Antiproliferative, Antimicrobial, and Antifungal Activities of Polyphenol Extracts from Ferocactus Species

: Polyphenols, obtained from natural resources, may possess important pharmacological e ﬀ ects. The polyphenolic proﬁles of the stem extracts of six Ferocactus species (sp.): F. gracilis , F. pottsii , F. herrerae , F. horridus , F. glaucescens , and F. emoryi , were measured using high-performance liquid chromatography (HPLC) with diode-array detection (DAD). Additionally, anticancer, antibacterial, and antifungal activities were examined. Results showed the presence of high to moderate amounts of polyphenols in the extracts (phenolic acids: Protocatechuic acid, 3,4-dihydroxyphenylacetic acid, ca ﬀ eic acid, and vanillic acid; ﬂavonoids: Rutoside and quercitrin). The highest amounts of 3,4-dihydroxyphenylacetic acid were found in F. glaucescens ((132.09 mg 100 g − 1 dry weight (DW)), F. pottsii (75.71 mg 100 g − 1 DW), and F. emoryi (69.14 mg 100 g − 1 DW) while rutoside content was highest in F. glaucescens (107.66 mg 100 g − 1 DW). Maximum antiproliferative activities were observed against HeLa and Jurkat cancer cells, with F. glaucescens , F. emoryi , and F. pottsii showing the highest anticancer activity. Most bacteria were sensitive to Ferocactus sp. stem extracts. Escherichia coli and Staphylococcus aureus were the most sensitive. Excellent antifungal e ﬀ ects were observed against Aspergillus ochraceus and A. niger . However, Penicillium funiculosum , P. ochrochloron , and Candida albicans were relatively resistant. This is the ﬁrst study reporting novel sources of polyphenols in Ferocactus sp. with anticancer and antimicrobial activities. Rutoside was present in highest concentration in F. gracilis. The stem extracts of Ferocactus sp. showed antiproliferative activities against human cancer cell lines, with the highest antiproliferative e ﬀ ects observed against Hela and Jurkat cell lines. The apoptotic assay revealed accumulation of necrotic cells in the early and late stages. The highest antiproliferative activities were found in the stem extracts of F. glaucescens , F. emoryi , and F. pottsii. It was observed that, among the tested bacteria, E. coli and S. aureus were the most sensitive to Ferocactus sp. stem extracts, as demonstrated by low MIC values. Ferocactus sp. stem extracts showed good antifungal properties against selected fungi. Excellent antifungal e ﬀ ects were reported against A. ochraceus and A. niger . In summary, Ferocactus sp. stem extracts could be utilized as a novel source of polyphenols and may be recommended as valuable sources of antimicrobial and anticancer from natural materials. Further investigations should be conducted to evaluate the activity of these extracts against other pathogens. The phytochemical analysis conducted in this study was a partial analysis of the selected compounds in the extract. For ﬁngerprinting purposes, a more sophisticated analysis should be used.


Introduction
Polyphenols (e.g., phenolic acids, lignins, tannins, and flavonoids) represent a wide group of plant secondary metabolites that play a crucial role in counteracting various types of stresses in plants, apart from contributing to the organoleptic properties of plants and plant-derived food [1,2]. Polyphenols are well known for their beneficial effects on human health, due to their antioxidant, anticancer, cardioprotective, anti-inflammatory, and antimicrobial properties [3][4][5][6][7]. In addition, studies have reported that polyphenols could improve some pathological conditions, such as neurodegenerative

Chemical Profiles of the Ferocactus Polyphenolic Extracts
Out of the 21 compounds screened, six polyphenols were identified in the stem extracts of the plants from the Ferocactus sp., using HPLC-DAD. These polyphenols included 4 phenolic acids: Protocatechuic acid, 3,4-dihydroxyphenylacetic acid, caffeic acid, and vanillic acid, and two flavonoids: Rutoside and quercitrin (Table 1 and Figure 2). The two major compounds found in all six plants of the Ferocactus sp. were 3,4-dihydroxyphenylacetic acid and quercitrin. The amounts of 3,4-dihydroxyphenylacetic acid varied from 41.12 to 132.09 mg 100 g −1 dry weight (DW), and the highest amounts were found in F. glaucescens (132.09 ± 15.51 mg 100 g −1 DW), F. pottsii (75.71 ± 7.26 Experimental data regarding the bioactivity of the stem of Ferocactus sp. is limited. In this study, the polyphenolic profiles of six Ferocactus sp. were evaluated (qualitatively and quantitatively) for the first time using high-performance liquid chromatography with diode-array detection (HPLC-DAD) method. The anticancer, antibacterial, and antifungal effects of stem extracts were also explored.

Chemical Profiles of the Ferocactus Polyphenolic Extracts
Out of the 21 compounds screened, six polyphenols were identified in the stem extracts of the plants from the Ferocactus sp., using HPLC-DAD. These polyphenols included 4 phenolic acids: Protocatechuic acid, 3,4-dihydroxyphenylacetic acid, caffeic acid, and vanillic acid, and two flavonoids: Rutoside and quercitrin (Table 1 and Figure 2). The two major compounds found in all six plants of the Ferocactus sp. were 3,4-dihydroxyphenylacetic acid and quercitrin. The amounts of 3,4-dihydroxyphenylacetic acid varied from 41.12 to 132.09 mg 100 g −1 dry weight (DW), and the highest amounts were found in F. glaucescens (132.09 ± 15.51 mg 100 g −1 DW), F. pottsii (75.71 ± 7.26 mg 100 g −1 DW), and F. emoryi (69.14 ± 6.7 mg 100 g −1 DW). The quercitrin content varied from 24.08 to 43.18 mg 100 g −1 DW, and the highest amount was detected in F. gracilis. The rutoside content varied from 7.83 to 107.66 mg 100 g −1 DW, and the highest amount was detected in F. glaucescens. The concentrations of protocatechuic acid, caffeic acid, and vanillic acid in the stem extracts were detected in smaller quantities, ranging from 1.53 to 8.59 mg 100 g −1 DW (Table 1). Based on these results, F. glaucescens can be considered as a rich source of polyphenols (Table 1).

Anticancer Activities of the Ferocactus Polyphenolic Extracts
The stem extracts of the six plants from the Ferocactus sp. showed antiproliferative activities against human cancer cells, as shown in Table 2. The highest antiproliferative activities were observed against HeLa and Jurkat cancer cells. The highest anticancer activity was found in the extracts of F. glaucescens, F. emoryi, and F. pottsii. The anticancer activities of polyphenols were comparable in the F. glaucescens, F. emoryi, and F. pottsii extracts. The antiproliferative effects of 3,4-dihydroxyphenylacetic acid and rutoside against Human Colorectal Adenocarcinoma Cell Line (HT-29) did not show any significant difference compared to vinblastine sulfate. After 48 h of treatment with different

Anticancer Activities of the Ferocactus Polyphenolic Extracts
The stem extracts of the six plants from the Ferocactus sp. showed antiproliferative activities against human cancer cells, as shown in Table 2. The highest antiproliferative activities were observed against HeLa and Jurkat cancer cells. The highest anticancer activity was found in the extracts of F. glaucescens, F. emoryi, and F. pottsii. The anticancer activities of polyphenols were comparable in the F. glaucescens, F. emoryi, and F. pottsii extracts. The antiproliferative effects of 3,4-dihydroxy-phenylacetic acid and rutoside against Human Colorectal Adenocarcinoma Cell Line (HT-29) did not show any significant difference compared to vinblastine sulfate. After 48 h of treatment with different extracts, the apoptotic assay showed high accumulation of necrosis in the early and late apoptotic cells when compared to the control (Figure 3). Treatment with 2-3,4-dihydroxyphenylacetic acid and rutoside showed similar accumulation of necrotic cells, as seen in treatment with the stem extracts of F. glaucescens, F. emoryi, and F. pottsii. Table 2. In vitro antiproliferative activity inhibitory concentration (IC 50 (µg/mL)) of Ferocactus sp. stem extracts (mg mL −1 ) and the main identified compounds on different cancer cell lines. Values are presented as means of three replicates. Different letters within a column indicate significant differences at p ≤ 0.05.

Antibacterial Activities of the Ferocactus Polyphenolic Extracts
The stem extracts of the different Ferocactus sp. showed remarkable antibacterial activities against Pseudomonas aeruginosa, Bacillus cereus, Listeria monocytogenes, Escherichia coli, Mariniluteicoccus flavus, and Staphylococcus aureus, as shown in Table 3. The highest antibacterial activities were observed in the stem extracts of F. glaucescens, F. emoryi, and F. pottsii. Polyphenol standards of 3,4-

Antibacterial Activities of the Ferocactus Polyphenolic Extracts
The stem extracts of the different Ferocactus sp. showed remarkable antibacterial activities against Pseudomonas aeruginosa, Bacillus cereus, Listeria monocytogenes, Escherichia coli, Mariniluteicoccus flavus, and Staphylococcus aureus, as shown in Table 3. The highest antibacterial activities were observed in the stem extracts of F. glaucescens, F. emoryi, and F. pottsii. Polyphenol standards of 3,4-dihydroxyphenylacetic acid, rutoside, and quercitrin showed comparable or higher activities than those of the extracts. Most bacteria were sensitive to different Ferocactus sp. stem extracts; especially, E. coli and S. aureus were found to be most sensitive, as demonstrated by low minimum inhibitor concentration (MIC) values.

Antifungal Activities of the Ferocactus Polyphenolic Extracts
Ferocactus stem extracts showed good antifungal properties against the selected fungi, as shown in Table 4. The MIC and minimum fungicidal concentration (MFC) values were generally low for all the Ferocactus sp. Excellent antifungal effects were observed against Aspergillus ochraceus and A. niger. However, Penicillium funiculosum, P. ochrochloron, and Candida albicans were relatively more resistant. The activities of the extracts matched those of the commercial reagent ketoconazole (KTZ). The antifungal activities of phenolic standards, 3,4-dihydroxyphenylacetic acid, rutoside, and quercitrin, were comparable to those of F. glaucescens, F. emoryi, and F. pottsii extracts.

Discussion
The qualitative and quantitative HPLC-DAD analyses of the stem extracts of six Ferocactus sp., F. gracilis, F. pottsii, F. herrerae, F. horridus, F. glaucescens, and F. emoryi, indicated the presence of six polyphenolic compounds, namely protocatechuic acid, 3,4-dihydroxyphenylacetic acid, caffeic acid, vanillic acid, rutoside, and quercitrin. The highest concentrations of detected polyphenols were confirmed in F. glaucescens ( Table 1). The major polyphenols found in high concentrations in all the studied Ferocactus sp. were 3,4-dihydroxyphenylacetic acid, rutoside, and quercitrin. Protocatechuic acid, caffeic acid, and vanillic acid were detected in smaller quantities, ranging from 1.53 to 8.59 mg 100 g −1 DW ( Table 1). The highest concentration of 3,4-dihydroxyphenylacetic acid was found in F. glaucescens (132.09 mg 100 g −1 DW) and this value was several times higher than that found in the other species (Table 1). The abundant availability of 3,4-dihydroxyphenylacetic acid in a natural plant source is not common. Dihydroxyphenylacetic acid has been reported to be found in much lower concentrations in Eucalyptus globulus bark [21]. On the other hand, quercitrin is not as rare as 3,4-dihydroxyphenylacetic acid. It is commonly found in vegetables and fruits [22]. Similarly, rutoside is common in foods and has important therapeutic potential [23].
The stem extracts of different Ferocactus sp. showed obvious antiproliferative effects against various cancer cells, especially against HeLa and Jurkat cancer cells. The extracts of F. glaucescens, F. emoryi, and F. pottsii showed highest antiproliferative effects. This could be attributed to the abundant presence of specific bioactive polyphenol compounds, such as 3,4-dihydroxyphenylacetic acid, rutoside, quercitrin, and protocatechuic acid in these extracts. The 3,4-Dihydroxyphenylacetic acid was found to have apoptotic effect on human colon adenocarcinoma cells [24]. The extracts of F. gracilis, F. herrerae, and F. horridus showed moderate antiproliferative activities against most cancer cells. Previous investigations on other genera of Cactaceae, such as the famous genus Opuntia sp., revealed antiproliferative activities of the plant juice against HT-29 cells [25]. Cell cycle arrest in the apoptotic assay at the G1, G2/M, and S was reported. These effects were attributed to the phytochemical composition (betacyanins, isorhamnetin derivatives, and ferulic acid) of these plants. In Cereus peruvianus Mill (Cactaceae), antiproliferative activity was observed, owing to a high composition of unsaturated fatty acids [26].
The apoptotic activity of 3,4-dihydroxybenzoic acid (protocatechuic acid) has been reported in human gastric carcinoma cells [27] by the induction of JNK/p38 activity in protocatechuic acid (PCA)-responsive cell lines. Rutoside (3,3 ,4 ,5,7-pentahydroxyflavone-3-rhamnoglucoside) is a flavonol, commonly found in plants, and has cytoprotective, antioxidant, and anticarcinogenic activities against several cancer cell types [23]. Rutoside induces G2/M cell cycle arrest and activates apoptosis in human neuroblastoma cancer cells [28]. In another study, rutoside acted against cancer cells through antioxidant mechanism [29]. Quercitrin has shown strong anticancer activities owing to apoptosis-inducing effects [30]. Similar to earlier studies, accumulation of necrotic cells in the cell cycle was observed in this study.
Antibacterial effects were observed in the stem extracts of the six Ferocactus sp. The highest antibacterial activities were observed in the stem extracts of F. glaucescens, F. emoryi, and F. pottsii. Further, polyphenol standards of 3,4-dihydroxyphenylacetic acid, rutoside, and quercitrin showed comparable or higher values than those observed in the extracts, thus implying that these polyphenols were responsible for the antibacterial effects. Rutoside has been implicated in antibacterial activities against B. cereus and Salmonella enteritidis [31] and S. aureus [32]. Quercitrin and other flavonoids have also shown antibacterial activities against several bacteria [33]. Polyphenols, in general, are known for their antibacterial activities [34]. Furthermore, Ferocactus stem extracts showed good antifungal properties. Excellent antifungal effects were observed against Aspergillus ochraceus and A. niger. However, the antifungal activities were lower against Penicillium funiculosum, P. ochrochloron, and Candida albicans. Several reports have indicated that rutoside, quercitrin, protocatechuic acid, and vanillic acid have antifungal activities [3,4,35,36].

Preparation of Polyphenolic Extracts
The stems of Ferocactus sp. (F. gracilis H.E.Gates, F. pottsii (Salm-Dyck) Backeb, F. herrerae J.G.Ortega, F. horridus Britton and Rose, F. glaucescens (DC) Britton and Rose, F. emoryi Engelm, Orcutt) were sampled from commercial nurseries in Alexandria, Egypt, and identified by Hosam Elansary. Voucher specimens were deposited at Alexandria (Hosam 0001020-1027). The stem samples were dried by lyophilization (Labconco, USA) and then powdered. Three replicates of the dried samples (0.5 g DW each) were put in 15 mL tubes and subjected to extraction with 10 mL methanol (Chempur, Poland) by sonication (2 × 30 min at 30 • C) in an ultrasonic bath (Sonic-2, POLSONIC, ultrasonic power 2 × 100 W, 40 kHz, water bath dimensions 150 × 135 × 100 mm). The extracts were filtered using Whatman paper and left in crystallizers to evaporate methanol at room temperature (25 • C). The dry residue was dissolved in 1 mL methanol (Merck, HPLC grade purity) [37]. Obtained extracts were filtered through sterilized syringe filters (0.22 µm, Millex ® GP, Millipore, Burlington, Mississippi, USA) prior to HPLC analyses. The samples were stored for future bioassays (−80 • C). For bioassays, methanol was totally removed by evaporation using a rotary evaporator. Analytical/HPLC grade chemicals were used (Sigma Aldrich, Germany) for the bioassays. The bacterial and fungal cultures were obtained from the Faculty of Agriculture, Alexandria, Egypt.
The percentage inhibition of antiproliferative activity (IAA) was calculated in triplicates: where (AB 570nm ) C and (AB 570nm ) s are Abs.570 nm of control and sample, respectively.

Apoptotic Assay
The inhibitory concentration IC 30 and IC 50 values were determined in the apoptotic cell population using a flow cytometry (FAC Scan, Becton Dickinson, Iowa, USA) [3,17,42].

Statistical Analyses
The least significant difference (LSD) was computed using the SPSS software (version 22.0). Experiments were repeated twice. The standard deviation (SD) of means of three replicates was used.

Conclusions
To our knowledge, this is the first report that explored the presence of polyphenols in the stem extracts of six Ferocactus sp., and investigated their respective bioactivities as anticancer, antibacterial, and antifungal raw materials. Six polyphenols were identified (phenolic acids: Protocatechuic acid, 3,4-dihydroxyphenylacetic acid, caffeic acid, and vanillic acid and flavonoids: Rutoside and quercitrin). The major compounds found in all the six species were 3,4-dihydroxyphenylacetic acid and quercitrin.
Rutoside was present in highest concentration in F. gracilis. The stem extracts of Ferocactus sp. showed antiproliferative activities against human cancer cell lines, with the highest antiproliferative effects observed against Hela and Jurkat cell lines. The apoptotic assay revealed accumulation of necrotic cells in the early and late stages. The highest antiproliferative activities were found in the stem extracts of F. glaucescens, F. emoryi, and F. pottsii. It was observed that, among the tested bacteria, E. coli and S. aureus were the most sensitive to Ferocactus sp. stem extracts, as demonstrated by low MIC values. Ferocactus sp. stem extracts showed good antifungal properties against selected fungi. Excellent antifungal effects were reported against A. ochraceus and A. niger. In summary, Ferocactus sp. stem extracts could be utilized as a novel source of polyphenols and may be recommended as valuable sources of antimicrobial and anticancer from natural materials. Further investigations should be conducted to evaluate the activity of these extracts against other pathogens. The phytochemical analysis conducted in this study was a partial analysis of the selected compounds in the extract. For fingerprinting purposes, a more sophisticated analysis should be used.