Ciprofloxacin Uptake from an Aqueous Solution via Adsorption with K 2 CO 3 -Activated Biochar Derived from Brewing Industry Bagasse

: Ciprofloxacin (CPX), an antibiotic considered as an emerging contaminant, needs to be removed from aquatic environments. This work investigates the adsorption of CPX on K 2 CO 3 -activated biochar (AB). The biochar was obtained via the pyrolysis of barley bagasse from the brewing industry, and then it was activated with 2M of K 2 CO 3 . The activated biochar was characterised using FTIR spectroscopy and a pH PZC assay. Batch adsorption tests were performed to study the influence of pH and temperature on CPX sorption and to obtain kinetic and equilibrium data. The adsorption of CPX on AB was favoured by increasing the temperature from 10 ◦ C to 55 ◦ C, demonstrating the endothermic nature of the process. The level of CPX removal after 24 h of contact and at pH 3.5 was 82% of that obtained when equilibrium was reached. The kinetic study showed that the adsorption is well described by the Elovich and the Bangham kinetic models. The adsorption is favourable, and the best fits to the experimental equilibrium data were obtained with the Freundlich, Redlich–Peterson and Sips isotherms. In an acidic solution (pH = 3.5) and at 25 ◦ C, the maximum CPX adsorption capacity of AB was ≈ 105 mg · g − 1 , comparable to that reported for other adsorbents.


Introduction
Until a few years ago (at the end of the 20th century), there was no particular interest in the pollution potential of the presence of pharmaceuticals and personal care products in general, and of antibiotics in particular, in water streams.These compounds are present in the effluents of pharmaceutical manufacturing plants, hospitals, farms and households.Most are persistent and poorly biodegradable.Because they cannot be completely eliminated in conventional wastewater treatment plants, concern about their occurrence and fate in the environment has increased over the last few decades.In addition, antibiotics are designed to act on biological targets, so they can be very harmful to microorganisms and interfere with the proper functioning of wastewater treatment plants [1][2][3].
Ciprofloxacin (CPX) is a synthetic fluoroquinolone antibiotic widely used in human and veterinary medicine.Concentrations of ciprofloxacin found in water and wastewater are typically <1 µg•L −1 [4].Several studies have reported concentrations of 3-87 µg•L −1 in hospital wastewater, and up to 50 mg•L −1 in wastewater from pharmaceutical production facilities [5].Their presence in the environment can have adverse effects on water quality and induce the growth of antibiotic-resistant bacteria [4,6].Therefore, wastewater treatment is necessary in order to remove CPX.
Various treatment methods have been used to remove CPX from aqueous effluents: chemical and electrochemical oxidation, photocatalytic and enzymatic degradation, membrane separation and adsorption [4,7].Adsorption has proven to be an effective and promising method due to its simple design and operation, its low cost, the possibility of adsorbent regeneration and its ability to remove low concentrations of micropollutants [8].The most commonly used adsorbent is activated carbon, although the use of a variety of alternative adsorbents is being investigated.Malakootian et al. (2021) [9] reviewed the removal of CPX via adsorption.Some of the alternative adsorbents studied include zeolites, carbon nanotubes, graphene, nanostructured hybrid materials, clays, magnetic adsorbents, nanoparticles, etc. [1,4,6,[9][10][11][12].
Due to the high cost of activated carbon, the use of biochar as a solid adsorbent has been of great interest in recent years [13].Biochar is a carbon-rich material obtained by heating biomass at relatively low temperatures (<700 • C) in an oxygen-poor atmosphere.Biochar is known for its ability to act as a remediator of contaminants, playing an important role in climate change mitigation and bioenergy production [14].Its high specific surface area, porous structure, surface rich in functional groups and its mineral components make biochar a very good adsorbent for the removal of pollutants present in aqueous solutions [15][16][17][18][19].The major mechanisms involved in pharmaceutical pollutant adsorption are pore filling, metal complexing, precipitation, hydrogen bonding, ion exchange, cation bridging and hydrophobic partition [20,21].The behaviour of biochar as an adsorbent depends on the type of raw material and the method used to obtain it (mainly the temperature and residence time) [22,23].
The adsorption performance of raw biochar is limited.This material, typically derived from renewable and low-cost agricultural biomass and solid wastes, can be physically or chemically activated to improve its adsorption properties.As a result, the performance of activated biochar in removing pollutants is comparable to that of commercial activated carbon.The activation of biochar can be carried out via physical methods using steam or gas (CO 2 , N 2 , NH 3 , air, O 2 or their mixtures).Chemical activation can be conducted through treatments with acids (HCl, HNO 3 , H 2 SO 4 or H 3 PO 4 ), alkalis (NaOH, KOH or K 2 CO 3 ) or oxidants (H 2 O 2 or KMnO 4 ) [22].Several studies have investigated CPX adsorption on raw and activated biochar prepared from different feedstocks: bamboo chips [7], cassava dregs [15], desilicated rice husks [24], used tea leaves [16], potato stems and leaves [17], rice husks [25], rabbit manure [26], municipal solid waste [27], sewage sludge [28], banana peels [29], kidney bean peels, tangerine peels, snowberry, borage and red pepper seeds [30], pumpkin peels [31], etc.
Various by-products and residues are generated during the production of beer.The most abundant by-product is spent grains (bagasse), which is produced after the pressing and filtering of the must from the saccharification of the malted barley grains.It is estimated that the production of 1000 L of beer produces 200 kg of wet spent grains (containing 70-80% water) [32].The main use of this by-product is its use as animal feed, due to its high protein and fibre content.Spent grains are a carbonaceous material, rich in cellulose, hemicellulose and lignin (together accounting for about 72% (w/w) of the dry composition of spent grain) containing large amounts of sugars and numerous phenolic compounds and amino acids [33].This material has been used as a precursor for various adsorbents for the removal of various drugs in aqueous solutions [34][35][36].In this work, bagasse from the brewing industry was used as a feedstock to produce activated biochar (AB) via chemical activation with K 2 CO 3 .The hydrolysis of this activation agent produces KOH, and compared to this, potassium carbonate has the advantage of being less corrosive, easier to store and more environmentally friendly.Furthermore, previous studies have shown that this activation agent is very effective in creating porosity in hydrochar, resulting in materials with large surface areas (>2000 m 2 •g −1 ) and a tenable pore size distribution [37].
The main objective of this research Is to study the uptake of CPX from aqueous solutions through sorption on AB.The influence of pH and temperature on adsorption uptake was analysed.Sorption kinetics and equilibrium isotherms were determined, and the sorption mechanism was discussed.Considering the interest of the European biochar certificate in the sustainable use of biochar in Europe, this study can contribute to providing information on the possibility of using waste from the brewing industry as a precursor of activated biochar for wastewater treatment, specifically for the removal of CPX, an emerging pollutant commonly found in wastewater.

Activated Biochar Preparation
The adsorbent used in this work was biochar activated with potassium carbonate.The biochar was obtained from bagasse, a by-product of the brewing industry.The bagasse, collected from an industry located in the Region of Murcia (Spain), was first dried at 60 • C-70 • C in an oven, and then crushed and sieved, to obtain particles with a size between 1.0 mm and 2.5 mm.This fraction size was pyrolysed in a horizontal rotary quartz furnace (Nabertherm) under an inert argon flow, according to the following steps: 1.
Heating from room temperature to 105 • C in 15 min; 2.
Cooling to room temperature; 6.
The argon flow was 50 L•h −1 in the 1st, 2nd and 5th stages, and 150 L•h −1 in the 3rd and 4th stages.
The pyrolysis conditions were set, taking into account those reported in the literature for similar studies.Thus, the treatment at 400 • C with a ramp of 5 • C•min −1 is common in the production of biochar from different types of biomass, as these conditions fall within the range used to favour the production of biochar over the production of biooil and gasification [38,39].Furthermore, these particle size and pyrolysis conditions have previously been used to produce biochar for soil remediation and have shown good performance in terms of biochar production [40].
The resulting biochar was then crushed, sieved and activated via an alkaline treatment with K 2 CO 3 .A total of 5.0 g of biochar (particle size between 0.15 mm and 0.5 mm) was suspended in 100 mL of a 2 mol•L −1 K 2 CO 3 solution with continuous stirring.After 24 h, the suspension was centrifuged, and the solid was dried at 105 • C, placed in lidded porcelain crucibles and heated in a muffle furnace at 800 • C for 3 h.Finally, the activated biochar was repeatedly washed with distilled water to a stable pH (pH ≈ 7) and dried at 60 • C-70 • C.These impregnation and carbonisation conditions are within the normal range used for the alkaline activation of other biochars derived from other low-cost materials [41,42].At high activation temperatures (600 • C-800 • C), potassium carbonate catalyses the gasification of cellulose, which is more complete at higher temperatures.Thus, the use of potassium carbonate as an activation agent at high temperatures produces biochar with high porosity and a high specific surface area, which facilitates the diffusion of adsorbates and increases their adsorption capacity [42,43].

Activated Biochar Characterization
Fourier Transform Infrared Spectroscopy (FTIR) and the point of zero charge were used to characterize the AB sorbent.
FTIR is a commonly used technique to identify surface functional groups on sorbents.For this purpose, FTIR spectra (Perkin Elmer 16F PC) of AB samples were obtained before and after CPX adsorption.The samples were prepared as KBr discs, and the spectra were recorded in the range of 650-4000 cm −1 .
The electrical charge on the surface of a sorbent is zero at the point of zero charge.This parameter is usually considered as the pH (pH pzc ) at which a solid immersed in an electrolyte has a net zero electrical charge on the surface.
The point of zero charge was determined using two classical methods: the immersion technique and mass titrations.
In the immersion technique [44], 20 mL of a 0.01 M NaCl solution were placed in different flasks, and the pH was adjusted to values between 3 and 9.5 by adding NaOH or HCl solutions.Then, 20 mg of AB were added to each flask, and the suspensions were shaken for 24 h until the equilibrium pH was reached (pH final ).Blank tests were made without AB samples and their pH was measured after 24 h, referred to as pH initial .The pH pzc value is the point where the pH initial = pH final in the plot of pH final vs. pH initial .
In the mass titration method [45], 5 mL of a 0.01 M NaCl solution were added to each of several flasks.Different amounts of solid AB were added to each flask to obtain solid suspensions with concentrations between 2 and 80 g•L −1 .After 24 h of agitation, the pH of the suspensions was measured.Plotting the equilibrium pH versus the sorbent concentration achieves a plateau corresponding to the pH pzc value.

CPX Solutions' Preparation and Analysis
Ciprofloxacin (C 17 H 18 FN 3 O 3 ) with >98% purity was supplied by Sigma-Aldrich.The molecular weight of CPX is 331.35 g•mol −1 , and its molecule has two dissociation constants: pK a1 = 5.9 ± 0.15 corresponding to the carboxyl group, and pK a2 = 8.89 ± 0.11 for the amine group in the piperazine moiety.As a function of pH, the CPX molecule exists in different ionic forms: the cationic form predominates when the pH is below 5.9, the zwitterionic form predominates when the pH is between 5.9 and 8.89 and the anionic form predominates when the pH is above 8.89 [1,24,46] (Supplementary Figure S1).
A CPX stock solution (1000 mg•L −1 ) was prepared by dissolving 1 g of CPX in 1 L of 0.1 M HCl.Working solutions of different CPX concentrations were obtained by diluting the CPX stock solution with distilled water.The pH of the solutions was adjusted through the addition of 0.1 M NaOH or HNO 3 solutions, if necessary.The concentration of CPX in the solutions was measured via UV spectrophotometry (Shimadzu UV-1603 UV-visible spectrophotometer).The wavelength at which the maximum absorbance occurred in the CPX solutions was pH-dependent: λ max = 277.5 nm at acidic pH and λ max = 272 nm at basic pH (absorption spectra of CPX solutions at acidic or basic pH are shown in the Supplementary Figure S2).Two calibration curves were obtained, one at λ = 277.5 nm for acidic solutions and other one at λ = 272 nm for basic solutions, both with regression coefficients greater than 0.999.From each of these calibration curves, the limit of detection (LOD) and limit of quantitation (LOQ) were obtained according to IUPAC and ACS recommendations, respectively [47,48].The limits obtained under acidic conditions were LOD = 0.70 mg•L −1 and LOQ = 2.34 mg•L −1 , and those obtained under basic conditions were LOD = 0.72 mg•L −1 and LOQ = 2.40 mg•L −1 .

Adsorption Experiments
To determine the influence of pH and temperature on the sorption process, and to study the kinetic and the equilibrium of the CPX adsorption, batch adsorption experiments were carried out, at a constant temperature and pH, in magnetically stirred glass flasks.Typically, known amounts of sorbent were placed in contact with a CPX solution of a known concentration.The pH of the suspension was maintained at the desired value by adding dilute solutions of HNO 3 or NaOH.At specified times, suspension samples were collected and centrifuged, and the supernatant was analysed for CPX determination.The amount of CPX adsorbed per unit of adsorbent, q (mg CPX•g −1 ), was obtained from Equation (1).
where C 0 and C are the initial CPX concentration in the solution and that at time t (mg•L −1 ), respectively; V 0 and V are the initial solution volume and that at time t (L); and m is the mass (g) of sorbent used.
The tests were carried out in triplicate with a sorbent dose of 0.5 g•L −1 in all cases.Blank tests were also performed by contacting the solid adsorbent with distilled water, which showed that the absorbance of the solutions obtained was negligible at λ = 277.5 nm and 272 nm.
The influence of pH on CPX adsorption was investigated at 25 • C by mixing 0.025 g of the sorbent with 50 mL of the 300 mg•L −1 CPX solution for 72 h at a pH adjusted between 2 and 10.5.Blank tests were performed without the sorbent at the same pH values.
The effect of temperature was studied at pH 3.5 by contacting 0.025 g of sorbent with 50 mL of the 300 mg•L −1 CPX solution for 72 h.The tests were carried out at 10 The adsorption kinetic was determined at 25 • C and pH 3.5.The solid adsorbent (0.1 g) was added to 200 mL of the 300 mg•L −1 CPX solution, and suspension samples were periodically taken and analysed.Different kinetic models (pseudo-first-and pseudo-secondorder, Elovich, intraparticle diffusion and Bangham) were used to obtain information on the adsorption mechanism (Table 1) [49].

Kinetic Model Equation Parameters
Pseudo-first order q e , amount of adsorbate sorbed at equilibrium (mg•g −1 ) k' 2 , adsorption rate constant (g related to the extend of surface coverage and activation energy for chemisorption (g•mg −1 ) The adsorption isotherm was obtained at 25 • C and pH 3.5.A total of 0.025 g of the sorbent and 50 mL of the CPX solutions with concentrations ranging from 25 mg•L −1 to 1000 mg•L −1 were mixed in glass flasks until equilibrium was reached (≈80 h).The Langmuir, Freundlich, Sips, Redlich-Peterson and Toth isotherm models were used to understand the adsorption interactions (Table 2) [50].

Isotherm Model Equation Parameter
Langmuir q e = 1/T T q mT , maximum sorption capacity (mg•g −1 ) K T , constant that characterize the adsorptive potential ((L•g −1 ) T T ) T T , Toth parameter related to the heterogeneity of the adsorbent (dimensionless) The kinetic models and the equilibrium isotherms were fitted to the corresponding experimental data using non-linear regression with the Solver add-in of Microsoft Excel, minimising the ARE function (average relative error, %) (Equation (2)).
where p is the number of data, q exp is the experimental amount of CPX adsorbed per unit of adsorbent and q cal is the amount of CPX adsorbed per unit of adsorbent provided by the model.

Activated Biochar Characterization and Adsorption Mechanism
Figure 1 shows the FTIR spectra of the adsorbent before and after CPX adsorption.Both spectra are very similar, and the same spectral peaks can be observed.No significant changes in the peak profile can be detected after CPX adsorption.The high number of adsorption peaks highlights the complexity of the activated biochar.An intense absorption peak at 3434 cm −1 can be clearly identified, corresponding to the stretching vibrations of the O-H of the free hydroxyl groups (alcohols and phenols) and the bonded OH bands of carboxylic acids.Also, the presence of adsorbed water molecules from atmospheric humidity may influence the intensity and amplitude of this band [51,52].
qmT, maximum sorption capacity (mg•g −1 ) KT, constant that characterize the adsorptive potential ((L•g −1 ) T T) TT, Toth parameter related to the heterogeneity of the adsorbent (dimensionless)

Activated Biochar Characterization and Adsorption Mechanism
Figure 1 shows the FTIR spectra of the adsorbent before and after CPX adsorption.Both spectra are very similar, and the same spectral peaks can be observed.No significant changes in the peak profile can be detected after CPX adsorption.The high number of adsorption peaks highlights the complexity of the activated biochar.An intense absorption peak at 3434 cm −1 can be clearly identified, corresponding to the stretching vibrations of the O-H of the free hydroxyl groups (alcohols and phenols) and the bonded OH bands of carboxylic acids.Also, the presence of adsorbed water molecules from atmospheric humidity may influence the intensity and amplitude of this band [51,52].The peak at 2923 cm −1 can be attributed to the asymmetric stretching of C-H in aliphatic groups.The peak at 2853 cm −1 can be attributed to the symmetric stretching of C-H in aliphatic groups, and to the methoxy group (CH 3 -O).The band at 1631 cm −1 shows the asymmetric stretching of COO-, the C=C stretching that can be attributed to the presence of aromatic rings, and the bending vibration of the interlayer water molecules [52].The peak at 1384 cm −1 corresponds to the symmetric stretching of COO-or S=O in sulphonyl and sulphonate.The peak at 1115 cm −1 , in the range of 1050-1150 cm −1 , can be attributed to the C-O stretching vibration in carboxylic acids, ethers and alcohols [10,16,53].Therefore, biochar presents an abundant number of surface functional groups such as hydroxyl, methyl, carbonyl and carboxyl groups; the C=C bonds and C-H groups of the aromatic rings may be implicated in the adsorption of CPX by AB.
The experimental values corresponding to the mass titration and immersion techniques are shown in Figure 2. The pH pzc obtained by these two methods was ≈8.1, slightly basic, according with the activation method used (with K 2 CO 3 ).The solid surface therefore has a basic character.
in aliphatic groups, and to the methoxy group (CH3-O).The band at 1631 cm shows the asymmetric stretching of COO-, the C=C stretching that can be attributed to the presence of aromatic rings, and the bending vibration of the interlayer water molecules [52].The peak at 1384 cm −1 corresponds to the symmetric stretching of COO-or S=O in sulphonyl and sulphonate.The peak at 1115 cm −1 , in the range of 1050-1150 cm −1 , can be attributed to the C-O stretching vibration in carboxylic acids, ethers and alcohols [10,16,53].Therefore, biochar presents an abundant number of surface functional groups such as hydroxyl, methyl, carbonyl and carboxyl groups; the C=C bonds and C-H groups of the aromatic rings may be implicated in the adsorption of CPX by AB.
The experimental values corresponding to the mass titration and immersion techniques are shown in Figure 2. The pHpzc obtained by these two methods was ≈ 8.1, slightly basic, according with the activation method used (with K2CO3).The solid surface therefore has a basic character.The adsorption of organic pollutants onto biochar can involve different mechanisms such as pore filling, hydrophobic interaction, partitioning, hydrogen bonds, van der Waals forces, electrostatic interaction and π-π interactions [8,21].The mechanisms implicated in the adsorption of CPX on biochar are complex due to the chemical structure of the CPX molecule, with several aromatic rings and ionisable functional groups, and the heterogeneous nature of the adsorbent solid.Nevertheless, it can be suggested that several possible mechanisms are involved: electrostatic attraction, hydrogen bonding and π-π interactions.
Electrostatic interactions can occur between the functional groups of the activated biochar and the CPX molecules.In this sense, the solution pH plays an important role, since both the ionic form of CPX and the charge on the AB surface are affected by this parameter.The tests were carried out at pH 3.5 to avoid CPX removal via precipitation.At this pH, which is lower than pHpzc, the sorbent surface is positively charged and the predominant ionic form of CPX is the cationic one.Therefore, the contribution of this The adsorption of organic pollutants onto biochar can involve different mechanisms such as pore filling, hydrophobic interaction, partitioning, hydrogen bonds, van der Waals forces, electrostatic interaction and π-π interactions [8,21].The mechanisms implicated in the adsorption of CPX on biochar are complex due to the chemical structure of the CPX molecule, with several aromatic rings and ionisable functional groups, and the heterogeneous nature of the adsorbent solid.Nevertheless, it can be suggested that several possible mechanisms are involved: electrostatic attraction, hydrogen bonding and π-π interactions.
Electrostatic interactions can occur between the functional groups of the activated biochar and the CPX molecules.In this sense, the solution pH plays an important role, since both the ionic form of CPX and the charge on the AB surface are affected by this parameter.The tests were carried out at pH 3.5 to avoid CPX removal via precipitation.At this pH, which is lower than pH pzc , the sorbent surface is positively charged and the predominant ionic form of CPX is the cationic one.Therefore, the contribution of this mechanism to the overall adsorption process should be slight, and there must be other different mechanisms than electrostatic attraction involved in CPX adsorption by AB [45].
Adsorption may also involve hydrogen-bond interactions.The CPX molecule contains several moieties such as aromatic rings, as well as nitrogen, fluorine and oxygen atoms that may interact through H-bonds with the aromatic rings and other groups (such as -OH and COOH) on the surface of AB.
Taking into account the presence of phenolic groups and aromatic rings on the AB surface, it can be suggested that there may be π-π interactions implicated in the adsorption process.This type of interaction may contribute significantly to the adsorption of organic pollutants possessing aromatic rings.The π-π interaction occurs between electron-poor and electron-rich structures.The CPX molecule possesses aromatic rings connected to O, N and F atoms.These atoms are highly electronegative and have a high electron-holding ability.
Processes 2024, 12, 199 8 of 16 This will make the aromatic rings electron-deficient, acting as electron acceptors.The hydroxyl groups and the C=C bonding of the aromatic rings on the adsorbent surface make them electron donors.Consequently, an electron donor/acceptor adsorption mechanism based on the non-uniform distribution of electrons between the adsorbate molecule and the functional groups of the adsorbent may contribute to the adsorption of CPX on AB.
So, it can be hypothesised that hydrogen bonding and π-π interactions may be involved in the adsorption of CPX onto AB.

Effect of pH and Temperature on CPX Adsorption
The amount of CPX adsorbed per unit of adsorbent is usually significantly affected by the pH of the solution, which affects the chemical form of the adsorbate (anionic, cationic or molecular forms) and the surface properties (surface functional groups, charge and charge density) of the adsorbent solid.Figure 3 shows the removal efficiency of CPX vs. the pH of the solution at equilibrium in tests carried out with and without (blank trials) the solid adsorbent.
tion process.This type of interaction may contribute significantly to the adsorption of organic pollutants possessing aromatic rings.The π-π interaction occurs between electronpoor and electron-rich structures.The CPX molecule possesses aromatic rings connected to O, N and F atoms.These atoms are highly electronegative and have a high electronholding ability.This will make the aromatic rings electron-deficient, acting as electron acceptors.The hydroxyl groups and the C=C bonding of the aromatic rings on the adsorbent surface make them electron donors.Consequently, an electron donor/acceptor adsorption mechanism based on the non-uniform distribution of electrons between the adsorbate molecule and the functional groups of the adsorbent may contribute to the adsorption of CPX on AB.
So, it can be hypothesised that hydrogen bonding and π-π interactions may be involved in the adsorption of CPX onto AB.

Effect of pH and Temperature on CPX Adsorption
The amount of CPX adsorbed per unit of adsorbent is usually significantly affected by the pH of the solution, which affects the chemical form of the adsorbate (anionic, cationic or molecular forms) and the surface properties (surface functional groups, charge and charge density) of the adsorbent solid.Figure 3 shows the removal efficiency of CPX vs. the pH of the solution at equilibrium in tests carried out with and without (blank trials) the solid adsorbent.In the assays performed at pH 6 to 10, turbidity was observed in the solutions, indicating that CPX precipitation occurred.For this reason, blank assays (without adsorbent) were performed.It was observed that the percentage of CPX removal at pH 7 to 9 was In the assays performed at pH 6 to 10, turbidity was observed in the solutions, indicating that CPX precipitation occurred.For this reason, blank assays (without adsorbent) were performed.It was observed that the percentage of CPX removal at pH 7 to 9 was very similar in the assays carried out with and without the adsorbent.Therefore, the increase in CPX removal observed at these pH values was mainly due to precipitation rather than adsorption.
As mentioned above, CPX can exist in different forms depending on the pH value.At pH values less than 5.9, the dominant form in the solution is cationic CPX (CPX + ).At pH values above 8.9, the predominant form is anionic CPX (CPX − ).The solubilities of these net-charged species (CPX + and CPX − ) are high, being higher at pH values below 5 (>30 g•L −1 ).At pH values between 5.9 and 8.9, the zwitterionic form predominates, and the solubility of this neutral species is very low (<0.12g•L−1 ) [1,24,46].Therefore, the following experiments were performed at pH 3.5 in order to minimise the removal of CPX via precipitation.
The amount of CPX adsorbed per unit of adsorbent was obtained at different temperatures.It was found that the q e values increased with increasing temperatures (34.6 mg•g −1 at 10 • C, 41.4 mg•g −1 at 25 • C, 89.3 mg•g −1 at 40 • C and 109.9 mg•g −1 at 55 • C).According to these results, it can be stated that adsorption was favoured as the temperature increased, showing the endothermicity of this process.This fact is in agreement with the investigations of CPX adsorption on different sorbents reviewed by Malakootian et al. (2021) [9].This process is endothermic in 66% of the papers investigating the thermodynamics of CPX adsorption and in all those where the sorbent used is biochar: rabbit manure biochar [26], a magnetic biochar-based manganese oxide composite [18], potassium hydroxide-modified biochar derived from potato stems and leaves [17] and a coating of magnetic biochar with humic acid [54].However, CPX adsorption was exothermic on biochar prepared from cassava dregs [15] and both exothermic and endothermic on biochar obtained from used tea leaves [16].

Adsorption Kinetics
The study of the adsorption kinetics is of great importance for the prediction of the rate at which CPX will be removed and for the design of the appropriate adsorption equipment.Figure 4 shows the experimental values of CPX adsorbed per gram of adsorbent (q t ) vs. contact time.As can be seen, the adsorption process takes place in several steps.Initially, in the first step, the q t values increase sharply with the contact time because the system is far from equilibrium and many binding sites are available on the external surface and in the macropores.Then, the sorption rate decreases because there are fewer sorption sites available, and the CPX concentration gradient decreases as the system evolves towards equilibrium.More than 3 days of contact time were required to reach the equilibrium.The contact time required to reach equilibrium varies widely depending on the nature of the adsorbate and adsorbent, the adsorbent particle size and the initial adsorbate concentrations.Reported equilibrium times for the adsorption of CPX include three days for adsorption on activated carbon, carbon xerogel and carbon nanotubes [1]; over five days for an initial concentration of 842 mg•L −1 and over two days for 84.2 mg•L −1 for adsorption on bamboobased activated carbon [7]; more than 24 h for adsorption on biochar from cassava dregs [15]; over nine hours with biochar from used tea leaves [16]; and 24 h for adsorption on pumpkin peel biochar prepared with phosphoric acid [31].A frequently used kinetic model to study the mechanism of diffusion during adsorption is intraparticle diffusion [6,16].As can be observed in Figure 4, this model does not adequately describe the adsorption kinetics of CPX on AB (ARE = 7.3) over the entire ex-  There are several steps involved in the adsorption of a solute onto the surface of an adsorbent solid: (1) adsorbate movement from the fluid phase to the boundary layer next to the adsorbent surface, (2) adsorbate diffusion through the boundary layer to the outer adsorbent surface, (3) adsorption on the active sites on the adsorbent surface, (4) the diffusion of the adsorbate into the pores of the adsorbent, and (5) adsorption on the active sites inside the pores.The overall rate at which adsorption occurs may be controlled by one or more of these steps.
To study the adsorption mechanism and establish the rate-controlling steps, the experimental data have been fitted to five kinetic models: pseudo-first order, pseudo-second order, Elovich, intraparticle diffusion and Bangham (Table 1).The fit to the kinetic models is shown in Figure 4 and the obtained characteristic parameters are listed in Table 3.
Table 3. Kinetic parameters for the sorption of CPX by AB.

Pseudo-First Order
Pseudo-Second Order Elovich Intraparticle Diffusion Bangham 38 A frequently used kinetic model to study the mechanism of diffusion during adsorption is intraparticle diffusion [6,16].As can be observed in Figure 4, this model does not adequately describe the adsorption kinetics of CPX on AB (ARE = 7.3%) over the entire experimental period.A plot of q t versus t 1/2 is shown in Figure 5.As can be seen, the overall adsorption process can be divided into two linear zones.This suggests that the rate of adsorption is controlled by several steps, and not only by intraparticle diffusion.The first linear zone has the steepest slope and is related to the diffusion of CPX through the boundary layer to the outer surface of the AB.Moreover, this first linear zone does not pass through the coordinate origin, suggesting that the adsorption rate at this initial stage is influenced by diffusion through the boundary layer.The second linear zone is related to intraparticle diffusion in the micropores and mesopores of the AB.The lower slope of this second zone indicates that there is a higher mass transfer resistance at this stage, and that CPX diffusion into the pores of AB is a slower process.

Effect of Initial Concentration and Adsorption Isotherm
The influence of the initial CPX concentrations, ranging from 35 mg•L −1 to 925 mg•L −1 , on the adsorption capacity and removal percentage was studied (Figure 6).An increase in the initial concentration leads to an increase in the adsorption capacity due to the increased concentration gradient between the solution and the adsorbent, while the removal percentage decreases because the number of available active sites remains constant and the competition for these sites increases.Thus, it can be indicated that the overall adsorption process is complex and involves both diffusion of CPX through the boundary layer and intraparticle diffusion in the AB pores.
Based on the lowest ARE values, it can be said that the Elovich and Bangham models adjusted the experimental data better than the other used models, and the sorption process can be described adequately by those two models (ARE values lower than 3.2%).The good fitting to the Elovich and Bangham models suggests the presence of the chemical adsorption of CPX on a heterogeneous sorbent surface, and that the limiting stage is the solute's diffusion within the pores [46,49].

Effect of Initial Concentration and Adsorption Isotherm
The influence of the initial CPX concentrations, ranging from 35 mg•L −1 to 925 mg•L −1 , on the adsorption capacity and removal percentage was studied (Figure 6).An increase in the initial concentration leads to an increase in the adsorption capacity due to the increased concentration gradient between the solution and the adsorbent, while the removal percentage decreases because the number of available active sites remains constant and the competition for these sites increases.

Effect of Initial Concentration and Adsorption Isotherm
The influence of the initial CPX concentrations, ranging from 35 mg•L −1 to 925 mg•L −1 , on the adsorption capacity and removal percentage was studied (Figure 6).An increase in the initial concentration leads to an increase in the adsorption capacity due to the increased concentration gradient between the solution and the adsorbent, while the removal percentage decreases because the number of available active sites remains constant and the competition for these sites increases.The experimental sorption isotherm (relationship between the concentration of CPX in a solution, ce, and the amount of CPX adsorbed per unit adsorbent, qe, at equilibrium) at 25 °C is shown in Figure 7.At low ce values (ce values between 0 and 150 mg•L −1 ), the sorption capacity increases significantly with increasing ce.Subsequently the qe values The experimental sorption isotherm (relationship between the concentration of CPX in a solution, C e , and the amount of CPX adsorbed per unit adsorbent, q e , at equilibrium) at 25 • C is shown in Figure 7.At low C e values (C e values between 0 and 150 mg•L −1 ), the sorption capacity increases significantly with increasing C e .Subsequently the q e values tend to reach a constant value, which is the maximum adsorption capacity (approximately 110 mg•g −1 ), although a plateau is not reached even at higher concentrations of CPX in the solution.
Five isotherm models have been used to fit the experimental equilibrium data: Langmuir, Freundlich, Sips, Redlich-Peterson and Toth.The fitted isotherm models are shown in Figure 7.The corresponding characteristic parameters and the ARE values were obtained (Table 4).
78.07 q mT (mg•g −1 ) 131.4  Five isotherm models have been used to fit the experimental equilibrium data: Langmuir, Freundlich, Sips, Redlich-Peterson and Toth.The fitted isotherm models are shown in Figure 7.The corresponding characteristic parameters and the ARE values were obtained (Table 4).As can be observed, the Freundlich model represents the equilibrium data very well and provides the best fit (lower ARE value, 2.4%).The Redlich-Peterson, Sips and Toth models are also suitable for describing the adsorption process, with ARE values of 3.3%, 4.9% and 5.4%, respectively.The Langmuir model is not suitable to describe CPX adsorption due to the high ARE value (11.9%).
The best fit to the Freundlich model suggests a multilayer adsorption on a heterogeneous sorbent surface.The low value of the Toth isotherm exponent (TT = 0.27) is in agreement with this statement.
The KF value (38.02 (mg•g −1 ) (mg•L −1 ) −1/n ) is related to the adsorption capacity.The n parameter is related to the intensity of adsorption, and if its value is greater than one, the adsorption is favourable [53].The obtained n value is 6.44, indicating that the sorption of CPX on this activated biochar is favourable.The best fit to the Freundlich model suggests a multilayer adsorption on a heterogeneous sorbent surface.The low value of the Toth isotherm exponent (T T = 0.27) is in agreement with this statement.
The K F value (38.02 (mg•g −1 ) (mg•L −1 ) −1/n ) is related to the adsorption capacity.The n parameter is related to the intensity of adsorption, and if its value is greater than one, the adsorption is favourable [53].The obtained n value is 6.44, indicating that the sorption of CPX on this activated biochar is favourable.
The constant (β) of the Redlich-Peterson model and the equilibrium parameter or Hall separation factor (R L ), calculated (Equation ( 3)) from the Langmuir model, are related to the feasibility of the adsorption process.
Adsorption is favourable for β and R L values between 0 and 1.In this study, the β value is 0.85 and the calculated R L values range from 0.03 to 0.44, indicating that CPX sorption on potassium carbonate activated biochar is favourable, as indicated by the n parameter of the Freundlich model.Furthermore, the adsorption is enhanced as the initial CPX concentration increases, as shown by the decrease in the R L value with increasing initial CPX concentrations.
The value of the Sips isotherm exponent, n S , is 3.24.Values of n S greater than 1 indicate that the behaviour of the Sips isotherm corresponds to the Freundlich model, and it qualitatively shows the heterogeneity of the adsorbent surface.
The values of the maximum adsorption capacity (q max ) obtained from the Sips and Toth models are 133.4mg•g −1 and 131.4 mg•g −1 , respectively.Although the Langmuir model gives the worst fit to the experimental data, it can roughly estimate the q max of the sorbent (104.9 mg•g −1 ).This value is similar to that expected from the experimental data (Figure 7).This value can be compared with other values of maximum adsorption capacity reported in the review by Malakootian et al. (2021) [9] for the adsorption of CPX on different sorbents.These values range from 47 ng•g −1 (birnessite) to 1575 mg•g −1 (C@silica core/shell nanoparticles of ZIF-8).When the sorbent is raw or activated biochar, the q max varies between 8.3 mg•g −1 (magnetic biochar based on a manganese oxide composite) and 449.4 mg•g −1 (magnetic biochar doped with ZnO from camphor leaves).For comparison, Table 5 shows typical values for the maximum sorption capacity of some biochars recently used for CPX removal.As can be seen, the K 2 CO 3 -activated biochar prepared from barley bagasse has an adsorption capacity intermediate between those reported for other biochars.
Table 5. CPX maximum adsorption capacities of various raw and modified biochars.

Figure 1 .
Figure 1.FTIR spectra of activated biochar before and after CPX adsorption.Figure 1. FTIR spectra of activated biochar before and after CPX adsorption.

Figure 1 .
Figure 1.FTIR spectra of activated biochar before and after CPX adsorption.Figure 1. FTIR spectra of activated biochar before and after CPX adsorption.

Figure 2 .
Figure 2. Determination of activated biochar's point of zero charge.

Figure 2 .
Figure 2. Determination of activated biochar's point of zero charge.

Figure 3 .
Figure 3.Effect of pH on CPX adsorption at equilibrium.The symbols are the average values, and the upper and lower bars indicate the standard deviation.Conditions: temperature = 25 °C; contact time = 72 h; sorbent dose = 0.5 g•L −1 ; C0 CPX = 300 mg•L −1 .

Figure 3 .
Figure 3.Effect of pH on CPX adsorption at equilibrium.The symbols are the average values, and the upper and lower bars indicate the standard deviation.Conditions: temperature = 25 • C; contact time = 72 h; sorbent dose = 0.5 g•L −1 ; C 0 CPX = 300 mg•L −1 .

Table 3 .
Kinetic parameters for the sorption of CPX by AB.

Table 4 .
Isotherm parameters for the sorption of CPX by AB.
As can be observed, the Freundlich model represents the equilibrium data very well and provides the best fit (lower ARE value, 2.4%).The Redlich-Peterson, Sips and Toth models are also suitable for describing the adsorption process, with ARE values of 3.3%, 4.9% and 5.4%, respectively.The Langmuir model is not suitable to describe CPX adsorption due to the high ARE value (11.9%).−1 ), although a plateau is not reached even at higher concentrations of CPX in the solution.

Table 4 .
Isotherm parameters for the sorption of CPX by AB.