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Separations, Volume 7, Issue 4

December 2020 - 26 articles

Cover Story: Nanoliquid chromatography has enabled the enantiomeric separation of two chiral drugs, lacosamide and colchicine, in less than 8 and 9 min with enantiomeric resolutions of 1.6 and 2.3, respectively. A capillary column lab-packed with an amylose tris (3,5-dimethylphenylcarbamate) chiral stationary phase was used. This methodology is characterized by the low volume of mobile phase and sample required (1.8 µL per analysis and 20 nL sample). The proposed method can detect the enantiomeric impurities of both drugs up to a 0.1% with respect to the majority enantiomer and can successfully be applied to ensure quality control of these drugs in pharmaceutical formulations. View this paper.
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Articles (26)

  • Article
  • Open Access
2 Citations
4,051 Views
13 Pages

The Influence of Mycorrhizal Fungi on the Accumulation of Sennosides A and B in Senna alexandrina and Senna italica

  • Mashail N. AlZain,
  • Abdulrahman A. AlAtar,
  • Abdulaziz A. Alqarawi,
  • Ramzi A. Mothana,
  • Omar M. Noman,
  • Rashed N. Herqash,
  • Ebtesam S. AlSheddi,
  • Nida N. Farshori and
  • Perwez Alam

Symbiotic arbuscular mycorrhizal fungi (AMF) play a major role in plant development, growth, and relationships with the environment through a change in the accumulation of secondary metabolites; hence, we planned to investigate AMF’s influence...

  • Article
  • Open Access
3 Citations
3,706 Views
18 Pages

Screening Carpet Substrate Interferences in Arson Identification by Solid Phase Microextraction and Gas Chromatography-Mass Spectrometry

  • Alyssa Aldrich,
  • Edna Gennarino-Lopez,
  • Gabriel Odugbesi,
  • Kaylandra Woodside and
  • Shokouh Haddadi

The sample analysis and data interpretation is the most challenging step of fire debris analysis, due to the presence of combustion and pyrolysis products in the substrate material. In this study, a headspace solid phase microextraction (HS-SPME) pro...

  • Article
  • Open Access
3 Citations
3,518 Views
12 Pages

Development of a rapid approach for universal microbial detection is required in the healthcare, food and environmental sectors to aid with medical intervention, food safety and environmental protection. This research investigates the use of enzymati...

  • Communication
  • Open Access
1 Citations
4,219 Views
9 Pages

An ultra-performance liquid chromatography-tandem mass spectrometry method was developed for the quantification of clotrimazole (CTZ) plasma levels after intravaginal administration of the drug given at approved dosages. Plasma samples were extracted...

  • Article
  • Open Access
2,919 Views
11 Pages

The quantitative analysis of the chromatographic behavior of basic compounds measured with pentyl-, hexenyl-, and octyl-bonded silica gels were analyzed in silico employing model phases. The main retention force was the van der Waals (VW) interaction...

  • Article
  • Open Access
36 Citations
7,829 Views
20 Pages

Development and Validation of Rapid RP-HPLC and Green Second-Derivative UV Spectroscopic Methods for Simultaneous Quantification of Metformin and Remogliflozin in Formulation Using Experimental Design

  • Mahesh Attimarad,
  • Rafea Elamin Elgack Elgorashe,
  • Rajasekaran Subramaniam,
  • Mohammed Monirul Islam,
  • Katharigatta N. Venugopala,
  • Sreeharsha Nagaraja and
  • Abdulmalek Ahmed Balgoname

Recently, a new formulation containing metformin HCl (MFH) and remogliflozin etabonate (RGE) has been approved for the management of diabetes mellitus. However, only one analytical method has been reported for the simultaneous determination of both t...

  • Article
  • Open Access
15 Citations
6,267 Views
10 Pages

Currently, the medical use of food supplements containing Cannabis sativa has attracted the interest of consumers, as well as the medical and scientific community. With the increasing consumption of these products, there is also a risk of their abuse...

  • Article
  • Open Access
4 Citations
4,408 Views
16 Pages

Improved Renoprotection in Diabetes with Combination Therapy of Coccinia indica Leaf Extract and Low-Dose Pioglitazone

  • Girish Meravanige Basavarajappa,
  • Prem Kumar Nanjundan,
  • Abdulrahim Alabdulsalam,
  • Afzal Haq Asif,
  • Hema Tyavanige Shekharappa,
  • Md. Khalid Anwer and
  • Sreeharsha Nagaraja

Background: The metabolic changes associated with diabetes can lead to nephropathy eventually resulting in end-stage renal disease. Current antidiabetic therapies do not effectively prevent the onset of diabetic kidney diseases as well as progression...

  • Feature Paper
  • Article
  • Open Access
4,186 Views
11 Pages

Globotriaosylsphingosine (LysoGb3) is a biomarker for Fabry disease (OMIM 301500) that contains long-chain bases (LCBs) as a building block. There have been several studies proposing that LysoGb3 forms with distinct LCBs could be putative disease sub...

  • Article
  • Open Access
23 Citations
10,372 Views
16 Pages

Biological Activity of Cannabis sativa L. Extracts Critically Depends on Solvent Polarity and Decarboxylation

  • Guillermo Moreno-Sanz,
  • Carlos Ferreiro Vera,
  • Carolina Sánchez-Carnerero,
  • Xavier Nadal Roura and
  • Verónica Sánchez de Medina Baena

Minor cannabinoid and non-cannabinoid molecules have been proposed to significantly contribute to the pharmacological profile of cannabis extracts. Phytoplant Research has developed highly productive cannabis cultivars with defined chemotypes, as wel...

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Separations - ISSN 2297-8739