Isolation of Arborescin from Artemisia absinthium L. and Study of Its Antioxidant and Antimicrobial Potential by Use of In Vitro and In Silico Approaches

: This study focused on developing an innovative, straightforward, and economical method utilizing a mixture of readily available solvents to extract arborescin (C 2 OH 2 OO 8 ) crystals from Artemisia absinthium L. ( A. absinthium ). The structural elucidation and characterization were conducted using a suite of techniques including IR spectroscopy, CNHSO elemental analysis, scanning electron microscopy and energy dispersive X-ray spectroscopy (SEM-EDS), and mass spectroscopy (MS). Density functional theory (DFT) calculations were employed to determine the molecular properties. Antioxidant activity was measured using the DPPH radical scavenging assay and the β -carotene bleaching test. Antimicrobial efficacy was assessed against four bacterial strains and three fungal strains. The molecular docking approach was employed to predict the probable binding patterns and affinities of arborescin with specific target biomolecules. Employing an array of analytical techniques, examination of the isolated crystal from A. absinthium . led to its comprehensive structural elucidation. IR spectroscopy revealed the presence of distinctive functional groups, including a carbonyl group within the γ -lactone and an epoxy group. CNHSO elemental analysis verified that the crystal contained only carbon, hydrogen, and oxygen, a finding corroborated by SEM-EDS analysis, consistent with the molecular structure of arborescin. Additionally, mass spectrometry confirmed the identity of the compound as arborescin, with a molecular ion with a mass m / z = 248. Quantum-Chemical Descriptors revealed that arborescin is resistant to elementary decomposition under standard conditions. Although arborescin demonstrates a relatively low antioxidant capacity, with an IC50 of 5.04 ± 0.12 mg/mL in the DPPH assay, its antioxidant activity in the β -carotene bleaching test was


Introduction
Plants are an invaluable source of bioactive molecules, containing a variety of compounds with beneficial properties for health.These natural substances offer valuable therapeutic potential, making plants an essential resource in medical research and pharmacology [1].As per the World Health Organization (WHO), a significant portion, approximately 80%, of the global populace relies on plants and herbs for medicinal purposes [2].Moreover, plants offer a distinct advantage in the realm of drug discovery, attributed to their extensive historical utilization by humans.This extended usage suggests a potential for decreased toxicity in plant-derived medications, alongside an abundance of bioactive compounds [3].Also, extracts or compounds from plants offer an alternative source of antimicrobials and antioxidants that is natural, more reliable, inexpensive, and time-tested compared to other antibiotics; also, they rarely have severe side effects [4].Investigating the antioxidant, antibacterial, and antifungal properties of molecules isolated from plants offers promising potential for the discovery of new drugs.By isolating molecules, we can obtain a large number of molecules that can be used as active components in several fields, including agri-food, biotechnology, cosmetics, and pharmacology.A. absinthium holds significance as a perennial shrubby plant extensively employed in addressing various health conditions.
A. absinthium L., belonging to the Asteraceae family, is a perennial plant with yellow flowers that widely grows in sunny and dry regions of Europe, Siberia, South America, North America, and North Africa [5]. A. absinthium goes by several vernacular names.In English, it is recognized as absinthe, green ginger, wormwood, and absinthium.The French refer to it as Vermouth, and in Latin it is identified as Genepi.The Greek name is Apsinthion, and in Chinese it is known as Yang ai and Kuai [6].In Hindi, it is known as Mastiyarah, Majtari, Karmala, or Majri.The Japanese term is nigayomogi, while in German it is recognized as Absinth or Wermut.In Arabic, it goes by names Afsanteen or Damseeh [7].
The plant's phytochemical examination has indicated the existence of chemical constituents, including polyphenolic compounds [8] and flavonoids [9] as well as fatty acids [10].Also, the analysis suggests that the plant predominantly contains terpenoids, coumarins, caffeoylquinic acids, sterols, acetylenes, tannins, carotenoid glucosides, and sesquiterpene lactones or bitter principles [11][12][13][14].Sesquiterpenic lactones or bitter principles, characteristic of the Asteraceae family, exhibit properties such as antitumor, cytotoxic, antifungal, and antibacterial activities [14].The stimulant effects of A. absinthium rely on the existence of bitter compounds like artabsine and absinthine, both of which are sesquiterpene lactones found in the plant's extracts [15].Sesquiterpene lactones are a stable, bitter, and colorless subfamily of terpenoids, which are a class of lipophilic plant secondary metabolites, and they are almost exclusively derived from the Asteraceae family (Compositae) [16].With over 11,000 sesquiterpenes recorded in the Dictionary of Natural Products, Sesquiterpene lactones make up a significant and biogenetically homogeneous group of natural compounds.Close to 5000 of these sesquiterpenes contain at least one lactone group; given their broad spectrum of biological activities, they act as an effective defense mechanism for the organisms that synthesize them [17].It has been demonstrated that this category of molecules is correlated with activities such as inhibiting tumor growth, inducing cell death, phytotoxic activity, and antimicrobial activity [18].The diverse range of activities exhibited by sesquiterpene lactones implies that these natural compounds have evolutionary importance in plants, serving as deterrents against herbivores and exhibiting antibacterial and antifungal properties, as well as acting as allelopathic agents [14].
Absinthin, a dimeric sesquiterpene lactone, has been extracted from the plant A. absinthium using HPLC-solid phase extraction [19].Two diastereometric homoditerpeneperoxides have been isolated from A. absinthium and the combination of these compounds demonstrated in vivo antimalarial activity, exhibiting an IC50 of 1 µg/mL [20].Another study identified sofalcone glycosides in the methanolic extract of the plant's aerial parts, characterized as artemisia isoflavonyl glucosyl diester and artemisia bis-isoflavonyl dirhamnoside [21].Artemetin is a flavonoid isolated from leaves of A. absinthium [22]; it demonstrated hepatoprotective effects [23] as well as antimalaria activity [24].Moreover, it exhibited anti-inflammatory and antioxidant actions [25] along with anticancer properties [26].Being an unstable compound, it easily transforms into anabsinthin, and this molecule has exhibited anti-inflammatory activity in human bronchoepithelial cells [27].Anabsin diguaianolide was isolated from A. absinthium L. [28].Arborescin was isolated from Artemisia gorgonum, exhibiting a significantly lower IC50 than the antitumoral drug etoposide, demonstrates notable cytotoxic activity in vitro, especially against tumoral cells, and is less toxic to nontumoral cells, suggesting its potential as a target for further studies to unravel the underlying mechanisms of its cytotoxic effects [29].It was also isolated from flower baskets and leaves of Artemisia austriaca Jacq [30].Crystalline arborescin was obtained from Artemisia adamsii [31].
The bibliography gives a very limited number of works and almost no interest has been devoted to thermal characteristics and biological activities.For this, in this study, our focus is on structural identification, employing a combination of techniques, such as elemental analysis, infrared and mass spectroscopy, and scanning electron microscopy.A theoretical investigation was carried out using density function theory to ascertain the parameters associated with this molecule.Exploring its potential applications in pharmacology, we proceeded to assess the molecule's activity as an antioxidant, antibacterial, and antifungal agent, and the obtained results were correlated with docking analyses.
The current challenges in treating infections caused by bacteria or fungi remain crucial, as it is often difficult to develop highly effective drugs with minimal adverse effects.Therefore, the discovery of medications derived from nature, particularly plant secondary metabolites, remains an attractive alternative for researchers and scientists.The molecule arborescin is under-studied, and its properties are poorly understood.Thus, our objective is twofold: first, to valorize this molecule isolated from a widely available plant; and second, to search for a new antimicrobial agent capable of competing in terms of cost and effectiveness with the antimicrobials already known.

Plant Material
The A. absinthium L. used in this study was first gathered in March 2021 from Zkara, a rural community in Mestferki, Morocco.This location is situated in the Oujda Angad prefecture, in the Oriental region of Morocco (Figure 1).The fresh leaves of the plant were harvested and dried in a dark, dry place at room temperature until all moisture was removed.They were then stored in a cool environment (4 • C) for later use.Just before use, the dried plant was reduced to a powder.

Isolation Processus
The extraction of 50 g of A. absinthium powder involved using a 200 mL water/acetone mixture (3/7) and heating it at reflux for 2 h at a temperature of 65-70 °C.The resulting extract underwent concentration via vacuum evaporation until approximately one-third of the solvent was eliminated, after which it was extracted with 50 mL of hexane.The hexane fraction was retrieved and subjected to slow evaporation of the solvent in a dry location at a temperature between 10 and 15 °C.Transparent crystals began to form and grow during this process, and they were collected via vacuum filtration.A basic recrystallization procedure was then performed to obtain purified crystals.Initially, the impure crystals were dissolved in minimum of acetone, and then cold methanol was added.This process was repeated until a very pure crystal was obtained, confirmed by a single spot on thin-layer chromatography.The isolated crystals, weighing 11 mg, were stored in glass vials at a temperature of 4 °C.

High-Performance Liquid Chromatography
The purity analysis of the crystalline phase was conducted utilizing a liquid chromatography separation module (Waters e2695) in conjunction with a diode array detector (Waters 2998 PDA) from Waters Corporation company, united states.This detector records the ultraviolet and visible absorption spectra of the samples, providing qualitative information.The detector is integrated into the Empower data processing software.Analyses followed the protocol described by Hbika et al. [32].Chromatograms were recorded between 254 and 300 nm using a C18 column (4.6 × 250 mm, 5 µm).The mobile phase, in gradient mode, consisted of ultrapure water/acetic acid (0.5%) (solvent A) and methanol (solvent B) with the following proportions: 0 min, 80% A and 20% B; 20

Isolation Processus
The extraction of 50 g of A. absinthium powder involved using a 200 mL water/acetone mixture (3/7) and heating it at reflux for 2 h at a temperature of 65-70 • C. The resulting extract underwent concentration via vacuum evaporation until approximately one-third of the solvent was eliminated, after which it was extracted with 50 mL of hexane.The hexane fraction was retrieved and subjected to slow evaporation of the solvent in a dry location at a temperature between 10 and 15 • C. Transparent crystals began to form and grow during this process, and they were collected via vacuum filtration.A basic recrystallization procedure was then performed to obtain purified crystals.Initially, the impure crystals were dissolved in minimum of acetone, and then cold methanol was added.This process was repeated until a very pure crystal was obtained, confirmed by a single spot on thin-layer chromatography.The isolated crystals, weighing 11 mg, were stored in glass vials at a temperature of 4 • C.

High-Performance Liquid Chromatography
The purity analysis of the crystalline phase was conducted utilizing a liquid chromatography separation module (Waters e2695) in conjunction with a diode array detector (Waters 2998 PDA) from Waters Corporation company, united states.This detector records the ultraviolet and visible absorption spectra of the samples, providing qualitative information.The detector is integrated into the Empower data processing software.Analyses followed the protocol described by Hbika et al. [32].Chromatograms were recorded between 254 and 300 nm using a C18 column (4.6 × 250 mm, 5 µm).The mobile phase, in gradient mode, consisted of ultrapure water/acetic acid (0.5%) (solvent A) and methanol (solvent B) with the following proportions: 0 min, 80% A and 20% B; 20 min, 0% A and 100% B; 25 min, 0% A and 100% B. The flow rate was 1 mL/min, and the injection volume was 20 µL.
Peaks were identified by comparing retention times and UV spectra with those of standards.The standard polyphenolic compounds used included gallic acid, vanillic acid, catechin, caffeic acid, salicylic acid, p-hydroxybenzoic acid, rutin, quercetin, syringic acid, vanillin, naringenin, p-coumaric acid, artemetin, ascorbic acid, ferulic acid, malic acid, and kaempferol, which have been previously found in A. absinthium.

Elemental Analysis
The elemental analysis conducted on the CNHSO involved determining the relative proportions of carbon (C), sulfur (S), hydrogen (H), nitrogen (N), and oxygen (O) in the compound.This was achieved using the EuroEA 3000, which is a specialized instrument designed to analyze the elemental composition of organic compounds.The sample was carefully prepared and then subjected to high-temperature combustion, which converted the organic material into a gas that could be analyzed using the instrument.Instantaneous and complete oxidation of the sample to be analyzed is achieved by combustion with oxygen at a temperature of 1060 • C, with the furnace temperature set to 100 • C. The combustion products are then carried by the gas carrier to a column where separation occurs.The carrier pressure is 110 KPa.A thermal conductivity sensor provides the signal for each element, which is then translated into a percentage content.The operation time is 320 s and the sampling time is 5 s.

Fourier Transform Infrared Spectroscopy FT-IR
Fourier-transform infrared spectroscopy (FT-IR) analysis in the solid phase is carried out using an FTIR-4700 JASCO instrument, with recordings conducted within the 4000-400 cm −1 region.An interferogram is then recorded, composed of the detector signal as a function of time, and the resulting absorption spectrum is obtained by Fourier transformation of the interferogram [33].

Mass Spectroscopy
The technique of mass spectrometry (MS) is a critical physicochemical analytical tool for qualitative chemical analysis.To measure the exact masses of the atoms in the samples, a direct injection was performed using a Shimadzu GCMS-QP2010 instrument in electron impact mode.
This methodology entails the separation of charged molecules (ions) in the gas phase according to their mass-to-charge ratio (m/z).The function of a mass analyzer is to measure the mass-to-charge ratios (m/z) of ions.Its operating principles rely on the interaction of charged particles with electric or magnetic fields [34].The mass spectrometer is comprised of an ionization chamber, an analyzer for separating the ions, and an ion detector.The resulting data allowed for the identification and quantification of the individual atoms present in the samples.

MEB/EDX
For the examination of both morphology and atomic composition, scanning electron microscopy (SEM-EDX) was employed, using a JEOL JSM-IT500HR model instrument.The crystal was positioned on a carbon strip affixed to a sample holder and subjected to observation under an operating voltage of 10 kV.This method facilitated a thorough investigation of the surface structure of the crystal and the elements comprising it.The combination of Energy-Dispersive X-ray Spectroscopy (EDX) and the Scanning Electron Microscope (SEM) enables high-resolution imaging and elemental analysis of samples.

Density Function Theory Study
The entire optimization process for the geometry of the isolated molecule was conducted utilizing density functional theory (DFT), employing the B3LYP hybrid functional [32].Calculations were executed at the DFT/B3LYP level of theory using the 6-311 G (d,p) basis set integrated into Gaussian 09 W software, renowned for its sophisticated features in modeling electronic structures.These computations facilitated the determination of parameters characterizing the molecule's overall reactivity, such as total energy (ET), highest occupied molecular orbital energy (EHOMO), lowest unoccupied molecular orbital energy (ELUMO), energy gap (∆Egap), dipole moment (µ), electron affinity (Af), ionization potential (I), electronegativity (χ), global softness (σ), global hardness (η), electrophilicity (ω), and chemical potential (µ) [22].
2.9.Antioxidant Activity 2.9.1.Scavenging 2,2-Diphenyl-1-picrylhydrazyl Radical Test The antioxidant potential of arborescin was evaluated through the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical decolorization assay [35,36], a well-established method for assessing the antioxidative properties of natural substances by measuring their capability to neutralize free radicals.In this assay, varying concentrations of arborescin solution (ranging from 0.0625 to 2 mg/mL) were combined with freshly prepared methanolic DPPH solution (0.1 mM) in a ratio of 500 µL to 3 mL, respectively.To establish a negative control, 500 µL of the solvent utilized for extract solubilization was mixed with a 3 mL solution of DPPH prepared in methanol.Subsequently, the absorbance of the decolorized DPPH solution was determined against a blank solution at a wavelength of 517 nm following a 20 min incubation period at room temperature and in darkness, utilizing a UV/visible spectrophotometer.Ascorbic acid served as the positive control, and its absorbance was measured under identical conditions and procedures as those employed for the sample.Each concentration was subjected to triplicate measurements.The scavenging activity was quantified using the formula provided below: where A0 represents the absorbance of the control reaction, and A1 represents the absorbance of the test sample.

β-Carotene Bleaching Test
The carotene bleaching test was used to evaluate the antioxidant capacity of arborescin, following the procedure described by Kartal et al. [37,38].The β-carotene/linoleic acid emulsion was prepared by dissolving 2 mg of β-carotene in 1 mL of chloroform, along with 25 µL of linoleic acid and 200 mg of Tween 80.The chloroform was then evaporated using a rotary evaporator.Subsequently, 100 mL of distilled water saturated with 30% H 2 O 2 was added to the dried mixture, and the resulting emulsion was thoroughly stirred to ensure uniformity.Following this, 175 µL of the test molecule solution or a solution of butylated hydroxyanisole (BHA) at a concentration of 2 mg/mL was added to 1.25 mL of the emulsion.The decolorization kinetics of the negative control emulsion, arborescin, and the BHA were monitored at 490 nm over 120 min at regular intervals.The relative antioxidant activity (RAA) of the molecule was determined using the formula below: where A120 represents the absorbance values of the solution containing arborescin after 120 min of incubation, and C0 and C120 represent the absorbance values of the control before and after 120 min of incubation, respectively.

Bacterial strains
In this investigation, we employed four bacterial strains: Escherichia coli (ATCC 10536), Staphylococcus aureus (ATCC 6538), Listeria innocua (ATCC 33090), and Pseudomonas aeruginosa (ATCC 15442), serving as representative microorganisms to assess the antibacterial effectiveness of the four synthesized molecules.These strains were sourced from the Laboratory of Bioresources, Biotechnology, Ethnopharmacology, and Health, situated within the Faculty of Sciences at Oujda, Morocco [39].

Fungal strains
For this investigation, three distinct fungal strains, Aspergillus niger, Candida glabrata, and Penicillium digitatum, were chosen to evaluate the antifungal capabilities of the four molecules under scrutiny.These fungal strains were sourced from the Laboratory of Bioresources, Biotechnology, Ethnopharmacology, and Health, housed within the Faculty of Science at Oujda, Morocco.

3.
Determination of MIC, MBC, and MFC Determining the minimum inhibitory concentration (MIC) is a critical step in assessing the efficacy of antimicrobial agents.The determination of the minimum inhibitory concentration (MIC) for the four molecules under investigation utilized the resazurin microtitration assay.This assay was conducted in 96-well microplates, with each well containing concentrations ranging from 15 mg/mL to 0.05 mg/mL of the test compounds.A standardized inoculum of the respective microbial strain was introduced into each well, followed by incubation at the appropriate temperature: 37 • C for bacterial strains and 25 • C for fungal strains, both for a duration of 18 h.Following incubation, resazurin was introduced into the microplates, and a subsequent 2 h incubation period ensued until a color change from blue to pink was evident [40].
MBC and MFC were determined by inoculating a 3 µL sample taken from negative wells onto agar media plates, which were then incubated at the appropriate temperature for each type of microbial strain for 24 h.The lowest concentration of samples that did not result in microbial growth was used to establish MBC and MFC [39].

Molecular Docking Protocol
The utilization of the molecular docking methodology facilitated the anticipation of plausible binding configurations and affinities between isolated plant metabolites and specific target biomolecules [41].Employing a computational docking strategy, we projected the potential antioxidant and antibacterial properties of arborescin, adhering to the methodologies outlined in prior studies [41,42].In accordance with established literature, we carefully selected distinct proteins for interaction: xanthine oxidoreductase (PDB ID: 3AX7) for antioxidant evaluation [43], and dihydrofolate reductase (DHFR) enzyme (PDB ID: 4M6J) for assessing antibacterial effects [44].Acquisition of the three-dimensional crystal structures of these designated proteins was accomplished from the RCSB Protein Data Bank (source: https://www.rcsb.org;accessed on 1 February 2024) in PDB format.

1.
Subsequent to this step, we employed the PyMoL 2.3 tool to preprocess all macromolecules, eliminating water molecules and extraneous protein residues.In order to guarantee structural integrity, nonpolar hydrogen atoms were integrated into the refined proteins.Sequentially, the Swiss PDB viewer, renowned for its energy minimization capabilities [45], was employed to minimize their energy states to the lowest possible level.After these purification and optimization processes, the macromolecules were archived in PDB format and primed for subsequent analysis.

2.
Throughout the computational interaction process, we implemented a semi-flexible modeling approach utilizing the extensively employed PyRx AutoDock Vina molecular docking software.Within PyRx, the target proteins were prepared and designated as macromolecules [46].The 3D conformers of all ligands, initially in SDF format, were introduced into PyRx and subjected to energetic optimization.Subsequently, they were converted to pdbqt format using the Open Babel tool within the PyRx AutoDock Vina software, with a selection of the most optimal hit [47].The results of the docking analysis were projected, and the outcomes, in conjunction with the docked macromolecules and ligands, were exported as output files in pdbqt format.These ligand and macromolecule files were merged and preserved in PDB format for further scrutiny using PyMol software.Ultimately, 2D visualizations were generated utilizing Discovery Studio Visualizer (version 4.6).

High-Performance Liquid Chromatography Analysis
The process of isolating a molecule in the liquid phase often involves the coexistence of various other molecules, even at low concentrations.In order to purify the solid phase, we adopted a sequential process of dissolution and recrystallization to minimize the presence of parasitic molecules, and the verification was conducted using thin layer chromatography until a spot was obtained.High-performance liquid chromatography (HPLC) characterization of the resulting crystal did not show any peaks; this suggests that this molecule does not fall within the range of compounds that absorb wavelengths between 254 and 300 nm.The diode array detector used is specifically designed for the identification of elements with wavelengths generally ranging from 190 to 400 nm (ultraviolet).
The lack of results for chromatographic analysis, attributed to the absence of UV absorbance, may be explained by the presence of a molecule lacking a distinct chromophore and featuring a peroxy bridge.Indeed, although high-performance liquid chromatography with ultraviolet detection is widely available, it has not been used for the analysis of certain sesquiterpene lactones, such as artemisinin, due to the reported lack of UV absorption for this lactone [48].Determining artesunate is also challenging, similar to artemisinin, due to the absence of a distinct chromophore and the presence of a peroxy bridge that absorbs at higher wavelengths [49][50][51].Additionally, absorbance responses increase with the presence of double bonds.The detection limit of HPLC-DAD is also not low enough, rendering this method incapable of detecting dihydroartemisinin, a sesquiterpene lactone [48].

Infrared Spectroscopy
The spectral range spanning from 1000 to 4000 cm −1 , often referred to as the functional groups region, is paramount in furnishing the bulk of information utilized for the interpretation of an IR spectrum.Conversely, the segment spanning between 400 and 1000 cm −1 , recognized as the fingerprint region, encompasses a multitude of peaks and tends to exhibit greater complexity, rendering the assignment of individual peaks more challenging.
The IR spectrum of the compound CR1 presented in Figure 2

Scanning Electron Microscopy and Energy-Dispersive Spectroscopy
Scanning electron microscope observations of the solid crystal have revealed a crystalline morphology characterized by square layers that have sedimented onto each other.The clearly defined lines observed vividly illustrate the sedimentation process, where these square layers overlap in an organized manner (Figure 3).

Scanning Electron Microscopy and Energy-Dispersive Spectroscopy
Scanning electron microscope observations of the solid crystal have revealed a crystalline morphology characterized by square layers that have sedimented onto each other.The clearly defined lines observed vividly illustrate the sedimentation process, where these square layers overlap in an organized manner (Figure 3).

Scanning Electron Microscopy and Energy-Dispersive Spectroscopy
Scanning electron microscope observations of the solid crystal have revealed a crystalline morphology characterized by square layers that have sedimented onto each other.The clearly defined lines observed vividly illustrate the sedimentation process, where these square layers overlap in an organized manner (Figure 3).The results of the energy dispersive X-ray spectroscopy (EDS) analysis conducted on the crystal's chemical composition distinctly indicate that the predominant atoms of the compound are carbon and oxygen, as depicted in Table 1.The obtained molar ratio of C/O is 4.93, highlighting the relative proportion of these elements in the material's composition.The results of the energy dispersive X-ray spectroscopy (EDS) analysis conducted on the crystal's chemical composition distinctly indicate that the predominant atoms of the compound are carbon and oxygen, as depicted in Table 1.The obtained molar ratio of C/O is 4.93, highlighting the relative proportion of these elements in the material's composition.

Mass Spectrometer
The mass spectrometer (MS) of the compound obtained in electron impact mode reveals a molecular ion with a mass m/z = 248 (Figure 4), suggesting a molar mass of 248 g/mol.This mass corresponds to that of arborescin with the formula C 15 H 20 O 3 (Figure 5).The various possible fragments are shown in Figure 6.The MS spectrum provides the following ions: Separations 2024, 11, x FOR PEER REVIEW 10 of 22

Mass Spectrometer
The mass spectrometer (MS) of the compound obtained in electron impact mode reveals a molecular ion with a mass m/z = 248 (Figure 4), suggesting a molar mass of 248 g/mol.This mass corresponds to that of arborescin with the formula C15H20O3 (Figure 5).The various possible fragments are shown in Figure 6

Elemental Analysis CNHSO
The elemental analysis of CNHSO, conducted on the solid phase, yielded weight ratios of C/O close to those of the empirical formula of arborescin, as shown in Table 2.The results demonstrate that the isolated molecule consisted solely of carbon, oxygen, and hydrogen atoms.The experimental and theoretical ratios obtained were 9.29 and 9.00, respectively.This slight difference could be attributed either to instrument error or the potential presence of impurities in the isolated molecule.An ion at m/z 215, indicative of a loss of 44 u from m/z 233, suggests the loss of CHO 2 3• These findings offer invaluable insights into the molecular structure and fragmentation patterns of the compound, enabling a comprehensive characterization of its molecular composition.

Elemental Analysis CNHSO
The elemental analysis of CNHSO, conducted on the solid phase, yielded weight ratios of C/O close to those of the empirical formula of arborescin, as shown in Table 2.The results demonstrate that the isolated molecule consisted solely of carbon, oxygen, and hydrogen atoms.The experimental and theoretical ratios obtained were 9.29 and 9.00, respectively.This slight difference could be attributed either to instrument error or the potential presence of impurities in the isolated molecule.

Quantum Chemistry Calculations
In Figure 7, the refined arrangement of arborescin is illustrated alongside the threedimensional depictions of its frontier orbitals, namely the Highest Occupied Molecular Orbital (HOMO) and the Lowest Unoccupied Molecular Orbital (LUMO), as observed in its fundamental state.Within these depictions, the HOMO and LUMO orbitals exhibit discernible phases denoted by red and green, respectively, highlighting positive and negative charge distributions.A thorough examination of the three-dimensional representations of arborescin's frontier orbitals reveals a notable concentration of electron charges predominantly localized on the pentane cycle and the CH 3 group attached to it.
Table 3 presents the computed energies and various parameters utilizing the B3LYP 6-311G (d,p) method.The energy associated with the Highest Occupied Molecular Orbital (HOMO) directly correlates with the ionization potential, whereas the energy corresponding to the Lowest Unoccupied Molecular Orbital (LUMO) is directly associated with the electron affinity.These computations offer valuable insights into the electronic and reactive characteristics of arborescin, contributing to a comprehensive comprehension of its molecular chemistry.Analysis of the results indicates that a significant energy gap (∆Egap) signifies heightened molecular stability and reduced aromaticity, whereas a minimal disparity between HOMO and LUMO suggests the system's flexibility and high polarizability [52,53].Arborescin exhibits negative values for both EHOMO and ELUMO energy levels, indicating its stability.With a low energy gap (∆Egap) of 6.73 eV, the molecule demonstrates high chemical activity, categorizing it as "soft".Such molecules possess a higher reactivity compared to their "hard" counterparts.Additionally, the global hardness obtained is low, at 3.36 eV.The dipole moment offers insights into the polarity of the entire molecule [54].
Electrophilicity pertains to an electrophile's capacity to gain an extra electron charge and its resilience to exchanging electron charges with the environment [55].The negative chemical potential (−3.41 eV) indicates the molecule's resistance to decomposition into elements.Accordingly, a substantial dipole moment of 5.47 D is evident, indicating pronounced molecular polarity, likely augmenting its high chemical reactivity.Table 3 presents the computed energies and various parameters utilizing the B3LYP 6-311G (d,p) method.The energy associated with the Highest Occupied Molecular Orbital (HOMO) directly correlates with the ionization potential, whereas the energy corresponding to the Lowest Unoccupied Molecular Orbital (LUMO) is directly associated

DPPH Radical Trapping Test
Figure 8 illustrates the antioxidant powers of arborescin.Overall, the tested compound exhibited a low DPPH consumption, with inhibition percentages not exceeding 20% for a concentration of 2 mg/mL.The IC50 test values are provided in Table 4. Arborescin demonstrates modest antioxidant activity, evidenced by an IC50 value of 5.04 ± 0.12, significantly distant from that of ascorbic acid, which gives an IC50 value of 0.16 ± 0.003 mg/mL.8 illustrates the antioxidant powers of arborescin.Overall, the tested compound exhibited a low DPPH consumption, with inhibition percentages not exceeding 20% for a concentration of 2 mg/mL.The IC50 test values are provided in Table 4. Arborescin demonstrates modest antioxidant activity, evidenced by an IC50 value of 5.04 ± 0.12, significantly distant from that of ascorbic acid, which gives an IC50 value of 0.16 ± 0.003 mg/mL.Consistent with the pattern observed in most polyphenolic antioxidants, the configuration and total number of hydroxyl groups in the flavonoid structure significantly influence their antioxidant activity [56].This observation may justify the moderate antioxidant capacity found for arborescin in this study.

Bleaching Kinetics of the β-Carotene Assay
The results of arborescin's ability to inhibit or delay lipid peroxidation are presented in Figure 9.The results suggest that this particular compound lacks significant inhibitory effects on the oxidation of linoleic acid when compared to the negative control.It is noteworthy that the inhibition of oxidation is very low for arborescin, with a percentage of 3.64%.The observed value remains notably lower compared to that of the reference compound BHA (Table 4).It can be observed that the absorbance of arborescin and the negative control decrease linearly over time, unlike that of BHA.Even molecules with a weak or moderate antioxidant effect can be beneficial.Molecules with low antioxidant activity can indeed have a synergistic effect when combined with other antioxidants.If, for example, both molecules have moderate antioxidant properties, they can work together to regenerate each other and enhance overall antioxidant protection.Low antioxidant activity can also offer gradual and continuous protection against oxidative damage, which is especially beneficial for the long-term prevention of chronic diseases like cardiovascular diseases and certain cancers.
for example, both molecules have moderate antioxidant properties, they can work together to regenerate each other and enhance overall antioxidant protection.Low antioxidant activity can also offer gradual and continuous protection against oxidative damage, which is especially beneficial for the long-term prevention of chronic diseases like cardiovascular diseases and certain cancers.In the presence of the compound, the free radical of linoleic acid experiences an attack by β-carotene, resulting in rapid bleaching, which is attributed to the depletion of double bonds in linoleic acid [57].This is in contrast to BHA, where absorbance decreases gradually, indicating the inhibition of linoleic acid oxidation.

Antibacterial Activity
Evaluation of the antibacterial activity of the arborescin molecule revealed notable results (Table 5), highlighting its effectiveness against different bacterial strains.In the presence of the compound, the free radical of linoleic acid experiences an attack by β-carotene, resulting in rapid bleaching, which is attributed to the depletion of double bonds in linoleic acid [57].This is in contrast to BHA, where absorbance decreases gradually, indicating the inhibition of linoleic acid oxidation.

Antibacterial Activity
Evaluation of the antibacterial activity of the arborescin molecule revealed notable results (Table 5), highlighting its effectiveness against different bacterial strains.Minimum inhibitory concentrations (MICs) varied according to the bacteria tested, with values of 83 µg/mL for E. coli, 166 µg/mL for S. aureus and L. innocua, and again 83 µg/mL for P. aeruginosa.These data demonstrate the ability of arborescin to inhibit bacterial growth at relatively low concentrations.The minimum bactericidal concentration (MBC) reflects the lowest concentration at which the molecule completely eradicates bacteria.The MBC for E. coli is 333 µg/mL, while for S. aureus it is indicated as being greater than or equal to 666 µg/mL, demonstrating bactericidal activity at higher concentrations.For L. innocua, the MBC is 666 µg/mL, and for P. aeruginosa, it is 333 µg/mL.These results demonstrate the specific antibacterial activity of arborescin against different bacterial strains.It should be noted that this molecule is known for its diverse biological activities, such as its anticancer potential.Martins et al. [58] highlighted that arborescin, in particular, is the most promising compound, capable of reducing the viability of tumor cells.These results highlight the versatile potential of arborescin and encourage further investigation into its application, whether in a clinical or industrial context.

Antifungal Activity
Evaluation of the antifungal activity of the arborescin molecule produced notable results (Table 6), revealing its efficacy against various fungal strains.Minimum inhibitory concentrations (MICs) varied according to the strains tested, with values of 41 µg/mL for Aspergillus niger, 83 µg/mL for Candida glabrata, and again 41 µg/mL for Penicillium digitatum.These data indicate that arborescin has the capacity to inhibit fungal growth at relatively low concentrations.The minimum fungicidal concentration (MFC) represents the lowest concentration at which the molecule completely eradicates fungi.The MFC for Aspergillus niger is greater than or equal to 166 µg/mL, for Candida glabrata it is 41 µg/mL, and for Penicillium digitatum it is 166 µg/mL.
These results highlight the specific antifungal activity of arborescin against different fungal strains.They also indicate that the molecule's efficacy varies according to the type of fungus under consideration.This inherent specificity has significant implications for the future development of arborescin as an antifungal agent.The results serve as a catalyst for further research to elucidate the precise mechanism by which arborescin combats fungi.This investigation aims to discern its potential applications in clinical or industrial contexts, with a particular focus on the treatment of fungal infections.

Molecular Docking Predictions Revealing Potential Mechanisms of Action for Arborescin
To investigate the impact of arborescin, a sesquiterpene lactone, on pharmacological functions, we employed diverse computer-based techniques to conduct molecular docking with relevant molecular receptors.The binding strength correlates inversely with the numerical value of binding affinity, typically measured in kcal/mol.The initial docking prediction revealed an expected binding affinity, marked by a root mean square deviation of zero [38].
In this study, we utilized a method to evaluate the binding affinities of arborescin.The compound was tested against two important proteins associated with the tested biological functions: Xanthine oxidoreductase (PDB ID: 3AX7) was examined for antioxidant activity [43], and Dihydrofolate reductase (DHFR, PDB ID: 4M6J) was examined for its antibacterial activity, as reported by [59].The results of the molecular docking investigations are presented in Table 7.
Table 7.The computational simulations in CAEO yield the molecular free binding affinity of the identified compounds, measured in kcal/mol.Xanthine oxidoreductase (XOR) is an enzyme involved in purine metabolism, catalyzing the conversion of hypoxanthine to xanthine and subsequently to uric acid [60].While this enzyme plays a crucial role in normal physiological processes, such as the breakdown of nucleotides, it also generates reactive oxygen species (ROS) as byproducts, particularly during the conversion of hypoxanthine to xanthine and xanthine to uric acid [61].These ROS can lead to oxidative stress and contribute to various pathological conditions, including inflammation, cardiovascular diseases, and neurodegenerative disorders [62,63].
The significance of inhibiting XOR lies in its potential to reduce the production of ROS and subsequent oxidative damage [43].By inhibiting XOR activity, either through direct inhibition or modulation of its expression, it is possible to decrease ROS production, thereby mitigating oxidative stress and its associated detrimental effects [64].This inhibition can be particularly relevant in the context of antioxidant activity, as it helps to balance the oxidative state within cells and tissues.
Our investigation unveiled that arborescin exhibited a noteworthy binding affinity of −8.0 kcal/mol, akin to the native inhibitor allopurinol, which also displayed a binding affinity of −8.0 kcal/mol (refer to Table 7).Remarkably, arborescin engaged in four hydrogen bonds with amino acid residues situated within the binding site pocket, specifically Leu580, Glu757, Arg793, and Ile1063.In contrast, allopurinol did not form any hydrogen bonds with the amino acid residues within the binding pocket (Figure 10).3.10.2.Interactions with Dihydrofolate Reductase (PDB: 4M6J): Antibacterial Activity Dihydrofolate reductase (DHFR) serves a vital function in the synthesis of tetrahydrofolate, a crucial precursor required for the biosynthesis of purines, pyrimidines, and specific amino acids [65].Targeting DHFR inhibition has been a focal point in the development of antibacterial, antifungal, antituberculosis, and anticancer drugs due to its role in impeding cellular replication and growth [66,67].
The protein structure, identified by its PDB ID 4M6J, was retrieved from the RCSB Protein Data Bank and represents the crystallographic depiction of DHFR (Dihydrofolate Reductase) derived from Staphylococcus aureus [68].This structural revelation presents a compelling opportunity for the advancement of antibacterial treatments, particularly considering the virulent nature of S. aureus, a pathogenic bacterium known to cause a spectrum of infections including skin and soft tissue disorders, bone infections, and others [68].Focusing on DHFR emerges as a strategically pivotal pursuit as the inhibition of this enzyme interrupts the biosynthesis of tetrahydrofolate.This disruption ultimately leads to the depletion of crucial folate coenzymes necessary for the synthesis of nucleic acids, including DNA and RNA as well as certain amino acids [69].As a result, DHFR emerges as a captivating focal point for the progression of antibacterial medications, demonstrating the potential to effectively hinder bacterial growth and proliferation [70].
In this investigation, arborescin demonstrated a robust docking score of −8.1 kcal/mol, surpassing that of ciprofloxacin, a broad-spectrum antibiotic belonging to the fluoroquinolones group.This finding suggests the potential of arborescin as a potent antibacterial agent, as highlighted in Table 7.While ciprofloxacin formed three hydrogen bonds with amino acid residues within the protein's binding pocket-Ser3, Val109, and His130-arborescin only engaged in a single hydrogen bond with Arg137 at the active site, as depicted in Figure 11.3.10.2.Interactions with Dihydrofolate Reductase (PDB: 4M6J): Antibacterial Activity Dihydrofolate reductase (DHFR) serves a vital function in the synthesis of tetrahydrofolate, a crucial precursor required for the biosynthesis of purines, pyrimidines, and specific amino acids [65].Targeting DHFR inhibition has been a focal point in the development of antibacterial, antifungal, antituberculosis, and anticancer drugs due to its role in impeding cellular replication and growth [66,67].
The protein structure, identified by its PDB ID 4M6J, was retrieved from the RCSB Protein Data Bank and represents the crystallographic depiction of DHFR (Dihydrofolate Reductase) derived from Staphylococcus aureus [68].This structural revelation presents a compelling opportunity for the advancement of antibacterial treatments, particularly considering the virulent nature of S. aureus, a pathogenic bacterium known to cause a spectrum of infections including skin and soft tissue disorders, bone infections, and others [68].Focusing on DHFR emerges as a strategically pivotal pursuit as the inhibition of this enzyme interrupts the biosynthesis of tetrahydrofolate.This disruption ultimately leads to the depletion of crucial folate coenzymes necessary for the synthesis of nucleic acids, including DNA and RNA as well as certain amino acids [69].As a result, DHFR emerges as a captivating focal point for the progression of antibacterial medications, demonstrating the potential to effectively hinder bacterial growth and proliferation [70].
In this investigation, arborescin demonstrated a robust docking score of −8.1 kcal/mol, surpassing that of ciprofloxacin, a broad-spectrum antibiotic belonging to the fluoro-quinolones group.This finding suggests the potential of arborescin as a potent antibacterial agent, as highlighted in Table 7.While ciprofloxacin formed three hydrogen bonds with amino acid residues within the protein's binding pocket-Ser3, Val109, and His130-arborescin only engaged in a single hydrogen bond with Arg137 at the active site, as depicted in Figure 11.The potential integration of arborescin, a sesquiterpenic lactone that has shown significant antimicrobial potential, could play a crucial role in the treatment and prevention of various pathogenic infections.Indeed, the compounds isolated from species of Asteraceae, belonging to the sesquiterpenic lactones family, have a wide range of biological effects; some of these compounds are already available on the market, such as artemisinin [71], which has shown anticancer [72] and antimalarial effects [73].
In some cases, sesquiterpene lactones are regarded as the primary active components of these medicinal plants [74].Some of these compounds were found to have antitumoral [75], anti-inflammatory, antimicrobial, antimalarial [76], neuroprotective [77], immunostimulant, and hepatoprotective properties [78].Studies conducted with arglabine demonstrate its potential for the development of new anticancer and antiinflammatory drugs [79].Cynaropicrin, falling within the same category of compounds, has demonstrated antitumor, antitrypanosomal, and anti-inflammatory properties, alongside its antiviral efficacy against hepatitis C [80].Costunolide has been proven to be a potent anticancer agent and anti-inflammatory, as well as exhibiting antimicrobial and antidiabetic properties [81,82].
In terms of the relationship between the structure and activity of these compounds, it seems that the α-methylene-γ-lactone nucleus plays a vital role in most of their documented biological effects [16,83].
Due to its functional groups, Artemetin holds promise for evaluation as a therapeutic agent with potential anti-inflammatory, antidiabetic, and anticancer properties, offering a diverse range of pharmaceutical potentials.
The main limiting factor in this perspective is the relatively low yield generated by the isolation process.

Conclusions
Bioactive molecules serve as a central focus in the exploration of aromatic and medicinal plants, owing to their notable biological activities.A lot of compounds possess intricate structures that confer upon them elevated levels of both chemical and biological reactivity.Our research specifically delves into the properties of "Arborescin," a member of the sesquiterpenic lactone family extracted from A. absinthium L. The extraction process The potential integration of arborescin, a sesquiterpenic lactone that has shown significant antimicrobial potential, could play a crucial role in the treatment and prevention of various pathogenic infections.Indeed, the compounds isolated from species of Asteraceae, belonging to the sesquiterpenic lactones family, have a wide range of biological effects; some of these compounds are already available on the market, such as artemisinin [71], which has shown anticancer [72] and antimalarial effects [73].
In some cases, sesquiterpene lactones are regarded as the primary active components of these medicinal plants [74].Some of these compounds were found to have antitumoral [75], anti-inflammatory, antimicrobial, antimalarial [76], neuroprotective [77], immunostimulant, and hepatoprotective properties [78].Studies conducted with arglabine demonstrate its potential for the development of new anticancer and anti-inflammatory drugs [79].Cynaropicrin, falling within the same category of compounds, has demonstrated antitumor, antitrypanosomal, and anti-inflammatory properties, alongside its antiviral efficacy against hepatitis C [80].Costunolide has been proven to be a potent anticancer agent and antiinflammatory, as well as exhibiting antimicrobial and antidiabetic properties [81,82].
In terms of the relationship between the structure and activity of these compounds, it seems that the α-methylene-γ-lactone nucleus plays a vital role in most of their documented biological effects [16,83].
Due to its functional groups, Artemetin holds promise for evaluation as a therapeutic agent with potential anti-inflammatory, antidiabetic, and anticancer properties, offering a diverse range of pharmaceutical potentials.
The main limiting factor in this perspective is the relatively low yield generated by the isolation process.

Conclusions
Bioactive molecules serve as a central focus in the exploration of aromatic and medicinal plants, owing to their notable biological activities.A lot of compounds possess intricate structures that confer upon them elevated levels of both chemical and biological reactivity.Our research specifically delves into the properties of "Arborescin", a member of the sesquiterpenic lactone family extracted from A. absinthium L. The extraction process involved the use of hexane, after refluxing with a mixture of acetone and water.Mass spectroscopy, infrared spectroscopy, scanning electron microscope, X-ray energy dispersive spectroscopy, and CNHSO elemental analysis were conducted to elucidate the molecular structure; then, thermal analysis was performed for the arborescin molecule (C 2 OH 2 OO 8 ) and rigorously examined.Following structural identification and determination of the quantum parameters of the descriptors, the antifungal and antibacterial activity of arborescin was evaluated, and the results show that it possesses interesting antimicrobial activity against the strains used, which has been correlated to docking.

Separations 2024 ,
11,  x FOR PEER REVIEW 4 of 22 removed.They were then stored in a cool environment (4 °C) for later use.Just before use, the dried plant was reduced to a powder.

Figure 1 .
Figure 1. A. absinthium L. plant harvesting from the city of Oujda Angad in Morocco's southwest Oriental region.

Figure 1 .
Figure 1. A. absinthium L. plant harvesting from the city of Oujda Angad in Morocco's southwest Oriental region.
contains characteristic absorption bands in the range of 3058 and 2910 cm −1 , which are indicative of C-H bonds.There is a more intense and sharper band at 1770 cm −1 characteristic of the carbonyl in the γ-lactone, a band at 1430 cm −1 which corresponds to deformation vibrations in the plane of C-H bonds, and a band at 1128 cm −1 attributed to the epoxy group C-О-C.Separations 2024, 11, x FOR PEER REVIEW 9 of 22There is a more intense and sharper band at 1770 cm −1 characteristic of the carbonyl in the γ-lactone, a band at 1430 cm −1 which corresponds to deformation vibrations in the plane of C-H bonds, and a band at 1128 cm −1 attributed to the epoxy group С-О-С.
. The MS spectrum provides the following ions: An ion detected at m/z 233, representing a loss of 15 u from m/z 248, implies the potential loss of CH3• An ion observed at m/z 96, which corresponds to a loss of 152 u from m/z 248, indicates the potential loss of a fragment with the molecular formula of C9H12O2 2• An ion at m/z 69 (loss of 179.07 u from m/z 288) suggesting the loss of C10H11O3 3• An ion at m/z 190, indicating a loss of 44 u from m/z 233, suggests the potential loss of CHO2 3• An ion at m/z 147 (loss of 43 u from m/z 190) suggesting the loss of C2H3O 3• An ion at m/z 107, signifying a loss of 40 u from m/z 147, suggests the loss of C3H4 2• An ion at m/z 215, indicative of a loss of 44 u from m/z 233, suggests the loss of CHO2 3• These findings offer invaluable insights into the molecular structure and fragmentation patterns of the compound, enabling a comprehensive characterization of its molecular composition.

Figure 6 .
Figure 6.Various possible fragments of the MS spectrum.

Figure 6 . 2 • 3 • 3 •
Figure 6.Various possible fragments of the MS spectrum.An ion detected at m/z 233, representing a loss of 15 u from m/z 248, implies the potential loss of CH 3 • An ion observed at m/z 96, which corresponds to a loss of 152 u from m/z 248, indicates the potential loss of a fragment with the molecular formula of C 9 H 12 O 2 2• An ion at m/z 69 (loss of 179.07 u from m/z 288) suggesting the loss of C 10 H 11 O 3 3• An ion at m/z 190, indicating a loss of 44 u from m/z 233, suggests the potential loss of CHO 2 3• An ion at m/z 147 (loss of 43 u from m/z 190) suggesting the loss of C 2 H 3 O 3• An ion at m/z 107, signifying a loss of 40 u from m/z 147, suggests the loss of C 3 H 4 2•

Figure 7 .
Figure 7. (A) The optimized configuration of arborescin in its neutral state alongside (B) the Highest Occupied and the Lowest Unoccupied Molecular Orbitals determined by DFT.

Figure 7 .
Figure 7. (A) The optimized configuration of arborescin in its neutral state alongside (B) the Highest Occupied and the Lowest Unoccupied Molecular Orbitals determined by DFT.

Figure 8 .
Figure 8. Scavenging activity of the DPPH radical in the presence of arborescin.Figure 8. Scavenging activity of the DPPH radical in the presence of arborescin.

Figure 8 .
Figure 8. Scavenging activity of the DPPH radical in the presence of arborescin.Figure 8. Scavenging activity of the DPPH radical in the presence of arborescin.

Figure 9 .
Figure 9. Kinetics of β-carotene bleaching in the presence of arborescin and BHA.

Figure 9 .
Figure 9. Kinetics of β-carotene bleaching in the presence of arborescin and BHA.

Table 1 .
Atomic chemical composition of the isolated crystal.

Table 1 .
Atomic chemical composition of the isolated crystal.
Probable chemical structure of the isolated crystal.

Table 3 .
Quantum parameters of global reactivity descriptors calculated.

Table 4 .
IC50 values obtained in the presence of arborescin for the DPPH and FRAP assays, along with the percentage Relative Antioxidant Activity (% RAA) for the β-carotene bleaching assay.

Table 6 .
Assessment of Antifungal Activity.