Bioassay-Guided Discovery of Potential Partial Extracts with Cytotoxic Effects on Liver Cancer Cells from Vietnamese Medicinal Herbs

: Hepatocellular carcinoma (HCC) is the most frequent type of primary liver cancer and is the leading cause of cancer mortality in Vietnam. Our study aims to discover the partial extracts with the potential cytotoxic effects on HCC cells from the different parts of 24 Vietnamese medicinal plants traditionally used in liver cancer treatment. Out of 52 crude methanol extracts, we found that Luvunga scandens leaves, Hyptis suaveolens roots, and Solanum torvum leaves showed the notable cytotoxic effects against HCC cells. After that, we carried out partial extract of the three methanol extracts with ethyl acetate, water, n- hexane, and 90% methanol. The cytotoxic activity on Huh-7 HCC cells, antioxidant capacity, and total ﬂavonoids content (TFC) of each partial extraction were determined. Methanol, ethyl acetate, and 90% methanol extracts showed moderate to strong cytotoxicity activity against Huh-7 HCC cells. Notably, the ethyl acetate and 90% methanol extract from H. suaveolens roots with high TFC values and strong antioxidant capacity could be promising sources of novel therapeutic modalities for HCC treatment. For the leaves of L. scandens and S. torvum , the ethyl acetate extract showed high TFC value and promising anti-HCC activity, therefore recommended further studies.


Introduction
Liver cancer has become a warning problem with 905,677 new cases, 830,180 new deaths [1], and was the leading cause of cancer-related death in Vietnam recorded in 2020 [2]. The main two forms of primary liver cancer are hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma [3]. HCC is the most common type of primary liver cancer, comprising 75-85% of cases [1]. The HCC prognosis is frequently poor as most HCC patients are detected at the late stage of liver cancer; thus, the mortality rate is exceptionally high and approximately equal to the incidence rate [4][5][6].
There are several treatment options for HCC patients based on the stage of HCC. For early-stage of HCC, surgical resection is suggested because of safety and effectiveness, even in elderly patients [7,8]. As well as surgical resection, chemotherapy is also regularly performed in the HCC treatment. However, further problems from using chemotherapy drugs such as toxicity, drug inefficacy, and some severe side effects for long-time treatment led to insignificant effectiveness in improving outcomes [9]. An unconventional and long-term method to treat chronic diseases, including HCC, is the use of medicinal herbs because of a certain number of benefits such as high bioactivity, less unexpected effects, and less toxicity to normal cells [10,11]. There are 62 compounds among 247 anticancer drugs approved from January 1981 to September 2019 that are natural products or derived from a natural product [12]. In addition, more than half of the medicines approved by the U.S. Food and Drug Administration for the treatment of cancer are natural products or plant-derived natural products [13]. Consequently, the use of biologically active substances from medicinal herbs in supporting the treatment and prevention of cancer is a trend that deserves attention.
Vietnam has plentiful medicinal plant resources with more than 4700 species used as a medicine, which is advantageous for screening herbals acting on HCC cells [14]. For a long time, traditional herbal remedies were used for the treatment of malignant and chronic diseases, including ascites, cirrhosis, and liver diseases [14][15][16][17]. However, the current use of medicinal plants in Vietnam is based chiefly on folk experience or traditional documents such as Medicinal plants in An Giang Province (1991) [15], Plants and animals used as medicine in Vietnam (2004) [16], Vietnamese Medicinal Plants and Remedies (2004) [17], and Dictionary of Vietnamese Medicinal Plants (2012) [14]. The most significant advantage of screening the truly beneficial herbs is improving treatment effect and saving time as well as money for patients. Additionally, the screening investigation also allows researchers to discover new bioactive natural products. Therefore, studying and expanding the screening experiments for nations with plenty of medicinal plants like Vietnam is essential. Typically, when plant extraction was shown to be biologically active, a bioassay-guided partial extraction was carried out to find the potential extract for isolation, identification compounds, and drugs development [12,18].
In the present study, we conducted a cytotoxic screening against three liver cancer cell lines Huh-7, Hep3B, and HepG2, with 52 crude methanol extracts prepared from different parts of 24 medicinal herbs. These medicinal plants were recommended by the Tinh Bien District Orientally Traditional Medicine Association, An Giang Province, Vietnam based on folk experience, traditional remedies, and the Vietnamese traditional documents for the treatment of liver cancer and ascites for a long time [14][15][16][17]. Three hit crude extracts from Luvunga scandens leaves, Hyptis suaveolens roots, and Solanum torvum leaves were selected for partial extraction to yield four additional partial extracts from each crude extract. Here, the total flavonoids content (TFC), the antioxidant activity, and the potency of cytotoxic effect against Huh-7 HCC cells of these extracts were determined.

Collecting, Preparing, and Extracting Plant Materials
All plant materials were collected in Tinh Bien District, An Giang Province, Vietnam in October 2020 and authenticated by Dr. Hoang Viet, Department of Ecology-Evolutionary Biology, University of Science, Vietnam National University Ho Chi Minh City (Table 1). These fresh samples were washed, drained, cut into small pieces, and completely dried at 50 • C before being ground and sieved using a strainer with 1 mm mesh size pore. Each plant powder (20.0 g) was soaked in methanol (160 mL) for 3 days, filtered with Newstar 102 filter paper, and the solvent was removed using a vacuum evaporator to yield the crude methanol extract.

Cell Cultures
Huh-7, Hep3B, and HepG2 HCC cell lines were kindly provided by Professor Yan-Hwa Wu Lee (Institute of Biochemistry and Molecular Biology, School of Life Sciences, National Yang-Ming University). All three cell lines were cultured in complete DMEM. The DMEM medium included 10% fetal bovine serum (Gibco), penicillin (100 U/mL), streptomycin (100 µg/mL), nonessential amino acids (0.1 mM), and L-glutamine (2 mM). The cells were maintained at 37 • C and an atmosphere of 5% CO 2 .

Cytotoxicity Screening ASSAY
The crude methanol extracts were screened for cytotoxicity effect on Huh-7, Hep3B, and HepG2 HCC cell lines by using MTT assay. Briefly, the cells (1 × 10 4 ) were seeded in a 96-well plate with the cell culture medium (100 µL) and treated with the plant extracts for 48 h. The concentrations of plant extracts were 100 µg/mL. DMSO at a concentration of 1% was used as a control group. After incubation, the cell cultures were changed to MTT solution with the final concentration was 0.5 mg/mL and continue incubated for 4 h. Formazan crystals were dissolved in DMSO, and the optical density was measured at 550 nm using the Microplate Reader Thermo Multiskan Ascent [19,20].
The cell viability was calculated using the equation: The experiment was performed in three times and all data are presented as mean ± SD.

Preparation of Partial Extracts
The crude methanol extracts of designated medicinal herbs were partitioned by a two-phase solvent system ethyl acetate: water (4:3, v/v), with solid: liquid ratio (1:4, g/L) in the separating funnel. Shook vigorously and allowed for the solvent layer to separate completely for 1 min. After phase separation, the water layer was then collected, followed by the partial extraction process, which was repeated four times with the same ratio to obtain ethyl acetate layer and water layer separately. All ethyl acetate layers and water layers were combined, respectively, and then the solvents were removed using a vacuum evaporator to obtain ethyl acetate extract and water extract. Ethyl acetate extract was then partitioned in a two-phase solvent system consisting of n-hexane: 90% methanol (1:1, v/v). This step was performed the same as the first partition step to yield n-hexane phase and 90% methanol phase. The solvent removal was done by using a vacuum evaporator to obtain n-hexane extract and 90% methanol extract. The partial extracts were then stored at 4 • C. The partial extraction process is shown in Figure 1. obtain n-hexane extract and 90% methanol extract. The partial extracts were then stored at 4 °C. The partial extraction process is shown in Figure 1.

Total Flavonoids Content (TFC)
Total flavonoids content was determined by using the aluminum-flavonoid complex colorimetric method [21,22]. Exact amounts (0.2, 0.4, 0.8, 1.2, 1.6, 2.0 mL) of the standard solution of rutin (250 μg/mL) were added into 10 mL volumetric flasks. The standard solutions were then mixed with sodium nitrite solution (5%, 0.3 mL). After 5 min, aluminum chloride solution (2%, 0.3 mL) was added, rested for 6 min, and mixed with sodium hydroxide solution (1 M, 3.0 mL). The volume was adjusted to 10 mL with diluted water and allowed to stand at room temperature for 10 min. The amount of aluminum chloride solution (2%) was substituted with the same amount of water in the blank. The absorbance was measured at 510 nm against the blank using Hitachi U-3900 Spectrophotometer. All analyses were performed in triplicate.
The same procedure was applied for all crude extracts and partial extracts. TFC was calculated as rutin equivalents per gram of dry weight of extract (mg RE/g extract) based on the formula: In which: TFC: total flavonoids content (mg RE/g extract). a: value from the calibration curve of rutin standard (μg/mL). V: volume of total solution (L). m: mass extract in V (g).

DPPH Free Radical Scavenging Assay
The antioxidant activity was evaluated following a previously described DPPH free radical scavenging capacity method [23]. DPPH can produce stable free radicals in methanol solution with specific purple color and react with antioxidants yielding a yellow complex. In brief, a serial dilution (the concentration range between 5 and 650 μg/mL) of the extract were mixed with a methanol solution of DPPH (0.2 mM, 1.1 mL) and adjusted the volume to 3 mL with methanol. The mixtures were stored in the dark at room temperature for 15 min. The control sample was prepared at the same conditions without the extracts. After that, the absorbance of the samples was measured at 516 nm corresponding to the maximum absorbance peak of DPPH using the Shimadzu UV-1800 UV/Visible Scanning Spectrophotometer with methanol as a blank. Each experiment was performed in triplicate.
The percentage of DPPH radical scavenging was estimated using the equation:

Total Flavonoids Content (TFC)
Total flavonoids content was determined by using the aluminum-flavonoid complex colorimetric method [21,22]. Exact amounts (0.2, 0.4, 0.8, 1.2, 1.6, 2.0 mL) of the standard solution of rutin (250 µg/mL) were added into 10 mL volumetric flasks. The standard solutions were then mixed with sodium nitrite solution (5%, 0.3 mL). After 5 min, aluminum chloride solution (2%, 0.3 mL) was added, rested for 6 min, and mixed with sodium hydroxide solution (1 M, 3.0 mL). The volume was adjusted to 10 mL with diluted water and allowed to stand at room temperature for 10 min. The amount of aluminum chloride solution (2%) was substituted with the same amount of water in the blank. The absorbance was measured at 510 nm against the blank using Hitachi U-3900 Spectrophotometer. All analyses were performed in triplicate.
The same procedure was applied for all crude extracts and partial extracts. TFC was calculated as rutin equivalents per gram of dry weight of extract (mg RE/g extract) based on the formula: In which: TFC: total flavonoids content (mg RE/g extract). a: value from the calibration curve of rutin standard (µg/mL). V: volume of total solution (L). m: mass extract in V (g).

DPPH Free Radical Scavenging Assay
The antioxidant activity was evaluated following a previously described DPPH free radical scavenging capacity method [23]. DPPH can produce stable free radicals in methanol solution with specific purple color and react with antioxidants yielding a yellow complex. In brief, a serial dilution (the concentration range between 5 and 650 µg/mL) of the extract were mixed with a methanol solution of DPPH (0.2 mM, 1.1 mL) and adjusted the volume to 3 mL with methanol. The mixtures were stored in the dark at room temperature for 15 min. The control sample was prepared at the same conditions without the extracts. After that, the absorbance of the samples was measured at 516 nm corresponding to the maximum absorbance peak of DPPH using the Shimadzu UV-1800 UV/Visible Scanning Spectrophotometer with methanol as a blank. Each experiment was performed in triplicate.
The percentage of DPPH radical scavenging was estimated using the equation: In which: SC%: percentage of DPPH free radical scavenging effect. As: absorbance of the standard.
Processes 2021, 9, 1956 5 of 14 Ae: absorbance of the extractives. The half maximal inhibitory concentration (IC 50 ) value of the extracts was calculated at the value that inhibits 50% free radical DPPH and is calculated by interpolation.

Determination of 50% Cytotoxic Concentration (CC 50 )
The Huh-7 HCC cells were cultured in complete DMEM as described by Li et al. [24]. The Huh-7 HCC cells were seeded in a 96-well plate with cell culture medium (100 µL) and treated with the plant extracts for 72 h. The concentrations of plant extracts were ranged from 6.25-100 µg/mL. Resazurin solution was added and incubated for an additional 4 h at 37 • C and 5% CO 2 . After incubating, resazurin fluorescence (ex/em: 530 nm/590 nm) was measured from the culture supernatant using a Synergy HT Multi-Mode Reader to determine cell viability. The % cell viability values were plotted and used to determine CC 50 . Each experiment was repeated in triplicate.

Statistical Analysis
Statistical analysis was performed using Microsoft Excel 2016. The results of CC 50 were processed using GraphPad Prism 9 statistical software. All data were presented as means ± SD for three replications for each experiment.

Cytotoxicity Screening
The results of cytotoxicity screening on the three HCC cell lines with 52 extracts prepared from different parts (roots, leaves, stems, fruits, and rhizomes) of 24 medicinal herbs were presented in Table 1. Cytotoxicity effect values (%) showed the cytotoxicity of crude methanol extracts at a concentration of 100 µg/mL; the higher the value, the more cytotoxic activity. Most of the crude extracts did not cause significant cytotoxicity to these three cells. torvum leaves) exhibited cytotoxicity higher than 50% against at least one HCC cell line and were selected for further studies. Among them, the extracts of H. suaveolens roots were found to be the most potent extract that induced 73%~85% cytotoxicity in all three HCC cell lines. The extracts of L. scandens leaves cause 54% and 73% cytotoxicity in Hep3B and HepG2 HCC cells, respectively. The extract of S. torvum leaves showed notable cytotoxicity only to Hep3B HCC cells with a cytotoxic effect of around 77%.

Evaluation of TFC, Antioxidant Activity, and CC 50 of the Extracts from Luvunga scandens Leaves
The total flavonoids content of the extracts from L. scandens leaves are shown in Figure 2a and Tables S1-S3. The flavonoid compounds were enriched in ethyl acetate layer (233.46 ± 17.34 mg RE/g extract), and in n-hexane layer (354.89 ± 32.55 mg RE/g extract). As presented in Figure 2b, the extracts from leaves of L. scandens did not show antioxidant activity with IC 50 values > 250 µg/mL [49]. The methanol, ethyl acetate, and 90% methanol extracts from L. scandens leaves exhibited moderate cytotoxicity activity with CC 50 36.5 ± 2.9, 38.5 ± 2.1, 51.2 ± 1.5 µg/mL, respectively.

Evaluation of TFC, Antioxidant Activity, and CC 50 of the Extracts from Hyptis suaveolens Roots
The total flavonoids content, antioxidant activity, and cytotoxic effect of the extracts from H. suaveolens roots were described in Figure 3 and Tables S1-S3, respectively.
The total flavonoids content of H. suaveolens roots was highest in the ethyl acetate extract with TFC value was 312.23 ± 22.82 mg RE/g extract, followed by those of 90% methanol extract and water extract with 259.75 ± 6.96 mg RE/g extract and 249.67 ± 23.71 mg RE/g extract, respectively (Figure 3a, Table S1).
The total flavonoids content of the extracts from L. scandens leaves are shown in Figure 2a and Tables S1-S3. The flavonoid compounds were enriched in ethyl acetate layer (233.46 ± 17.34 mg RE/g extract), and in n-hexane layer (354.89 ± 32.55 mg RE/g extract). As presented in Figure 2b, the extracts from leaves of L. scandens did not show antioxidant activity with IC50 values > 250 μg/mL [49]. The methanol, ethyl acetate, and 90% methanol extracts from L. scandens leaves exhibited moderate cytotoxicity activity with CC50 36.5 ± 2.9, 38.5 ± 2.1, 51.2 ± 1.5 μg/mL, respectively.

Evaluation of TFC, Antioxidant Activity, and CC50 of the Extracts from Hyptis suaveolens Roots
The total flavonoids content, antioxidant activity, and cytotoxic effect of the extracts from H. suaveolens roots were described in Figure 3 and Tables S1-S3, respectively.
The total flavonoids content of H. suaveolens roots was highest in the ethyl acetate extract with TFC value was 312.23 ± 22.82 mg RE/g extract, followed by those of 90% methanol extract and water extract with 259.75 ± 6.96 mg RE/g extract and 249.67 ± 23.71 mg RE/g extract, respectively (Figure 3a, Table S1).

Evaluation of TFC, Antioxidant Activity, and CC50 of the Extracts from Solanum torvum Leaves
The total flavonoids content, the antioxidant activity, and the cytotoxicity effect of the extracts from S. torvum leaves were evaluated and designated in Figure 4 and Tables S1-S3. The highest TFC value was found in n-hexane extract (230.64 ± 16.74 mg RE/g extract).
The scavenging effects of all the extracts on DPPH radicals did not show significant antioxidant capacity with IC50 values > 250 μg/mL, and the 90% methanol extract was not determined scavenging activity hidden in Figure 4b [49].
Results from the cytotoxicity assay of S. torvum leaves showed a strong cytotoxicity effect with the CC50 was 24.9 ± 6.9 μg/mL in ethyl acetate extract as well as moderate cytotoxicity effect in the case of the methanol and 90% methanol extracts (53 ± 2.2, 61.1 ± 2.0 Except for n-hexane extract, the remaining extracts from H. suaveolens roots exhibited strong radical scavenging activity [49]. The 90% methanol extract was the most potent extract with IC 50 of 13.27 ± 0.68 µg/mL (Figure 3b, Table S2).

Evaluation of TFC, Antioxidant Activity, and CC 50 of the Extracts from Solanum torvum Leaves
The total flavonoids content, the antioxidant activity, and the cytotoxicity effect of the extracts from S. torvum leaves were evaluated and designated in Figure 4 and Tables S1-S3. The highest TFC value was found in n-hexane extract (230.64 ± 16.74 mg RE/g extract).

Total Flavonoids Content, Antioxidant Activity, and Cytotoxic Effect of the Extracts from Luvunga scandens Leaves
The study by Permanasari et al. reported that the n-hexane and dichloromethane extract from L. scandens leaves at a concentration of 30 µ g/mL showed the ability to inhibit 97.88 ± 0.80%, and 83.97 ± 1.02% the growth Huh-7 HCC cells, respectively [50]. In this study, the n-hexane extract of L. scandens leaves did not show cytotoxic effect, while the ethyl acetate extract and methanol extract showed moderate cytotoxicity ability with CC50 values were 36.5 ± 2.9 and 38.5 ± 2.1 µ g/mL, respectively. The high TFC value of ethyl acetate extract (233.46 ± 17.34 mg RE/g extract) could be due to the partial extraction procedure by which the compounds having cytotoxic effects were more concentrated in the ethyl acetate extract.

Total Flavonoids Content, Antioxidant Activity, and Cytotoxic Effect of the Extracts from Hyptis suaveolens Roots
The extracts from H. suaveolens roots exhibited the superior cytotoxic potential in Huh-7 HCC cells with the CC50 value ranging from 1.6 to 14.4 µ g/mg. Although the roots of H. suaveolens have been used as Vietnamese traditional medicine plants to treat liver cancer for years, the cytotoxic effect on HCC cell lines has never been reported. Nevertheless, numerous cytotoxic activity studies on the H. suaveolens extracts against other cancer cell lines have been published. Indeed, the ethanol extract from H. suaveolens twigs, leaves, and flowers had been studied for its strong anticancer potent in the KB cell culture and the P-388 lymphocytic leukemia system for a long time [51]. Gurunagarajan and Pemaiah reported that the ethanol extract from the aerial parts of H. suaveolens had strong activity against Ehrlich Ascites carcinoma cell lines with the CC50 was of 10.63 μg/mL [52]. In addition, H. suaveolens leaves were proposed to protect the liver against CCl4-induced oxidative damage in rats. Furthermore, the water extract from H. suaveolens leaves was also reported to have hepatoprotective potentials on acetaminophen-induced liver damage in The scavenging effects of all the extracts on DPPH radicals did not show significant antioxidant capacity with IC 50 values > 250 µg/mL, and the 90% methanol extract was not determined scavenging activity hidden in Figure 4b [49].
Results from the cytotoxicity assay of S. torvum leaves showed a strong cytotoxicity effect with the CC 50 was 24.9 ± 6.9 µg/mL in ethyl acetate extract as well as moderate cytotoxicity effect in the case of the methanol and 90% methanol extracts (53 ± 2.2, 61.1 ± 2.0 µg/mL, respectively) on Huh-7 HCC cells.

Total Flavonoids Content, Antioxidant Activity, and Cytotoxic Effect of the Extracts from Luvunga scandens Leaves
The study by Permanasari et al. reported that the n-hexane and dichloromethane extract from L. scandens leaves at a concentration of 30 µg/mL showed the ability to inhibit 97.88 ± 0.80%, and 83.97 ± 1.02% the growth Huh-7 HCC cells, respectively [50]. In this study, the n-hexane extract of L. scandens leaves did not show cytotoxic effect, while the ethyl acetate extract and methanol extract showed moderate cytotoxicity ability with CC 50 values were 36.5 ± 2.9 and 38.5 ± 2.1 µg/mL, respectively. The high TFC value of ethyl acetate extract (233.46 ± 17.34 mg RE/g extract) could be due to the partial extraction procedure by which the compounds having cytotoxic effects were more concentrated in the ethyl acetate extract.

Total Flavonoids Content, Antioxidant Activity, and Cytotoxic Effect of the Extracts from Hyptis suaveolens Roots
The extracts from H. suaveolens roots exhibited the superior cytotoxic potential in Huh-7 HCC cells with the CC 50 value ranging from 1.6 to 14.4 µg/mg. Although the roots of H. suaveolens have been used as Vietnamese traditional medicine plants to treat liver cancer for years, the cytotoxic effect on HCC cell lines has never been reported. Nevertheless, numerous cytotoxic activity studies on the H. suaveolens extracts against other cancer cell lines have been published. Indeed, the ethanol extract from H. suaveolens twigs, leaves, and flowers had been studied for its strong anticancer potent in the KB cell culture and the P-388 lymphocytic leukemia system for a long time [51]. Gurunagarajan and Pemaiah reported that the ethanol extract from the aerial parts of H. suaveolens had strong activity against Ehrlich Ascites carcinoma cell lines with the CC 50 was of 10.63 µg/mL [52]. In addition, H. suaveolens leaves were proposed to protect the liver against CCl 4 -induced oxidative damage in rats. Furthermore, the water extract from H. suaveolens leaves was also reported to have hepatoprotective potentials on acetaminophen-induced liver damage in rabbits [53]. Our finding suggested for the first time that the roots of H. suaveolens also possess anti-HCC activities, and it is also worth evaluating its liver protective effect.
Among three herbal medicines in this study, the antioxidant activity of H. suaveolens root extracts, except for the n-hexane extract, were the most prominent with IC 50 values ranging from 12.59 to 29.67 µg/mL. In a previous study, the methanol extract from H. suaveolens leaves has been reported to have a strong antioxidant activity (IC 50 = 24.56 µg/mL) [54]. Another study on the extracts from H. suaveolens leaves in polar solvents supported that they had remarkable antioxidant activity; particularly, the 70:30 ethanol/water extract possessed a maximum antioxidant activity with IC 50 = 2.73 ± 0.005 µg/mL [55]. Until now, there have been no studies on evaluating the antioxidant capacity from the extract of H. suaveolens roots. In this study, the cytotoxic effect and antioxidant capacity of H. suaveolens roots are directly proportional with the high values of total flavonoids content in the ethyl acetate and 90% methanol extracts.

Total Flavonoids Content, Antioxidant Activity, and Cytotoxic Effect of the Extracts from Solanum torvum Leaves
Although the cytotoxicity effect screening results of 24 medicinal herbs did not show the notable cytotoxicity of the crude methanol extract from S. torvum leaves against HCC Huh-7 HCC cells (Table 1), the ethyl acetate partial extract from S. torvum leaves exhibited a potent cytotoxicity against Huh-7 HCC cells with CC 50 of 24.9 ± 6.9 µg/mL (Table S3). A previous study also reported that the ethyl acetate extract from S. torvum fruits displayed better cytotoxic capacity against MCF-7 cells than its methanol extract and n-hexane extract [56].
Results in our study showed that the partial extracts from S. torvum had no antioxidant activity. As shown in Figure 4a and Table S1, the TFC value of extracts from S. torvum leaves ranged from 23.13 to 247.38 mg RE/g extract, which are in good agreement with previous studies. Indeed, Waghulde et al. reported that the total flavonoids content of S. torvum ethanol extract and methanol extract were 85.26 ± 0.02 and 65.69 ± 0.02 mg RE/g extract, respectively [57]. Another study was carried out on S. torvum fruits using Soxhlet extraction with petroleum ether, chloroform, ethyl acetate, methanol, and water showed that the TFC value of ethyl acetate, methanol, and water extracts were 1.013, 31.043, and 13.064 mg RE/g extract, respectively while the extracts in petroleum ether and chloroform did not present flavonoid [58].

Conclusions
This study screened 52 crude extracts from different parts of 24 traditional medicinal herbs for liver cancer treatment in Vietnam with outstanding cytotoxic activity against three HCC cell lines. The crude methanol extracts of L. scandens leaves, H. suaveolens roots, and S. torvum leaves were identified with potent anti-HCC activity. Then, we obtained four partial extracts with different polarity solvents, including ethyl acetate, water, n-hexane, and 90% methanol, and investigated the total flavonoids content, free radical scavenging activity, and cytotoxicity effect of all extracts. The methanol, ethyl acetate, and 90% methanol extracts of H. suaveolens roots and the ethyl acetate extract of the leaves of L. scandens and S. torvum exhibited strong cytotoxicity against Huh-7 HCC cells. These extracts could be promising sources for the discovery of novel therapeutic modalities for HCC treatment. Further studies are needed to isolate, identify, and quantify the bioactive constituents responsible for the anti-HCC capacity in each recommended extract.
Author Contributions: All authors contributed to designing and conducting experiments, data analysis, and interpretation as well as drafting and revising the manuscript. All authors have read and agreed to the published version of the manuscript.