Inﬂuence of Orchard Cultural Practices during the Productive Process of Cherries through Life Cycle Assessment

: This study describes the inﬂuence of orchard cultural practices during the productive process of cherries on the environmental impact in terms of energy, air, soil and water through a “farm to market” Life Cycle Assessment (LCA). The results were used to identify the orchard cultural practices that contribute signiﬁcantly to the environmental impact and to ﬁnd solutions to reduce those impacts, serving as best practices guide to improving the environmental performance and as benchmarks for other national and international cherry and fruit growers. Primary data for production, harvest and post-harvest periods were gathered experimentally. The openLCA 1.10.2 software and the ecoinvent 3.5 database were used for modelling. Test case scenarios are modelled to identify the inﬂuence of cultural practices in low and high cherry production campaigns depending on climatic conditions and consequently diseases and plagues. Moreover, results are compared with other studies, not only covering cherries but also other fruits. The energy consumption per hectare in the production phase is similar in test scenarios. The energy consumption of orchard cultural practices related to tractor use, fertilizers and fungicides application are the main hotspots in terms of global warming, freshwater ecotoxicity and eutrophication, and terrestrial acidiﬁcation. The use of electric vehicles, change the warehouse location or redeﬁne transportation routes can reduce this impact, along with the optimization of the cherry’s quantity transported in each trip. In addition, the use of plant protection products, fertilizers and herbicides with less environmental impact will contribute to this objective. For that, the use of agriculture and precision systems to predict the need for fertilizers (nutrients), herbicides and fungicides, the use of decision support systems to deﬁne the dates of cultural practices, as well as innovative and emerging food and by-products processing methods are suggested. Thus, this study identiﬁes and quantiﬁes the environmental impacts associated with the production system of cherries and their main hotspots. It provides a best-practices guide for sustainable solutions in orchard management that contributes to the competitiveness and sustainability of fruit companies. warehouse’s diesel fuel, herbicides, transportation from the warehouse to the retailer’s diesel fuel, fertilizers and tractor’s diesel fuel environmental impacts.


Introduction
One of the most discussed topics nowadays is the environment and the impact of agricultural activities. So, in recent years, efforts have been made in several areas to develop sustainable alternatives that can replace or improve the current ones [1].
In recent years, the evolution from traditional practices to intensive agriculture in order to increase plantation productivity has led to environmental impacts, such as resource depletion, soil erosion and global warming, among others [2]. The agri-food sector has frequently been considered one of the major sectors with the highest environmental impact [3]. Over the years, intensive agriculture has been adopted with increasing frequency towards

Goal and Scope
The primary goal of the study is to evaluate the energetic, air, soil and water impacts resulting from the productive system and post-harvest of cherry of Fundão "from farm to market." It aims to detect the main hotspots that can be changed or manipulated to reduce their respective environmental impacts. The results will be compared with other studies, not only about cherries but also about other fruits. The cherry with PGI represents almost half of the country's production in the center region of Portugal; thus, it is fundamental to analyze its environmental impact. In addition, life cycle assessment studies are increasingly being required to find more sustainable solutions for orchard management solutions; therefore, this study can bring a great contribution to the cherry sector.

Functional Unit
The functional unit (FU) is a measure of performance and has a reference function since it allows quantifying the results of orchard production so they can be comparable. In studies of fruit products, the definition of FU can be a complex process since this approach can be conducted in terms of quality or economy [22]. The FU for a product system, particularly in agriculture, can be mass-based or land-based, and they will provide different but interesting results. The comparison of the same production system across different FUs can lead to quite different scenarios. Fruit cultivars with a higher yield demonstrate a much better environmental performance using a mass-based FU, while fruit cultivars with lower productivity show better results with a land-based FU [22]. Some authors such as Tabatabaie and Murthy [23] and Sanderson et al. [24] opted for a massbased FU (1 kg), and other authors such as Tricase et al. [25] and Ingrao et al. [26] chose a land-based FU (1 ha).
The FU defined for the energy consumption of cherry production was 1 hectare of an orchard. The choice fell on a land-based FU since the operations in the orchard are not production-dependent. For the rest of the environmental impact indicators, it was used 1 kg of produced cherry as a mass-based FU. This choice is aligned with the option of Gaspar et al. [27].

System Boundaries
The system boundaries define which processes and inputs are considered in the LCA study [23]. The system boundaries are defined through an iterative process where the starting point is chosen, and then, the next selected processes are added to be part of the system according to their importance and relevance for the study. This delimitation is very complex, and different authors define different boundaries. This difference in the selection of the limits and boundaries of the system for the LCA results in some problems, such as the difficulty in comparing the results of several studies since they do not cover the same processes [28].
The cherry LCA boundaries are "from farm to market," and the limits are divided into two phases: cultivation/production period and harvest and post-harvest period. The cut-off criteria for these phases were to define and select the most relevant operations for the study taking into account environmental aspects in terms of energy, water, soil and air impact that can be simultaneously measured quantitatively and in a rigorous and objective way.

Inventory Analysis
This phase consists of collecting data and performing the necessary calculations to determine in quantitative terms all relevant inputs and outputs within the boundaries of the system [29]. It can be the most time-consuming phase due to the data collection since it is dependent on a good database and the availability of suppliers and customers to cooperate in the investigation [10].
This study requires a very strict and detailed data quality. That is why all the primary data for production, harvest and post-harvest periods were collected from a regional farm, which possesses 20 ha of cherry trees, but only 14 of those ha were considered, which were the ones in full production.
This type of localized cherry production, due to its characteristics, enjoys a strong reputation and is considered to have economic, agricultural, and gastronomic importance in the region. The production area of this cherry, in which the regional farm is inserted, provides very favorable edaphoclimatic conditions to the development. The existence of many cold hours during the winter, a mild spring, a hilly area that protects from the wind, granite soils and slope shale, coupled with the local producers' know-how, result in the attributes of this cherry [30].
The annual cherry production of the farm was 10 tonnes/ha for each of the 14 ha in full production. The study considers the plantation area (5 m × 3 m) per tree, the number of trees per row and the number of rows where operations of the productive system occurred. The study only covers the impacts associated with one cherry production year because a complete study would require data of approximately 20 years to monitor the cherry tree lifecycle, i.e., from the plantation of the cherry tree until the tree removal. As the company was founded 9 years ago, that data was not available. To do this study, the openLCA 1.10.2 software (GreenDelta GmbH, Berlin, Germany) and the ecoinvent 3.5 database and the Life Cycle Impact Assessment (LCIA) method were also used.
However, there are some limitations to the employed tools. OpenLCA is a tool that uses methods and databases downloaded separately from other sources, and the quality of the results strongly depends on which database is used and for what purpose. Another limitation is the necessity for reference or comparison data in the used databases, which often require to be added by the user. Finally, LCIA has also some limitations, such as not every environmental area is referred to by the default method, and only some elementary flow are characterized, thus being difficult to include all their potential impacts.
This LCA study considers operations during the production and post-harvest phases. In the production phase, the operations of pruning, soil maintenance, spraying of plant protection products, irrigation, herbicide and fertilizer applications were considered. The post-harvest phase covers the operations of refrigerated storing, processing, packaging and transportation of the cherry. These limits can be observed in Figure 1.
flow are characterized, thus being difficult to include all their potential impacts.
This LCA study considers operations during the production and post-harvest phases. In the production phase, the operations of pruning, soil maintenance, spraying of plant protection products, irrigation, herbicide and fertilizer applications were considered. The post-harvest phase covers the operations of refrigerated storing, processing, packaging and transportation of the cherry. These limits can be observed in Figure 1.

Production Phase
The pruning is performed by electric pruning shears, which are connected to lithium batteries that consume 0.144 kWh. On average, a cherry tree in full production takes 5 min to go through maintenance pruning. Therefore, if 1 ha has 666 plants and 1 plant in full production is pruned in 5 min, the pruning lasts 55.5 h/ha.
The soil maintenance is conducted by two techniques: the sown cover crop between the lines and no-till soil maintenance in the lines of the orchard. To do the maintenance by the sown cover crop, a shredder/weeding machine is used, which takes, on average, 4 h/ha, and this operation is conducted two times per year. The no-till soil technique consists of the application of herbicides, specifically glyphosate. The herbicides are applied two times per year by a pulverization spraying machine, and it takes 2 h/ha per application.
The spraying of plant protection products consists on the application of fungicides and insecticides to protect the orchard against plague attacks and diseases. There are 7 applications of these products during the year, where 6 of them are applications of fungicides that occur simultaneously with 3 applications of insecticides. In addition, an application of insecticides is performed separately. Each application of fungicides and insecticides takes 1 h/ha. The drip irrigation system is used in the analyzed farm because it is a localized system that always maintains the soil with the necessary humidity, generating good yields. The water is distributed in low intensity and high frequency directly in the root zone of the plants through the drippers. The water irrigation system consumes 2028 m 3 /ha during the year.
The fertilizers sprayed onto the soil are nitrogen, potassium, phosphorus, zinc, sulfur, magnesium, boron and calcium. The application lasts 1 h/ha, and it is made once a year.

Harvest and Post-Harvest Phase
The harvest is manually made by hand, and the harvested cherries are placed in boxes that are transported by 2 or 3 diesel fuel vans to the warehouse, where they are stored, processed and packaged. The warehouse is located between the parcels, which

Production Phase
The pruning is performed by electric pruning shears, which are connected to lithium batteries that consume 0.144 kWh. On average, a cherry tree in full production takes 5 min to go through maintenance pruning. Therefore, if 1 ha has 666 plants and 1 plant in full production is pruned in 5 min, the pruning lasts 55.5 h/ha.
The soil maintenance is conducted by two techniques: the sown cover crop between the lines and no-till soil maintenance in the lines of the orchard. To do the maintenance by the sown cover crop, a shredder/weeding machine is used, which takes, on average, 4 h/ha, and this operation is conducted two times per year. The no-till soil technique consists of the application of herbicides, specifically glyphosate. The herbicides are applied two times per year by a pulverization spraying machine, and it takes 2 h/ha per application.
The spraying of plant protection products consists on the application of fungicides and insecticides to protect the orchard against plague attacks and diseases. There are 7 applications of these products during the year, where 6 of them are applications of fungicides that occur simultaneously with 3 applications of insecticides. In addition, an application of insecticides is performed separately. Each application of fungicides and insecticides takes 1 h/ha. The drip irrigation system is used in the analyzed farm because it is a localized system that always maintains the soil with the necessary humidity, generating good yields. The water is distributed in low intensity and high frequency directly in the root zone of the plants through the drippers. The water irrigation system consumes 2028 m 3 /ha during the year.
The fertilizers sprayed onto the soil are nitrogen, potassium, phosphorus, zinc, sulfur, magnesium, boron and calcium. The application lasts 1 h/ha, and it is made once a year.

Harvest and Post-Harvest Phase
The harvest is manually made by hand, and the harvested cherries are placed in boxes that are transported by 2 or 3 diesel fuel vans to the warehouse, where they are stored, processed and packaged. The warehouse is located between the parcels, which means that the transportation is variable. If the orchard is close to the warehouse, the trip is only a few meters away, and it is conducted by foot. On the other hand, the trip can take 5-10 min by van if the orchard is further away. These trips from the orchard to the warehouse are frequent, making it impossible to concentrate a large production in the field to avoid sun exposure and high temperatures. During the harvesting, the tractor is not used.
The cherries are stored in refrigeration chambers until they are transported to the retailer. Refrigeration plays an important role in preserving the properties of the cherry in the post-harvest phase and maintaining an adequate temperature and relative humidity. The cherry should be stored at temperatures between 0 • C and 4 • C in an atmosphere with relative humidity between 90% and 95% [31]. The company stores the cherry at temperatures between 2 • C and 3 • C, and the relative humidity is 90%. Therefore, it is possible to verify that the cooling conditions in the company are within the recommended parameters. This phase is very important to preserve the quality and the properties of the cherry.
InovEnergy (2012) includes the energy power consumptions for the cold storage of horticulture products of several companies in the Center region of Portugal. A linear relationship between the energy power consumption and the number of workers and tons of produced products was determined. Considering the company's data close to very similar to those of specific farm that made part of this study (3 refrigeration chambers, annual production of 435 tons and 4 permanent workers), an energy power consumption of 25 521 kWh was considered for fruit storage at the company, resulting in energy consumption of 58.67 kWh/ton.
Finally, the transportation of the cherries from the warehouse to the retailer is subcontracted and is carried out by a truck that takes 2-3 h to complete the trip of 200-250 km. During the trip, the cherries are transported at temperatures between 2 • C and 5 • C, and the relative humidity of the air is between 80% and 90%, which are conditions very close to the ideal in order to preserve the quality of the product.

Input Flow
An in-depth investigation of the orchard system was performed to collect all the necessary data and identify the most relevant inputs inventories for cherries LCA. Therefore, energy inputs and emissions considered were from the fuel consumed in transport and in the orchard's machinery, storage in the warehouse due to the refrigeration system, irrigation water and all plant protection products (fungicides and insecticides), herbicides and fertilizers applied in the soil.
To convert all the inputs considered into their respective energy equivalents, expressed in MJ/ha, the coefficients in Table 1 were used.  [25] All the inputs considered in this cherries LCA are exposed in Table 2.  The fuel consumption for the production phase is difficult to calculate since the power of agricultural machines is very variable, as its consumption. According to IEA [34], the density of diesel fuel in Portugal is 0.837 kg/L. Therefore, the diesel fuel consumption of agricultural machinery was calculated using this value for a 60 horsepower (hp) tractor. According to Grisso et al. [35], it is possible to obtain the fuel consumption of the tractor as shown by Equation (1). where: C ijk = Fuel consumption j during operation i execution using machine k (L/h); P ik = Power of the machine k used in operation i (hp).
Therefore, the fuel consumption of agricultural machinery during the production phase was 199.8697 L/ha. According to Pereira et al. [36] and using values of the "EMEP/EEA Air Pollutant Emission Inventory Guidebook 2019" report, the emissions resulting from fuel burning can be calculated using three methods: Tier 1, Tier 2 and Tier 3. Tier 1 is the most basic method because it requires the least amount of information, while Tier 2 is suitable for more complex situations and in countries where specific emission factors are available. Tier 3 is the most complex method and requires access to a much larger amount of information and data. The emissions from fuel burning of agricultural machinery used during the production phase were calculated by Tier 2, as shown in Equation (2) [37]. where: E pollutant = Specific emissions for each pollutant (g); FC fuel category = Fuel consumption for each fuel category (kg); EF pollutant = Emission factor for each fuel category (g/kg).
The emissions from the transportation of the cherry can be divided into two different parts, one directed for the Light Commercial Vehicles, LCV < 3.5 tons and the other to the Heavy-Duty Vehicles, HDV > 3.5 tons. Emission factors were taken from the report "EMEP/EEA Air Pollutant Emission Inventory Guidebook 2019" considering the category Euro 3 for the LCV (registration of the vehicle between 2000 and 2004) and Euro 6 to HDV (registration of the vehicle after 2014) [38]. The remaining data for the transportation in the company is shown in Table 3. The calculation of the emissions from the transportation of the cherry was made using the Tier 2 of the "EMEP/EEA Air Pollutant Emission Inventory Guidebook 2019" report, as shown by Equation (3) [38].
where: The energy power consumed in the storage of the cherry is also a source of emissions for the air. According to IEA [34], for the gross production of electricity and heat for the years 2012-2014, an average emission factor of 0.322 kg CO 2 /kWh of energy power consumed considering its respective transmission and distribution was defined. Therefore, these emissions resulting from the storage of the cherry are calculated by Equation (4) [39]. where: E energy power = CO 2 emissions to air from the energy power consumed for the storage of the cherry (kg CO 2 /ton of produced cherry); C energy power = Energy power consumed for the storage of the cherry (kWh/ton of produced cherry).
In addition to soil emissions, fertilizers are also a source of CO 2 eq into the air. According to Hughes et al. [40], the emission factor for nitrogen fertilizers is 6.163 kg CO 2 eq /kg of nitrogen fertilizer. Therefore, CO 2 emissions can be calculated by Equation (5). where: Processes 2021, 9, 1065 9 of 24 E nitrogen fertilizer = CO 2 emissions to the air from nitrogen fertilizer applied into the soil (kg CO 2 eq /ha); Q nitrogen fertilizer = Quantity of nitrogen fertilizer applied into the soil (kg of nitrogen fertilizer/ha).
The phosphorus fertilizer also emits CO 2 into the air. According to Hughes et al. [40], the emission factor for phosphorus fertilizer is 1.859 kg CO 2 eq /kg of phosphorus fertilizer. Therefore, CO 2 emissions can be calculated by Equation (6).
where: E phosphorus fertilizer = CO 2 emissions to the air from phosphorus fertilizer applied into the soil (kg CO 2 eq /ha); Q phosphorus fertilizer = Quantity of phosphorus fertilizer applied into the soil (kg of phosphorus fertilizer/ha).
The potassium fertilizer is also a source of CO 2 emissions to the air. According to Hughes et al. [40], the emission factor is 1.770 kg CO 2 eq /kg of potassium fertilizer. Therefore, CO 2 emissions can be calculated by Equation (7). where: E potassium fertilizer = CO 2 emissions to the air from potassium fertilizer applied into the soil (kg CO 2 eq /ha); Q potassium fertilizer = Quantity of potassium fertilizer applied into the soil (kg of potassium fertilizer/ha).
In addition to the emissions mentioned before from nitrogen fertilizers, there still are other direct and indirect emissions to the air resulting from that fertilizer. The direct emissions are due to the degradation of organic matter, releasing nitrogen fixed in the soil. These direct impacts, according to the "Portuguese National Inventory Report on Greenhouse Gases, 1990-2017", can be calculated by Equation (8) [41]. The nitrogen fertilizer also has indirect N 2 O emissions to the air due to the volatilization and atmospheric deposition of the nitrogen applied into the soil. The nitrogen is volatilized in the form of NH 3 and NO x , and, sometimes, a fraction of that volatilized nitrogen returns to the soil, and it is reemitted as N 2 O. Therefore, according to the "Portuguese National Inventory Report on Greenhouse Gases, 1990-2017", the indirect N 2 O emissions resulting from the application of nitrogen fertilizers into the soil can be calculated by Equation (8) [41].
where: The indirect emissions from nitrogen fertilizer applied into the soil was calculated using Equation (9).
where: Fungicides applied into the soil are also a source of GHG emissions to the air. According to [40], the fungicides emission factor is 3.303 kg CO 2 eq /kg of fungicides. Therefore, CO 2 emissions can be calculated by Equation (10). where: E fungicides = CO 2 emissions to the air from fungicides applied into the soil (kg CO 2 eq /ha); Q fungicides = Quantity of fungicides applied into the soil (kg of fungicides/ha).
Insecticides applied into the soil also emit CO 2 to the air. According to [40], the insecticides emission factor is 4.744 kg CO 2 eq /kg of insecticides. Therefore, CO 2 emissions can be calculated by Equation (11). where: E insecticides = CO 2 emissions to the air from insecticides applied into the soil (kg CO 2 eq /ha); Q insecticides = Quantity of insecticides applied into the soil (kg of insecticides/ha).
Herbicides applied into the soil are also a source of CO 2 emissions to the air. According to [40], the herbicides emission factor is 5.076 kg CO 2 /kg of herbicides. Therefore, CO 2 emissions can be calculated by Equation (12). where: E herbicides = CO 2 emissions to the air from herbicides applied into the soil (kg CO 2 /ha); Q herbicides = Quantity of herbicides applied into the soil (kg of herbicides/ha).

Test Case Scenarios
The annual cherry production is very variable because it depends on the growing season and the climatic conditions, as they can be favorable or not to the development of diseases and plagues. In the agricultural region of Beira Interior (Portugal), the cherry registered substantial falls in production in some growing seasons that varied between approximately 50% (fall registered in 2007) and 60% (fall registered in 2016). These production falls were mainly due to adverse climatic conditions and plague attacks. On the other hand, there are also years, such as in 2011 and 2015, when conditions were favorable, resulting in a total production increase between 25% and 50% [42].
Therefore, two alternative scenarios were defined with the goal of making an analysis for the low and high cherry production scenarios. In scenario 1, the low production scenario, a decrease of 50% of production, from 10 tons/ha to 5 tons/ha, is considered due to adverse climatic conditions, the attack of plagues and the development of diseases in the orchard. This scenario will necessarily lead to an increase in the number of sprayings of plant protection products. In scenario 2, the high production scenario, an increase of 50% of production (from 10 tons/ha to 15 tons/ha) is considered due to favorable climatic conditions for the growth of the cherry, leading to a decrease in the number of sprayings of plant protection products.
The consumption of agricultural operations during the production phase remains the same in both scenarios because they do not depend on the produced quantity, except for the number of plant protection products sprayings. In the post-harvest phase, the same operations as in the real scenario are considered.

Results of Life Cycle Assessment
A few environmental impact indicators were analyzed, such as global warming, freshwater ecotoxicity, freshwater eutrophication, terrestrial acidification and terrestrial ecotoxicity for the Fundão's cherry LCA. The global warming indicator was calculated using the CML 2001 method, while the remaining indicators were calculated using the ReCiPe 2008 Midpoint (E) method. The option for using two impact assessment methods fell on the fact that the CML 2011 method is more adequate to evaluate the global warming indicator while the ReCiPe 2008 Midpoint (E) method is more complete and suitable for the other indicators. Sanderson et al. [24] also conducted their study according to this division of methods. The results are shown in Table 4.

Energy Consumption
All the results regarding the energy consumption for the real and test scenarios are shown in Figure 2. The energy consumption for the real scenario is 29,956 MJ/ha. The spraying of plant protection products operation (including the diesel fuel consumed in this operation) is the most energy-consuming operation, being responsible for 8,241 MJ/ha (27.5%) of the total energy consumption. This component is followed by the application of herbicide (including the diesel fuel consumed in this operation) with 5,488 MJ/ha (18.3%), the application of fertilizers (including the diesel fuel consumed in this operation) with 5,292 MJ/ha (17.7%), the soil maintenance by sown cover crop with 4,502 MJ/ha (15.0%), the transportation with 3,015 MJ/ha (10.1%), the warehouse's energy power with 2,112 MJ/ha (7.1%), the irrigation water with 1,278 MJ/ha (4.3%) and the pruning with 29 MJ/ha (0.1%). Diesel fuel accounts for 47.6% of the global energy consumption (14,270 MJ/ha). Comparing these results with other fruits, it appears that the proportion of 10.1% of transport in global energy consumption is quite close to the result obtained of 12.3% by Gaspar et al. [27] for Beira Interior's peach. In that same study, diesel fuel also has a very significant proportion of 28.9% in total energy consumption, although it is lower than the present study of Fundão's cherry. Tricase et al. [25] developed a study for the cherry in the Apulia region, Italy, where diesel fuel represents a considerable proportion of 59% of the total energy consumption, being in accordance with the results obtained in the present study. The value of 15.8% of the energy consumption of fertilizers is also in line with the value obtained of 12% in the study by [25]. Processes 2021, 9, x FOR PEER REVIEW 12 of 25 Comparing the results of the real scenario with the test scenarios, scenarios 1 (low production scenario) and 2 (high production scenario) do not differ much in terms of the proportions for each operation. Thus, the environmental impact during the production period can be considered the base line for the environmental impact during the post-harvest period. A slight decrease of 390 MJ/ha is predicted in scenario 1 to an energy consumption of 29,566 MJ/ha. It is also predicted a decrease of 982 MJ/ha in scenario 2, thus to an energy consumption of 28,974 MJ/ha. Scenario 1 shows a slight increase in the energy consumption of the plant protection products spraying operation with a proportion of 34.5% of the global energy consumption, followed by the application of herbicides (18.6%) and the application of fertilizers (17.9%). The remaining energy consumption is distributed by the soil maintenance by sown cover crop (15.2%), transportation (5.1%), warehouse's energy power (4.3%), irrigation water (4.3%) and pruning (0.1%). The proportion of diesel fuel consumption remains practically unchanged, standing at 47% of global energy consumption. Scenario 2 shows a slight decrease in the energy consumption of the plant protection products spraying operation to 17.7% of the global energy consumption. Therefore, the application of herbicides becomes the operation with the highest energy consumption with 18.9% of the global energy consumption, followed by the application of fertilizers (18.3%), transportation (15.6%), soil maintenance by sown cover crop (15.5%), warehouse's energy power (9.4%), irrigation water (4.4%) and pruning (0.1%). The proportion of diesel fuel consumption remains practically unchanged, increasing only 1% to 48.6% of global energy consumption.
The main reason for the variations of the results for the test scenarios is the spraying of plant protection products, the operation with the larger energy consumption for the real scenario and scenario 1, due to the amounts of fungicides and insecticides applied into the soil and the diesel fuel consumed by the tractor during this operation. The difference in energy consumption related to the spraying of plant protection products is explained by the variation in the quantity of these products applied and the respective diesel fuel consumption of the tractor. In the real scenario, seven applications of these products were considered (six fungicides applications with three insecticides applications simultaneously and one more insecticide application separately). In scenario 1, the low production scenario requires nine applications of these products (six applications of fungicides with three applications of insecticides simultaneously, one application of fungicides and Comparing the results of the real scenario with the test scenarios, scenarios 1 (low production scenario) and 2 (high production scenario) do not differ much in terms of the proportions for each operation. Thus, the environmental impact during the production period can be considered the base line for the environmental impact during the post-harvest period. A slight decrease of 390 MJ/ha is predicted in scenario 1 to an energy consumption of 29,566 MJ/ha. It is also predicted a decrease of 982 MJ/ha in scenario 2, thus to an energy consumption of 28,974 MJ/ha. Scenario 1 shows a slight increase in the energy consumption of the plant protection products spraying operation with a proportion of 34.5% of the global energy consumption, followed by the application of herbicides (18.6%) and the application of fertilizers (17.9%). The remaining energy consumption is distributed by the soil maintenance by sown cover crop (15.2%), transportation (5.1%), warehouse's energy power (4.3%), irrigation water (4.3%) and pruning (0.1%). The proportion of diesel fuel consumption remains practically unchanged, standing at 47% of global energy consumption. Scenario 2 shows a slight decrease in the energy consumption of the plant protection products spraying operation to 17.7% of the global energy consumption. Therefore, the application of herbicides becomes the operation with the highest energy consumption with 18.9% of the global energy consumption, followed by the application of fertilizers (18.3%), transportation (15.6%), soil maintenance by sown cover crop (15.5%), warehouse's energy power (9.4%), irrigation water (4.4%) and pruning (0.1%). The proportion of diesel fuel consumption remains practically unchanged, increasing only 1% to 48.6% of global energy consumption.
The main reason for the variations of the results for the test scenarios is the spraying of plant protection products, the operation with the larger energy consumption for the real scenario and scenario 1, due to the amounts of fungicides and insecticides applied into the soil and the diesel fuel consumed by the tractor during this operation. The difference in energy consumption related to the spraying of plant protection products is explained by the variation in the quantity of these products applied and the respective diesel fuel consumption of the tractor. In the real scenario, seven applications of these products were considered (six fungicides applications with three insecticides applications simultaneously and one more insecticide application separately). In scenario 1, the low production scenario requires nine applications of these products (six applications of fungicides with three applications of insecticides simultaneously, one application of fungicides and two applications of insecticides separately) because of the adverse weather conditions, the attack of plagues and the development of diseases. Scenario 1 conditions necessarily cause an increase in the spraying of plant protection products and leads to an increase in diesel fuel of the tractor as a result of the increase in tractor's utilization by 2 h. In scenario 2, the high production scenario, four applications of these products were considered (four applications of fungicides with two applications of insecticides simultaneously) because of the favorable climatic conditions and the adverse conditions for the development of diseases and plague attacks. This condition leads to a decrease in the number of plant protection products spraying and the reduction of diesel fuel of the tractor as a result of the decrease in tractor's utilization by 3 h.

Global Warming
According to Smith et al. [43], the main greenhouse gas (GHG) emissions resulting from agriculture are carbon dioxide (CO 2 ), methane (CH 4 ) and nitrous oxide (N 2 O). These gases are considered to be the main contributors to global warming. In this study, in addition to these gases, nitrogen oxides (NO x ), sulfur dioxide (SO 2 ), Non-methane volatile organic compounds (NMVOCs), carbon monoxide (CO) and ammonia (NH 3 ) were also considered. Therefore, the global warming "from farm to market" impact of Fundão's cherry is 0.1536 kg CO 2 eq /kg of produced cherry. The main contributors to global warming are the tractor's diesel fuel, responsible for 0.0529 kg CO 2 eq /kg of produced cherry (34.4%), and the fertilizers with 0.0509 kg CO 2 eq /kg of produced cherry (33.1%). With less impact, the energy power for the warehouse contributes with 0.0189 kg CO 2 eq /kg of produced cherry (12.3%), and the diesel fuel from transportation from the warehouse to the retailer is responsible for 0.0148 kg CO 2 eq /kg of produced cherry (9.6%), as shown in Figure 3.

Global Warming
According to Smith et al. [43], the main greenhouse gas (GHG) emissions resultin from agriculture are carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O). Thes gases are considered to be the main contributors to global warming. In this study, in ad dition to these gases, nitrogen oxides (NOx), sulfur dioxide (SO2), Non-methane volati organic compounds (NMVOCs), carbon monoxide (CO) and ammonia (NH3) were als considered.
Therefore, the global warming "from farm to market" impact of Fundão's cherry 0.1536 kg CO2 eq/kg of produced cherry. The main contributors to global warming are th tractor's diesel fuel, responsible for 0.0529 kg CO2 eq/kg of produced cherry (34.4%), an the fertilizers with 0.0509 kg CO2 eq/kg of produced cherry (33.1%). With less impact, th energy power for the warehouse contributes with 0.0189 kg CO2 eq/kg of produced cherr (12.3%), and the diesel fuel from transportation from the warehouse to the retailer is r sponsible for 0.0148 kg CO2 eq/kg of produced cherry (9.6%), as shown in Figure 3. These results expose a considerable difference between exhaust gases system of ag ricultural machinery that works outside paved roads and the vehicles, such as a van or truck, that operate on paved roads, resulting in a discrepancy between the two emission of diesel fuel consumed by the tractor (34.4%) and emissions from the LCV (1.18%) an the HDV (9.62%). Therefore, considering the transportation from the warehouse to th retailer represents 9.62% of the total impact, it could be recommended to change the war house location closer to the retailer or to search for alternatives to do this transportation  These results expose a considerable difference between exhaust gases system of agricultural machinery that works outside paved roads and the vehicles, such as a van or a truck, that operate on paved roads, resulting in a discrepancy between the two emissions of diesel fuel consumed by the tractor (34.4%) and emissions from the LCV (1.18%) and the HDV (9.62%). Therefore, considering the transportation from the warehouse to the retailer represents 9.62% of the total impact, it could be recommended to change the warehouse location closer to the retailer or to search for alternatives to do this transportation. The proportion of 33.1% of the total impact due to the use of fertilizers is also quite appreciable when compared to the impact resulting from the use of herbicides (4.5%), fungicides (4.0%) and insecticides (0.8%). This difference can be explained by the high use of nitrogen as a fertilizer because it is applied in larger quantities, and it has an emission factor (6.163 kg CO 2 eq /kg of nitrogen fertilizer applied into the soil) that is much higher compared to the other chemical compounds applied into the soil.
Analyzing the comparative results for the different operations for scenarios 1 and 2 shown in Figure 3, it is possible to verify that there is a drastic increase (almost double) of the environmental impact of global warming comparing the real scenario (0.1536 kg CO 2 eq /kg of produced cherry) with scenario 1, which shows an emission of 0.2901 kg CO 2 eq /kg of produced cherry. On the other hand, there is a slight decrease comparing the real scenario (0.1536 kg CO 2 eq /kg of produced cherry) with scenario 2 (0.1046 kg CO 2 eq /kg of produced cherry).
The impact of the diesel fuel consumption of the tractor increases its proportion to 40.1% of the total impact in scenario 1, and it decreases its influence by 28.7% in scenario 2. These variations are normal, considering that the tractor's use increases in scenario 1 due to the increase in the number of plant protection products sprayings (fungicides and insecticides), and these number of these sprayings decrease in scenario 2, reducing the use of the tractor. For the same reason, the impact of fungicides and insecticides increases in scenario 1 and decreases in scenario 2.

Freshwater Ecotoxicity
All the chemical compounds applied in the orchard, such as herbicides, fungicides, insecticides or fertilizers, can have negative consequences for the environment or human health. The chemical elements that compose these products can be toxic and volatilize to the air, be drained into the surface water and infiltrate into groundwater [44]. These compounds may suffer chemical/physical modifications and circulate between different ecosystems, having the ability to be retained in the soil, water and atmosphere but also in animal and human food, threatening not only human health but also all living organisms [45].
Therefore, the freshwater ecotoxicity impact of Fundão's cherry is 755.41 × 10 −5 kg 1.4-DCB eq /kg of produced cherry. The main contributors to freshwater ecotoxicity are the fungicides with 458.53 × 10 −5 kg 1.4-DCB eq /kg of produced cherry (60.7%) and the fertilizers with 281.09 × 10 −5 kg 1.4-DCB eq /kg of produced cherry (37.2%). Then, in an almost residual impact, insecticides contribute with 14.59 × 10 −5 kg 1.4-DCB eq /kg of produced cherry (1.9%), and the herbicides are responsible for 1.2 × 10 −5 kg 1.4-DCB eq /kg of produced cherry (0.2%), as shown in Figure 4 and Table 5. The proportion of 33.1% of the total impact due to the use of fertilizers is also quite appreciable when compared to the impact resulting from the use of herbicides (4.5%), fungicides (4.0%) and insecticides (0.8%). This difference can be explained by the high use of nitrogen as a fertilizer because it is applied in larger quantities, and it has an emission factor (6.163 kg CO2 eq/kg of nitrogen fertilizer applied into the soil) that is much higher compared to the other chemical compounds applied into the soil.
Analyzing the comparative results for the different operations for scenarios 1 and 2 shown in Figure 3, it is possible to verify that there is a drastic increase (almost double) of the environmental impact of global warming comparing the real scenario (0.1536 kg CO2 eq/kg of produced cherry) with scenario 1, which shows an emission of 0.2901 kg CO2 eq/kg of produced cherry. On the other hand, there is a slight decrease comparing the real scenario (0.1536 kg CO2 eq/kg of produced cherry) with scenario 2 (0.1046 kg CO2 eq/kg of produced cherry).
The impact of the diesel fuel consumption of the tractor increases its proportion to 40.1% of the total impact in scenario 1, and it decreases its influence by 28.7% in scenario 2. These variations are normal, considering that the tractor's use increases in scenario 1 due to the increase in the number of plant protection products sprayings (fungicides and insecticides), and these number of these sprayings decrease in scenario 2, reducing the use of the tractor. For the same reason, the impact of fungicides and insecticides increases in scenario 1 and decreases in scenario 2.

Freshwater Ecotoxicity
All the chemical compounds applied in the orchard, such as herbicides, fungicides, insecticides or fertilizers, can have negative consequences for the environment or human health. The chemical elements that compose these products can be toxic and volatilize to the air, be drained into the surface water and infiltrate into groundwater [44]. These compounds may suffer chemical/physical modifications and circulate between different ecosystems, having the ability to be retained in the soil, water and atmosphere but also in animal and human food, threatening not only human health but also all living organisms [45].
Therefore, the freshwater ecotoxicity impact of Fundão's cherry is 755.41 × 10 −5 kg 1.4-DCB eq/kg of produced cherry. The main contributors to freshwater ecotoxicity are the fungicides with 458.53 × 10 −5 kg 1.4-DCB eq/kg of produced cherry (60.7%) and the fertilizers with 281.09 × 10 −5 kg 1.4-DCB eq/kg of produced cherry (37.2%). Then, in an almost residual impact, insecticides contribute with 14.59 × 10 −5 kg 1.4-DCB eq/kg of produced cherry (1.9%), and the herbicides are responsible for 1.2 × 10 −5 kg 1.4-DCB eq/kg of produced cherry (0.2%), as shown in Figure 4 and Table 5.  It can be verified that the fungicides and fertilizers together account for 97.9% of the total freshwater ecotoxicity impact. Fungicides represent a large proportion (60.7%) of the total impact due to the higher quantities applied into the soil compared with the insecticides and herbicides. Although fertilizers were applied in larger quantities than fungicides, they have a minor impact because the copper oxychloride, one of the fungicides applied, has a very high emission factor (3.4846 kg 1.4-DCB eq /kg of copper oxychloride applied into the soil) compared to the others.
Analyzing the comparative results for the different operations for scenarios 1 and 2 shown in Figure 4, there is a substantial increase (more than double) of the freshwater ecotoxicity impact comparing the real scenario (755.41 × 10 −5 kg 1.4-DCB eq /kg of produced cherry) with scenario 1, which presents an emission of 1620.73 × 10 −5 kg 1.4-DCB eq /kg of produced cherry. On the other hand, there is a considerable decrease (almost half) comparing the real scenario with scenario 2 (405.82 × 10 −5 1.4-DCB eq /kg of produced cherry).
In a more detailed analysis, the impact of the fungicides slightly increases its expression to 61.6% of the total impact in scenario 1 and decreases its influence to 52.4% in scenario 2. These variations are normal, considering that in scenario 1, there is an increase in the number of sprayings of plant protection products (fungicides and insecticides). In scenario 2, there is a decrease in those sprayings, reducing their impacts. For the same reason, the impact of insecticides increases in scenario 1 and decreases in scenario 2.

Freshwater Eutrophication
Freshwater eutrophication consists of the overgrowth of aquatic plants or the uncontrolled multiplication of algae as a result of high levels of nutrients in freshwater ecosystems. The main cause for the development of this phenomenon is the contamination by nutrients in the form of phosphorus from agricultural fertilizers, sewage effluents and leakage of urban rainwater. When the accumulation of phosphorus exceeds a certain limit, a recycling mechanism is activated, and the system remains closed in a eutrophic state even when nutrient inputs are considerably reduced [46]. Therefore, the freshwater eutrophication impact of Fundão's cherry is 0.0025 kg P eq /kg of produced cherry. The total impact of freshwater eutrophication is due to the use of fertilizers, more specifically phosphorus. The nutrient phosphorus is responsible for the total impact on this indicator, as shown in Figure 5. The impact increases to 0.0050 kg P eq /kg of produced cherry in scenario 1 due to the increase in the amount of phosphorus applied into the soil. On the other hand, the impact decreases in scenario 2 to 0.0017 kg P eq /kg of the produced cherry because the quantity of phosphorus applied into the soil also decreases.
Peq/kg of produced cherry. The total impact of freshwater eutrophication is due to the of fertilizers, more specifically phosphorus. The nutrient phosphorus is responsible the total impact on this indicator, as shown in Figure 5. The impact increases to 0.0050 Peq/kg of produced cherry in scenario 1 due to the increase in the amount of phospho applied into the soil. On the other hand, the impact decreases in scenario 2 to 0.0017 Peq/kg of the produced cherry because the quantity of phosphorus applied into the s also decreases.

Terrestrial Acidification
Terrestrial acidification consists of the modification of the chemical properties of soil as a result of the deposition of nutrients such as nitrogen and sulfur in acidify forms. This deposition can result from the emissions of NOx, NH3 and SO2 into the which, in addition to acidifying the pH of the soil, causes a decline in the soil fertility t can lead to the discoloration of plant tissues, germination of new seeds and decrease root production. This phenomenon culminates in the decrease in photosynthetic rates a in extreme cases, in the decrease in plant biodiversity [47].
Therefore, the terrestrial acidification impact of Fundão's cherry is 942.27 × 10 −7 SO2 eq/kg of produced cherry. The main contributors to terrestrial acidification are the tilizers with 664.56 × 10 −7 kg SO2 eq/kg of produced cherry (70.5%) and the tractor's die fuel with 266.51 × 10 −7 kg SO2 eq/kg of produced cherry (28.3%). Then, in an almost resid impact, the diesel fuel consumed in the transportation from the warehouse to the reta is responsible for 7.64 × 10 −7 kg SO2 eq/kg of produced cherry (0.8%), and the diesel f consumed in the transportation from the orchard to warehouse accounts for 3.55 × 10 − SO2 eq/kg of produced cherry (0.4%), as shown in Figure 6.

Real Scenario
Scenario 1 Scenario 2 kg P eq/kg of cherry produced

Terrestrial Acidification
Terrestrial acidification consists of the modification of the chemical properties of the soil as a result of the deposition of nutrients such as nitrogen and sulfur in acidifying forms. This deposition can result from the emissions of NO x , NH 3 and SO 2 into the air, which, in addition to acidifying the pH of the soil, causes a decline in the soil fertility that can lead to the discoloration of plant tissues, germination of new seeds and decrease in root production. This phenomenon culminates in the decrease in photosynthetic rates and, in extreme cases, in the decrease in plant biodiversity [47].
Therefore, the terrestrial acidification impact of Fundão's cherry is 942.27 × 10 −7 kg SO 2 eq /kg of produced cherry. The main contributors to terrestrial acidification are the fertilizers with 664.56 × 10 −7 kg SO 2 eq /kg of produced cherry (70.5%) and the tractor's diesel fuel with 266.51 × 10 −7 kg SO 2 eq /kg of produced cherry (28.3%). Then, in an almost residual impact, the diesel fuel consumed in the transportation from the warehouse to the retailer is responsible for 7.64 × 10 −7 kg SO 2 eq /kg of produced cherry (0.8%), and the diesel fuel consumed in the transportation from the orchard to warehouse accounts for 3.55 × 10 −7 kg SO 2 eq /kg of produced cherry (0.4%), as shown in Figure 6. It can be verified that the fertilizers and tractor's diesel fuel together account for 98.8% of the total terrestrial acidification impact. Fertilizers represent a large proportion (70.5%) due to the amounts of SO2 applied into the soil. The tractor's diesel fuel also emits large amounts of SO2 due to its combustion, justifying its proportion in the total impact (28.3%). As stated before, the vehicles operating on paved roads have better exhaust gas It can be verified that the fertilizers and tractor's diesel fuel together account for 98.8% of the total terrestrial acidification impact. Fertilizers represent a large proportion (70.5%) due to the amounts of SO 2 applied into the soil. The tractor's diesel fuel also emits large amounts of SO 2 due to its combustion, justifying its proportion in the total impact (28.3%). As stated before, the vehicles operating on paved roads have better exhaust gas systems, which is the reason why the diesel fuel of transport from the warehouse to the retailer and from the orchard to the warehouse causes an almost residual impact (0.8% and 0.4%, respectively).
Analyzing the comparative results for the different operations for scenarios 1 and 2 shown in Figure 6, there is a large increase (more than double) of the terrestrial acidification impact comparing the real scenario (942.27 × 10 −7 kg SO 2 eq /kg of produced cherry) with scenario 1, which presents an emission of 1,926.64 × 10 −7 kg SO 2 eq /kg of produced cherry. On the other hand, there is also a considerable decrease (almost half) comparing the real scenario with scenario 2 (605.26 × 10 −7 kg SO 2 eq /kg of produced cherry).
In a more detailed analysis, the impact of the tractor's diesel fuel increases its proportion to 30.4% in scenario 1 and decreases to 25.0% in scenario 2. These variations are due to the increase in the number of sprayings of plant protection products (fungicides and insecticides) in scenario 1 that causes an increase in tractor's use, while that impact decreases in scenario 2 because the number of those same sprayings decrease, reducing the tractor's utilization too.

Terrestrial Ecotoxicity
Similar to freshwater ecotoxicity, terrestrial ecotoxicity consists of the impact of toxic substances on human health and biodiversity, in this case, on terrestrial ecosystems. Products applied into the soil, such as plant protection products or fertilizers, emit toxic substances that affect soil-dependent organisms and their ecosystems [48].
Therefore, the terrestrial ecotoxicity impact of Fundão's cherry is 3894.26 × 10 −5 kg 1.4-DCB eq /kg of produced cherry. The main contributors to terrestrial ecotoxicity are the fungicides with 3724.98 × 10 −5 kg 1.4-DCB eq /kg of produced cherry (95.7%). The remaining impact is distributed by the fertilizers with 113.80×10 −5 kg 1.4-DCB eq /kg of produced cherry (2.9%), insecticides with 54.03 × 10 −5 kg 1.4-DCB eq /kg of produced cherry (1.4%) and the herbicides with 1.45 × 10 −5 kg 1.4-DCB eq /kg of produced cherry (0.04%), as shown in Figure 7 and Table 6.    The fungicides are responsible for 95.7% of the total impact. This substantial proportion is due to the copper oxychloride applied onto the soil because it has a considerable emission factor (30.45 kg 1.4-DCB eq /kg of produced cherry) compared with the other chemical compounds.
Analyzing the comparative results for the different operations for scenarios 1 and 2 shown in Figure 7, there is a large increase (more than double) of the terrestrial ecotoxicity impact comparing the real scenario (3894.26 × 10 −5 kg 1.4-DCB eq /kg of produced cherry) with scenario 1, which presents an emission of 8037.72 × 10 −5 kg 1.4-DCB eq /kg of produced cherry. There is also a considerable decrease (more than half) comparing the real scenario with scenario 2 (1766.08 × 10 −5 kg 1.4-DCB eq /kg of produced cherry). These variations are normal, considering that in scenario 1, there is an increase in the number of sprayings of plant protection products (fungicides and insecticides), causing an increase in the impact of these products. That same impact decreases in scenario 2 because of the number of those sprayings decreases.

Comparison with Results in Literature
The review of Clune et al. [49] compiles a considerable number of LCA studies of cherry and other products and defined its boundary from cradle to the regional distribution center, that is, from planting the cherry tree to the transportation of the cherry to the regional distribution center. The FU used was 1 kg of produced cherry. The transportation of the cherry to the regional distribution center in a recent study developed by Clune et al. [49] is considered equivalent to the transportation from the warehouse to retailer in the present study. These authors reached three values for the global warming indicator: 0.26, 0.29 and 0.88 kg CO 2 eq /kg of minimum, average and maximum values of produced cherry, respectively. It is necessary to highlight that this review considers both cherry and sour cherry. The present study has a substantially lower comparative value (0.1536 kg CO 2 eq /kg of produced cherry), even below the minimum value of that review. Some factors contribute to these results, such as the transportation distance between the warehouse and the retailer being shorter than in that study, the implementation of good environmental practices such as the use of electric pruning shears instead of pneumatic pruning shears, the use of a fully electric irrigation system and the fact that the harvesting process is conducted manually and without the use of the tractor.
The study of Sanderson et al. [24] "from cradle to market" defined the FU of 1 kg of marketable cherry; that is, only cherries deemed for sale were considered, excluding the ones that were disposed of due to low quality. This study presents the value for the global warming indicator of 0.30096 kg CO 2 eq /kg of marketable cherry and did not account for 16.4% of the cherries disposed of in the packaging operation because it did not meet the requirements to be put up for sale. The study of Svanes and Johnsen [17] "from cradle to market" limit and the FU of 1 kg of cherry consumed shows a value of 0.53 kg CO 2 eq /kg of cherry consumed. The present study of Fundão's cherry is quite below the values of these two studies.
Comparing the results with other fruits, the value obtained in the present study is lower than the result of 0.23 kg CO 2 eq /kg of produced apple and 0.29 kg CO 2 eq /kg of produced peach in the study of Vinyes et al. [50] for apple and peach and "from cradle to retail" limit. Another study "from cradle to retail" for the apple of Keyes et al. [51] shows a value of 0.28 kg CO 2 eq /kg of produced apple for the global warming indicator.
Comparing the study of Svanes and Johnsen [17], which determined the value of 0.74 kg CO 2 eq for the plum in a "from cradle to market" limit for the FU of 1 kg of consumed plum but did not consider the 12.7% of the low-quality disposed plum, it is also possible to verify that the present study for the cherry shows a substantially lower value. In addition to the good practices at the company previously mentioned, other possible explanations for these results are the non-inclusion of the "cradle" phase and the electricity consumed in the irrigation system in this Fundão's cherry LCA. Figure 8 shows the comparative values of the global warming indicator for the studies mentioned above.
market" limit and the FU of 1 kg of cherry consumed shows a value of 0.53 kg CO2 of cherry consumed. The present study of Fundão's cherry is quite below the valu these two studies.
Comparing the results with other fruits, the value obtained in the present stu lower than the result of 0.23 kg CO2 eq/kg of produced apple and 0.29 kg CO2 eq/kg o duced peach in the study of Vinyes et al. [50] for apple and peach and "from crad retail" limit. Another study "from cradle to retail" for the apple of Keyes et al. [51] s a value of 0.28 kg CO2 eq/kg of produced apple for the global warming indicator. Com ing the study of Svanes and Johnsen [17], which determined the value of 0.74 kg CO2 the plum in a "from cradle to market" limit for the FU of 1 kg of consumed plum bu not consider the 12.7% of the low-quality disposed plum, it is also possible to verify the present study for the cherry shows a substantially lower value. In addition to the practices at the company previously mentioned, other possible explanations for the sults are the non-inclusion of the "cradle" phase and the electricity consumed in th gation system in this Fundão's cherry LCA. Figure 8 shows the comparative values of the global warming indicator for the ies mentioned above.  Therefore, it can be concluded that, although more LCA studies are needed for the cherry in Portugal in order to solidify these results, the global warming indicator "from farm to market" of Fundão's cherry is lower comparing with studies from other countries.
The freshwater ecotoxicity indicator of Fundão's cherry is 755.41 × 10 −5 kg 1.4-DCB eq /kg of produced cherry. This value is very similar to the result obtained in the study of [24] "from cradle to market" that shows the value for the freshwater ecotoxicity indicator of 763 × 10 −5 kg 1.4-DCB eq /kg of marketable cherry.
The freshwater eutrophication indicator of Fundão's cherry is 0.0025 kg P eq /kg of produced cherry. This value is slightly higher than the result of 0.00050 kg P eq /kg of marketable cherry obtained in the study of Sanderson et al. [24] "from cradle to market" that did not consider 16.4% of the produced cherry because it was disposed of due to its low quality. Comparing with the study of Svanes and Johnsen [17] that presents a value of 0.0045 kg PO 4 eq /kg of cherry consumed for limit "from cradle to market" and an FU of 1 kg of cherry consumed, the present study shows a slightly lower value. Comparing the results of the freshwater eutrophication indicator with other fruits, it is possible to verify that the result obtained in the present study is slightly higher than the result of 0.0018 kg PO 4 eq /kg of apple consumed and lower than the result of 0.0054 kg PO 4 eq /kg of plum consumed in the study of Svanes and Johnsen [17] with a "from cradle to market" limit. This study did not consider 12.7% of plums disposed of and 15.3% of apples disposed of that did not meet selling requirements.
The terrestrial acidification indicator of Fundão's cherry is 9.42 × 10 −5 kg SO 2 eq /kg of produced cherry. This value is substantially lower than the result of 167 × 10 −5 kg SO 2 eq /kg of marketable cherry obtained in the study of Sanderson et al. [24] "from cradle to market" that did not consider 16.4% of the produced cherry because of its disposal due to low quality. The Fundão's cherry LCA also shows a much lower value than the result of 690 × 10 −5 kg SO 2 eq /kg of cherry consumed in the study of Svanes and Johnsen [17] with a "from cradle to market" limit. Comparing the results of the terrestrial acidification indicator with other fruits, the results obtained in the present study is also inferior to the result of 250 × 10 −5 kg SO 2 eq /kg of apple consumed and the result of 610 × 10 −5 kg SO 2 eq /kg of plum consumed in the study of Svanes and Johnsen [17] "from cradle to market" limit. As previously mentioned, this study did not consider 12.7% of plums disposed of and 15.3% of apples disposed of because of theirs low quality.
The terrestrial ecotoxicity indicator of Fundão's cherry is 3894.26 × 10 −5 kg 1.4-DCB eq /kg of produced cherry. This value is substantially higher than the result of 58 × 10 −5 kg 1.4-DCB eq /kg of marketable cherry obtained in the study of Sanderson et al. [24] "from cradle to market" that did not consider 16.4% of the produced cherry because it did not meet the selling requirements.
Therefore, the majority of the indicators are lower than the results of the studies analyzed here. These results can be explained by several factors, such as the national environmental policies related to the agriculture and agribusiness sector. The legislation and financial support granted by the government, combined with new innovative techniques, allow achieving a very positive environmental performance. One of the examples of this effort in terms of environmental performance is the "Roadmap for Carbon Neutrality 2050 (RNC 2050)" whose main objective is to make Portugal a carbon-neutral country, that is, the balance between GHG emissions and carbon sequestration is neutral [7]. Portugal is even below the European average in terms of GHG emissions from agriculture, among other indicators [52]. In addition, the small size of the country and, consequently, the travelled distances also being shorter, contribute to a more adequate environmental performance.

Analysis and Discussion of Results
This Fundão's cherry LCA study "from farm to market" analyses the impacts in four parameters: energetic, water, air and soil impact. Therefore, the relevant environmental aspects of the production, harvest and post-harvest phases that could have an impact on these parameters within the defined limits were considered in order to promote the improvement of the environmental performance for the entire production system of Fundão's cherry.
The energy consumption led to the conclusion that, for the production phase, the energy consumption per hectare is the same regardless of the production, except for the spraying of plant protection products operation. The global energy consumption is 29,956 MJ/ha for the real scenario. Comparing these results, there are no significant variations in the global energy consumption between the real scenario, scenario 1 (29,566 MJ/ha) and scenario 2 (28,974 MJ/ha).
The global warming indicator is 0.1536 kg CO 2 eq /kg of produced cherry, showing the tractor's diesel fuel (34.4%) and the fertilizers (33.1%) as the main hotspots. Comparing these results, scenario 1 reveals a considerable increase (0.2901 kg CO 2 eq /kg of produced cherry), and scenario 2 shows exactly the opposite, that is, a considerable decrease (0.1046 kg of CO 2 eq /kg of produced cherry). In addition, the use of other plant protection products, fertilizers and herbicides with less environmental impact or reducing the number of the spraying of these compounds could also be a solution to reduce the environmental impact.
The water impact assessment was evaluated by the indicators of freshwater ecotoxicity and freshwater eutrophication.
The freshwater ecotoxicity indicator is 755.41 × 10 −5 kg 1.4-DCB eq /kg of produced cherry. The main contributors to freshwater ecotoxicity are the fungicides with 60.7% of the total impact and the fertilizers with 37.2%. For this indicator, scenario 1 shows a substantial increase compared to the real scenario (1620.73 × 10 −5 kg 1.4-DCB eq /kg of produced cherry), and scenario 2 shows a considerable decrease (405.82 × 10 −5 kg 1.4-DCB eq /kg of produced cherry). The freshwater eutrophication indicator is 0.0025 kg P eq /kg of produced cherry. The freshwater eutrophication impact is caused by the use of phosphorus fertilizers. Comparing with the real scenario, the impact increases to 0.0050 kg P eq /kg of produced cherry in scenario 1 due to the increase in the amount of phosphorus applied into the soil and, in the opposite direction, it decreases in scenario 2 to 0.0017 kg P eq /kg of produced cherry because that same amount of phosphorus applied in the soil also decreases. The replacement of phosphorus-based fertilizers with another chemical element cannot be a solution to reduce this environmental impact since phosphorus is one of the macronutrients that has a very important role in plant growth and development; therefore, it is not possible to make the replacement by another chemical element. However, phosphorus release could be controlled in a more efficient manner in order to limit phosphorus losses, and, in turn, use less fertilizer [53,54].
The soil impact assessment was evaluated by the indicators of terrestrial acidification and terrestrial ecotoxicity. The terrestrial acidification indicator is 942.27 × 10 −7 kg SO 2 eq /kg of produced cherry. The main contributors to terrestrial acidification are the fertilizers (70.5%) and the tractor's diesel fuel (28.3%). For this indicator, scenario 1 shows a considerable increase compared to the real scenario (1926.64 × 10 −7 kg SO 2 eq /kg of produced cherry) and scenario 2 also shows a substantial decrease to 605.26 × 10 −7 kg SO 2 eq /kg of produced cherry). The terrestrial ecotoxicity is 3894.26 × 10 −5 kg 1.4-DCB eq /kg of produced cherry. The main contributors to this impact are fungicides (95.7%). Comparing with the real scenario, the impact increases to 8037.72 × 10 −5 kg 1.4-DCB eq /kg of produced cherry in scenario 1, and it decreases to 1766.08 × 10 −5 kg 1.4-DCB eq /kg of produced cherry in scenario 2. The use of electric vehicles could mitigate this impact, along with the optimization of the cherry's quantity transported in each trip. In addition, the use of other plant protection products, fertilizers and herbicides with less environmental impact or reducing the number of sprayings of these compounds could also be a solution to reduce the environmental impact.
Therefore, in general, there is an increase in the environmental impact indicators in scenario 1 and a decrease in scenario 2. These variations are mainly explained by the same reason, that is, the increase of the number of sprayings of plant protection products and tractor's operating time in scenario 1 and the reduction of these two factors in scenario 2. So, although there is a higher consumption of transportation and storage in scenario 2 due to the higher production, the environmental impacts of this scenario are minor due to the fewer sprayings of plant protection products (reduction of the impact of fungicides, insecticides and tractor's diesel fuel).
Additionally, the company analyzed in this study shows a very positive environmental performance for Fundão's cherry, even greater than other cherry's LCA studies. The company has already implemented a few positive practices to reduce energy consumption and environmental impacts, such as the use of electric pruning shears instead of pneumatic pruning shears and the use of a fully electric irrigation system instead of using a diesel fuel pump. A possible suggestion for an even better environmental performance is to change the warehouse location to one closer to the retailer or to define alternatives for the transportation from the warehouse to the retailer, such as the use of electric vehicles. In addition, it is possible to choose other hypotheses to improve environmental performance, such as the use of agriculture and precision systems to identify the effective need for fertilizers (nutrients), herbicides and fungicides, the use of decision support systems to define the dates of agricultural operations to reduce their environmental impact and the optimization of the performance of the refrigeration systems of the chambers, which can be the simple explanation of good practices to the employees.

Conclusions
A cherries LCA study "from farm to market" is conducted to analyse the impacts in four parameters: energetic, water, air and soil impact. Results allow identifying the orchard cultural practices that contribute significantly to the environmental impact. Low and high cherry production scenarios that depend on climatic conditions and that are influenced by diseases and plagues are modelled. Best practices are described to improving the environmental performance the energy consumption per hectare in the production phase is similar in test scenarios. The energy consumption of orchard cultural practices related to tractor use, fertilizers and fungicides application are the main hotspots in terms of global warming, freshwater ecotoxicity and eutrophication, and terrestrial acidification.
Results show that transportation is an essential factor for reducing the global warming potential effects of agriculture. The expected accelerated increase regarding agricultural land occupation gravely highlights the importance of performing LCA studies. Therefore, farming practices such as fertilizer management, fuel use, and cultivation are quite significant. Likewise, options among transport methods and supply chain strategies carry important consequences for the global warming potential. This reality highlights the importance for policy makers to promote the best farming practices and transportation in a carbon-constrained world. Policies shaped towards climate change mitigation are gradually being informed by the results of greenhouse gas LCA from different areas of economic activity.
In order to stimulate the scientific knowledge and to strengthen the results obtained in this LCA study, there are some suggestions for future work: conducting LCA studies of other cherry farms in the region of Beira Interior; conducting studies with more in-depth limits and boundaries, preferably "from cradle to grave" studies, that is, an analysis of all stages from the plantation of the cherry tree until the moment that the cherry reaches the consumer; further analysis of the cooling system and the irrigation system; inclusion of disposal waste from the production system.
LCA studies should be carried out on biological or intensive fruit productive systems, such as vineyards or small fruits (red fruits), among others, to promote the competitiveness and sustainability of agricultural companies.