E ﬀ ects of Di ﬀ erent Drying Methods and Temperature on the Drying Behavior and Quality Attributes of Cherry Laurel Fruit

: This study aimed to investigate the e ﬀ ect of di ﬀ erent drying methods and drying temperature on the drying kinetics, total bioactive compounds, phenolic proﬁle, microstructural properties, rehydration kinetics, and color change of cherry laurel fruit. For this aim, hot air drying (HAD), ultrasound-assisted vacuum drying (USV), and freeze-drying (FD) were conducted on drying of cherry laurel. HAD and USV were conducted at 50, 60, and 70 ◦ C. Drying times of the samples were 1980, 1220, and 770 min for HAD at 50, 60 and 70 ◦ C, and 950, 615, and 445 min at 50, 60, and 70 ◦ C, respectively, for USV. The total bioactive compound was signiﬁcantly a ﬀ ected by both drying methods and temperature ( p < 0.05). FD exhibited the highest total phenolic (TPC), total ﬂavonoid (TFC), total anthocyanin (TAC), and antioxidant capacity value USV showed a higher amount of bioactive compounds than those of HAD at the same drying temperature. The content of total bioactive compounds signiﬁcantly increased as the temperature increased for both HAD and USV ( p < 0.05). The chlorogenic acid was identiﬁed as a major phenolic, and its amount signiﬁcantly depended on drying methods ( p < 0.05). SEM images described the surface characteristic of dried samples. HAD dried products showed higher shrinkage compared to FD and USV. All drying methods signiﬁcantly a ﬀ ected the total color di ﬀ erence ( ∆ E) values ( p < 0.05). This study proposed that USV could be as an alternative method to HAD due to higher bioactive compounds retention and rehydration ratio, shorter drying time, less color change, and shrinkage formation.


Introduction
Cherry laurel (Prunus laurocerasus L.) belonging to the Rosaceae family is a well-known fruit grown in various countries of the world. It can be consumed as fresh or dried and pickled form and processed into various products such as pekmez, jam, marmalade, and fruit juice [1]. Cherry laurel is a bioactive compounds-rich fruit including phenolics, anthocyanins, and vitamins. Cherry laurel is abundant in procyanidins which have a decisive role in preventing cardiovascular disease [1]. Procyanidin content of cherry laurel was found higher than that of the other cherry fruits [2]. For this reason, cherry laurel fruits have attracted attention in recent years. Fresh cherry laurel is perishable, and the shelf life of fruits is low due to its high water content. Therefore, a preservation method should be applied to increase the shelf life and supply consumption throughout the year.
Drying is one of the widely used preservation methods to increase the shelf life of many fruits and vegetables. The drying process has several advantages such as lowering storage volume, and extending shelf life and ensuring the microbial safety of biological products [3]. Besides, commercial interest on dried fruits, mainly containing bioactive compounds, has been increased. The drying techniques

Materials
Fresh cherry laurel fruits (Prunus laurocerasus L.) were supplied from the Ministry of Agriculture and Forestry Black Sea Agricultural Research Institute (Samsun, Turkey) when fully ripe in August 2019. The genotype of 61K06 was obtained because of its high phenolic content (Halilova and Ercisli, 2010). Cherry laurel fruits with uniform shape, color, and size, free from visible damage and blot were selected for analyses. The fruits were detached, washed with water, and blotted gently. The average length, width, and height of fruit were 1.30 cm, 1.15 cm, 1.14 cm, respectively. The average core weight was determined as 0.53 g. The fruits were stored at +4 • C before the drying process. The dried fruits were stored in a desiccator after cooling at a temperature of 25 • C until the extraction process and other analyses.

Determination of Dry Matter Content and Drying Time
Cherry laurels samples were dehydrated with three different methods namely, freeze-drying (FD) and hot-air drying (HAD) and ultrasound-assisted vacuum drying (USV). Cherry laurels samples dried as a whole. The drying process for HAD and USV was carried out at 50, 60, and 70 • C temperatures. The air velocity was constant at 1.3 m/s for HAD and measured by a Testo 440 probe anemometer (Lutron, AM-4201, Taiwan). FD was performed according to the standard program of freeze-dryer (Martin Christ, Beta 1-8 LSCplus, Osterode am Harz, Germany) at −55 • C and 1 hPa for 72 days. USV was performed according to the method of TekinandBaslar [11]. In the USV system, an ultrasonic water bath (Daihan, WUC-D10H, Gangwondo, South Korea) was used to control drying temperature, and a vacuum pump (EVP 2XZ-2C, Zhejiang, China) was used to reduce pressure. The properties of ultrasonic water bath (10 L) were with an amplitude of 100%, and power intensity of 1 W/cm 2 , and a vacuum pump provides 15 mbar pressure. The samples were placed into the conical flask connected to the vacuum pump and sonication was applied at 40 kHz by the ultrasonic water bath. A thermocouple (k-type, Omega Engineering Inc., Norwalk, Connecticut, USA) was used to measure the temperature of the water bath adjusted by the manual circulation of the bathwater. The drying time was 950, 615, and 445 min at 50, 60, and 70 • C, for USV. Water loss of the cherry laurel samples was measured at 30 min intervals for HAD and USV. The samples were weighted by a digital balance (Mettler-Toledo AG, Grefensee, Switzerland, model BB3000 with ±0.1 g). The drying process was completed when the final moisture content of the samples achieved to~0.2 kg water/kg dry matter (DM.). The experiments were performed in triplicate, and the average moisture content values were used to plot the drying curves for HAD and USV. To determine dry matter content, cherry laurels were dehydrated in the oven at 105 • C until it reached a constant weight. The dry matter content of cherry laurels was determined by weighing the constant mass (19.64 %DM).

Extraction Procedure
The methanol-water (50:50) solution was used for the extraction solvent of fresh and dried samples, and solid:solvent ratio was 1:10 (w/v). The mixture was homogenized by an ULTRA-TURRAX (Daihan, HG-15D, Gangwondo, Korea) at 10,000 rpm for 3 min, followed by 2 h of shaking at a temperature of 25 • C. At the end of the extraction, the mixture was centrifuged at 3920× g (Hettich 320R, Tuttlingen, Germany) and the supernatant was filtered by a 0.45 µm filter. The final extracts were stored at −20 • C until further analysis.

Determination of Total Phenolic Content (TPC)
For the determination of TPC value, the modified method reported by Singleton [15] was followed. Folin-Ciocalteu's phenol reagent solution (0.2 N) was prepared. Sample extracts were diluted with distilled water as a ratio of 1:60 (v/v). 2.5 mL reagent solution and 2.0 mL Na 2 CO 3 (7.5%) were added into a tube containing 0.5 mL the diluted extracts. The tubes were stored for 60 min in dark at room temperature (25 • C). The absorbance values of the samples were recorded at 760 nm using a UV/VIS Spectrophotometer (UV-1800; Shimadzu, Japan). The result of the TPC was presented as mg gallic acid equivalents (GAE) per 100 g samples (DM).

Determination of Total Flavonoid Content (TFC)
For the determination of TFC, the method described by Zhishen, et al. [16] was followed. 1 mL of diluted extract (1:30, v/v) was mixed with 4 mL distilled water. Afterwards, 0.3 mL NaNO 2 (5%), 0.3 mL AlCI 3 (10%) and 2 mL 1 M NaOH were added to the mixture. The total volume was completed to 10 mL with distilled water and mixed with a vortex for about 1 min. The absorbance value of the samples was recorded at 510 nm by the UV-VIS Spectrophotometer, and the results were expressed as mg catechin/100 g samples (DM).

Total Anthocyanin Content (TAC)
TAC value was determined by the pH-differential method explained by GiustiandWrolstad [17]. and 3.2 mL of buffer solutions (pH 1.0 and pH 4.5) was mixed with 0.8 mL diluted extracts (1:4, v/v). The mixture was stored in dark for 30 min at room temperature. The absorbance values of the samples were measured at 510 and 700 nm using the UV-VIS Spectrophotometer A molar extinction coefficient and molecular weight of cyanidin-3-glucoside (29,600 and 445.2 g/mol, respectively) were used of for the determination of TAC. The results were given as mg cyanidin-3-glucoside per 100 g samples (DM).

Antioxidant Capacity by DPPH Method
Antioxidant capacities (AA) of the samples were analyzed by the DPPH method described by Singh, et al. [18]. 0.1 mM ethanolic DPPH solution was prepared, and 0.1 mL of each diluted extract was mixed with 4.9 mL DPPH solution, and samples stored at a temperature of 25 • C for 30 min in dark conditions The values of absorbance was measured at 517 nm using the UV-VIS Spectrophotometer (The result of AA was given as mg Trolox equivalent (TE) per 100 g samples (DM)

Determination of Phenolic Profile
The individual phenolic compounds of the cherry laurel sample were determined by the HPLC system coupled to a diode array (HPLC-DAD, Shimadzu Corp., Kyoto, Japan). HPLC system consisted of an LC-20AD pump, an SPDM20A DAD detector, a SIL-20A HT autosampler, a CTO-10ASVP column oven, a DGU-20A5R degasser, and a CMB-20A communications bus module (Shimadzu Corp., Kyoto, Japan). A reversed-phase column (Intersil ® ODS C-18, GL Sciences, Tokyo, Japan) with a 250 mm × 4.6 mm length, 5 µm particle size was used to perform separation of phenolic compounds at 40 • C. The mobile phases consisted of solvent A (distilled water with 0.1% (v/v) acetic acid) and solvent B (acetonitrile with 0.1% (v/v) acetic acid). Gradient elution was applied at a flow rate of 1 mL/min and it was: 10% B (0 to 2 min), 10% to 30% B (2 to 27 min), 30% to 90% B (27 to 50 min) and 90% to 100% B (51 to 60 min). Chromatograms were obtained at 254-356 nm. Identification and quantification analysis were conducted based on standard curves and retention times and. The detection of phenolic compounds was performed at 260 nm (epicatechin), 280 nm (gallic, syringic, protocatechuic, caffeic, cinnamic, p-hydroxybenzoic acid and catechin), 320 nm (chlorogenic, vanillic, p-coumaric, ferulic acid) and 360 nm (rutin, quercetin). The concentration of each phenolic compound was expressed as mg per 100 g of sample (DM) [12].

SEM Analysis
The surface structures of samples were evaluated with Scanning Electron Microscopy (SEM). The microstructure of the dried samples was characterized by a field emission scanning electron microscope (FESEM, FEI QUANTA FEG 250). Dried cherry laurel samples were cut and fixed on the SEM stub and covered with a golden coat to supply a reflective surface for the electron beam [19].

Color Measurement
The color values of fresh and dried samples were measured using a colorimeter (CR-400 Konica, Minolta, Tokyo, Japan). L*, a* ve b* parameters of the samples were measured after calibrating at standard illuminant (D65). The total color difference index (∆E) calculated by the following equation was used to define the change in the color values:

Determination of Rehydration Ratio
To determine the rehydration ratio (RR), 2 g of the dried samples was immersed in 20 mL distilled water at 25 • C for 360 min and drained (Deng, 2017). The RR value was calculated by the equation: here, M is the sample weight after rehydration; M 0 is the sample weight before rehydration. The rehydration kinetic of the dried samples was modeled by Peleg model: where M is moisture content at time t in (% DM.); M 0 is the initial moisture content in (% DM); k 1 is the Peleg rate constant in (min % DM −1 ); k 2 is the Peleg capacity constant in (% DM −1 ). Peleg model parameters were calculated by nonlinear regression analysis using the Statistica software program (StatSoft, Inc., Tulsa, OK, USA).

Statistical Analysis
The statistical analysis was carried out using the Statistica software program (StatSoft, Inc., Tulsa, OK, USA). All the analyses were conducted in triplicate. The standard error and mean value were calculated. ANOVA was used to determine the statistical difference between samples. Duncan, multiple comparison tests at a 95% significance level was performed to evaluate the effect of different drying temperatures and methods on the evaluated properties. Figure 1 shows the drying kinetics of the samples at different temperatures with HAD and USV. Drying time of the samples was 1980, 1220, and 770 min for HAD at 50, 60, and 70 • C and 950, 615, and 445 min at 50, 60, and 70 • C, respectively, for USV. In both drying methods, upon the Increase in temperature, the moisture ratio (kg water/kg DM) was increased, and consequently, the drying time was decreased. Along with the rise in temperature, the decrease in drying time has also been reported in previous studies [20,21]. When we compare HAD and USV at the same temperature, much higher moisture loss and shorter drying time were observed by USV. In both drying methods, a constant drying behavior was observed initially, while a falling rate period was observed towards the end of the drying time (Figure 1). At 60 and 70 • C, the falling rate period was shorter for USV than HAD Besides, at the highest temperature applied (70 • C) HAD exhibited similar drying kinetics to USV at 50 • C, at the lowest temperature applied (Figure 1), indicating that USV showed a much faster drying rate compare to HAD. This result can be explained by the fact that the cavitation effect associated with the ultrasound process disrupts the food matrix and creates pores therefore the water can easily move away [11]. For USV, the vacuum process could have also affected the drying behavior of samples by decreasing the boiling point and increasing the evaporation rate. The decrease in the drying time of the USV process compared to HAD has also been reported in previous studies [11,12,22].  Table 1 shows the effect of different drying methods (HAD, USV, and FD) and drying temperatures on the TPC, TFC, TAC, and DPPH value of the samples. The drying temperature and drying method (HAD and USV) significantly affected the amount of total bioactive compounds (p < 0.05). The TPC, TFC, and TAC content of fresh samples were 839.66 (mg GAE/100 g DM), 1868.963 (mg catechin/100 g DM) and 130.41 (cyanidin 3-glucoside/100 g DM) respectively. As seen in Table 1, except for FD, a significantly important reduction in the amount of all bioactive compounds was observed in all drying conditions (p < 0.05). FD exhibited the highest retention in bioactive compounds. This result was the agreement with other studies [3,12,23]. The higher bioactive compounds in FD can be explained by the low temperatures and under vacuum conditions. In these conditions, the samples are dried without being exposed to thermal degradation and oxidation. The decrease in bioactive compounds in HAD and USV was significantly affected by the temperature (p < 0.05). Compared to the fresh samples, the decrease in the level of bioactive components in dried fruits can be explained by oxidation, thermal degradation, and an increase in polyphenol oxidase activity [24]. For the same drying method either by USV or HAD, the level of bioactive components increased as the temperature elevated. It can be explained that higher temperatures reduced the drying time e.g., in HAD, it was 1980 min while the temperature was 50 ℃, and 770 min while it was 70 ℃, meaning that the samples were subjected to less heat treatment during the whole drying process. Moreover, the rise in the temperature could damage the food structure, and bioactive compounds bound to the matrix could be better released into the extraction medium [25]. It was also known that higher temperatures could denature the polyphenol oxidase enzyme that is responsible for the degradation of phenolic compounds, therefore would increase their retention [26].

Effect of Drying Methods on Bioactive Properties of Cherry Laurel
At the same temperature, USV showed a higher amount of bioactive compounds than those of HAD, and this difference was significant (p < 0.05). It can be explained that the drying times observed in USV are shorter than the HAD (e.g., at 50 ℃, the drying time for USV was 445 min. and for HAD it was 770 min) and the samples are exposed to less thermal process [12]. Besides, the cavitation effect and formation of microchannels may have led to the release of more phenolics bound to the matrix and an improvement in the extraction of phenolic compounds. The higher retention of phenolic compounds by ultrasound-assisted drying was also reported in the previously published studies [10-  Table 1 shows the effect of different drying methods (HAD, USV, and FD) and drying temperatures on the TPC, TFC, TAC, and DPPH value of the samples. The drying temperature and drying method (HAD and USV) significantly affected the amount of total bioactive compounds (p < 0.05). The TPC, TFC, and TAC content of fresh samples were 839.66 (mg GAE/100 g DM), 1868.963 (mg catechin/100 g DM) and 130.41 (cyanidin 3-glucoside/100 g DM) respectively. As seen in Table 1, except for FD, a significantly important reduction in the amount of all bioactive compounds was observed in all drying conditions (p < 0.05). FD exhibited the highest retention in bioactive compounds. This result was the agreement with other studies [3,12,23]. The higher bioactive compounds in FD can be explained by the low temperatures and under vacuum conditions. In these conditions, the samples are dried without being exposed to thermal degradation and oxidation. The decrease in bioactive compounds in HAD and USV was significantly affected by the temperature (p < 0.05). Compared to the fresh samples, the decrease in the level of bioactive components in dried fruits can be explained by oxidation, thermal degradation, and an increase in polyphenol oxidase activity [24]. For the same drying method either by USV or HAD, the level of bioactive components increased as the temperature elevated. It can be explained that higher temperatures reduced the drying time e.g., in HAD, it was 1980 min while the temperature was 50 • C, and 770 min while it was 70 • C, meaning that the samples were subjected to less heat treatment during the whole drying process. Moreover, the rise in the temperature could damage the food structure, and bioactive compounds bound to the matrix could be better released into the extraction medium [25]. It was also known that higher temperatures could denature the polyphenol oxidase enzyme that is responsible for the degradation of phenolic compounds, therefore would increase their retention [26]. At the same temperature, USV showed a higher amount of bioactive compounds than those of HAD, and this difference was significant (p < 0.05). It can be explained that the drying times observed in USV are shorter than the HAD (e.g., at 50 • C, the drying time for USV was 445 min. and for HAD it was 770 min) and the samples are exposed to less thermal process [12]. Besides, the cavitation effect and formation of microchannels may have led to the release of more phenolics bound to the matrix and an improvement in the extraction of phenolic compounds. The higher retention of phenolic compounds by ultrasound-assisted drying was also reported in the previously published studies [10][11][12]27]. The DPPH radical scavenging ability of the samples depending on the drying method showed a similar trend with the total amount of bioactive compounds. Compared to fresh and FD samples, HAD and USV drying led to a reduction in antioxidant capacity. FD showed the highest antioxidant capacity while HAD showed the lowest value. For the same drying method, the rise in temperature also significantly increased the antioxidant capacity (p < 0.05) as a result of higher retention of phenolic compounds.

Effect of Drying Methods on Bioactive Properties of Cherry Laurel
In this study, the effect of the drying method on the phenolic profile was also investigated. Chlorogenic acid was the major phenolic compounds determined in cherry laurel, followed by epicatechin, vanillic acid, and syringic acid ( Table 2). These findings were similar to previous studies [1]. Compared to fresh samples, there was a significant decrease in individual phenolics in fruits dried by both HAD and USV methods. For HAD, at 50 • C, 60 • C and 70 • C, the chlorogenic acid content was reduced by 88, 82, and 79%. A similar trend was observed for USV dried samples at 50 and 60 • C, whereas at 70 • C the reduction was 14%. The reason for the higher concentration of chlorogenic acid in the extracts could be both related to the higher liberation of phenolics from the disrupted fruit matrix due to the ultrasound cavitation effect, and shorter drying time. In terms of epicatechin content, the second most abundant phenolics detected, the less reduction by the higher drying temperature was also observed in both HAD and USV methods. Compared to fresh samples, the higher level of phenolics was determined in FD fruits, which can be explained by ice crystal formation which could rupture the cell structure and led to the higher extraction rate [3].

The Effect on Microstructural Properties of Cherry Laurel
The SEM images showed the effect of different drying methods and temperature on the surface characteristics of dried fruits (Figure 2). Shrinkage is one of the primary physical defects that occur as a result of deterioration of the capillary structure during drying of food, and undesirable effects may occur in the texture, rehydration ability, and surface properties of the food [28].

The Effect on Microstructural Properties of Cherry Laurel
The SEM images showed the effect of different drying methods and temperature on the surface characteristics of dried fruits (Figure 2). Shrinkage is one of the primary physical defects that occur as a result of deterioration of the capillary structure during drying of food, and undesirable effects may occur in the texture, rehydration ability, and surface properties of the food [28]. Both the drying temperature and drying methods affected the shrinkage level of the samples. As can be seen from the figure, the highest shrinkage was observed in fruits dried by HAD compared to other methods. The higher shrinkage values during HAD was also reported from the previously published study [29]. The higher shrinkage could be due to a higher moisture gradient that occurred in HAD drying. The higher moisture gradient caused microstructure stress, collapsing of capillary, and irreversible structural change [30]. For HAD, application of drying temperature at 50 ℃ caused more shrinkage than those of 60 ℃ and 70 ℃ The longer drying time at 50 ℃ might have disrupted the cell wall and increased shrinkage level. HAD at 60 ℃ resulted in a lower shrinkage level than that of 70 ℃ . The optimum conditions related to both time and temperature might have resulted in lower shrinkage at 60 ℃. The increase in temperature from 60 ℃ to 70 ℃ might have caused collapsing of the capillary structure and forming of shrinkage.
For USV, the drying temperature of 70 ℃ caused a lower shrinkage than that of 50 ℃ and 60 ℃. For USV, drying temperature was a significant factor for the formation of shrinkage. For this reason, 50 ℃ and 60 ℃ drying temperatures should be selected to reduce shrinkage levels during USV drying. Both the drying temperature and drying methods affected the shrinkage level of the samples. As can be seen from the figure, the highest shrinkage was observed in fruits dried by HAD compared to other methods. The higher shrinkage values during HAD was also reported from the previously published study [29]. The higher shrinkage could be due to a higher moisture gradient that occurred in HAD drying. The higher moisture gradient caused microstructure stress, collapsing of capillary, and irreversible structural change [30]. For HAD, application of drying temperature at 50 • C caused more shrinkage than those of 60 • C and 70 • C The longer drying time at 50 • C might have disrupted the cell wall and increased shrinkage level. HAD at 60 • C resulted in a lower shrinkage level than that of 70 • C. The optimum conditions related to both time and temperature might have resulted in lower shrinkage at 60 • C. The increase in temperature from 60 • C to 70 • C might have caused collapsing of the capillary structure and forming of shrinkage.
For USV, the drying temperature of 70 • C caused a lower shrinkage than that of 50 • C and 60 • C. For USV, drying temperature was a significant factor for the formation of shrinkage. For this reason, 50 • C and 60 • C drying temperatures should be selected to reduce shrinkage levels during USV drying. The lower shrinkage level for USV than HAD could be due to the shorter drying time and less damage to the surface structure [31]. FD showed the lowest shrinkage compared to other drying conditions as reported in the previous studies [19,32,33]. USV 50 • C and 60 • C showed a similar surface characteristic to FD. The low shrinkage levels for FD could be due to drying temperature, which was lower than the glass transition temperature. As occurred in HAD, if the drying temperature is higher than the glass transition temperature of the sample, substantial shrinkage occurs in dried samples [34]. The order of shrinkage value was FD < USV50 < USV60 < USV70 < HAD60 < HAD70 < HAD50. The pictures of dried and fresh samples in Figure 3 also confirmed the results obtained from SEM images. As can be seen in Figure 3, the volume of the samples dried by HAD was lower than that of the others. The FD dried sample was similar to the fresh fruit in terms of total volume and surface characteristics. The lower shrinkage level for USV than HAD could be due to the shorter drying time and less damage to the surface structure [31]. FD showed the lowest shrinkage compared to other drying conditions as reported in the previous studies [19,32,33]. USV 50 ℃ and 60 ℃ showed a similar surface characteristic to FD. The low shrinkage levels for FD could be due to drying temperature, which was lower than the glass transition temperature. As occurred in HAD, if the drying temperature is higher than the glass transition temperature of the sample, substantial shrinkage occurs in dried samples [34]. The order of shrinkage value was FD < USV50 < USV60 < USV70 < HAD60 < HAD70 < HAD50. The pictures of dried and fresh samples in Figure 3 also confirmed the results obtained from SEM images. As can be seen in Figure 3, the volume of the samples dried by HAD was lower than that of the others. The FD dried sample was similar to the fresh fruit in terms of total volume and surface characteristics.  Table 3 showed the effect of the drying method and temperature on the color parameters of the cherry laurel. Drying methods and drying temperature significantly affected L*, a*, and b* value of the  Table 3 showed the effect of the drying method and temperature on the color parameters of the cherry laurel. Drying methods and drying temperature significantly affected L*, a*, and b* value of the samples (p < 0.05). L*, a*, and b* of the fresh samples were found as 29. 23, 9.15, and −4.15, respectively. L* and a* value increased for the samples dried FD while these values decreased for USV and HAD dried samples. Kayacan, Karasu, Akman, Goktas, Doymaz, and Sagdic [12] reported similar results.

The Effect on Cherry Laurel Color
The higher L* and a* value for FD dried samples can be explained by the fact that thermal degradation and browning reaction do not occur in the freeze-drying process at the low temperature [3]. The increase in anthocyanin concentrations after removing water could result in the higher a value obtained by FD. The effect of temperature on color parameters for HAD was also significant (p < 0.05). The longer drying time in the HAD might have led to higher pigment degradation and non-enzymatic browning [35]. The drying temperature of 50 • C showed the lowest L and a value. In terms of color properties, the drying temperature of 60 • C was the best condition for HAD. The combined effect of time and temperature could be an effect on the retention of color parameters. The drying temperature did not significantly affect the color parameters for USV. The ∆E value was calculated to examine total color differences. USV drying conditions and FD showed similar ∆E. However, the ∆E value for FD could be considered positive because color parameters showed an increasing trend. ∆E for HAD and USV showed a decreasing trend and could be regarded as a negative quality index. Color results were in agreement with microstructural properties. HAD, hot air drying; USV, ultrasound-assisted vacuum drying; FD, freeze-drying; Different uppercase letter in the same column indicates differences between samples subjected to different drying methods at the same temperature, FD and fresh sample (p < 0.05). Different lowercase letter in the same column indicates differences between samples subjected to different drying temperature for the same methods FD and fresh sample (p < 0.05). Figure 4 depicted the rehydration kinetics of dried samples at 25 • C. As it was seen, drying methods and drying temperatures showed a different rehydration ratio. In considering the drying method, FD dried samples showed higher water absorption values than that of the others. USV dried samples had more rehydration capacity than HAD dried samples at the same temperature value. The rehydration capacity of the samples was decreased with increased drying temperature at both USV and HAD methods. The significant effect of drying temperature on rehydration capacity was also reported [19,36,37]. The decrease in water absorption capacity with a higher drying temperature of 50-70 • C was reported by Vega-Gálvez, Zura-Bravo, Lemus-Mondaca, Martinez-Monzó, Quispe-Fuentes, Puente, and DiScala [37]. A similar rehydration trend was observed between the samples dried with USV at 70 • C and HAD 50 • C. The higher rehydration capacity obtained from FD for different dried foods was also reported from other studies [19,38]. The higher rehydration capacity for FD could be attributed to lower drying temperature and less damage to the cell-matrix. The spongy texture formed during FD could lead to higher water absorption capacity. During the USV, the application of vacuum and ultrasounds process could lead to a more porous structure compared to HAD and resulted in higher water absorption capacity [33]. The higher rehydration capacity during the USV process can be explained by the higher internal stresses and pore formation. The longer falling rate period and drying time observed in HAD might have resulted in cell disruption and local dry matter accumulation. The increase in rehydration capacity by application of ultrasound for different food types was also reported in other studies [19,39]. ultrasounds process could lead to a more porous structure compared to HAD and resulted in higher water absorption capacity [33]. The higher rehydration capacity during the USV process can be explained by the higher internal stresses and pore formation. The longer falling rate period and drying time observed in HAD might have resulted in cell disruption and local dry matter accumulation. The increase in rehydration capacity by application of ultrasound for different food types was also reported in other studies [19,39]. The Peleg model was applied to describe the effect of drying methods and temperature on the rehydration parameters. Table 4 showed Peleg model parameters, namely M0, K1, and K2. The R 2 values higher than 0.99 indicated that the Peleg model successfully described rehydration kinetics of the samples. K1 and K2 were significantly affected by drying temperature and drying methods. The lowest K1 (8.04), and K2 (0.1) values were obtained from FD dried samples, indicating that sampled dried by FD showed higher rehydration than other samples. The K1 and K2 values were increased by higher drying temperatures. At the same drying temperature, USV showed lower K1 and K2 values than those of HAD, indicating that fruits dried by USV showed higher rehydration capacity than those of HAD.