Technical Aspects of Biofuel Production from Di ﬀ erent Sources in Malaysia—A Review

: Due to the depletion of fossil fuels, biofuel production from renewable sources has gained interest. Malaysia, as a tropical country with huge resources, has a high potential to produce di ﬀ erent types of biofuels from renewable sources. In Malaysia, biofuels can be produced from various sources, such as lignocellulosic biomass, palm oil residues, and municipal wastes. Besides, biofuels are divided into two main categories, called liquid (bioethanol and biodiesel) and gaseous (biohydrogen and biogas). Malaysia agreed to reduce its greenhouse gas (GHG) emissions by 45% by 2030 as they signed the Paris agreement in 2016. Therefore, we reviewed the status and potential of Malaysia as one of the main biofuel producers in the world in recent years. The role of government and existing policies have been discussed to analyze the outlook of the biofuel industries in Malaysia.


Introduction
Recently, concerns about the depletion of conventional fuels (oil, natural gas, and coal), global warming, and the related environmental issues have drawn the government's, researchers', and policy-makers' attention to identifying new energy sources [1]. Consequently, biofuels could be considered as an alternative to reduce high dependence on diesel fuels [2]. Besides this, the over-utilization of conventional fuels and their side effects on the Earth has increased the demand for liquid fuels produced from biomass throughout the world. According to estimations, the bioenergy production would be increased 4.7 times (from 9.7 × 106 to 4.6 × 107 GJ/d) between 2016 and 2040. However, it is noteworthy to mention that the biofuel production process influences their environmental impact [3].
Conventional fuels accounted for 80% of the primary energy consumption throughout the world in 2019, out of which about 60% were consumed by the transport sector [4]. Biofuels are classified Consequently, the demand for biodiesel, biogas, and bioethanol has increased. Therefore, achieving balance in the market and meeting the growing demand needs more production [5].
Generally, biofuels are divided into four main categories based on their production sources. The first generation is food-based biofuels and has several advantages, such as low production cost and effective production methods, which result in lower CHG emissions. Hence, the main issue of this type of biofuel is competition with food, which is a critical problem for consideration [6].
The main feedstock for second-generation biofuels is biomass, which is classified into two groups: (1) agricultural residues, such as sweet sorghum, sugarcane bagasse, and straws; (2) forest residues, such as energy crops and woody plants [7,8].
Third-generation biofuels are called algae-based biofuels, with the advantage of higher productivities per unit of area than other biofuel types of crops [9]. Meanwhile, fourth-generation biofuel is named genetically modified algae, with advantages such as having no food-energy conflict, requiring no arable land, and having an easy conversion. It is in the early stages of development, as reported by Abdullah et al. [10]. Figure 1 shows the increasing worldwide trend of biofuel production, which will reach 25% by 2024 [11]. Asia accounts for half of the growth, in which China is the largest biofuel producer to ensure its energy security and improve air quality [12]. Brazil is the second largest producer; meanwhile. two-thirds of the total biofuel production will be provided by the United States and Brazil in 2024 [13].

Possible Biomass Sources for Biofuels Production in Malaysia
Tropical regions such as Association of Southeast Asian Nations (ASEAN) have large areas of natural arable land for biomass production, which could be used to produce biofuel. The ASEAN countries have promoted their national programs to increase their biofuel industry equipped with modern and advanced technologies [14], and [15]. The rate of energy demand has increased in Malaysia due to industrialization and the increasing population (32.4 million, with an annual growth rate of 1.4% in 2018), which could be a problem in the next 40 years because of fossil fuel resource depletion [16]. Moreover, still the transport sector consumed more than 70% of the total energy in Malaysia, which is petroleum-based diesel and gasoline fuels [17]. Besides this, Malaysia is a developing country that has planned and developed its biomass industry and is targeted to become a developed country by 2030 [18].
In the last three decades, Malaysia has become one of the most important poles of biofuel technology in the world due to its abundant natural sources (forests and agricultural fields cover 76% of its total land). In this regard, several strategies have been implemented by the Malaysian government [19].

Possible Biomass Sources for Biofuels Production in Malaysia
Tropical regions such as Association of Southeast Asian Nations (ASEAN) have large areas of natural arable land for biomass production, which could be used to produce biofuel. The ASEAN countries have promoted their national programs to increase their biofuel industry equipped with modern and advanced technologies [14], and [15]. The rate of energy demand has increased in Malaysia due to industrialization and the increasing population (32.4 million, with an annual growth rate of 1.4% in 2018), which could be a problem in the next 40 years because of fossil fuel resource depletion [16]. Moreover, still the transport sector consumed more than 70% of the total energy in Malaysia, which is petroleum-based diesel and gasoline fuels [17]. Besides this, Malaysia is a developing country that has planned and developed its biomass industry and is targeted to become a developed country by 2030 [18].
In the last three decades, Malaysia has become one of the most important poles of biofuel technology in the world due to its abundant natural sources (forests and agricultural fields cover 76% of its total land). In this regard, several strategies have been implemented by the Malaysian government [19].
On the other hand, there are many sources such as biomass waste in Malaysia which can be used as a suitable replacement for fossil fuels [20][21][22][23][24]. This country produces 168 million tons of biomass annually, including rice husks, timber, coconut trunk fibers, oil palm waste, and municipal wastes.
For instance, Malaysia, with more than 20 million tonnes of palm oil production, placed as the world's second-largest producer in 2019 [25], and is forecasted to produce 20.3 million tonnes in 2020 [26]. It noteworthy to mention that Palm Oil Mill Effluent (POME) is wastewater produced from the oil palm industry and has environmental side effects if discharged into the environment. It contains a high level of organic nutrients, which can be converted to useful products such as biofuel [27]. Moreover, owning 58% of the world's palm oil production, Indonesia and Malaysia are considered the two largest producers [14]. In 2016, extracting 17.32 million tonnes of crude palm oil from the palm oil mills, oil palm plantations in Malaysia have surged to 5.74 million hectares [28]. POME is a potential source of biofuel production in Malaysia by approximately 58 million tons annually. Besides this, other agricultural products such as rubber, paddy, and different palm oil products have the potential to be used as sources of biofuel production. The oil palm residues are oil palm trunks, empty fruit bunches (EFBs), crude palm oil, oil palm trunk (OPT), Palm Kernel Shell (PKS), and mesocarp fiber [2]. Palm oil mill biomass mainly consists of cellulose (24-65%), hemicellulose (21-34%), and lignin (14-31%) [29]. The high cellulose content of oil palm biomass oil makes it a suitable source to produce different types of biofuels. Table 1 shows the oil palm plantation area based on the states in Malaysia.

Biofuel Production in Malaysia
In the following sections, the potential of each biofuel type (bioethanol, biodiesel, biohydrogen, and biogas) in Malaysia is summarized and discussed. Then, the impact of these fuels on the Greenhouse Gas (GHG) emissions is investigated, and so are the government strategies and policies based on current development programs that have been undertaken.

Bioethanol Production
One of the most beneficial biofuels is bioethanol, which can be substituted for fossil fuels [31,32]. Due to their low-cost production and lower GHG emissions, sugar-based feedstocks are a valuable candidate to be used for bioethanol production with a high yield [33,34]. Due to the existence of the rich tropical biodiversity in Malaysia and also the higher consumption of bioethanol by vehicles rather than biodiesel, the bioethanol market of Malaysia has gained attention from researchers [35,36].
In Malaysia, the technology for ethanol production has not been fully commercialized due to several barriers, such as (1) the high transportation cost from rural plantations to urban processing plants, (2) the high capital investment, and (3) the lack of advanced technology [37]. However, after the promulgation of biofuel policies in 2006 and the increase in palm oil production, a significant growth in bioethanol production was observed [2,38]. Biomass such as lignocellulosic material is used as a renewable energy source [39,40]. Lignocellulosic biomass contains lignin, cellulose, and hemicellulose in a complex structure. It requires taking a pretreatment step to break down the bonds inside the polymers to smaller subunits [39]. The bioethanol production process includes 1-pretreatment; 2-hydrolysis; 3-fermentation; 4-distillation and ethanol recovery. In the pretreatment step, the lignocellulosic compounds deform the cellulose, lignin, and hemicellulose structure and degrade the crystallinity degree to make it ready for the hydrolysis step [41,42]. During hydrolysis, enzymes are used to cleave the carbohydrate chains, which has a direct effect on the quality of ethanol production [43,44]. Other influencing parameters for second-generation bioethanol production are the capital cost of the plant and the cost of feedstock, enzyme, and energy [45].
In the next step, microorganisms use the available sugars in pretreated biomass in the fermentation step. This process can be considered as Separate Hydrolysis and Fermentation (SHF) and Simultaneous Saccharification and Fermentation (SSF). In the SHF process, the separation of the fermentation and hydrolysis process occurs in two different stages, while SSF happens simultaneously [46]. Figure 2 illustrates the bioethanol production pathway from lignocellulosic materials. pretreatment step, the lignocellulosic compounds deform the cellulose, lignin, and hemicellulose structure and degrade the crystallinity degree to make it ready for the hydrolysis step [41,42]. During hydrolysis, enzymes are used to cleave the carbohydrate chains, which has a direct effect on the quality of ethanol production [43,44]. Other influencing parameters for second-generation bioethanol production are the capital cost of the plant and the cost of feedstock, enzyme, and energy [45].
In the next step, microorganisms use the available sugars in pretreated biomass in the fermentation step. This process can be considered as Separate Hydrolysis and Fermentation (SHF) and Simultaneous Saccharification and Fermentation (SSF). In the SHF process, the separation of the fermentation and hydrolysis process occurs in two different stages, while SSF happena simultaneously [46]. Figure 2 illustrates the bioethanol production pathway from lignocellulosic materials. The influential factors on bioethanol production can be listed as temperature, pH, and fermentation time. The ideal temperature and optimal pH range in the fermentation process to produce ethanol should be between 20 and 35 ℃ and 4 and 5, respectively [47]. In 2015, Aditiya et al. [48] used mechanical and acid pretreatment for rice straw biomass at 90 ℃ for 60 min, which had total glucose of 11.466 g/L. Additionally, the combination of acid pretreatment with enzymatic hydrolysis yielded a higher ethanol content (52.75%) than the acidic pretreatment (11.26%). In another study, Jung et al. [49] used oil palm empty bunches under dilute sulfuric acid pretreatment and microwaving at 190 ℃, which led to a total glucose yield of 88.5% and an ethanol yield of 52.5%. Table 2 presents the recent studies of bioethanol production from different sources in Malaysia. Table 2. Recent studies on bioethanol production from different sources in Malaysia.  The influential factors on bioethanol production can be listed as temperature, pH, and fermentation time. The ideal temperature and optimal pH range in the fermentation process to produce ethanol should be between 20 and 35 • C and 4 and 5, respectively [47]. In 2015, Aditiya et al. [48] used mechanical and acid pretreatment for rice straw biomass at 90 • C for 60 min, which had total glucose of 11.466 g/L. Additionally, the combination of acid pretreatment with enzymatic hydrolysis yielded a higher ethanol content (52.75%) than the acidic pretreatment (11.26%). In another study, Jung et al. [49] used oil palm empty bunches under dilute sulfuric acid pretreatment and microwaving at 190 • C, which led to a total glucose yield of 88.5% and an ethanol yield of 52.5%. Table 2 presents the recent studies of bioethanol production from different sources in Malaysia.

Biodiesel Production
As a liquid biofuel and non-pollutant fuel, biodiesel could be regarded as an alternative to conventional fuels because of its advantages over fossil fuels [2]. One of the most significant benefits of biodiesel is its renewability property, which allows it to have lower toxicity levels and pollutant emissions. Being degradable biologically, and its applicability to be used as an engine fuel are other advantages of this fuel [58,59].
Due to the high production of palm oil in the world, Malaysia has been considered as one of the top palm-based biodiesel producers [60,61]. Producing biodiesel in this country dates back to late 2005 due to the lower price of crude palm oil compared to that of crude oil [61]. Malaysia has a promising trend only for biodiesel by 490 million liters in 2017, which is expected to reach 815 million liters in 2027, showing a 66% increase [62,63].
Indeed, based on the type and availability of the feedstock, biodiesel could be classified into two main groups, called edible and non-edible (vegetable oil, algal oil, waste animal oil, waste cooking oil) [64]. Different feedstocks could be used to produce biodiesel [63]. The first, second, and third generation of biodiesel are produced from edible oil, non-edible oil feedstocks, and algae oil, respectively. Compared to edible and non-edible feedstocks, algae has a high oil content (30-70%), which made it suitable for biodiesel production [63,65].
Besides this, selecting the feedstock to produce biodiesel mainly depends on the economic aspect of the country and the availability of the feedstock, which should be considered before deciding to select it. In Malaysia, the most common sources for biodiesel production are palm oil and coconut oil, while India uses non-edible vegetable oils, such as Jatropha, Simarouba, and Karanja [57,66,67].
The composition and purity of the obtained biodiesel are determined based on the used feedstock [63]. Besides this, the molar ratio (alcohol to oil), reaction temperature, time, concentration, and type of catalyst are the most influential factors in the production process [58]. There are two different approaches for biodiesel production: either single-(transesterification) or two-(ester-transesterification) step processes [68]. Due to its lower production cost, higher yield, and a more sustainable pathway, transesterification is the most common method for biodiesel production [58]. Producing biodiesel via transesterification includes the catalytic hydro processing of triglycerides and the thermal conversion of lignocellulose, employing gasification and pyrolysis [69]. The esters and glycerol are produced in transesterification from the reaction of triglyceride (free fatty acid) and alcohol. Based on the weight of biodiesel and glycerol, they settle on the top and bottom, after separating the end products of the transesterification [70]. The schematic process is shown in Figure 3 [58].  The recent studies on biodiesel production from different sources in Malaysia are reported in Table 3.  The recent studies on biodiesel production from different sources in Malaysia are reported in Table 3.

Biohydrogen Production
Hydrogen is an alternative renewable energy source that produces (122 kJ/g) energy and zero-carbon emission [79]. It can be replaced by non-renewable energy sources that will contribute about 8-10% of the world's total energy by 2025, as estimated by the US Department of Energy [80,81]. Hence, several studies have been conducted on biohydrogen production from lignocellulose biomass such as oil palm biomass. In a developing country such as Malaysia, biohydrogen could serve as a remarkable clean energy source. Therefore, hydrogen is getting more attention as a promising replacement for existing fossil fuels. Based on the estimation, Malaysia will have the highest mean real output growth rate of the biohydrogen sector from 2017 to 2040 of 5.02%, in comparison with countries such as Japan, China, India, and Korea [82].
The bio-conversion of lignocellulose biomass into hydrogen requires several pretreatment methods that include physical and chemical approaches for the delignification of the biomass [83]. For example, during the thermochemical process, temperature and pressure are maintained to change the structure of biomass chemically to be able to produce a high yield of hydrogen. Gasification and pyrolysis are amongst well-known thermochemical methods to produce hydrogen [84]. Hence, the biological processes encompass the presence of microorganisms during the fermentation process to break down the polysaccharides towards producing hydrogen [85,86].
The fermentative bacterium can convert complex organic wastes into hydrogen energy during the fermentation process. Therefore, the maximum yield of H 2 production has proportional influenced by the bacterial inoculum, substrate, and other physical parameters [87,88]. Facultative and obligate anaerobes are promising microorganisms for hydrogen production via fermentation. Particularly, facultatively anaerobic bacteria such as Enterobacter aerogenes and Bacillus sp effectively produce hydrogen in fermentation processes. Figure 4 illustrates the pathway conversion of palm oil mill waste to biofuel production, with an emphasis on producing hydrogen [89].
Oil palm mill wastes consist of cellulose and hemicellulose. They are rich in pentose and hexose, which makes them favorable substrates to produce biohydrogen. For instance, POME contains high concentrations of free fatty acids, which is a suitable substrate to be used in the fermentation process for hydrogen production [90]. Several studies reported the biohydrogen production from POME. As reported by Abdullah et al. [91], a 22% increase in hydrogen production with a yield of 1.88 mol H 2 /mol sugar obtained as POME was used as the feedstock. In addition, adjusting the C/N ratio can help to boost hydrogen production. It should be noted that excessive nitrogen could inhibit hydrogen production, while lower concentrations also cause nutrient deficiency and low-yield change the structure of biomass chemically to be able to produce a high yield of hydrogen. Gasification and pyrolysis are amongst well-known thermochemical methods to produce hydrogen [84]. Hence, the biological processes encompass the presence of microorganisms during the fermentation process to break down the polysaccharides towards producing hydrogen [85,86].
The fermentative bacterium can convert complex organic wastes into hydrogen energy during the fermentation process. Therefore, the maximum yield of H2 production has proportional influenced by the bacterial inoculum, substrate, and other physical parameters [87,88]. Facultative and obligate anaerobes are promising microorganisms for hydrogen production via fermentation. Particularly, facultatively anaerobic bacteria such as Enterobacter aerogenes and Bacillus sp effectively produce hydrogen in fermentation processes. Figure 4 illustrates the pathway conversion of palm oil mill waste to biofuel production, with an emphasis on producing hydrogen [89]. Oil palm mill wastes consist of cellulose and hemicellulose. They are rich in pentose and hexose, which makes them favorable substrates to produce biohydrogen. For instance, POME contains high concentrations of free fatty acids, which is a suitable substrate to be used in the fermentation process for hydrogen production [90]. Several studies reported the biohydrogen production from POME. As reported by Abdullah et al. [91], a 22% increase in hydrogen production with a yield of 1.88 mol H₂/mol sugar obtained as POME was used as the feedstock. In addition, adjusting the C/N ratio can help to boost hydrogen production. It should be noted that excessive nitrogen could inhibit hydrogen production, while lower concentrations also cause nutrient deficiency and low-yield hydrogen production. Additionally, the C/N ratio directly affects the growth of microbes during the fermentation process for effective biohydrogen production [92].
Mishra et al. [93] isolated a novel mesophilic bacterial strain from palm oil mill sludge obtained from the FELDA palm oil industry in Pahang, Malaysia. The strain can produce the highest hydrogen yield of 2.42 mol H2/mol glucose. From results, indigenous isolated strains from palm oil biomass showed more positive impacts on hydrogen production compared to the exogenous sources. It is worth noticing that using a genetically modified organism like Escherichia coli (E. coli) is another useful pathway for increasing the substrate fermentation rate in hydrogen production [94]. Table 4 shows recent studies of biohydrogen production from POME in Malaysia.  Mishra et al. [93] isolated a novel mesophilic bacterial strain from palm oil mill sludge obtained from the FELDA palm oil industry in Pahang, Malaysia. The strain can produce the highest hydrogen yield of 2.42 mol H 2 /mol glucose. From results, indigenous isolated strains from palm oil biomass showed more positive impacts on hydrogen production compared to the exogenous sources. It is worth noticing that using a genetically modified organism like Escherichia coli (E. coli) is another useful pathway for increasing the substrate fermentation rate in hydrogen production [94]. Table 4 shows recent studies of biohydrogen production from POME in Malaysia.

Biogas Production
Biogas is one of the most promising bioenergy alternatives to replace fossil fuels. It is a mixture of two principal gases, called methane and carbon dioxide. When the share of methane in biogas is more than 40%, it would be highly flammable and could be considered as a renewable energy source [100,101]. Many renewable sources such as lignocellulosic materials that include agricultural residues can be used to produce biogas due to their potential and characteristics [102]. There are several resources for the production of biogas, including industrial wastewater, urban wastewater, municipal solid waste, and lignocellulosic wastes [103]. Several biomass resources are available in Malaysian industries, such as palm oil, paddy, sugar, and wood. Palm oil industries produce 59.8 million tons of biomass per year, which is the most significant biomass source in Malaysia (Table 5). Biogas can be produced in an anaerobic digestion process. Organic compounds are decomposed into simpler compounds by anaerobic microorganisms. Then, in several consequent biological processes, these compounds are converted to final products such as methane, carbon dioxide, hydrogen, nitrogen, and hydrogen sulfide [106][107][108]. Anaerobic digestion is used to produce biogas from a wide range of materials, such as organic solid waste, agricultural biomass, food, and animal feces [104,109]. The biological conversion of biogas from organic wastes to generate bioenergy has several advantages over other forms of energy production from biomass. Those methods could be listed as biological biodiesel production from biomass, the incineration of biomass or biohydrogen, biobutanol, and bioethanol production [110]. Biologically, biogas is produced by a consortium of microorganisms in four stages, called hydrolysis, acidogenesis, acetogenesis, and methanogenesis ( Figure 5). processes, these compounds are converted to final products such as methane, carbon dioxide, hydrogen, nitrogen, and hydrogen sulfide [106][107][108]. Anaerobic digestion is used to produce biogas from a wide range of materials, such as organic solid waste, agricultural biomass, food, and animal feces [104,109]. The biological conversion of biogas from organic wastes to generate bioenergy has several advantages over other forms of energy production from biomass. Those methods could be listed as biological biodiesel production from biomass, the incineration of biomass or biohydrogen, biobutanol, and bioethanol production [110]. Biologically, biogas is produced by a consortium of microorganisms in four stages, called hydrolysis, acidogenesis, acetogenesis, and methanogenesis ( Figure 5). The stage of organic waste hydrolysis can be carried out using several strains of bacteria such as Microbispora, Acetovibrio, Bacteriodes, Ruminococcus, Thermomonospora, Bacillus, Cellulomonas, and Clostridium [111]. Methanogenesis microorganisms are sensitive to pH. The optimal pH for methanogenesis microorganisms is from 7.2 to 7.8. In a pH below 6.8, the methane production is stopped by methanogens. The highest biogas production rate is usually between 41 and 43 °C, but can be increased for some substrates in thermophilic fermentation (52 °C to 56 °C) [101]. Table 6 shows recent studies of biogas production from different sources in Malaysia.  The stage of organic waste hydrolysis can be carried out using several strains of bacteria such as Microbispora, Acetovibrio, Bacteriodes, Ruminococcus, Thermomonospora, Bacillus, Cellulomonas, and Clostridium [111]. Methanogenesis microorganisms are sensitive to pH. The optimal pH for Processes 2020, 8, 993 9 of 19 methanogenesis microorganisms is from 7.2 to 7.8. In a pH below 6.8, the methane production is stopped by methanogens. The highest biogas production rate is usually between 41 and 43 • C, but can be increased for some substrates in thermophilic fermentation (52 • C to 56 • C) [101]. Table 6 shows recent studies of biogas production from different sources in Malaysia.

Life Cycle Assessment and GHG Emission
Controlling environmental issues (especially GHG emissions) and recovering energy from organic wastes has drawn more attention. Therefore, to reduce GHG emissions, governments and policymakers have been initiated several policies to switch from conventional (fossil) fuels to renewable energy sources [120]. Malaysia also followed this trend by formulating the National Biofuel Policy (NBP) to reduce the country's reliance on fossil fuels. Based on this policy, the 5% palm-based biodiesel blend in conventional fossil diesel is mandatory, which helps the transport sector to reduce 1.03 million tonnes of GHG (4.9% of total road-emissions) [121,122]. Moreover, to commit to the obligations of the Paris Agreement, which was signed in 2016, the government should reduce its GHG emissions by 45% by 2030 compared to the Business as Usual (BaU) scenario in 2005 [123]. It should be noted that about 60% of GHG emissions are caused during the crop plantation stages, such as irrigation, fertilizer usage, and the diesel used for vehicles [124].
To achieve this goal, Malaysia has underlined a strategy to support the oil palm industry. As mentioned before, this industry is one of the most significant industrial sectors and is the growth engine of the country. Moreover, this strategy can relieve the environmental side effects [125]. Meanwhile, as reported by Abdul-Manan [125], the GHG saving from palm-based fuel has a limited effect due to the high demand for energy in Malaysia.
The Life Cycle Assessment (LCA) is a systematic tool to evaluate and analyze the environmental impacts of a product and the whole production process [126,127]. In the next sections, the LCA analysis for different biofuel production in Malaysia is discussed.

LCA Analyses of Bioethanol Production
Several studies have been evaluated the GHG emission rate for bioethanol production from different sources in Malaysia using LCA analysis. For instance, the potential of bioethanol production from a lignocellulosic source (Sri Kanji 1 cassava) for energy efficiency, and the GHG emissions reduction was evaluated. Based on the results, the bioethanol production from Sri Kanji 1 cassava was energy-efficient, due to having a 25.68 MJ/L energy balance and a 3.98 MJ/L net energy ratio. They found that 73.2% of GHGs can be reduced by the conversion of one liter of cassava to ethanol [128]. In another study, the environmental aspects of bioethanol production from oil palm were evaluated using the LCA SimaPro 8 software [129]. They found that fermentation and transportation were the main contributors to fossil fuel energy consumption, in the range of 52-97%.

LCA Analyses of Biodiesel Production
Evaluating the environmental impacts of palm and Jatropha biodiesel production in Malaysia dates back a long time. As reported by Lam et al. [124], considering the whole life cycle chain, the CO 2 sequestration from palm biodiesel was 20 times higher than that from jatropha biodiesel. In addition, 1 tonne of palm biodiesel production generated 43% more energy than Jatropha biodiesel. Another study has evaluated the energy ratio and CO 2 emissions of palm and rapeseed biodiesel by employing the LCA approach. Based on the results, the energy ratio for palm biodiesel and rapeseed was obtained at 3.53 and 1.44, respectively, showing the suitability of palm biomass to produce biodiesel. Besides, the combustion of palm biodiesel as an eco-friendly fuel should cause a 38% reduction in CO 2 emission per liter of combusted fuel in comparison to petrol [130]. Recently, Farid et al. [131] investigated the biodiesel production from WCO (Waste Cooking Oil) using a semi-industrial plant in Malaysia. Their results showed that if the price of B10 fuel was considered as USD 0.47/kg, the biodiesel production rate would be 3.68 million tonnes, at the level of 15 tonnes per batch per day with 330 production cycles annually.

LCA Analyses of Biogas Production
The conversion of POME into biogas can provide effective waste management and reduce the dependence on fossil fuel sources, simultaneously [132]. Based on the results of the LCA analysis and the environmental performance of biogas production from POME, achieving the goal of sustainable development in biodiesel production in a short time forced decision-makers to pay more attention to agricultural fields at the plantation stage [133]. Using the SimaPro 8.5 software and the ReCiPe 2016 method to conduct the LCA approach, a recent study has evaluated the biogas production from POME. The results revealed the need for improvements in upstream stages, focus on land use, and fertilizer production. Their results showed that 0.04 ha of land was required to produce one ton of FFB, which has an impact of 511.79 kg CO 2 -eq [133]. Sharvini et al. [134] evaluated the LCA of POME in two different treatment technologies, called the Continuous Stirred Tank Reactor (CSTR), and Covered Lagoon Bio-digester (CLB). The results supported the effectiveness of the CSTR system to capture CO 2 and SO 2 compared to the CLB system (Table 7). In another study, the LCA analysis of Malaysian oil palm products showed the production of one ton of FFB produced 119 kg CO 2 eq by using a yield of 20.7 tonnes oil FFB/ha. The production of one ton of crude palm oil with biogas capture emitted 506-971 kg CO 2 eq [135].

Government Strategies and Policies
Malaysia has accelerated its economic growth amongst the South East Asia countries. Indeed, it experienced a 5.4% growth per annum from 2010 to 2018. Moreover, gross national income per capita in Malaysia has reached more than USD $15,000 [136]. For the sake of the implementation of the development plans, this country has experienced high economic growth. More precisely, from 1970 to 2019 the Gross Domestic Product (GDP) has increased by 5.2 times (from 71.1 to 369.878 billion ringgits. However, the government and policymakers have paid more attention to economic activities and structured some strategies to boost the economy. Based on the estimation, the GDP would be increased from RM1.4 trillion in 2020 to RM2.6 trillion in 2030. Accordingly, the GDP per capita would rise more than two times (from RM 54.980 to RM117.260).
From the economic structure point of view, this country has been shifted from an agriculture-based economy to a manufacturing and services-based economy. Accordingly, the share of agriculture sector in GDP has been decreased from 31.8% to about 10%. Moreover, the economy will shift to knowledge-based and high value-added activities. Consequently, the share of skilled workers in total employment will be reached more than 40% in 2030 [137]. Increasing concerns about environmental issues, especially global warming, and pressures to achieve sustainable development goals have encouraged the government and policy-makers to follow the green-growth strategy [137].
Amongst the renewable sources in Malaysia, the biofuel sector is one of the most reliable engine drivers of economic growth. It is noteworthy to mention that despite approving the Five Fuel Diversification Policy in 2001, the share of renewable electricity was low (2%). Consequently, the government designed and structured the eleventh plan (2016-2020) based on the green growth strategy by expanding renewable sources. In this respect, the government has set a new goal to increase this share to 20% by 2025. The government has provided the RM 1.09 ($0.28/L) subsidy to produce biodiesel in order to meet the target of controlling GHG emissions and its Paris agreement obligations [138]. In addition, 10 million tonnes of production of palm-based biodiesel in a year has been targeted by the Malaysian authorities [139].
The laboratory-scale experiments conducted in Malaysia showed the significant production of hydrogen from oil palm mill wastes. Hence, it can provide a platform for the large-scale production of biohydrogen at the industrial level as a sustainable and environmentally friendly energy. Achieving this governmental policy is vital to support the plantation towards developing biofuel technologies [83]. Besides this, to decrease GHG emissions in the agriculture sector, the government implemented two new regulations, called the management of used POME in the palm oil mill industries and preventing deforestation [28]. To control the environmental issues caused by road traffic emissions, the usage of energy-efficient vehicles, and biofuels, the government set the EURO 4M standards in 2013, and implemented them in RON97 in 2015. Hence, according to the eleventh plan, the EURO 5 standards and B15 (15% biodiesel blending) should be performed in 2020 [140].

Conclusions and Future Perspectives
This paper reviewed the status of biofuel production in Malaysia as one of the top biofuel producers due to having abundant natural sources along with rapid economic growth in South East Asia. The following conclusions can be drawn:

•
Lignocellulosic biomass and POME are two main sources to produce different biofuel types.

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Their potential is comparable with other sources in other countries and could be considered as green since they can reduce GHG significantly.

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Bioethanol production in Malaysia is based on using different woody and lignocellulosic biomass with the range of 0.3-4.5 g/g biomass to ethanol. • An LCA analysis revealed the effectiveness of palm-based biodiesel compared to petrol in terms of energy output and GHG reduction.

•
As biofuel production and export are increasing year by year in Malaysia, the government needs to have some initiatives for stakeholders to facilitate their production by providing advanced technologies.

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The outlook of biofuel in Malaysia depends on several sectors, such as the government, industries, and stakeholders, which need more integration to reach the country's needs.

Conflicts of Interest:
The authors declare no conflict of interest.