Research Trends on the Application of Electrolyzed Water in Food Preservation and Sanitation

: Electrolyzed water (EW) has been proposed as a novel promising sanitizer and cleaner in recent years. It is an effective antimicrobial and antibioﬁlm agent that has several advantages of being on the spot, environmentally friendly, cheap, and safe for human beings. Therefore, EW has been applied widely in various ﬁelds, including agriculture, food sanitation, livestock management, medical disinfection, clinical, and other ﬁelds using antibacterial technology. Currently, EW has potential signiﬁcance for high-risk settings in hospitals and other clinical facilities. The research focus has been shifted toward the application of slightly acidic EW as more effective with some supplemental chemical and physical treatment methods such as ultraviolet radiations and ultrasound. This review article summarizes the possible mechanism of action and highlights the latest research studies in antimicrobial applications.


Introduction
Foodborne disease refers to any illness caused by spoilage of contaminated food with pathogenic bacteria, parasites, or viruses [1,2]. Foodborne diseases take a significant toll on health [3] and are still not under control, and the epidemic may cause health concerns and economic losses [4]. The most common causes of foodborne illness are diarrheal disease agents, particularly Campylobacter spp. and norovirus [5]. However, there is no single clinical syndrome for all foodborne diseases [6]. The World Health Organization (WHO) estimates that there are nearly 1.7 billion cases of diarrheal disease in children each year, mainly caused by contaminated water and food. In addition, diarrheal disease caused 230,000 deaths cases and is mainly caused by non-typhoidal Salmonella enterica in 2010 [7]. Therefore, developing effective sanitizers for killing pathogens is one of the most critical food safety steps in agricultural and food products for the hazard analysis and critical control point (HACCP) principle.
Currently, various approaches have been used to eradicate foodborne pathogens while maximizing maintenance quality. Numerous commercial sanitizers, such as physical technology (ozone), chemical technology (chlorine compounds, peroxide mixtures, quaternary ammonium compounds), and biological technology (essential oil), have been used as disinfection techniques throughout the food chain [8][9][10][11][12]. However, some of these technologies in the food field are not entirely acceptable due to disadvantages such as chemical residues, limited inactivation, adverse effects on food quality, or potential toxicity to human beings or the environment. Thus, safe and effective disinfectants in the food industry are urgently needed.
Electrolyzed water (EW) containing hypochlorous acid (HOCl) has been proposed as a novel promising sanitizer and cleaner in recent years, as shown in the Figure 1. Sodium hypochlorite and EW are advanced chlorine-based sanitizers. The active compound of sodium hypochlorite solution is a chemical compound with the formula NaOCl that acted hypochlorite and EW are advanced chlorine-based sanitizers. The active compound of sodium hypochlorite solution is a chemical compound with the formula NaOCl that acted with carbon dioxide to produce hypochlorous acid in order to assure the safety of the products. However, EW is a solution produced by electrolyzing diluted electrolytes usually containing NaCl in an electrolysis cell, and the main microbial inactivation component is free chlorines species [13,14]. The electrolysis cell is the location where positively charged (anode) and negatively charged (cathode) electrodes were separated by a nonselective membrane [15]. EW comprises five types according to the final solution's pH value and has pH ranges including strongly acidic EW (StAEW) (pH 2.2-2.7), weakly acidic EW (WAEW) (pH 2.7-5.0), slightly acidic EW (SAEW) (pH 5.0-6.5), neutral EW (NEW) (pH 6.5-7.5), and alkaline EW(AkEW) (pH 11.0-13.8). EW showed antimicrobial activity against a wide range of microorganisms and eliminated the most common forms of bacteria, fungi, viruses, and spores in food, food processing surface, and non-food-contact surfaces in a relatively short time (usually within 10-20 s) [16][17][18]. Various studies have reported EW's antimicrobial effect in vegetables, fruits, poultry, and seafood [19][20][21]. Additionally, EW with available chlorine concentration was used instead of tap water during seed soaking and germination of seeds (mung bean, pea, brown rice, alfalfa, and buckwheat) [22][23][24][25]. This process resulted in inhibition of microbial growth, effects on morphological characteristics of sprouts, and even accumulation of functional components [26]. The antimicrobial activity of EW is currently evaluated in the medical area, such as venous ulcers in the legs, diabetic foot ulcers, or feminine hygiene [14]. Scientists also pay more attention to the EW applied in the clinical field (medical sterilization) due to antiinfection and cell proliferative effect.
Hurdle technology was applied as an intelligent combination of two or more preservatives techniques at less severe levels [27]. However, some studies have demonstrated that EW's application is limited by factors such as organics materials (lipids, proteins, etc.) and corrosion of equipment in contact with acidic or alkaline EW [28]. Therefore, combining EW with other disinfection methods (physical and chemical technology) could effectively achieve desirable results [29]. This paper aims to review recent developments on EW application including agriculture, food sanitation, and livestock management, especially for germination and the clinical field. In addition, the potential germicidal mechanisms and hurdle technology were discussed.

The Germicidal Mechanisms
Underlying microbial inactivation by EW have not been completely elucidated. However, a model roughly explaining germicidal mechanisms of EW has been developed, as shown in Figure 2. Chlorine species (HOCl, Cl2, and OCl − ) play an essential role in the The antimicrobial activity of EW is currently evaluated in the medical area, such as venous ulcers in the legs, diabetic foot ulcers, or feminine hygiene [14]. Scientists also pay more attention to the EW applied in the clinical field (medical sterilization) due to anti-infection and cell proliferative effect.
Hurdle technology was applied as an intelligent combination of two or more preservatives techniques at less severe levels [27]. However, some studies have demonstrated that EW's application is limited by factors such as organics materials (lipids, proteins, etc.) and corrosion of equipment in contact with acidic or alkaline EW [28]. Therefore, combining EW with other disinfection methods (physical and chemical technology) could effectively achieve desirable results [29]. This paper aims to review recent developments on EW application including agriculture, food sanitation, and livestock management, especially for germination and the clinical field. In addition, the potential germicidal mechanisms and hurdle technology were discussed.
antimicrobial efficacy of EW [30]. Briefly, EW showed its antimicrobial activity by attacking various cellular targets (cytoderm, intracellular components, and outer membrane). Firstly, HOCl can penetrate the cell membrane's lipid bilayer by passive diffusion due to its electrical neutrality and molecular size [31]. Cheng et al. reported that the bacterial surface was changed from smooth, consecutive, and bright into rough, shrunken, and even lysed after EW treatment [32]. In addition, Ding et al. reported that SAEW disrupted cell membrane permeability by damaging membrane proteins, entering the cells, and causing the agglutination of cellular inclusions [33]. Meanwhile, EW intensified membrane permeability and decreased the activity of 2, 3, 5-triphenyl tetrazolium chloride (TTC)-dehydrogenase, which resulted in the leakage of K + , DNA, and proteins [34]. EW attacking various cellular targets including cytoderm, intracellular components, and outer membrane. HOCl disordered intracellular components, normal cellular functions, and bacterial ultrastructures in various degrees including complex changes of intracellular metabolites, metabolic pathway (enzymatic level), and intracellular microenvironment (membrane integrity).
In addition to the chlorine family, other compounds such as reactive oxygen species (ROS) can also be produced in electrolysis, contributing to antimicrobial efficiency [35]. After EW penetrating the microbial cell, HOCl produced ROS (such as O − and OH − ) and Cl − , causing substantially disordered intracellular components, normal cellular functions, and bacterial ultrastructures in various degrees. (1) Including complex changes of intracellular metabolites, EW causes significant biochemical processes related to amino acid metabolism, peptidoglycan synthesis, nucleotides synthesis, and lipid biosynthesis [36,37]. Moreover, (2) in metabolic pathways (enzymatic level), EW shows negative effects on intracellular esterase activity, γ-aminobutyric acid (GABA) shunt, and enhanced glutamate decarboxylase (GAD) system for preventing oxidative stress and inactivating intracellular TTC-dehydrogenase [33,37,38]. (3) The intracellular microenvironment (membrane integrity) is also affected by breaking cell membrane integrity and decreasing membrane potentials to induce cell necrosis and apoptosis [38]. Cell membranes are the critical target for EW treatment of microorganisms [13].
In addition to lethal effects, EW could induce microorganisms to transfer into other physiological states, such as sublethal injury and viable but non-culturable (VBNC) state, In addition to the chlorine family, other compounds such as reactive oxygen species (ROS) can also be produced in electrolysis, contributing to antimicrobial efficiency [35]. After EW penetrating the microbial cell, HOCl produced ROS (such as O − and OH − ) and Cl − , causing substantially disordered intracellular components, normal cellular functions, and bacterial ultrastructures in various degrees. (1) Including complex changes of intracellular metabolites, EW causes significant biochemical processes related to amino acid metabolism, peptidoglycan synthesis, nucleotides synthesis, and lipid biosynthesis [36,37]. Moreover, (2) in metabolic pathways (enzymatic level), EW shows negative effects on intracellular esterase activity, γ-aminobutyric acid (GABA) shunt, and enhanced glutamate decarboxylase (GAD) system for preventing oxidative stress and inactivating intracellular TTC-dehydrogenase [33,37,38]. (3) The intracellular microenvironment (membrane integrity) is also affected by breaking cell membrane integrity and decreasing membrane potentials to induce cell necrosis and apoptosis [38]. Cell membranes are the critical target for EW treatment of microorganisms [13].
In addition to lethal effects, EW could induce microorganisms to transfer into other physiological states, such as sublethal injury and viable but non-culturable (VBNC) state, in which microorganisms could wait for resuscitation when the conditions become suitable for their growth. Liu et al. reported that the sublethal injured percentage of Listeria monocytogenes and Listeria innocua was 70% and 65%, respectively, after treatment of EW (8 ppm) [37].
Furthermore, EW can inhibit the activities of polyphenol oxidase activity (PPO) of fresh-cut vegetables such as sweet potato slices, mushrooms, and lotus root [38][39][40]. PPO, Processes 2021, 9,2240 4 of 20 which contributed to the brown reaction, catalyzes the oxidation of phenolic compounds into reactive quinones, and brown pigments were generated to quinones polymerization. The active compound (chlorine species: HOCl, Cl 2 , and OCl − ) of EW was considered to be the reason for the inhibition effect of PPO activity. EW can effectively inhibit microorganism growth and slow down the increase in TVB-N production of marine fish [41]. However, the exact pattern of EW on microbial cells is still unclear, and it requires more investigations to clarify its nature in the future.

Application of EW in Fruits and Vegetables
Pathogens can contaminate or colonize fruits and vegetables from preharvest production, distribution, processing, and packaging to food preparation. Microbial contamination of fruits and vegetables can cause spoilage of food and even human infection and poisoning. In order to ensure the safety of fruits and vegetables for human consumption, EW has been widely proved to be one of the most advanced treatments in vegetables. The sanitizing effect of various types of EW apply for vegetables and fruits is depicted in Table 1. Shen et al. reported the effectiveness of neutral EW treatment on apples (available chlorine concentration (ACC): 100 ppm, pH 6.9, and exposure time 2 min) against L. monocytogenes, Lactobacillus plantarum, Pediococcus acidilactici, and L innocua, Enterococcus faecium. A total reduction of 1.1 to 2.1 log CFU/g was achieved for all types of bacteria in apple. Puligundla et al. also reported reductions of 1.3 log for yeasts and molds and 1.4 log and 1.0 log CFU/g for total aerobic bacteria when broccoli sprout was dipped in AEW for 20 s [42].  Notes: ORP: Oxidation-reduction potential; EOW: electrolyzed oxidizing water. SAEW: slightly acidic electrolyzed water; AEW: acidic electrolyzed water; NEW: neutralized electrolyzed water; EW: electrolyzed water. ACC: available chlorine concentration.
Furthermore, Tang et al. reported that AEW treatments (ACC: 80 mg/L, pH 2.5, exposure time: 10 min) reduced disease occurrence in harvested longans. AEW treatment maintained high levels of non-enzymatic antioxidant systems and increased the activities of antioxidant enzymes to maintain longer shelf life [54]. The disinfection efficacy of SAEW treatment on wheat grains and pea sprout was also studied, and the total number of bacteria was reduced by approximately 0.65 to 1.58 log reductions, respectively [23,49]. Ogunniyi et al. reported that NEW was applied as a viable alternative to chemically based sanitizers for post-harvest treatment of spinach leaves [48].
The key factors are exposure time and temperature to reduce microbial count in fresh vegetables and fruits. Cap et al. evaluated the effectiveness of EW against Salmonella spp. in lettuce after short exposure times of 15 s, 30 s, and 45 s. The results revealed a Salmonella log reduction of 2.90 log CFU/g after 15 s and 30 s and 4.06 log CFU/g for treatment for 45 s [45]. However, Stopforth et al. studied AEW (ACC 50 ppm) in leafy greens and revealed no significant difference in the antibacterial effect above 1 min. Some other authors studied the effectiveness of EW for longer exposure times and reported similar or even lower bacterial reductions [55,56]. The reason may be that the dissociation of free chlorine or interference such as organic matter over a relatively short time [57]. Some other scientists reported that the storage temperature of fresh-cut vegetables should be below 5 • C in order to reduce the proliferation of spoilage and pathogenic microorganisms [58].

Application of EW in Poultry and Meat
Microbial contamination of poultry and meat products is a vital factor contributing to quality losses and foodborne illnesses. Many novel and effective chemical technologies, including EW treatment, have been used to reduce microbial contamination in meat and poultry. Around the world, 36% of all the meat consumed is pork, followed by poultry meat and beef at 35% and 22%, respectively [59].
EW's effectiveness has been reported against populations of S. enterica, E. coli, Yersinia inoculated, and Staphylococcus aureus, which are often associated with pork, beef, chicken, and other meat surfaces [60]. The decontamination efficiency of EW on poultry and meat products is depicted in Table 2. Recently, most studies have used soaking, spray, or immersion techniques with varying degrees of success [60][61][62]. Murad et al. achieved 1.5-1.6 logs CFU/g reduction in E. coli O157:H7 and Salmonella typhymurium on inoculated chickens and beef after 10 min of soaking in AEW treatment [63]. Wang et al. discovered that almost 1.0 log CFU/cm 2 microbial reduction was achieved by using novel spraying technology [62]. Han et al. studied the effectiveness of NEW on pork skin inoculated with E. coli O157:H7, Yersinia enterocolitica, and Salmonella. After 2 min of immersion treatment with NEW containing 74 ppm, total reductions of 2.12, 2.22, and 1.74 log CFU/g were recorded for E. coli O157: H7, S. enteritidis, and Y. enterocolitica [64]. SAEW with neutral pH value was effective for reducing the population of total aerobic bacteria (TAB), total viable counts (TVC), total coliforms mesophilic count, and even yeast and mold, while maintaining the properties of pork, chicken, and beef including increased shelf life, enhanced acceptable sensory properties, and delayed lipid oxidation [63,65]. Notes: ORP: Oxidation-reduction potential; NEW: neutralized electrolyzed water; SAEW: slightly acidic electrolyzed water; AEW: acidic electrolyzed water. ACC: available chlorine concentration.

Application of EW in Aquatic Product
Aquatic products contain high quality proteins, which are easily polluted by spoilage bacteria or pathogenic from the process of production, transportation to sale [74]. These bacteria not only reduce seafood quality and shorten shelf life but also pose a potential risk to human health. Currently, EW technology has been used for safety control and preservation of aquatic products, and these are shown in the Table 3. Hamzah et al. demonstrated that AEW ice treatment exhibited visible bacterial reduction on mussel and clam for 1 h [75]. Xuan et al. studied that SAEW ice could control microbial safety and quality deterioration of squid [76]. SAEW ice treatment could delay browning and softening appearances, maintain lower thiobarbituric acid (TBA) and total volatile basic nitrogen (TVEN) content, and inhibit the incensement of peroxide value. Furthermore, Zhao et al. investigated the changes of key proteins on shrimps during AEW ice storage [77]. The results showed that AEW ice significantly retarded the degradation of myofibrillar proteins in shrimps, including actin, myosin, and tropomyosin. The combination of EW with other technology in aquatic products is discussed in the hurdle technology section in more detail.

Hurdle Enhancement of EW with Other Treatments
EW is an effective broad-spectrum antimicrobial agent with several advantages: on the spot, cheap, environmentally friendly, and safety production [81]. However, it is necessary to face and solve the problem that EW is still unstable and insufficient for completely inactivating or decontaminating many food or other products [82]. In addition, the demand for organoleptic quality and fresh and nutritious food requires the development of novel technologies for prolonging shelf life. Currently, hurdle technologies combing two or more preservative and sanitizing technologies can improve inactivation efficacy without undesirable effects on food quality [83]. EW combined with other technologies has been widely studied for more than 15 years. Hurdle enhancement of EW has attracted much attention due to its synergistic microbial effectiveness on targeted microorganisms. Many studies have evaluated a significant reduction in microbial loads in various food products after treatment with a combination of EW and chemical treatments. EW, as a combination, has been used in different ways, including simultaneous hurdles and sequential hurdles, and is shown in Table 4.  However, a practical synergistic effect was observed when EW is combined with organic acids (citric acid [CA], fumaric acid [FA], and lactic acid), essential oils, benzalkonium chloride, etc., when simultaneously used against foodborne pathogens on food products. Rahman et al. investigated the combined sanitizing effects of AkEW with 1% CA at 50 • C on fresh cabbage [92]. Their results revealed a significantly reduced effective reduction in E. coli O157:H7 (4.19 log CFU/g) and L. monocytogenes (3.99 log CFU/g). Tango et al. found that CaO washing tomato and apple followed by SAEW + FA showed effective decontamination. Moreover, the softened skin of tomato fruits can be maintained by this method due to the inclusion of calcium [105]. In addition to EW, organic acids also significantly influence disinfection power. It is well known that organic acids have great potential for controlling a wide range of microorganisms. The main reason for the antimicrobial effect is the lipophilic nature of the undissociated form of organic acids and proton modification.
Ahmad et al. also studied that SAEW combined with 0.5% FA on pork showed a more significant bactericidal effect and prolonged shelf life at 6 days [106,107]. Consequently, bacterial viability decreased because essential enzymes and purine bases are negatively affected [108]. Hence, the synergistic effect of EW and organic acid could eliminate or inhibit brand microbes, organoleptic quality enhancement, and shelf-life prolongation by using different mechanisms [86,109].
Treatment with the combination of EW and physical sanitizing techniques has also been studied to guarantee microbiological safety and quality of the final product. For example, ultraviolet irradiation, ultrasound, heat treatment, sonication, and multi pulsed, high pressure have been successfully applied to subsequent treatment with EW against foodborne pathogens. Wu et al. reported that low concentrations of AEW combined with ultrasound (US) had a better bactericidal effect on mushrooms than individual methods [39]. Recently, Zahra et al. studied the effect of AEW/ozone combination against Salmonella on the quality of alfalfa seeds and sprouts. Their results showed a 3.6 and 2.9 log CFU/g reduction of seeds and sprouts, respectively, while no significant changes in the quality were observed, including shelf life, weight, and color.
Currently, Jiang et al. also studied the effect of the ultraviolet light-emitting diode in combination with SAEW against Salmonella and E. coli O157:H7 on the surfaces of coriander. The results revealed that the above method resulted in a 2.72 log CFU/g Salmonella reduction and 2.42 log CFU/g E. coli reduction and did not adversely affect the quality and increased shelf life [95]. The treatment SAEW combined with ultraviolet (UV) radiation also improves internal egg quality during six weeks of storage [110].
Furthermore, the use of EW combined with chemical and physical treatments to improve antimicrobial activity has been studied by several researchers. When the combination of benzalkonium chloride (BAC) and SAEW at 60 • C was investigated on vegetative cells, spores, and biofilms, a synergistic antimicrobial effect was observed, which resulted in reductions of ATCC 10,987 biofilms, ATCC 14,579 spores, and ATCC 10,987 spores at 2.62 log CFU/cm 2 , 1.98 log CFU/mL, and 1.91 log CFU/mL, respectively [87]. EW-based hurdles overcome certain limitations of individual treatment and maintained maximum food quality and safety attributes. These novel hurdle inactivation approaches could be an effective strategy against pathogens in food products, food contact surfaces, and medical instruments.

Clinical Application of EW in Hospitals
The demand for sanitizers and disinfectants in the health care and medical sectors has increased worldwide since the outbreak of COVID-19. The U.S. Environmental Protection Agency has recommended many disinfectants against COVID-19, including HOCl. In addition, owing to EW with a pH-neutral, safe, and broad-spectrum bactericide, the Ministry of Health of the People's Republic of China released three Chinese standards for materials and restricted substances in disinfectants, general requirements for hand disinfectants in April 2020, and general requirements for the disinfectants of mucous membrane [14]. EW can be used to disinfect medical instruments, clinical environments, and object surfaces and it can be used for disinfecting hands, skin, and mucous membranes, as shown in the Table 5. HOCl is the approved core formula, which has been incorporated into topical formulations due to its antimicrobial, immunomodulatory, anti-inflammatory, and wound healing properties. Currently, there are a variety of disinfection products based on EW in the market. In addition, its pH neutralization can enhance skin tolerability, therapeutic activity, and stability [115]. Many patents, including EW application for dental care, eye care, advanced tissue care, hand sanitizer, and dermatology, are available. EW with an antimicrobial effect has been used as a part of cell proliferation, anti-biofilm, and antiinfection therapies in a wound-healing agent [115]. Ben et al. discovered that, with the application of MicroSafe ® as an installation fluid with novel foam dressing and adverse pressure wound treatment for the patient, the wound showed significant improvement after three days of therapy [106,107]. In addition, HOCl has been reported to have antiinflammation and anti-itch effects on patients with atopic dermatitis [113]. Tomoki et al.
reported that NC/Nga mice reduced scratching and lesions behavior and inflammatory cytokines (IL-12) after treatment of HOCl gel.
Microorganisms related to hand hygiene and healthcare include E. coli, Pseudomonas aeruginosa, S. aureus, Enterococcus hirae, and Candida albicans. Sipahi et al. found that StAEW was significantly effective against test microorganism (E. hirae, C. albicans, and P. aeruginosa) populations, which all decreased by 100% in 1 min treatment [116]. Available chlorine concentration (ACC), used explicitly for hand sanitizers, is effective at 30 ppm to 150 ppm. SAEW may be a new clinical disinfectant that might be considered as a substitute for traditional alcohol-based hand sanitizer. Hsieh reported that electrolyzed oxidizing water (HOCl: 0.0125%) showed >99.9% antimicrobial effectiveness against Streptococcus mutans in 0.5 min treatment with little toxicity [114]. There is a possibility for electrolyzed water to be an alternative mouthwash for dental care and oral hygiene.

Role of EW in Functional Food Development
There is growing demand worldwide to produce and consume low-risk fresh food such as germinated food. Germination has widely been used to produce sprouts and young edible seeds for daily consumption. It can provide improved nutritional value, which includes free amino acids, vitamins, minerals, dietary fiber, polyphenols, and bioactive compounds, through simple germination procedures without long-term growth [23]. Seed sprouts, such as broccoli, buckwheat, radish, alfalfa, mung bean, and pea seeds, are commonly consumed raw in sandwiches and salads due to their nutritional value [117]. However, seed sprouts could easily be contaminated with foodborne pathogens, particularly E. coli O157:H7 and Salmonella, and other Shiga-toxin producing E. coli, L. monocytogenes, and B. cereus [118]. EW has become an acclaimed alternative for harsh chemical sanitizers in sprout growth and final products due to its substantial bactericidal effects, environmentally friendly attributes, and being generally recognized as having a safe (GRAS) status [22]. In general, the effect of EW applied in sprouts can be divided into three parts: inhibition of microbial growth, accumulation of functional components, and effect on morphological characteristics of sprouts.
Microbial contamination can be easily found in the process of germination due to its healthy, moist, and warm environment. In order to control the outbreak of foodborne diseases caused by a variety of pathogens in sprouts, researchers have recently found EW as a chemically based seed disinfection technique. As shown in Table 6, the total (aerobic) bacteria, molds, and yeasts on sprouts in the soaking or spraying treatment of EW after about one week's germination was generally a 1-3 log reduction compared to tap water treatment. In addition, EW could reduce the population of inoculated pathogens on vegetable sprouts to an acceptable level, such as E. coli O157:H7, L. monocytogenes, and S. enteritidis [119,120]. Furthermore, researchers reported that EW with increasing ACC used in seed soaking and pre-germination resulted in more significant reductions in Enterobacteriaceae populations [22]. SAEW with near-neutral pH (5-6.5) had excellent antibacterial effect. SAEW is more widely used in food disinfection. SAEW has been considered a useful method for eliminating pathogens with little effect on seed germination [22,44]. Meanwhile, a few authors emphasized the importance of pH in the germination process. The pH of EW can not only affect and differentiate the form of ACC, which would change antibacterial efficacy, but also affect the morphological characteristics of the sprouts. Notes: AEW: acidic electrolyzed water; SAEW: slightly acidic electrolyzed water; EW: electrolyzed water. ACC: available chlorine concentration.
Sprouts' growth conditions could be directly reflected by their morphological characteristics of germination rate, hundred-grain weight, sprouts length, hypocotyl length, and hypocotyl diameter. Spraying them with EW during germination has different effects on these indexes, varying with different seed species. Specifically, SAEW could promote the growth of mung bean sprouts and brown rice [131,133]. However, Han reported no significant difference between SAEW treatment and control in the above-mentioned parameters [122].
Sprouts have been recognized as wellness and health-promoting foods due to their high nutrient content and bioactive compounds, including vitamins, minerals, phenolic, carotenoids, and γ-aminobutyric acid (GABA) [134][135][136]. The synthesis of such second metabolites often occurs when plants are stressed by various elicitors or signal molecules such as EW. Secondary metabolites play an important role in overcoming stress conditions and adapting plants to the environment [137]. SAEW has been considered a valuable means for increasing the accumulation of GABA in the millet, which increased the content of GABA higher 21% than tap water [130]. Hao reported that buckwheat treated with EW (ACC: 10.94 ppm) increased total phenolics, total flavonoids, and rutin content on the ninth day showing 63.0%, 113.07%, and 128.1% increases with respect to tap water [121]. In addition, Li reported that a significantly higher content of sulforaphane (95%) in broccoli sprouts was revealed when SAEW (40.8 ppm) was exerted, which is 1.95 times as much as tap water [126].

Future Trends on the Application of EW
As a novel promising sanitizer and cleaner, EW has become popular in the food industry in recent years. EW is widely used in various fields, including agriculture, food sanitation, livestock management, medical disinfection, clinical, and other fields using antibacterial technology. EW has been approved by Russia, Japan, and China as a sanitizing agent in the food field. Recently, the commercialization of EW generators continues to grow around the globe. Given the importance of EW, many companies have designed and produced EW generators. Examples of such companies include Eco Tree, Emcao Tech Athena, TECH Corporation, Jing Jun, and RVD Corporation, which are based in China, Japan, South Korea, and U.S.A. The companies are making electrolyzed water generators used in research laboratories, and commercialization for convenient household usage is encouraged by these companies. More importantly, with the development of portable EW generators, EW can be used for households (spray form) in the next step.
The United States Department of Agriculture National Organic Program approved HOCl generated by electrolyzed water as an organic-compatible sanitizer. Furthermore, EW can disinfect medical instruments, clinical environments, and object surfaces and is used for disinfecting hands, skin, and mucous membranes in China. The demand for EW products is driving many companies to search for new locations to setup facilities for manufacture, including Microcyn ® , Clortech ® , Avenova ® , Ecasol™, and MicroSafe ® . Industries claim to generate EW-based products that are safe to use around the nose, mouth, and eyes while having a remarkable antimicrobial effect. Due to the high impact that it could have in breaking the chain of infection in the dental office, EW could be used in the pre-procedural stage of oral rinsing as a pre-procedural oral rinse, which has the potential to dramatically reduce the quantity of viruses, along with preventative measures and further application as personal protective equipment. Recently, in order to further improve the antibacterial activity of EW, hurdle technology, combined with two or more scaffold techniques, has been developed and it shows synergistic effects in terms of decreasing microbial population, prolonging product's shelf life, and maintaining product's quality), integrating disinfection technologies and disinfection mechanisms of EW to develop a hurdle method with synergistic effects for further improving the antimicrobial effect. The strategy will continue depending on production and manipulation, including using powerful tools (flow cytometer) and molecular biology (including transcriptomics, proteomics, metabolomics, and other omics) to fully understand the sterilization mechanism. An advanced and dynamic EW production system that is capable of overcoming all the current limitations can be overcome by conducting further research in the field.
Based on recent updates, it is believed that EW may be applied in the food industry as a possible disinfectant. EW has been certified by U.S.A. government regulators as a safer alternative to hazardous chemicals when applied at home, industry, and institutions, and they are mainly green and sustainable. Despite the application of EW in various sectors in Russia and Japan for several decades, its affirmation in the U.S.A. and other countries is slowly growing. Recent observations showed that there is widespread commercialization of EW around the globe. Several industries have emerged due to the importance of electrolysis water and due to rising demand. Examples of the type of company include Envirolyte, KEWS, RVD Corporation, EcoLogic Solutions Inc., and Viking pure, which are based in Asia (China, South Korea, and Japan), Europe (Latvia), and the United States.
The major advantages of EW in the growing industrial sector include the manufacture of 100% organic, non-toxic, and low-cost disinfectants for both household and industrial applications. A US-based company (EAU Technologies Inc.) completed a successful field trial in 2009 by using electrolysis water technology for Clean-in-Place (CIP) application fields, which can be applied by using Empowered Water, a brand-name for electrolysis water made by Electric Aquagenics Unlimited (EAU) Technologies. Specifically, in the industrial food sector, CIP implementations and framework enterprises clean equipment on stationary platforms (EAU 2009). Globally, depending on the location, people have differing views on sanitizing applications and electrolysis water regulations. Due to the effects of amino acid inactivation, European Union members may only apply electrolysis water to drinking water, and it is not approved for use in food products such as meat and fish. However, in the United States, it is typically applied for sanitization and disinfecting agents. United States Department of Agriculture has recently approved electrolysis to fresh produce (organic) products. Although it is simple in formulation, EW solution loses its effectiveness once it becomes exposed to water. Even though the eyes are exposed to water, humans are not affected by exposure to electrolysis as long as it is not ingested orally. The expertise of electrolysis is not widespread in the chemical industry; thus, additional exposure to this topic is required. EW is likely to be used in food plants over the next decade due to its advantages.

Conclusions
The application of EW has efficient antimicrobial with bactericidal effects on fruits, vegetables, fish, aquatic, poultry, utensils, and raw meat in various industries. However, EW with high acidity (pH 2.2-2.7) and corrosive nature alters the organoleptic properties of specific foods, which limits its use. Some of these concerns have been addressed, and they alter the properties of electrolysis water, such as SAEW with pH (5.0-6.5). In terms of efficient microbial reduction with increasing shelf life and maintaining the nutritional and organoleptic properties of food, this can be achieved with hurdle technology (combination of EW with sonication, organic acid, and heat treatment), which overcomes disadvantage in the EW treatment process. Furthermore, the efficiency of EW depends on storage temperature, ACC and ORP, hydrogen ion concentration (pH), salt concentration, and other physiochemical properties of EW. Furthermore, the amount of organic matter and water hardness influences its bactericidal action.
As a result, further research in this specific sector has to be improved in order to overcome limitations in the dynamic EW production system. These may include procedures for increasing the application of EW in food processing settings and further use in HACCP and Sanitation Standard Operating Procedures (SSOPs). Nowadays, SAEW has no irritation in human application and no safety issues from Cl 2 off-gassing. The future of EW technology application in clinical applications appears to be very promising, especially with respect to hands, skin, and mucous membranes.