefore AIS 1, as illustrated in Figure 13a. As the grey matter maximum principal strain did not exceed 25.5% (8.4%), the trauma severity remains unchanged, i.e., AIS 1 (Figure 13b).

Discussion
The results are summarized in Table 6 and are compared with the Post-Mortem (PM). It can be noted that the trauma severity correctly matches the PM. In all the cases, the head injury predictions had some similarities with the post-mortem results, as shown in Table 6. When no evidence was recorded in the PM, it did not necessarily mean that there was no injury, but that there was no observable injury. Consequently, no observation could mean that the AIS range could be from 0 to 2. This step has been taken, as it was found that overall, the quality of autopsy reports (PM) is often questioned: just half of PM reports 52% (873/1691) were considered satisfactory by experts, 19% (315/1691) were good and only 4% (67/1691) were excellent. Over a quarter were marked as poor or unacceptable. Proportionately, there were more reports rated 'unacceptable' for those cases that were performed in a local authority mortuary (21/214 for local authority mortuary cases versus 42/1477 for hospital mortuary cases)" [40]. Consequently, for trauma injury severity cases not observed in the PM, a probable PM range has been included in the results of OTM models.
The location of the trauma severity is plotted in Figures 14 and 15. In the computer model, there is evidence of trauma near the contact area between the head and the wooden block. The computer model suggests the same location as the CT scan.  In the computer model, there is evidence of trauma near the contact area between the head and the wooden block. The computer model suggests the same location as the CT scan. The computer model also suggests trauma on the other side of the brain, as per the CT scan.
In the CT scan information provided in the paper [1], the location of the slides was not provided; hence, it is not possible to categorically assert whether the comparisons are exact. Nevertheless, the trauma pattern from Figure 16 is consistent with what has been reported. In the computer model, there is evidence of trauma near the contact area between the head and the wooden block. The computer model suggests the same location as the CT scan.
The computer model also suggests trauma on the other side of the brain, as per the CT scan.
In the CT scan information provided in the paper [1], the location of the slides was not provided; hence, it is not possible to categorically assert whether the comparisons are exact. Nevertheless, the trauma pattern from Figure 16 is consistent with what has been reported.

Conclusions
This paper has presented an organ trauma model (OTM), based on the second law of thermodynamics, which has been applied to modelling brain white and grey matter injuries. This trauma model was used to model the fatal fall of a 63-year-old male. As the computer model uses constant volume Lagrangian finite element modelling, an additional criterion was added to consider the hemorrhaging aspect of the trauma. This was achieved by monitoring the maximum principle strain of the brain and factoring 1 AIS if the threshold on 25.5% was computed, concurring with physiological soft tissue tests.
The predictions correctly matched the post-mortem in injury severity. Overall, it was also observed that the location of the trauma was comparable to the post-mortem CT scans; however, as the location of the slice was not provided, it is not possible to categorically confirm this.

Conclusions
This paper has presented an organ trauma model (OTM), based on the second law of thermodynamics, which has been applied to modelling brain white and grey matter injuries. This trauma model was used to model the fatal fall of a 63-year-old male. As the computer model uses constant volume Lagrangian finite element modelling, an additional criterion was added to consider the hemorrhaging aspect of the trauma. This was achieved by monitoring the maximum principle strain of the brain and factoring 1 AIS if the threshold on 25.5% was computed, concurring with physiological soft tissue tests.
The predictions correctly matched the post-mortem in injury severity. Overall, it was also observed that the location of the trauma was comparable to the post-mortem CT scans; however, as the location of the slice was not provided, it is not possible to categorically confirm this.
This study has been validated using one accident case, and it is necessary to extend this validation with more cases (fall), considering different scenarios like pedestrian accident cases for example, where head injuries are the biggest cause of death.
This model provides however important advances in brain trauma modelling, and delivers a tool that could be used in forensic investigation, pending a correlation with a larger sample of samples of fall cases. In order to refine the results, future work could be consider researching MPS AIS 4 threshold levels and ageing, so that the OTM model could be further refined.