Synthesis, Characterization, and Antibacterial Potential of Poly( o -anisidine)/BaSO 4 Nanocomposites with Enhanced Electrical Conductivity

: The poly(o-anisidine)/BaSO 4 nanocomposites were prepared by oxidative polymerization of o -anisidine monomer with BaSO 4 ﬁller for the potential antibacterial properties of the composite materials. To achieve the optimal and tunable properties of the nanocomposites, the ratio of BaSO 4 ﬁller was changed at the rates of 1%, 3%, 5%, 7%, and 10% with respect to matrix. Different analytical techniques, i.e., FTIR and UV-visible spectroscopy, were employed for functional identiﬁcation and optical absorption of the poly( o -anisidine)/BaSO 4 nanocomposites. The FTIR data revealed the signiﬁcant interaction between POA and BaSO 4 , as well as the good absorption behavior of the UV-visible spectra. The conducting properties were controllable by varying the load percentage of the BaSO 4 ﬁller. Furthermore, different bacterial strains, i.e., Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive), were used to evaluate the antibacterial activity of the POA/BaSO 4 nanocomposites. The largest zones of inhibition 0.8 and 0.9 mm were reached using 7% and 10% for Staphylococcus aureus and Pseudomonas aeruginosa , respectively.


Introduction
The development of nanomaterials has been a useful and important task during the last fifteen years. These nanomaterials have original multifunctionalities, which make them very helpful products in a variety of industries. The nanocomposites are classified into structural and functional materials. Structural nanocomposites focus on the improvement of mechanical properties due to the addition of nanoparticles, while for the functional ones, the presence of nanoparticles adds extra functionalities to the polymer, such as electrical conductivity, special optical properties, antimicrobial features, and many more. The hybrid nanomaterials consist of organic-inorganic components, e.g., organic polymer matrix and inorganic filler, and have potential applications as antimicrobial agents, biocompatible materials, sensors, and electrical devices. Moreover, they are utilized as high-value coatings in gas separation, ultra-and nanofiltration, and corrosion prevention, as well as in artificial membranes [1][2][3]. In addition, they can be used as catalysts, toxic-compound adsorbents, biomaterials, and information display materials with specialized optical, magnetic, and electrical capabilities [4][5][6].
These nanomaterials offer unique properties due to the fact that their components have a different nature to prepare some tailor-made novel nanomaterials [7][8][9][10]. In particular, the composites made by adding multi-walled carbon nanotubes to thin layers of poly (3,4-ethylenedioxythophene) and poly(4-styrenesulfonate) exhibited intriguing characteristics regarding the interdependence of electrical properties towards the large temperature range [11]. Thus, the inorganic and organic species interact at a molecular level at the nanoscopic range. Similarly, Mt-PS-BZO-PANI hybrid composites showed valuable luminescence properties [12]. Moreover, polyurethane (PU) and poly(hydroxyethyl methacrylate) composited with poly(titanium oxide) showed excellent viscoelastic and thermophysical properties [13]. The ability of these organic-inorganic hybrid materials to blend the heterogeneous properties of inorganic-organic components in a single material is its key benefit. In recent years, nanomaterials have offered one of the most interesting developments in the subject of materials chemistry [14][15][16].
The conducting polymer is one of a new class of polymers that are being used for a variety of applications. The merging of different unique properties, such as the chemical, electrical, and electrochemical properties of these polymers, may cause them to be used in several scientific applications [17][18][19][20]. Polyaniline has the unique property to coordinate with metallic ions, which provides the multi-metallic system and also prepares the nanocomposite materials with some other species. Polyaniline and its derivatives have achieved considerable attention over the last few years due to their conducting properties. The electro-active characteristics of polyaniline films, in particular, have been determined useful for the development of batteries, electrochromic displays, and microelectronics devices. Poly(o-anisidine) exhibits some interesting and unique properties [21][22][23][24]. Poly(o-anisidine) was found to have greater solubility compared with polyaniline in some organic solvents as it has a low boiling point while maintaining its crystalline property. This is very important because this polyaniline derivative can be used in technological applications [25][26][27][28][29].
Polymeric nanocomposites are modern and superior composites that are obtained from nanoparticles and the polymeric matrix, in which NPs are covered by polymers; through this, a core-shell arrangement can be developed [30]. Due to the particular shape, chemical nature, and exceptional structure of polymers, nanoparticles can be spread in a polymer matrix in the best and unique shapes. As a result of covering nanoparticles and their functionalization, the Van der Waals forces among NPs become reduced and the distribution of NPs in the matrix is improved and amplified [31,32]. The importance of polymer nanocomposites lies in the fact that polymers are always preferred for the covering of nanoparticles. On the other hand, appropriate functional groups in polymer structures can be used as reaction sites to organize the one-pot synthesis of nanocomposites [33,34].
Polymeric materials can be extensively employed in industries because of their lightweight and their simplicity of preparation, in addition to their elastic properties. Nevertheless, these materials have several drawbacks, such as their small modulus as well as potency compared to ceramics and metals [35]. Thus, to improve the mechanical properties, a very useful and valuable approach can be the addition of fibers, platelets, particles, or whiskers into such polymeric materials as reinforcements. The polymeric materials are composited with other filler materials to tune the properties of the materials, such as the temperature resistance, impact opposition, mechanical strength, fire or flame resistance, gas permeability, conductivity, and microbial resistance [36].
Due to the addition of reinforcements or filler materials to a wide range of polymer resins, their unique properties have greatly been improved. This is a great example of polymer-matrix nanocomposites acting as eco-friendly systems. Furthermore, the latest information on nano-industries' nanomaterials showed complete and effective applications in the form of polymer nanocomposites and has been successfully reported [31,37]. Moreover, the barium sulfate has versatile applications and can be utilized as an antibacterial agent, a paint, a film, a fiber, and a luminescence material, and for photocatalysis [38][39][40][41]. Consequently, the versatile properties of barium-sulfate-nanocomposite materials proved their widespread applications in every field. Thus, there exists a big and wonderful demand for such hybrid materials. Generally, these hybrid materials, based on organic polymers, have many advantages, such as good processability, long-term stability, and wonderful optical, electronic, and catalytic as well as magnetic properties [42,43]. Thus, the resulting nanocomposites could offer numerous applications in different fields, for example, antibacterial, optoelectronics, electrical devices, sensors, etc. [44]. Moreover, barium-sulfate nanoparticles show antibacterial properties due to its small size, greater surface area, and high penetration power to inhibit the growth of bacteria [43]. The barium-sulfate nanoparticles not only exhibit the antimicrobial properties towards the nanocomposite but also control the electrical conductivity of the materials. Therefore, the antibacterial properties of BaSO 4 nanoparticles as filler and conducting properties of the poly(o-anisidine) matrix were combined to obtain novel composite materials with bactericidal and conducting properties, and for biomedical and sensor applications.

Material and Methods
For the present research work, all the chemicals, such as ammonium persulfate [(NH 4 ) 2 S 2 O 8 ], o-anisidine monomer as an oxidant, diaminodiphenylamine (DDPA), HCl/H 2 SO 4 , DI water, barium chloride, and ammonium sulfate, were procured from the Sigma Aldrich company (St. Louis, MI, USA). They were chemicals of the analytical and research grade, and they were utilized directly.

Preparation of Poly(o-anisidine)
Using a chemical oxidative approach, the o-anisidine was polymerized into poly(oanisidine) ( Figure 1). Two solutions were prepared and named solution A and solution B using the conventional process. By combining 3 g of monomer with 10 mL of 1 M HCl, solution A was created. Then, with magnetic stirring, solution A was added to an ice bath. The next step was to create solution B, which was made by combining 4 g of ammonium persulfate with 10 mL of 1 M HCl and keeping it in an ice bath. Solution B was added dropwise in solution A, which was already constantly stirred. The reaction contents were continuously stirred for 2 h. After that, it was left undisturbed overnight. The bluishgreen suspension was prepared, which was subjected to centrifugation for isolation and purification. The material was isolated and washed with deionized water three times for better purification. Finally, the material was dried for 6 h at 80 • C in an electric oven and we proceeded with the characterization [17].

Preparation of Barium-Sulfate Nanoparticles
BaSO 4 nanoparticles were prepared by using the method already reported by Ahmad et al. [44]. The 20 mL of the BaCl 2 (0.5 M) solution, along with 20 mL absolute ethanol, was taken in a conical flask. Then, the (NH 4 ) 2 SO 4 solution (0.1 M) was prepared in distilled water. Then, the ammonium-sulfate solution was added dropwise to the conical flask containing the barium-chloride solution for 20 min with constant stirring at 25 • C. Afterwards, the gelatinous white barium-sulfate nanoparticles were formed, which were centrifuged to separate them from each other. Distilled water and ethanol were used to wash the item multiple times. Finally, the material was dried in an electric oven at 120 • C for 12 h. The dried material was collected to obtain the barium-sulfate nanoparticles. Processes 2022, 10, x FOR PEER REVIEW 4 of 13

Preparation of Barium-Sulfate Nanoparticles
BaSO4 nanoparticles were prepared by using the method already reported by Ahmad et al. [44]. The 20 mL of the BaCl2 (0.5 M) solution, along with 20 mL absolute ethanol, was taken in a conical flask. Then, the (NH4)2SO4 solution (0.1 M) was prepared in distilled water. Then, the ammonium-sulfate solution was added dropwise to the conical flask containing the barium-chloride solution for 20 min with constant stirring at 25 °C. Afterwards, the gelatinous white barium-sulfate nanoparticles were formed, which were centrifuged to separate them from each other. Distilled water and ethanol were used to wash the item multiple times. Finally, the material was dried in an electric oven at 120 °C for 12 h. The dried material was collected to obtain the barium-sulfate nanoparticles.

Synthesis of Poly(o-anisidine)/BaSO4 Nanocomposite
The preparation of the poly(o-anisidine)/BaSO4 nanocomposites was carried out by following the method of poly(o-anisidine) polymerization. Two solutions were prepared, which were designated as solution A and solution B. For solution A, 0.246 g of o-anisidine monomer was added in 20 mL of HCl (1 M), along with 0.026 g of the DDPA and BaSO4 nanoparticles. The load percentage of the BaSO4 nanoparticles was changed for different composites (1, 3, 5, 7, and 10), as shown in Table 1. Then, solution A was sonicated for 10 min and placed to stir in an ice bath. Additionally, 20 mL of HCl and 0.246 g of APS were combined to create solution B (1 M). After that, solution B was continuously stirred into solution A while being introduced dropwise in an ice bath. The mixture was stirred for 2 h and kept overnight for stabilization. The greenish suspension was obtained and we proceeded to the centrifugation. The material was separated and washed with distilled water three times. Then, the material was dried in an electric oven at 80 °C for 6 h [17]. Similarly, other composites were prepared by varying the percentage of the BaSO4 nanofiller according to the above method. Finally, the materials were collected and subjected to the characterization.  Table 1. Then, solution A was sonicated for 10 min and placed to stir in an ice bath. Additionally, 20 mL of HCl and 0.246 g of APS were combined to create solution B (1 M). After that, solution B was continuously stirred into solution A while being introduced dropwise in an ice bath. The mixture was stirred for 2 h and kept overnight for stabilization. The greenish suspension was obtained and we proceeded to the centrifugation. The material was separated and washed with distilled water three times. Then, the material was dried in an electric oven at 80 • C for 6 h [17]. Similarly, other composites were prepared by varying the percentage of the BaSO 4 nanofiller according to the above method. Finally, the materials were collected and subjected to the characterization.

Characterization
The samples were characterized by various analytical techniques, as mentioned below.

FTIR Spectroscopic Studies
For the FTIR spectroscopic studies, the Agilent carrying 630 spectrometers was used to conduct the FTIR analysis of the poly(o-anisidine)/BaSO 4 nanocomposites. The sample discs were prepared by using KBr powder and dried to remove any moisture at 80 • C in an electric oven. Then, the samples were analyzed by the FTIR spectrometer in the wavenumber range of 400-4000 cm −1 .

UV-Visible Spectroscopy
The UV-visible spectroscopic study of the poly(o-anisidine)/BaSO4 nanocomposites was carried out using the Shimadzu double-bean spectrophotometer. The samples were prepared by dispersing 1 mg of the composite in 5 mL of distilled water. Then, the samples were scanned in the wavelength range of 200-800 nm.

TEM Analysis
On the H-7650 (Hitachi, Tokyo, Japan), running at an acceleration voltage of 100 kV, the TEM examination was performed.

Conductivity Measurement
The CyberScan PC 510 conductivity meter was used to conduct the conductivity measurement of the poly(o-anisidine)/BaSO 4 nanocomposites. The samples were prepared by dispersing 1 mg of the composite in 5 mL of distilled water. Then, the dispersion was used for the measurement of electrical conductivity by the conductivity meter.

Antibacterial Activity
The antibacterial capability of the poly(o-anisidine)/BaSO4 nanocomposites was assessed using the disc-diffusion technique. Antibacterial testing was carried out by using the disc-diffusion method. Bacterial growth was carried out by using the nutrient-growth agar method. Only Gram-negative bacteria (Pseudomonas aeruginosa) were used in the MacConkey agar. Pseudomonas aeruginosa can grow on MacConkey but Staphylococcus aureus cannot grow on MacConkey. The media were autoclaved, chilled, and deposited in petri plates for 41 min. Forty-one minutes later, hardened agar plates were covered with a fresh inoculum (20 mL) of bacteria cultures. The UVAS diagnostic lab provided bacterial cultures. Every plate contained sterile paper discs dipped in various suspensions of the POA/BaSO 4 nanocomposites. For 24 h, the agar plates were incubated at 37 • C (310 K). The zones of inhibition were studied following a 24-hour incubation period. The magnitude of the presence or absence of growth inhibition zones was used to determine the sensitivity and resistance of bacteria in sensitivity tests.

Results and Discussion
The synthesized poly(o-anisidine)/BaSO 4 nanocomposites were characterized by various techniques, as mentioned above. Six samples were prepared, in which one was the pure polymer and other five were nanocomposites with BaSO 4 nanoparticles (1%, 3%, 5%, 7%, and 10%). The barium-sulfate nanoparticles were characterized by powder XRD and the average particle size was 25.26 nm, as reported by Ahmad et al. [44]. The composites were further analyzed by using the following analytical techniques.

FTIR Spectroscopic Studies
The FTIR analysis of the poly(o-anisidine)/BaSO 4 nanocomposites was carried out to determine the structural changes in the material. The C=C stretching mode of the quinoid ring appeared at 1591 cm −1 , which was attributed to the stretching vibration of the o-methoxy group. The poly(2-methoxy aniline) signal appeared at 3379 cm −1 , which was Processes 2022, 10, 1878 6 of 13 attributed to the N-H stretching mode. The C=C stretching modes of the benzenoid ring appeared at 1469, 1206, and 1032 cm −1 , which were attributed to the stretching vibration of the o-methoxy group. The methoxy band clearly indicated the existence of poly(o-methoxy aniline) in the spectra. The spectrum in Figure 2b shows the addition of 1% of the BaSO 4 nanoparticles in the poly(o-anisidine) matrix at 2837 cm −1 , demonstrating the interaction of the poly(o-anisidine) with the BaSO 4 that appeared as the peak was deeper and wider.
The FTIR analysis of the poly(o-anisidine)/BaSO4 nanocomposites was carried out determine the structural changes in the material. The C=C stretching mode of the quino ring appeared at 1591 cm −1 , which was attributed to the stretching vibration of the o-me oxy group. The poly(2-methoxy aniline) signal appeared at 3379 cm −1 , which was tributed to the N-H stretching mode. The C=C stretching modes of the benzenoid r appeared at 1469, 1206, and 1032 cm −1 , which were attributed to the stretching vibrat of the o-methoxy group. The methoxy band clearly indicated the existence of poly(o-me oxy aniline) in the spectra. The spectrum in Figure 2b shows the addition of 1% of BaSO4 nanoparticles in the poly(o-anisidine) matrix at 2837 cm −1 , demonstrating the int action of the poly(o-anisidine) with the BaSO4 that appeared as the peak was deeper a wider. The peak that appeared at 2837 cm −1 with the addition of 3 percent of the BaS nanoparticles in the poly(o-anisidine) matrix was practically the same as in the 1 perce indicating that the 3 percent addition of the BaSO4 nanoparticles improved the propert of the polymer. The peak at 2116 cm −1 indicated the interaction of the BaSO4 nanopartic with the polymer matrix when 3 percent of the BaSO4 nanoparticles was added to polymer matrix. The peak at 1568 cm −1 was linked to the quinoid ring's (C=C) stretch modes.
The characteristic peak was observed at 1266 cm −1 after the addition of 5% of BaSO4 nanoparticles in the polymer matrix, as shown in Figure 2d, indicating that th The peak that appeared at 2837 cm −1 with the addition of 3 percent of the BaSO 4 nanoparticles in the poly(o-anisidine) matrix was practically the same as in the 1 percent, indicating that the 3 percent addition of the BaSO 4 nanoparticles improved the properties of the polymer. The peak at 2116 cm −1 indicated the interaction of the BaSO 4 nanoparticles with the polymer matrix when 3 percent of the BaSO 4 nanoparticles was added to the polymer matrix. The peak at 1568 cm −1 was linked to the quinoid ring's (C=C) stretching modes.
The characteristic peak was observed at 1266 cm −1 after the addition of 5% of the BaSO 4 nanoparticles in the polymer matrix, as shown in Figure 2d, indicating that the 5 percent addition of the BaSO 4 nanoparticles increased the features of the polymer. The peak at 1628 cm −1 indicated the interaction of the BaSO 4 nanoparticles with the polymer matrix when 5 percent of the BaSO 4 nanoparticles was added to the polymer matrix. The peak at 1568 cm −1 was linked to the quinoid ring's (C=C) stretching modes.
The peak at 3511 cm −1 was, again, found by adding 7% of the BaSO 4 nanoparticles to the polymer matrix, as shown in Figure 2e, which was deep but not as wide as in the prior cases, indicating that the 7% addition of the BaSO 4 nanoparticles improved the polymer properties. Furthermore, the spectrum revealed that when 7% of the BaSO 4 nanoparticles are added to the polymer matrix, a peak at 1628 cm −1 indicates that the BaSO 4 nanoparticles are bound to the polymer matrix. The quinoid ring's (C=C) stretching modes were attributed to the peak at 1589 cm −1 .  Figure 2f shows the spectra of 10% of the BaSO 4 nanoparticles. It can be seen that due to the addition of 10% of the BaSO 4 nanoparticles in the poly(o-anisidine), a sharp and comparatively deep peak appeared at 3511 cm −1 , which showed the addition of 10% of BaSO 4 NPs, which increased the features of the poly(o-anisidine) differently. The peak that formed at 1626 cm −1 after the addition of 10% of the BaSO 4 nanoparticles to the poly(oanisidine) matrix illustrated the binding of BaSO 4 nanoparticles to the polymer matrix. The quinoid-ring stretching modes (C=C) were exhibited at 1589 cm −1 [30].

UV-Visible Spectroscopy
The POA/BaSO 4 nanocomposite was characterized to understand the absorption behavior of the POA/BaSO 4 nanocomposite. To analyze the absorption behavior of composite materials, the materials were scanned in the wavelength range of 200-800 nm at the rate of 400 nm/min under a UV-visible spectrophotometer. The spectrophotometer was furnished with UV Winlab programming to record and analyze the information. A blank reference was used to conduct baseline correction on the spectrophotometer. The absorption spectra of the pure POA and POA/BaSO 4 nanocomposites are shown in Figure 3. The significant absorption peak appeared at 273 nm, related to π-π* aromatic C=C band transitions and n-π* aromatic C=N band transitions. The o-anisidine monomer-to-monomer attachment was responsible for conjugation and polarity. The interaction of the BaSO 4 nanofiller and POA matrix resulted in a change in the absorption behavior of the composites. The absorption behavior of the composites was significantly different than that of the pure polymer, as shown in Figure 3a. There was a notable shifting of the absorption peak towards higher wavelengths, resulting in the marvel of the red shift at~520 nm. Moreover, the absorption intensity of the composites was much higher than that of the pure polymer, indicating the role of barium-sulfate filler in improving the absorption properties of the POA/BaSO 4 composites. The other composites also showed similar absorption behaviors, as depicted in Figure 3b-f. The absorption intensity was enhanced with the increase in the barium-sulfate-load percentage in the composites [17].

TEM Analysis
By using TEM micrograms at various magnifications to better examine the morphology of the BaSO 4 nanoparticles, Figure 4 was created. Due to their strong magnetic interaction and large surface area due to their nanoscale size, the produced BaSO 4 nanoparticles were found to be crystalline and include agglomerated irregular particles with an average size of 30-60 nm (Figure 4).

Conductivity Test
The POA/BaSO 4 nanocomposites were subjected to the measurement of electrical conductivity by the conductivity meter. The electrical conductivity of the nanocomposites depends upon the properties of the individual components and also depends on the temperature factor. Table 2 shows the electrical conductivity of POA at 1080 S/m. The electrical conductivities of the POA/BaSO 4 nanocomposites determined were lower than the pure POA. The nanocomposite with 1% of loading of the BaSO 4 nanoparticles showed conductivity at 992 S/m. Other nanocomposites containing 3%, 5%, 7%, and 10% of loading of BaSO 4 NPs exhibited conductivity at 919, 843, 784, and 578 S/m respectively ( Figure 5). These results not only confirm the presence of nanoparticles but also show that the increase in the percentage of the BaSO 4 nanoparticles in the composites results in a decrease in the conductivity of the materials [17]. The addition of barium sulfate in poly(o-anisidine) allowed the composite properties to be tunable, which can be used for designing the sensor materials for future use.

TEM Analysis
By using TEM micrograms at various magnifications to better examine the morphology of the BaSO4 nanoparticles, Figure 4 was created. Due to their strong magnetic interaction and large surface area due to their nanoscale size, the produced BaSO4 nanoparticles were found to be crystalline and include agglomerated irregular particles with an average size of 30-60 nm ( Figure 4).

TEM Analysis
By using TEM micrograms at various magnifications to better examine the ogy of the BaSO4 nanoparticles, Figure 4 was created. Due to their strong magn action and large surface area due to their nanoscale size, the produced BaSO4 n cles were found to be crystalline and include agglomerated irregular particle average size of 30-60 nm (Figure 4).   the percentage of the BaSO4 nanoparticles in the composites results in a d conductivity of the materials [17]. The addition of barium sulfate in poly(o lowed the composite properties to be tunable, which can be used for design materials for future use.

Disc-Diffusion Method
The synthesized poly(o-anisidine)/BaSO4 nanocomposites were evalu antibacterial activity against different strains of bacteria using the disc-d nique. It was found that poly(o-anisidine)/BaSO4 nanocomposites are effect ing the bacterial strains. The composites showed significant efficacy agains aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive). Th activity of the BaSO4 nanocomposites is noteworthy and reported in Table   0

Disc-Diffusion Method
The synthesized poly(o-anisidine)/BaSO 4 nanocomposites were evaluated for their antibacterial activity against different strains of bacteria using the disc-diffusion technique. It was found that poly(o-anisidine)/BaSO 4 nanocomposites are effective for inhibiting the bacterial strains. The composites showed significant efficacy against Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive). The antibacterial activity of the BaSO 4 nanocomposites is noteworthy and reported in Table 3. The POA/BaSO 4 nanocomposite that inhibited Pseudomonas aeruginosa growth was found to be optimal at 7% (Table 3 and Figure 6). Similarly, Staphylococcus aureus growth was inhibited by the POA/BaSO 4 nanocomposites. The maximum inhibition was associated with 10% of the POA/BaSO 4 nanocomposites against Gram-positive bacteria (Staphylococcus aureus). Nanoparticles have a small size, a larger surface area than bulky particles, and better penetration; therefore, they have a greater bactericidal effect. The specific process by which nanoparticles enter bacteria is unclear; however, studies have shown that treating bacterial cultures with nanoparticles alters the shape of the membranes and significantly increases membrane permeability, impairing normal transport through the plasma membrane. Bacterial cells die when they are unable to manage the transport across the plasma membrane. It was believed that due to their small size, barium-sulphate nanoparticles enter the bacterial cell membrane and link to functional groups of proteins, causing denaturation. They are also hypothesized to cause bacterial cell death by interacting with phosphorus and sulphur compounds, such as DNA. Total bacterial inhibition was proportional to BaSO 4 -nanoparticle concentrations [30]. It was found that the BaSO 4 nanoparticles have a significant antibacterial activity, which may be utilized for practical uses.
The POA/BaSO4 nanocomposite that inhibited Pseudomonas aeruginosa growth wa found to be optimal at 7% (Table 3 and Figure 6). Similarly, Staphylococcus aureus grow was inhibited by the POA/BaSO4 nanocomposites. The maximum inhibition was assoc ated with 10% of the POA/BaSO4 nanocomposites against Gram-positive bacteria (Stap ylococcus aureus). Nanoparticles have a small size, a larger surface area than bulky part cles, and better penetration; therefore, they have a greater bactericidal effect. The specif process by which nanoparticles enter bacteria is unclear; however, studies have show that treating bacterial cultures with nanoparticles alters the shape of the membranes an significantly increases membrane permeability, impairing normal transport through th plasma membrane. Bacterial cells die when they are unable to manage the transport acro the plasma membrane. It was believed that due to their small size, barium-sulphate nan particles enter the bacterial cell membrane and link to functional groups of proteins, cau ing denaturation. They are also hypothesized to cause bacterial cell death by interactin with phosphorus and sulphur compounds, such as DNA. Total bacterial inhibition wa proportional to BaSO4-nanoparticle concentrations [30]. It was found that the BaSO4 n noparticles have a significant antibacterial activity, which may be utilized for practic uses.

Conclusions
The current study provides significant results regarding the antibacterial and con ductivity properties of POA/BaSO4 nanocomposites. Poly(o-anisidine)/BaSO4 nanocomp sites were synthesized by the oxidative polymerization of o-anisidine and variable pe centages of BaSO4 nanoparticles. The nanocomposites were tested by using different cha acterization techniques, i.e., FTIR, UV, and conductivity and disc-diffusion methods. Th

Conclusions
The current study provides significant results regarding the antibacterial and conductivity properties of POA/BaSO 4 nanocomposites. Poly(o-anisidine)/BaSO 4 nanocomposites were synthesized by the oxidative polymerization of o-anisidine and variable percentages of BaSO 4 nanoparticles. The nanocomposites were tested by using different characterization techniques, i.e., FTIR, UV, and conductivity and disc-diffusion methods. The FTIR results show the strong communication among poly(o-anisidine) and BaSO 4 nanoparticles, and the UV-visible results indicate good absorption behavior. Moreover, the POA/BaSO 4 nanocomposites exhibited significant bactericidal potential against Pseudomonas aeruginosa and Staphylococcus aureus, and a tunable electrical conductivity feature. Therefore, the versatile properties of poly(o-anisidine)/BaSO 4 nanocomposites identify these materials as future candidates for biomedical, electrical device, and sensor applications.